SENSORS AND ACTUATORS B-CHEMICAL
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2021
·
IF 7.46
Yu Zhang,Xiao-Fan Zhang,Qian Chen,Xiao-Qun Cao,Shi-Li Shen
Nitroreductase (NTR) is a highly expressed endogenous enzyme in hypoxic tumor cells, which is associated with the hypoxic degree in tumors. Therefore, it is particularly important to develop low-toxicity and high-sensitivity fluorescent probes for NTR. Herein, we report an NTR-activated near-infrared probe with good selectivity and sensitivity. More importantly, the background fluorescence signal is effectively reduced due to the quenching effect of nitro group as well as the spironolactone (ring-closed) form of rhodamine moiety. Upon reaction with NTR, the probe exhibits a remarkable fluorescence off-on response at 740 nm (about 32-fold intensity enhancement), with an extremely low detection limit of 1.09 ng/mL. Moreover, the probe is able to target mitochondria. With this probe, we have achieved the visualization of NTR overexpression in hypoxic cancer cells, as well as in hypoxic tumors in vivo as revealed in the tumor-bearing mouse model. By using this probe, some essential information may be revealed about hypoxia-relevant diseases.
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高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2020
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IF 3.99
Haiyan Tian,Dong Xiang,Bo Wang,Weimin Zhang,Congfa Li
The purpose of this study is to prepare carriers to encapsulate tea polyphenols (TPPs) to increase their stability and retention rate. TPPs were embedded in binary gum mixtures. TPPs were slowly released from these mixtures, and the slowest release rate was obtained when the release was from the xanthan gum (XG)/locust bean gum (LBG) mixture at a ratio of 6:4. TPPs were further encapsulated in water-in-oil (W/O) emulsion containing the XG/LBG mixture, and the effects of emulsifier concentration and XG/LBG mixture (6:4) on the stability of W/O emulsion were examined. With increasing emulsifier concentration, the stability increased. It was also observed that the XG/LBG mixture could enhance the stability of the emulsion. Finally, encapsulation of TPPs in the W/O emulsion containing XG/LBG mixture resulted in a high TPPs retention rate of over 70 % after 30 days of storage at 25 °C. The W/O emulsion containing XG/LBG mixture in the dispersed phase has a potential use as an active carrier.
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Frontiers in Plant Science
·
2019
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IF 4.106
Zhao Mingyue,Jin Jieyang,Gao Ting,Zhang Na,Jing Tingting,Wang Jingming,Ban Qiuyan,Schwab Wilfried,Song Chuankui
Glycosyltransferases (UGTs) play diverse roles in cellular metabolism by altering regulatory metabolites activities. However, the physiological roles of most members of UGTs in crops in response to abiotic stresses are unknown. We have identified a novel glycosyltransferase CsUGT78A14 in tea crops, an important economic crops, whose expression is strongly induced by cold stress. Biochemical analyses confirmed that CsUGT78A14-1 showed the highest activity toward kaempferol and is involved in the biosynthesis of kaempferol-diglucoside, whereas the product of CsUGT78A14-2, which differs from CsUGT78A14-1 by a single amino acid, was identified as 3-O-glucoside. The accumulation of kaempferol monoglucosides and diglucosides was consistent with the expression levels of CsUGT78A14 in response to cold stress, as well as in different tissues and genotypes of tea plants. Down-regulation of CsUGT78A14 resulted in reduced accumulation of flavonols, reactive oxygen species (ROS) scavenging capacity and finally reduced tea plant stress tolerance under cold stress. The antioxidant capacity of flavonols aglycon was enhanced by glucosylation catalyzed by CsUGT78A14. The results demonstrate that CsUGT78A14 plays a critical role in cold stress by increasing flavonols accumulation and ROS scavenging capacity, providing novel insights into the biological role of UGTs and flavonoids in plants.
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高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2022
·Vol. 29
·期 13
·19890-19897
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IF 5.19
Zhao Anran,Miao Jingjing,Liu Liru,Pan Luqing
Retinoid X receptor (RXR) has been found to be a major target in various processes of endocrine disruption from the exposure to organotin compounds (OTCs), including imposex in gastropod mollusks. It was also reported in bivalves that OTCs caused intersex and skewed sex ratio. In order to evaluate the effect of these ligand-like OTCs, we constructed a reconstituted yeast system (CfRE system) based on GAL4 yeast two-hybrid principle using scallop Chlamys farreri retinoid X receptor ( Cf RXR a ) and retinoid X response element (RXRE) to investigate the ligand-induced transactivation of Cf RXR a . Responses of Cf RXR a to 9-cis retinoic acid (9cRA) and tested four OTCs showed concentration-dependent response which is comparable with reported RXRa in vitro assay of human and gastropods. The detective limits of the CfRE system were found to be 100 nM for 9cRA and 10–1000 nM for the tested OTCs. While the tested non-Sn endocrine disrupting chemicals, including Benzo[a]pyrene, 2,4-Dichlorophenol, Nonylphenol, and Tetrabromobisphenol A, showed no effect on Cf RXR a response. The present assay system may provide a valuable tool for screening assessments of unidentified environmental ligand chemicals on bivalve mollusks. It is also useful for comparison of sensitivity differences among species exposed to EDCs.
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高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2025
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IF 4.9
Haiyun Zhang,Xiujuan Wang,Yilun Wang,Yuanyuan Zhao,Yibing Chen,Yuanlu Cui,Qiangsong Wang
The combination of Tanshinone IIA (Tan IIA) and quercetin (QU) demonstrates therapeutic potential for acne treatment. However, their poor water solubility and limited epidermal permeation hinder their effective deposition and delivery within the skin. A novel composite system, referred to as TQ hydrogel, was developed to co-deliver Tan IIA and QU. This system integrated nanoemulsion and thermosensitive gels. The nanoemulsion formulation was optimized through pseudo-ternary phase diagram. The TQ hydrogel was prepared by incorporating a poloxamer-based matrix with the drug-loaded nanoemulsion. The morphology, in vitro drug release profiles, antioxidant capabilities, and antimicrobial efficiency of the TQ hydrogel were assessed. Furthermore, the in vivo anti-acne effects and underlying mechanisms of the TQ hydrogel were evaluated. The prepared nanoemulsion were spherical in shape with a mean particle size of 60 nm and zeta potential was about −8.12 mV. The TQ hydrogel exhibited temperature-triggered sol-gel transition at 30.4 °C. The cumulative release of QU and Tan IIA from TQ hydrogel was 88.69 % and 17.61 % for 24 h, respectively. TQ hydrogel displayed notable antioxidant activity and bactericidal properties. In vivo , TQ hydrogel significantly ameliorated the inflammatory microenvironment of acne tissues, suppressed the expression of androgen receptors (AR), and showed promising potential in reducing acne-induced scarring. TQ hydrogel, as a co-delivery carrier integrating nanoemulsion and thermosensitive gels, represented an ideal platform for acne treatment.
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Food Science & Nutrition
·
2025
·Vol. 13
·期 12
·e71310
·
IF 3.8
Fan Cao,Hua Wu,Zhen He,Mengxin Di,Bin Liu,Zhenwei Chen,Jieming Xie,Yonghong Zhang,Xinhua Ma
Melia azedarach L., also known as the chinaberry tree, has long been used in traditional medicine to treat inflammatory conditions. In this study, we preliminarily investigated the anti-inflammatory effect of 21 α -methylmelianol (MMN), a tirucallane-type triterpenoid isolated from M. azedarach , in lipopolysaccharide (LPS)-mediated RAW264.7 macrophages in vitro and in a dextran sodium sulfate (DSS)-stimulated ulcerative murine colitis model in vivo. MMN reduced LPS-stimulated nitric oxide production without inducing cytotoxicity. It significantly attenuated LPS-induced protein expression of reactive oxygen species and effectively suppressed the production of pro-inflammatory factors, including tumor necrosis factor-alpha (TNF- α ), interleukin (IL)-6, and IL-1 β . In addition, mechanistic investigations showed that MMN inhibited signal transduction and activator of STAT3 phosphorylation and activation of nuclear factor κ B (NF- κ B). In mice with DSS-induced colitis, MMN attenuated DSS-mediated pathological manifestations, including colon shortening, body weight loss, and histopathological alterations. Its in vivo effects were associated with the regulation of pro- and anti-inflammatory cytokine levels through suppression of the NF- κ B signaling cascade and downstream inflammatory pathways. Collectively, these findings provide compelling evidence of the anti-inflammatory properties of MMN and highlight its therapeutic potential for the management of inflammatory disorders.
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高影响因子
Frontiers in Pharmacology
·
2025
·
IF 4.8
Xiaoli Deng,Chunjie Hu,Lin Li,Guixian Yang,Chikun Li,Wanxun Zhang,Guangyu Cheng
Background Lung cancer is a leading cause of cancer-related deaths, with cisplatin being a cornerstone of treatment. However, resistance to cisplatin presents a significant challenge. β-elemene, a natural compound, has demonstrated potential to reverse cisplatin resistance. LncRNA LINC00511 has been implicated in cisplatin resistance through its role in activating the PI3K/AKT/mTOR pathway, which supports tumor survival and proliferation. Purpose This study aims to investigate the mechanism by which β-elemene overcomes cisplatin resistance in lung cancer by regulating LINC00511. Methods Human lung adenocarcinoma cells (A549 and A549/DDP) were treated with β-elemene and cisplatin. Cell proliferation and apoptosis were assessed using CCK-8, EdU staining, and flow cytometry. LINC00511 expression was measured by qRT-PCR, and protein levels of PI3K, AKT, and mTOR were evaluated via Western blot. A xenograft model was used to confirm in vivo effects. Results β-elemene significantly enhanced cisplatin-induced apoptosis in A549/DDP cells, reduced LINC00511 expression, and inhibited the PI3K/AKT/mTOR pathway. LINC00511 knockdown further potentiated these effects, both in vitro and in vivo . Xenograft models confirmed the enhanced anti-tumor effects of the combination treatment. Conclusion β-elemene overcomes cisplatin resistance in lung cancer by downregulating LINC00511 and inhibiting the PI3K/AKT/mTOR pathway. These findings propose a promising therapeutic strategy for treating cisplatin-resistant lung cancer.
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高影响因子
Journal of Cleaner Production
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2025
·
IF 10
Guangzhao Ran,Xiaohua Li,Shanshan Shao,Shiliang Wu
The convergence of agricultural waste recycling and retired lithium-ion battery (LIBs) upcycling presents a critical pathway for sustainable environmental management.This study systematically evaluates two innovative strategies, in-situ thermal reduction (ISTR) and acid-leaching loaded catalysts (ALLC), for repurposing cathode materials nickel manganese cobalt oxide, lithium cobalt oxide and lithium iron phosphate (NCM, LCO, LFP) in cascade biomass conversion systems for hydrogen production. Through controlled pyrolysis experiments, it was demonstrated that NCM cathodes undergo carbothermal reduction at 500 °C, with derived NiO species significantly enhancing biomass pyrolysis gas cracking with hydrogen yield of 19.45 mmol/g. Comparative acid leaching analysis shows that metal recovery behaviors are significantly different. Among them, the leaching efficiency of LCO/LFP using oxalic acid is higher. The leached LCO catalyst exhibits excellent carbon-carbon bond cleavage ability, achieving a hydrogen yield of 26.2 mmol/g in the reforming process. Crucially, ISTR-NMC cathode systems facilitates biomass bond cleavage and cracking, whereas ALLC LCO and LFP exhibit enhanced catalytic reforming performance, and an optimized integration work combining ISTR-treated NMC with ALLC-LCO, achieving a record 28.5 mmol/g hydrogen production through synergistic cascade reactions.This study reveals metal speciation-dependent catalytic mechanisms and closed-loop design principles for synergistic battery recycling and biomass valorization, aiming at circular economy solutions for dual waste streams via hybrid thermal-catalytic processing.
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高影响因子
JOURNAL OF NUTRITION
·
2025
·
IF 3.8
Lemei Li,Jinyang Wu,Huilei Hao,Ying Wang,Shaoying Hou
Background Gestational diabetes mellitus (GDM) is prone to induce hepatic injury in offspring. Sialic acid (SA) exhibits hepatoprotective effects, while its potential against GDM-induced liver injury in offspring remains unclear. Objective The present study aimed to investigate the hepatoprotective effects of maternal sialic acid (SA) supplementation in GDM-exposed offspring and to explore its underlying mechanisms. Methods C57BL/6J mice were divided into three groups: a control (NC-m) group, a gestational diabetes mellitus (GDM-m) group, and a gestational diabetes mellitus plus sialic acid (GDM+SA-m) group. The GDM-m group was induced using high-fat diet combined with intraperitoneal streptozotocin (STZ) injection. The GDM+SA-m group was administered N-glycolylneuraminic acid (Neu5Ac), human milk SA, via intragastric gavage at a dose of 50 mg/kg during pregnancy and lactation. Offsprings from three groups were fed a control diet until 8 weeks of age, with 20, 20, and 19 in the NC-o, GDM-o, and GDM+SA-o groups, respectively. Liver was obtained and analyzed for HE staining, AST and ALT, oxidative stress markers, inflammatory cytokines, and non-targeted metabolomics. Results and Conclusion s: Compared with the NC group, GDM significantly elevated liver transaminase levels and increased inflammatory and oxidative stress in offspring mice, with more pronounced effects observed in males. SA intervention partially attenuated this damage. Concurrently, metabolomic analysis revealed that SA significantly regulated lipid metabolism and taurine/hypotaurine pathways. The results suggested that maternal SA intervention ameliorated GDM-induced offspring liver injury potentially by regulating hepatic lipid and taurine/hypotaurine metabolism, providing a novel mechanistic insight for early-life intervention in GDM.
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高影响因子
BIOMEDICINE & PHARMACOTHERAPY
·
2024
·
IF 6.9
Junyu Wang,Chaojie Yang,Zhixin Liang,Junping Sun,Mingyue Zhang,Shaofu Qiu,Xinying Du,Xi He,Xiaoying Pang,Xidong Ma,Mei Xie,Xinjie Han,Ru Fan,Enlu Zhou,Hairong Yu,Danyang She,Hongbin Song,Jianxin Wang
Human adenovirus (HAdV) infection is a major cause of respiratory disease, yet no antiviral drugs have been approved for its treatment. Herein, we evaluated the antiviral and anti-inflammatory effects of cyclin-dependent protein kinase (CDK) inhibitor indirubin-3′-monoxime (IM) against HAdV infection in cells and a transgenic mouse model. After evaluating its cytotoxicity, cytopathic effect reduction, antiviral replication kinetics, and viral yield reduction assays were performed to assess the anti-HAdV activity of IM. Quantitative real-time polymerase chain reaction (qPCR), quantitative reverse transcription PCR (qRT-PCR), and western blotting were used to assess the effects of IM on HAdV DNA replication, transcription, and protein expression, respectively. IM significantly inhibited HAdV DNA replication as well as E1A and Hexon transcription, in addition to significantly suppressing the phosphorylation of the RNA polymerase II C-terminal domain (CTD). IM mitigated body weight loss, reduced viral burden, and lung injury, decreasing cytokine and chemokine secretion to a greater extent than cidofovir. Altogether, IM inhibits HAdV replication by downregulating CTD phosphorylation to suppress viral infection and corresponding innate immune reactions as a promising therapeutic agent.
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高影响因子
Frontiers in Microbiology
·
2021
·
IF 5.64
Luo Yeshen,Liu Fei,Song Jianhua,Luo Qian,Yang Yonggang,Mei Chengfang,Xu Meiying,Liao Bing
Functional microorganisms play a vital role in removing environmental pollutants because of their diverse metabolic capability. Herein, a function-oriented fluorescence resonance energy transfer (FRET)-based graphene quantum dots (GQDs-M) probe was developed for the specific identification and accurate sorting of azo-degrading functional bacteria in the original location of environmental samples for large-scale culturing. First, nitrogen-doped GQDs (GQDs-N) were synthesized using a bottom-up strategy. Then, a GQDs-M probe was synthesized based on bonding FRET-based GQDs-N to an azo dye, methyl red, and the quenched fluorescence was recovered upon cleavage of the azo bond. Bioimaging confirmed the specific recognition capability of GQDs-M upon incubation with the target bacteria or environmental samples. It is suggested that the estimation of environmental functional microbial populations based on bioimaging will be a new method for rapid preliminary assessment of environmental pollution levels. In combination with a visual single-cell sorter, the target bacteria in the environmental samples could be intuitively screened at the single-cell level in 17 bacterial strains, including the positive control Shewanella decolorationis S12, and were isolated from environmental samples. All of these showed an azo degradation function, indicating the high accuracy of the single-cell sorting strategy using the GQDs-M. Furthermore, among the bacteria isolated, two strains of Bacillus pacificus and Bacillus wiedmannii showed double and triple degradation efficiency for methyl red compared to the positive control (strain S12). This strategy will have good application prospects for finding new species or high-activity species of specific functional bacteria.
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JOURNAL OF DISPERSION SCIENCE AND TECHNOLOGY
·
2020
·
IF 1.701
YangLing Wang,Chen Wu,XiaoYa Zhou,MoRan Zhang,Yi Chen,ShaoPing Nie,MingYong Xie
Gallic acid or its alkyl ester was added in combination with α-tocopherol in the oil-in-water (O/W) emulsion to investigate their interaction in emulsions. Results showed that all the tested gallate esters (propyl, octyl and dodecyl gallate) showed synergistic antioxidant activities with α-tocopherol, and propyl gallate with the shortest alkyl chain length possessed the highest synergistic ability. The interaction efficiency was consistent with the order of their apparent static quenching constants obtained from fluorescence quenching studies. This suggested that the antioxidant synergy could be due to their molecular interactions. With further confirmation by antioxidant decomposition studies, the mechanism for such antioxidant synergy in O/W emulsions was proposed to begin with the initial quick oxidation of α-tocopherol into α-tocopherol quinine, resulting in its oriented exposure to the interfacial membrane of O/W emulsion and, subsequently, being reduced by propyl gallate esters in the interfacial layer to regenerate α-tocopherol.
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