Journal of Neuroinflammation
·
2023
·Vol. 20
·期 1
·1-18
·
IF 9.3
Yao Ke,Mou Qianxue,Lou Xiaotong,Ye Meng,Zhao Bowen,Hu Yuanyuan,Luo Jing,Zhang Hong,Li Xing,Zhao Yin
Background Optic nerve injury (ONI) is a key cause of irreversible blindness and triggers retinal ganglion cells (RGCs) change and synapse loss. Microglia is the resistant immune cell in brain and retina and has been demonstrated to be highly related with neuron and synapse injury. However, the function of Sirtuin 1 (SIRT1), a neuroprotective molecule, in mediating microglial activation, retinal synapse loss and subsequent retinal ganglion cells death in optic nerve injury model as well as the regulatory mechanism remain unclear.MethodTo this end, optic nerve crush (ONC) model was conducted to mimic optic nerve injury. Resveratrol and EX527, highly specific activator and inhibitor of SIRT1, respectively, were used to explore the function of SIRT1 in vivo and vitro. Cx3Cr1-CreERT2/RaptorF/F mice were used to delete Raptor for inhibiting mammalian target of rapamycin complex 1 (mTORC1) activity in microglia. HEK293 and BV2 cells were transfected with plasmids to explore the regulatory mechanism of SIRT1.Results We discovered that microglial activation and synapse loss in retinal inner plexiform layer (IPL) occurred after optic nerve crush, with later-development retinal ganglion cells death. SIRT1 activation induced by resveratrol inhibited microglial activation and attenuated synapse loss and retinal ganglion cells injury. After injury, microglial phagocytosed synapse and SIRT1 inhibited this process to protect synapse and retinal ganglion cells. Moreover, SIRT1 exhibited neuron protective effects via activating tuberous sclerosis complex 2 (TSC2) through deacetylation, and enhancing the inhibition effect of tuberous sclerosis complex 2 on mammalian target of rapamycin complex 1 activity.Conclusion Our research provides novel insights into microglial SIRT1 in optic nerve injury and suggests a potential strategy for neuroprotective treatment of optic nerve injury disease.
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高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2023
·Vol. 58
·期 34
·13762-13773
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IF 4.5
Yu Xinrong,Ma Yihang,Ma Zekun,Lian He,Meng Zhaoxu
Cuttlebone as a hemostatic traditional Chinese medicine is usually applied in powder directly to the wound for hemostasis. In order to improve the hemostatic utility of cuttlebone, gelatin/cuttlebone (Gel/Cb) membranes with different Gel/Cb ratios were fabricated through electrospinning and characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, and X-ray diffraction. The hemostatic effect and coagulation process of Gel/Cb electrospun membranes were evaluated in vitro by dynamic whole-blood coagulation test, in vitro hemolysis test, determination of clotting time, and platelet adhesion test and in vivo by liver and tail vein hemostasis models. It was noted that the addition of cuttlebone could significantly decrease the hemolysis rates of Gel/Cb electrospun membranes, which was only about one-sixth of the Gel electrospun membrane. The animal experiments demonstrated effective reduction in bleeding time and volume in Gel/Cb-treated mice. Specially, compared with the commercially available gauze group. Gel/Cb (7:3) electrospun membrane reduced the clotting time by 80.92% and the average bleeding volume by 50.83%. The results further indicated that Gel/Cb electrospun membranes could accelerate coagulation, possibly via the coagulation pathway activation to stimulate platelet contraction and rapid aggregation at the wound surface, providing a new method for rapid hemostasis of acute trauma. Graphical Abstract
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Science China-Materials
·
2023
·Vol. 66
·期 10
·4079-4089
·
IF 8.1
Feng Jie,Kong Fei,Yue Wan-Sheng,Yu Hao,He Zi-Liang,Zhai Ya-Nan,Dong Yu-Bin
Due to the limited concentration of hydrogen peroxide (H 2 O 2 ) in the tumor microenvironment, peroxidase (POD)-mimicking nanozyme-mediated catalytic therapy usually cannot completely eliminate tumor issues. In this study, we report an H 2 O 2 -responsive nanozyme constructed by loading 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) into POD-like Fe-porphyrin covalent organic frameworks (COFs) (Fe-DhaTph). The resulting ABTS@Fe-DhaTph can function as a multifunctional therapeutic nanoagent for combination therapy of catalytic therapy, photothermal therapy (PTT), and photodynamic therapy (PDT). In the tumor microenvironment, ABTS@Fe-DhaTph can catalyze the decomposition of endogenous H 2 O 2 to produce highly cytotoxic hydroxyl radicals (·OH) for in situ tumor catalytic therapy. Meanwhile, the loaded ABTS is oxidized by H 2 O 2 with the assistance of ABTS@Fe-DhaTph, and the resulting oxABTS, with strong near-infrared absorbance, can enable the H 2 O 2 -dependent PTT. Besides Fe-DhaTph-induced PDT, the generated oxABTS with glutathione (GSH)-depletion ability can synergistically enhance the generation of reactive oxygen species, thereby improving catalytic therapy and PDT. To the best of our knowledge, ABTS@Fe-DhaTph is the first COF-based nanozyme to serve as a cascade-amplified synergistic therapeutic nanoagent for cancer treatment.
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JOURNAL OF FLUORESCENCE
·
2023
·
IF 2.7
Heng Chendi,He Bowen,Wang Li
In this study, a dual-mode ratiometric fluorometric and colorimetric platform for the determination of nitrite in pickles was proposed by exquisitely employing the fact that non-fluorescent o -Phenylenediamine (OPD) was oxidized by nitrite under acidic conditions to form fluorescent 2,3-diaminophenazine (DAP) (Em = 575), which meanwhile quench the fluorescent nitrogen-doped carbon dots (N-CDs) at 455 nm, the ratio of fluorescence intensity of DAP to N-CDs (F 575 /F 455 ) changed with the increase of nitrite accompanied by visible color changes. Thus, nitrite can be quantitatively detected within a wide linear range (10–500 µM) with a low detection limit of 0.45 µM due to the high quantum yield of 39.7% of N-CDs. In addition, the colour of the N-CDs/OPD system changed from transparent to yellow when the nitrite was introduced, enabling colorimetric and on-site visual detection. The detection limit of the colorimetric method was 3.03 µM with a linear range of 10–500 µM. The proposed ratiometric fluorometric method has pleasant selectivity and good immunity to interference.
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CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2023
·Vol. 39
·期 5
·790-796
·
IF 3.1
Khan Munir Ullah,Khan Safir Ullah,Cao Xiaohan,Usman Muhammad,Shah Muhammad Naeem,Ghaffar Abdul,Hassan Muhammad,Zhang Chengjian,Zhang Xinghong
I n this study, we report that Berlin Green (FeFe-BG) framework exhibits superior performance in the catalytic coupling of carbon disulfide (CS 2 ) and propylene oxide (PO) to generate a random copolymer containing thioether, propylene monothiocarbonate and ether units. Oxygen and sulfur atom exchange was detected in polymeric and cyclic thiocarbonate byproducts and utilized to modulate the copolymerization of CS 2 and propylene oxide. The coupling of PO and CS 2 was selective for copolymer formation under various reaction conditions. 1 H and 13 C NMR spectroscopy determined two distinct polymer linkages and two cyclic byproducts. Copolymer number average molecular weights ranged from 6.4 kg/mol to 10.5 kg/mol, with a comparatively low polydispersity of 1.3–1.7. The CS 2 /PO molar feed ratio had a significant impact on the O/S exchange process; the ratio of cyclic thiocarbonate byproducts could be efficiently regulated by tuning the CS 2 molar feed ratio.
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NATURE
·
2023
·
IF 64.8
Ni Chujun,Chen Di,Yin Yu,Wen Xin,Chen Xiaolan,Yang Chen,Chen Guancong,Sun Zhuo,Wen Jihang,Jiao Yurong,Wang Chunyang,Wang Ning,Kong Xiangxing,Deng Shihong,Shen Youqing,Xiao Rui,Jin Xiuming,Li Jun,Kong Xueqian,Zhao Qian,Xie Tao
Stimulus-responsive shape-shifting polymers 1 , 2 , 3 have shown unique promise in emerging applications, including soft robotics 4 , 5 , 6 , 7 , medical devices 8 , aerospace structures 9 and flexible electronics 10 . Their externally triggered shape-shifting behaviour offers on-demand controllability essential for many device applications. Ironically, accessing external triggers (for example, heating or light) under realistic scenarios has become the greatest bottleneck in demanding applications such as implantable medical devices 8 . Certain shape-shifting polymers rely on naturally present stimuli (for example, human body temperature for implantable devices) 8 as triggers. Although they forgo the need for external stimulation, the ability to control recovery onset is also lost. Naturally triggered, yet actively controllable, shape-shifting behaviour is highly desirable but these two attributes are conflicting. Here we achieved this goal with a four-dimensional printable shape memory hydrogel that operates via phase separation, with its shape-shifting kinetics dominated by internal mass diffusion rather than by heat transport used for common shape memory polymers 8 , 9 , 10 , 11 . This hydrogel can undergo shape transformation at natural ambient temperature, critically with a recovery onset delay. This delay is programmable by altering the degree of phase separation during device programming, which offers a unique mechanism for shape-shifting control. Our naturally triggered shape memory polymer with a tunable recovery onset markedly lowers the barrier for device implementation.
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Science China-Materials
·
2023
·Vol. 66
·期 10
·4090-4099
·
IF 8.1
Yang Kaidong,Yang Yating,Sun Dongquan,Li Shihua,Song Xiaorong,Yang Huanghao
X-ray nanoscintillator-based photoresponsive therapy is emerging as a promising strategy to treat in vivo tumors, necessitating the development of nanoscintillators with X-ray-excited luminescence in the ultraviolet region (UV-XEL) for the photoactivation of therapeutic precursors. However, it remains a challenge to achieve high-efficiency X-ray-activated tumor therapy due to the limited UV-XEL efficacy. Herein, we developed novel Gd 3+ /Ce 3+ -codoped LiLuF 4 nanoscintillators with strong UV-XEL for in vivo tumor therapy. Significantly, through optimization of host materials, dopants, and energy transfer process, the developed nanoscintillators enabled effective enhancement of UV-XEL (up to 18-fold) as compared with the traditional Ce 3+ -doped counterparts. As a proof-of-concept study to evaluate the in vivo therapeutic applications of the nanoscintillators, we integrated them with nitric oxide (NO) precursors for controllably generating NO upon the X-ray exposure, achieving superior in vivo antitumor effect upon X-ray-induced synergetic NO therapy and radiotherapy. Moreover, X-ray-activated NO therapy can inhibit tumor metastasis into liver to suppress tumor regrowth and prolong mice survival. This work might motivate future development of X-ray nanoscintillators for treating diseases in deep tissue.
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Nature Ecology & Evolution
·
2023
·Vol. 7
·期 11
·1892-1902
·
IF 16.8
Ju Yun,Zhang Hong,Jiang Yanxiao,Wang Wenxin,Kan Guangfeng,Yu Kai,Wang Xiaofei,Liu Jilin,Jiang Jie
The reverse tricarboxylic acid cycle (rTCA) is a central anabolic network that uses carbon dioxide (CO 2 ) and may have provided complex carbon substrates for life before the advent of RNA or enzymes. However, non-enzymatic promotion of the rTCA cycle, in particular carbon fixation, remains challenging, even with primordial metal catalysis. Here, we report that the fixation of CO 2 by reductive carboxylation of succinate and α-ketoglutarate was achieved in aqueous microdroplets under ambient conditions without the use of catalysts. Under identical conditions, the aqueous microdroplets also facilitated the sequences in the rTCA cycle, including reduction, hydration, dehydration and retro-aldol cleavage and linked with the glyoxylate cycle. These reactions of the rTCA cycle were compatible with the aqueous microdroplets, as demonstrated with two-reaction and four-reaction sequences. A higher selectivity giving higher product yields was also observed. Our results suggest that the microdroplets provide an energetically favourable microenvironment and facilitate a non-enzymatic version of the rTCA cycle in prebiotic carbon anabolism.
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NATURE
·
2021
·Vol. 591
·期 7849
·240-245
·
IF 49.962
Shi Xiang,Zuo Yong,Zhai Peng,Shen Jiahao,Yang Yangyiwei,Gao Zhen,Liao Meng,Wu Jingxia,Wang Jiawei,Xu Xiaojie,Tong Qi,Zhang Bo,Wang Bingjie,Sun Xuemei,Zhang Lihua,Pei Qibing,Jin Dayong,Chen Peining,Peng Huisheng
Displays are basic building blocks of modern electronics 1 , 2 . Integrating displays into textiles offers exciting opportunities for smart electronic textiles—the ultimate goal of wearable technology, poised to change the way in which we interact with electronic devices 3 , 4 , 5 , 6 . Display textiles serve to bridge human–machine interactions 7 , 8 , 9 , offering, for instance, a real-time communication tool for individuals with voice or speech difficulties. Electronic textiles capable of communicating 10 , sensing 11 , 12 and supplying electricity 13 , 14 have been reported previously. However, textiles with functional, large-area displays have not yet been achieved, because it is challenging to obtain small illuminating units that are both durable and easy to assemble over a wide area. Here we report a 6-metre-long, 25-centimetre-wide display textile containing 5 × 10 5 electroluminescent units spaced approximately 800 micrometres apart. Weaving conductive weft and luminescent warp fibres forms micrometre-scale electroluminescent units at the weft–warp contact points. The brightness between electroluminescent units deviates by less than 8 per cent and remains stable even when the textile is bent, stretched or pressed. Our display textile is flexible and breathable and withstands repeated machine-washing, making it suitable for practical applications. We show that an integrated textile system consisting of display, keyboard and power supply can serve as a communication tool, demonstrating the system’s potential within the ‘internet of things’ in various areas, including healthcare. Our approach unifies the fabrication and function of electronic devices with textiles, and we expect that woven-fibre materials will shape the next generation of electronics.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2023
·
IF 5.8
Yu Changhao,Xu Yawen,Wei Yiping,Guo Yuxue,Wang Yi,Song Ping,Yu Jing
Microplastics (MPs) are known to cause liver toxicity as they can spread through the food chain. Most researches on their toxicity have focused on individual organs, neglecting the crucial “gut-liver axis”—a bidirectional communication pathway between the gut and liver. Probiotics have shown promise in modulating the effects of environmental pollutants. In this study, we exposed mice to Lactobacillus rhamnosus GG (LGG, 100 mg/kg b.w./d) and/or polystyrene microplastics (PS-MPs, 5 mg/kg b.w./d) for 28 d via gavage to investigate how probiotics influence live toxicity through the gut-liver axis. Our results demonstrated that PS-MPs induced liver inflammation (increased IL-6 and TNF-α ) and disrupted lipid metabolism. However, when combined with LGG, these effects were alleviated. LGG also improved colon health, rectifying ciliary defects and abnormal mucus secretion caused by PS-MPs. Furthermore, LGG improved gut microbiota dysbiosis induced by PS-MPs. Metabolomics and gene expression analysis ( Cyp7a1 and Cyp7b1 ) indicated that LGG modulated bile acid metabolism. In summary, LGG appears to protect the liver by maintaining gut homeostasis, enhancing gut barrier integrity, and reducing the liver inflammation. These findings confirm the potential of LGG to modulate liver toxicity caused by PS-MPs through the gut-liver axis, offering insights into probiotics' application for environmental pollutant detoxification.
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MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 3
·1-12
·
IF 5.833
Yao Chixuan,Liu Qingrun,Zhao Ning,Liu Jing-Min,Fang Guozhen,Wang Shuo
A simple dual-colour fluorescent nanoprobe has been designed composed of blue and yellow emission carbon quantum dots (CQDs). This system is inexpensive and easy to operate and was successfully employed for on-site measurements based on a smartphone app. The designed nanoprobe exhibited increased selectivity for Cr(VI), leading to a double stable response of the two CQDs. The dual-emission nanoprobe showed blue-violet fluorescence upon UV irradiation, and the fluorescent emission peaks were located at 418 nm and 552 nm. The blue light emission of CQDs was quenched with increasing Cr(VI) concentration due to the inner filter effect, whereas the yellow light emission was enhanced due to the aggregation-induced emission effect. The different responses of the dual emissions to Cr(VI) resulted in a fluorescent colour variation, thus enabling facile macroscopic visualization. With a smartphone, the change in the fluorescence colour could be observed more apparently than that of a single fluorescence nanoprobe, and the response increased linearly so that the nanoprobe could be applied to instantaneous measurements. Furthermore, the dual-emission nanoprobe was successfully employed for analysing food and water samples. Accurate concentrations were obtained by constructing a calibration plot using a fluorescence spectrometer and a smartphone app; the recoveries were 81.6% to 107.7%, and the relative standard deviation was below 3.6%. Therefore, this smartphone-integrated dual-emission detection system is promising as a new portable method for the on-site measurement of Cr(VI) ions. Graphical abstract
* Y-CQDs: yellow emission carbon quantum dots. B-CQDs: blue emission carbon quantum dots. B/Y-CQDs: a mixture of B-CQDs and Y-CQDs.
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JOURNAL OF POROUS MATERIALS
·
2021
·Vol. 28
·期 3
·895-903
·
IF 2.496
Xue Rui,Gou Hao,Liu Yinsheng,Rao Honghong
A layered triazinyl-COF(COF-TP) linked by − NH − bond was synthesized by condensation of 2,4,6 − trichloro − 1,3,5 − triazine and p − phenylenediamine. The BET specific surface area of COF-TP was 43.65 m 2 ·g − 1 and the pore size was about 13.658 Å. A N-doped porous carbon material(COF−TP − C) was prepared by heating the COF-TP at 700 °C under nitrogen protection. The BET specific surface area of COF−TP − C was 398.15 m 2 ·g − 1 and the pore size was about 10 Å. COF−TP exhibited a significant pseudocapacitance characteristic in 6 M K(OH) 2 electrolyte with the highest specific capacitance of 283 F·g − 1 at 1 A·g − 1 current density in three electrode configuration. The specific capacitance retention was about 88 % after 20,000 cycles. COF−TP − C showed the maximum specific capacitance of 449.5 F·g − 1 at the current density 1 A·g − 1 and a good cyclic performance of 77 % after 3000 GCD cycles(93 % after 1000 cycles) at the current density of 5 A·g − 1 , and retained 99.2 % after 5000 cycles at the current density of 1 A·g − 1 . Both COF−TP and COF−TP − C showed good capacitance performance and potential application in supercapacitors.
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危险品化学品经营许可证（带存储）