MACROMOLECULAR RESEARCH
·
2018
·Vol. 26
·期 3
·270-277
·
IF 1.767
Ruan Zheng,Yuan Pan,Jing Titao,Xing Tao,Yan Lifeng
A novel pH-sensitive carborane and cyanine dye conjugated polypeptide and its micelles has been designed and synthesized for potential near infrared fluorescence imaging-guided boron neutron capture therapy (BNCT). The amphiphilic polymer we synthesized with tertiary amine as functional group could self-assemble to micelle nanoparticles at neutral pH, and responsive to acidic solution for the disassembly of the micelles for drug release. The tertiary amine sensitive induced process was observed via dynamic light scattering (DLS) and transmission electron microscopy (TEM). The efficiency of cell killing ability is studied by MTT assays and fluorescence microscope measurement, indicating a new intelligent system for potential NIR imaging-guided therapy.
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高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2017
·Vol. 53
·期 4
·2351-2361
·
IF 2.599
Song Mei-Ru,Li Dong-Yao,Nian Fu-Yu,Xue Jin-Ping,Chen Juan-Juan
To improve the aqueous solubility and cancer targeting of the photosensitizers in photodynamic therapy (PDT), we encapsulated the photosensitizer in a biocompatibility and pH-sensitive drug delivery system, zeolitic imidazolate frameworks-8 (ZIF-8) nanospheres. Powder X-ray diffraction and electron microscopy show that our nanospheres are uniform and single-crystalline particles. Owing to the cleavage of zinc–ligand coordination bonds, more ZnPc–COOH were released much faster in the mild acidic conditions (pH 5.0 and 6.0) in comparison with physiological environment (pH 7.4). By incorporating ZnPc–COOH in ZIF-8, our nanospheres exhibited high singlet oxygen quantum yield and intracellular ROS generation. Cell viability experiments toward HepG2 cells demonstrated the low toxicity of ZIF-8 and the good anticancer efficacy of the nanospheres with low IC 50 values (4.2–4.9 μg/mL) under light illumination (670 nm, 1.5 J/cm 2 ). Collectively, these results suggested that our nanospheres are the promising pH-responsive drug delivery systems for PDT.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2022
·Vol. 29
·期 19
·27677-27687
·
IF 5.19
Long Yujiao,Li Hongna,Jin Hongmei,Ni Jinren
The chemical and biological mechanisms of electrochemical elimination of Microcystis aeruginosa ( M. aeruginosa ) using boron-doped diamond (BDD) anode were comparatively explored in three different electrolytes (chloride, sulfate, and phosphate solutions). The most efficient elimination of M. aeruginosa was observed in chloride solution, which was attributed to the greatest total long-lived oxidants from the favorable formation of active chlorine. Moreover, the high permeability of active chlorine resulted in profound intracellular damages to chlorophyll- a , microcystin-LR (MC-LR), superoxide dismutase (SOD) enzyme, and DNA in the chloride system. The change of membrane permeability and degradation of the released MC-LR induced by active chlorine were further confirmed by the increase of extracellular MC-LR in the initial 5 min and a complete decay in the subsequent 15 min, while the change in morphology of algae cells was insignificant from SEM images. In sulfate and phosphate electrolytes, membrane damages were much more pronounced based on lipid peroxidation observation, although changes in cell morphology was found more significant in phosphate system. The higher concentrations of oxidants (·OH, O 3 , H 2 O 2 , S 2 O 8 2− ) generated in sulfate than in phosphate solution explained the greater efficiency of electrochemical elimination of M. aeruginosa in the sulfate electrolyte in terms of changes of cell density, OD 680 , chlorophyll- a , MC-LR, lipids, SOD enzyme, and DNA.
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高影响因子
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
·
2017
·Vol. 99
·期 4
·438-444
·
IF 1.412
Wang Se,Wang Zhuang,Chen Mindong,Fang Hao,Wang Degao
Combined toxicity and oxidative stress biomarker responses were determined for tetrabromobisphenol A (TBBPA) and sulfadiazine (SDZ) to the unicellular green alga Scenedesmus obliquus . Concentration–response analyses were performed for single toxicants and for mixtures containing TBBPA and SDZ with two different mixture ratios. The effect concentrations and the observed effects of the mixtures were compared to the predictions of the joint toxicity by the concentration addition (CA) model and independent action (IA) model. Results showed that the observed joint toxicity was within the scope of the highest (TBBPA) and lowest (SDZ) toxicity observed for the individual components. Furthermore, co-exposure of S. obliquus to TBBPA and SDZ provided preliminary evidence that the mixtures induced oxidative stress leading to cell damage. The CA and IA models proved to be valid for the prediction of the joint toxicity of TBBPA and SDZ. This study highlights a combined environmental risk assessment for two emerging pollutants.
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高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2019
·Vol. 411
·期 25
·6645-6653
·
IF 3.286
Ma Xin,Lin Shijun,Dang Yunfei,Dai Yu,Zhang Xiaojin,Xia Fan
Carbon dot (CD)-based fluorescent probes have been widely exploited; however, multi-component detection using CDs without tedious surface modification is always a challenging task. Here, we develop a convenient and simple CD-based “on-off-on” fluorescent probe for detection of copper(II) ion (Cu 2+ ), ascorbic acid (AA), and acid phosphatase (ACP). Cu 2+ leads to the fluorescence quenching of CDs. The limit of detection (LOD) for Cu 2+ is 2.4 μM. When AA is added into the CDs + Cu 2+ solution, Cu 2+ is reduced by AA to Cu + , causing the fluorescence recovery of CDs. The fluorescent intensity linearly correlates with the concentration of AA in the range of 100–2800 μM with LOD of 60 μM. Besides, the probe has potential application for detection of AA in real samples such as VC tablets, orange juice, and fresh orange. The probe can also indirectly detect ACP that enzymatically hydrolyzes ascorbic acid-phosphate (AAP) to produce AA. This work expands the application of CDs in the multi-component detection and provides a facile fluorescent probe for detection of AA in real samples.
Graphical abstract
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高影响因子
MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 5
·1305-1313
·
IF 4.58
Zhou Lili,Wang Jian,Chen Zhongping,Li Junlong,Wang Ting,Zhang Zhang,Xie Guoming
MicroRNAs (miRNAs) play a considerable role in cancer occurrence and development, and have been identified as promising noninvasive biomarkers. The authors describe a voltammetric method for the determination of the cancer biomarker microRNA-21 (miRNA). It is based on a combination of a universal DNA signal transducer and isothermal target recycling amplification. A hairpin capture probe is bound to the target miRNA to form a duplex structure and to create a toehold in the transducer for initiating the target recycling amplification reaction. In contrast to traditional capture probes, a mismatched site is introduced to improve its ability to capture the target. In order to reduce the complex design procedures of the sequence and widen the applicability of this method, a signal transducer is introduced. Under optimal conditions, response to target miRNA is linear in the 0.5 to 2000 pM concentration range, with a 56 fM. detection limit (at an S/N ratio of 3). In order to characterize the process of target recycling and the stepwise modification of the electrode, real-time fluorescence, agarose gel electrophoresis, cyclic voltammetry, electrochemical impedance spectroscopy and chronocoulometry were used. The results indicate that this isothermal target recycling amplification results in an electrochemical biosensing scheme with wide potential for sensing other bioanalytes.
Schematic illustration of the electrochemical biosensing platform for miRNA-21 detection based on isothermal target recycling amplification and DNA signal transducer triggered strategy.
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Nature Communications
·
2021
·Vol. 12
·期 1
·1-9
·
IF 14.919
Yang Yonggang,Wang Zegao,Gan Cuifen,Klausen Lasse Hyldgaard,Bonné Robin,Kong Guannan,Luo Dizhou,Meert Mathijs,Zhu Chunjie,Sun Guoping,Guo Jun,Ma Yuxin,Bjerg Jesper Tataru,Manca Jean,Xu Meiying,Nielsen Lars Peter,Dong Mingdong
Long-distance extracellular electron transfer has been observed in Gram-negative bacteria and plays roles in both natural and engineering processes. The electron transfer can be mediated by conductive protein appendages (in short unicellular bacteria such as Geobacter species) or by conductive cell envelopes (in filamentous multicellular cable bacteria). Here we show that Lysinibacillus varians GY32, a filamentous unicellular Gram-positive bacterium, is capable of bidirectional extracellular electron transfer. In microbial fuel cells, L. varians can form centimetre-range conductive cellular networks and, when grown on graphite electrodes, the cells can reach a remarkable length of 1.08 mm. Atomic force microscopy and microelectrode analyses suggest that the conductivity is linked to pili-like protein appendages. Our results show that long-distance electron transfer is not limited to Gram-negative bacteria.
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高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 8
·1-10
·
IF 5.479
Li Xiaohua,Wang Shanshan,Meng Yuchan,Wang Xiao,Zhang Yue,Hun Xu
Copper(II) polyphthalocyanine (CuPPc) was combined with graphitic carbon nitride (g-C 3 N 4 ) to form a heterojunction with enhanced photoelectrochemical (PEC) signal. A sensitive PEC method was developed for determination of ractopamine based on a PEC inner filter effect between gold nanoparticles (AuNPs) and the g-C 3 N 4 /CuPPc. A gold electrode was modified with g-C 3 N 4 /CuPPc and the DNA was linked to the AuNPs. Initially, the PEC signal is weak due to the inner filter effect between the AuNPs and g-C 3 N 4 /CuPPc. In the presence of ractopamine, it interacts with the aptamer and the complementary chain (C chain) is released. This triggers the entropy-driven cyclic amplification and results in the release of the substrate B chain (SB chain) from three-dimensional DNA stabilizer. The probe is released from the electrode due to the interaction of probe DNA and the SB chain. As a result, the PEC signal increases linearly in the 0.1 pmol·L −1 to 1000 pmol·L −1 ractopamine concentration range. The detection limit is 0.03 pM, and the relative standard deviation is 3.4% (at a 10 pmol·L −1 level; for n = 11). The method has been successfully applied to the determination of ractopamine in pork samples.
Schematic presentation of detection method based on PEC inner filter effect between AuNPs and the g-C 3 N 4 /CuPPc being fabricated for ractopamine. 3D DNA was used as stabilizer to decrease the PEC blank signal.
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高影响因子
MICROCHIMICA ACTA
·
2014
·Vol. 182
·期 5
·1123-1129
·
IF 3.719
Wang Wenting,Wang Wei,Davis Jason J.,Luo Xiliang
We describe an aptasensor for the determination of dopamine in human serum and with ultrahigh sensitivity and selectivity. The sensor is based on a nanocomposite consisting of reduced graphene oxide (rGO) and the conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT). The PEDOT/rGO interface was prepared by electrochemical polymerization of EDOT using graphene oxide as the dopant which is later electrochemically reduced to form rGO. Subsequent covalent modification of the high surface area composite with a selective aptamer enables highly sensitive and selective detection by differential pulse voltammetry. The calibration plot established at a working voltage of 160 mV displays a linear response in the 1 pM to 160 nM concentration range and an unprecedented detection limit of 78 fM. The sensor is fairly selective in not responding to common interferents, and is reusable after regeneration with a 7 M solution of urea. It was successfully applied to (spiked) serum samples and gave recoveries ranging from 98.3 to 100.7 %.
A highly sensitive and selective biosensor for dopamine was developed based on an aptamer-modifed conducting polymer (PEDOT) doped with reduced graphene oxide.
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高影响因子
MACROMOLECULAR RESEARCH
·
2022
·Vol. 30
·期 4
·238-244
·
IF 2.127
Twizeyimana Etienne,Zhang Shuang,Mukerabigwi Jean Felix,Ge Zhishen
Advances in designing smart hydrogels with the structures and properties similar to the interior of extracellular matrix have aroused the interest of researchers as promising cell support scaffolds in the field of tissue engineering. In this study, a novel hydrogel system is developed from natural polysaccharides that are sodium alginate, gelatin and carboxymethyl chitosan through Schiff base cross-linking mechanism. By mixing these three components, the hydrogel system with tunable strength and gelation time was obtained and abbreviated as oxACG hydrogel. Moreover, the obtained hydrogels are biocompatible and can sustain the growth and proliferation of Hela cells after 24 h incubation. The experimental results show that the hydrogel possesses a relatively short gelation time that is around 3 min, high gel strength and negligible cell cytotoxicity. The oxACG hydrogel composite exhibited desirable properties, and they can be used as high-performance multifunctional injectable hydrogels in cell therapy.
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高影响因子
MOLECULAR NEUROBIOLOGY
·
2026
·Vol. 63
·期 1
·956
·
IF 5.5
Cao Yuting,Tang Peipei,Feng Dan,He Hankuan,Wu Han,Liu Yang,Tian Jinfeng,Hu Kongzhen,Zhong Yuhua
Fibrotic scar formation after ischemic stroke limits neuroplasticity and functional recovery. While estradiol (E2) exerts anti-fibrotic effects, the mechanisms by which high-frequency repetitive transcranial magnetic stimulation (rTMS) promotes post-stroke recovery remain poorly understood. We investigated whether rTMS attenuates post-ischemic brain fibrosis via aromatase-dependent local E2 synthesis. Male Sprague–Dawley rats subjected to permanent middle cerebral artery occlusion received high-frequency rTMS starting post-stroke day 3. Fibrosis and metabolism were evaluated using longitudinal dual-tracer [ 18 F]AlF-FAPI-42 and [ 18 F]FDG PET/CT on days 3 and 14, alongside histological and biochemical assessments. The aromatase inhibitor letrozole was used to probe mechanistic dependence. Additionally, we retrospectively analyzed dual-tracer PET/CT data from 10 ischemic stroke patients. In rats, rTMS significantly reduced [ 18 F]AlF-FAPI-42 uptake, decreased collagen deposition and fibrotic marker expression, increased [ 18 F]FDG uptake, and improved neurological outcomes. rTMS also upregulated aromatase expression and elevated peri-infarct E2 levels; these anti-fibrotic and metabolic benefits were no longer observed under letrozole treatment. Brain E2 levels correlated negatively with [ 18 F]AlF-FAPI-42 uptake and positively with motor performance. Corroborating the preclinical model, clinical imaging revealed elevated [ 18 F]AlF-FAPI-42 uptake in the ischemic hemisphere of patients, which was inversely associated with [ 18 F]FDG uptake. These findings demonstrate that high-frequency rTMS attenuates post-stroke fibrosis and promotes metabolic recovery partly through aromatase-dependent elevation of local E2. Furthermore, dual-tracer [ 18 F]AlF-FAPI-42/[ 18 F]FDG PET provides a novel, non-invasive method for monitoring fibrosis–metabolism coupling. The clinical findings highlight the translational relevance of this relationship, suggesting that tracking fibrotic remodeling could guide post-stroke rehabilitation.
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高影响因子
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
·
2026
·
IF 3.4
Li Ya,Lu Mingfei,Pan Yuhua,Liang Jianfeng
In order to investigate characteristic marker metabolites and their grade-dependent variations in Liupao tea, non-volatile compounds from four grades of Liupao tea were analysed by combining sensory evaluation with UPLC-Q-TOF-MS based untargeted metabolomics. Sensory evaluation revealed that decreasing Liupao tea grade was accompanied by reduced stale flavour, gradual increases in bitterness and astringency, and an overall deterioration of sensory quality. In total, 4 099 non-volatile compounds were detected, of which 181 metabolites were common across all four grades. 14 significantly differential metabolites, including EC, EGC and L-theanine, displayed regular grade-related changes with Pearson correlation coefficients of |r| ≥ 0.7. Multivariate statistical analysis confirmed strong correlations between these 14 key compounds and both tea grade and sensory attributes. A PLS regression model built upon the 14 markers achieved an internal fitting R²of 0.9929. It should be noted that this model was trained solely on samples originating from a single tea garden, multi-sample external validation is required to verify its generalisability. This study provides theoretical references for unravelling flavour differences among differently graded Liupao tea.
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