MICROCHIMICA ACTA
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2025
·Vol. 192
·期 7
·1-12
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IF 5.3
Xu Zixuan,Zhang Yumin,Jiang Minqiang,Wang Yuheng,Li Min,Li Lijie,He Gaohong,Zhang Wenjun
Of tunable porosity, high specific surface area and excellent stability, the metal–organic frameworks (MOFs) as nanozyme capably not only catalyze the bio-substrate but load the natural enzyme, so as for the cascade catalyzation achievement. However, the limited mass transfer rate and natural enzyme abscission of MOFs are still fatal for the practical application. Herein, taking P-aminobenzoic acid (PABA) as a pore-size regulator and boronic acid (BA) as the bio-affinity sorbent, BA-MIL-100(Fe)-PABA of enlarged pores and better affinity was acquired as the peroxidase to ensure the efficient mass transfer and enzyme protection. Via the thorough investigation, the maximum reaction rate of H 2 O 2 catalyzation by BA-MIL-100(Fe)-PABA was up to 2.62 × 10 −7 M·s −1 with \({K}_{m}\) as low as 0.357 mM, along with the LOD of 0.77 μM. Loading GOx, a cascade platform of GOx@BA-MIL-100(Fe)-PABA was subsequently obtained to detect the blood glucose stable-selectively, with the wide linear response range (0.025–1.5 mM) and the LOD of 0.017 mM. The recycled catalytic activity of the platform was better 68% during 12 cycles. A new method for constructing defective MOFs at room temperature was thus proposed to achieve an efficient mass transfer and a better bio-affinity for glucose detection with high sensitivity and selectivity, paving the way for more sensitive detection and advanced applications. Graphical abstract
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2025
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IF 3.8
Yu Luyao,Liu Meichen,Niu Ziyi,Zhang Jiarui,Deng Yue,Zhou Xinyue,You Jiansong,Xue Hongyu,Yin Lei,Shi Meiyun
Monodisperse polyethylene glycol (PEG) derivatives offer significant advantages over conventional polydisperse PEGs for biomedical applications due to their precisely defined molecular structures. This study establishes an eco-efficient, selective, and sensitive analytical assay integrating microscale solid-phase extraction (μ-SPE) with ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) to investigate the cellular uptake of HO-PEG 8 -OH polymers in MCF-7 cells. The method achieved greater than 91% recovery from 20 μL lysates using M-PEG 6 -OH as the internal standard, with validated linearity (10–1000 ng/mL, R > 0.997), accuracy (relative error < ± 7.49%), and precision (RSD < 7.50%). The novelty of the assay is the harmonization of analytical performance with green analytical chemistry principles. The validated method provides a robust platform for studying monodisperse PEG derivatives while addressing growing demands for sustainable analytical technologies. Green analytical chemistry metric assessments confirmed the environmental sustainability of the method. Cellular pharmacokinetic analysis revealed time-/concentration-dependent uptake kinetics of HO-PEG 8 -OH polymers in MCF-7 cells, showing 3.2-fold accumulation between 0.5 and 48 h exposure. These findings offer important insights for PEG-based drug delivery system optimization and establish a new standard for environmentally conscious bioanalytical method development. Graphical
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Journal of Thermal Science
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2025
·
IF 2.6
Yu Hao,Wang Weiran,Bian Zhiguo,Ma Xiaotong,Lu Xiao,Chang Long,Zhang Wan
The modification with dark metallic oxide is identified as the crucial strategy to enhance optical absorptions of calcium-based materials for the direct solar-driven thermochemical energy storage. The effect of modification on the heat release behavior in carbonation of calcium-based material has been widely investigated, but its effect on the heat storage behavior in calcination is lacking of sufficient research, typically for low-cost calcium resource such as carbide slag. The Fe-modified and Mn-modified carbide slags for CaCO 3 /CaO heat storage were synthesized and their optimum decomposition temperatures, effective heat storage conversions, heat flows and heat storage rates in endothermic stage were investigated. Although the Fe modification exacerbates the CaO sintering due to the formation of Ca 2 Fe 2 O 5 , that is still effective in reducing the regeneration temperature of CaO in CaCO 3 /CaO cycles. The Mn modification enhances significantly sintering resistance by forming the CaMnO 3 and its transformation into Ca 2 MnO 4 . The effective heat storage conversion of Mn-modified carbide slag after 30 cycles is 3.2 times as high as that of untreated carbide slag. Mn-modified carbide slag exhibits the lowest regeneration temperature and the highest heat storage rate after cycles. The loose and stable porous structure of Mn-modified carbide slag contributes to its superior endothermic performance. Therefore, Mn-modified carbide slag seems to be the potential candidate for calcium looping thermochemical heat storage.
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CELLULOSE
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2025
·
IF 4.8
Yan Weimin,Hou Chengmin
Cationic copolymer carboxymethyl cellulose-graft-poly(methacryloxyethyl trimethylammonium chloride) (CMC- g -PDMC) was synthesized via free radical polymerization and further modified with glutaraldehyde (GA) to immobilize horseradish peroxidase (HRP). DMC, a cationic monomer, was grafted onto CMC to enhance electrostatic adsorption, while GA provided covalent crosslinking sites for HRP. The GA-crosslinked copolymer (CMC- g -PDMC-GA) enabled efficient enzyme immobilization on glass surfaces for batch and continuous catalysis. After 10 cycles of batch catalysis, the immobilized HRP retained 98% of its initial activity. In continuous flow (2 h), the activity remained stable, with a 1.6-fold higher efficiency on NaOH-treated glass compared to untreated surfaces. HRP alone or without GA crosslinking showed negligible immobilization.
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JOURNAL OF NANOBIOTECHNOLOGY
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2025
·Vol. 23
·期 1
·1-26
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IF 12.6
Jia Wenjing,Yue Huilan,Liu Liying,Wang Luya,Zhang Lin,Tao Jihong,Zhou Guoying,Wei Lixin,Dong Hongxin,Dhondrup Rinchen,Zhao Xiaohui
β-HgS as the main component of Tibetan medicine Zuotai (ZT) is widely used in the treatment of central nervous system diseases. Although the synergism of HgS has been clinically verified for more than 2000 years, its synergetic mechanism is still an unsolved mystery and a huge challenge. Notably, we clearly and intuitively demonstrate that Zuotai or β-HgS is auto-synthesized by organisms into spherical HgS nanoparticles with protein corona, with an overall particle size of 30–195 nm and a core of HgS NPs of 5–7 nm. Further research showed that HgS NPs facilitated the transport of Oxiracetam (ORT), Memantine Hydrochloride (MH) and Entinostat (MS-275) across the blood-brain barrier (BBB), especially the brain accumulation of MS-275 increased by more than three times. Meanwhile, HgS NPs enhanced the effect of MS-275 on spatial learning and memory ability of APP/PS1 mice. Further studies confirmed that HgS NPs increased the permeability of the blood-brain barrier (BBB) by regulating endocytosis (Upregulation of caveolin, clathrin, and dynamin, downregulation P-gp) thereby facilitate the transport of drugs across BBB with more than threefold higher brain accumulation and long-lasting efficiency enhancement (4 days). Our findings reveal that these in vivo-generated HgS NPs provide an efficient, convenient, and biocompatible drug delivery platform for crossing the BBB. Importantly, our findings offer new hopes and ideas for in-depth research on highly valuable and mysterious metal preparations in classic Chinese and Tibetan medicine. Graphical
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
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2025
·Vol. 36
·期 16
·1-19
·
IF 2.8
Li Minghua,Gu Xinyue,Chen Guohui,Chen Youmei,Zhu Qin,Yan Ya,Li Zhengui
In this study, CuInS 2 (CIS) was prepared using the liquid phase reflux method, and different amounts of activated carbon (AC) are loaded onto CIS via a wet grinding method to prepare AC/CIS composites with varying composite ratios. The crystal structure, surface elemental composition, oxidation states, morphology, specific surface area, and optical properties of CIS and AC/CIS were analyzed ussing techniques such as X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), high-resolution transmission electron microscopy (HRTEM), Brunauer–Emmett–Teller (BET) analysis, photoluminescence (PL), and ultraviolet–visible diffuse reflectance spectroscopy (UV–Vis-DRS). Rhodamine B (RhB) was used as a model pollutant to investigate the visible-light photocatalytic activity of CIS and AC/CIS. The optimal mass load ratio for AC was determined to be 3%. At this optimal ratio, AC/CIS demonstrated the capability to degrade 80% of RhB within a span of 2 h. The judicious incorporation of AC enhances the visible light absorption of CIS, accelerates electron migration, which facilitates the separation of photogenerated carriers, thereby increasing the visible-light photocatalytic activity and recyclability stability of CIS. Additionally, due to the electrophilicity of AC, the introduction of AC led to a transformation in the main active species during the photocatalytic process, changing from holes (h + ) and hydroxyl radicals (·OH) to superoxide radicals (·O 2 − ). These findings strongly suggest that AC/CIS holds great promise as a visible photocatalyst for effectively degrading pollutants in the environment.
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-13
·
IF 15.7
Qiu Yu,Sun Mingzi,Wu Jiandong,Chai Chunxiao,Wang Shengwei,Huang Hong,Zhao Xiao,Jiao Dongxu,Xu Shan,Wang Dewen,Ge Xin,Zhang Wei,Zheng Weitao,Song Yujiang,Fan Jinchang,Huang Bolong,Cui Xiaoqiang
The efficiency of the oxygen reduction reaction (ORR) is limited by the scaling relationship in the conventional oxygen associative pathway. To break such limitations, we present an approach to effectively activate the oxygen dissociative pathway through co-confining single p -block (In, Sn, Pb) atoms and interstitial H atoms within Pd metallenes, leading to good ORR performance. PdPbH x metallenes exhibit a high mass activity of 1.36 A mg −1 at 0.95 V (vs. RHE), which is 46.9 times higher than that of the benchmark Pt/C. The minimal performance decay after 50,000 potential cycles confirms a good stability. In situ vibrational spectroscopy investigations and theoretical calculations highlight that interstitial H atoms facilitate the direct dissociation of O 2 while single Pb atoms enhance O 2 adsorption strength. The electroactive PdPbH x metallenes is attributed to the up-shifted Pd-4 d orbitals induced by H and Pb atoms. This research supplies critical inspiration for developing highly efficient ORR electrocatalysts.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2026
·
IF 4.1
Li Xiang,Ling Ling,Chi Chaoxian,Ding Chuan-Fan
The development of a rapid analytical method for distinguishing chiral sulfur-containing compounds is essential due to the significant differences in biological activity exhibited by different enantiomers. This work presents a straightforward and rapid method for the chiral discrimination of D/L-homocysteine (D/L-Hcy), D/L-homocystine (D/L-Hcy 2 ), and S-allyl-D/L-cysteine (D/L-SAC) using cyclodextrin (CD) and metal adducts in conjunction with ion mobility-mass spectrometry (IM-MS). Through the formation of ternary complexes, separation was achieved with IM peak resolutions of 1.41 for [α-CD+D/L-Hcy+Co−H] + , 1.24 for [α-CD+D/L-Hcy 2 +Ca−H] + , and 1.65 for [β-CD+D/L-SAC+Fe−H] + . Theoretical calculations were performed to identify the optimal conformations of these ternary complexes. Conformational analysis elucidated distinct structural differences at the molecular level, providing insight into the chiral separation mechanism. Owing to its superior resolving power, this method enables the direct quantification of D/L enantiomers in mixtures. It was successfully applied to the determination of D/L-SAC in garlic samples, obviating the need for laborious sample pretreatment or derivatization steps. Overall, this work establishes a robust analytical platform for the chiral analysis of sulfur-containing amino acids and their derivatives. Graphical abstract
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Nature Communications
·
2026
·
IF 18.1
Luo Yaling,Chen Ruotian,Dittrich Thomas,Gao Pan,Ni Chenwei,Zhang Jie,Zhao Yue,Zhang Deyun,Ta Na,Li Mingrun,Zhang Mingjian,Li Dongfeng,Feng Zhendong,Li Zheng,Yu Yang,Zhou Panwang,Domen Kazunari,Fan Fengtao,Li Can
Photocatalytic overall water splitting remains limited by inefficient charge separation and utilization in reactions. Al-doped SrTiO 3 exhibiting near-100% apparent quantum efficiency for overall water splitting indicates nearly complete charge separation and surface catalytic efficiency. Although Al doping has been assumed to enhance charge separation and transfer, the exact role of Al is still unclear. Here, using spatiotemporal surface photovoltage imaging, we show that a gradient Al doping in Al-doped SrTiO 3 generates a built-in electric field that drives photogenerated holes from the bulk toward surface trap sites in the form of hydroxylated Al-O-Ti, prolonging their lifetime from ~100 ns to 10 ms. Spectroscopic analyses reveal that these hydroxylated Al sites serve as key centers for water adsorption, facilitating water oxidation. These findings underscore the pivotal role of Al in the spatiotemporal alignment of hole transfer and surface catalytic water oxidation, enabling high-efficiency photocatalysis in overall water splitting.
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Cell Death & Disease
·
2026
·Vol. 17
·期 1
·226
·
IF 12.2
Rao Yongjuan,Wang Yunran,Gao Jie,Guan Xun,Lv Sixuan,Gu Junlian,Wang Kefang
Overactive bladder (OAB) is strongly linked to intravesical inflammatory responses. Transient receptor potential ankyrin-1 (TRPA1) is a key sensor and signaling molecule in bladder function, crucial for initiating and maintaining inflammation. However, the mechanisms underlying TRPA1-mediated inflammatory processes in OAB remain unclear. This study aims to elucidate the molecular mechanisms of TRPA1 and the contribution of inflammatory pathways to OAB. Elevated TRPA1 expression was observed in urinary sediment cells from OAB patients and in bladder tissues from OAB animal models. Mechanistically, TRPA1 drives the progression of OAB by activating the Nod-Like Receptor Family Pyrin Domain Containing 3 (NLRP3) inflammasome and triggering pyroptosis. Notably, treatment with HC-030031 effectively mitigated inflammatory responses and restored bladder function, whereas Nlrp3 overexpression negated these therapeutic benefits. Furthermore, TRPA1-mediated upregulation of NLRP3 was dependent on the transcription factors MAZ and SMAD3, highlighting a novel regulatory axis. Our findings establish TRPA1 as a pivotal mediator in the progression of OAB by activating the NLRP3 inflammasome and thereby inducing pyroptosis. Targeting TRPA1 or the NLRP3 signaling pathway represents a promising therapeutic strategy for OAB, offering new insights into disease management and potential improvements in patient outcomes.
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Journal of Ovarian Research
·
2026
·
IF 5.3
Li Xingying,Hu Xueyuan,Yang Jing,Yue Xiuyong,Deng Lilin,Xiong Qian
Background Bushen Huatan Formula (BSHT) shows promise in treating reproductive endocrine disorders, yet its mechanism in polycystic ovary syndrome (PCOS) remains unclear. This study aims to elucidate the specific mechanism of action of BSHT in treating PCOS. Methods PCOS model in rats was established by using a high-fat diet combined with letrozole gavage, and an in vitro model was constructed by stimulating human ovarian granulosa cells with dehydroepiandrosterone. Oestrus cycle changes were monitored throughout the BSHT intervention period via daily vaginal smear. Following intervention, ovarian histopathology and serum sex hormones, lipid profiles, oxidative stress, and ferroptosis markers were assessed. Network pharmacology predicted BSHT targets and pathways, validated in vitro/in vivo via TP53 pathway expression and downstream factors, oxidative stress, and key ferroptosis protein levels. Results High-dose BSHT effectively improved estrous cycle irregularities, reduced ovarian and uterine indexes, and repaired ovarian pathology in PCOS rats. BSHT treatment significantly improved endocrine profiles by reducing testosterone and luteinizing hormone levels while increasing follicle-stimulating hormone ( P < 0.001), and ameliorated dyslipidemia by lowering total cholesterol and low-density lipoprotein cholesterol while elevating high-density lipoprotein cholesterol ( P < 0.001). Mechanistically, BSHT suppressed oxidative stress and ferroptosis, as evidenced by decreased malondialdehyde, reactive oxygen species, lipid peroxides, Fe2 + and ACSL4, along with increased GSH and GPX4 ( P < 0.001). Both network pharmacology and experimental validation confirmed that these effects were mediated through inhibition of the TP53 signaling pathway and its downstream targets ( P < 0.001). The critical role of TP53 was further verified through intervention studies using ferroptosis inhibitors and TP53 agonists ( P < 0.001). Conclusion This study demonstrates that BSHT alleviates PCOS pathology by inhibiting ferroptosis and regulating lipid metabolism via the TP53 signaling pathway, providing a theoretical basis for novel therapeutic strategies.
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Nature Communications
·
2026
·
IF 18.1
Lu Xulei,Liu Jialin,Fu Chunqiao,Duan Peng,He Changliu,Wang Chenxing,Zhang Yong,Huang Yinpeng,He Qi-Chang,Yang Tingting
Moisture-induced ion diffusion in nanostructured materials is a promising route for high-performance power generation, yet achieving the continuous ion migration necessary for long-term operation remains challenging. Here, we report a moisture-electricity conversion mechanism capable of sustained electrical output. We demonstrate that humidity fluctuations regulate the dynamic coordination between iodide ions (I - ) and iodine molecules (I 2 ), driving the oscillatory migration of I - to generate a continuous alternating ion current (AC). Crucially, this coordination process avoids charge exchange, while electricity generation is achieved through moisture-driven ion transport. The device achieves a current output of 33.2 µA cm - ² and exhibits sustainable performance recovery under natural humidity fluctuations. This mechanism remains effective even under minimal humidity gradients (13% RH), ensuring adaptability to diverse weather conditions. Our strategy, applicable to various gel materials, utilizes environmental humidity fluctuations as a power source for ion diffusion, offering a fundamental solution for long-term, autonomous energy harvesting.
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