ANALYTICA CHIMICA ACTA
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2026
·
IF 6.1
Nanke Ma,Anqi Wei,Huiling Peng,Lixia Xie,Yuan Jin,Zhengzhi Yin,Guangli Li
Background Chirality plays a critical role in drug efficacy and environmental safety. The antibacterial activity of ofloxacin resides almost exclusively in its S-enantiomer (levofloxacin, LEV), while the R-enantiomer contributes little to therapeutic effects but may pose unnecessary risks. Rapid and reliable methods for enantioselective determination of ofloxacin enantiomers are therefore essential for pharmaceutical quality control, food safety monitoring, and environmental analysis. However, existing electrochemical sensors often suffer from limited enantioselectivity and insufficient sensitivity. To address this challenge, a novel enantioselective electrochemical platform is developed. Results A β-cyclodextrin/cerium-doped copper metal-organic framework/carboxylated multi-walled carbon nanotube composite (β-CD/Ce-doped Cu-MOF/MWCNTs-COOH) was fabricated on a glassy carbon electrode. The sensor achieves discrimination of ofloxacin enantiomers with an I L /I D ratio of 3.39. DFT calculations at pH 4.0 reveal that β-CD binds LEV with higher adsorption energy (−63.8 kJ/mol) than DEX (−58.3 kJ/mol), confirming the superior response for LEV originates from stereospecific inclusion. Quantitative analysis of LEV shows two linear ranges (0.03–1 μM and 1–10 μM) with a low detection limit of 0.01 μM. The sensor also enables accurate determination of LEV percentage in enantiomer mixtures across 0–100%. Excellent selectivity against common interferents, good reproducibility (RSD = 0.98%), repeatability (RSD = 1.26%), and long-term stability (95.2% retention after 21 days) are demonstrated. Practical applicability is validated by detecting LEV in commercial eye drops, honey, and tap water with satisfactory recoveries (96.0–108.0%). Significance and novelty This work presents the first application of Ce-doped Cu-MOF/β-CD/MWCNTs-COOH composites for the enantioselective electrochemical sensing of ofloxacin, a chiral fluoroquinolone antibiotic. The synergistic combination of high conductivity, abundant catalytic sites, and stereospecific host-guest recognition provides a generalizable strategy for high-performance enantioselective sensing, with promising applications in pharmaceutical, food, and environmental analysis.
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ACS Sustainable Chemistry & Engineering
·
2026
·
IF 7.6
Huabiao Miao,Junlong Wu,Menghan Yang,Yuqi Lu,Chunjiao Xie,Sha Wen,Bo Xu,Zunxi Huang,Chengbo Zhang
Human milk oligosaccharides (HMOs) and chitin oligosaccharides possess unique nutritional and prebiotic properties, driving interest in their sustainable synthesis. The key enzyme for their production is β-N-acetylhexosaminidase. Herein, we characterized Nag1420 (accession: PQ041313), a novel GH3 family enzyme from Cellulosilyticum sp., which exhibits both hydrolytic and transglycosylating activities. Substrate specificity analysis revealed a higher activity toward the natural substrate chitobiose than the synthetic analog p-nitrophenyl N-acetyl-β-d-glucosaminide (pNP-GlcNAc), enabling complete hydrolysis to N-acetylglucosamine. Notably, Nag1420 synthesized Lacto-N-triose II (LNT II) via transglycosylation when β-lactose was present as an acceptor. Crucially, in a cascade reaction with Chitinase, Nag1420 directly converted chitin waste into the high-value LNT II. This study provides insights into the green and low-cost biosynthesis of high-value-added carbohydrates.
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Analytical Methods
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2026
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IF 3
Yao Tian,Mengsi Liang,Yongdong Wei,Yuxin Wang,Ning-Bo Li,Yaoyong Wang
Zearalenone (ZEN) is a carcinogenic mycotoxin commonly found in grains, cereals, and dairy products, posing cancer risks to humans and animals. Chronic high-dose ZEN exposure can cause liver disease, immunosuppression, kidney damage, and tumorigenesis. Sensitive and efficient ZEN detection is therefore essential for protecting human and animal health. To meet the need for sensitive, selective, and low-cost mycotoxin sensors in real food samples, we developed a novel ratiometric electrochemical aptamer sensor for ZEN detection. The sensor design utilizes β-cyclodextrin-functionalized graphene (CG) to effectively enhance electron transfer efficiency, combined with host–guest recognition between ferrocene (Fc) and β-cyclodextrin (β-CD), as well as Au-S covalent bonding. The 5′-SH and 3′-MB-modified dual-labeled ZEN aptamer (MB-Apt-SH) was immobilized via β-CD/Fc inclusion, with signal amplified by AuNPs binding to -SH. Without ZEN, the aptamer stays on the electrode, giving high IMB and low IFc. With ZEN, it binds the target, pulling it away. Adding Fc-COOH fills the β-CD cavities, increasing IFc and decreasing IMB, thus raising the IFc/IMB ratio proportionally to ZEN concentration. The sensor exhibits a wide linear range from 1 pg/mL to 1000 ng/mL, with a detection limit of 0.4344 pg/mL. Furthermore, the method was successfully applied to the determination of ZEN in wine samples, yielding recovery rates between 94.00% and 104.60%, demonstrating excellent accuracy and applicability. These results indicate that the MCH/AuNPs/SH-Apt-MB/CG/GCE platform is a highly sensitive and selective electrochemical sensor for ZEN detection.
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Nanomaterials
·
2026
·Vol. 16
·期 6
·378
·
IF 4.8
Hao Li,Lin Zhang,You Long,Chao Shen,Song Zhang,Fang Chen,Nan Chen,Chenghong Huang
Paclitaxel is a first-line anticancer drug, but its low water solubility impedes bioavailability. The purpose of this study is to estalish a delivery strategy via carboxymethyl chitosan (CMCS)-encapsulated 6-deoxy-6-mercapto-β-cyclodextrins (dmβCDs)-modified concave cubic gold (CCGs) to achieve PTX release. CCGs were initially synthesized by the one-pot method and further modified by dmβCDs, the dmβCDs can effectively capture PTX molecules, followed by encapsulation with CMCS, and then prepare pH-responsive CMCS/dmβCDs/CCGs nanocarriers after lyophilization. Results indicated that desirable hexagonal CCGs with 50 ± 5 nm size can be obtained by adjusting H 2 O 2 and HClO concentration. FT-IR, Raman and XRD spectra had confirmed dmβCDs successfully grafted to the surface of CCGs. Drug loading experiments demonstrated that the nanocarrier encapsulated PTX in amorphous powder or molecular form have a capacity of 55.05 µg/mL. Drug release experiments revealed PTX release from CMCS/dmβCDs/CCGs nanocarriers carrying a typical pH-responsive profile and indicating earlier release in an acidic environment than in a neutral or alkaline environment. The proposed method can be utilized to effectually achieve high-efficiency solubilization and targeted release inside tumor cells of PTX.
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ANALYTICA CHIMICA ACTA
·
2026
·
IF 6.1
Wenli Lai,Qian Lin,Cunshan Zhang,Bin Chen,Jinyu Li,Lingting Wu,Maoru Lin,Hengfen Dai,Liqing Lin
A novel oxygen vacancy-rich PRT@MnO 2-x nanozyme was fabricated via a facile one-pot ultrasonic method. • The as-prepared material possesses prominent oxidase-like activity, relying on in-situ water activation rather than exogenous oxygen. • A sensitive colorimetric/fluorescent dual-mode sensing platform was constructed for β-Gal activity with low detection limits. • The developed strategy shows satisfactory performance in human serum, possessing promising clinical application potential. Vacancy engineering has been widely applied to modulate the enzyme-like activity of nanomaterials by adjusting their surface/electronic structures and creating additional active sites. Regulating oxygen vacancies in nanomaterials and elucidating their oxygen activation mechanisms are crucial for designing high-performance nanozymes. Herein, we employed KMnO 4 as the manganese source and protamine as the stabilizer to prepare transition metal manganese oxide (PRT@MnO 2-x ) rich in oxygen vacancies. The as-prepared PRT@MnO 2-x exhibited superior oxidase-like activity, which is proposed to originate from oxygen vacancy-mediated electron transfer and valence band hole-driven water oxidation to ·OH. Steady state kinetic constant (K m ) suggested that PRT@MnO 2-x had a stronger affinity toward 3,3′,5,5′-tetramethylbenzidine and possessed prominent catalytic performance (K m and V max were 0.074 mM and 0.031 mM s −1 mg −1 ). An innovative method is proposed to employ PRT@MnO 2-x nanosheets with abundant oxygen vacancies for colorimetric/fluorescent dual-mode detection of β-Galactosidase (β-Gal) activity. Notably, the OXD-like activity of PRT@MnO 2-x is largely independent of dissolved oxygen or molecular oxygen. Instead, it facilitates electron transition from the valence band to the conduction band through the form of oxygen vacancies, enabling the oxidation of water to ·OH on the valence band of PRT@MnO 2-x . The detection limits (LOD) determined by colorimetric and fluorescence analysis for β-Gal activity are 0.015 U/L and 0.054 U/L respectively, and satisfactory spiked recovery results were achieved in human serum samples. This method has the advantages of low cost, simple operation and rapid response. The dual-mode signal sensor is expected to provide more accurate and robust detection of β-Gal than the single-mode probe, and has a wider application prospect. Download: Download high-res image (203KB) Download: Download full-size image
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CHEMICAL ENGINEERING JOURNAL
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2026
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IF 12.5
Xinran Yin,Dan Zhu,Xinyan Zhang,Ruiwen Cheng,Haonan Ren,Yanan Sun,Demei Meng,Weina Li,Fansheng Cheng
Electrospun CS/PVA films with THY/TBZ@HPβCD ICs enabled humidity-gated preservation. • Synchronized dual-release of THY/TBZ at 93% RH via non-Fickian transport. • Films exhibited 94.2% ABTS scavenging and synergistic antibacterial efficacy. • Shiitake browning index dropped from 74.4% to 43.2% with minimal weight loss. • Humidity-triggered release suppresses PPO melanization. The intense postharvest respiration of edible fungi rapidly establishes a near-saturated humidity microenvironment that accelerates microbial spoilage and PPO-associated browning. To harness this detrimental moisture as an intrinsic trigger, we developed a humidity-gated electrospun nanofibrous film by co-loading two individually prepared inclusion complexes (THY@HPβCD and TBZ@HPβCD) into a chitosan/ poly(vinyl alcohol) (CS/PVA) matrix (TTIC-CP). The film exhibited enhanced wettability and robust physicochemical properties, enabling relative humidity (RH)-triggered synchronized co-release of both actives. Under 93% RH, cumulative release reached 88.07% (THY) and 87.30% (TBZ), and kinetic fitting indicated non-Fickian transport governed by coupled diffusion and matrix relaxation/erosion. Checkerboard assays further confirmed THY-TBZ synergy against E. coli , S. aureus and P. tolaasii with minimum FICI values of 0.50, 0.38 and 0.50. Accordingly, 8TTIC-CP delivered strong antioxidant activity (94.17% ABTS scavenging efficiency at 650 s) and effective antibacterial protection (75.63% inhibition against P. tolaasii ). In shiitake packaging, 8TTIC-CP markedly delayed browning and quality loss, reducing the browning index from 74.37% (control) to 43.17% and lowering weight loss to 4.77%, while alleviating oxidative and enzymatic browning responses. Comparable preservation efficacy was also confirmed in Oudemansiella raphanipes and Stropharia rugosoannulata . This work provides a practical humidity-gated dual-active packaging strategy for high-respiration edible fungi and related fresh produce. Download: Download high-res image (129KB) Download: Download full-size image
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LWT-FOOD SCIENCE AND TECHNOLOGY
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2026
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IF 7.1
Shuai Zhao,Ye Xue,Ruiyao Chen,Xiangyi Ge,Ning An,Yawei Shi
The walnut green husk is a by-product generated during walnut processing, the waste of walnut green husk posed a potential threat to agricultural ecosystem. We aimed to developed a biodegradation technique to promote the lignocellulose degradation of walnut green husk. The results suggested that the activity of cellulose-degrading enzyme secreted by Klebsiella variicola M6 reached the peak on the 3rd day of degradation. Moreover, the degradation rate treated with K. variicola M6 was significantly higher ( p < 0.05), and FTIR and SEM also characterized the structure alterations of walnut green husk. Additionally, the β-xylosidase (KvXyl43) from K. variicola M6 was expressed, showing optimal activity at 35°C and pH 6.0. Combining K. variicola M6 (4% inoculation) with KvXyl43 (5 U/g supplementation), the degradation rate of walnut green husk reached 78.6±2.9% after 10 days, demonstrating the efficacy of strain-enzyme synergy in lignocellulose degradation. This research was pivotal for the degradation of walnut by-products.
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POLYMER
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2026
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IF 5.1
Yao Long,Jiahao Zhou,Xupeng Han,Min Zhang,Yayan Wang,Jiaping Tan,Meilin Liu,Lijun Li,Kun Liu
To develop myrcene-based polymers with both functionalization characteristics and controllable structure, this paper employs 1-(vinylbenzyl) piperidine (VBMP) as a tertiary amine functional monomer and achieves the controlled copolymerization of VBMP with β -myrcene (MY) under mild conditions above room temperature in cyclohexane using living anionic polymerization. By leveraging the dual role of VBMP as both a comonomer and a polarity regulator, and optimizing the monomer feed ratio, poly(MY- co -VBMP) copolymers with controllable molecular weight and narrow molecular weight distribution (1.11 < PDI < 1.23) are successfully prepared. 1 H NMR and FTIR characterizations confirm that VBMP is uniformly inserted into the polymer backbone via in-situ copolymerization, achieving a quantitative functionalization. The reactivity ratios are determined using the Fineman-Ross and Kelen-Tüdős methods, yielding r MY = 4.62-4.80 and r VBMP = 0.43-0.54. Moreover, DSC analysis shows that with increasing VBMP content, the glass transition temperature ( T g ) of the copolymers gradually rises from −18 °C to 25 °C, accompanied by significantly enhanced chain segment regularity. In addition, the LAP chain-end active center coordination mechanism and the N-Li coordination synergy mechanism within the polymer chain are proposed to rationalize the experimental results. Overall, this polymerization system not only exploits the weak regulatory effect of the tertiary amine group of VBMP on anionic polymerization but also achieves quantitative functionalization of β -myrcene copolymers, providing an efficient route to functional materials possessing both elastic properties and reactive sites.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2026
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IF 6.7
Wenjing Fan,Changgeng Li,Runze Zhao,Tang’en Shi,Liangwen Li,Zhichao Chen,Zichen Yu,Ling Ma,Siyu Zhao,Qingyang Xu
The demand for β-thymidine primarily stems from its role as a raw material and intermediate in antiviral drug production. To address this, β-thymidine was synthesized de novo using Escherichia coli. We engineered a high-yielding β-thymidine strain based on the orotate high-yielding strain Ora14–4. First, we introduced the pyrFE gene cluster from Bacillus subtilis to direct the metabolic flux toward β-thymidine synthesis. Genes involved in β-thymidine catabolism and precursor branching were knocked out, and key enzymes in the β-thymidine biosynthetic pathway were optimized. Enhancing the folate cycle and glycine cleavage system provided sufficient one-carbon donors for deoxythymidine monophosphate synthesis. The Entner-Doudoroff pathway from Zymomonas mobiliz was introduced to enable NADPH production. Last, a growth-adaptive promoter regulated tmk gene expression to minimize carbon loss. The final β-thymidine titer was 25.4 g/L, with a conversion rate of 0.079 g/g glucose, which is, to the best of our knowledge, the highest titer reported to date for de novo microbial production of β-thymidine.
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BIOMASS & BIOENERGY
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2026
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IF 7
Jing Bai,Mengqi Wang,Yang Xiao,Xiangyu Cheng,Weiyu Chen,Haoran Wu,Chun Chang
A single-step removal of γ-carbinol from lignin β-O-4 linkages was reported. • Key parameters for γ-carbinol removal were systematically screened and optimized. • Ru/C system enabled efficient upgrading of monophenols from birch-derived bio-oil. • The selectivity and yield of 2,6-dimethoxy-4-ethylphenol reach 53.02% and 39.33%. • A promising route for lignin valorization was proposed. Development of efficient strategies for converting the C3 β-O-4 motif of lignin into a simplified C2 β-O-4 structure is of great significance, yet it remains a formidable challenge. Herein, we report the use of Ru/C as a catalyst to convert α-hydroxy-free C3 β-O-4 fragments into C2 β-O-4 fragments via a one-step strategy for the removal of the γ-carbinol group. A series of lignin-derived phenolic model compounds were selected to systematically investigate the effects of key reaction parameters, including temperature, reaction time, catalyst dosage, and substituent structure on the removal of the γ-carbinol group. On the basis of the obtained reaction rules and optimized parameters, the Ru/C-catalyzed system was successfully applied to the upgrading of monophenolic components in bio-oil obtained from the depolymerization of authentic birch lignin. Under optimized reaction conditions, 4-(3-hydroxypropyl)-2,6-dimethoxyphenol in the bio-oil achieved a conversion of 74.18%, affording the target product 2,6-dimethoxy-4-ethylphenol with a selectivity of 53.02% and a yield of 39.33%. This study provides a novel and efficient approach for the valorization of lignin-derived bio-oil, laying a solid foundation for the sustainable utilization of lignin resources. Download: Download high-res image (376KB) Download: Download full-size image
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ENZYME AND MICROBIAL TECHNOLOGY
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2026
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IF 3.9
Mingrong Cui,Yaru Huang,Yanchun Tao,Ying Zhang,Xiuhua Zhao,Xiaoqiang Chen
Senescence-associated β-galactosidase (SA-β-Gla) is a key enzymatic biomarker of cellular senescence and aging, making its inhibition a promising strategy for developing anti-aging functional foods. Sea buckthorn ( Hippophae rhamnoides L . ) berries are rich in flavonoids with antioxidant and anti-aging potential; however, conventional extraction methods prioritize yield over the retention of bioactivity. In this study, we developed a bioactivity-oriented (BO) extraction strategy guided by β-Gla inhibitory activity, using ultrasound-assisted enzymatic extraction optimized by response surface methodology. Under the optimal BO conditions, the β-Gla inhibition rate reached 74.71 ± 1.88%. After purification, the BO extract contained significantly higher levels of the major flavonoids rutin, taxifolin, and isorhamnetin-3-O-rutinoside (collectively accounting for 54.0%) compared to the yield-oriented (YO) extract (27.5%). Consequently, the purified BO extract exhibited stronger antioxidant activity and β-Gla inhibition (IC₅₀ = 30.33 μg/mL) than the YO extract (IC₅₀ = 47.62 μg/mL). Correlation analysis indicated that the enhanced bioactivity resulted from synergistic interactions among multiple flavonoids. These findings demonstrate that the BO strategy selectively enriches high-activity flavonoid components and provides an effective processing approach to produce sea buckthorn-derived ingredients with targeted anti-aging properties for functional food applications.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2026
·
IF 15
Ma Tianyu,Li Qiang,Chen Xingwu,Dong Jixiang,Jiang Yuqing,Li Shanshan
Bioactive peptides are promising candidates for hair regeneration, but their topical application is often limited by poor skin delivery and the need for frequent dosing. To overcome these challenges, we designed a palmitoylated peptide, Pal-MPAPO, and incorporated it into a hyaluronic acid-based hydrogel microneedle (MN) system for localized transdermal delivery. The fabricated MNs showed adequate mechanical strength for skin insertion and provided a time-dependent, prolonged release of Pal-MPAPO. In human dermal papilla cells (DPCs), Pal-MPAPO activated the Wnt/β-catenin pathway and increased intracellular cyclic adenosine monophosphate (cAMP) levels in a predominantly VPAC1-dependent manner. Additional VPAC2 validation did not reveal a significant contribution to cAMP signaling under the tested conditions. Functional interactions between Pal-MPAPO and VPAC1 were supported by molecular docking, molecular dynamics simulations, receptor knockdown, and fluorescence imaging. In a C57BL/6 mouse model, Pal-MPAPO-loaded MNs significantly promoted hair regrowth, achieving efficacy comparable to minoxidil but at a reduced application frequency. These findings identify Pal-MPAPO as a potential peptide candidate for hair regeneration and suggest that hydrogel MN-assisted delivery provides a localized peptide administration strategy. Graphical The alternative text for this image may have been generated using AI.
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