JOURNAL OF CHEMICAL AND ENGINEERING DATA
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2025
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IF 2.1
Dan Li,Xiazhen Zhang,Xue Meng,Longxia Hao,Yixin Li,Guoqing Sui
The density and dynamic viscosity of the ternary system (dimethyl carbonate + methyl myristate + 1-hexanol) and the related binary mixtures (dimethyl carbonate + methyl myristate), (dimethyl carbonate + 1-hexanol), and (methyl myristate + 1-hexanol) were experimentally determined in the temperature range T = 293.15–323.15 K, which were applied to obtain the excess molar volume (VmE) and deviation in viscosity (Δη) of ternary and its related binary systems. Redlich–Kister, Cibulka, and Singh equations were used to estimate VmE and Δη of ternary mixtures. The VmE consistently exhibited positive values for all studied systems, while the Δη values were negative, which provided data support for the thermodynamic study and application of the biodiesel mixed fuel system.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2024
·
IF 6.2
Huiqian Chen,Sailing Chen,Yunbing Tang,Yingfen Ying,Shaowei Wang,Yang Zhu,Yiyan Wang,Ren-shan Ge,Ping Duan
Dicarboximide fungicides, including captafol, captan, cyclohexylthiophthalimide, folpet, and procymidone, represent a distinct category of fungicides. 17β-Hydroxysteroid dehydrogenase 1 (17β-HSD1) catalyzes the conversion of estrone to estradiol in mammals. Yet, the impact of these fungicides on 17β-HSD1 activity remains unknown. In this study, we investigated their inhibition using human placental cytosols, rat and pig ovarian cytosols. Our observations revealed that dicarboximide fungicides significantly inhibited human 17β-HSD1 activity. Among them, captan showed the strongest potency, with its IC 50 of 1.28 μM, whereas procymidone had an IC 50 of 100.71 μM. However, both rat and pig 17β-HSD1 enzymes were less sensitive to the inhibition of these fungicides compared to the human enzyme, with captan displaying an IC 50 of 5.65 μM for the rat enzyme and 7.36 μM for the pig enzyme. Correlation analysis indicated a positive correlation between IC 50 values and LogP. Docking analysis revealed that these fungicides bound to cofactor or between the steroid and cofactor binding sites. The dithiothreitol treatment demonstrated that the formation of irreversible bonds between dicarboximide fungicides and the cysteine residues played a key role in the inhibition of 17β-HSD1 activity. In conclusion, dicarboximide fungicides inhibit 17β-HSD1 depending on lipophilicity, species, and cysteine residue interactions.
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高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2025
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IF 9.7
Xianqiang Yu,Linfeng Zhang,Ruikai Qi,Mingze Xia,Yuezhu Wang,Mengxiao Zhong,Wei Song,Xiaofeng Lu
Ruthenium dioxide (RuO 2 ) stands out as a versatile catalyst for acidic oxygen evolution reactions (OER), yet it falls short in alkaline conditions, and its hydrogen evolution reaction (HER) activity is generally lacking. This poses a significant challenge in engineering a robust bifunctional electrocatalyst capable of excelling in both alkaline HER and OER through manipulating the electronic structure of RuO 2 . Here, we have developed a nanofibrous rhenium (Re)-doped RuO 2 electrocatalyst via an electrospinning-calcination process, tailored for comprehensive water splitting applications. By finely tuning the Re doping content, the optimized Re-doped RuO 2 catalyst achieves overpotentials of merely 386 mV for OER and 157 mV for HER at a current density of 1 A cm −2 in 1 M KOH, significantly outperforming the conventional RuO 2 and Pt/C catalysts in their respective reactions. This bifunctional Re-doped RuO 2 catalyst showcases unparalleled efficiency in overall water splitting, demanding an impressively low power consumption of 6.36 kWh m −3 H 2 at the elevated current density of 1 A cm −2 , which substantially surpasses the performance of the benchmark Pt/C||RuO 2 system. Moreover, it maintains excellent stability under such demanding high current density conditions. Our research provides profound insights into the design of highly efficient RuO 2 -based catalysts, paving the way for their deployment in practical industrial water electrolysis technologies
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ACS Applied Materials & Interfaces
·
2015
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IF 6.723
Hailei Zhang,Tianfei Ren,Yunjing Ji,Lingui Han,Yonggang Wu,Hongzan Song,Libin Bai,Xinwu Ba
A novel fluorescence probe based on modified halloysite nanotubes (HNTs) by using 1-pyrenylboronic acid selectively grafted onto the inner surface of lumen was successfully achieved. The solid-state nuclear magnetic resonance (13C and 11B), X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR) confirmed that the boronic acid group only binds to alumina at the tube lumen and does not bind the tube’s outer siloxane surface. The modified HNTs (HNTs-PY) inherit the spectroscopic properties relating to the pyrene units. Interestingly, the established Al–O–B linkage gives the H2O2-sensitivity to pyrene grafted tubes. HNTs-PY exhibits a highly specific “turn-off” response for hyperoxide over other reactive oxygen species (ROS) and oxidative ions owing to their chemoselective boronate-to-phenol switch. The “turn-off” response can even be tracked when the additional amount of H2O2 was limited to 1 × 10–6 mol. Thus, the selective modification method under mild conditions for the design of novel organic–inorganic hybrid fluorescence probe may open up a broader application as well as for identification and diagnosis.
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Oxidative Medicine and Cellular Longevity
·
2022
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IF 7.31
Shiqi Gao,Liuzhi Zhou,Jianan Lu,Yuanjian Fang,Haijian Wu,Weilin Xu,Yuanbo Pan,Junjie Wang,Xiaoyu Wang,Jianmin Zhang,Anwen Shao
Background. Ferroptosis is a newly identified form of programmed cell death caused by iron-dependent lipid peroxidation. Our study was designed to determine the expression patterns and role of 15-lipoxygenase-1 (ALOX15) in subarachnoid hemorrhage (SAH) and to investigate whether cepharanthine (CEP) can inhibit ferroptosis by inhibiting ALOX15 in specific cell types. Methods. A mouse model of SAH was developed by the endovascular perforation method. bEend.3 endothelial cells and BV2 microglial cells as well as RSL3 and hemin were used to simulate SAH in vitro. Mice and cell lines were treated with CEP and a group of specific oxygenase inhibitors to explore the protection effect from ferroptosis. Lipid peroxidation staining with BODIPY 581/591 C11 and transmission electron microscopy were used to identify ferroptosis in vitro and in vivo. Results. In the present study, the accumulation of lipid peroxide, a defect in the glutathione peroxidase 4 (GPx4)/glutathione (GSH) antioxidant system, highly expressed ALOX15 in microglia and endothelium, and ferroptotic changes in microglial mitochondria confirmed the occurrence of ferroptosis after SAH in vivo. Further, CEP was shown to inhibit ferroptosis and improve neurological function by downregulating the expression of ALOX15. During in vitro experiments, we investigated the important role ALOX15 in RSL3-induced endothelial ferroptosis. In addition, we found that M2-type microglia are more sensitive to RSL3-induced ferroptosis than M1-type microglia and that hemin probably induced ferroptosis in M2-type microglia by increasing ALOX15 levels and decreasing GPx4 levels. The effect of CEP treatment was also demonstrated in vitro. Conclusions. In summary, to the best of our knowledge, this is the first study demonstrating that ferroptosis occurred in the microglia and endothelium after SAH, and this process was facilitated by increased ALOX15 levels. More importantly, treatment with CEP could inhibit ferroptosis through downregulating the expression of ALOX15.
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Journal of Energy Chemistry
·
2025
·
IF 14.9
Jingchi Zhang,Ze Hua,Ziqi Wu,Xinting Cao,Wen Yang,Ruiwen Shao,Yu Bai,Zhenhua Wang,Kening Sun
To satisfy the demands of modern society for high-energy–density sulfide-based all-solid-state lithium batteries (ASSLBs), Ni-rich cathode materials have gained much attention for their high capacity and energy density. However, their practical deployment is hindered by accelerated interface degradation and capacity decay originating from surface oxygen release and lattice oxygen activation during prolonged cycling. In this study, Ti x NbB (1− x ) C 2 was successfully coated on the surface of LiNi 0.94 Co 0.05 Mn 0.01 O 2 . Density functional theory (DFT) calculations first elucidate a “point-to-point” anchoring mechanism where each surface oxygen atom coordinates with single species (Ti/Nb/B) offered by Ti x NbB (1− x ) C 2 , which forms robust O–M bonds and sustain a stable interface structure. The electron energy loss spectroscopy (EELS) reveals the segregation of Ti/Nb toward subsurface layers during cycling, creating an optimized lattice oxygen coordination environment and suppressing oxygen activation. The dual oxygen stabilization mechanism dramatically improves the reversibility of phase transition and the structural stability of the Ni-rich cathode materials. Moreover, Ti x NbB (1− x ) C 2 as the protective layer decreases mechanical strain and suppresses the parasitic reactions. Consequently, the engineered cathode delivers 91% capacity retention after 1000 cycles at 0.3 C, suggesting excellent cycling stability. The research delivers a new design philosophy for the coating layer that can stabilize surface oxygen. Furthermore, the atomistic understanding of the structure–property relationship of the Ni-rich cathode materials provides valuable guidance for the future design of new cathode materials with superior structural stability in ASSLBs.
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International Journal of Biological Sciences
·
2020
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IF 4.858
Yiliang Wang,Lianzhou Huang,Yun Wang,Weisheng Luo,Feng Li,Ji Xiao,Shurong Qin,Zhaoyang Wang,Xiaowei Song,Yuan Wang,Fujun Jin,Yifei Wang
Herpes simplex virus (HSV) type 1 (HSV-1) infection exhibited high heterogeneity at individual cells level, including the different gene expression patterns and varying amounts of progeny virus. However, the underlying mechanism of such variability remains obscure. The importance of host long noncoding RNAs (lncRNAs) in virus infection had been recognized, while the contribution of lncRNAs to the heterogeneous infection remains unknown. Herein, a prior single-cell RNA sequencing data using HSV-1 reporter strain expressing ICP4-YFP was re-analyzed to obtain the differentially expressed lncRNA between the successfully initiated viral gene expression (ICP4-YFP + ) cells and the aborted infection cells (ICP4-YFP - ). The ICP4-YFP + population show a higher abundance of MAMDC2 antisense 1 (MAMDC2-AS1) lncRNA than ICP4-YFP - population. MAMDC2-AS1 silencing reduces the expression of HSV-1 immediate early (IE) genes and limit HSV-1 infection in human host cells. Consistently, ectopic expression of MAMDC2-AS1 enhances HSV-1 IE genes transcription and facilitates the formation of HSV-1-induced plaques. Mechanically, both RNA-pull down and RNA immunoprecipitation assays show that MAMDC2-AS1 interacts with the RNA binding protein heat shock protein 90α (Hsp90α), a molecular chaperone involving in the nuclear import of HSV-1. The MAMDC2-AS1-Hsp90α interaction facilitates the nuclear transport of viral tegument protein VP16, the core factor initiating the expression of HSV-1 IE genes. The transcription factor YY1 mediates the induction of MAMDC2-AS1 upon HSV-1 infection. Our study elucidates the contribution of lncRNA to HSV-1 infection susceptibility in human cells and the role of Hsp90α RNA binding activity in HSV-1 infection.
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Autophagy
·
2018
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IF 11.1
Zhen Yang,Qi Sun,Junfei Guo,Shixing Wang,Ge Song,Weiying Liu,Min Liu,Hua Tang
Emerging evidence has revealed that miRNAs could upregulate the expression levels of target genes. However, the molecular mechanism underlying upregulation of targets mediated by miRNAs remains unclear. In this study, we found a novel miRNA named MIR-G-1 by GRSF1-RNA immunoprecipitation (RIP)-deep sequencing, which could directly target and upregulate LMNB1 and TMED5 in a GRSF1-dependent manner in cervical cancer cells. In addition, upregulated MIR-G-1 in cervical cancer promoted a malignant phenotype in vitro and in vivo. TMED5 could interact with WNT7B and thus activated the canonical WNT-CTNNB1/β-catenin signaling pathway. MIR-G-1 mediated the activation of this pathway. Furthermore, MIR-G-1 promoted serum starvation-induced nuclear macroautophagy/autophagy, and accelerated taxol (TAX)-induced DNA-damage repair in cervical cancer cells. Collectively, these findings may provide a new insight into the upregulation mechanism and nuclear autophagy mediated by miRNAs and provide a potential biomarker for cervical cancer.Abbreviations: 3ʹUTR: 3′ untranslated region; EMSA: electrophoretic mobility shift assay; EMT: epithelial-mesenchymal transition; GRSF1: G-rich RNA sequence binding factor 1; IF: immunofluorescence; IP: immunoprecipitation; IHC: immunohistochemistry; lnc: long noncoding; miRNA:microRNA; TAX: taxol; TMED5: transmembrane p24 trafficking protein 5
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高影响因子
CELL CYCLE
·
2021
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IF 4.534
Yanming Yu,Daoxu Wu,Yongwei Li,Hui Qiao,Zhengfei Shan
Background Ketamine-related cystitis (KC) has been researched in many clinical studies, but its exact mechanism is ambiguous and needs further research. Methods We established a KC rat model and analyzed physiological, biochemical, and urodynamic parameters of ketamine (KET)-related bladder injury. Bladder histologic feature, reactive oxygen species (ROS), autophagy-, apoptosis-, and endoplasmic reticulum stress (ERS)-related markers were examined by hematoxylin and eosin staining, Masson staining, ROS kit, quantitative real-time polymerase chain reaction, and western blot. In vitro, effects of 0.01, 0.1, and 1 mM KET on cell vitality, apoptosis, ROS level, autophagy-, apoptosis-, and ERS-related markers were examined again. Effects of KET-1 and salubrinal on complex formation, autophagy-, apoptosis-, and ERS-related markers were examined by Co-Immunoprecipitation and western blot. After transfection with shIRE1, complex formation, cell biological behaviors, ROS level, autophagy-, apoptosis-, and ERS-related markers were examined again. Results KET induced bladder hyperactivity and injury. KET facilitated urinary frequency, ROS production, and induced bladder histologic injury by activating autophagy-, apoptosis-, and ERS-related markers in rats. In vitro, KET (0.01, 0.1, and 1 mM) restrained cell vitality and elevated apoptosis and ROS level via activating autophagy-, apoptosis-, and ERS-related markers. Moreover, salubrinal reversed the promotion of KET-1 on complex formation, autophagy-, apoptosis-, and ERS-related marker expressions. After transfection with shIRE1, shIRE1 weakened complex formation induced by KET-1, and the effects of KET-1 on cells were offset by shIRE1. Conclusion KET enhanced autophagy and ERS in vivo and in vitro via restraining IRE1-TRAF2-ASK1-JNK pathway.
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高影响因子
Journal of Materials Chemistry B
·
2022
·Vol. 10
·期 41
·8535-8548
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IF 7.571
Jiaxin Wu,Shaokang Jiang,Wenjia Xie,Yiyuan Xue,Mingxin Qiao,Xiangjun Yang,Xin Zhang,Qianbing Wan,Jian Wang,Junyu Chen,Xibo Pei
Biocompatibility and osteointegration of implants are highly desired in orthopedic and dentistry applications. The synthesis of a coating with ideal biocompatibility and osteogenic effect carries practical significance for improving the bio-inertness of pure Ti implants. Metal–organic frameworks (MOFs) are effective surface modification agents in bone regeneration applications. Bio-MOF-1, a classic type of biofriendly MOF with a bio-derived constitution, possesses biocompatibility and osteogenic potential resulting from its Zn core and adenine ligand. In this study, bio-MOF-1 coatings at multiple concentrations were synthesized on alkali-heat treated Ti, and their cytocompatibility and osteogenic properties were systematically examined both in vitro and in vivo. Coatings were characterized to confirm the successful synthesis of bio-MOF-1 coatings. These coatings exhibited advanced thermostability, excellent biocompatibility, and stable Zn2+ release, which up-regulated the expression of osteogenesis-related genes and proteins. Furthermore, bio-MOF-1 coating of Ti implants enhanced early osseointegration at the bone–implant interface. This study demonstrates the promising potential of bio-MOF-1 coatings with the osteogenic effect for surface modification in bone tissue engineering.
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高影响因子
Plants-Basel
·
2025
·Vol. 14
·期 24
·3718
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IF 4.1
Xinxin Li,Ziting Zhu,Jing Wu,Nianjun Yu,Huiqun Xie,Lan Han,Daiyin Peng
Dendrobium huoshanense , a rare and endangered medicinal orchid species endemic to Huoshan County, Anhui Province, faces a severe limit of wild resources. Its medicinal efficacy derives from unique chemical constituents, which vary significantly across cultivation modes. We systematically compared sensory properties and nutritional constituents (polysaccharides, flavonoids, alkaloids, bibenzyls, minerals, and free amino acids) between D. huoshanense in three cultivation modes—greenhouse cultivation, understory gravel cultivation, and wild-simulated cultivation. Additionally, the gastroprotective effects of wild-simulated cultivated D. huoshanense on gastric mucosal epithelial cells were evaluated. Results demonstrated that wild-simulated cultivation yielded significantly higher levels of nutritional constituents compared to gravel and greenhouse cultivation. Furthermore, wild-simulated cultivated D. huoshanense exhibited notable protective effects against gastric mucosal epithelial cell damage. This study not only elucidates the influence of cultivation modes on the chemical profile of D. huoshanense but also provides scientific evidence supporting its gastric protective properties, offering a foundation for its sustainable utilization and quality-oriented cultivation.
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Applied Sciences-Basel
·
2025
·Vol. 15
·期 7
·
IF 2.5
Tianyu Hu,Bingying Liu,Siqian He,Yuanjie Teng,Zaifa Pan
Insulin-like growth factor-1 (IGF-1) is a regulatory factor closely associated with diabetes, obesity, and breast cancer, and it also acts as one of the most abundant growth factors in bovine colostrum. Current methods generally have the problem of low sensitivity, a time-consuming nature, and low stability, which makes it difficult to crack down on the false advertising of IGF-1 content in dairy products. In this work, an ultrasensitive fluorescent enzyme-linked immunosorbent assay (ELISA) is proposed, where the antibody and the target are combined in the form of a “sandwich” to ensure the accuracy and specificity of the assay. IGF-1 is quantified based on an effective hydrogen peroxide (H2O2) probe with 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) as the fluorogenic substrate. The proposed fluorescent sandwich ELISA has a low limit of detection (LOD) of 77.29 pg/mL, fast experimental process within 1 h, and stable signal of 1 h. Furthermore, multi-step pretreatment methods for bovine colostrum powders are established to remove interfering substances, including fat, casein, and binding proteins, achieving the accurate and specific detection of IGF-1. IGF-1 recovery studies on treated bovine colostrum powders exhibit good recovery rates ranging from 91.71% to 102.32%, which proves the feasibility of detecting IGF-1 in real bovine colostrum.
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高影响因子
危险品化学品经营许可证（带存储）