Polymer Chemistry
·
2012
·Vol. 4
·期 1
·156-165
·
IF 5.321
Lejiao Jia,Zhenyu Li,Dianrui Zhang,Qiang Zhang,Jingyi Shen,Hejian Guo,Xiaona Tian,Guangpu Liu,Dandan Zheng,Lisi Qi
The chitosan oligosaccharide-based disulfide-containing polyethylenimine derivative PEG-ss-COS-ss-PEI was synthesized and evaluated as a nonviral gene delivery carrier. The structure of the obtained polymers was confirmed by 1H NMR and FTIR. PEG-ss-COS-ss-PEI copolymers could effectively condense DNA into small particles with average diameters less than 120 nm and the zeta potential of +15.7 mV at the N/P ratio of 15/1. Additionally, the resultant polyplexes showed excellent colloidal stability against 150 mM NaCl and had a better buffering capacity of ∼44%, which was more than double the buffering capacity of PEI1.8k (∼20%). In the presence of 10 mM glutathione (GSH), however, polyplexes of PEG-ss-COS-ss-PEI were rapidly unpacked, as revealed by significant increase of particle sizes to over 800 nm. In vitro experiments revealed that the PEG-ss-COS-ss-PEI copolymers not only had much lower cytotoxicity, but also displayed high transfection efficiency as compared to the control branch 25 kDa PEI. This study indicates that a reducibly degradable copolymer PEG-ss-COS-ss-PEI composed of low molecular weight PEI, chitosan oligosaccharide and PEG via disulfide-containing linkages can be a promising gene delivery carrier.
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Biomedical Materials
·
2010
·Vol. 5
·期 6
·065016
·
IF 1.963
Deming Xie,Huamei Huang,Keith Blackwood,Sheila MacNeil
Both chitosan and polylactide/polyglycolide have good biocompatibility and can be used to produce tissue engineering scaffolds for cultured cells. However the synthetic scaffolds lack groups that would facilitate their modification, whereas chitosan has extensive active amide and hydroxyl groups which would allow it to be subsequently modified for the attachment of peptides, proteins and drugs. Also chitosan is very hydrophilic, whereas PLGA is relatively hydrophobic. Accordingly there are many situations where it would be ideal to have a copolymer of both, especially one that could be electrospun to provide a versatile range of scaffolds for tissue engineering. Our aim was to develop a novel route of chitosan-g-PLGA preparation and evaluate the copolymers in terms of their chemical characterization, their performance on electrospinning and their ability to support the culture of fibroblasts as an initial biological evaluation of these scaffolds. Chitosan was first modified with trimethylsilyl chloride, and catalyzed by dimethylamino pyridine. PLGA-grafted chitosan copolymers were prepared by reaction with end-carboxyl PLGA (PLGA–COOH). FT-IR and1H-NMR characterized the copolymer molecular structure as being substantially different to that of the chitosan or PLGA on their own. Elemental analysis showed an average 18 pyranose unit intervals when PLGA–COOH was grafted into the chitosan molecular chain. Differential scanning calorimetry results showed that the copolymers had different thermal properties from PLGA and chitosan respectively. Contact angle measurements demonstrated that copolymers became more hydrophilic than PLGA. The chitosan-g-PLGA copolymers were electrospun to produce either nano- or microfibers as desired. A 3D fibrous scaffold of the copolymers gave good fibroblast adhesion and proliferation which did not differ significantly from the performance of the cells on the chitosan or PLGA electrospun scaffolds. In summary this work presents a methodology for making a hybrid material of natural and synthetic polymers which can be electrospun and reacts well as a substrate for cell culture.
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Nanoscale Research Letters
·
2019
·Vol. 14
·期 1
·1-11
·
IF 3.159
Cheng Ziting,Zhang Wei,Hou Xiaoya,Wang Bingjie,Zhu Yanping,Zhang Peng,Zhao Feng,Chen Daquan
In this paper, a type of phycocyanin (PC)-functionalized and curcumin (CUR)-loaded biotin-chitosan oligosaccharide-dithiodipropionic acid-curcumin (BCSC) nanoparticles, called CUR-BCSC@PCs, were designed to enhance the biocompatibility of CUR. The structure of BCSC was confirmed using 1 H-NMR. In CUR-BCSC@PCs with an average hydrodynamic diameter of 160.3 ± 9.0 nm, the biomimetic protein corona gave the nanoparticles excellent stability and the potential to avoid protein adsorption in blood circulation. The in vitro release experiment verified that CUR-BCSC@PCs with redox responsive shells were sensitive to high concentrations of glutathione. In addition, CUR-BCSC@PCs were effective at increasing the inhibitory activity on the proliferation of A549 cells by enhancing the intracellular uptake of CUR. These results indicated that CUR-BCSC@PCs have great application prospects in cancer therapy as effective drug delivery carriers.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2019
·
IF 3.131
Xiaomin Tang,Ting Huang,Shixin Zhang,Wei Wang,Huaili Zheng
Hematite wastewater containing dissolved heavy metals is a potential threat to eco-environment and human health. Flocculation as an efficient and cost effective water treatment technology should be applied in its treatment. A sulfonated chitosan-based flocculant (CS-g-P(AM-AMPS)) was prepared via graft copolymerization. Two synthesis pathways and reaction products with different molecular structure were discovered in processes. CS-g-P(AM-AMPS) possessed the multifarious functional groups that contributed to its excellent performance in hematite wastewater treatment. Bridging, adsorption and entrapment in coagulation-flocculation were enhanced due to CS-g-P(AM-AMPS), and the impressive floc size and high removal efficiency were represented in the treatment as well. Dissolved heavy metals in the wastewater were removed by the chelation of sulfonate group in CS-g-P(AM-AMPS), the adsorption of other functional groups and the co-settlement with flocs. CS-g-P(AM-AMPS) will have the potential application in industrial wastewater treatment for its high flocculation performance, thermal stability and solubility.
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Journal of Materials Chemistry B
·
2016
·Vol. 4
·期 13
·2253-2263
·
IF 4.872
Mingxian Liu,Yanzhou Chang,Jing Yang,Yuanyuan You,Rui He,Tianfeng Chen,Changren Zhou
Halloysite nanotubes (HNTs) have a unique tubular structure in nanoscale, and have shown potential as novel carriers for various drugs. Coating the nanotubes with a hydrophilic polymer shell can significantly decrease the toxicity and provide colloidal stability during blood circulation. Here, we synthesized chitosan grafted HNTs (HNTs-g-CS) and investigated their potential as a nano-formulation for the anticancer drug curcumin. The structure and properties of HNTs-g-CS were characterized using water contact angle, zeta-potential, Fourier transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA), and transmission electron microscopy (TEM) techniques. HNTs-g-CS exhibit a maximum 90.8% entrapment efficiency and 3.4% loading capacity of curcumin, which are higher than those of raw HNTs. HNTs-g-CS also show no obvious hemolytic phenomenon and good stability in serum. The cumulative release ratio of curcumin from HNTs-g-CS/curcumin at cell lysate after 48 hours is 84.2%. The curcumin loaded HNTs-g-CS show specific toxicity to various cancer cell lines, including HepG2, MCF-7, SV-HUC-1, EJ, Caski and HeLa, and demonstrate an inhibition concentration of IC50 at 5.3–192 μM as assessed by cytotoxicity studies. The anticancer activity of this nanoformulation is extremely high in EJ cells compared with the other cancer cell lines. The cell uptake of HNTs-g-CS is confirmed by fluorescence microscopy. Flow cytometric analysis of curcumin loaded HNTs-g-CS shows that curcumin loaded HNTs-g-CS increase apoptosis on EJ cells. The content of ROS created by HNTs-g-CS/curcumin is more than that of free curcumin. All these results suggest that HNTs-g-CS are potential nanovehicles for anticancer drug delivery in cancer therapy.
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NORDIC PULP & PAPER RESEARCH JOURNAL
·
2023
·Vol. 38
·期 3
·413-423
·
IF 1.2
Jie Li,Haitang Liu,Qian Wang,Yefan Wang,Lin Chen,Xin Jin,Zhihua Guo,Ting Wang,Xinyu Zhang
The hemicellulose was extracted from the Stachys floridana Shuttlew. ex Benth by ultrasonic-assisted, fractional alcohol precipitation method, mixed with chitosan solution, and coated on the surface of the paper with three layers, to explore the effect of its morphology, thermal stability, barrier, mechanical properties of coated paper. The results showed that the gas barrier property of the paper coated with hemicellulose – chitosan mixed solution decreased from 180 mL/min to 0.265 mL/min, the water barrier property increased from 0 to 84.8°, and the oil barrier property increased from 0 to Kit nº11. The tearing degree of paper increased by 9.2 %, the folding resistance increased by 1296.6 %, dry and wet tensile strength and dry and wet rupture resistance were also significantly improved. The thermal stability mixed solution coated paper is slightly lower than the base paper, but higher than the pure chitosan coated paper. The prepared S. floridana Shuttlew. ex Benth hemicellulose – chitosan blend coating has good barrier property and mechanical properties, and has a wide application prospect.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Yilin Wang,Xuelian Huang,Chao He,Yupei Li,Weifeng Zhao,Changsheng Zhao
Safe and efficient carboxymethyl chitosan (CMC)-based heparin-mimicking cross-linked beads (CMC/PAMPS) as adsorbents for the clearance of low-density lipoprotein-cholesterol (LDL-c) in blood purification were prepared through hydrogen bonding interactions followed with in situ cross-linking with 2-acrylamido-2-methyl-1-propanesulfonicacid (AMPS). Fourier transform infrared spectra (FTIR), two-dimensional correlation FTIR spectroscopy (2D IR), thermal gravimetric analysis (TGA) and energy dispersive X-ray spectrometer (EDS) demonstrated the successful synthesis of CMC/PAMPS beads. The porous structures of the beads may contribute to the adsorption of toxins. Owing to their favorable hydrophilicity, when contacted with blood, the beads showed excellent hemocompatibility. The hemolysis ratios for all the beads were less than 5%, the thromboplastin time of CMC/PAMPS 10 beads exceed 600 s, and they can suppress contact activation and complement activation effectively. Additionally, the beads have no obvious cytotoxicity with endothelial cells, thus could be used as a safe adsorbent for blood purification. Moreover, the CMC/PAMPS 10 beads possessed a LDL-c adsorption capacity of 24.8 mg/g under static adsorption and a LDL-c adsorption capacity of 8.2 mg/g in the simulation of hemoperfusion for whole blood, due to the large surface areas and high density of functional groups. Meanwhile, the concentration of high-density lipoprotein-cholesterol (HDL-c) was almost unaffected. In general, the safe adsorbent with high LDL-c adsorption capacity has great potentials for hemoperfusion and other clinical applications.
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Materials Science & Engineering C-Materials for Biological Applications
·
2020
·
IF 5.88
Xiaoni Li,Pu Xu,Yanan Cheng,Wenbai Zhang,Bin Zheng,Qiuzi Wang
Bone tissue engineering using osteoinductive scaffolds has evolved into a promising approach for bone regeneration. Pearl powder has recently gained interest in bone regeneration due to their bioactive characteristics and favorable mechanical properties. In order to mimic bone tissue structurally and compositionally, chitosan-hyaluronic acid (C-HA) scaffolds containing nano-pearl powder (NPP) were fabricated in current study by using freeze-drying method. The microstructure, porosity, hydrophilia, and mechanical property of scaffolds were studied by scanning electron microscopy (SEM), mercury porosimeter, contact angles, and universal material experiment machine respectively. The crystal form of NPP, which was packed in scaffolds, was tested by X-ray diffraction (XRD), and the interaction between three constituents was studied by Fourier transformed infrared (FTIR). In the other hand, the biocompatibility, osteogenic characteristics, and osteogenic related gene expression of scaffolds were examined by cell counting kit-8 (CCK-8), alkaline phosphatase activity (ALP), RT-qPCR, and Western blotting. The results revealed that the NPP was successfully incorporated in the C-HA scaffolds. The hydrophilia and mechanical properties of scaffolds increased with NPP content, whereas the pore morphology was disturbed, especially for 25% scaffold. The scaffolds had a high porosity between 89% and 93%, and decreased slightly with the increase of NPP. Cell culture tests indicated that scaffolds with higher NPP proportion were beneficial for the proliferation and differentiation of MC3T3-E1 cells, and scaffolds with 10 wt% and 25 wt% nano-pearl powder were most effective. The differentiation might be promoted by upregulating the expression of Col αI, OCN, OPN and Runx2 genes. Therefore, the nano-pearl powder/chitosan-hyaluronic acid (NPP/C-HA) scaffolds may serve as a promising biomaterial for bone tissue engineering.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Xiaomin Zhang,Yajin Pan,Shengke Li,Lian Xing,Shoukang Du,Guoliang Yuan,Jianliang Li,Tianle Zhou,Dangsheng Xiong,Huaping Tan,Zhonghua Ling,Yong Chen,Xiaohong Hu,Xiaohong Niu
Biopolymer-based hydrogels with sustained drug release capability and antibacterial activity have exhibited great potential in clinical application in drug delivery and wound healing. In this study, a new type of composite wound dressing hydrogel aiming at avoiding wound infection was developed through embedding drug loaded gellan gum microspheres (GMs) into a doubly crosslinked hydrogel, which was constructed by Schiff-base crosslinking of oxidized gellan gum (OG) (pre-crosslinked by calcium ion) and carboxymethyl chitosan (CMCS). The gelation time, swelling index, degradation rate and mechanical properties of the blank hydrogel was optimized by varying the ratios of CMCS/OG ( w /w) with fixed OG/calcium (w/w) ratio. The best overall performance of the hydrogel was obtained when CMCS/OG is 16/7 (w/w), with a 139 s gelation time, swelling index remained above 30 after swelling equilibrium, 100.5% degradation rate on the seventh day, and 8.8 KPa compressive modulus. After being embedded with cargo-loaded GMs, the aforementioned performance of the blank hydrogel was improved, and the sustained release of cargoes (antibacterial drugs, tetracycline hydrochloride and silver sulfadiazine) was observed. Moreover, the excellent antibacterial activity of the composite hydrogel was also demonstrated in vitro . These results support the bioactive composite hydrogel can be employed as a promising injectable scaffold for promoting wound regeneration and drug delivery.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2014
·
IF 3.096
Liu Yang,Shiya Gao,Sajid Asghar,Guihua Liu,Jue Song,Xuan Wang,Qineng Ping,Can Zhang,Yanyu Xiao
The aim of this work was to evaluate the potential of polyelectrolyte complex nanoparticles (PENPs) based on hyaluronic acid/chitosan (HA/CS) as carriers for water-insoluble curcuminoid (CUR) and explore in vitro performance against brain glioma cells. PENPs were observed to be affected by the order of addition, mass ratios and initial concentrations of the HA/CS, pH and ionic strength . PENPs remained stable over a temperature range of 5–-55 (C. CUR was successfully encapsulated into the PENPs. CUR-PENPs showed spherical shape with a mean diameter of 207 nm and positive charge of 25.37 mV. High encapsulation efficiency (89.9%) and drug loading (6.5%) was achieved. Drug release studies revealed initial burst release of drug from the PENPs up to 4 h followed by sustained release pattern. DSC thermograms and XRD patterns showed that CUR was encapsulated inside the PENPs in a molecular or amorphous state. Compared with CUR-solution, CUR-PENPs showed stronger dose dependent cytotoxicity against C6 glioma cells and higher performance in uptake efficiency in C6 cells. Cellular uptake of CUR-PENPs was found to be governed by multi-mechanism in C6 cells, involving active endocytosis , macropinocytosis , clathrin-, caveolae-, and CD44-mediated endocytosis . In conclusion, CUR-PENPs might be a promising carrier for therapy of brain gliomas.
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CARBOHYDRATE POLYMERS
·
2018
·
IF 5.158
Wei Xiong,Guan Ding Zhao,Xunqing Yin,Ke Gang Linghu,John M.T. Chu,Gordon T.C. Wong,Haifeng Li,Hua Yu,Yi Tao Wang
Chitosan (CS), a nature-derived polysaccharide, is a promising nano-carrier material with good biocompatibility and biodegradability. However, the biomedical applications of CS are hindered because of its poor aqueous solubility. To circumvent this drawback, a series of Brij-grafted-chitosan copolymers (BCs) with various grafting degree of Brij-S20 were first developed and reported. The results indicated that the water solubility of BCs (from 9.13 to 9.54 mg/mL) were much higher than that of CS (0.32 mg/mL), due to the broken intra- and/or inter-molecular hydrogen bonds and the decreased initial crystallinity in BCs. The amphiphilic structure of BCs presented lower critical micelle concentration (0.116–0.376 mg/mL) thus facilitating its self-aggregation into micelles for drug encapsulation. Moreover, BCs markedly enhanced the intracellular uptake of rhodamine-123 in MDCK-MDR1 cells. This inhibition on Pgp-mediated efflux was also confirmed by confocal microscopy. In conclusion, BCs could be further developed as polymeric nano-carriers for those drugs with Pgp-mediated efflux.
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JOURNAL OF FOOD ENGINEERING
·
2025
·
IF 5.8
Ying Ren,Dan Chen,Shiyu Lin,Xiaoping Fan,Liming Cao
Chitosan (CS), an abundant and biodegradable natural polymer, represents a highly valuable alternative to non-biodegradable packaging materials. But it is limited by unsatisfactory mechanical, barrier, and antibacterial properties. Food waste is rich in active ingredients. Using them for food preservation not only facilitates a closed-loop of resource utilization but also reduces dependence on traditional preservatives, providing a more eco-friendly and sustainable solution. In this study, nitrogen-doped carbon dots (N-CDs) were synthesized from orange peel waste via a green hydrothermal method. Sustainable multifunctional composite films were prepared by compounding N-CDs with chitosan (CS). The results showed that adding N-CDs enhanced the mechanical properties, UV protection, water barrier, antibacterial activity, and antioxidant capabilities of the composite films. Furthermore, the films also exhibited good humidity-responsive fluorescent sensing capability, making them suitable for smart humidity monitoring. When used for fruit preservation, the composite films could effectively extend the shelf life. This study of multifunctional packaging films composed of seafood waste (CS) and fruit waste (N-CDs) providing an easy approach for the high-value utilization of food waste and the sustainable development of food packaging materials.
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