Advanced Science
·
2025
·
IF 14.1
Xiaoli Li,Sizhao Xing,Yanli Li,Guangshuo Wang,Xiaoyi Gao,Dong Li
Sub-1-nm nanowires (NWs) with fascinating physicochemical properties have demonstrated remarkable potential for applications across various fields. However, it remains a great challenge to prepare sub-1-nm NWs with near-infrared (NIR) absorption characteristics and explore their optoelectronic applications, so far. Herein, a novel cation-exchange strategy in N, N-dimethylformamide (DMF) solvent is introduced to synthesize sub-1-nm PbSe NWs, starting from sub-1-nm ZnSe NWs. Theoretical calculations and nuclear magnetic resonance (NMR) measurements have confirmed that the cation exchange reaction in DMF effectively reduces the barriers for Zn 2+ extraction and Pb 2+ introduction compared to the conventional toluene/methanol system. Notably, the resulting sub-1-nm PbSe NWs exhibit a strong absorption peak at ≈940 nm. Leveraging their unique NIR absorption features and superior carrier transport properties, self-powered photoelectrochemical-type (PEC) photodetectors is fabricated based on sub-1-nm PbSe NWs embedded in polyvinylidene fluoride (PVDF) composite films. These photodetectors demonstrated exceptional photoresponse performance under 940 nm illumination (8.0 mW cm −2 ), with a typical on/off ratio of 4860, a detectivity of 4.65 × 10 11 Jones, and a responsivity of 113 mA W −1 . This work provides a new approach for developing and investigating NIR photoactive sub-1-nm semiconductor NWs with broad application prospects.
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高影响因子
Frontiers in Pharmacology
·
2025
·
IF 4.8
Lili Huang,Yinqi Chen,Hua Zhou,Huihui Chen,Xiping Wu,Zhuochao Wu,Zhoudi Liu,Zhiwei Zheng
Background: Cerebral ischemia-reperfusion injury (CIRI) is a secondary brain injury that occurs after thrombolysis and is a primary cause of death in ischemic stroke patients. Antioxidants that effectively reduce oxidative stress are an efficient treatment approach for CIRI. Here, a novel diimide compound was synthesized using the chemical structure of previously designed anti-inflammatory skeletons.Methods and results: The antioxidant activities of five compounds (Z1–Z5) were preliminarily evaluated using the hydrogen peroxide-induced PC12 cell damage model, of which Z3 exhibited the best antioxidant effect, even exceeding that of the positive control (tert-butylhydroquinone). Enrichment analysis using network targeting and network pharmacology methods predicted seven candidate core target genes of Z3 in CIRI. Of these targets, computer molecular docking analysis predicted that Z3 has the strongest binding affinity for nuclear factor erythroid 2-related factor (Nrf2). MTT and colony formation assays, reactive oxygen species analysis, immunofluorescence, and immunoblotting experiments verified that Z3 reduced reactive oxygen species to play a protective antioxidant role via the Nrf2/hemoxygenase 1 (HO-1) pathway. The protective effect of Z3 in vivo was explored through TTC staining and neurobehavioral scoring of CIRI model mice.Conclusion: This study provides a new drug development strategy and candidate drug for the treatment of CIRI, offering ideas for the design of new antioxidants.
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FREE RADICAL BIOLOGY AND MEDICINE
·
2025
·
IF 8.2
Tongwang Luo,Shengzhe Song,Shujie Wang,Sheng Jiang,Bin Zhou,Quanjiang Song,Lingjun Shen,Xiaodu Wang,Houhui Song,Chunyan Shao
Cadmium (Cd), a pervasive environmental toxicant, poses significant threats to human and animal health through multi-organ toxicity. While ferroptosis has been implicated in Cd-induced pathologies, the molecular mechanisms underlying Cd-mediated nephrotoxicity remain poorly understood. This study elucidates the ferroptosis pathway in CdCl 2 -exposed PK-15 cells and murine kidney, characterized by iron overload, lipid peroxidation, and mitochondrial dysfunction, which were ameliorated by ferroptosis inhibitor ferrostatin-1. Transcriptomic analysis revealed substantial upregulation of heme oxygenase-1 (HO-1) following CdCl 2 exposure. Mechanistically, CdCl 2 triggered nuclear translocation of nuclear factor erythroid 2-related factor-2 (NRF2), subsequently activating HO-1 transcription. Over-activated HO-1 catalyzes the decomposition of heme and releases free iron, accompanied with the degradation of ferritin heavy chain 1 (FTH1) induced by CdCl 2 exposure, which leads to intracellular iron overload and excessive lipid peroxides production through Fenton reaction, resulting in ferroptosis ultimately. In vivo validation confirmed NRF2/HO-1-mediated ferroptosis in CdCl 2 -induced murine nephrotoxicity. Both pre-treatment with HO-1 competitive inhibitor Zinc protoporphyrin IX (ZnPP) and knockout of HO-1 gene remarkably alleviated PK-15 cells against ferroptosis induced by CdCl 2 treatment. Our findings demonstrate that Cd exposure initiates NRF2-mediated HO-1 overexpression, driving iron-dependent lipid peroxidation and subsequent ferroptosis. This mechanistic insight provides potential therapeutic targets for mitigating Cd-induced renal damage, advancing our understanding of heavy metal toxicity and its cellular consequences.
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Food Bioscience
·
2025
·
IF 5.9
Xing Chen,Menglin Jiang,Jie Liu,Genyi Zhang
Whole grain food is a type of healthy food with many beneficial effects, but the fundamental mechanism is still illusive. In the current study, a whole grain component (WGC), as a whole grain model, was prepared by mimicking the endosperm cellular structure through encapsulating glucomannan (dietary fiber) and starch in a matrix formed by polysaccharide gelation. A high level of slowly digestible starch (SDS) (>50 %) in WGC was observed from an in vitro digestion experiment. When the in vivo test of WGC was conducted, a reduced postprandial glycemia and an increased GLP-1 response were observed, which confirmed the stimulation effect of WGC on the secretion GLP-1, an incretin hormone with numerous health benefits. Further study showed that the blood glucose level (from 29.2 to 9.5 mmol/L) and the biomarker HbA1c (from 10.5 to 6.4 %) in diabetic mice were significantly reduced under the condition of WGC-containing diet (WGC-diet). The β-cell function and insulin sensitivity were also significantly improved. Furthermore, the WGC-diet reversed the dysbiosis with increased production of healthy short-chain fatty acids, which are likely the basis to the secretion of GLP-1. Gene expression revealed that WGC-diet activated the GLP-1 signaling pathway, leading to an increased secretion of insulin and improved β-cell function, which all contribute to the glycemic control in diabetic mice. Thus, GLP-1, as pleotropic incretin hormone, could be an important mechanism to the various health benefits of whole grain foods.
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Current Research in Food Science
·
2022
·
IF 6.269
Kwame Eduam Baiden Frempong,Yan Chen,Lili Liang,Xiaoyan Lin
During post-harvest storage, the cell wall properties are closely associated with the physical, chemical, and biological properties of the fruit. The degradation of pectin in the cell walls and middle lamella is critical to these properties. The effects of calcium chloride (CaCl₂) and 1-methylcyclopropene (1-MCP) combined treatment on the pectin degradation, texture, and peel color of Eureka lemon were investigated during post-harvest storage. The in-situ light microscope analysis, rapid method, and FTIR test were used to investigate the spatial distribution, the pectin content, and its degradation. The results showed a reduction in pectin degradation, by 42 d the CaCl₂ and 1-MCP combined treated fruits presented a 36.7% pectin content loss which was lower than the control which was 48.3%. The treated fruits significantly exhibited enhanced textural properties, delayed weight loss, higher total acids, and improvement of other physicochemical properties in comparison to the control. The treatment deaccelerated the fruit peel color change from green to yellow and also had a better visual appearance on the final day. Overall, the results suggest that the control treatment for pectin degradation can reduce the fruit texture decline and peel color change and maintain a good visual appearance. The influence of pectin degradation on the texture and physicochemical properties of lemon provides a theoretical basis for fruit storage optimization, quality control, and shelf-life extension.
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高影响因子
JOURNAL OF STRUCTURAL CHEMISTRY
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2022
·Vol. 63
·期 10
·1610-1615
·
IF 1.004
Wang G. -F.,Sun S. -W.
Abstract Two coordination polymers, {[Co 2 (L 1 ) 2 (adc) 2 ]·5H 2 O} n ( 1 ) and {[Ni(L 1 ) 2 (Hadc) 2 ]} n ( 2 ) (L 1 = bis(4-(1 H -imidazol-1-yl)phenyl)methanone, H 2 adc = adamantane-1,3-dicarboxylic acid), have been synthesized and structurally characterized. In 1 , Co1 is five-coordinated by three oxygen atoms of two adc 2– and two nitrogen atoms from two L 1 ligands. The neighboring [Co(adc)] 2 dimers are linked through four L 1 ligands to form a 1D ladder structure. In 2 , Ni1 is six-coordinated by two oxygen atoms from two Hadc – ligands, four nitrogen atoms from four distinct L 1 ligands to give a slight distorted octahedral geometry. The [Ni(Hadc) 2 ] units are bridged by L 1 ligands to form 1D looped chains.
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ORGANIC PROCESS RESEARCH & DEVELOPMENT
·
2022
·
IF 3.858
Ling He,Wenjie Ye,Youyu Xie,Qinghai Liu,Hualei Wang,Dongzhi Wei
Bioreductions catalyzed by ketoreductases play an important role in the synthesis of chiral alcohols. However, the synthesis of (R)-2-chloro-1-(3,4-difluorophenyl)ethanol (24b), an important chiral intermediate for the synthesis of the anticoagulant ticagrelor, poses significant challenges in terms of high substrate concentration requirements that limit its production. In this study, a novel NADH-dependent, short-chain dehydrogenase, PpKR8, from Paraburkholderia phymatum STM815, exhibited excellent enantioselectivity and high activity for the production of (R)-CFPL (24b) through the reduction of 2-chloro-1-(3,4-difluorophenyl)ethanone (24a). The coexpression of PpKR8 and glucose dehydrogenase from Bacillus subtilis in Escherichia coli allowed up to 300 g/L (1.57 M) CFPO (24a) to be completely converted into 24b with 99.9% enantiomeric excess and a high space–time yield (728 g/(L day)). Substrate specificity assays demonstrated a broad substrate spectrum for PpKR8, which included 35 α/β-ketoesters, aromatic ketones, and heterocyclic ketones. Moreover, three additional optically pure chiral alcohols that are used as important drug intermediates were synthesized at high substrate concentrations (150–330 g/L), demonstrating the excellent industrial potential of PpKR8-mediated bioreductions.
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International Journal of Nanomedicine
·
2022
·
IF 7.033
Wei Shang,Xin Zhao,Fan Yang,Dongyi Wang,Le Lu,Zihan Xu,Zhiming Zhao,Hui Cai,Junyi Shen
Background: Compared with traditional drugs, nanomaterial drugs have the benefits of improving the solubility, bioavailability, and absorption rate of insoluble drugs. Nanoporous complexes can increase the efficiency with which drugs can penetrate the blood-brain barrier and reach target organs. Ginsenoside Rg1 is an effective drug that promotes angiogenesis. Ginsenoside Rg1 composite nanoparticles were employed to induce the expression of several key epigenetic enzymes and then activate the VEGF and Notch pathways after the onset of ischemic brain lesions. Methods: We constructed nanoparticles to fully encapsulate the therapeutic drug (ginsenoside Rg1), which can be transferred into brain tissue via the receptor-mediated transfer of drug-encapsulated nanoparticles. Evaluation of the therapeutic effect of ginsenoside Rg1 complex nanovesicles (CNV) was performed by in vitro and in vivo experiments. Real-time polymerase chain reaction (RT- PCR), Western blot, immunohistochemistry staining (IHC), and Co-immunoprecipitation (co-IP) were employed to screen for epigenetic enzymes with an up-regulated expression post ginsenoside Rg1-CNV intervention. RNA sequencing, shRNA knockdown, and chromatin Immunoprecipitation (ChIP) sequencing were performed to detect the target genes of ginsenoside Rg1-CNV that regulate angiogenesis. Then, bioinformatic analysis was performed to investigate the mechanism of action of epigenetic modifying enzymes in regulating target genes. Results: The average of the synthesized ginsenoside Rg1-CNV was 203.78±6.83 nm, the polydispersion index was 0.135±0.007, and the Zeta potential was 23.13±1.65 mV. Through in vivo and in vitro experiments, we found that it promotes the proliferation, migration, and tubular formation of brain microvascular endothelial cells (BMECs). Meanwhile, the intervention of ginsenoside Rg1-CNV promoted the demethylation of H3K27me3 within the promoter region of VEGF-A and Jagged1 genes and reduced the H3K27me3 modification within this region. Conclusion: The ginsenoside Rg1 nanoparticles may be an available blood-brain barrier penetrating agent for ischemic stroke.
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CHEMICAL ENGINEERING JOURNAL
·
2014
·
IF 4.058
Zhi-yun Kong,Jun-fu Wei,Yong-hua Li,Na-na Liu,Huan Zhang,Yue Zhang,Li Cui
The quaternary ammonium fibers which could remove Cr(VI) ions rapidly were prepared by radiation-induced grafting of 2-(dimethylamino)ethyl methacrylate (DMAEMA) onto polypropylene (PP) fibers (PP-g-DMAEMA) and modifying with 1-bromoalkanes. The FTIR, FESEM, XPS and TG-DTG spectra manifested that the quaternary ammonium group was introduced onto the surface of PP fibers. The main factors influencing the adsorption of Cr(VI) ions including contact time, pH of the aqueous solution and adsorption dosage were investigated. The removal rate could reach 97% within 4 min and the kinetics study indicated that adsorption process was surface reaction controlled. Maximum adsorption capacities of Cr(VI) ions at 25 °C were 105.8 mg/g based on the Langmuir isotherms. These results suggested that the quaternary ammonium fibers have potential application for the removal of hazardous metal pollutants from wastewater highly and rapidly.
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高影响因子
NEUROREPORT
·
2020
·Vol. 31
·期 18
·1274-1282
·
IF 1.394
Xi Zhiyu,Chen Xiao,Xu Canxin,Wang Baofeng,Zhong Zhihong,Sun Qingfang,Sun Yuhao,Bian Liuguan
The brain edema following intracerebral hemorrhage (ICH) plays a key role in the recovery process. Protocatechuic acid (PCA) has been proved possessing neuroprotection in ICH. Here we tried to explore its value in brain edema after ICH and reveal underlying mechanisms. ICH model was
created in C57 mice using collagenase IV. PCA was injected intraperitoneally at 30 mg/kg every 24 h in PCA group. On day 3 after ICH, the water content of hemorrhagic ipsilateral hemisphere in PCA group was significantly reduced compared with vehicle group. AQP4, the main water channel, was
remarkably decreased in PCA group. Additionally, ZO-1 and occludin expression were increased in PCA group. The bEnd.3 cells were cultured to understand the effect of PCA on the blood-brain barrier (BBB) integrity. Compare to hemin treated group, plus PCA enhanced the expression of HO-1 and
Nrf2 nuclear translocation. Furtherly, the overexpression of HO-1, ZO-1, occludin, in the PCA treatment group was inhibited after knockdown of Nrf2. Taken together, our results proved PCA alleviated brain edema and BBB disruption in ICH by promoting the Nrf2/HO-1 signaling pathway.
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Journal of Integrative Agriculture
·
2023
·
IF 4.8
Ke-da SHI,Yan LI,Min-sheng XU,Kun-li ZHANG,Hong-chao GOU,Shao-lun ZHAI,Chun-ling LI
Streptococcus suis serotype 2 ( S. suis 2) is a zoonotic pathogen that clinically causes severe swine and human infections (such as meningitis, endocarditis, and septicemia). In order to cause widespread diseases in different organs, S. suis 2 must colonize the host, break the blood barrier, and cause exaggerated inflammation. In the last few years, most studies have focused on a single virulence factor and its influences on the host. Membrane vesicles (MVs) can be actively secreted into the extracellular environment contributing to bacteria-host interactions. Gram-negative bacteria-derived outer membrane vesicles (OMVs) were recently shown to activate host caspase-11-mediated non-canonical inflammasome pathway via deliverance of OMV-bound lipopolysaccharide (LPS), causing host cell pyroptosis. However, little is known about the effect of the MVs from S. suis 2 (Gram-positive bacteria without LPS) on cell pyroptosis. Thus, we investigated the molecular mechanism by which S. suis 2 MVs participate in endothelial cell pyroptosis. In this study, we used proteomics, electron scanning microscopy, fluorescence microscope, western blotting, and bioassays, to investigate the MVs secreted by S. suis 2. First, we demonstrated that S. suis 2 secreted MVs with an average diameter of 72.04 nm, and 200 proteins in MVs were identified. Then, we showed that MVs were transported to cells via and dynamin-dependent endocytosis. The S. suis 2 MVs activated NLRP3/Caspase-1/GSDMD canonical inflammasome signaling pathway, resulting in cell pyroptosis, but it did not activate the caspase-4/-5 pathway. More importantly, endothelial cells produce large amounts of reactive oxygen species (ROS) and lost their mitochondrial membrane potential under induction by S. suis 2 MVs. The results in this study suggest for the first time that MVs from S. suis 2 were internalized by endothelial cells via mainly dynamin-dependent endocytosis and might promote NLRP3/caspase-1/GSDMD pathway by mitochondrial damage, which under induction produced mtDNA and ROS, leading to the pyroptosis of endothelial cells.
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SOLAR ENERGY
·
2018
·
IF 4.374
Xichuan Yang,Weihan Wang,Yanyun Zhang,Licheng Sun
P-type doping is a rational strategy for the enhancement of hole transporting properties of the organic semiconductors as well as the device performance of organic photo-electric devices. We originally introduce a stable and solution processed p-type dopant based on N-oxoammonium salts termed 2,2,6,6-tetramethyl-1-oxopiperidinebromide salt (TEMPO-Br) for 2,2′,7,7′-tetrakis(N, N-di-p-methoxyphenyl-amine)9,9′-spirobifluorene (Spiro-OMeTAD) based solid state dye sensitized solar cells (ssDSC). By introducing TEMPO-Br doped Spiro-OMeTAD and a commercialized D-π-A sensitizer into ssDSCs, a promising device performance of 6.83% is achieved under simulated AM 1.5G solar irradiation (100 mW cm −2 ), which is significantly better than the control devices (∼1.7 times). The doping effect of TEMPO-Br on the photophysical and electrochemical properties of Spiro-OMeTAD, solid state device performance, cationic dye regeneration kinetics and preliminary long-term stability have been systematically investigated. This work indicates a potential application of N-oxoammonium salts as chemical P-type dopants for triphenylamine based hole transporting materials in solid state photo-electric devices.
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