SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Huaze Shao,Hong Yan,Qing Tang,Shiquan Zheng,Hanren Chen,Lihong Liu
The overexpression of human epidermal growth factor receptor 2 (HER2) has been linked to aggressive breast cancer and worse clinical outcomes. However, accurately measuring HER2 remains challenging, due to its low serum content. Herein, we developed a novel method for HER2 detection in serum by the combination of aptamer with C-Ag + -C structure, Ag + -released hybridization chain reaction (Ag + -HCR) and coprecipitation of Ag + and gold nanoparticles (AuNPs) in the solution including Eosin Y (EY) and AgI colloids. Following the aptamer bound to HER2, signal amplification occurred through the release of numerous Ag + by Ag + -HCR. This process subsequently induced AuNPs aggregation on the surface of AgI colloids, resulting in reduced fluorescence intensity of EY in the supernatant. The reduced value of EY fluorescence intensity in the supernatant was proportional to the level of HER2. Under optimized reaction conditions, a wide detection range was obtained between 0.09 and 9000 pg/mL with a limit of detection of 11.7 fg/mL in diluted human serum, and 96 serum samples in a 96-well plate could be measured simultaneously by a microtiter plate reader within 2 h. Moreover, the quantitative results of HER2 were consistent with those obtained using the enzyme-linked immunosorbent assay kit ( r = 0.9995). Therefore, leveraging the delicate design of Ag + -HCR signal amplification, this enzyme-free, label-free, and effective method has demonstrated to be capable of detecting HER2 in serum due to its high sensitivity. Remarkably, this method may be extended to detect other cancer-related proteins and genes by replacing the corresponding target sequences.
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Lina Dai,He Wang,Yanduo Liu,Liru Cui
Direct light-driven methane selective conversion into useful chemicals remains a major challenge due to the need for photocatalysts with high photoactivity and selectivity in activating inert C-H bonds. In this study, we have developed highly photoactivity and selective photocatalytic CH 4 oxidation into C 2 H 6 using Ag-Mg tandem sites co-modified ultrathin ZnO hybrid photocatalysts in a flow reactor. Our system achieves an C 2 H 6 production rate of over 1860 μmol/g/h with 93 % selectivity, which is significantly higher than the efficiency of state-of-the-art photocatalytic systems. Various in-situ characterizations and theoretical studies reveal that the Ag-Mg tandem sites co-modified leads to enhanced photogenerated charge separation efficiency, while maintaining high ethane production rate owing to the strong selective adsorption capacity for methane and oxygen of the doped Mg sites and oxygen vacancy. Furthermore, the Ag nanoparticles cocatalyst can facilitate the removal of activated CH 4 in the form of methyl radicals in the gas phase, thereby promoting C 2 H 6 formation and inhibiting CH 4 overoxidation to produce CO 2 . Overall, these findings provide novel insights into the photocatalytic conversion of CH 4 on Ag/Mg-ZnO. These results offer valuable possibilities for developing more effective catalysts for the conversion of CH 4 to higher value-added products.
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Bin Shang,Yanbing Wang,Peng Yang,Bo Peng,Ziwei Deng
Surface-enhanced Raman scattering (SERS) sensor allows for fingerprinting and label-free molecular detection with a high sensitivity, but the simple fabrication of SERS active substrates with a well-control over the SERS performance, e.g. an ultra-high sensitivity, superior reproducibility and long-term stability remain a great challenge. Here, we introduce an easy strategy for synthesizing polydopamine-Ag microbowl/nanoparticle array (PDA-Ag MNA) substrates with a tunable superhydrophobic surface, applicable for ultra-highly sensitive SERS detection. First, the periodically dented PDA films are prepared by polymerizing dopamine onto 2D polystyrene colloidal crystals formed at the liquid/air interface, and followed with an in-situ reduction of Ag ions to Ag nanoparticles after removing polystyrene. The surface morphology, i.e. microbowl/nanoparticle structure, is well-controlled, therefore, allowing that the SERS activity of the substrate is under control. Inspired by the superhydrophobic hierarchical surface of the rose petals, this analogous MNA surface is subsequently endowed to be superhydrophobic. This way, the SERS detection of ultra-dilute solute (10 −14 M) can be achieved by concentrating analytes within a localized region. Moreover, this substrate also shows a superior reproducibility in result and durability in use. This strategy exhibits a realistic paradigm for further improving the sensitivity of the SERS technique.
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Rongyang Liu,Li Jiang,Zizhen Yu,Xufeng Jing,Xiu Liang,Dan Wang,Bo Yang,Chengxing Lu,Wei Zhou,Shangzhong Jin
Recent years, two-dimensional transition metal carbonitrides, MXene, have emerged as SERS substrates due to their unique optical and electrical properties. In order to combine the advantages of metal nanoparticles and MXene for SERS substrates, this paper prepared an MXene (Ti 3 C 2 T x ) -Ag nanoparticles (NPs) hybrid biosensor through in-situ electrostatic self-assembly method, in which the citrate-coated Ag NPs are loaded on the negatively surface of Ti 3 C 2 T x flakes through positively charged PDDA polymer. The characterizations show that a large number of Ag NPs uniformly adsorb on both side of Ti 3 C 2 T x nanosheets. The prepared platform exhibits excellent SERS performance, long-term stability and good uniformity by detecting 4-MBA molecules. The electromagnetic field distribution was simulated to theoretically show the Raman enhancement mechanism of this platform. In order to explore the possible application of the sensor in clinical diagnosis and monitoring of biological environment, the platform was used for biomolecule detection. The detection limit of adenine molecules can be as low as 10 −8 M. For dopamine molecules with concentration ranging from 5 × 10 -6 M to 5 × 10 −8 M, the SERS results show a linear relationship between peak intensity and concentration of dopamine molecules. The biosensor was applied for serum detection. The results suggest that MXene-PDDA-Ag NPs hybrid platform could be used as a sensitive and uniform biosensor for label-free quantitative detection of bio-molecular based on SERS method, which could be applied in the field of biotechnology.
✓ 已验证
DOI
高影响因子
LANGMUIR
·
2022
·
IF 4.331
Shenjie Li,Xiaoyu Gong,Zhiqiang Li,Minghui Yu,Yanyan Chen,Hao Yu,Shuang Wang,Hongyu Shao,Minghao Dou,Yuye Cheng
A novel interfacial reaction nucleation mechanism for the preparation of water-soluble Ag–In–S quantum dots (AIS QDs) was proposed in which interfacial acid regulates the concentration of hydroxide ions outside the complex and sulfur sources attack cations at the interface of the complex, covalent bonds between cations and sulfur sources are formed at the interface of the complex, and the nucleation and growth of crystals is finished at room temperature. By bypassing the heating process normally necessary for crystal nucleation and growth, AIS QDs can be produced on a large scale under simple, mild conditions. At the same time, the characteristics of this mechanism enable AIS QDs to be directly synthesized in an organic pollutant solution. This study represents a significant advance in the mechanism of crystal synthesis and contributes to the photocatalytic decomposition of organic pollutants from theory to practice.
✓ 已验证
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Huarong Liao,Miaomiao Li,Yuxiang Wang,Guan Wang,Liujun Yang,Junxia Shao,Cheng Zhang,Hua Li,Jianmei Lu
Refining the visible light absorption and optimizing the separation of photogenerated electron-hole pairs are pivotal in enhancing the photocatalytic activity of composites. A novel Z-scheme plasma photocatalyst, Ag/Ag 3 PO 4 /In-MOF (A/AP/In), was developed in this study. Experimental results demonstrated that A/AP/In exhibited the highest photocatalytic activity for BPA among all samples tested, achieving complete degradation of 50 ppm BPA within 30 min, as well as its apparent degradation rate was about 11 times higher than that of pure Ag 3 PO 4 and 4 times higher than that of pure In-MOF. The enhanced photocatalytic activity can be mainly attributed to the existence of Z-type heterojunctions and the surface plasmon resonance (SPR) effect induced by Ag nanoparticles , which facilitate efficient migration and separation of photogenerated carriers. Meanwhile, the incorporation of Ag nanoparticles enhances visible light absorption and protects Ag 3 PO 4 from photocorrosion in the composites. Moreover, A/AP/In maintains the large specific surface area of In-MOF and provides abundant reactive active sites. In addition, the photocatalytic performance of A/AP/In was above 90 % after five consecutive cycles confirming its good stability. Overall, this study proposes a novel approach for constructing bifunctional composites with efficient adsorption/catalytic degradation.
✓ 已验证
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2011
·
IF 3.068
Jingpeng Yuan,Yongchao Lai,Junling Duan,Quanqin Zhao,Jinhua Zhan
A mono-6-thio-β-cyclodextrin-modified silver film was synthesized via galvanic displacement on copper foil. The prepared silver films could enrich non-polar polychlorinated biphenyls (PCBs) molecules from hydrophilic phase using thiolate β-cyclodextrins (SH-β-CDs) as receptors. The components of as-prepared Ag-coated-Cu (Ag–Cu) film were confirmed by powder X-ray diffraction (XRD). Both surface-enhanced Raman spectroscopy (SERS) and energy dispersive X-ray spectroscopy (EDS) measurements gave strong evidences that the thiolated β-cyclodextrins (SH-β-CDs) had been immobilized on the surface of silver film. Compared to the substrates prepared in the absence of SH-β-CD, the surface morphology of the CD-modified Ag films was obviously changed. The interfacial enrichment and the capability of substrates to form inclusion complexes with PCBs molecules were tested by using PCB-15 (4,4′-dichlorobiphenyl) as the probe molecules via SERS technique. The measured SERS spectra could distinguish the PCB-15 molecules at micro-molar level according to the most intense CCC bending in-plane mode of PCBs. The enhancement factor (EF) of the SERS substrates for PCB-15 was 1.2 × 10 5 , which was comparable with a number of previous reports.
✓ 已验证
DOI
高影响因子
ENVIRONMENTAL POLLUTION
·
2019
·
IF 5.714
Yi Shen,Chao Zhu,Baoliang Chen
To improve catalytic performance and stability of Ag nanoparticles (Ag NPs), a facile ultrasonication-assisted chemical reduction method was developed to fabricate reduced graphene oxide (rGO) aerogels loaded with 1–3 nm Ag NPs under the normal temperature and pressure. The ultrasonication facilitated the dispersion of Ag(I) in the form of silver ammonia and anchored onto GO nanosheets . Ag(I) and GO were simultaneously reduced to Ag(0) immobilizing onto 3D rGO hydrogels within the heterogeneous liquid phase, and ultimately formed 3D rGO-Ag NPs aerogels . The 3D rGO-Ag NPs aerogels displayed superb catalytic performance for the reduction of nitrobenzene (NB), 1,3-dinitrobenzene (DNB) and 4-nitrophenol (NP) into aniline, 1,3-diaminobenzene and 4-aminophenol, respectively. The individual reduction rate K obs for NB, DNB and NP were 0.168 h −1 , 0.109 h −1 and 0.092 h −1 , which were much higher than those of other Ag NPs-based materials. Moreover, the immobilization of 1–3 nm Ag NPs in 3D rGO-Ag NPs was stable during the whole reduction reaction without aggregation and leaching. The high stability of Ag NPs in 3D rGO-Ag NPs and superb performance on catalytic reduction of nitroaromatic compounds (NACs) could be concluded into ultrasonication influence in the preparation procedure and synergistic effect of Ag NPs and 3D rGO in the catalytic reduction process. The simple ultrasonication-assisted chemical reduction approach provided a scaled-up application prospect in catalytic reduction of NACs by metal nanoparticle catalysts.
✓ 已验证
DOI
高影响因子
JOURNAL OF RAMAN SPECTROSCOPY
·
2021
·Vol. 53
·期 2
·211-221
·
IF 3.133
Nan Jia,Yinran Xiong,Ying Wang,Siqian Lu,Ruoqiu Zhang,Yan Kang,Yiping Du
A label-free and novel surface-enhanced Raman scattering (SERS) method has been studied to rapidly detect common fish pathogenic bacteria ( Escherichia coli , Vibrio anguillarum , Vibrio harveyi , Edwardsiella piscicida , and Pseudomonas plecoglossicida ). The marriage of magnetic nanospheres (Fe 3 O 4 ) with polyethylenimine (PEI) could trigger surface charge modifications of Fe 3 O 4 , making it effective to capture negatively charged bacteria. After the rapid bacterial capture procedure within 10 min, Au@Ag core-shell bimetallic nanoparticles served as hotspot providers to facilitate the enhancement of biological Raman signals. The results demonstrated that the minimum detection limit of E. piscicida could be as low as 10 5 cfu/ml. The peak intensity at 1628 cm −1 ( E. piscicida ) showed a linear relationship with the logarithm of the bacterial concentration ( R 2 = 0.997). A mimic test was conducted by spiking E. piscicida into tap water. Linear discriminant analysis (LDA) was also harnessed to classify different types of fish bacteria. And the positive rates of the training set and the prediction set were 100% and 80%, respectively. The whole experimental procedure only takes 20 min. This method opens a new avenue for the rapid detection and identification of fish pathogenic bacteria.
✓ 已验证
DOI
高影响因子
ACS Omega
·
2022
·
IF 4.132
Xianglong Li,Yongna Zhang,Weicai Kong,Jing Zhou,Teng Hou,Xianggui Zhang,Lele Zhou,Mingbo Sun,Shu Liu,Bin Yang
In this research, centrifugally spun ultrafine composite starch/polyvinyl alcohol (ST/PVA) fibers with high water stability were prepared by cross-linking with a mixture of glutaraldehyde and formic acid in the form of vapor phase. The effect of cross-linking temperature combined with time on the water stability, crystal structure, and thermal properties of fibers was investigated to obtain the optimum parameters. On this basis, we further prepared Ag-loaded ST/PVA fibers with different contents of nano silver. The structure and properties of Ag-loaded fibers, which cross-linked under the optimum parameters, were analyzed. As a result, the Ag-loaded fibers exhibited excellent water stability and mechanical properties and possessed inhibition zone diameters of 3 and 2 mm to Escherichia coli and Staphylococcus. aureus, respectively. The antibacterial property of the Ag-loaded ST/PVA fibers provided a new route for developing less costly antibacterial fiber materials in the future.
✓ 已验证
DOI
高影响因子
ACS Applied Bio Materials
·
2021
·
IF 0
Menghui Wan,Haodong Zhao,Lichao Peng,Yanbao Zhao,Lei Sun
The fabrication of highly active and free-standing surface-enhanced Raman scattering (SERS) substrates in a simple and low-cost manner has been a crucial and urgent challenge in recent years. Herein, SiO2 nanofiber substrates modified with size-tunable Ag nanoparticles were prepared by the combination of electrospinning and in situ chemical reduction. The results demonstrate the presence and uniform adsorption of Ag nanoparticles on the SiO2 matrix surface. The free-standing composite nanofibrous substrates show high-performance SERS response toward 4-mercaptophenol and 4-mercaptobenzoic acid, and the detection limit can be as low as 10–10 mol/L. Most importantly, the as-prepared substrate as a versatile SERS platform can realize label-free detection of bio-macromolecules of bacteria, i.e., Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). Furthermore, the substrates also possess outstanding antibacterial activities against S. aureus and E. coli. Briefly, the significance of this study is that size-tunable Ag nanoparticles can be decorated on SiO2 nanofiber surfaces with triethanolamine as a bridging and reducing agent through a one-pot reaction, and the as-prepared nanofibrous membranes are expected to act as a candidate for label-free SERS detection as well as antibacterial dressing.
✓ 已验证
DOI
BIOSENSORS & BIOELECTRONICS
·
2011
·
IF 5.361
Xiaochun Jiang,Kun Chen,Heyou Han
A novel electrochemical biosensor was developed for detecting short DNA oligonucleotide of Bacillus thuringiensis (Bt) transgenic sequence based on Ag nanoparticle aggregates. To fabricate this DNA biosensor, the thiol-modified capture DNA (cDNA) was first anchored on gold (Au) electrode, and then the target DNA (tDNA) was hybridized with the immobilized cDNA. Subsequently, the probe DNA (pDNA) functionalized by biotinylated Ag nanoparticle was associated with the fixed tDNA, and the single Ag nanoparticle label was obtained (cited as SAg label). Finally, dissociative biotinylated Ag nanoparticle was bound to the resultant biotinylated SAg label assembled on Au electrode by virtue of bridge molecule streptavidin (SA) through biotin–SA specific interaction, which could lead to in situ aggregate of Ag nanoparticles on Au electrode and induce a novel tag including multiple Ag nanoparticles (cited as MAg tag). The novel tag exhibited excellent electroactive property in the solid-state Ag/AgCl process and was successfully applied to Bt transgenic sequence assay. A detection limit of 10 fM was achieved, which was improved by three orders of magnitude as compared to the SAg label. Furthermore, this novel DNA biosensor demonstrated a good selectivity towards tDNA.
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）