INDUSTRIAL CROPS AND PRODUCTS
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2018
·
IF 3.849
Shudong He,Zuoyong Zhang,Hanju Sun,Yuchen Zhu,Jinlong Zhao,Mingming Tang,Xiangyu Wu,Yanping Cao
Rapeseed peptide was used to produce flavor enhancer via heating with d -xylose at various temperatures (80–140 °C) at reaction pH 7.4 for 2 h with or without l -cysteine (RXC or RX). Effects of temperatures and l -cysteine on the Maillard reaction products (MRPs) were investigated by the determinations of pH, color, free amino acids (FAA), and volatile compounds, as well as E-nose and E-tongue analyses. Partial least square regression (PLSR) method was further applied to explore the correlations. Results suggested that pH decreased more significantly in RXCs, and a more dark brown color was observed in RXs with the increase of heating temperatures. The lowest bitterness MRPs were obtained by heating at 140 °C with cysteine (RXC-140), while the highest umami and saltiness sample was found in RX-100. Bitter and umami FAAs were present in greater amounts at higher temperatures with cysteine. Furthermore, RXC showed a meat-like flavor due to the generation of sulfur-containing compounds, and more furans and nitrogen-containing compounds were detected in RX, along with a caramel-like flavor.
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JOURNAL OF CHEMICAL AND ENGINEERING DATA
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2019
·
IF 2.298
Wenjun Zhu,Yuwen Fan,Qiang Xu,Xinxin Liu,Bin Heng,Wenge Yang,Yonghong Hu
In this research, solubility of l-tryptophan in water, methanol, ethanol, acetone, isopropanol, n-butanol, acetonitrile, ethyl acetate, organic pure solvent, and in three binary mixed solvents (water + acetonitrile), (water + methanol), and (water + isopropanol) at the temperature range of T = 278.15–333.15 K was determined with the gravimetric method. The experimental results show that the solubility of l-tryptophan in the above solvents increased as temperature rose, and it achieved the highest dissolution efficiency in water. The improved Apelblat model, the Buchowski–Ksiazaczak λh model, the Redlich–Kister (CNIBS/R–K) model, and the Jouyban–Acree model were used to nonlinearly fit the experimental data of solubility, and the consistency was good. The physicochemical information of l-tryptophan provided in this report may be helpful for its extraction/separation, recrystallization, purification, and formulation development.
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Polymer Chemistry
·
2018
·Vol. 9
·期 29
·3974-3986
·
IF 4.927
Pan Li,Yingying Song,Chang-Ming Dong
Controlled polymerization to synthesize hyperbranched polypeptides (HBPPs) with tunable degree of branching (DB) and molecular weight (MW) is still challenging in synthetic polypeptides and their biomaterials fields. A new photocaged amino acid monomer Nε-(1-(2-nitrophenyl)ethoxycarbonyl)-L-lysine-N-carboxyanhydride (NPE-Lys NCA) was designed. Without the addition of any catalyst/initiator and/or scavenger, lysine-based HBPPs were directly synthesized from the UV phototriggered ring-opening polymerization (ROP) of NPE-Lys NCA by a one-pot method and at room temperature. Their branched structures were fully characterized by means of 1H NMR, 1H,1H-COSY, 1H,13C-HSQC, and quantitative 13C NMR techniques. The effects of irradiation intensity and N2 flow rate on the phototriggered ROP and MWs, DBs, and conformations of the resulting polypeptides were investigated. At a lower irradiation intensity of 0.3 mW cm−2, the actual MWs (Mw,LLS) of P1 to P3 increased from 34.9 kDa to 62.8 kDa when N2 flow rate shifted to 300 mL min−1; DB reduced from 0.43 to 0.21; and the α-helix content rose from 35% to 63%, while the random coil decreased from 50% to 26%. By tuning the UV irradiation intensity and/or the N2 flow rate, this methodology can efficiently produce lysine-based HBPPs with tunable DBs of 0.21–0.54 and higher actual MWs of 13.7–62.8 kDa.
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Food & Function
·
2018
·Vol. 9
·期 4
·2270-2281
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IF 3.289
Han Wu,Xin Rui,Wei Li,Yu Xiao,Jianzhong Zhou,Mingsheng Dong
This study explored a novel strategy to develop solid-state whole-grain oats as a novel carrier of lactic acid bacteria and a nutraceutical supplement rich in ACE inhibitory peptides. Oats were fermented by Lactobacillus plantarum B1-6, Rhizopus oryzae, or a combination of L. plantarum B1-6 and R. oryzae. L. plantarum showed a much better growth performance in oats when it was combined with R. oryzae than when it was cultured alone, as evidenced by an increase in viable cell count to 9.70 log cfu g−1 after 72 h of fermentation. The coinoculated fermented oats (CFO) and the R. oryzae-fermented oats (RFO) were then selected for investigations on protein hydrolysis and on the functional properties of the released bioactive peptides. The results showed that the soluble protein contents changed from 7.05 mg g−1 to 14.43 and 10.21 mg g−1 for CFO and RFO, respectively. However, the degree of hydrolysis and the content of peptides with molecular masses less than 10 000 Da indicated that the CFO proteins can be degraded to a greater degree. As analyzed by electrophoresis and reversed-phase high-performance liquid chromatography, the protein and peptide profiles of CFO and RFO demonstrated that the proteins from CFO were more obviously hydrolyzed and more small peptides were obtained. In addition, both CFO and RFO presented higher ACE inhibitory activities than unfermented oats, whereas the protein extracts from CFO exerted a lower IC50 value of 0.42 mg protein per mL compared with the protein extracts from the other samples. This research has broadened our knowledge on the development of whole-grain oat products as a probiotic carrier and on the difference between mixed solid-state fermentation (SSF) and fungi SSF in terms of protein degradation and the capacity to release ACE inhibitory peptides. Our approach could be used to obtain probiotic food products and probably to develop oats as a potential therapeutic ingredient targeting hypertension.
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Journal of Materials Chemistry B
·
2018
·Vol. 6
·期 20
·3315-3330
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IF 4.776
Linlong Li,Xincui Shi,Zongliang Wang,Yu Wang,Zixue Jiao,Peibiao Zhang
Large-scale cell culture for cell expansion in tissue engineering is currently a major focus of research. One method to achieve better cell amplification is to utilize microcarriers. In this study, different amounts of poly(γ-benzyl-L-glutamate) (PBLG) (from 11 wt% to 50 wt%) were grafted on mesoporous hydroxyapatite (MHA) by the in situ ring opening polymerization of γ-benzyl-L-glutamate N-carboxyanhydride (BLG-NCA), and biodegradable and biocompatible PBLG-g-MHA microcarriers were directly fabricated using the oil-in-water (O/W) solvent-evaporation technique for bone tissue engineering. The amount of grafted PBLG could be controlled by adjusting the feed ratio of MHA and BLG-NCA. The relationships between sphere morphology and graft amount or solution concentration were explored. Furthermore, cytological assays were performed to evaluate the biological properties of the PBLG-g-MHA microcarriers. For a solution concentration of 3% (w/v) and PBLG graft amounts of 33 wt% and 50 wt%, the microspheres could be harvested with optimal spherical shapes. In vitro cell culture revealed that the PBLG-g-MHA microspheres had favorable properties for cell proliferation and significantly enhanced the osteogenic differentiation of MC3T3-E1 cells and bone matrix formation.
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International Journal of Nanomedicine
·
2016
·
IF 4.32
Fei Tong,Xiangyuan Tang,Xin Li,Wenquan Xia,Daojun Liu
The aim of this study was to observe the therapeutic effect of insulin-loaded linear poly(ethylene glycol)-brush-like poly(l-lysine) block copolymer poly(ethylene glycol)-b-(poly(ethylenediamine l-glutamate)-g-poly(l-lysine)) (PEG-b-(PELG-g-PLL) on renal ischemia/reperfusion-induced lung injury through downregulating hypoxia-inducible factor (HIF) as compared to free insulin. Sprague Dawley rats were pretreated with 30 U/kg insulin or insulin/PEG-b-(PELG-g-PLL) complex, and then subjected to 45 minutes of ischemia and 24 hours of reperfusion. The blood and lungs were collected, the level of serum creatinine and blood urea nitrogen were measured, and the dry/wet lung ratios, the activity of superoxide dismutase and myeloperoxidase, the content of methane dicarboxylic aldehyde and tumor necrosis factor-α, and the expression of HIF-1α and vascular endothelial growth factor (VEGF) were measured in pulmonary tissues. Both insulin and insulin/PEG-b-(PELG-g-PLL) preconditioning improved the recovery of renal function, reduced pulmonary oxidative stress injury, restrained inflammatory damage, and downregulated the expression of HIF-1α and VEGF as compared to ischemia/reperfusion group, while insulin/PEG-b-(PELG-g-PLL) significantly improved this effect.
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TALANTA
·
2017
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IF 4.162
Yue Hu,Anchen Fu,Zhaoyi Miao,Xiaojing Zhang,Tianlin Wang,An Kang,Jinjun Shan,Dong Zhu,Wei Li
Although ligand fishing has been shown to be an efficient technique for the identification of bioactive components from complex mixtures such as natural products , it cannot be applied to biomedical image processing. Herein, a specific fluorescent ligand fishing combined with in situ imaging approach is presented for the identification of heat shock protein 90 (Hsp 90) inhibitors from complex matrixes, Curcuma longa L. , using N-terminus immobilized Hsp 90α functionalized InP/ZnS quantum dots embedded mesoporous nanoparticles ( i.e. Hsp 90α (NT)-FQDNs) as extraction sorbents and fluorescent tracer. The fished ligands were identified by liquid chromatography time-of-flight/mass spectrometry (LC-TOF/MS) and gas chromatography-mass spectrometry (GC-MS). Moreover, in situ imaging by confocal laser scanning microscopy (CLSM) was applied for evaluating the effect of fished-ligands on bioactivity-induced apoptosis morphologically in HeLa cells. MTT assay verified the bioactivity of the ligands and molecular docking results further provided convincing information to verify the feasible binding mode between ligands and protein. Twelve ligands as potential Hsp 90 inhibitors were ultimately fished and identified from Curcuma longa L. crude extracts. The proposed approach based on Hsp 90α functionalized nanocomposites is superior in the combination of highly specific screening efficiency and concurrent visual in situ imaging, which could have great promise for the development of other plant-derived Hsp 90 inhibitors, and providing a rapid and reliable platform for discovering biologically active molecules in natural products.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Chenhan Xiong,Wei Cao,Jiaqi Chen,Yanqiu Yu,Xinming Lian,Rui Xue,Nan Chen,Zhaoyang Fan,Guoping Du
Designing rational heterostructures of high-performance electroactive materials on conductive substrates with hierarchical structures is critical for advancing electrochemical energy storage technologies. In this study, a unique spatial structure is fabricated by vertically aligning two-dimensional (2D) structures of Co-ZIF-L on conductive nickel foam (NF) substrate through interruption of ZIF-67 formation. This is followed by an innovative electrochemical synthesis method that disrupts unstable surface coordination bonds in Co-ZIF-L, enabling the in-situ generation of Co(OH) 2 . The resulting Co(OH) 2 @ZIF-L/NF binder-free electrodes feature a hierarchical spatial structure and are synthesized in approximately 30 s. These electrodes showcase exceptional area capacity of 3.1 C cm −2 at 1 mA cm −2 , attributed to their high specific surface area and layered architecture that promotes electrolyte penetration. Density Functional Theory (DFT) calculations reveal that the Co(OH) 2 @ZIF-L nanostructures have superior electrical conductivity compared to the individual components. Furthermore, a hybrid supercapacitor (HSC) based on Co(OH) 2 @ZIF-L/NF//AC exhibits an impressive energy density of 42 Wh kg −1 at a power density of 184.7 W kg −1 . This research provides new insights into the efficient synthesis of high-performance electroactive materials with unique spatial structures and expands the potential applications of ZIF materials.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2024
·Vol. 25
·期 12
·6754
·
IF 4.9
Danyang Zhang,Xiaoyu Wang,Kezhuo Sun,Jianli Guo,Jia Zhao,Yuesheng Dong,Yongming Bao
Osteoporosis, a prevalent chronic health issue among the elderly, is a global bone metabolic disease. Flavonoids, natural active compounds widely present in vegetables, fruits, beans, and cereals, have been reported for their anti-osteoporotic properties. Onion is a commonly consumed vegetable rich in flavonoids with diverse pharmacological activities. In this study, the trabecular structure was enhanced and bone mineral density (BMD) exhibited a twofold increase following oral administration of onion flavonoid extract (OFE). The levels of estradiol (E2), calcium (Ca), and phosphorus (P) in serum were significantly increased in ovariectomized (OVX) rats, with effects equal to alendronate sodium (ALN). Alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP) levels in rat serum were reduced by 35.7% and 36.9%, respectively, compared to the OVX group. In addition, the effects of OFE on bone health were assessed using human osteoblast-like cells MG-63 and osteoclast precursor RAW 264.7 cells in vitro as well. Proliferation and mineralization of MG-63 cells were promoted by OFE treatment, along with increased ALP activity and mRNA expression of osteoprotegerin (OPG)/receptor activator of nuclear factor-kappaB ligand (RANKL). Additionally, RANKL-induced osteoclastogenesis and osteoclast activity were inhibited by OFE treatment through decreased TRAP activity and down-regulation of mRNA expression-related enzymes in RAW 264.7 cells. Overall findings suggest that OFE holds promise as a natural functional component for alleviating osteoporosis.
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Chemical and Biological Technologies in Agriculture
·
2024
·Vol. 11
·期 1
·1-16
·
IF 5.2
Wang Yingying,Zhang Siyi,Ma Yuandan,Du Xianmin,Zong Qi,Lin Die,Lai Meng,Huang Tianyu,Luo Qingyun,Yang Lin,Li Zhanyong,Zuo Zhaojiang
Plant secondary metabolites can protect organisms against oxidative stress caused by adverse environmental conditions. Cinnamomum camphora (L.) J. Presl contains plentiful terpenoids and is subdivided into 5 chemotypes. To develop natural antioxidants using the plant terpenoids, the terpenoid composition and antioxidant abilities of methanol, ethyl acetate, n-hexane and petroleum ether extracts from linalool and eucalyptol chemotypes were investigated, and the solvent effects on in vitro antioxidant activity of 8 main terpenoids were analyzed. Meanwhile, the in vivo effects of two strong antioxidant terpenoids were evaluated. For the two chemotypes, the 4 solvents exhibited the same extracting effects on the terpenoid types, but methanol extracts contained the highest content of terpenoids, which should contribute to their strongest scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radicals. In linalool and eucalyptol chemotype extracts, linalool, eucalyptol, ocimene, α-pinene, D-limonene, terpinene, β-pinene and longifolene were the 8 main terpenoids. Among the 4 solvents, the 8 terpenoids showed the strongest free radical-scavenging activity with methanol as the reaction medium, which might result from strong-polarity methanol easily activating C = C unsaturated bonds in these compounds. This might also contribute to the strongest scavenging activity of methanol extracts against free radicals. Among these main components, ocimene and longifolene separately showed the strongest activity in scavenging DPPH and ABTS free radical. In in vivo assay, the two compounds significantly lowered the reactive oxygen species (ROS) levels in Chlamydomonas reinhardtii , a single-celled model organism, under H 2 O 2 stress, and the lowering effects gradually enhanced with increasing the compound concentration, resulting in the corresponding promoting effect on the algal growth. At the same concentration, longifolene showed the strongest effect on protecting the algal cells against oxidative stress. Therefore, methanol was suitable for extracting terpenoids in natural antioxidant development, and ocimene and longifolene were two strong antioxidant terpenoids without cell toxicity, with the latter having stronger in vivo antioxidant activity. Graphical Abstract
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ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Shan Huang,Wenqian Zhang,Bo Li,Guixin Li,Wei Ni,Yi Fang,Fuxiang Wei,Qi Xiao
Background Hexavalent chromium ions (Cr(VI)), a notorious toxic heavy metal pollutant with proven carcinogenicity, endangers human health and the environment. Meanwhile, l -ascorbic acid (L-AA), a vital biological antioxidant, has abnormal levels closely tied to various diseases. Developing efficient synchronous detection methods for these two key analytes is of great value in clinical and environmental monitoring. Traditional fluorescent nanomaterials, particularly those emitting in the blue/green region, suffer from limited tissue penetration depth and autofluorescence interference, hindering their application in deep-tissue imaging and real-time monitoring. Therefore, the development of red-emitting nanomaterials with enhanced optical properties and multifunctionality is crucial for advancing biosensing and bioimaging technologies. Results Herein, we present a facile hydrothermal synthesis of manganese-doped carbon dots (Mn-CDs) that exhibit red fluorescence ( λ ex / λ em = 520 nm/675 nm) and exceptional sensitivity for the simultaneous detection of Cr(VI) ions and L-AA. Mn-CDs were synthesized using o -phenylenediamine (OPD) and 2,5-diaminobenzenesulfonic acid (DSA) as precursors, along with manganese chloride tetrahydrate as a metal dopant source, achieving a high fluorescence quantum yield of 11 %. The Mn-CDs function as a dual-responsive "off-on-off" fluorescent sensor, demonstrating low detection limits of 0.44 μM for Cr(VI) and 1.11 μM for L-AA. Utilizing smartphone-integrated RGB analysis, the detection limits were further improved to 0.021 μM for Cr(VI) and 0.06 μM for L-AA. The Mn-CDs exhibit excellent biocompatibility and low cytotoxicity, enabling successful application in HeLa cells imaging and real-time intracellular monitoring of Cr(VI) and L-AA. Significance This study introduces a novel Mn-CDs-based fluorescent-colorimetric dual-mode probe that overcomes the limitations of traditional blue/green-emitting CDs by offering enhanced tissue penetration, minimized background interference, and multifunctional integration for biosensing and bioimaging. The integration of Mn-CDs with a smartphone-based analytical platform enhances their portability and user-friendliness for on-site detection, providing a valuable tool for heavy metal pollution monitoring and clinical biomarker detection. This work not only broadens the application scope of metal-doped carbon dots in biosensing but also provides a new strategy for environmental monitoring and clinical diagnostics, demonstrating significant application prospects in advancing biomedical research and clinical practice.
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Scientific Reports
·
2025
·Vol. 15
·期 1
·1-12
·
IF 3.9
Wan Meixia,Li Qing,Xiao Yudie,Zhou Dan,Lei Qianya,Wang Shu
Mori Fructus, rich in polysaccharides and polyphenols, has long been used in East Asia as a functional food and medicinal agent. In traditional Chinese medicine, it is used to treat various ailments like wasting-thirst syndrome and constipation. Studies suggest its extract fractions may alleviate metabolic syndrome symptoms by affecting gut microbiota. To explore this, fecal microbiota transplantation (FMT) was used in an experiment. Pseudo-germ-free mice were created with antibiotics and given a high-fat, high-sugar diet (HFD) to induce metabolic syndrome. Then, fecal bacterial infusions were transplanted. Results showed significant improvement in metabolic syndrome parameters in the FMT-MFPS(fecal microbiota transplantation-Mori Fructus polyphenols plus polysaccharides) group. Dyslipidemia, liver, and kidney injuries were modulated in treated mice. The PPARα/PGC-1α signaling pathway was activated. These findings indicate Mori Fructus extract fractions prevent metabolic syndrome via gut microbiota modulation, with effects sustained through FMT, providing a reference for prevention.
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