WATER RESEARCH
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2023
·
IF 12.8
Qiang Liu,Xiaojun Niu,Dongqing Zhang,Xingyao Ye,Peibing Tan,Ting Shu,Zhang Lin
Phosphite, an essential component in the biogeochemical phosphorus cycle, may make significant contributions to the bioavailable phosphorus pool as well as water eutrophication. However, to date, the potential impacts of coexisting photochemically active substances on the environmental fate and transformation of phosphite in aquatic environments have been sparsely elucidated. In the present study, the effect of zinc oxide nanoparticles (ZnO NPs), a widely distributed photocatalyst in aquatic environments, on phosphite phototransformation under simulated solar irradiation was systematically investigated. The physicochemical characteristics of the pristine and reacted ZnO NPs were thoroughly characterized. The results showed that the presence of ZnO NPs induced the indirect phototransformation of phosphite to phosphate, and the reaction rate increased with increasing ZnO NPs concentration. Through experiments with quenching and trapping free radicals, it was proved that photogenerated reactive oxygen species (ROS), such as hydroxyl radical (•OH), superoxide anion (O 2 •− ), and singlet oxygen ( 1 O 2 ), made substantial contributions to phosphite phototransformation. In addition, the influencing factors such as initial phosphite concentration, pH, water matrixes (Cl − , F − , Br − , SO 4 2− , NO 3 − , NO 2 − , HCO 3 − , humic acid (HA) and citric acid (CA)) were investigated. The component of generated precipitates after the phosphite phototransformation induced by ZnO NPs was still dominated by ZnO NPs, while the presence of amorphous Zn 3 (PO 4 ) 2 was identified. This work explored ZnO NPs-mediated phosphite phototransformation processes, indicating that nanophotocatalysts released into aquatic environments such as ZnO NPs may function as photosensitizers to play a beneficial role in the transformation of phosphite to phosphate, thereby potentially mitigating the toxicity of phosphite to aquatic organisms while exacerbating eutrophication. The findings of this study provide a novel insight into the comprehensive assessment of the environmental fate, potential ecological risk, and biogeochemical behaviors of phosphite in natural aquatic environments under the condition of combined pollution.
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Small
·
2023
·
IF 13.3
Zhihao Chen,Malik Zeeshan Shahid,Xinyan Jiang,Meng Zhang,Danrui Pan,Hongpeng Xu,Guocan Jiang,Jin Wang,Zhengquan Li
Halide perovskites exhibit outstanding optoelectronic properties, which make them an ideal choice for photocatalytic CO 2 reduction and benzyl alcohol (BA) oxidation. Nevertheless, the simultaneous realization of the above redox coupling reactions on halide perovskites remains a great challenge, as it requires distinct catalytic sites for different target reactions. Herein, the catalytic sites of Cs 2 AgBiCl 6 (CABC) are regulated by doping Fe for efficient coupling of photocatalytic CO 2 reduction and BA oxidation. The Fe-doped CABC (Fe: CABC) exhibits an enhanced visible-light response and effective charge separation. Experimental results and theoretical calculations reveal a synergistic interplay between Bi and Fe sites, where the Bi and Fe sites have lower activation energies toward CO 2 reduction and BA oxidation. Further investigations demonstrate that electrons and holes prefer to accumulate at the Bi site and Fe site under light irradiation, respectively, which creates favorable conditions for facilitating CO 2 reduction and BA oxidation. The resultant Fe: CABC achieves a high photocatalytic performance toward CO (18.5 µmol g −1 h −1 ) and BD (1.1 mmol g −1 h −1 ) generation, which surpasses most of the state-of-the-art halide photocatalysts. This work demonstrates a facile strategy for regulating the catalytic site for redox coupling reactions, which will pave a new way for designing halide perovskites for photocatalysis.
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Antioxidants
·
2023
·Vol. 12
·期 9
·1685
·
IF 7
Lingyu Xie,Yibin Xu,Xiaoqing Ding,Kaixuan Li,Shuang Liang,Danlei Li,Yongxia Wang,Aikun Fu,Weixiang Yu,Xiuan Zhan
Earlier studies have shown that selenomethionine (SM) supplements in broiler breeders had higher deposition in eggs, further reduced the mortality of chicken embryos, and exerted a stronger antioxidant ability in offspring than sodium selenite (SS). Since previous studies also confirmed that Se deposition in eggs was positively correlated with maternal supplementation, this study aimed to directly investigate the antioxidant activities and underlying mechanisms of SS and SM on the chicken hepatocellular carcinoma cell line (LMH). The cytotoxicity results showed that the safe concentration of SM was up to 1000 ng/mL, while SS was 100 ng/mL. In Se treatments, both SS and SM significantly elevated mRNA stability and the protein synthesis rate of glutathione peroxidase (GPx) and thioredoxin reductase (TrxR), two Se-containing antioxidant enzymes. Furthermore, SM exerted protective effects in the H2O2-induced oxidant stress model by reducing free radicals (including ROS, MDA, and NO) and elevating the activities of antioxidative enzymes, which performed better than SS. Furthermore, the results showed that cotreatment with SM significantly induced apoptosis induced by H2O2on elevating the content of Bcl-2 and decreasing caspase-3. Moreover, investigations of the mechanism revealed that SM might exert antioxidant effects on H2O2-induced LMHs by activating the Nrf2 pathway and enhancing the activities of major antioxidant selenoenzymes downstream. These findings provide evidence for the effectiveness of SM on ameliorating H2O2-induced oxidative impairment and suggest SM has the potential to be used in the prevention or adjuvant treatment of oxidative-related impairment in poultry feeds.Keywords:selenomethionine;sodium selenite;antioxidants;glutathione peroxidase;Nrf2
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SENSORS AND ACTUATORS B-CHEMICAL
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2023
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IF 8.4
Ming Sui,Yanni Huang,Yang Tang,Jiaoyang Luo,Yiyang Dong,Yifei Guo,Yonggui Ma,Wei Gu,Mengyue Guo,Jingdan Huang,Dandan Kong,Jinping Li,Meihua Yang
Medicinal teas (MT) were popular in Asian countries for its various effective components. However, Pb pollution poses a serious threat to MT consumption due to the irreversible effect on enzyme activity and neurodegenerative disease. Owing to its toxicity and matrix interference, a rapid screening method for Pb at trace level with high sensitivity and accuracy is urgent for MT consumers. Herein, a simple and ultra-sensitive AuNPs/MWCNTs-MB/DNA-GCE quadruplex biosensor was developed and investigated. Specifically, the monitoring technology of scanning electrochemical microscope was utilized to analyze the assembly and application process for screening key parameters in complex matrix , through in-situ characterization of the microstructure changes on stepwise modified sensors. According to the combined results of electrochemical test and Randles-Sevcik equation, the proposed sensor showed a wide linear range from 5.0 * 10 −15 to 5.0 * 10 −13 M with LOD of 0.9 fM. In addition, the optimized sensor exhibited an excellent selectivity under the inference of metals and medicinal components. The proposed biosensor was successfully implemented for the ultrasensitive monitoring Pb pollution in MT while its water infusions recoveries maintained within acceptable range (81–118 %, 82–129 %). In all, AuNPs/MWCNTs-MB/DNA-GCE provided a new insight into the design, optimization, application and mechanism analysis of sensor for reliable sensing in controlling MT pollution and consumption safety.
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Science Advances
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2023
·Vol. 9
·期 25
·
IF 13.6
Zhou Zhu,Jian Wang,Xibo Pei,Junyu Chen,Xinwei Wei,Yanhua Liu,Pengcheng Xia,Qianbing Wan,Zhen Gu,Yong He
Intratissue topical medication is important for the treatment of cutaneous, mucosal or splanchnic diseases. However, penetrating surface barriers to providing adequate and controllable drug delivery while guaranteeing adhesion in bodily fluids remains challenging. Here, the predatory behavior of the blue-ringed octopus inspired us with a strategy to improve topical medication. For effective intratissue drug delivery, the active injection microneedles were prepared in a manner inspired by the teeth and venom secretion of blue-ringed octopus. With on demand release function guided by temperature-sensitive hydrophobic and shrinkage variations, these microneedles can supply adequate drug delivery at an early stage and then achieve the long-term release stage. Meanwhile, the bionic suction cups were developed to facilitate microneedles to stay firmly in place (>10 kilopascal) when wet. With wet bonding ability and multiple delivery mode, this microneedle patch achieved satisfactory efficacy, such as accelerating the ulcers’ healing speed or halting early tumor progression.
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FOLIA HISTOCHEMICA ET CYTOBIOLOGICA
·
2023
·Vol. 61
·期 2
·109-122
·
IF 1.5
Jiahui Han Di Jia Hao Yao Ting Lv Xi Xu Xin Ge
Introduction. Hemorrhagic shock (HS) is an important cause of high mortality in traumatized patients. Cryptotanshinone (CTS) is a bioactive compound extracted from Salvia miltiorrhiza Bunge (Danshen). The current study aimed to explore the effect and underlying mechanism of CTS on the liver injury induced by HS. Material and methods. Male Sprague-Dawley rats were used to establish the HS model by hemorrhaging and monitoring mean arterial pressure (MAP). CTS was intravenously administered at concentration of 3.5 mg/kg, 7 mg/kg, or 14 mg/kg 30 minutes before resuscitation. Twenty-four hours after resuscitation, the liver tissue and serum samples were collected for the following examinations. Hematoxylin and eosin (H&E) staining was used to evaluate hepatic morphology changes. The myeloperoxidase (MPO) activity in liver tissue and the serum activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were examined to reveal the extent of liver injury. The protein expression of Bax and Bcl-2 in liver tissue was detected by western blot. The TUNEL assay determined the apoptosis of hepatocytes. Oxidative stress of liver tissue was assessed by the examination of reactive oxygen species (ROS) generation. The content of malondialdehyde (MDA), glutathione (GSH), and adenosine triphosphate (ATP), the activity of superoxide dismutase (SOD) and oxidative chain complexes (complex I, II, III, IV), as well as cytochrome c expression in cytoplasm and mitochondria, were also used to determine the extent of oxidative injury in the liver. Immunofluorescence (IF) was employed to estimate nuclear factor E2-related factor 2 (Nrf2) expression. The mRNA and protein levels of heme oxygenase 1 (HO-1), NAD(P)H: quinone oxidoreductases 1 (NQO1), cyclooxygenase-2 (COX-2), and nitric oxide synthase (iNOS) were assessed by real-time qPCR, western blot to investigate the mechanism of CTS regulating HS-induced liver injury. Results. H&E staining and a histological score of rat liver suggested that HS induced liver injury. The activity of ALT, AST, and MPO was significantly increased by HS treatment. After CTS administration the ALT, AST, and MPO activities were suppressed, which indicates the liver injury was alleviated by CTS. The HS-induced upregulation of the TUNEL-positive cell rate was suppressed by various doses of CTS. HS-induced ROS production was decreased and the protein expression of Bax and Bcl-2 in the HS-induced rat liver was reversed by CTS administration. In the liver of HS-induced rats, the upregulation of MDA content and the downregulation of GSH content and SOD activity were suppressed by CTS. Additionally, CTS increases ATP content and mitochondrial oxidative complexes activities and suppressed the release of cytochrome c from mitochondria to the cytoplasm. Moreover, IF and western blot demonstrated that the activation of Nrf2 blocked by HS was recovered by different doses of CTS in liver tissue. The expression of downstream enzymes of the Nrf2 pathway, including HO-1, NQO1, COX-2, and iNOS, was reversed by CTS in the HS rat model. Conclusions. The current study for the first time revealed the protective effect of CTS in HS-induced liver injury. CTS effectively recovered hepatocyte apoptosis, oxidative stress, and mitochondria damage induced by HS in the rat liver partly via regulating the Nrf2 signaling pathway.
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Acta Biomaterialia
·
2023
·
IF 9.7
Xuan Meng,Lin Wang,Ning Zhao,Delong Zhao,Yongli Shen,Yuan Yao,Wenjie Jing,Shuli Man,Yujie Dai,Yanjun Zhao
The solid tumors are characterized with oxidative stress and metabolic reprogramming, which has been independently used for targeted tumor monotherapy. However, the potential of targeting metabolism-redox circuit in tumor therapy has long been neglected. Herein, we report a hybrid nanocarrier for concurrent targeting of glycolysis and redox balance in the current work. The nanocarriers are made of pH- and ATP-responsive zeolitic imidazolate framework (ZIF-8) as the porous core that was further coated with poloxamer 407 as the steric stabilizer . Two active cargos, glucose oxidase (GOx) and 3-bromopyruvate (3-BrPA) were co-loaded in the core of nanocarrier. GOx is well-known for its ability of producing hydrogen peroxide at the expense of glucose and oxygen. 3-BrPA can reduce oxygen and glucose consumption through glycolysis, which sensitized cancer cells to GOx-induced apoptosis. At the cellular level, the hybrid nanocarrier significantly impaired the redox balance in the liver hepatocellular carcinoma cell line (HepG2), as evidenced by the depletion of glutathione and boost of reactive oxygen species. The potency of hybrid nanocarrier in terms of suppressing HepG2 cell energy metabolism was proven by the exhaustion of ATP. As a consequence, cell viability was greatly reduced. The in vivo efficacy of hybrid nanocarriers was demonstrated in HepG2 tumor-bearing mice. The current work presents an approach of targeting metabolism-redox circuit for tumor treatment, which may enrich the available anti-tumor strategies. Statement of significance Metabolic alterations and elevated reactive oxygen species (ROS) are two characteristics of cancer. The metabolic patterns of cancer cells are elaborately reprogrammed to enable the rapid propagation of cancer cells. However, the potential of targeting the metabolism-redox circuit in anti-tumor therapy has long been neglected. As a proof-of-concept, we report an engineered stimuli-responsive nanomedicine that can eradicate cancer cells via cooperative glycolysis inhibition and redox impairment. The current work presents an approach of targeting the metabolism-redox circuit for tumor treatment, which may enrich the available anti-tumor strategies.
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APOPTOSIS
·
2023
·
IF 7.2
Yuan Qingling,Fan Yuxia,Liu Zheng,Wang Xiaoming,Jia Meng,Dong Yongqiang,Geng Zushi,Zheng Jian,Lu Xiubo
Thyroid cancer (TC) is one of the most common endocrine system cancers, and its incidence is elevating. There is an urgent need to develop a deeper understanding of TC pathogenesis and explore new therapeutic target for its treatment. This study aimed to investigate the effects of pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4) on the progression of TC. Herein, 29 pairs of TC and adjacent tissues were used to assess the expression of PLEKHG4. A xenograft model of mouse was established by subcutaneously injected with TC cells. Lung metastasis model was established through left ventricular injection. The results revealed that PLEKHG4 was up-regulated in human TC tissues. PLEKHG4 level was correlated with clinicopathological parameters of TC patients. In vitro assays revealed that PLEKHG4 promoted TC cell proliferation, migration, invasion, and epithelial-mesenchymal transformation. Knockdown of PLEKHG4 led to the opposite effects, and the loss of PLEKHG4 enhanced the apoptosis ability and inhibited the stemness properties of TC cells. These findings were further confirmed by the in vivo growth and lung metastasis of TC tumor. Mechanistically, PLEKHG4 promoted the activation of RhoGTPases RhoA, Cdc42, and Rac1. The inhibitors of these RhoGTPases reversed the PLEKHG4-induced malignant phenotypes. Additionally, ubiquitin-conjugating enzyme E2O (UBE2O), a large E2 ubiquitin-conjugating enzyme acted as an ubiquitin enzyme of PLEKHG4, facilitated its ubiquitination and degradation. In conclusion, PLEKHG4, regulated by UBE2O, promoted the thyroid cancer progression via activating the RhoGTPases pathway. UBE2O/PLEKHG4/RhoGTPases axis is expected to be a novel a therapeutic target for TC treatment.
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Gut Microbes
·
2023
·
IF 12.2
Jie Hu,Yifan Wu,Xingjian Zhou,Luyuan Kang,Shiyi Zhang,Yisi Liu,Yu Pi,Xilong Li,Junjun Wang,Dandan Han
The pathogenicity of Escherichia coli (E. coli) O157:H7 is predominantly associated with Shiga toxin 2 (Stx2) that poses a huge threat to human and animal intestinal health. Production of Stx2 requires expression of stx2 gene, which is located in the genome of lambdoid Stx2 prophage. Growing evidence has implicated that many commonly consumed foods participate in the regulation of prophage induction. In this study, we aimed to explore whether specific dietary functional sugars could inhibit Stx2 prophage induction in E. coli O157:H7, thereby preventing Stx2 production and promoting intestinal health. We demonstrated that Stx2 prophage induction in E. coli O157:H7 was strongly inhibited by L-arabinose both in vitro and in a mouse model. Mechanistically, L-arabinose at doses of 9, 12, or 15 mM diminished RecA protein levels, a master mediator of the SOS response, contributing to reduced Stx2-converting phage induction. L-Arabinose inhibited quorum sensing and oxidative stress response, which are known as positive regulators of the SOS response and subsequent Stx2 phage production. Furthermore, L-arabinose impaired E. coli O157:H7 arginine transport and metabolism that were involved in producing Stx2 phage. Collectively, our results suggest that L-arabinose may be exploited as a novel Stx2 prophage induction inhibitor against E. coli O157:H7 infection.
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CHEMICAL ENGINEERING JOURNAL
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2023
·
IF 15.1
Hui Yu,Haoyi Zhao,Yujie Zhang,Yuemin Hou,Runqing Li,Ting Liang,Yuanyuan Zhang,Cheng Li,Jingjie Zhao,Mingzhen Zhang,Ruifang An
Choriocarcinoma is a malignant tumor caused by aberrant proliferation and excessive invasion of trophoblastic cells in the uterus. Treatment for choriocarcinoma is hampered by severe side effects and drug resistance caused by insufficient delivery of chemotherapeutics. This dilemma can be solved by a novel approach of nanoparticle-based antineoplastic drug delivery through homologous targeting. Therefore, we developed a cancer cell membrane-coated biomimetic nanoreactor (MMC) built on a ferric metal–organic framework (MIL-100) encapsulating methotrexate (MTX) to achieve maximum therapeutic effects with negligible side effects. MMC could specifically target tumor cells and accumulate within tumor sites. MMC showed significant anticancer effects on JEG-3 cells, including inhibiting cell proliferation by cell cycle arrest at G 0 /G 1 stage, and reducing endocrine function and migration ability. Furthermore, OH overproduction by the Fenton reaction caused apoptosis and remarkable chemodynamic therapy (CDT) effects, glutathione scavenging led to intracellular redox dyshomeostasis and lipid peroxidation resulted in ferroptosis. In vivo studies demonstrated satisfactory tumor inhibition effects on tumor-bearing mice without appreciable toxicity. Further transcriptomics analysis revealed that MMC influences multiple pathways in cancer and cell death, including apoptosis and ferroptosis. This elaborately synthesized biomimetic nanoreactor offers a multifunctional reference for cancer therapy by presenting a new strategy for combining chemotherapy with CDT through synergistic apoptosis and ferroptosis.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2023
·Vol. 415
·期 20
·4935-4947
·
IF 4.3
Chen Yiyi,Shen Yanghong,Wang Haifeng,Zhang Jinzhi,Zhu Junli
Due to universal contamination and synergistic toxicity of multiple mycotoxins in foodstuff, reliable and high-throughput detection methods for multiple mycotoxins are urgently needed in corn products. In this study, a novel dual-channel immunochromatographic assay (ICA) based on improved up-conversion nanoparticles (IUCNPs) was developed for rapidly detecting aflatoxin B1 (AFB1) and zearalenone (ZEN). The synthesized IUCNPs doped by 30% Lu 3+ showed a larger size, more regular structure, and brighter fluorescence intensity than conventional UCNPs. The limits of detection (LODs) of single-channel ICA test strips for AFB1 and ZEN detection were 0.01 and 0.1 ng/mL, respectively. After the optimization, the dual-channel ICA of AFB1 and ZEN in 10 min was conducted, resulting in low detection limits of 0.025 and 0.1 ng/mL, respectively. Moreover, the built assay was revealed to be highly specific for six other food-contaminated mycotoxins, and exhibited excellent accuracy, with corresponding R 2 of 0.9931 and 0.9982 in calibration curves, respectively. Long-term storage experiments indicated that the dual-channel test strips had superior stability and precision. The LODs of AFB1 and ZEN in spiked maize were 0.025 and 0.25 μg/kg, demonstrating great sensitivity and matrix tolerance. Furthermore, the IUNCP-ICA was validated by high-performance liquid chromatography (HPLC) analyses, and a satisfactory consistency was obtained in 15 natural maize samples. Thus, the IUCNPs-ICA proposed in this work realized rapid and sensitive detection of AFB1 and ZEN, providing broad application potential in on-site screening for multiple mycotoxins in agricultural products. Graphical abstract
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Naymul Karim,Shiyu Liu,Ahmed K. Rashwan,Jiahong Xie,Jianling Mo,Ahmed I. Osman,David W. Rooney,Wei Chen
Anthocyanins are potential bioactive compounds with less bioavailability due to instability in physicochemical and physiological harsh environments. This study synthesized a “nanolipo-fibersomes (NLFS)” using Lipoid® S75 and Nutriose® FB 06 (dextrinization of wheat starch) through a self-assembly technique with probe sonication. We aimed to encapsulate delphinidin- 3-O -sambubioside (D3S) successfully and evaluate physicochemical and controlled release properties with improved antioxidant activity on palmitic acid (PA)-induced colonic cells (Caco-2 cells). D3S-loaded nanolipo-fibersomes (D3S-NLFS) were nanosized ( < 150 nm), spherical shaped, and homogenously dispersed in solution with promising encapsulation efficiency (~ 89.31 to 97.31 %). Particles formation was further verified by FTIR. NLFS were well-stable in thermal, storage, and gastrointestinal mimic environments. NLFS exhibited better-controlled release and mucoadhesive properties compared to nanoliposomes (NL). The NLFS showed better cellular uptake than NL, which was correlated to higher mucoadhesive properties. Furthermore, D3S-NLFS exhibited promising protective effects against PA-induced cytotoxicity, O 2 •− radicals generation, mitochondrial dysfunctions, and GSH depletion, while the free D3S was ineffective. Among D3S-loaded nanoparticles, D3S-NLFS 3 was the most efficient nanocarrier followed by D3S-NLFS 2, D3S-NLFS 1, and D3S-NL, respectively. The above data suggest that nanolipo-fibersomes can be considered as promising nanovesicles for improving colonic delivery of hydrophilic compounds with controlled release properties and greater antioxidant activity.
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