INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Gong Chen,Panyang Gu,Wenfang Wu,Yuan Yin,Liangyu Pan,Shu Huang,Wei Lin,Meichun Deng
Histone methylations play a crucial role in the development of neuropathic pain, and SET domain containing 2 (SETD2), a histone methyltransferase, serves as the sole tri-methylase known to catalyze H3K36me3 at the gene body. The N -methyl- d -aspartate receptor (NMDAR) is activated and mediates excitatory synaptic transmission in neuropathic pain. Nevertheless, the involvement of SETD2 in neuropathic pain and the specific regulatory mechanisms affecting NMDARs remain poorly understood. The expression levels of SETD2 were significantly decreased in the spinal cord and dorsal root ganglion (DRG) of rodents undergoing neuropathic pain induced by sciatic nerve chronic constrictive injury. Lentiviral shRNA-mediated SETD2 knockdown and conditional knockout in sensory neurons caused sustained NMDAR upregulation in DRG and spinal cord, which resulted in heightened neuronal excitability and increased pain hypersensitivity. SETD2 deficiency also led to reduced H3K36me3 deposition within the Grin1 (glutamate ionotropic receptor NMDA type subunit 1) gene body, thereby promoting aberrant transcription of the NMDARs subunit GluN1. The absence of SETD2 in the DRG potentiated neuronal excitability and increased presynaptic NMDAR activity in the spinal dorsal horn. Chromatin immunoprecipitation sequencing targeting H3K36me3 identified NFAT5 as a co-transcription factor in the transcriptional regulation of Grin1 . These findings highlight SETD2 as a key regulator in pain signal transmission and offered new perspectives on the development of analgesics through the targeted modulation of epigenetic mechanisms.
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yufei Zhang,Jing Jin,Daoxue Zhou,Huihui Liu,Jinmei Lu,Mingming Zheng
Covalent organic frameworks (COFs) are crystalline, porous organic materials that have significant potential as supports for enzyme immobilization. Nevertheless, the in situ preparation of biocatalysts during the COF formation process remains a considerable challenge. Herein, we developed a one-pot in situ preparation strategy. The immobilized lipase PS@TPB-TFPB COF-I was fabricated by mixing the polyvinylpyrrolidone (PVP)-lipase PS complex with precursors 1,3,5-tris(4-aminophenyl)benzene (TPB) and 1,3,5-tris(4-formylphenyl)benzene (TFPB) in acetonitrile catalyzed by acetic acid at room temperature for 48 h. The formation mechanism was systematically investigated using time-dependent microscopy techniques. PVP acts as a guiding reagent, controlling the morphological changes that occur during this process. Furthermore, the biocatalyst was employed in the kinetic resolution of racemic 1-phenylethanol, resulting in a significant enhancement in the conversion rate, with a range of 2.1 to 10.6 times higher compared to free PS at the same reaction time. The robust biocatalyst maintained high catalytic activity and enantioselectivity even after 10 cycles. The strategy described here is promising for lipase immobilization and expands the range of applications for COFs in biomanufacturing.
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CHEMICAL ENGINEERING SCIENCE
·
2024
·
IF 4.1
Jie Zheng,Zihui Qin,Shiping Wu,Jinxian Zhao,Jun Ren
A series of ZnO nanostructures with different morphologies were prepared with hydrothermal synthesis method by adjusting the NaOH/Zn 2+ molar ratio. The Au/ZnO catalysts were further synthesized and investigated in the oxidative esterification of ethylene glycol (EG) in methanol to methyl glycolate (MG). As compared with prismatic Au/ZnO(2.0) and fusiform-shaped Au/ZnO(8.0), the flaky-shaped Au/ZnO(3.5) catalyst shows a significantly decreased Au particle size of 3.4 nm as well as superior catalytic performance with the EG conversion of 94.5 % and the selectivity of 91.8 % towards MG. The characterization results imply that the reaction undergoes three successive steps including glycol oxidation (the rate-determining step), nucleophilic addition of glycolaldehyde with methanol and oxidative dehydrogenation of hemiacetal. The small Au nanoparticles and abundant defective surface oxygen species (O V ) promote the generation of activated oxygen species (O δ- ), which facilitate the cleavage of C-H bonds in alkoxide intermediate methylene (–CH 2 ) as well as hemiacetal aldehyde groups (–CHO) and thereby boost the formation of MG. Moreover, base sites adsorbed the alcohol to facilitate the O-H bond cleavage and acid sites adsorbed hemiacetal or intermediate alkoxide to drive the reaction. The strong interaction at the Au-ZnO interface affords the superior catalytic performance of Au/ZnO(3.5) in the oxidative esterification of EG in methanol to MG.
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MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 9
·1-11
·
IF 5.3
Lu Juan,Guo Yanjia,Shan Xiangyu,Song Yujia,Li Ruidan,Tian Li,Li Xuwen
A solid-state electrochemiluminescence (ECL) sensor was fabricated by immobilizing luminol, a classical luminescent reagent, on a Zn-Co-ZIF carbon fiber-modified electrode for the rapid and sensitive detection of procymidone (PCM) in vegetable samples. The sensor was created by sequentially modifying the glassy carbon electrode with Zn-Co-ZIF carbon fiber (Zn-Co-ZIF CNFs), Pt@Au NPs, and luminol. Zn-Co-ZIF CNFs, prepared through electrospinning and high-temperature pyrolysis, possessed a large specific surface area and porosity, making it suitable as carrier and electron transfer accelerator in the system. Pt@Au NPs demonstrated excellent catalytic activity, effectively enhancing the generation of active substances. The ECL signal was significantly amplified by the combination of Zn-Co-ZIF CNFs and Pt@Au NPs, which can subsequently be diminished by procymidone. The ECL intensity decreased proportionally with the addition of procymidone, displaying a linear relationship within the concentration range 1.0 × 10 –13 to 1.0 × 10 –6 mol L −1 (R 2 = 0.993). The sensor exhibited a detection limit of 3.3 × 10 –14 mol L −1 (S/N = 3) and demonstrated outstanding reproducibility and stability, making it well-suited for the detection of procymidone in vegetable samples. Graphical Abstract
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Chuanbo Ding,Jiali Yang,Ning Wang,Qiteng Ding,Shuwen Sun,Yang Gao,Liqian Shen,Ting Zhao,Yue Wang
Diabetic wounds are typically chronic wounds and the healing process is limited by problems such as high blood glucose levels, bacterial infections, and other issues that make wound healing difficult. Designing drug-loaded wound dressings is an effective way to promote diabetic wound healing. In this study, we developed an SA/PVA nanofiber (SPS) containing Shikonin (SK) for the treatment of diabetic wounds. The prepared nanofibers were uniform in diameter, had good hydrophilicity and high water vapor permeability, and effectively promoted gas exchange between the wound site and the outside world. The results of in vitro experiments showed that SPS was effective in antimicrobial, antioxidant, and biocompatible. In vivo tests showed that the wound healing rate of mice treated with SPS reached 85.5 %. Immunohistochemical staining results showed that SPS was involved in the diabetic wound healing process through the up-regulation of growth factors (CD31, HIF-1α) and the down-regulation of inflammatory factors (CD68). Western blotting experiments showed that SPS attenuated the inflammation through the inhibition of the IκBα/NF-κB signaling pathway. These results suggest that SPS is a promising candidate for future clinical application of chronic wound dressings.
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Materials Today Bio
·
2024
·
IF 8.7
Hebin Ma,Bowen Xie,Hongguang Chen,Lifang Hao,Haigang Jia,Dengjie Yu,Yuanbo Zhou,Puzhen Song,Yajing Li,Jing Liu,Kaitao Yu,Yantao Zhao,Yadong Zhang
A meniscus injury is a common cartilage disease of the knee joint. Despite the availability of various methods for the treatment of meniscal injuries, the poor regenerative capacity of the meniscus often necessitates resection, leading to the accelerated progression of osteoarthritis. Advances in tissue engineering have introduced meniscal tissue engineering as a potential treatment option. In this study, we established the size of a standardized meniscal scaffold using knee Magnetic Resonance Imaging (MRI) data and created a precise Polycaprolactone (PCL) scaffold utilizing 3-Dimensional (3D) printing technology, which was then combined with Fibrin (Fib) hydrogel to form a PCL-Fib scaffold. The PCL scaffold offers superior biomechanical properties, while the Fib hydrogel creates a conducive microenvironment for cell growth, supporting chondrocyte proliferation and extracellular matrix (ECM) production. Physical and chemical characterization, biocompatibility testing, and in vivo animal experiments revealed the excellent biomechanical properties and biocompatibility of the scaffold, which enhanced in situ meniscal regeneration and reduced osteoarthritis progression. In conclusion, the integration of 3D printing technology and the Fib hydrogel provided a supportive microenvironment for chondrocyte proliferation and ECM secretion, facilitating the in situ regeneration and repair of the meniscal defect. This innovative approach presents a promising avenue for meniscal injury treatment and advances the clinical utilization of artificial meniscal grafts.
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BIOCHEMICAL PHARMACOLOGY
·
2024
·
IF 5.3
Li Hu,Haiping Liu,Haixiang Ma,Xingyue Zeng,Yongkai Cao,Bing Liu,Huijun Li,Xiaohan Zhang
AKT, also known as protein kinase B (PKB), serves as a crucial regulator of numerous biological functions, including cell growth, metabolism, and tumorigenesis. Increasing evidence suggests that the kinase activity of AKT is regulated via ubiquitination by various E3 ligase enzymes in response to different stimuli. However, the molecular mechanisms underlying insulin-induced AKT ubiquitination are not yet fully understood. Here, we show that activation of the insulin receptor (IR) leads to enhanced ubiquitination of AKT1 at K8 and K14 residues, facilitated by the cytosolic E3 ubiquitin ligase enzyme, TRAF6. Further investigation using AKT1 mutants with modified nucleocytoplasmic shuttling properties reveals that TRAF6-mediated AKT1 ubiquitination occurs within the nucleus in a β-Arr2-dependent manner. The nuclear entry of TRAF6 depends on importin β1, while β-Arr2 regulates this process by facilitating the interaction between TRAF6 and importin β1. Additionally, the ubiquitination of AKT1 is essential for its translocation to the activated IR on the plasma membrane, where it plays a functional role in recruiting Glut4 and facilitating glucose uptake. This study uncovers the cellular components and processes involved in insulin-induced ubiquitination and activation of AKT1, providing insights and detailed strategies for manipulating AKT1.
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Zhen-Long Wang,Ke-An Wang,Xuechun Xiao,Hai-Bin Zhu
Amorphous and crystalline catalysts both have their own advantages to reduce the overpotential of OER. However, there is limited research on the relationship between the OER catalytic performance and the crystallinity degree. Herein, this key issue was explored by preparing FeW oxides with varying degrees of crystallinity. DFT combined with practical experimental results indicate that the change in crystalline state not only affects the electronic interactions in Fe-W-O but also the M-O bond lengths, both influencing the spin state of FeW oxides. At the critical temperature of amorphous-to-crystalline transition, a low-spin state of Fe in FeWO-450 is found to optimize the adsorption of intermediates during OER. FeWO-450 exhibits even better performance than commercial noble metal catalysts and numerous catalysts reported in the literature. This work showcases the effect of crystallinity of metal oxides on electron spin and offer a guidance how to improve OER properties by altering the electron spin states.
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SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Quantao Cui,Yuyin Dong,Weiwei Zou,Ziyu Song,Wei Zhang,Qiting Zuo,Xiaoli Zhao,Fengchang Wu
Sulfite-based advanced oxidation technology has received considerable attention for its application in organic pollutants elimination. However, the potential of natural sediments as effective catalysts for sulfite activation has been overlooked. This study investigates a novel process utilizing suspended sediment/sulfite (SS/S(IV)) for degradation of metronidazole (MNZ). Our results demonstrate that MNZ degradation efficiency can reach to 93.1 % within 90 min with 12.0 g SS and 2.0 mM sulfite. The influencing environmental factors, including initial pH, SS dosage, S(IV) concentration, temperature, and co-existing substances were systematically investigated. Quenching experiments and electron paramagnetic resonance analyses results indicate that SO 3 •− is the primary active substance responsible for MNZ degradation, with involvement of SO 4 •− , SO 5 •− , and • OH. X-ray photoelectron spectroscopy and Mössbauer spectra reveal that Fe (III)-silicates play a crucial role in activating S(IV). Furthermore, analysis of degradation intermediates and pathways of MNZ is conducted using liquid chromatography with mass spectrometry (LC -MS). The toxicity of MNZ and its intermediates were also systematically evaluated by the T.E.ST. program and wheat seeds germination test. This study offers valuable insight into the activation of sulfite by natural sediments and could contribute to the development of SS-based advanced oxidation processes (AOPs) for the in-situ remediation of antibiotics-contaminated water environments.
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Small
·
2025
·
IF 12.1
Xiaoyu Wang,Yangyang Zhao,Juntao Feng,Pei Wang,Xingchen Feng,Kai Yang,Xifeng Ding
Solid oxide electrolysis cells (SOECs) are promising energy conversion devices for the CO 2 reduction reaction (CO 2 RR). However, their applications are limited by sluggish reaction kinetics and poor durability of the electrode. Herein, a hetero-structured (PrBa) 0.95 Fe 1.5 Cu 0.5 O 6-δ with in situ formed FeCu bimetallic nanoparticles (FeCu@PB95FC) is designed to enhance CO 2 electrolysis and CH 4 reforming in SOECs. The current density of the cell with FeCu@PB95FC cathode achieves a remarkable current density of 1044.74 mA cm −2 at 800 °C and 1.5 V for pure CO 2 electrolysis, with a CO production rate of 6.4 mL min −1 cm −2 and a Faraday efficiency of 95.23%. The superior electrocatalytic activity is attributed to the exsolved nanoparticles promoting electron transfer and additional oxygen vacancies generated within the perovskite substrate, providing numerous metal-oxide interfaces for gas adsorption and electrolysis. Furthermore, to reduce energy consumption of CO 2 electrolysis while generating value-added products, the symmetrical cell (FeCu@PB95FC|LSGM|FeCu@PB95FC) is synthesized, achieving a current density of 1.54 A cm −2 , alongside high syngas production (CO: 4.49 mL min −1 cm −2 ; H₂: 6.53 mL min −1 cm −2 ). This work offers a synergistic strategy for designing robust electrodes for efficient CO 2 conversion and methane utilization in SOECs, advancing sustainable energy technologies.
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Fishes
·
2025
·Vol. 10
·期 3
·116
·
IF 2.4
Qikai Liu,Yang Liu,Yaqi Mao,Ru Li,Yujie Jiao,Jiali Lei,Fenzhen Zhang
The degradation of organic matter using TiO2nanotube photocatalytic technology is limited by the short lifetime and diffusion radius of the generated hydroxyl radicals, decreasing the removal efficiency. This study developed a chlorine radical-coupled photoelectrocatalytic system, significantly enhancing the performance of TiO2nanotube arrays in removing sulfonamide antibiotics (SAs) from cold-water aquaculture systems. The highest degradation rates were observed at 5 mM NaCl and 15 mM NaNO3. When SA concentrations were 0.1–10 mg/L, degradation efficiency decreased with higher initial concentrations. The best degradation was achieved at an initial pH of 3 for SA. Humic acid and sodium acetate, natural organic matter in the water column, served as low-concentration promoters and high-concentration inhibitors. In our study, three degradation intermediates were identified, and hydrolysis and nitration reactions are proposed as the primary pathways for SA degradation. We confirmed that oxygen radicals play a major role in this system. Furthermore, toxicology experiments revealed the weakening of the toxicity of the degraded products. This study provides an efficient method for treating organic matter in cold-water fish culture water in chloride-containing saline and alkaline waters.
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Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Zhifeng Li,Haomiao Cao,Zhaoyi Yin,Xiaoyi Dong,Jinge Hao,Zhiguo Song,Zhengwen Yang,Jianbei Qiu,Yongjin Li
Harvesting near-infrared (NIR) light to address the energy crisis and environmental pollution remains a challenge. In this study, full-spectrum-driven photocatalysts were realized through the design of Er 3+ /Yb 3+ co-doped Bi 3 Ti 2 O 8 F nanoplates. The Bi 3 Ti 2 O 8 F: Yb 3+ , Er 3+ nanoplates, when excited at 980 nm, demonstrated nearly single-band red upconversion (UC) emission. Furthermore, the corresponding UC emission mechanism was elucidated through the relationship between pump power and the UC emission spectrum. The experimental results and DFT calculation analysis revealed that the Er 3+ dopant ions form an impurity energy level, which broadens the light photoresponse range and promotes the separation of electron-hole (e − /h + ) pairs. Consequently, Bi 3 Ti 2 O 8 F:Yb 3+ , Er 3+ nanoplates exhibit 1.42 times better photocatalytic degradation of ciprofloxacin (CIP) than Bi 3 Ti 2 O 8 F under full-spectrum light irradiation and 4.54 times better than Bi 3 Ti 2 O 8 F: Er 3+ under NIR light irradiation. The trapping experiments and EPR analysis reveal that •O 2 – and h + are the main active species during the photocatalytic reaction process. Thus, this work demonstrates that efficient bismuth-based broadband photocatalysts can be developed through the simultaneous application of UC emission and doping engineering.
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