FOOD CHEMISTRY
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2025
·
IF 9.8
Yutong Ye,Jinhao Zhang,Panpan Chen,Yongli Ji,Chunyan Ma,Jun Guo,Yi Liu,Hongli Chen
We investigated the mechanism by which the number of phenolic hydroxyl groups in flavonoids regulates their interactions with β-lactoglobulin in food systems. Kaempferol, quercetin, and myricetin were selected as model flavonoids with increasing number of hydroxyl groups. Spectroscopy revealed that increasing the hydroxyl content enhanced protein fluorescence quenching, promoted β-sheet formation, and induced a more compact protein conformation (39.7–42.6 %). Thermodynamic results demonstrated that hydrogen bonding dominated the binding process and that the binding affinity increased with hydroxyl number (binding constant: 9.55 to 36.31 × 10 6 L·mol −1 ). Kinetic analysis showed that a higher hydroxyl content reduced the activation energy (from 40.9 to 25.4 kJ·mol −1 ), allowing flavonoid–protein binding to be completed within 5 s. Flavonoid-induced protein compaction improved the emulsion stability and antioxidant performance, depending on hydroxyl number. These findings reveal a structure–interaction–function relationship and provide guidance for designing flavonoid–protein delivery systems in food applications.
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Food Chemistry-X
·
2025
·
IF 8.2
Zhi-Wei Liu,Jun Lv,Chang Liu,Jun-Hu Cheng,Najla AlMasoud,Rana Muhammad Aadil,Xiu-Bin Liu
The characteristics of cold plasma (CP)-induced β-lactoglobulin-epigallocatechin gallate (β-LG-EGCG) covalent conjugates were investigated in comparison with the conventional free -radical (FR) and alkaline treatment (AT) methods. Results indicated CP achieved a high EGCG grafting degree (116.73 ± 0.82 μmol/g, equivalent to 38.91 ± 0.27 % of grafting) after 45 s treatment, comparable to AT (131.79 ± 1.75 μmol/g, equivalent to 43.93 ± 0.58 % of grafting) and far exceeding FR (18.29 ± 0.67 μmol/g, equivalent to 6.10 ± 0.22 % of grafting). In addition, CP conjugates exhibited 27.51 % less browning than AT counterparts. Different from AT and FR, CP uniquely expanded EGCG-binding sites to include hydroxyl- and cyclic alkyl-containing residues. Multiple structural analyses revealed covalent grafting with EGCG induced conformational unfolding of β-LG and exposed buried hydrophobic groups. The CP-induced β-LG-EGCG conjugates yielded improvements comparable to AT in emulsifying and antioxidant properties. Moreover, CP-induced conjugates exhibited superior digestibility and lower antigenicity during gastrointestinal digestion. These findings demonstrated CP is a robust means of promoting covalent grafting between proteins and polyphenols, with high efficiency and enhanced functionality.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Yao Wang,Xing Hu,Xuanang Liu,Zhichao Zhang,Guowen Zhang,Deming Gong,Peng Zhang
This study developed a β-LG-RA-PC emulsion ink as a proof-of-concept system for 3D/4D food printing. It characterized pH, salt, temperature, and oxidation stability; yam powder optimized texture and printability. Optimal pH 3 yielded minimal droplet size (24.99 μm), high viscoelasticity (G' > G”), and strong deformation resistance (σ 0 = 6.614 Pa), due to the shift in stabilization mechanism from electrostatic repulsion to dominant steric hindrance and three-dimensional network formation. Na + enhanced deformation resistance (σ 0 up to 11.320 Pa) but reduced viscosity. Heating decreased viscosity while maintaining σ 0 and improving recovery. Yam powder at 15 %–20 % ( w / v ), enabled precise printing of complex 3D structures, increasing yield stress (σ 0 = 16.138 Pa) and consistency ( k = 133.25 Pa·s n ) with pseudoplastic behavior ( n = 0.26–0.42). A curcumin-NaHCO 3 system achieved controllable color changes, establishing a proof-of-concept strategy for personalized nutrition and functional 4D-printed foods.
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Translational Research
·
2025
·
IF 5.9
Junjuan Lu,Caihong Liu,Ting Yuan,Honghui Yang,Li Zhang
Background Chronic obstructive pulmonary disease (COPD) is a chronic respiratory condition primarily caused by inhalation of harmful particles such as tobacco smoke. Cellular senescence serves as a key driver in its pathogenesis. Although endothelial progenitor cells (EPCs) have been shown to alleviate COPD by reducing inflammatory cell infiltration, the role and mechanisms underlying EPC senescence in this disease remain unclear. Methods A cigarette smoke (CS)-exposed COPD mouse model was established. Lung injury was assessed histologically, with concurrent quantification of neutrophil infiltration and cellular senescence levels in lung tissues. Pearson analysis evaluated the correlation between senescence severity and neutrophil numbers. In vivo neutrophil depletion was achieved using anti-Ly6G antibody, while GW4869 was used to inhibit exosome secretion from COPD-derived neutrophils. Neutrophils were then co-cultured with EPCs to assess their impact on EPC senescence and DNA damage. Proteomic analyses were employed to identify mechanisms of neutrophil-derived exosomes in COPD. Results COPD mice exhibited significant lung tissue damage, accelerated cellular senescence, and increased neutrophil infiltration. Senescence severity positively correlated with neutrophil proportion. Mechanistically, thrombospondin-1 (TSP-1) was highly expressed in COPD-derived neutrophils. Knockdown of neutrophil-derived exosomal TSP-1 alleviated EPC senescence. Furthermore, TSP-1 expression was regulated by transcription factor FOS, whereas TGF-β inhibition attenuated the promoting effects of TSP-1 overexpression on cellular senescence and lung injury in COPD mice. Conclusion This study demonstrates that neutrophil-derived exosomal TSP-1 aggravates EPC senescence and lung injury in COPD, revealing the pathogenic role of TSP-1 in disease progression and highlighting its potential as a therapeutic target.
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JOURNAL OF BIOSCIENCE AND BIOENGINEERING
·
2017
·
IF 2.24
Fei Chang,Saisai Xue,Xiaqing Xie,Wei Fang,Zemin Fang,Yazhong Xiao
Complicated purification steps, together with the fact that β-glucosidase has to be tolerant to ethanol restricts the application of β-glucosidase in isoflavone aglycone hydrolyzing process. β-Glucosidase Bgl1A(A24S/F297Y) is a promising enzyme in hydrolyzing isoflavones. In this work, six different carbohydrate-binding modules (CBMs), which were from 3 families, were fused to the C-terminal of Bgl1A(A24S/F297Y), respectively, to simplify the enzyme preparation process. The fusion proteins were expressed in Escherichia coli and adsorbed onto cellulose . The Bgl-CBM 24 was found to have the highest immobilization efficiency at room temperature within 1 h adsorption. Notably, 1-g cellulose absorbs up to 254.9 ± 5.7 U of Bgl-CBM 24 . Interestingly, the immobilized Bgl-CBM 24 showed improved ethanol tolerance ability, with the IC 50 of 35% (v/v) ethanol. Bgl-CBM 24 effectively hydrolyze soybean isoflavone glycosides . The hydrolysis rate of daidzin and gemistin was 85.22 ± 3.24% and 82.14 ± 3.82% within 10 min, with the concentrations of daidzein and genistein increased by 6.36 ± 0.18 mM and 3.98 ± 0.22 mM, respectively. In the repetitive hydrolytic cycles, the concentrations of daidzein and genistein still increased by 3.07 ± 0.24 mM and 1.94 ± 0.34 mM in the fourth cycle with 20% (v/v) ethanol. These results suggest that the immobilized Bgl-CBM 24 has excellent potential in the preparation of isoflavone aglycones.
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FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Ying Wu,Bin Li,Lei Zhou,Wei Liu,Chengmei Liu,Sangeeta Prakash,Junzhen Zhong
In our previous study, fibrous and granular whey protein self-assembled gels with low gel critical concentration (6%, w/w) and high gel mechanical strength were prepared, which showed good encapsulation and slow-release properties for epigallocatechin gallate. In order to better understand the gel formation mechanism, this study investigated the correlation between the gel quality and the protein structure based on kinetic pathways for fibrous (FBSG) and worm-like (WBSG) β-lactoglobulin self-assembled gels. The unheated samples of both FBSG and WBSG exhibited a loose structure, with the lowest hardness (6.733 g and 7.231 g, respectively) and rheological properties (G′ of 243 Pa and 79.9 Pa, respectively). As the heating time increased, the structures of FBSG and WBSG became denser, the hardness was increased and the rheological properties was improved. Hydrogen bonds and hydrophobic interactions played key roles in the formation of both FBSG and WBSG. Heat treatment had a greater influence on the formation of disulfide bonds in WBSG. In addition, we modelled and predicted about the gel delivery and slow-release properties using stepwise regression. The results showed that the three models about WBSG could be used for subsequent prediction, with adjusted R 2 of 0.989, 0.989, and 0.981, respectively, which was a good fit; the average absolute errors (measured and predicted) of the models regarding the encapsulation efficiency, loading capacity and gastric release rate of WBSG were −0.12%, −0.0061 μg/mg and −0.553%, respectively, with good model predictions. These findings provide valuable insights for the production and application of functional and special medical foods.
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FOOD CONTROL
·
2024
·
IF 5.6
Zeyu Qin,Qinbo Jiang,Meiyu Chen,Jiawen Li,Yang Li,Hui Zhang
This work aimed to investigat the properties of an antioxidant nanofiber coating incorporating zanthoxylum bungeanum essential oil and its function in the preservation of mangoes. Results showed that with the increase of zanthoxylum bungeanum essential oil-β-cyclodextrin inclusion complex (ZBEO-β-CD-IC) content in the nanofiber coating, the porosity, water vapor permeability, and oxygen permeability of the coating decreased, but the antioxidant activities increased. During storage, the increase of ZBEO-β-CD-IC content in the coating decreased weight loss and the respiration rate, and delayed the softening of tissue and yellowing of peel color. On the twelfth day, as the ZBEO-β-CD-IC content in the coating increased, the contents of flavonoids , total phenols, and ascorbic acid in mangoes increased from 2.36 × 10 −2 to 2.87 × 10 −2 g kg −1 , 23.76 × 10 −2 to 28.06 × 10 −2 g kg −1 , and 24.20 × 10 −2 to 39.38 × 10 −2 g kg −1 , respectively. Hydrogen peroxide content, superoxide anion rate, and malondialdehyde content decreased from 4.62 to 2.98 mmol kg −1 , 0.53 to 0.36 μmol min −1 kg −1 , and 8.22 to 6.24 μmol kg −1 , respectively. The activities of peroxidase , superoxide dismutase , catalase , ascorbate peroxidase were also inhibited after mangoes were coated in nanofibers containing ZBEO-β-CD-IC.
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Journal of the Iranian Chemical Society
·
2017
·Vol. 14
·期 9
·2017-2022
·
IF 1.407
Qin Xingxiu,Bakheet Almojtaba AbdAlkhalig Ahmed,Zhu Xiashi
Fe 3 O 4 @ionic liquids β-cyclodextrin polymer(Fe 3 O 4 @mono-6-deoxy-6-(1-ethyl-imidazolium)-β-cyclodextrin iodide polymer, Fe 3 O 4 @ILs-β-CDCP) was prepared. Magnetic solid phase extraction coupled with high-performance liquid chromatography for the separation/analysis of congo red (CR) in water and drysaltery was established. Fe 3 O 4 @ILs-β-CDCP showed a higher adsorption capacity toward CR. CR was adsorbed rapidly by Fe 3 O 4 @ILs-β-CDCP (adsorption efficiency: 95%) and eluted by ethanol (elution efficiency: 96%) at room temperature. Under the optimal conditions, preconcentration factor of the proposed method was 20-fold. The linear range, correlation coefficient ( R
2 ), detection limit (DL) and relative standard deviation were found to be 0.005–100.00 µg mL −1 , 0.9910, 1.8 g L −1 and 0.61% ( n = 3, c = 5.00 µg mL −1 ), respectively. The adsorption mechanism of CR on Fe 3 O 4 @ILs-β-CDCP was studied through the FTIR analysis. The accuracy of the developed method was confirmed by spiking city water, lake water, pond water and drysaltery. Fe 3 O 4 @ILs-β-CDCP can be used repeatedly for 10 times. This proposed method had been successfully applied to the determination of CR in real samples.
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Bioactive Carbohydrates and Dietary Fibre
·
2025
·
IF 3.6
Lan Zhao,Xinran Yang,Peijie Ye,Zhihai Lan,Jiale Cheng,Li Li,Jia Wu,Wei Xu,Huibin Chen
Qingke β-glucan (QBG), a gastrointestinal indigestible grain polysaccharide, is used as prebiotic and delivery carrier to mediate gut microbiota, while the mechanism of QBG of different MWs degraded by the gut microbiota remain uncertain. In this study, we investigated the impacts of QBG with different MWs on gut microbiota, SCFAs, and the degrading degree of polysaccharides in vitro fermentation. QBG groups significantly increased the total SCFAs, which is consistent with the results predicted by PICRUSt2 based on the KEGG pathway. The 27 functional genes associated with SCFAs metabolism were identified. Acetate and propionate synthesis was primarily mediated by the pta, ackA , and ACSS genes, whereas butyrate production mediated by the ptb, buk , and atoA genes. The low-MW QBG produced more SCFAs than the high-MW QBG after fermentation, suggesting that polysaccharide MW influences its biological functionality. These findings support polysaccharide-based gut microbiota-targeting drug and food development.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Chen Chen,Ximo Wang,Xinyue Wang,Geoffrey I.N. Waterhouse,Ruiqiang Wang,Yufeng Sun,Zhixiang Xu
Surface-enhanced Raman spectroscopy (SERS) is a powerful tool for the detection of target analytes in foods, with the realization of “hot spots” between plasmonic metal nanoparticles (NPs) being critical for ultrasensitive detection. Herein, we proposed a new aptamer conformation-mediated SERS “hot spots” strategy. Au NPs were functionalized with strands of complementary DNA containing β-phenethylamine aptamers and the Raman reporter 4-mercaptobenzonitrile (4-MBN). This allowed DNA-driven self-assembly of an Au dumbbell dimer-based SERS aptasensor for the detection of the β-phenethylamine. After selective recognition of β-phenethylamine by the aptamer, the aptamer folded, bringing AuNPs in the dimers close together to create SERS “hot spots”. This produced an intense SERS signal in the “biologically silent” region for 4-MBN (2225 cm −1 ), the intensity of which increased linearly with the logarithm of the β-phenethylamine concentration. Under the optimized conditions, the linear range of SERS aptasensor was 10 −6 to 10 −2 mg L −1 , with a low limit of detection of 3.8 × 10 −7 mg L −1 . Further, the developed SERS aptasensor was successfully employed to determine the β-phenethylamine in a range of food samples, with the results being identical to those obtained using a high-performance liquid chromatography method.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Jiaqi Wang,Binbin Li,Guilan Jiang,Silin Li,Jingyi Zhu,Jia Huang,Yuting Song,Zhuhong Ding
Lactobacillus acidophilus GIM1.208 (LA) β-glucosidase (BGL) converts glycosides from Rosa roxburghii Tratt (RRT) to release more active aglycones. This study purified LA BGL and investigated it interaction with rosamultin in RRT. The isolated and purified BGL in the study had higher purity and activity, and the proportion of ordered structure in its secondary structure was greater than 60%, indicating better structural stability. The hydrolysis of purified BGL hydrolyzed rosamultin to tormentic acid at a rate of 25.2% (p<0.01), and the hypoglycemic activity of tormentic acid was higher than that of rosamultin. Rosamultin causes BGL to undergo massive aggregation and enhances the hydrogen bonding network structure. Thermodynamic analyses indicate that their binding is a non-spontaneous exothermic entropy-decreasing reaction, with the main forces being hydrogen bonding, van der Waals forces, or protonation forces. Additionally, their thermodynamic binding constant Kd=2.976×10 -5 mol/L and dissociation constant K D =2.67×10 -5 mol/L indicate that the two have high affinity and strong interaction. In conclusion, BGL has the ability to hydrolyze rosamultin to release tormentic acid and they have a strong interaction. The results of this study can provide a reference for the study of glycoside changes and their interactions in RRT fermented by LA.
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LANGMUIR
·
2025
·
IF 3.9
Linting Jiang,Lu Yang,Xue Yi,Shengtao Zhang,Hongru Li,Zhiyong Wang,Fang Gao
Zinc corrosion, hydrogen evolution reaction, uneven deposition, and dendrite growth on the zinc anodes are the key factors restraining the electrochemical performance and cycling stability of the aqueous zinc-ion batteries. In this study, learned from the synial membrane, a tiny amount of natural amino acid β-alanine (β-Ala, 0.089 wt %) was introduced as the additive in ZnSO4 electrolyte for strengthening the kinetics of the zinc anode as well as enhancing the performance of zinc ions batteries. A number of modern surface techniques and surface electrochemical analyses were employed to reveal the fundamental reasons for the strengthened zinc anode by β-Ala in ZnSO4 electrolyte. The results show that β-Ala could be adsorbed on zinc electrode surface through intermolecular chelation, which might regulate the chemical environments of the electrolyte and promote uniform deposition of zinc ions. Hence, the β-Ala adsorption film on zinc electrode could suppress the hydrogen evolution reaction and the formation of zinc dendrites, thereby significantly improving the deposition/stripping process of the zinc anode. In particular, the strong hydrogen bonding could restrain the migration of H2O molecules approaching the zinc anode surface, preventing the invasion of water to the zinc electrode surface. Therefore, the addition of dilute β-Ala in the ZnSO4 electrolyte might remarkably prolong the life span of Zn||Zn symmetric batteries to 5000 h under 1 mA cm–2 and 1 mAh cm–2, and to 450 h under 5 mA cm–2 and 3 mAh cm–2 at 298 K, which is much longer than the zinc–zinc symmetric cells including the bare ZnSO4 electrolyte (only 95 h at 5 mA cm–2 and 3 mAh cm–2, and 200 h at 1 mA cm–2 and 1 mAh cm–2). Furthermore, β-Ala was found to significantly improve the cycling stability of Zn||Cu asymmetric cells and Zn||V2O5 full cells. This study provides an effective method for engineering electrolytes to inspire rechargeable zinc-ion batteries by selecting ideal natural biomolecules as the electrolyte additives.
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