JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2024
·
IF 2.8
Fei Yang,Yi-Yi Cao,Jing Xi,Yang Luan,Na Li,Xin Dong,Xin-Yu Zhang
1,3-Butadiene (BD) is a carcinogenic air pollutant. N -acetyl- S -(4-hydroxy-2-buten-1-yl)-L-cysteine (MHBMA3 or 4HBeMA), an urinary BD metabolite with unspecified configuration, is considered the most sensitive BD biomarker and has been used in routine biomonitoring since 2012. However, two issues remain unaddressed: why its concentrations are unusually high relative to other urinary BD biomarkers and why some authors reported no detection of the biomarker whereas other authors readily quantitated it. To address the issues, we synthesized and structurally characterized the authentic trans - and cis -isomers of MHBMA3 (designated NE and NZ, respectively), developed an isotope-dilution LC-MS/MS method for their quantification, and examined 67 urine samples from barbecue restaurant personnel (n = 47) and hotel administrative staff (n = 20). The restaurant personnel were exposed to barbecue fumes, which contain relatively high concentrations of BD. The results showed that NE and NZ had highly similar NMR spectra, and were difficult to be well separated chromatographically. The NMR data showed that the MHBMA3 isomer investigated in most previous studies was NE. We did not detect NE and NZ in any samples; however, an interfering peak with varying heights was observed in most samples. Notably, under the chromatographic conditions used in the literature, the peak exhibited indistinguishable retention time from that of NE. Thus, it is highly likely that the interfering peak has been mis-identified as NE in previous studies, providing a reasonable explanation for the high MHBMA3 concentration in urine. The contradiction in the presence of MHBMA3 in urine was also caused by the mis-identification, because the researchers who reported the absence of MHBMA3 were actually detecting NZ. Thus, we clarified the confusion on MHBMA3 in previous studies through correctly identifying the two MHBMA3 isomers. The presence of NE and NZ in human urine warrants further investigations.
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高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Zongwei Hao,Jiameng Wang,Zhaofeng Li,Zhichao Shen,Hui Xu,Zhongyun Zhao,Zongjun Wu,Huijun Sun,Yixuan Xu,Mingming Zheng,Yibin Zhou,Yiqun Du,Zhenyu Yu
With the increasing number of patients with type II diabetes associated with starchy foods, the inhibition of starch digestion has become one of the most important strategies for limiting postprandial glucose elevation. However, indirect inhibition of starch digestibility through the starch digestive enzymes pathway remains poorly studied. Based on this, we found that L-theanine (THE) had the potential to inhibit starch digestive enzyme activity and investigated the mechanism based on multi-spectral and computer simulations. The results showed that the half-inhibitory concentration (IC 50 ) values of THE on α-amylase and α-glucosidase were 1.22 mmol/L and 2.77 mmol/L, respectively, which exhibited a mixed type of inhibition. Fluorescence quenching results and analyses showed that the mechanism of inhibition of THE with digestive enzymes had both static quenching, mainly based on non-covalent interactions such as van der Waals forces and hydrogen bonding, with binding energies of −3.19 and −7.76 kcal/mol, respectively (Molecular docking results). The multi-spectral analysis showed that the binding of THE changed the secondary structure of starch digestive enzymes and the conformation of polypeptide chains, thus inhibiting the activity. The findings of this study are expected to guide the development of food products with hypoglycaemic function using THE.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Yanxia Yu,Chunying Zuo,Mingrui Li,Yuanyuan Tang,Lingxi Li,Fang Wang,Shuting Zhang,Baoshan Sun
In this study, an effective method for preparation of bioactive galloylated procyanidin B2-3′-O-gallate (B2-3′-G) was first developed by incomplete depolymerization of grape seed polymeric procyanidins (PPCs) using l-cysteine (Cys) in the presence of citric acid. The structure–activity relationship of B2-3′-G was further evaluated in vitro through establishing lipopolysaccharide (LPS)-induced inflammation in RAW264.7 cells. The results suggested that the better protective effects of B2-3′-G against inflammation were attributed to its polymerization degree and the introduction of the galloyl group, compared to its four corresponding structural units. In vivo experiments demonstrated that the B2-3′-G prototype was distributed in plasma, small intestine, liver, lung, and brain. Remarkably, B2-3′-G was able to penetrate the blood–brain barrier and appeared to play an important role in improving brain health. Furthermore, a total of 18 metabolites were identified in tissues. Potential metabolic pathways, including reduction, methylation, hydration, desaturation, glucuronide conjugation, and sulfation, were suggested.
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JOURNAL OF ETHNOPHARMACOLOGY
·
2021
·
IF 4.36
Chunlian Li,Yijie Luo,Weicheng Zhang,Qiuyang Cai,Xianyi Wu,Zekai Tan,Rui Chen,Zehao Chen,Shimin Wang,Lanyue Zhang
Ethnopharmacological relevance Essential oils (EOs) are important active ingredients in Chinese herbal medicine. Cymbopogon citratus (DC.) Stapf, Cinnamomum cassia (L.) Presl, Salvia japonica Thunb., and Rosa rugosa Thunb. are used in local and traditional medicine to treat inflammation-related diseases. While the constituents and bioactivities of EOs of the above four Chinese herbals have been reported previously, but their anti-inflammatory and antioxidant activities have not been systematically evaluated. Aim of the study The purpose of this study was to research the chemical constituents, anti-inflammatory activities, and antioxidant activities of EOs of four Chinese herbal medicines. Materials and methods Fresh plant materials were collected both in August 2018. The compositions of EOs were determined by the gas chromatography-mass spectrometry (GC-MS) method. TPA (12-O-tetradecylphophenol-13-acetate)-induced mouse ear model was evaluated the anti-inflammatory activities of EOs. The reduction of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), cyclooxygenase-2 (COX-2) and RelA protein of the nuclear factor kappa-B (NF-κB) in the signal pathway (P65) were measured. The antioxidant activities of EOs from four plants were determined by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging method. Results A total of 217 compounds were separated and identified from four EOs, mainly including trans-cinnamylaldehyde (68.75%), citronellal (38.16%), linalool (1.02–33.73%), geraniol (19.39%) and citronellol (17.18%). All four EOs reduced the damage of the ear tissue and had certain anti-inflammatory effects, and the EOs of C. citratus (CyCEOs) and S. japonica (SJEOs) had the best anti-inflammatory activities among others, even better than ibuprofen . The four EOs exhibited different DPPH radical-scavenging activities (IC 50 , 0.101–1.017%), where most of them were much lower than that of EOs of C. citratus (CiCEOs) (IC 50 , 0.101%) and EOs of R. rugosa (RREOs) (IC 50 , 0.142%). Conclusions The main components determine diverse propertise of these four EOs, our results suggested that four EOs presented anti-inflammatory and antioxidant effect via reducing the expression of TNF-a, IL-6, COX-2, and NF-κB p65, which may provide a new approach for development of new anti-inflammatory drugs.
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高影响因子
ELECTROCHEMISTRY
·
2015
·Vol. 83
·期 3
·155-160
·
IF 1.033
Yan-qing LI,Ying LUO,Li-jun LI,Hao CHENG,Wen-yi HUANG
In order to establish a simple, sensitive and rapid method for the determination of azithromycin, the gold electrode modified with Silica sol/Nano-Au/PVP/Ru(bpy)32+ was prepared, and the electrochemiluminescence (ECL) method was developed for the determination of azithromycin based on the sensitizing effect of azithromycin on Ru(bpy)32+. Moreover, the electrochemical behaviors and the electrochemical luminescence behaviors of the Ru(bpy)32+ system and the Ru(bpy)32+- azithromycin system were investigated by ECL respectively. The results show that the proposed electrode showed very good electrochemical activity and electrochemiluminescence response to the Ru(bpy)32+- azithromycin system, under the optimum experimental condition, the linearity of azithromycin concentration to ECL intensity was achieved in the concentration of azithromycin from 1.0 × 10−7 to 1.0 × 10−4 mol L−1 and the detection limit (S/N = 3) was 1.33 × 10−9 mol L−1. Through the parallel determination of 1.0 × 10−5 mol L−1 azithromycin for 8 times, the RSD of results was 1.82%, and the recovery ranging from 97.9% to 101.6% was obtained in the detection of azithromycin tabelets and the RSD was 1.36% (n = 5). The proposed method showed high selectivity and sensitivity and simplicity, and it could be applied to the analysis of azithromycin with satisfactory results.
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高影响因子
ULTRASONICS SONOCHEMISTRY
·
2022
·
IF 9.336
Ruyue Zhang,Yilong Zhang,Yating Sun,Hang Yu,Fangwei Yang,Yahui Guo,Yunfei Xie,Weirong Yao
This study evaluated how the generation of pyrazines was promoted by high-intensity ultrasound (HIU) in a Maillard reaction (MR) model system of glucose-glycine. Carbohydrate module labeling (CAMOLA) technique was adopted using D-glucose- 13 C 6 to elucidate the carbon skeleton of both intermediate and final MR products (MRPs). In the D-glucose- 13 C 6 -glycine HIU-MR model system, the concentration of 11 types of pyrazines was significantly higher than their counterparts in the thermal MR. Results of CAMOLA analysis showed that a significantly lower proportion of [M] + in pyrazines with long-length side chains was observed when compared with the pyrazines generated in thermal MR. This phenomenon may suggest the aldol-type condensation was promoted by the HIU, which is a conversion from pyrazines with short-length side chains to those with long-length side chains involving carbonyl compounds. Furthermore, the analysis of isotopomers distribution in 2,3-dimethyl-quinoxaline as the o-phenylenediamine-derivatized 2,3-butanedione indicated that the increased proportion of [M + 4] + in 2,3-dimethyl-quinoxaline (15.74% ± 0.11%) was attributed to a cleavage of D-glucose- 13 C 6 promoted by the HIU. The above-mentioned findings elucidate that the aldol-type condensation and cleavage of D-glucose contribute to the promoted synthesis of pyrazines. The HIU would generate an extremely high temperature and pressure environment that is favored by the aldol-type condensation as a high-pressure favored reaction. The HIU, therefore, can be further developed as a promising technique to promote flavor generation through the MR.
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高影响因子
FISH PHYSIOLOGY AND BIOCHEMISTRY
·
2024
·Vol. 50
·期 3
·1157-1169
·
IF 2.5
Guo Haoji,Liu Guoquan,Tian Mengdu,Liu Chunsheng,Zhang Haoran,Wang Shuqi,Wen Xiaobo,Lin Fan
Faba bean has gained attention as a cost-effective protein source with the potential to enhance product quality (texture properties, collagen content, etc.) in fish. However, its anti-nutrition factor, high feed conversion ratio, poor growth performance, etc. limit the widely application as a dietary source, especially in carnivorous fish. The water or alcohol extract of faba bean might resolve the problem. In this study, the juvenile Nibea coibor , known for their high-protein, large-sized, and high-grade swim bladder, were fed with seven isoproteic and isolipid experimental diets with the additive of faba bean water extract (1.25%, 2.5%, and 5%) or faba bean alcohol extract (0.9%, 1.8%, and 3.6%), with a control group without faba bean extract. After the 10-week feeding trail, the growth, antioxidant capacity, textural properties, and collagen deposition of the swim bladder were analyzed. Results showed that the 1.25% faba bean water extract group could significantly promote growth, textural quality of the swim bladder, and have beneficial effects on antioxidant response of fish. Conversely, dietary supplementation of faba bean alcohol extract resulted in reduced growth performance in a dose-dependent manner. Furthermore, fish fed diet with 1.25% faba bean water extract exhibited increased collagen content and upregulated collagen-related gene expression in the swim bladder, which was consistent with the Masson stain analysis for collagen fiber. Our results suggested that the anti-nutrient factor and bioactive component of faba bean may mainly be enriched in alcohol extract and water extract of faba bean, respectively. Besides, the appropriate addition of water extract of faba bean may improve the texture quality of the swim bladder by promoting collagen deposition. This study would provide a theoretical basis for the formulated diets with faba bean extract to promote product quality of marine fish.
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POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2026
·
IF 6.8
Jiahui Jiang,Qianlong Shi,Dan Li,Qingmin Chen,Cong Han,Jing Wei,Maorun Fu,Huaimei Cao,Xiaofei Xin
Vacuum-packed lotus roots slices are prone to swelling and quality deterioration when stored above 15 °C. Our study aimed to investigate the effect of combined treatment of 0.7 % lactic acid (LA), 0.7 g L −1 ε-poly- L -lysine (ε-PL), and 0.3 % sodium isoascorbate (SI) solution on the swelling, microbial growth, antioxidant and quality characteristics of vacuum-packed lotus root slices stored at 20 °C for 6 d. Results showed that, the primary bacterial genera responsible for bag swelling were identified as Tolumonas , Aeromonas , and Enterobacter, utilizing 16S rDNA amplicon sequencing. Compared with the control, the LA + ε-PL + SI treatment exhibited inhibitory effect on these genera, reduced the relative abundance of Tolumonas , Aeromonas , and Enterobacter from 65.97 %, 13.20 %, and 3.85–1.07 %, 0.20 %, and 0.44 %, respectively, yielding suppression rates of 98.4 %, 98.5 %, and 88.6 %, thus, effectively preventing swelling. Moreover, lotus root treated with LA + ε-PL + SI showed the better color, quality attributes. These findings indicated that the LA + ε-PL + SI treatment successfully prevented swelling, inhibited microbial growth, prolonged the shelf life, and preserved the better quality of vacuum-packed fresh cut lotus root.
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FOOD CONTROL
·
2026
·
IF 6.3
Li Zhou,Qingyang Zhang,Jin Zhang,Jinglun Zhou,Xinya Huang,Zhengting Zhu,Siya Zhao,Meiyu Zheng,Yong Yang,Shu Wang,Bin Li,Sihong Zhang,Dongping He,Zhigang Hu,Fenfen Lei
Sesame oil, esteemed for its robust aroma and high content of unsaturated fatty acids and micronutrients, depends predominantly on seed roasting to develop its characteristic flavor. However, this high-temperature process is associated with the risk of generating hazardous compounds, including benzo[a]pyrene (BaP), 3-monochloropropane-1,2-diol esters (3-MCPDE), and glycidyl esters (GEs), which have raised increasing regulatory concerns worldwide. In this study, an integrated approach was employed, encompassing sample morphology, physicochemical properties, levels of hazardous substances, and flavor profiles, to systematically investigate the influence of roasting conditions (180–260 °C for 10–40 minutes) on the quality, safety, and flavor of hot-water-substitution-extracted sesame oil. Results revealed that roasting induced significant microstructural degradation in the sesame seeds, darkened the oil color, and lowered the acid value, while the peroxide value was increased. Importantly, the concentrations of BaP, 3-MCPDE, and GEs exhibited a pronounced rise when roasting temperature exceeded 220 °C, establishing this threshold as a critical control point for process safety. The relative contents of polyunsaturated fatty acids, such as linoleic and linolenic acid, were slightly reduced under intensified roasting. In parallel, a concomitant increase in key volatile compounds was confirmed by electronic-nose analysis, with short-chain alkanes, aldehydes, and alcohols identified as the principal volatile substances. It was concluded that roasting conditions must be meticulously optimized to balance flavor development against the formation of processing contaminants and the degradation of nutritional components. These findings provide a crucial theoretical foundation for refining industrial sesame-oil production and for establishing science-based criteria in market quality supervision and regulatory standards.
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FOOD HYDROCOLLOIDS
·
2026
·
IF 13.8
Zhongcan Peng,Jifei Feng,Chaoqing Yang,Hong-Bin Wang,Hong-Lei Jin
Fingered citron is a pectin-rich citrus resource, but extraction of fingered citron pectin (FCP) using natural deep eutectic solvents (NADES) remains limited. This study combined conductor-like screening model for real solvents (COSMO-RS) prediction with experimental validation to screen NADES for FCP extraction. Thirty-five NADES systems were evaluated using monosaccharides and pectin fragments as molecular models, and five candidates were selected for ultrasound-assisted extraction. Betaine-maltose (Bet-Mal) achieved the highest yield (11.86%), followed by choline chloride-maltose (ChCl-Mal) (9.57%), both higher than water extraction (4.88%). Structural analyses showed that NADES changed the domain composition, molecular size, methyl esterification, surface charge, conformation, and aggregation behavior of FCPs. L-proline-maltose (LP-Mal), Bet-Mal, and choline chloride-fructose (ChCl-Fru) extracted galacturonic acid-rich pectins with high homogalacturonan proportions, whereas glucose-maltose (Glc-Mal) yielded a highly branched rhamnogalacturonan-I-rich fraction with the highest molecular weight (945.2 kDa), largest particle size, and strongest nanoscale aggregation. ChCl-Mal-extracted pectin showed the highest DPPH scavenging activity, while the water-soluble pectin (WSP) had the strongest superoxide anion scavenging activity. The κ-carrageenan composite gels prepared from pectins extracted with different solvents exhibited distinct textural, rheological, thermal, and microstructural characteristics. The LP-Mal-extracted pectin formed the strongest water-retentive gel, with hardness of 1.54 N and water holding capacity of 92.34%, whereas ChCl-Mal and ChCl-Fru showed higher small-deformation viscoelastic moduli. These results provide insight into the solvent-dependent structure–function relationships of FCP and offer a COSMO-RS-guided NADES extraction strategy for obtaining fingered citron pectins with distinct food-related functionalities.
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Changxing Wang,Feifei Qin,Siyuan Tang,Xiameng Li,Tingting Li,Guoqiang Guo,Chenjie Gu,Xu Wang,Da Chen
The design of intelligent and real-time sensing devices is significant in the medical drug monitoring field, but it is still highly challenging. Here, ratiometric fluorescent detections of ofloxacin (OFL) and its L-isomer levofloxacin (LEV) constructed from tri-doped graphene quantum dots (T-GQDs) are reported, and the detection limits reach as low as 46/67 nM toward OFL/LEV due to the intermolecular electron transfer (intermolecular ET) effect. After adding OFL/LEV, the generation of electrostatic bond provides a channel for the intermolecular ET from the edge of T-GQDs to OFL/LEV, resulting in the fluorescence quenching at 414 nm and the fluorescence promoting at 498 nm. Furthermore, a smartphone can be used for the visual and quantitative detection of OFL and LEV by identifying the RGB values of test paper and drink samples. This work not only reveals the physics mechanism of ratiometric detection, but also develops a convenient smartphone diagnostic for OFL and LEV .
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高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Meng Wang,Jingjing Wang,Kangkang Wang,Liugen Zhang,Xianglei Cao,Cheng Guo,Jide Wang,Bin Wu
More and more attention has been paid to the improved QuEChERS (quick, easy, cheap, effective, rugged and safe) method in dealing complex sample matrices, especially for the study of QuEChERS adsorbents. In this study, a magnetic mesoporous material, which was derived from MIL-88B modified by l -alanine, was synthesized as modified QuEChERS adsorbents for the simultaneous determination of multiple pesticides (Methomyl, Isoprocarb, Carbofuran, 3-Hydroxycarbofuran, Acetamiprid, Imidacloprid) in Chinese cabbage, celery, long bean and leek. The prepared magnetic adsorbents can effectively remove interfering substances from the sample, and the proposed modified QuEChERS method can reduce sample pretreatment time via an external magnetic field. To achieve the best performance of QuEChERS method, the clean-up time and amount of QuEChERS adsorbents were investigated. Under the optimized conditions, a simple, rapid and sensitive method for the determination of 6 pesticide residues in vegetables was established by coupling the modified QuEChERS to ultrahigh-performance liquid chromatography-tandem mass spectrometry. Excellent sensitivity (The limit of detection for the 6 pesticides ranged from 0.001 to 0.020 µg kg −1 ), satisfactory linearity (r 2 ≥ 0.9952), good recovery (73.9–107.7%) and good precision (3.6–16.9% for intraday relative standard deviation, 0.5–15.0% for interday relative standard deviation) were obtained. Compared with traditional QuEChERS method, the proposed method is simple, cost-effective, and efficient, which indicates that the method can be used to detect carbamate and neonicotinoid pesticides in real samples and provide an excellent pretreatment technique for the detection of trace multi-analytes from complex substrates.
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高影响因子
危险品化学品经营许可证（带存储）