INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Zhouya Bai,Yunhui Zhang,Honglin Lan,Xingguo Li,Jiahao Wan,Yanbin Wang,Jinying Guo,Denglin Luo
Chinese yam ( Dioscorea opposita Thunb .) is a traditional medicinal and edible homologous material, with polysaccharides being its primary bioactive components. In this study, two polysaccharides (CYP and CYPP) were isolated and purified from Chinese yam ( cv. Tiegun ) and its peel, and their anti-diabetic activities were investigated. Structural characterization revealed that the weight-average molecular weights of CYP and CYPP were 74,746 Da and 118,083 Da, respectively. Monosaccharide composition analysis showed that both polysaccharides had similar compositions, primarily consisting of mannose, galactose, and galacturonic acid. Further nuclear magnetic resonance (NMR) spectroscopy analysis demonstrated that CYP mainly contained β-type glycosidic bonds, while CYPP possessed both α- and β-type glycosidic bonds. In a type 2 diabetes mellitus (T2DM) mouse model induced by high-fat diet (HFD) combined with streptozotocin (STZ), both polysaccharides exhibited significant hypoglycemic and hypolipidemic activities. Further investigations revealed that CYP and CYPP could effectively improve metabolic profiles in diabetic mice, significantly regulating two key metabolites, UDP- N -acetylglucosamine and uridine diphosphate glucose (UDP-glucose) while influencing pentose and glucuronate interconversions and starch and sucrose metabolism pathways. Additionally, 16S rRNA sequencing results demonstrated that CYP and CYPP significantly ameliorated gut microbiota dysbiosis in T2DM mice. Notably, compared with CYP, CYPP exhibited superior hypoglycemic and hypolipidemic efficacy, and this functional discrepancy may be closely associated with its higher molecular weight characteristics and distinctive viscoelastic properties. In conclusion, this study demonstrates that CYPP is a promising prebiotic agent for managing T2DM by modulating metabolic pathways and gut microbiota, offering new insights for the valorization of agricultural by-products.
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DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2022
·Vol. 46
·期 5
·2335-2350
·
IF 3.925
Wen-Yan Wang,Zi-Hui Yang,A-Liang Li,Qing-Song Liu,Yue Sun,Wen Gu
A series of novel 2-amino-4-aryl-pyrimidine derivatives of ursolic acid were designed, synthesized, and evaluated for their anticancer activities against four cancer cell lines (MCF-7, HeLa, HepG2, and A549) and a human hepatocyte cell line (LO2) via MTT assay. Among these derivatives, compound 7b exhibited potent cytotoxic activity against MCF-7 and HeLa cells with IC50 values of 0.48 ± 0.11 and 0.74 ± 0.13 μM, respectively, and substantially lower cytotoxicity to LO2 cells. Further cellular mechanism studies in MCF-7 cells elucidated that compound 7b could inhibit cell migration, induce cell cycle arrest at S phase and trigger mitochondrial-related apoptosis by increasing the generation of intracellular ROS and decreasing the mitochondrial membrane potential (MMP), which was associated with upregulation of the protein expression level of Bax and downregulation the level of Bcl-2 and the activation of caspase cascade. Western blot analyses also revealed that compound 7b could simultaneously suppress RAS/Raf/MEK/ERK and PI3K/AKT/mTOR signaling pathways, which could be responsible for the induction of apoptosis. Molecular docking study revealed that MEK1 kinase could be one of the possible targets of the title compounds. These results offered a promising scaffold for the investigation of novel targeted anticancer agents.
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高影响因子
Polymers
·
2022
·Vol. 14
·期 24
·5466
·
IF 4.967
Changcai Teng,Xiongyan Meng,Yeqin Hu,Hongzhao Mao,Huiting Li,Jing Yang,Tiantian Sun,Shuai Meng,Chengli Zong
Small synthetic TLR7/8-agonists can be used as vaccine adjuvants to enhance cell and humoral-mediated immune responses to specific antigens. Despite their potency, after local injection they can be dispersed to undesired body parts causing high reactogenicity, limiting their clinical applications. Here we describe a vaccination strategy that employs the covalent conjugate of a mannose and TLR7/8 agonist as a vaccine adjuvant to take advantage of mannose binding C-type lectins on dendritic cells to enhance the vaccine’s immunogenicity. The mannose-TLR7/8 agonist conjugate can self-assemble into nanoparticles with the hydrophilic mannose on the outside and hydrophobic TLR7/8 agonist inside. Although its ability to stimulate HEK-BlueTMhTLR7/8 cells dropped, it can efficiently stimulate mouse bone marrow-derived dendritic cells as indicated by the up-regulation of CD80 and CD86, and higher cytokine expression levels of TNF-α, IL6, and IL-12p70 than the native TLR7/8 agonist. In vivo, vaccination using the SARS-CoV-2 RBD trimer as the antigen and the conjugate as the adjuvant induced a significantly higher amount of IgG2a. These results suggest that the mannose-TLR7/8-agonist conjugate can be used as an effective vaccine adjuvant.Keywords:adjuvants;mannose;TLR7/8 agonist;immune responses;cytokines;antibody
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PubMed
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 10
·1-10
·
IF 5.833
Chen Rongyuan,Kan Lun,Duan Fenghe,He Linghao,Wang Minghua,Cui Jing,Zhang Zhihong,Zhang Zhonghou
A novel label-free surface plasmon resonance (SPR) aptasensor has been constructed for the detection of N-gene of SARS-CoV-2 by using thiol-modified niobium carbide MXene quantum dots (Nb 2 C-SH QDs) as the bioplatform for anchoring N-gene-targeted aptamer. In the presence of SARS-CoV-2 N-gene, the immobilized aptamer strands changed their conformation to specifically bind with N-gene. It thus increased the contact area or enlarged the distance between aptamer and the SPR chip, resulting in a change of the SPR signal irradiated by the laser (He-Ne) with the wavelength ( λ ) of 633 nm. Nb 2 C QDs were derived from Nb 2 C MXene nanosheets via a solvothermal method, followed by functionalization with octadecanethiol through a self-assembling method. Subsequently, the gold chip for SPR measurements was modified with Nb 2 C-SH QDs via covalent binding of the Au-S bond also by self-assembling interaction. Nb 2 C-SH QDs not only resulted in high bioaffinity toward aptamer but also enhanced the SPR response. Thus, the Nb 2 C-SH QD-based SPR aptasensor had low limit of detection (LOD) of 4.9 pg mL −1 toward N-gene within the concentration range 0.05 to 100 ng mL −1 . The sensor also showed excellent selectivity in the presence of various respiratory viruses and proteins in human serum and high stability. Moreover, the Nb 2 C-SH QD-based SPR aptasensor displayed a vast practical application for the qualitative analysis of N-gene from different samples, including seawater, seafood, and human serum. Thus, this work can provide a deep insight into the construction of the aptasensor for detecting SARS-CoV-2 in complex environments. Graphical abstract
A novel label-free surface plasmon resonance aptasensor has been constructed to detect sensitively and selectively the N-gene of SARS-CoV-2 by using thiol-modified niobium carbide MXene quantum dots as the scaffold to anchor the N-gene-targeted aptamer.
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PubMed
高影响因子
JOURNAL OF THE AMERICAN CERAMIC SOCIETY
·
2024
·Vol. 107
·期 12
·8281-8290
·
IF 3.5
Shumin Duan,Zhenjun Qing,An Liu,Haiyan Li,Yan Xue
Ultra-low temperature sintered ceramics, Li 2 (Mg 1− x Ca x ) 2 (MoO 4 ) 3 ( x = 0.025–0.100), were synthesized using the solid-phase method. XRD analysis revealed that adding Ca 2+ increased the second-phase CaMoO 4 content. Particularly, Li 2 (Mg 0.975 Ca 0.025 ) 2 (MoO 4 ) 3 ceramics exhibited excellent properties: ε r = 9.31, Q × f = 55,400 GHz, and τ f = −38.9 ppm/°C. Incorporating Ca 2+ significantly improved the performance of ceramics. The microwave dielectric properties of Li 2 (Mg 0.9 Ca 0.1 ) 2 (MoO 4 ) 3 ceramics sintered at 650°C showed enhancements: ε r of 9.55, Q × f of 63,500 GHz, and τ f of −10.8 ppm/°C. The rise in ε r can be attributed to polarizability, while a higher packing fraction led to a larger Q × f value, and the reduced distortion of the [Li/Mg/CaO 6 ] octahedra enhanced the τ f value. Moreover, the increased of CaMoO 4 also positively influenced the ceramic's properties. Furthermore, in accordance with the principles of complex chemical bonding, the elevation of ionicity, lattice energy, and bond energy of the Mo–O bond contributed to the enhancement of ε r , Q × f , and τ f , respectively. Raman spectroscopy showed a positive correlation between dielectric loss and full width at half maximum of the Raman peak. Good chemical compatibility between silver and Li 2 (Mg 0.9 Ca 0.1 ) 2 (MoO 4 ) 3 ceramic was demonstrated through our co-firing experiment.
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高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2019
·Vol. 166
·期 2
·B42
·
IF 3.12
Yongling Du,Yifeng He,Zhixiang Zheng,Xuan Shen,Yaxin Zhou,Tingxia Wang,Zhengping Zhu,Chunming Wang
Co(OH)2 nano particles (NPs)/three-dimensional graphene frameworks (3DGFs) nano-materials was fabricated via electrochemical methods. The Co(OH)2 NPs/3DGFs hybrid retain the 3DGFs's structure and have superior catalytic activity for glucose oxidation. The electrochemical sensors based on Co(OH)2 NPs/3DGFs exhibited high sensitivity of 2410 μA cm−2mM−1, wide linear range of 2 μM to 1.4 mM, low detection limits of 0.67 μM and a quick response (less than 3s) to glucose. The Co(OH)2 NPs/3DGFs were used as a high performance platform to detect glucose in human serum, fetal bovine serum, and human urine. The excellent catalytic activity of Co(OH)2 NPs/3DGFs is attributed to the high surface area of 3DGFs and the highly catalytic activity of Co(OH)2 NPs as well as the synergistic effects between Co(OH)2 NPs and 3DGFs. Importantly, the electrochemical glucose sensor could be renewable only being refreshed in 0.1 M NaOH for ten minutes.
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高影响因子
IEEE Photonics Journal
·
2017
·Vol. 9
·期 1
·1-10
·
IF 2.291
Panchun Gu,Huajiang Wei,Guoyong Wu,Zhouyi Guo,Hongqin Yang,Yonghong He,Shusen Xie
The objective of this paper is to evaluate the penetration and accumulation of SiO
2
nanoparticles (NPs) in vitro human placental tissue as a function of NP size and electroporation (EP) and microneedle (MN) treatments. The results show that the penetration and accumulation of SiO
2
NPs has a significant influence on the optical properties. Optical coherence tomography (OCT) monitoring and diffuse reflectance (DR) spectra measurements indicate that 30 nm SiO
2
NPs have a tendency to generate greater signal intensities, while 15 nm NPs penetrated faster and had a higher penetration depth. The reduction in average attenuation coefficients of human placental tissue when the SiO
2
NPs size is constant is found to show the following trend: SiO
2
NPs in combination with EP and MN treatment > SiO
2
NPs in combination with MN > SiO
2
NPs with EP > only SiO
2
NPs. It can be concluded that EP and MN treatments dramatically increase the penetration of SiO
2
NPs into human placental tissue. The results prove that OCT and DR spectra can be combined with EP and MN treatments to establish a theoretical basis for the safe use of SiO
2
NPs on human placental tissue and can potentially become a powerful tool for early diagnosis and monitoring of tissue diseases.
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高影响因子
Arabian Journal of Chemistry
·
2021
·
IF 5.165
Na Li,Kai Yuan,Tengjia Gao,Shun Li,Jianliang Qin,Yirong Zhu,Jingjing Du,Lijian Xu,Jianxiong Xu
Design of electrode materials with excellent electrochemical properties for advanced energy storage device are important to the sustainable development of energy resources. In this manuscript, a novel hierarchical nanoporous carbon@nickel hydroxide (HNCMs@Ni(OH) 2 ) composites were prepared through simple in-situ chemical deposition of Ni(OH) 2 nanocrystals on HNCMs. By tailoring the deposition amount, core–shell structured rambutan-like HNCMs@Ni(OH) 2 microspheres with hairy nickel hydroxide nanocrystal covered on HNCMs were obtained. Ascribed to the high specific capacitance of nickel hydroxide and the interconnected hierarchical porous structure of HNCMs, the resultant rambutan-like HNCMs@Ni(OH) 2 microspheres as electrode materials display outstanding electrochemical properties including high specific capacity of 248.9 mAh/g at 1 A/g and good capacitance retention of 62.9% at 20 A/g. Furthermore, a hybrid supercapacitor with rambutan-like HNCMs@Ni(OH) 2 microspheres as cathode and HNCMs as anode were assembled and showed a comparable energy density of 41.3 Wh/kg at the power density of 173.3 W/kg and high capacity retention of 85.2% after 20,000 cycles at 5 A/g. As a proof of concept example, the fabricated hybrid supercapacitor can be utilized as energy source to instantaneous light up a red LED indicator. We envisioned that the as-prepared rambutan-like HNCMs@Ni(OH) 2 microspheres have great potential in constructing of advanced energy storage devices.
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高影响因子
JOURNAL OF PORPHYRINS AND PHTHALOCYANINES
·
2021
·
IF 1.811
Safi Ullah,Keisuke Ogumi,Xuelin Zhen,Huan Wang,Yutaka Matsuo
A Suzuki–Miyaura coupling reaction was performed to synthesize 5,15- meso -bis(2-chlorothiophen-3-yl)porphyrins in order to obtain a building block for further functional porphyrins. Photophysical and electrochemical properties were investigated to understand the effect of 2-chloro substituted thiophenes which are connected at their 3-position to the meso -position of the porphyrin. A computational calculation with counterpoise method was demonstrated to estimate the relative intermolecular interaction in order to compare solubility. Installed chloro atoms at the 2-position of thiophen-3-yl decreased in intermolecular interaction which caused an increase in solubility.
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高影响因子
CyTA-Journal of Food
·
2020
·
IF 1.653
Yongzhen Wang,Kai Zhu,Deguo Wang
The objective of this study was to develop a reliable visual method for rapid detection of donkey components to protect consumers from commercial adulteration. Six specific loop-mediated isothermal amplification (LAMP) primers targeted at donkey mitochondrial cytochrome b gene were designed, the mitochondrial DNA extraction simplified, the LAMP reaction system optimized, the specificity verified with mitochondrial DNA of horse, pork, cow, sheep, chicken, duck, and rabbit as negative controls, the detection limit determined with gradient dilution of adulterated meat with donkey meat, and the visual LAMP method for detection of donkey-derived ingredient in common meat products established. The results showed that the modified mitochondrial DNA extraction method was simple and repeatable, and the visual LAMP method with 4-(2-pyridylazo)-resorcinol sodium salt as indicator can accurately and specifically detect the donkey meat in common meat products, 1% detection limit. The study provided a promising solution for facilitating the surveillance of the commercial adulteration in processed meat.
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高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2022
·
IF 6.633
Xintong Wang,Jiang Yang,Xu Chen,Wei Ding
The inhibition properties of 2-mercaptobenzimidazole (MBI) and its synergism with oleic imidazoline (OIM) for carbon steel in CO 2 -saturated brine solution were investigated by electrochemical, weight loss measurements, surface analysis and quantum chemical calculations. The effectiveness of MBI for OIM was also compared to the other sulfur-containing corrosion inhibitors such as 2-mercaptothanol (ME), mercaptoacetic acid (TGA), and thiourea (TU). The results show that a mixture of MBI and OIM gave a better synergistic corrosion inhibition than that of ME, TGA, and TU. OIM is protonated and coupled with MBI by hydrogen bonding to co-adsorb on the carbon steel surface. MBI adsorbs on Fe by a bidentate binding N S bridge connection, which effectively prevents the carbon steel from CO 2 corrosion.
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高影响因子
JOURNAL OF THE AMERICAN CERAMIC SOCIETY
·
2019
·Vol. 103
·期 2
·1046-1056
·
IF 3.094
Xiaohui Feng,Le Wang,Ran Wei,Ziyan Wang
Non-contact temperature sensors based on the fluorescence intensity ratio (FIR) have been widely investigated owing to their high sensitivity and reliable real-time monitoring. Herein, the SiO 2 -coated LiY(MoO 4 ) 2 @SiO 2 :Er 3+ ,Yb 3+ phosphor was investigated as an optical thermometry material, which was synthesized using the conventional solid state reaction and coated by a facile wet chemical route. The effect of surface modification on FIR was systematically characterized by structural analyses and spectral measurements and the temperature-dependent up-conversion FIR was investigated from 303 to 603 K under a 980 nm laser excitation. The results showed that the FIR value was thermally stable and the SiO 2 coating led to a higher FIR sensitivity as well as a higher saturation threshold. This work would pave a way to design interesting optical thermometry materials in up-conversion phosphors with better properties.
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高影响因子
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