Cell Communication and Signaling
·
2025
·Vol. 23
·期 1
·1-18
·
IF 8.9
Zhang Jiaqi,Li Jinjin,Cheng Mengting,Zhou Haiyang,Zhang Xiaochen,Yan Hao,Xu Zhifei,Yang Bo,He Qiaojun,Gao Zizheng,Chen Xueqin,Luo Peihua
Sotorasib, the first FDA-approved KRAS G12C inhibitor, has demonstrated remarkable clinical efficacy in non-small cell lung cancer (NSCLC). However, its clinical utility is hampered by life-threatening interstitial lung disease (ILD), whose molecular basis remains unknown. Through the integration of transcriptomic and metabolomic analyses, we revealed that sotorasib activates PPAR-γ signaling, upregulating fatty acid oxidation (FAO) via its downstream effector CPT1B. This metabolic shift triggered mitochondrial ROS overproduction, which drives alveolar epithelial apoptosis and fibrosis in vivo and in vitro . Mechanistically, sotorasib directly binds to PPAR-γ via Thr325, facilitated by FABP4-mediated nuclear translocation, amplifying its transcriptional activity via a feedforward loop. Pharmacological inhibition of FABP4 attenuated PPAR-γ activation, mitigated ROS-driven apoptosis, and rescued pulmonary fibrosis in mice. Taken together, these findings identify the FABP4-PPARγ-CPT1b axis as a novel off-target mechanism of sotorasib-induced lung toxicity and propose FABP4 inhibition as a promising therapeutic target to enhance its clinical safety.
Section
Graphical
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2025
·
IF 3.8
Zhang Wenqi,Wang Xiaotong,Wen Shengye,Ge Wei,Deng Jianbo,Zhou Zeyang,Jiang Junhong,Yang Xiaodong,Wang Yan,Yang Shuang
Colorectal cancer (CRC) remains a global health concern, with an urgent need for better early detection and targeted therapies. Our glycoproteomics analysis comparing CRC to paracancerous (PCA) tissue revealed significant alterations. Global proteomic analysis showed more unique proteins in CRC, with differential expression across CRC stages, especially in protein synthesis, translation, and degradation pathways. Despite overall proteomic differences, intact glycopeptide analysis showed high glycosylation similarity between CRC and PCA, with 722 shared glycosites, 2102 shared glycopeptides, and 435 shared glycoproteins. However, a significant shift in CRC’s N -glycan composition was observed: an upregulation of high-mannose N -glycans and a decrease in complex N -glycans, including sialoglycans and fucosylated sialoglycans. While glycosylation motif analysis showed conserved peptide sequences, the attached N -glycan types differed remarkably. KEGG pathway analysis suggested that extracellular matrix and lysosomal glycoproteins like COL1A1 and LAMP1 might modulate the PI3K/Akt signaling pathway via TGFβ1-mediated mechanisms, potentially contributing to tumor progression. These findings suggest that while global protein expression changes significantly in CRC, subtle but crucial changes in N -glycan composition, specifically the increase of high-mannose N -glycans and the decrease of complex N -glycans, may play a significant role in tumor progression and metastasis, potentially through modulation of key signaling pathways. Graphical
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2025
·Vol. 16
·期 1
·1-10
·
IF 15.7
Zhang Daoyong,Wu Tao,Li Biao,Ding Degong,Li Ruilin,Wei Jiyao,Zhang Hengyu,Kan Chenxia,Yao Yuxin,Hang Pengjie,Yu Zhiguo,Ni Zhenyi,Qiu Kaifu,Yang Deren,Yu Xuegong
The pursuit of higher-efficiency solar cells has spurred the integration of perovskite materials with silicon-based technologies, yet achieving an efficient tandem architecture that leverages industrially textured silicon (ITS) with pyramid sizes larger than 2 μm remains a significant challenge. Such textured surfaces complicate the uniform coverage of the subsequent hole-selective layer deposition and the high-quality deposition of perovskites, ultimately causing significant contact losses in tandem devices. This study presents a tandem solar cell architecture that employs localized submicron contacts, enabled by silica (SiO X ) nanospheres, to effectively regulate silicon substrate surfaces that exhibit iceberg-like pyramids. This architecture facilitates the complete coverage of solution-processed perovskites on ITS substrates while substantially reducing interfacial recombination losses and enhancing charge carrier transport. Consequently, the developed tandem solar cells demonstrate certified power conversion efficiencies of up to 33.15% in a one square centimeter area, along with improved device stability.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2015
·Vol. 407
·期 20
·6071-6079
·
IF 3.436
Du Fuyou,Sun Lin,Zhen Xian,Nie Honggang,Zheng Yanjie,Ruan Guihua,Li Jianping
High-internal-phase-emulsion polymers (polyHIPEs) show great promise as solid-phase-extraction (SPE) materials because of the tremendous porosity and highly interconnected framework afforded by the high-internal-phase-emulsion (HIPE) technique. In this work, polyHIPE monolithic columns as novel SPE materials were prepared and applied to trace enrichment of cytokinins (CKs) from complex plant samples. The polyHIPE monoliths were synthesized via the in-situ polymerization of the continuous phase of a HIPE containing styrene (STY) and divinylbenzene (DVB) in a stainless column, and revealed highly efficient and selective enrichment ability for aromatic compounds. Under the optimized experimental conditions, a method using a monolithic polyHIPE column combined with liquid chromatography–electrospray tandem mass spectrometry (LC–MS–MS) was developed for the simultaneous extraction and sensitive determination of trans -zeatin ( t Z), meta -topolin ( m T), kinetin (K), and kinetin riboside (KR). The proposed method had good linearity, with correlation coefficients ( R
2 ) from 0.9957 to 0.9984, and low detection limits (LODs, S/N = 3) in the range 2.4–47 pg mL −1 for the four CKs. The method was successfully applied to the determination of CKs in real plant samples, and obtained good recoveries ranging from 68.8 % to 103.0 % and relative standard deviations (RSDs) lower than 16 %.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 7
·1-12
·
IF 5.3
Wu Han,Li Hai,Shu Jiayi,Yang Changyan,Wang Jianglong,Li Haitao,Shao Min,Zhou Hefeng
In December 2019, Wuhan, China, reported the first cases of pneumonia caused by a previously unknown coronavirus, subsequently designated SARS-CoV-2. This pathogen precipitated a global pandemic of coronavirus disease 2019 (COVID-19). The virus primarily transmits via aerosols, direct contact, and contaminated surfaces. Its high infectivity and rapid transmission have posed severe public health challenges on a global scale, emphasizing the essential need for precise and swift diagnostic techniques to effectively track and curb its transmission. To address this need, we designed a highly efficient approach, namely a colloidal gold immunochromatographic strip (CGICS) based on an IgG-mediated immunoassay, for the specific capture of the SARS-CoV-2 nucleocapsid (N) antigen. Evaluation of the assay’s specificity with a panel of common respiratory viral pathogens revealed that the CGICS specifically recognized SARS-CoV-2, with no cross-reactivity observed against non-target viruses. In addition, limit-of-detection (LOD) assessments indicated that the minimum detectable concentration was 2 ng/mL, and agreement analysis experiments showed a concordance rate of 98%, demonstrating high specificity and sensitivity. The resulting CGICS was capable of detecting SARS-CoV-2 antigen within 5–15 min. This study provides a rapid diagnostic approach for early SARS-CoV-2 infection, offering significant implications for effective disease prevention, control, and clinical diagnosis. Graphical
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOLOGICAL TRACE ELEMENT RESEARCH
·
2025
·
IF 3.6
Li Jianchao,Deng Nuo,Wang Fei,Zhu Huilin,Li Yongming,Wu Xiao
As a traditional Chinese medicine (TCM) composed of As 2 S 2 , realgar has been widely used for treating various diseases. However, its safety has raised significant concerns due to the well-documented toxicity of arsenic. The Chinese Pharmacopoeia has imposed strict controls on the dosage of realgar, but the bioaccessibility and health risks associated with arsenic in realgar-containing TCMs remain poorly understood. This study developed a hydride generation–atomic fluorescence spectrometry (HG-AFS) method to determine the total arsenic and bioaccessible arsenic contents in 20 realgar-containing TCMs through wet digestion and simulated gastrointestinal fluid extraction. Health risk assessments were evaluated from the bioaccessible arsenic content using single pollution index (Pi), maximum daily intake (MDI), estimated daily intake (EDI), hazard quotient (HQ), and carcinogenic risk (CR). Additionally, the particle size of realgar in representative preparations was analyzed using a light microscope. The tested realgar-containing TCMs exhibited significant variations in the contents of total arsenic and bioaccessible arsenic. Bioaccessible arsenic is a more reasonable indicator for the safety evaluation of realgar-containing TCMs. The daily dosage of realgar in its-containing TCMs exceeded the limit standard prescribed in the Chinese Pharmacopoeia. The evaluation of the health risk index suggested that arsenic in realgar-containing TCMs exceeded the corresponding regulatory thresholds. Furthermore, realgar crystals in preparations were not uniform and exhibited noticeable differences under the microscope. The current quality standards in the Chinese Pharmacopoeia are insufficient to ensure the safety of realgar-containing TCMs, which urgently need to be revised. The study provided scientific evidence for the quality control and clinical safety application of realgar-containing TCMs. Graphical
✓ 已验证
产品关联
DOI
PubMed
高影响因子
WATER AIR AND SOIL POLLUTION
·
2025
·Vol. 236
·期 9
·1-16
·
IF 3
Li Yawen,Ma Guoyang,Xu Shaohong,Farooq Usman
This study quantitatively investigated the inhibitory effect of cysteine (CYS), an eco-friendly compound, on the degradation efficiency of trichloroethylene (TCE) in heterogeneous nano zero-valent iron (nZVI)-activated percarbonate (SPC) systems. The addition of CYS to the nZVI/SPC system reduced the TCE oxidation capability from 90.5% to 81.3%, whereas Fe(II)/SPC exhibited an increase in oxidation capability. Increasing CYS concentration from 5 to 20 mM decreased TCE degradation from 86.7% to 68.5%. The presence of thiol (-SH) groups in CYS led to the formation of disulfide bonds with iron nanoparticles, effectively blocking their surface and inhibiting their interactions with contaminants. Theoretical calculations using first principles confirmed that CYS had the highest reaction energy barrier with •OH (0.50 eV), indicating a minimal impact on TCE degradation efficiency. Furthermore, -SH acted as an antioxidant, scavenging free radicals and limiting the participation of nZVI in the Fenton-like oxidation process. The conversion of CYS to cystine was observed, and FTIR analysis revealed modifications in the nZVI structure due to -SH group reactions. The disappearance of specific peaks indicated the blockage of active surface sites on nZVI, further inhibiting TCE degradation. These computational discoveries substantiate the experimental findings and provide additional insights into the interactions of SH-containing organics with nZVI in groundwater remediation technologies.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2025
·Vol. 23
·期 1
·1-23
·
IF 12.6
Qiao Yang,Liu Menghui,Zhang Yiqun,Ni Fan,Yu Liangchen,Chen Zhao,Dai Xingliang,Wang Xianwen
The complex dual role of autophagy provides new insights for enhancing tumor treatment efficacy. However, effectively regulating this process is key to enhancing therapeutic efficacy. To address this challenge, this study designed a gambogic acid-iron nanozyme (GAFe) as a novel carrier to enhance the effectiveness of chemotherapy drugs such as doxorubicin (DOX) by inducing excessive autophagy and oxidative stress. The synthesized nanoparticle (GAFe@DOX) is capable of slowly releasing its active components over a prolonged period within tumor tissues. Gambogic acid can induce excessive autophagy, while the multi-enzyme activity of GAFe and the activation of ferroptosis amplify and sustain excessive autophagy, thereby enhancing the chemotherapy effect of DOX. Meanwhile, ferroptosis activated via the GPX4 pathway by GAFe can synergize with excessive autophagy, amplifying oxidative stress and consequently enhancing the overall therapeutic efficacy. Characterization experiments confirmed the successful synthesis of GAFe@DOX and probe assays demonstrated its superior multi-enzyme activity. In vitro cell studies showed that GAFe@DOX effectively kills tumor cells, while in vivo animal experiments revealed its excellent biocompatibility and significant tumor growth inhibition. This study demonstrates a promising strategy to improve tumor therapeutic efficacy by modulating excessive autophagy and oxidative stress. This provides a novel and effective approach to improve the treatment of refractory tumors such as glioblastoma. Graphical abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2025
·Vol. 36
·期 16
·1-18
·
IF 2.8
Su Shen,Zhang Ying,Zhang Yang,Yang Shuangshuang,Wang Xingwei,Li Leilei,Yu Qiangliang
Currently, most aerogel preparation is prepared by suspension lyophilization and high-temperature carbonization. Such preparation methods are complicated and difficult to control. In response to this problem, this study adopted silane coupling agent KH570 to modify carbon nanotubes (CNTs) and improve its dispersion stability. Modified CNTs were introduced into the acrylic/acrylamide prepolymerization system, and a three-dimensional network hydrogel was constructed through free radical polymerization and crosslinking. Ultralight copolymer aerogels (density 0.10766 g/cm 3 ) were prepared in combination with a freeze-drying process. BET analysis shows that the pore wall is rich in mesoporous, providing the basis for electromagnetic wave multiple reflections and interface polarization losses. By optimizing the CNTs addition, it was found that at 5% content, the aerogel achieves excellent performance at an ultra-thin thickness of 1.15 mm: the minimum reflection loss is − 54.8 dB, the effective absorption bandwidth is up to 4.13 GHz (13.87–18 GHz), effectively improving the height of traditional materials The problems of density and narrow bandwidth meet the needs of "light, thin, wide and strong" wave absorption. Its low-temperature environmental protection technology is both scalable, providing new strategies for the development of high-performance wave-absorbing materials in the fields of 5G communications, military stealth, etc., and has important application value.
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 7
·1-9
·
IF 5.3
Yao Zimin,Zheng Guoliang,Zheng Yonggui,Wen Xiaokun,Hong Xia,Yan Jiayi,Liu Wen,Wang Jiwei,Wang Kexin,Cao Shuo
Biomolecular detection based on a single fluorescence signal response is often difficult to completely eliminate the influence from interfering factors on the assay results. In this work, a ratiometric fluorescence probe was designed based on a dual emission carbon dot (CD). It produces dual-color emission (400 nm blue emission band and 560 nm green emission band) under 360 nm excitation. The two emission bands are believed to be originated from the distinct luminescent mechanisms: the intrinsic emission of CDs and intramolecular charge transfer (ICT) process, respectively. The energy transfer between the dual emission leads to the differentiated responses towards sialic acid (SA). As the concentration of SA increases, the two emission bands of CDs dispersion exhibit opposite variation trends. The blue emission intensity rose slightly and green emission intensity descended. The ratios of emission band intensity at 400 nm and 560 nm are served as quantitative assay of SA. The limit of detection reached 1.15 μM. The ratiometric fluorescence probe also shows a favorable selectivity for SA detection. The self-calibration and mutual verification functions of the probes enable consistent emission ratios across different models of commercial spectrometers. It minimizes the interference from the environment and instrumentation, achieving accurate and highly versatile SA detection. The utility of the probe for SA detection was verified by analyzing human serum, showing the tremendous potential of dual emission CDs for accurate SA detection. Graphical
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL GEOCHEMISTRY AND HEALTH
·
2025
·Vol. 47
·期 7
·1-18
·
IF 3.8
Liu Haicheng,Rao Chenxuan,Xu Huixuan
This study investigated the effects of different aging treatments (UV light, potassium permanganate, and sodium hydroxide treatments) on the adsorption of lead (Pb(II)) by PET microplastics, as well as the impact of Microcystis aeruginosa on lead adsorption by PET microplastics. The results indicated that different aging treatments significantly affected the adsorption capacity of microplastics, which could be attributed to the increased functional groups and surface porosity during the aging process. Kinetic analysis revealed that the adsorption of Pb(II) follows a pseudo-second-order model, suggested that the adsorption process is mainly controlled by chemical adsorption. Intraparticle diffusion analysis further elucidated the migration mechanism of Pb(II) within the microplastics. On the other hand, the presence of Microcystis aeruginosa significantly enhanced the adsorption capacity of microplastics. As the concentration of microalgae increases, the promoting effect of microalgae on Pb(II) adsorption by microplastics gradually strengthens, with extracellular polymeric substances (EPS) secreted by microalgae playing a key role in facilitating Pb(II) adsorption. These findings suggested that microalgae could strengthen the role of microplastics as carriers of heavy metal lead and aggravate the risk of lead pollution diffusion in aquatic ecological environment.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Food Measurement and Characterization
·
2026
·
IF 3.6
Jia Ziyu,Zeng Xintang,Zhayitijiang Zilalan,Ma Bohan,Zou Xianfeng
This research involved preparing soy protein isolate (SPI) and xanthan gum (XG) conjugates (SPI-XG con) through the Maillard reaction (MR). The effects of temperature and time on Maillard reaction products (SPI-XG con) were evaluated, with degree of grafting (DG), intermediate products, browning degree, emulsifying activity index (EAI), and emulsifying stability index (ESI) serving as the key indicators. Results indicated that the optimal MR conditions were 90 °C and 1.5 h. Under these conditions, DG reached a maximum of 24.81%. Compared with SPI, the performance of the optimized conjugate was enhanced by 554.47% and 461.87% in EAI and ESI, respectively. MR could improve the emulsifying property of the conjugate and further enhance emulsion stability. Characterization techniques, including Fourier transform infrared spectroscopy, polyacrylamide gel electrophoresis, and protein secondary structure analysis, also verified the formation of SPI-XG con. Changes in the morphology of SPI following the MR were observed via scanning electron microscopy. Water-in-oil-in-water (W/O/W) emulsions were prepared with an optimized conjugate. The W/O/W emulsions stabilized with conjugate showed smaller droplets size and higher apparent viscosity. The present study explored the possibility of obtaining W/O/W emulsions by using SPI-XG con prepared via MR, and a theoretical application for further study was also made.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）