Journal of Radiation Research and Applied Sciences
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2026
·Vol. 19
·期 1
·102195
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IF 2.5
Ling Li,Mingjun Zheng,Weixia Meng
Background and objective The incidence of ependymoma is higher in children than in adults, and it is a major cause of poor prognosis in pediatric patients. This study aimed to analyze the multimodal magnetic resonance imaging (MRI) characteristics of pediatric ependymoma and the correlation between programmed death-ligand 1 (PD-L1) expression in tumor tissues and clinicopathological features. Methods Fifty pediatric patients with pathologically confirmed ependymoma were enrolled. MRI, diffusion-weighted imaging (DWI), and proton magnetic resonance spectroscopy ( 1 H-MRS) features were evaluated according to WHO grading. PD-L1 positivity was detected in both lesional and adjacent normal tissues, and its relationship with clinicopathological characteristics and survival rate was analyzed. Results apparent diffusion coefficient (ADC)min, rADC, and NAA/Cr ratios were significantly lower in WHO grade III patients than in grades I, II, and II–III, while Cho/Cr was significantly higher ( P < 0.05). The PD-L1 positivity rate was significantly higher in tumor tissues (52 %) than in adjacent normal tissues (18 %) ( P < 0.05). PD-L1 expression was significantly elevated in patients with tumor diameter ≥2 cm, partial resection, moderate-to-poor differentiation, and WHO grade III ( P < 0.05). At the 10-month follow-up, the survival rate of PD-L1-positive patients (57.7 %) was significantly lower than that of PD-L1-negative patients (83.3 %) ( P < 0.05). Conclusion DWI can differentiate between ependymoma grades and improve preoperative diagnostic efficiency. Elevated PD-L1 expression in tumor tissue is associated with aggressive features and may serve as a marker for assessing short-term prognosis in pediatric ependymoma. MRI-DWI and 1 H-MRS parameters (ADCmin, rADC, NAA/Cr, Cho/Cr) reflect tumor grade and malignancy, while PD-L1 expression correlates with invasiveness and outcome. Their combination provides a more comprehensive basis for disease evaluation and prognostic assessment in pediatric ependymoma.
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BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
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2026
·Vol. 1872
·期 4
·168169
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IF 4.2
Yongjuan Wang,Xiaoli Xie,Jiajun Tian,Xiaoyu Jiang,Huiqing Jiang
Background Goosecoid (GSC) is a transcription factor implicated in epithelial-mesenchymal transition (EMT) during embryogenesis and cancer, but its function in organ fibrosis remains poorly understood. This study investigates the role and mechanistic targets of GSC in hepatic stellate cell (HSC) EMT during liver fibrogenesis. Methods We analyzed GSC and EMT marker expression in primary HSCs isolated from murine liver fibrosis models induced by carbon tetrachloride (CCl₄) or bile duct ligation (BDL), and in TGF-β-stimulated LX-2 cells. The functional role of GSC was validated via in vitro and in vivo GSC knockdown, while chromatin immunoprecipitation sequencing (ChIP-seq) combined with experimental verification was used to identify its target genes and regulatory mechanisms. Results GSC expression was significantly upregulated in activated HSCs from fibrotic mouse models and TGF-β-induced LX-2 cells, which was closely associated with enhanced EMT (downregulation of epithelial markers, upregulation of mesenchymal markers). Functional assays confirmed that GSC knockdown suppressed HSC EMT in vitro and ameliorated liver fibrosis in vivo via AAV-mediated HSC-specific GSC silencing. Mechanistically, we found that GSC undergoes increased nuclear translocation during HSC activation. ChIP-seq analysis identified Serum- and glucocorticoid-induced protein kinase 1 (SGK1) as a direct transcriptional target of GSC. Further validation revealed that SGK1, in turn, promotes HSC EMT by activating the NF-κB signaling pathway. Conclusion This study uncovers a novel mechanism by which GSC promotes HSC EMT and liver fibrosis through direct transcriptional activation of SGK1, followed by downstream NF-κB pathway activation. Summary GSC is markedly upregulated in activated HSCs from murine fibrosis models (CCl₄/BDL) and during TGF-β-induced EMT. HSC-specific GSC knockdown attenuated liver fibrosis progression. Mechanistically, GSC translocates to the nucleus where it binds the SGK1 promoter to enhance transcription, subsequently driving HSC EMT and promoting fibrogenesis through the NF-κB signaling pathway.
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高影响因子
PHYTOMEDICINE
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2026
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IF 8.3
Chong Gao,Wei Liu,Sai-Hu Liu,Shuang Zheng,Chen-Yu Wang,Xu Dai,Li-Hua Lian,Zhen-Yu Cui,Ji-Xing Nan,Yan-Ling Wu
Background Reprogramming of aerobic glycolysis occurs with HSCs activation and is associated with regression of liver fibrosis. Trigonelline (TRG), a plant alkaloid extracted from Trigonella foenum-graecum L seeds, has a variety of pharmacological effect. Purpose The current study investigated the hepatoprotective effect and mechanism of TRG against hepatic fibrosis by regulating glycolysis. Methods Anti-hepatic fibrosis effects of TRG were detected in thioacetamide (TAA)-induced hepatic fibrosis mice. HSCs or LX-2 were stimulated with TGF-β or conditioned medium (CM) from LPS-stimulated THP-1, then incubated with TRG, phloretin (PHL), rosiglitazone (RGZ), siRNA Glut-1 or plasmid with Glut-1. Results TRG significantly reduced serum transaminase levels, liver histopathological changes, excessive collagen deposition, inflammatory response, and neutrophil recruitment in TAA-induced mice. TAA increased hepatic Glut-1, HIF-1α and key enzymes of glycolysis expressions, while TRG completely reversed this effect. TRG also obviously regulated the combination of Glut-1 and HIF-1α to affect glycolysis in activated HSCs. TRG could inhibit α-SMA, IL-6, IL1R1, and HIF-1α expressions accompanying inhibition of Glut-1, function as PHL (Glut-1 inhibitor). Glut-1 silencing weakened α-SMA, IL-6, IL1R1, and HIF-1α expressions and enhanced the effect of TRG in activated LX-2. TRG inhibited LX-2 activation caused by Glut-1 overexpression, even demonstrated in interaction between LX-2 and macrophages. Further, TRG shown that improved fibrogenesis and inflammatory response by inhibiting Glut-1 compared with PHL in vivo . Conclusion TRG could ameliorate hepatic fibrosis through inhibiting ECM excessive deposition and inflammatory response. Inhibiting Glut-1-HIF-1α axis and glycolysis was a potential therapeutic strategy for TRG ameliorating liver microenvironment, further against hepatic fibrosis.
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ENVIRONMENTAL SCIENCE & TECHNOLOGY
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2026
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IF 11.3
Ruixue Meng,Yue Xiong,Meijiao Zhou,Mine Du,Lizhong Zhu,Wei Wang
Halobenzoquinones (HBQs), emerging disinfection byproducts (DBPs) in drinking water, were prioritized as potential bladder carcinogens. This study aimed to elucidate the interaction between HBQs and DNA, which may represent a critical initial molecular event in the adverse outcome pathway toward carcinogenesis. By combining high- and low-resolution mass spectrometry (MS), we developed a highly selective and sensitive analytical strategy integrating pentose neutral loss and information-dependent acquisition to characterize trace-level HBQ-DNA adducts in complex biological matrices. Deoxyguanosine (dG) was identified as the primary target of HBQs, and in vitro reaction revealed 16 mono-, di-, and trihalogenated 1:1 and 2:1 (mol:mol) adducts, including HBQ-dG, HHQ-dG, HPH-dG, and HBQ-HPH-dG (HHQ and HPH denoted halohydroquinone and halophenol, respectively). The stepwise formation involved HBQ-HHQ interconversion, cyclization of OH or halogen in HBQ with N1 and N2 in dG, and ether bond formation between HBQ and HPH. Four adducts were identified in reaction with calf thymus DNA, and in particular, 1-Cl-3-OH-PH-6,5-N1,N2-dG was identified in human urothelial cells SV-HUC-1. Molecular docking and spectroscopic analyses pinpointed intercalation and groove binding as the dominant noncovalent interactions between HBQs and DNA. This study provided the first evidence of intracellular HBQ-DNA adduct formation, linking the genotoxicity and carcinogenic relevance of HBQ DBPs.
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JOURNAL OF MOLECULAR LIQUIDS
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2017
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IF 3.648
Qingguo Zhang,Siyi Cai,Wenbo Zhang,Yalin Lan,Xinyuan Zhang
A new binary mixture system of ionic liquid 1–ethyl–3–methylimidazolium acetate ([Emim][Ac]) and methyldiethanolamine (MDEA) (IL + alkanolamine) were prepared. The physico–chemical properties of density, viscosity , electrical conductivity , and refractive index for the mixtures were determined from 288.15 K to 353.15 K. By the Vogel–Fulcher–Tamman (VFT) equation and Arrhenius equation , the temperature dependences of the viscosities and electric conductivities for the system were graphically described and the VFT equation showed better correlation for the system. To investigate the internal interactions of the mixtures, the excess volume and DFT calculations were employed. The trend of excess molar volumes showed more efficient packing structures due to the increase of interactions in the mixed system with the increasing of temperature. Further, the structures, the energetics , and charge distribution of all ions and molecules involved in interactions were estimated by DFT calculations combining the COSMO–RS methodology to describe intermolecular interactions between the [Emim][Ac] and MDEA.
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JOURNAL OF CHEMICAL THERMODYNAMICS
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2020
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IF 2.888
Dan Li,Dehui Xiao,Xiaoman Liu,Juan Zhao,Xinlu Xu,Cheng Wang,Wugang Wang
Densities and viscosities of ternary system (ethylcyclohexane + 1-butanol + ethyl octanoate) and three corresponding binary systems, (1-butanol + ethylcyclohexane), (1-butanol + ethyl octanoate), and (ethylcyclohexane + ethyl octanoate), were determined at 7 temperatures from 293.15 K to 323.15 K. For the ternary system and binary systems, the excess molar volumes ( V m E ) and viscosity deviations (Δ η ) of have been calculated. The V m E and Δ η data were fitted to the Redlich − Kister equations. The V m E values for the investigated systems are all positive, and the Δ η values are negative over the entire composition range. The density and viscosity data of blends and their corresponding excess properties or deviation may provide useful input data for thermodynamic or modelling studies.
研究领域:
化学热力学
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物理化学
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流体相平衡
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溶液化学
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化工基础数据
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FOOD RESEARCH INTERNATIONAL
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2026
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IF 8.8
Yangyu Zhou,Jiuhong Du,Huan Guo,Yanping Wu,Kai Zhong,Yue Xiao,Yina Huang,Hong Gao
A novel Huangjinya dark tea was developed via Eurotium cristatum PW-1 fermentation. • Dynamic flavor shifts were identified throughout the dark tea fermentation process. • Significant metabolic transformations occurred at stage D9, shaping dark tea quality. • Pearson correlation and molecular docking analyses unveiled key taste compounds. Motivated by the escalating prevalence of high-end dark tea products, exploration into novel tea cultivars and optimized fermentation methodologies has seen a substantial upsurge. Here, we report the first systematic application of Eurotium cristatum PW-1 fermentation to the albino tea cultivar Camellia sinensis cv. Huangjinya for dark tea production, with a core focus on delineating the quality profile of this novel Huangjinya dark tea product. We systematically delineated temporal dynamic changes in metabolite profiles and flavor characteristics throughout the entire fermentation cycle. Day 9 of fermentation (D9) was recognized as the crucial quality inflection point for the product, characterized by significant metabolite disparities, a marked elevation in theabrownins, and concomitant reductions in total phenols, flavonoids, and amino acids. Volatile compound and taste analyses suggested that E. cristatum PW-1 fermentation may contribute to a distinctive chemical profile with compounds associated with floral, sweet, and minty aromas, alongside umami and mildly astringent taste attributes. Correlation analysis and molecular docking further linked key differential metabolites to these flavor attributes, offering preliminary insights into the molecular features that may shape the unique sensory profile of E. cristatum PW-1-fermented Huangjinya dark tea. Download: Download high-res image (361KB) Download: Download full-size image
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MINERALS ENGINEERING
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2026
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IF 6
Qiuyue Sheng,Long Li,Wenjing Feng,Zhifeng Zhao,Yonghang Luo,Yuxuan Yang,Zhanglei Zhu,Ningning Zhang,Wanzhong Yin
3-TPT is used for the efficient concentration of azurite for the first time. • 3‑TPT achieves a pronounced separation efficiency between azurite and K‑feldspar. • 3-TPT chemically adsorbs onto azurite via the formation of N Cu S C rings. The strong surface hydrophilicity of azurite leads to its low recovery, and the poor flotation separation between azurite and gangue minerals further reduces the utilization efficiency of copper. This study presents a novel selective collector 1-(3-(trifluoromethyl)phenyl)thiourea (3-TPT) for the efficient recovery of azurite from K-feldspar. Results from flotation tests and contact angle measurements indicate that with 150 mg/L 3-TPT, the contact angle of azurite surface raised to 79.4°, the recovery reaches 94.16 %, while K-feldspar remains hydrophilic, with a recovery of only 11.29 %. The selective adsorption mechanism of 3-TPT on azurite surface was revealed by X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR) and Scanning Electron Microscope-Energy Dispersive Spectroscopy (SEM-EDS) analyses. XPS and FTIR analyses indicate that after the interaction of 3-TPT with the azurite surface, the C N stretching in 3-TPT exhibits a notable shift, and changes in the chemical states of Cu and S are observed during adsorption, confirming that 3-TPT chemically adsorbs onto azurite via the formation of N Cu S C rings, whereas its adsorption on K-feldspar is negligible. SEM-EDS results further confirm the selective interaction of 3-TPT on azurite surface, as evidenced by changes in surface morphology and elemental analysis. Characteristic elements S, N, and F from 3-TPT were clearly detected on the azurite surface in EDS mapping. This research highlights the potential of 3-TPT in copper concentration, not only improving the recovery efficiency of copper resources but also contributing to the advancement of more environmentally friendly direct flotation practices for copper oxide minerals. Download: Download high-res image (251KB) Download: Download full-size image
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CHEMICAL ENGINEERING JOURNAL
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2026
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IF 12.5
Na Song,Yuting Zhu,Fan He,Mengnan Zhang,Mingfu Ye,Shengjun Liu,Kui Zhang
HOF/HOF-based Z-scheme heterojunctions were successfully synthesized. • Z-scheme heterojunctions and Cu single atoms boost charge separation. • TCPP(M)-HOF/PFC-1 exhibits the highest H 2 evolution rate of 42.7 mmol g −1 h −1 . Hydrogen-bonded organic frameworks (HOFs) have exhibited extraordinarily broad application prospects in the field of photocatalysis owing to their unique structural merits. Nevertheless, pristine HOF materials generally suffer from the bottleneck of low separation efficiency for photogenerated charge carriers, which severely restricts their practical industrial applications in photocatalysis. To address this issue, herein we have successfully constructed a series of Z -scheme heterojunction photocatalysts based on the two-dimensional HOF/HOF system (denoted as TCPP (H or M)-HOF/PFC-1, where M = Cu, Zn, Ni), with the targeted composite heterostructure fabricated from TCPP(Cu)-HOF and PFC-1 HOF as the core product. Among them, the porphyrin-based TCPP(Cu)-HOF effectively broadens the spectral response range of light absorption for the composite; the precise construction of the Z -scheme heterojunction greatly enhances the separation and transport efficiency of photogenerated charges; meanwhile, the single-atom Cu sites (Cu-SA) can significantly shorten the charge migration pathway and accelerate the directional and rapid transfer of photogenerated electrons, ultimately enabling the efficient conversion of solar energy into chemical energy. Furthermore, the excellent energy level matching between TCPP(Cu)-HOF and PFC-1 HOF in the composite, coupled with the synergistic regulation of single-atom active sites, further facilitates the efficient directional migration of photogenerated charge carriers. Experimental test data confirm that the optimized TCPP(Cu)-HOF/PFC-1 heterojunction photocatalyst delivers a photocatalytic hydrogen evolution rate of up to 42.7 mmol·g −1 ·h −1 . This work fully demonstrates the outstanding advantages of HOF/HOF Z -scheme heterostructures in precisely tailoring photogenerated charge carrier dynamics, providing a novel and feasible research strategy for efficiently boosting photocatalytic hydrogen evolution performance and designing high-performance heterojunction catalysts for solar energy conversion. Here, we present a Z -scheme heterojunction photocatalyst based on two-dimensional HOF/HOF composites, specifically TCPP(M)-HOF/PFC-1 (M = Cu, Zn, Ni), with TCPP(Cu)-HOF and PFC-1 HOF as the representative system. Owing to the favorable energy level alignment between the two HOF components and the introduction of single-atom Cu sites, the directional migration of photogenerated charge carriers is significantly enhanced. Download: Download high-res image (117KB) Download: Download full-size image
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SOUTH AFRICAN JOURNAL OF BOTANY
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2026
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IF 3.1
Xian Dong Lv,Meiherinisa Maitituersun,Fei Li
This study established HPLC fingerprints of PCAE 70% and RLQ, supported by the 2020 edition of the Chinese Pharmacopeia and relevant literature, and performed network pharmacology prediction for the common constituents. • Multi-index regression analysis of uniform design data was conducted by partial least squares, by which seven active components were optimized and determined as six important active components, and their rationality was experimentally validated. • The entropy weight method was employed to unify heterogeneous data from in vitro and in vivo experiments, and the integrated important active component combination was subjected to in vivo activity validation through a mouse urinary tract infection model. • This study suggests that the active components centered on gallic acid, myricitrin, and quercitrin may constitute the major pharmacodynamic material basis of Polygonum capitatum against UTI, and preliminarily explored the underlying mechanism. Ethnopharmacological relevance As a cornerstone herb in the traditional medicine of the Miao ethnic group in Guizhou Province, Polygonum capitatum has long been used to treat diseases of the urinary system. Documented in Miao medical theory as cold in nature and bitter-pungent in taste, and as acting on the kidney and bladder meridians, it is traditionally used to clear heat, promote diuresis, relieve stranguria, and detoxify, thereby relieving pain. The aqueous extract of this herb is the sole component of Relinqing Granules (RLQ), a clinically widespread formulation. Despite its broad clinical application, the underlying material basis of its pharmacological activity remains to be fully elucidated. Materials and methods The target compounds were identified through literature investigation, and their presence in both the Polygonum capitatum 70% aqueous ethanol extract (PCAE 70%) and RLQ was subsequently verified. The common constituents shared by the two were identified by comparing the fingerprint chromatograms with reference standards, and network pharmacology was employed to predict the potential targets of these components. The constituents were then combined through uniform design to evaluate their antibacterial, anti-inflammatory, and antioxidant activities, optimized via partial least squares regression and the entropy weight method, and ultimately validated in a mouse model of UTI. Results A total of seven common constituents were identified: gallic acid, protocatechuic acid, catechin, myricitrin, quercitrin, quercetin, and kaempferol. Network pharmacology predicted that these constituents might exert anti-UTI effects through modulation of the PI3K-Akt signaling pathway. The optimal ratio combination demonstrated significant antibacterial activity against Escherichia coli and Staphylococcus aureus , while another optimal ratio enhanced catalase activity and modulated the expression of AKT-1/Nrf 2 / HO-1/NF-κB 3 in zebrafish. Following data integration using the entropy weight method, mouse validation results revealed decreased TNF-α/IL-1β levels and increased IL-10 levels, with superior efficacy compared to PCAE 70% and RLQ, without dose-dependent differences. The results implied that gallic acid, myricitrin, and quercitrin represent the potential principal active components of P. capitatum. Conclusion The optimized combination dominated by gallic acid, myricitrin, and quercitrin, identified from P. capitatum , exhibited antibacterial, anti-inflammatory, and antioxidant performance superior or equivalent to existing preparations, holding significant theoretical and practical value. Download: Download high-res image (228KB) Download: Download full-size image Graph A illustrates the experimental procedure for determining the in vitro antibacterial activity of target drugs by the MTT method; Graph B illustrates the experimental procedure for the acute toxicity assay in zebrafish; Graph C illustrates the mechanism diagram of drug administration in the zebrafish oxidative stress model; Graph D illustrates the simplified experimental procedure and mechanism diagram before drug administration in the mouse UTI model.
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BIOCHEMICAL PHARMACOLOGY
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2026
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IF 6.5
Linlin Ding,Rui He,Yongjia Hu,Jian Liu,Paizati Hamidi,Yerzati Nurman,Lei Xu,Jianfeng Wang,Xuming Deng,Aslebek Zekriya,Ying Chen,Qingjie Li
The global spread of multidrug-resistant (MDR) bacteria is devastating health systems and economies worldwide, and this critical trend has revitalized antibiotic adjuvant research. The extensive dissemination of plasmid- or chromosome-mediated resistance genes emphasizes the importance of reshaping colistin sensitivity. Here, we report the discovery that Melissa officinalis essential oil isolated from Traditional Chinese medicine, effectively potentiates the bactericidal activities of colistin against mobile colistin resistance (MCR-1) positive Escherichia coli ( E. coli ). Mechanistic analysis demonstrated that Melissa officinalis essential oil interacts with the MCR-1 protein to disrupt LPS modification, while concurrently inducing microbial iron homeostasis disruption and oxidative damage. To enhance its essential oil-mediated therapeutic potential in clinical management, Melissa officinalis essential oil-loaded nanostructured lipid carrier (MOEO-NLC) was designed with improved biocompatibility and solubility. Encouragingly, the combination of MOEO-NLC and colistin reduced bacterial burden reduction and inflammatory response suppression in a peritonitis-sepsis model. Collectively, this study advances antibacterial tactics and underscores the transformative potential of MOEO-NLC in paving the way for enduring countermeasures against antibiotic-resistant threats.
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Advanced Science
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2026
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IF 14.1
Bixiao Liu,Fuying Zhu,Zhuqing Wang,Jiawen Chen,Xiaomiao Cui,Congzhong Yang,Yao Peng,Dengyuan Feng,Li Lu,Hui Wei,Xiaozhi Zhao
Renal ischemia-reperfusion (I/R) injury, a primary cause of acute kidney injury (AKI), is driven by a self-amplifying loop of oxidative burst and immune cell infiltration. In this work, the classical herb pair Astragalus membranaceus (AM) and Angelica sinensis (AS) is employed as a composite precursor to synthesize nitrogen-rich carbon-dot nanozymes (AM-AS@CDs) via a one-step hydrothermal method. AM‑AS@CDs exhibit superoxide dismutase (SOD)-mimetic activity superior to that of CDs derived from single herbs. In vitro, AM-AS@CDs effectively alleviate oxidative stress-induced cellular damage and significantly inhibit apoptosis. In vivo, AM-AS@CDs markedly attenuate I/R-induced AKI and reduce immune cell infiltration in renal tissues. Transcriptomic analyses reveal that AM-AS@CDs downregulate the expression and phosphorylation of Fosl1 and c-Jun. Consequently, the AP-1-chemokine signaling axis is disrupted, which reduces immune cell recruitment. Furthermore, AM-AS@CDs restore redox homeostasis by upregulating antioxidant enzymes such as SOD, glutathione peroxidase 4 (GPX4), and catalase (CAT). They also attenuate the excessive activation of the Nrf2/HO-1 pathway. Overall, this precursor-formulation strategy enables precise modulation of heteroatom doping and surface chemistry in CDs. AM-AS@CDs effectively interrupt the oxidative-inflammatory positive feedback loop through dual mechanisms. These findings provide both theoretical and material foundations for the development of natural product-based nanozymes for I/R-related diseases.
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高影响因子
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