CATALYSIS LETTERS
·
2022
·Vol. 152
·期 12
·3594-3606
·
IF 2.936
Yao Haoyang,Xu Yunlan,Zhong Dengjie,Zeng Yundong,Zhong Nianbing
In order to inhibit rapid recombination of electron–hole pairs of photoanode and expand its light response wavelength, PPy-14%/WO 3 /TiO 2 /Ti photoanode was prepared. The maximum photocurrent density is 2.31 mA·cm −2 , which is 1.76 times higher than that of WO 3 /TiO 2 /Ti at 1.1 V (vs. SCE). The maximum power density, phontocurrent density and Rhodamine B degradation rate of photocatalytic fuel cell (PFC) with PPy-14%/WO 3 /TiO 2 /Ti photoanode and Cu cathode are 25.48 μW·cm −2 , 0.25 mA·cm −2 and 88.5%, respectively. The excellent performance is mainly due to the good carrier migration rate of PPy and the synergistic effect of PPy/WO 3 /TiO 2 /Ti ternary hybrid electrode, so that photogenerated electrons and holes can accumulate in the conduction band of WO 3 and HOMO of PPy, respectively. This not only expands the light absorption range and enhances the light absorption intensity, but also improves the separation of electron–hole pairs, and finally significantly enhances the Rhodamine B degradation and electricity generation performance of PFC. Graphical Abstract In order to inhibit rapid recombination of electron–hole pairs of photoanode and expand its light response wavelength, PPy-14%/WO 3 /TiO 2 /Ti photoanode was prepared. The excellent performance is mainly due to the good carrier migration rate of PPy and the synergistic effect of PPy/WO 3 /TiO 2 /Ti ternary hybrid electrode, so that photogenerated electrons and holes can accumulate in the conduction band of WO 3 and HOMO of PPy, respectively.
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CHINESE JOURNAL OF POLYMER SCIENCE
·
2017
·Vol. 36
·期 1
·18-24
·
IF 1.692
Jing Xian-Wu,Huang Zhi-Yu,Lu Hong-Sheng,Wang Bao-Gang
A series of triblock copolymers, containing a CO 2 -switchable block poly(2-(dimethylamino)ethyl methacrylate) (PDM) block and two symmetrical hydrophilic blocks polyacrylamide (PAM), were synthesized using atom transfer radical polymerization (ATRP) method. The pH and conductivity tests showed that the triblock copolymer exhibited switchable responsiveness to CO 2 , i.e. a relatively low conductivity of solution could be switched on and off by bubbling and removing of CO 2 , and the triblock copolymer aqueous solution displayed a CO 2 -switchable viscosity variation. The changes were all attributed to protonation of tertiary amine groups in PDM blocks and proven by 1 H-NMR. Cryogenic transmission electron microscopy and dynamic light scattering characterization demonstrated that the viscosity variation was the result of a unilamellar vesicle-network aggregate structure transition. The release of rhodamine B from the vesicles with and without CO 2 stimuli showed the potential application in drug delivery domains; after CO 2 bubbling, the drug release rate could be accelerated. Finally, reasonable mechanism of CO 2 -switchable morphology changes and CO 2 -induced drug release was proposed.
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COLLOID AND POLYMER SCIENCE
·
2020
·Vol. 298
·期 8
·1053-1061
·
IF 1.536
Cai Shuwei,Gu Shiyu,Li Xian,Wan Songhan,Chen Songbo,He Xianru
A controlled grafting modification starch exhibited tunable phase transition temperature in aqueous solution was prepared via reversible addition–fragmentation chain transfer (RAFT) polymerization and esterification reaction under mild condition. P(AM/AN) copolymer was grafted onto the starch backbone and endowed the grafted copolymer thermosensitivity. The molecular structure and composition of P(AM/AN) copolymers were characterized by gel permeation chromatography (GPC), FTIR, and NMR, indicating a structurally controllable side chain of the grafted copolymer. Results of X-ray diffraction (XRD) demonstrate that the crystallinity of grafted copolymer decreases compared with starch due to side chains destruct the structural regularity of starch. Scanning electron microscopy (SEM) images show that grafted copolymer displays a rough, multilayer, and irregular morphology. Dynamic light scattering (DLS) and UV tests indicate that the grafted copolymer forms aggregates in aqueous solution and the UCST of the grafted copolymer is dependent on percent grafting (PG) and molecular interactions of side chains.
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Food Analytical Methods
·
2018
·Vol. 12
·期 2
·517-525
·
IF 2.245
Li Weixia,Chen Ning,Huang Zhongping,Zeng Xiuqiong,Zhu Yan
Antibiotic residue has become a serious issue of food safety, due to the emergence of drug-resistant bacteria and their toxic side effects. Therefore, regarding the need, we herein report a rapid, sensitive, and selective switchable hydrophilicity dispersive solvent-based liquid-liquid microextraction (SHDS-LLME) method combined with high-performance liquid chromatography and UV detector for determining amphenicols in food products. In the off-line extraction procedure, the mixture of extraction solvent (butanol) and dispersive solvent (tetraethylenepentamine) was manually injected into the acidic aqueous sample solution. Specifically, after the fast neutralization reaction, the dispersive solvent was converted into water-soluble salt and phase separation was achieved. Under the optimal conditions, a good linearity was observed in the range of 0.27–50.0 μg kg −1 . The limit of detection for chloramphenicol (CAP) and thiamphenicol (TAP) were 0.03 and 0.08 μg kg −1 , respectively. Recoveries for the spiked samples obtained were between 81.5 and 113.5% and the relative standard deviation was less than 8.6%. The proposed method was successfully applied for the analysis of amphenicols in four food products.
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Journal of Inorganic and Organometallic Polymers and Materials
·
2019
·Vol. 29
·期 5
·1625-1636
·
IF 1.637
Zhang Rui,Zhu Xuyang,Liu Yi,Cai Youfeng,Han Qiong,Zhang Tongqing,Li Yu
In this work, the versatile filler (HNTs) consisted of a halloysite nanotube as skeleton and intermediate magnetic nanoparticles coating poly(dopamine + 3-aminopropyltriethoxysilane) derivate layer were prepared. PVDF membranes were then manufactured by incorporating a series of ratios of HNTs. The Fourier-transform infrared (FT-IR), X-ray diffraction (XRD), thermogravimetric analysis (TGA) and transmission electron microscope (TEM) results proved that the modified materials were successfully achieved. The morphologies of membranes were characterized by scanning electron microscope (SEM) and atom force microscope (AFM). Meanwhile the PVDF hybrid membranes exhibited a stronger hydrophilicity (contact angle is 53°), higher bovine serum albumin (BSA) rejection (99%) than pure membrane (87°and 54%), and hybrid membranes maintain a higher flux recovery rate (FRR) that attains 85.3% after two cycles. Besides, the composite membrane showed a good removal rate (97.2%) of methylene blue. According to above experimental parameters, the 6% (based on PVDF mass) HNTs composite membrane was defined as the experimental optimal selection, and it could provide a promising candidate for methylene blue solution separation.
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MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 9
·3487-3496
·
IF 4.58
Sun Duanping,Lu Jing,Wang Xiangyan,Zhang Yuanqing,Chen Zuanguang
The authors describe a method for the detection and determination of human liver cancer cells in blood. The cytosensing system consists of a microfabricated chip-based electrochemical aptasensor that contains multifunctional hybrid electrochemical nanoprobes and an indium tin oxide (ITO) electrode array interface functionalized with cell-targeting aptamer and gold nanoparticles (AuNPs). The thiolated cell targeting aptamer (referred to as TLS11a) was immobilized on the ITO electrode/AuNPs for specific adhesion of hepatocellular carcinoma cells (HepG2). The hybrid nanoprobe system consists of hydroquinone (HQ) as an electrochemical probe, horseradish peroxidase (HRP), and an aptamer/hemin/G-quadruplex aggregate that was immobilized on gold/palladium-functionalized ZnO nanorods (ZnO@Au-Pd). The nanoprobes are capable of amplifying the voltammetric signal and capturing the target cells. Best operated at around −90 mV (vs Ag/AgCl), the electrode has a linear response that covers the 10^2 to 10^7 HepG2 cells per mL concentration range, with a 10 cell per mL detection limit. Captured cells may be released from the electrode via electrochemical desorption to break the Au-S bonds.
Schematic of an indium tin oxide (ITO) electrode array-based electrochemical aptamer cytosensor for the specific capture, sensitive detection and controlled release of HepG2 cancer cells. AuNP: gold nanoparticle; HQ: hydroquinone; HRP: horseradish peroxidase.
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MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 5
·1-9
·
IF 5.833
Wu Mingmin,Zhang Mengtian,Fan Zhiwei,Qin Xinyue,Zhu Xiaoxia,Ji Haiwei,Qin Yuling,Wang Qi,Wu Li
Sensitive and accurate detection of DNA methyltransferase (MTase) is conducive to the understanding of the fundamental biological processes related to DNA methylation, clinical disease diagnosis, and drug discovery. Herein, a new fluorescence transducer based on Förster resonance energy transfer (FRET) between the donor upconversion nanoparticles (UCNPs) and the efficient acceptor gold nanorods (AuNRs) for MTase activity analysis and its inhibitor screening is presented. A double-strand DNA linker between UCNPs and AuNRs could be digested by restriction endonuclease Hha I, preventing the FRET process and recovering the upconversion luminescence (UCL) intensity. With the treatment of MTase, the cutting site was disturbed by the methylation of cytosine, blocking the enzyme digestion. The transducer presented here showed an excellent analytical performance toward MTase M. Hha I in the concentration range 0.08~24 U mL −1 with a detection limit of 0.057 U mL −1 calculated according to the UCL intensity changes at 656 nm excited by 980 nm CW laser, which is superior to most of the reported methods. Furthermore, the as-fabricated transducer also demonstrated high testing and screening capability toward enzyme inhibitors’ evaluation. The method takes the advantage of low background fluorescence of UCNPs to improve the accuracy of the measurement, which can be developed as a general strategy for the analysis of various disease-related methyltransferase activity and their corresponding inhibitors, offering a promising strategy for high-performance diagnosis, high-efficient drug exploitation, and treatment effectiveness evaluation. Graphical abstract
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PubMed
高影响因子
Biotechnology for Biofuels
·
2019
·Vol. 12
·期 1
·1-18
·
IF 5.452
Yi Xia,Gao Qiuqiang,Zhang Lei,Wang Xia,He Yanqing,Hu Fengxian,Zhang Jian,Zou Gen,Yang Shihui,Zhou Zhihua,Bao Jie
Background Fast, complete, and ultimate removal of inhibitory compounds derived from lignocellulose pretreatment is the prerequisite for efficient production of cellulosic ethanol and biochemicals. Biodetoxification is the most promising method for inhibitor removal by its unique advantages. The biodetoxification mechanisms of a unique diploid fungus responsible for highly efficient biodetoxification in solid-state culture was extensively investigated in the aspects of cellular structure, genome sequencing, transcriptome analysis, and practical biodetoxification.Results The inborn heterozygous diploid structure of A. resinae ZN1 uniquely contributed to the enhancement of inhibitor tolerance and conversion. The co-expression of gene pairs contributed to the enhancement of the degradation of lignocellulose-derived model inhibitors. The ultimate inhibitors degradation pathways and sugar conservation were elucidated by microbial degradation experimentation as well as the genomic and transcriptomic sequencing analysis.Conclusion sThe finding of the heterozygous diploid structure in A. resinae ZN1 on biodetoxification took the first insight into the global overview of biodetoxification mechanism of lignocellulose-derived inhibitors. This study provided a unique and practical biodetoxification biocatalyst of inhibitor compounds for lignocellulose biorefinery processing, as well as the synthetic biology tools on biodetoxification of biorefinery fermenting strains.
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PubMed
高影响因子
NEUROCHEMICAL RESEARCH
·
2018
·Vol. 43
·期 12
·2435-2445
·
IF 2.772
Zhang Man,Huang Li-Li,Teng Chen-Huai,Wu Fang-Fang,Ge Li-yun,Shi Yu-Juan,He Zheng-Le,Liu Lei,Jiang Cheng-Jie,Hou Ruo-Nan,Xiao Jian,Zhang Hong-Yu,Chen Da-Qing
Traumatic brain injury (TBI) is a serious public health and medical problem worldwide. Oxidative stress plays a vital role in the pathogenesis of TBI. Nuclear factor erythroid 2-related factor 2 (Nrf2), an important factor in the cellular defense against oxidative stress, is activated following TBI. In this study, the protective effects of Isoliquiritigenin (ILG), a promising antioxidant stress drug, was evaluated as a protective agent against TBI. In a mouse model of controlled cortical impact Injury, we found that the ILG administration reduced the Garcia neuroscore, injury histopathology, brain water content, cerebral vascular permeability, the expression of cleaved caspase3, aquaporin-4, glial fibrillary acidic protein and the increased the expression of neurofilament light chain protein, indicating the protective effects against TBI in vivo. ILG treatment after TBI also restored the oxidative stress and promoted the Nrf2 protein transfer from the cytoplasm to the nucleus. We then used Nrf2−/− mice to test the protective effect of Nrf2 during ILG treatment of TBI. Our findings indicated that Nrf2−/− mice had greater brain injury and oxidative stress than wild-type (WT) mice and ILG was less effective at inhibiting oxidative stress and repairing the brain injury than in the WT mice. In vitro studies in SY5Y cells under oxygen glucose deprivation/re-oxygenation stimulation yielded results that were consistent with those obtained in vivo showing that ILG promotes Nrf2 protein transfer from the cytoplasm to the nucleus. Taken together, our findings demonstrate that Nrf2 is an important protective factor against TBI-induced injuries, which indicates that the protective effects of ILG are mediated by inhibiting oxidative stress after TBI via a mechanism that involves the promotion of Nrf2 protein transfer from the cytoplasm to the nucleus.
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Nanoscale Research Letters
·
2019
·Vol. 14
·期 1
·1-10
·
IF 3.159
Zheng Qingkai,Cheng Wenjing,Zhang Xiaoping,Shao Runxia,Li Zhongdong
In this report, we present a pH-induced reversible assembly system (PIRAS) based on ferritin (Ft) for targeted tumor therapy. It has been developed to easily load and release of the anticancer drug resveratrol (RV), based on its natural pH-sensitivity and unique hollow cavity of Ft. A tumor-specific target peptide Arg-Gly-Asp (RGD) was conjugated onto the surface of RV-loaded Ft (RV@Ft), to form biocompatible nanoparticles (RV@Ft-RGD). The pH-sensitivity of Ft allows it to be denatured into a hollow porous nanosphere under acidic condition and renatured into a sealed hollow nanosphere under neutral condition. Using pH manipulation, RV@Ft-RGD, with a ~ 21 nm diameter, showed a high RV loading ratio of 79.6%. pH-triggered RV release was then measured at a ratio of 50.3% at pH5.0 over 24 h. Under neutral condition, the RV@Ft-RGD showed excellent stability over 20 days. Confocal fluorescence imaging showed that RV@Ft-RGD had a high cell uptake ratio and co-localization with the lysosome, mainly due to the RGD-mediated target effect. Based on the high drug loading, pH-triggered release, and tumor cell targeting effect, RV@Ft-RGD showed great cell-killing ability in vitro and in vivo. The biocompatibility in vitro and in vivo was also demonstrated to be excellent, without systematic toxicity. The design concept of PIRAS based on Ft significantly inhibits tumor growth and simultaneously further broadens the application of Ft in nanomedicine.
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高影响因子
Scientific Reports
·
2023
·Vol. 13
·期 1
·1-11
·
IF 4.6
Li Hongwei,Chen Xin,Ren Kang,Wu Lihao,Chen Gong,Xu Ling
Diabetes may leave patients more prone to skin problems, and minor skin conditions can more easily turn into serious damage to the extracellular matrix, which further impairs the skin's mechanical properties and delays wound healing. Therefore, the aim of the work is to develop extracellular matrix substitution to remodel the mechanical properties of diabetic cutaneous wound and thus accelerate diabetic wound healing. A green fabrication approach was used to prepare radiation crosslinked bilayer collagen scaffold from collagen dispersion. The morphological, mechanical and swelling characteristics of radiation crosslinked bilayer collagen scaffold were assessed to be suitable for cutaneous wound remodeling . The feasibility of radiation crosslinked bilayer collagen scaffold was performed on full-skin defect of streptozotocin-induced diabetic rats. The tissue specimens were harvested after 7, 14, and 21 days. Histopathological analysis showed that radiation crosslinked bilayer collagen scaffold has beneficial effects on inducing skin regeneration and remodeling in diabetic rats. In addition, immunohistochemical staining further revealed that the radiation crosslinked bilayer collagen scaffold could not only significantly accelerate the diabetic wound healing, but also promote angiogenesis factor (CD31) production. Vascularization was observed as early as day 7. The work expands the therapeutic ideas for cutaneous wound healing in diabetes.
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高影响因子
JOURNAL OF NANOPARTICLE RESEARCH
·
2018
·Vol. 20
·期 11
·1-10
·
IF 2.127
Gu Fenfen,Hu Chuling,Xia Qingming,Gong Chunai,Gao Shen,Chen Zhongjian
Early diagnosis is primarily important for the therapeutic and prognostic outcomes of malignancies including prostate cancer (PCa). However, the visuality and veracity of ultrasound imaging for the diagnosis and prognostic prediction of PCa remains poor at present. In this study, we developed a new nanoultrasound contrast agent by modifying multi-walled carbon nanotubes (MWCNTs) with polyethylene glycol (PEG) and anti-PSMA aptamer. The result showed that the modified MWCNTs offered better visuality and veracity and were able to target PCa cells more effectively as compared with the traditional contrast agent. The zeta potential was about − 38 mv. The length of this contrast agent was about 400 nm and the diameter of it was about 30 nm. The zeta potential, TEM, and FT-IR all proved the successful preparation of the agent. The vitro cytological study revealed good cell uptake and biocompatibility of the new contrast agent. The minimum detection concentration in vitro is 10 μg/ml. The earliest stage of the detection was under the parameters of frequency = 6.0 MHz and medical index = 0.06. Both in vitro and in vivo ultrasound imaging demonstrated that the new nanoultrasound contrast agent had a good development effect, distribution, and metabolism, and may prove to be a good targeted ultrasound contrast agent, especially for PCa.
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