Frontiers in Microbiology
·
2025
·
IF 4.5
Lin Lin,Siyuan Li,Que Liu,Xingxing Zhang,Ying Xiong,Shaoyun Zhao,Liyue Cao,Jiaxuan Gong,Yaping Liu,Rong Wu
Objective: This study aimed to investigate the potential relation between the retarded growth of skeletal muscle (SM) and dysbiosis of gut microbiota (GM) in children with asthma, and to explore the potential action mechanisms of traditional pediatric massage (TPM) from the perspective of regulating GM and short-chain fatty acids (SCFAs) production by using an adolescent rat model of asthma.Methods: Male Sprague-Dawley rats aged 3weeks were divided randomly into the 5 groups (n=6~7) of control, ovalbumin (OVA), OVA + TPM, OVA + methylprednisolone sodium succinate (MP) and OVA + SCFAs. Pulmonary function (PF) was detected by whole body plethysmograph, including enhanced pause and minute ventilation. Airway allergic inflammation (AAI) status was assessed by concentrations of OVA-specific immunoglobulin E in plasma, interleukin (IL)-4 and IL-1β in bronchoalveolar lavage fluid via ELISA assay. SM mass was assessed by using cross-sectional areas of diaphragm muscle and gastrocnemius via hematoxylin and eosin staining. GM and SCFAs production were detected by 16S rDNA sequencing and GC-MS, respectively. The protein and gene expressions of free fatty acid receptor 2 in SM were detected by using immunohistochemical staining and qRT-PCR, respectively. qRT-PCR was used to detect other relative gene expressions that were closely related with SM mass. The activity of insulin-like growth factor-1 (IGF-1)/protein kinase B (PKB/AKT) pathway in SM was detected by western blotting test.Results: OVA exposure caused obvious AAI and poor PF in adolescent rats. OVA-exposed adolescent rats had a retarded growth of SM mass and inhibited activity of IGF-1/AKT pathway, which was related with GM dysbiosis, reduced SCFAs production and FFAR2 expressions in SM. TPM efficiently enhanced the SM mass, along with alleviating AAI and improving PF. TPM activated IGF-1/AKT pathway in SM, which was closely related with correcting GM dysbiosis, enhanced SCFAs production and FFAR2 expressions.Conclusion: The retarded growth of SM mass and inhibition of IGF-1/AKT pathway existed in OVA-exposed adolescent rats, which was related with GM dysbiosis, reduced SCFAs production and FFAR2 expressions in SM. TPM efficiently enhanced the SM mass, at least, partially via regulating GM, enhancing SCFAs production and activating FFAR2-IGF-1/AKT pathway.
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高影响因子
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
·
2024
·
IF 3.9
Ting Hao,Xin Zhao,Zhongyao Ji,Miaomiao Xia,Han Lu,Jianmin Sang,Shaowei Wang,Linxi Li,Ren-shan Ge,Qiqi Zhu
Benzophenone chemicals (BPs) have been developed to prevent the adverse effects of UV radiation and they are widely contaminated. 11β-Hydroxysteroid dehydrogenase 1 (11β-HSD1) catalyze the conversion of inactive glucocorticoid to active glucocorticoid, playing critical role in many physiological function. However, the direct effect of BPs on human, pig, rat, and mouse 11β-HSD1 remains unclear. In this study, we screened the inhibitory strength of 12 BPs on 4 species, and performed the structure-activity relationship (SAR) and in silico docking analysis. The inhibitory potency of BPs was: for human 11β-HSD1, BP6 (IC 50 = 18.76 μM) > BP8 (40.84 μM) > BP (88.89 μM) > other BPs; for pig 11β-HSD1, BP8 (45.57 μM) > BP6 (59.44 μM) > BP2 (65.12 μM) > BP (135.56 μM) > other BPs; for rat 11β-HSD1, BP7 (67.17 μM) > BP (68.83 μM) > BP8 (133.04 μM) > other BPs; and for mouse 11β-HSD1, BP8 (41.41 μM) > BP (50.61 μM) > other BPs. These BP chemicals were mixed/competitive inhibitors of these 11β-HSD1 enzymes. The 2,2′-dihydroxy substitutions in two benzene rings play a key role in enhancing the effectiveness of inhibiting 11β-HSD1, possibly via increasing hydrogen bond interactions. Docking analysis shows that these BPs bind to NADPH/glucocorticoid binding sites and forms hydrogen bonds with catalytic residues Ser and/or Tyr. In conclusion, this study demonstrates that BP chemicals can inhibit 11β-HSD1 from 4 species, and there are subtle species-dependent difference in the inhibitory strength and structural variations of BPs.
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高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2021
·Vol. 31
·期 18
·2009438
·
IF 18.808
Jianbo Yang,Zhen Xu,Jijun Wang,Ligang Gai,Xingxiang Ji,Haihui Jiang,Libin Liu
Hydrogel electrolytes have high room-temperature conductivity and can be widely used in energy storage device. However, hydrogels suffer from the inevitable freezing of water at subzero temperatures, resulting in the diminishment of their conductivity and mechanical properties. How to achieve high conductivity without sacrificing hydrogels’ flexibility at subzero temperature is an important challenge. To address this challenge, a new type of zwitterionic polymer hydrogel (polySH) electrolytes is fabricated. The anionic and cationic counterions on the polymer chains facilitate the dissociation of LiCl. The antifreezing electrolyte can be stretched to a strain of 325% and compressed to 75% at −40 °C and possesses an outstanding conductivity of 12.6 mS cm −1 at −40 °C. A direct hopping migration mechanism of hydrated lithium-ion through the channel of zwitterion groups is proposed. The polySH electrolyte-based-supercapacitor (SC) exhibits a high specific capacitance of 178 mF cm −2 at 60 °C and 134 mF cm −2 at −30 °C with a retention of 81% and 71% of the initial capacitance after 10 000 cycles, respectively. The overall merits of the electrolyte will open up a new avenue for advanced ionic conductors and energy storage device in practical applications.
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高影响因子
FEBS Open Bio
·
2025
·
IF 2.3
Wenheng Gao,Yunlong Li,Lihua Chen,Wenshuo Yang,Yong He,Ye Yang,Dengke Yin,Song Tan
Hyperlipidemia is a common chronic disease characterized by elevated levels of lipids in the blood. There is some evidence that suggests that berberine (BBR) might be beneficial for the treatment of hyperlipidemia. However, its low intestinal bioavailability limits its potential therapeutic action. In the present study, we explored the effect and the underlying mechanism of berberine–cinnamic acid co-crystal (BBR-CA), which is self-assembled from CA and BBR and displays a high intestinal bioavailability. In mice, BBR-CA showed the ability to decrease body weight gain and hepatic lipid accumulation in animals fed a high-fat diet. To further characterize the molecular basis of this effect, we established a hyperlipidemia cell model by treating human hepatocellular carcinoma cells (HepG2) with free fatty acids. Similarly to our in vivo experiments, lipid accumulation in free fatty acids-induced HepG2 cells was also reduced by BBR-CA. We hypothesized that BBR-CA might act through the regulation of sterol regulatory element-binding proteins-1 (SREBP-1), a key factor regulating lipid synthesis, and, indeed, SREBP-1 protein expression was inhibited by BBR-CA treatment, resulting in the decreased expression of its downstream proteins stearoyl-CoA desaturase 1 and acetyl-CoA carboxylase. Furthermore, the phosphorylation of phosphatidylinositol 3-kinase (PI3K), AKT and mammalian target of rapamycin (mTOR) was inhibited by BBR-CA, contributing to decreased active SREBP-1 in the nucleus, and was reversed and enhanced by the PI3K agonist recilisib and inhibitor LY294002, respectively. Taken together, our results suggest that BBR-CA could function by modulating the PI3K/AKT/mTOR signaling pathway, resulting in decreased nuclear expression of SREBP-1, as well as reduced expression of stearoyl-CoA desaturase 1 and acetyl-CoA carboxylase, thus alleviating hyperlipidemia. Further experimental validation is required to confirm these results.
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高影响因子
FUEL
·
2016
·
IF 3.611
Qi Du,Yiping Guo,Huanan Duan,Hua Li,Yujie Chen,Hezhou Liu
Hierarchical TS-1 (HTS-1) zeolite has been successfully synthesized through a novel three-step crystallization method by using a cheap and eco-friendly polymer PVA as the mesopore template. The HTS-1 particles possess an interesting stacking structure and are constructed of smaller crystals with an average diameter of 30 nm. The mesopores of HTS-1 are formed by the stacking of the smaller TS-1 nanocrystals . The catalytic results show that the HTS-1 exhibits much higher catalytic activity than conventional TS-1 in the oxidative desulfurization of dibenzothiophene (DBT) and 4,6-dimethyldibenzothiophene (4,6-DMDBT) due to the presence of intracrystal mesopores. In addition, the three-step crystallization method plays a key role in the preparation of HTS-1, which effectively solves the problem that PVA is unstable and unsuitable to be used as mesopore template under high crystallization temperature .
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Nanoscale
·
2019
·Vol. 11
·期 34
·15881-15891
·
IF 6.97
Yong Xu,Jun Chen,Ze'en Xiao,Caixia Ou,Weixia Lv,Lihong Tao,Shengwen Zhong
A porous composite electrode composed of diatomite-mixed 1,4,5,8-naphthalenetetracarboxylic dianhydride (NTCDA) is prepared by electrostatic spinning technology. Compared with traditional coated electrodes without diatomite mixing, the obtained composite electrode materials have higher porosity, larger specific surface area and faster lithium ion transport channels, which makes them exhibit better electrochemical performance, such as smaller impedance, higher capacity, and better cycling stability and rate performance. The electrospun diatomite-mixed 1,4,5,8-NTCDA composite (ED-1,4,5,8-NTCDA) electrode shows an initial coulombic efficiency of 77.2%, which is much higher than that of the electrospun 1,4,5,8-NTCA (E-1,4,5,8-NTCDA) electrode without diatomite mixing (63.8%) and the coated 1,4,5,8-NTCA (C-1,4,5,8-NTCDA) electrode (48.3%). Moreover, the ED-1,4,5,8-NTCDA electrode displays an initial discharge capacity of 1106.5 mA h g−1, which is much higher than that of the E-1,4,5,8-NTCDA electrode (546.0 mA h g−1) and the C-1,4,5,8-NTCDA electrode (185.4 mA h g−1). After 200 cycles, the capacity of the ED-1,4,5,8-NTCDA electrode remains at 1008.5 mA h g−1 with a retention ratio of 91.2%, which is also much higher than that of 753.2 mA h g−1 for the E-1,4,5,8-NTCDA electrode and 288.1 mA h g−1 for the C-1,4,5,8-NTCDA electrode. Even at a higher current density of 1500 mA g−1, its capacity remains above 508.9 mA h g−1. The ED-1,4,5,8-NTCDA electrode presents superior performance, which opens up a promising new approach for further utilization of organic materials as electrode materials in rechargeable lithium-ion batteries.
研究领域:
材料科学
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电化学
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纳米科技
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能源存储
·
功能材料
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高影响因子
JOURNAL OF CHEMICAL THERMODYNAMICS
·
2016
·
IF 2.196
Rongrong Li,Jiaqi Weng,Cuihong Ye,Anguo Ying,Qizhong Zhou,Aiguo Zhong,Hao Chen,Jianguo Yang
The equilibrium solubility of sodium 2,4-diaminobenzene sulfonate in liquid mixtures (methanol + water, isopropanol + water, and 1,2-propanediol + water) were determined at temperatures ranging from (273.15 to 323.15) K. The solubility of sodium 2,4-diaminobenzene sulfonate increased with temperature increment but decreased with the concentrations of methanol, isopropanol or 1,2-propanediol in liquid mixtures. The obtained solubility data were correlated by Apelblat equation and Sun model. On the base of the measured solubility, the dissolution enthalpy ( Δ H sol o ) of the dissolution process were calculated.
研究领域:
药物化学
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物理化学
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药剂学
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溶解行为研究
·
热力学
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高影响因子
ACS Applied Nano Materials
·
2023
·
IF 5.9
Yu Yang,Hao Cheng,Ruijue Chen,Qiying Chen,Si Gao,Li Chen,Wenyi Huang,Hongxing Kong,Lijun Li,Jun Feng
In this work, we have fabricated a surface-enhanced Raman scattering (SERS) magnetic immunosensor using a sandwich structure consisting of an SERS tag, a supporting substrate with magnetic properties, and a target. On the one hand, the SERS tag consists of AgNPs, a metal–organic framework called the zeolite imidazolate framework-67 (ZIF-67), and R6G, which acts as a Raman reporter. Due to the self-selective SERS-enhancing properties of ZIF-67 and better adsorption to analytes, SERS tags with this structure can further enhance the sensing capabilities. On the other hand, after surface functionalization with the human carboxylesterase 1 (hCE1) antibody, the nanostructure containing CoFe2O4 as the magnetic core and Ag NPs as the shell was used as the magnetic support substrate. The introduction of the CoFe2O4 magnetic core can not only realize the rapid separation of biological samples to simplify pre-treatment procedures but also stabilize and uniformly distribute the AgNPs and increase the Raman “hot spots”, thereby further enhancing the SERS intensity and improving the reproducibility. Not only that, the SERS signal can be further enhanced by the synergistic effect generated by the plasmonic coupling of the CoFe2O4 magnetic core–Ag satellite nanostructure (CoFe2O4/Ag). hCE1 in HepG-2 cell lysates and culture supernatants was rapidly determined using the constructed SERS magnetic immunosensor, and hCE1 released from acetaminophen-treated HepG-2 cells into the extracellular medium was also monitored in real time. The limit of detection was as low as 3.6 pg/mL, and the linear response covered a range of hCE1 concentrations (1.0 ng/mL–1.0 mg/mL) spanning 3 orders of magnitude. This strategy can be used for the quantitative detection of complex trace proteins or enzymes in biological samples.
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FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Yangyu Zhou,Jiuhong Du,Huan Guo,Yanping Wu,Kai Zhong,Yue Xiao,Yina Huang,Hong Gao
A novel Huangjinya dark tea was developed via Eurotium cristatum PW-1 fermentation. • Dynamic flavor shifts were identified throughout the dark tea fermentation process. • Significant metabolic transformations occurred at stage D9, shaping dark tea quality. • Pearson correlation and molecular docking analyses unveiled key taste compounds. Motivated by the escalating prevalence of high-end dark tea products, exploration into novel tea cultivars and optimized fermentation methodologies has seen a substantial upsurge. Here, we report the first systematic application of Eurotium cristatum PW-1 fermentation to the albino tea cultivar Camellia sinensis cv. Huangjinya for dark tea production, with a core focus on delineating the quality profile of this novel Huangjinya dark tea product. We systematically delineated temporal dynamic changes in metabolite profiles and flavor characteristics throughout the entire fermentation cycle. Day 9 of fermentation (D9) was recognized as the crucial quality inflection point for the product, characterized by significant metabolite disparities, a marked elevation in theabrownins, and concomitant reductions in total phenols, flavonoids, and amino acids. Volatile compound and taste analyses suggested that E. cristatum PW-1 fermentation may contribute to a distinctive chemical profile with compounds associated with floral, sweet, and minty aromas, alongside umami and mildly astringent taste attributes. Correlation analysis and molecular docking further linked key differential metabolites to these flavor attributes, offering preliminary insights into the molecular features that may shape the unique sensory profile of E. cristatum PW-1-fermented Huangjinya dark tea. Download: Download high-res image (361KB) Download: Download full-size image
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高影响因子
MINERALS ENGINEERING
·
2026
·
IF 6
Qiuyue Sheng,Long Li,Wenjing Feng,Zhifeng Zhao,Yonghang Luo,Yuxuan Yang,Zhanglei Zhu,Ningning Zhang,Wanzhong Yin
3-TPT is used for the efficient concentration of azurite for the first time. • 3‑TPT achieves a pronounced separation efficiency between azurite and K‑feldspar. • 3-TPT chemically adsorbs onto azurite via the formation of N Cu S C rings. The strong surface hydrophilicity of azurite leads to its low recovery, and the poor flotation separation between azurite and gangue minerals further reduces the utilization efficiency of copper. This study presents a novel selective collector 1-(3-(trifluoromethyl)phenyl)thiourea (3-TPT) for the efficient recovery of azurite from K-feldspar. Results from flotation tests and contact angle measurements indicate that with 150 mg/L 3-TPT, the contact angle of azurite surface raised to 79.4°, the recovery reaches 94.16 %, while K-feldspar remains hydrophilic, with a recovery of only 11.29 %. The selective adsorption mechanism of 3-TPT on azurite surface was revealed by X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR) and Scanning Electron Microscope-Energy Dispersive Spectroscopy (SEM-EDS) analyses. XPS and FTIR analyses indicate that after the interaction of 3-TPT with the azurite surface, the C N stretching in 3-TPT exhibits a notable shift, and changes in the chemical states of Cu and S are observed during adsorption, confirming that 3-TPT chemically adsorbs onto azurite via the formation of N Cu S C rings, whereas its adsorption on K-feldspar is negligible. SEM-EDS results further confirm the selective interaction of 3-TPT on azurite surface, as evidenced by changes in surface morphology and elemental analysis. Characteristic elements S, N, and F from 3-TPT were clearly detected on the azurite surface in EDS mapping. This research highlights the potential of 3-TPT in copper concentration, not only improving the recovery efficiency of copper resources but also contributing to the advancement of more environmentally friendly direct flotation practices for copper oxide minerals. Download: Download high-res image (251KB) Download: Download full-size image
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Na Song,Yuting Zhu,Fan He,Mengnan Zhang,Mingfu Ye,Shengjun Liu,Kui Zhang
HOF/HOF-based Z-scheme heterojunctions were successfully synthesized. • Z-scheme heterojunctions and Cu single atoms boost charge separation. • TCPP(M)-HOF/PFC-1 exhibits the highest H 2 evolution rate of 42.7 mmol g −1 h −1 . Hydrogen-bonded organic frameworks (HOFs) have exhibited extraordinarily broad application prospects in the field of photocatalysis owing to their unique structural merits. Nevertheless, pristine HOF materials generally suffer from the bottleneck of low separation efficiency for photogenerated charge carriers, which severely restricts their practical industrial applications in photocatalysis. To address this issue, herein we have successfully constructed a series of Z -scheme heterojunction photocatalysts based on the two-dimensional HOF/HOF system (denoted as TCPP (H or M)-HOF/PFC-1, where M = Cu, Zn, Ni), with the targeted composite heterostructure fabricated from TCPP(Cu)-HOF and PFC-1 HOF as the core product. Among them, the porphyrin-based TCPP(Cu)-HOF effectively broadens the spectral response range of light absorption for the composite; the precise construction of the Z -scheme heterojunction greatly enhances the separation and transport efficiency of photogenerated charges; meanwhile, the single-atom Cu sites (Cu-SA) can significantly shorten the charge migration pathway and accelerate the directional and rapid transfer of photogenerated electrons, ultimately enabling the efficient conversion of solar energy into chemical energy. Furthermore, the excellent energy level matching between TCPP(Cu)-HOF and PFC-1 HOF in the composite, coupled with the synergistic regulation of single-atom active sites, further facilitates the efficient directional migration of photogenerated charge carriers. Experimental test data confirm that the optimized TCPP(Cu)-HOF/PFC-1 heterojunction photocatalyst delivers a photocatalytic hydrogen evolution rate of up to 42.7 mmol·g −1 ·h −1 . This work fully demonstrates the outstanding advantages of HOF/HOF Z -scheme heterostructures in precisely tailoring photogenerated charge carrier dynamics, providing a novel and feasible research strategy for efficiently boosting photocatalytic hydrogen evolution performance and designing high-performance heterojunction catalysts for solar energy conversion. Here, we present a Z -scheme heterojunction photocatalyst based on two-dimensional HOF/HOF composites, specifically TCPP(M)-HOF/PFC-1 (M = Cu, Zn, Ni), with TCPP(Cu)-HOF and PFC-1 HOF as the representative system. Owing to the favorable energy level alignment between the two HOF components and the introduction of single-atom Cu sites, the directional migration of photogenerated charge carriers is significantly enhanced. Download: Download high-res image (117KB) Download: Download full-size image
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ACS Applied Materials & Interfaces
·
2026
·
IF 7.8
Wenyan Hao,Nan Sun,Ruifen Xue,Junkai Chang,Xiaocong Pang,Ying Zhou,Chunsheng Gao
Secondary brain injury after traumatic brain injury (TBI) is driven largely by ferroptosis-induced neuronal death and maladaptive neuroinflammation. Current therapies are limited by poor drug delivery and the narrow scope of single-pathway interventions. Here, we report a biomimetic hybrid nanovesicle (hMLV) engineered to codeliver the ferroptosis inhibitor ferrostatin-1 (Fer-1) and M2 macrophage–derived exosomes, enabling simultaneous suppression of neuronal ferroptosis and reprogramming of the immune microenvironment. The liposomal core encapsulates hydrophobic Fer-1 to enhance solubility and stability, while the exosomal membrane promotes blood–brain barrier penetration, lesion targeting via chemokine receptors, and immune evasion through CD47 expression. Within injured brain tissue, released Fer-1 restores glutathione peroxidase 4 (GPX4) activity, reduces lipid peroxidation, and prevents ferroptotic neuronal death. Concurrently, exosomal cytokines such as interleukin-10 and transforming growth factor-β drive macrophage polarization toward a reparative M2 phenotype, mitigating neuroinflammation. This dual mechanism establishes a positive therapeutic cycle: ferroptosis inhibition dampens inflammatory triggers, while M2 polarization reduces oxidative stress. In a murine TBI model, hMLV treatment conferred superior neuroprotection and functional recovery compared with monotherapies. These findings highlight hMLV as a clinically translatable nanoplatform for synergistic, mechanism-guided intervention in secondary brain injury.
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高影响因子
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