JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
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2020
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IF 1.992
Jin Xu,Xuejia Shi,Xiaomeng Zhang,Zhenzhong Wang,Wei Xiao,Linguo Zhao
To efficiently recycle GH78 thermostable rhamnosidase (TpeRha) and easily separate it from the reaction mixture and furtherly improve the enzyme properties, the magnetic particle Fe 3 O 4 -SiO 2 -NH 2 -Cellu-ZIF8 (FSNcZ8) was prepared by modifying Fe 3 O 4 -NH 2 with tetraethyl silicate (TEOS), microcrystalline cellulose and zinc nitrate hexahydrate. FSNcZ8 displayed better magnetic stability and higher-temperature stability than unmodified Fe 3 O 4 -NH 2 (FN), and it was used to adsorb and immobilize TpeRha from Thermotoga petrophilea 13995. As for properties, FSNcZ8-TpeRha showed optimal reaction temperature and pH of 90°C and 5.0, while its highest activity approached 714 U/g. In addition, FSNcZ8-TpeRha had better higher-temperature stability than FN. After incubation at 80°C for 3 h, the residual enzyme activities of FSNcZ8-TpeRha, FN-TpeRha and free enzyme were 93.5%, 63.32%, and 62.77%, respectively. The organic solvent tolerance and the monosaccharides tolerance of FSNcZ8-TpeRha, compared with free TpeRha, were greatly improved. Using naringin (1 mmol/l) as the substrate, the optimal conversion conditions were as follows: FSNcZ8-TpeRha concentration was 6 U/ml; induction temperature was 80°C; the pH was 5.5; induction time was 30 min, and the yield of products was the same as free enzyme. After repeating the reaction 10 times, the conversion of naringin remained above 80%, showing great improvement of the catalytic efficiency and repeated utilization of the immobilized α-L-rhamnosidase.
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高影响因子
MICROCHEMICAL JOURNAL
·
2019
·
IF 3.206
Qiqi Tan,Juan Qiao,Rongyue Zhang,Li Qi
Papaya juice protected copper nanoclusters was depicted for detection of serum l -histidine. The fluorescent probe was synthesized by using a simple “one pot” hydrothermal preparation strategy with papaya juice playing dual roles both as a green reductant and stabilizer. The excitation and emission peaks of the copper nanoclusters were at 400 nm and 490 nm, respectively. The fluorescence was turned-on in addition of l -histidine due to the enhanced surface electron density. The fluorescent intensity increased linearly with l -histidine concentration ranging from 2.0 to 50.0 µM. The proposed copper nanoclusters was further applied to determine l -histidine in rat serum after intraperitoneal injection.
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EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
·
2018
·
IF 3.466
Longyi Nan,Chao Liu,Qiaoyun Li,Xiaocao Wan,Jianpeng Guo,Peng Quan,Liang Fang
It has been reported that natural transdermal permeation enhancers (TPEs) are superior in safety compared with synthetic TPEs. The essential oil (EO) of Ledum palustre L. var. angustum N. Busch had a strong enhancement effect on drug skin permeation based on previous studies. However, their enhancement mechanisms and safety were still unclear. The composition of the EO was determined using GC–MS. By using donepezil (DNP) as a model drug, the enhancement effect of the constituents of the EO and the EO were evaluated by in vitro skin permeation test. Confocal laser scanning microscopy (CLSM), attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) and molecular docking were used to investigate the interaction among drug, enhancers and skin. Skin retention amount, apparent partition coefficient ( K′ ) and molecular simulation were used to reflect the effect of the enhancers on drug partition into skin. The skin irritation potential was evaluated using in vivo skin erythema analysis. The results showed that the main constituents of the EO were sabinene (SA), 4-terpineol (TE), p-cymene (CY) and cuminaldehyde (CU). CU was the main active constituent of the EO, which facilitated skin permeation of DNP. CU improved the skin permeation of DNP by increasing the mobility of the stratum corneum (SC) intercellular lipids, decreasing the interaction between DNP and the SC intercellular lipids, and improving the partition of DNP into the SC layer. Besides the superior enhancement effect, CU also showed a lower skin irritation potential compared with the EO. This work gave us some enlightenment that the effectiveness and safety of the natural transdermal permeation enhancers could be improved by understanding their composition and the enhancement mechanisms.
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CARBON
·
2023
·
IF 10.9
Linzi Huang,Jiaodi Li,Yilin Wang,Xuan Hu,Kai Shi,Chunyan Li,Yixi Xie,Pengcheng Zhao,Junjie Fei
l -cysteine (L-Cys) plays an important role in human health as well as in the biological environment. Here, a simple physical mixing technique was used to create the p-n heterojunction by homogeneously immobilizing cuprous oxide/copper@microporous carbon (Cu 2 O/Cu@mC) on the surface of a sheet of g-C 3 N 4 (CNS). Among them, the porous structure of Cu 2 O/Cu@mC after heat treatment is favorable for charge storage. Meanwhile, the carbon element in Cu 2 O/Cu@mC acts as a photosensitizer and electron mediator to extend visible light absorption. Compared with bulk g-C 3 N 4 (CNB), the sheet-like structure and high specific surface area of CNS can be used as a carrier for the binding of Cu 2 O/Cu@mC with L-Cys. Based on the interaction between L-Cys and Cu 2 O/Cu@mC through the formation of Cu–S bonds, Cu 2 O/Cu@mC/CNS was proposed as a light-driven enzyme-free photoelectrochemical sensor for the detection of L-Cys. In the best case, the Cu 2 O/Cu@mC/GCE sensor has a low limit of detection of 1.26 × 10 −9 M for L-Cys and a wide linear range (1.0 × 10 −8 M − 4.0 × 10 −6 M). The repeatability, reproducibility, stability, selectivity and specificity of the sensor were satisfactory. In addition, the potential applicability of the proposed photoelectrochemical sensor was evaluated in tap water and human serum.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2023
·
IF 3.4
Mingxia Liu,Jing He,Canjun Ruan,Weigang Pan,Peixian Mao,Zuoli Sun,Gang Wang,Jian Yang
D -amino acids may be indicators of late-life depression but separation and quantification of enantiomers which differ only by optical rotation sign remain challenging due to their identical physical and chemical properties. A convenient LC-MS/MS method was developed for the simultaneous measurement of l - and d -amino acids based on the chiral derivatization reagent, N α -(5-fluoro-2,4-dinitrophenyl)- L -leucinamide, and conventional octadecylsilane reversed-phase column. Methanol was used as the extraction solvent and a single-step derivatization reaction using volatile triethylamine eliminated the requirement for desalination prior to LC-MS/MS. Simultaneous separation and identification of 21 amino acids and the enantiomeric compositions of the 18 chiral proteogenic entities were achieved. Low limits of detection (0.03–4.0 nM), wide linear range (0.01–20 μM), good precision (RSDs < 10 %) and negligible matrix effects indicated the suitability of the method. Application of the method to the quantification of serum chiral amino acids in late-life depression patients (n = 40) and controls (n = 35) found a total of 17 L -amino acids, 14 D -amino acids, D L -asparagine, glycine and γ-aminobutyric acid. The statistical evaluation showed significant differences of glycine, L -threonine and D -methionine between late-life depression patients and controls, indicating that these are potential biomarkers of late-life depression.
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ANALYTICA CHIMICA ACTA
·
2018
·
IF 5.123
Shan Huang,Erli Yang,Jiandong Yao,Yi Liu,Qi Xiao
Herein, a simple and efficient fluorescent assay for Ag + ions and l -cysteine (L-Cys) in complex biological fluids and living cells was first developed based on the fluorescent “on-off-on” mode of red emission nitrogen, boron, sulfur co-doped carbon dots (NBS-CDs). Red emission NBS-CDs were prepared via one-step hydrothermal synthesis by using 3-aminobenzeneboronic acid and 2,5-diaminobenzenesulfonic acid as precursors. Such NBS-CDs exhibited excellent optical properties and relatively high absolute fluorescent quantum yield compared with some reported NBS-CDs. Due to the strong quenching ability of Ag + ions on the fluorescence of NBS-CDs, red emission NBS-CDs were used for the determination of Ag + ions with high sensitivity and excellent selectivity . The fluorescence of NBS-CDs was recovered after the interaction between Ag + ions and L-Cys, which realized the specific determination of L-Cys in human urine samples and human plasma samples. The established NBS-CDs-based fluorescent “on-off-on” sensor offered a relatively low detection limits of 0.35 μM for Ag + ions and 0.045 μM for L-Cys based on three times signal-to-noise criteria. Notably, this strategy was applied for the visual detections of Ag + ions and L-Cys in living human cancer cells (HeLa cells and MCF-7 cells). This method is simple, high sensitive, and excellent selectivity, which provided a new insight on the potential applications of NBS-CDs to develop the biosensor in clinical diagnosis and other biologically related areas.
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FOOD CHEMISTRY
·
2016
·
IF 4.052
Qiang Xia,Liping Wang,Congcong Xu,Jun Mei,Yunfei Li
The effects of germination and high hydrostatic pressure (HHP) processing on the in vitro bioaccessibility of mineral elements, amino acids (AAs), antioxidants and starch in brown rice (BR) were investigated. Germinated BR (GBR) was obtained by incubating at 37 °C for 36 h and then subjected to HHP treatments at 0.1, 100, 300 and 500 MPa for 10 min. The in vitro bioaccessibility of calcium and copper was increased by 12.59–52.17% and 2.87–23.06% after HHP, respectively, but bioaccessible iron was decreased. In addition, HHP significantly improved individual AAs, particularly indispensable AAs and gama-aminobutyric acid, as well as bioaccessible total antioxidant activities and starch resistance to enzymatic hydrolysis. However, germination greatly increased starch digestibility. Atomic force microscopy characterization suggested an obvious structural change in bran fraction at pressures above 300 MPa. These results can help to understand the effects of germination and HHP technologies on nutrients bioaccessibility and develop appropriate processing conditions.
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International Journal of Nanomedicine
·
2017
·
IF 4.3
Fang Shan,YuJuan Liu,Haiying Jiang,Fei Tong
Here, we describe a bone morphogenetic protein-2 (BMP-2) nanocarrier based on glycyrrhetinic acid (GA)-poly(ethylene glycol) (PEG)-b-poly( l -lysine) (PLL). A protein nanocarrier was synthesized, characterized and evaluated as a BMP-2 delivery system. The designed nanocarrier was synthesized based on the ring-opening polymerization of amino acid N-carboxyanhydride. The final product was measured with 1 H nuclear magnetic resonance. GA-PEG-b-PLL nanocarrier could combine with BMP-2 through electrostatic interaction to form polyion complex (PIC) micelles. BMP-2 could be rapidly and efficiently encapsulated through the GA-PEG-b-PLL nanocarrier under physiological conditions, exhibiting efficient encapsulation and sustained release. In addition, the GA-PEG-b-PLL-mediated BMP-2 delivery system could target the liver against hepatic diseases as it has GA-binding receptors. The anti-hepatic ischemia/reperfusion injury (anti-HI/RI) effect of BMP-2/GA-PEG-b-PLL PIC micelles was investigated in rats using free BMP-2 and BMP-2/PEG-b-PLL PIC micelles as controls, and the results showed that BMP-2/GA-PEG-b-PLL PIC micelles indicated significantly enhanced anti-HI/RI property compared to BMP-2 and BMP-2/PEG-b-PLL. All results suggested that GA-PEG-b-PLL could be used as a potential BMP-2 nanocarrier.
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Antioxidants
·
2024
·Vol. 13
·期 11
·1291
·
IF 6
Xinyao Li,Hao Ling,Zengyang He,Zihui Yang,Tao Jiang,Peng Huang,Jianguo Zeng
The hybrid plant edible grass (Rumex patientiaL. ×Rumex tianschanicusA. LOS), a member of the Rumex genus, presents a novel food source with a protein content of approximately 30–40%. By incorporating non-traditional feed sources, such as edible grass leaf powder (EGLP), into broiler production, costs could be reduced. The experimental subjects of this study were Arbor Acres (AA) broilers. A total of 300 newly hatched broilers were randomly divided into to five groups, with each group consisting of six cages, housing 10 birds per cage. The control group was fed a basal diet composed of maize and soybean meal. In the experimental groups, varying percentages (3%, 6%, 9%, and 12%) of EGLP were substituted for the corn–soybean meal in the basal diet. In the diet, for days 1–21, the corn content was reduced from 1.90% to 8.20%, and the soybean meal content was lowered from 1.50% to 6.00%. Similarly, in the diet, for days 22–42, the corn content was decreased from 1.17% to 7.00%, while the soybean meal content was reduced by 1.50% to 6.00%. The experiment lasted 42 days and was divided into two phases: the brooding phase (days 1 to 21) and the finishing phase (days 22 to 42). The results show that substituting 3% and 6% EGLP for corn and soybean meal significantly increased the average daily gain (ADG) during the brooding period compared to the control group (p< 0.01). Additionally, the group with a 3% substitution rate exhibited a significant increase in the average daily feed intake (ADFI) during the brooding phase (p< 0.01). Throughout the 42-day experiment, substituting 3% and 6% of EGLP for maize and soybean meal did not significantly affect the overall growth performance of broilers (p> 0.05). However, a 6% supplementation notably reduced the feed conversion ratio (FCR) (p< 0.01). Both the 3% (EG3) EGLP and 6% (EG6) EGLP replacement meals significantly enhanced the antioxidant capacity of broiler chickens, as indicated by the increased levels of the total antioxidant capacity (T-AOC), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) (p< 0.01). Additionally, broilers supplemented with 3–12% showed a marked increase in cecal short-chain fatty acids (SCFAs) compared to the control group (p< 0.01). The 3% EGLP replacement diet also significantly boosted the prevalence ofLactobacillusin the cecum (p< 0.01). Furthermore, after EGLP supplementation, there was a higher abundance of SCFA-metabolizing bacteria, particularlyAlistipesandChristensenellaceae_R-7, compared to pre-treatment (p< 0.05). The prevalence ofClostridiumwas significantly greater in the groups receiving 9% and 12% EGLP (p< 0.05), whileButyrivibriolevels were notably higher after supplementation with 12% EGLP (p< 0.05).
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Nan Yao,Shifeng Ji,Yunqing Xing,Jinchao Chen,Yanrui Cheng,Xiaonan Ruan,Jiaxin Chen,Zhuo Li,Yingjie Zhang,Chunmei Gao,Changbing Ye
This study reports the development and performance of a chlorine-resistant, negatively charged loose nanofiltration (LNF) membrane designed for efficient dye/salt separation from textile wastewater. The membrane was constructed by incorporating a synergistic composite of zeolitic imidazolate framework-L (ZIF-L) and polydopamine (PDA) into a polyamide layer on a polyethersulfone (PES) support via interfacial polymerization. In this design, ZIF-L nanosheets create a highly permeable, loose network, while PDA acts as a crucial molecular linker to ensure homogeneous dispersion and structural integration. Characterization using SEM, XRD, FTIR, XPS, and AFM confirmed successful synthesis. The optimal membrane, loaded with 2 wt% ZIF-L/PDA, exhibited superior performance: a pure water flux of 31.56 L·m −2 ·h −1 ·bar −1 , which is 3.19 times greater than the pristine Thin-Film Composite (TFC) membrane. This membrane achieved high dye rejection rates: Eriochrome Black T (99.25 %), Acid Chrome Blue K (97.87 %), Direct Blue 71 (97.56 %), Congo Red (94.09 %), and Direct Red 23 (93.92 %). Simultaneously, it showed low rejection for salts, indicating high permeability: NaCl (7.80 %), MgCl 2 (9.89 %), MgSO 4 (15.39 %), and Na 2 SO 4 (23.97 %). Crucially, the membrane demonstrated robust stability under diverse and harsh conditions, including exposure to strong acid, strong alkali, high pressure, and during long-term continuous operation, along with markedly enhanced chlorine resistance. Therefore, the ZIF-L/PDA loose NF membrane presents significant potential for dye wastewater treatment applications.
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Food and Bioprocess Technology
·
2018
·Vol. 11
·期 10
·1853-1862
·
IF 2.998
Sun Zhidong,Hao Jiasi,Yang Huqing,Chen Huiyun
The effects of lauroyl arginate ethyl (LAE), chitosan (CTS), and CTS-containing montmorillonite and LAE coatings (CML) on the quality maintenance and shelf life of minimally processed grapes stored at 4 °C for 20 days were investigated. Compared with the control, LAE, and pure CTS coatings, the CML coatings effectively decreased grape weight loss, respiration, and decay incidence as well as microorganism counts of total aerobic bacteria, yeasts, molds, and lactic acid bacteria in grapes. The CML coatings also showed better effects on delaying the tissue softening and browning due to the decreased activities of polygalacturonase, pectin methyl esterase, and polyphenol oxidase. CML coatings did not significantly change the content of soluble solids and the titratable acidity or the pH value of grapes, compared with other samples. However, CML coatings ensured higher content of ascorbic acid and sensory quality in grapes. The results confirmed that CML coatings improve microbiological safety and preserve nutritional and sensory quality of minimally processed grapes.
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Journal of Biomedical Nanotechnology
·
2014
·Vol. 10
·期 6
·948-958
·
IF 7.578
Liu Yongjun,Liu Chunxi,Li Min,Liu Fengxi,Feng Lixia,Zhang Li,Zhang Na
Multifunctional nanoparticles assembled from multi-block polymers are now one of the most convenient and convincing carriers for target drug delivery. Multi-block polymers could provide multi-functions such as sufficient drug loading capability and efficient target ligand coupling potency.
In this article, novel multi-block polymer poly(lactic acid)–poly(ethylene glycol)–poly(L-lysine) (PLA-PEG-PLL) with relatively precise block molecular weight were synthesized by new method which we called polymer–polymer conjugation. This method conjugated different polymer
blocks by reactions between the terminal active groups of different blocks, thus simplified the synthesis procedure. The obtained PLA-PEG-PLL was characterized by 1H NMR and gel permeation chromatography. The controlled drug delivery capability and the target ligand coupling potency
of PLA-PEG-PLL were verified using paclitaxel (PTX) as model drug and vascular endothelial growth factor (VEGF) antibody as target ligand. The PTX-loaded PLA-PEG-PLL nanoparticles (PNP) and VEGF antibody modified PTX-loaded PLA-PEG-PLL nanoparticles (VPNP) were prepared using solvent diffusion
methods. The two nanoparticles showed spherical or ellipsoidal shapes with uniform particle size distribution (190.1 ± 1.27 nm and 203.6 ± 4.10 nm for PNP and VPNP, respectively) and positive zeta potential (23.76 ± 0.72 mv and 20.76 ± 0.34 mv for PNP and VPNP,
respectively). The cellular cytotoxicity, cellular uptake, in vivo therapeutic effects of the two nanoparticles were investigated. Cytotoxicity of VPNP against HepG2 cells was superior to that of PNP and Taxol®. The VPNP and PNP showed better antitumor efficacy in a murine model
bearing H22 compared with Taxol® and VPNP was the best. The study on cellular uptake indicated that the better antitumor efficacy of VPNP was attributed to the increased uptake of drug by tumor cells. These results demonstrated that PLA-PEG-PLL was a favorable multifunctional
material for drug target delivery and polymer–polymer conjugation was a promising method to fabricate novel multi-block polymers.
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