Food and Bioprocess Technology
·
2018
·Vol. 11
·期 10
·1853-1862
·
IF 2.998
Sun Zhidong,Hao Jiasi,Yang Huqing,Chen Huiyun
The effects of lauroyl arginate ethyl (LAE), chitosan (CTS), and CTS-containing montmorillonite and LAE coatings (CML) on the quality maintenance and shelf life of minimally processed grapes stored at 4 °C for 20 days were investigated. Compared with the control, LAE, and pure CTS coatings, the CML coatings effectively decreased grape weight loss, respiration, and decay incidence as well as microorganism counts of total aerobic bacteria, yeasts, molds, and lactic acid bacteria in grapes. The CML coatings also showed better effects on delaying the tissue softening and browning due to the decreased activities of polygalacturonase, pectin methyl esterase, and polyphenol oxidase. CML coatings did not significantly change the content of soluble solids and the titratable acidity or the pH value of grapes, compared with other samples. However, CML coatings ensured higher content of ascorbic acid and sensory quality in grapes. The results confirmed that CML coatings improve microbiological safety and preserve nutritional and sensory quality of minimally processed grapes.
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Advanced Healthcare Materials
·
2022
·Vol. 11
·期 15
·2200386
·
IF 11.092
Jile Xie,Jiaying Li,Jinjin Ma,Meimei Li,Xingran Wang,Xinya Fu,Yanxia Ma,Huilin Yang,Bin Li,Saijilafu
Because of the limited regenerative ability of the central nervous system (CNS), effective treatments for spinal cord injury (SCI) are still lacking. After SCI, neuron loss and axon regeneration failure often result in irreversible functional impairment. The calcium overload induced by the N-methyl-D-aspartate receptor (NMDAR) overactivation is critical for cell death in SCI. It has been reported that the magnesium ion (Mg 2+ ) can competitively block the NMDAR and reduce the calcium influx, and that sonic hedgehog (Shh) and retinoic acid (RA) are the critical regulators of neuronal differentiation of endogenous neural stem cells (NSCs). Here, magnesium oxide (MgO)/poly ( l -lactide-co- ε -caprolactone) (PLCL) scaffold loaded with purmorphamine (PUR, a Shh signaling agonist) and RA is developed and its feasibility in SCI repair is tested. The results showed that the Mg 2+ released from MgO attenuated cell apoptosis by blocking the calcium influx, and the PUR/RA promoted the recruitment and neuronal differentiation of endogenous NSCs, thereby reducing the glial scar formation at the SCI lesion site. Furthermore, implantation of PUR/RA-loaded MgO/PLCL scaffold facilitates the partial recovery of a locomotor function of SCI mouse in vivo. Together, findings from this study imply that PUR/RA-loaded MgO/PLCL scaffold may be a promising biomaterial for the clinical treatment of SCI.
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Journal of Biomedical Nanotechnology
·
2014
·Vol. 10
·期 6
·948-958
·
IF 7.578
Liu Yongjun,Liu Chunxi,Li Min,Liu Fengxi,Feng Lixia,Zhang Li,Zhang Na
Multifunctional nanoparticles assembled from multi-block polymers are now one of the most convenient and convincing carriers for target drug delivery. Multi-block polymers could provide multi-functions such as sufficient drug loading capability and efficient target ligand coupling potency.
In this article, novel multi-block polymer poly(lactic acid)–poly(ethylene glycol)–poly(L-lysine) (PLA-PEG-PLL) with relatively precise block molecular weight were synthesized by new method which we called polymer–polymer conjugation. This method conjugated different polymer
blocks by reactions between the terminal active groups of different blocks, thus simplified the synthesis procedure. The obtained PLA-PEG-PLL was characterized by 1H NMR and gel permeation chromatography. The controlled drug delivery capability and the target ligand coupling potency
of PLA-PEG-PLL were verified using paclitaxel (PTX) as model drug and vascular endothelial growth factor (VEGF) antibody as target ligand. The PTX-loaded PLA-PEG-PLL nanoparticles (PNP) and VEGF antibody modified PTX-loaded PLA-PEG-PLL nanoparticles (VPNP) were prepared using solvent diffusion
methods. The two nanoparticles showed spherical or ellipsoidal shapes with uniform particle size distribution (190.1 ± 1.27 nm and 203.6 ± 4.10 nm for PNP and VPNP, respectively) and positive zeta potential (23.76 ± 0.72 mv and 20.76 ± 0.34 mv for PNP and VPNP,
respectively). The cellular cytotoxicity, cellular uptake, in vivo therapeutic effects of the two nanoparticles were investigated. Cytotoxicity of VPNP against HepG2 cells was superior to that of PNP and Taxol®. The VPNP and PNP showed better antitumor efficacy in a murine model
bearing H22 compared with Taxol® and VPNP was the best. The study on cellular uptake indicated that the better antitumor efficacy of VPNP was attributed to the increased uptake of drug by tumor cells. These results demonstrated that PLA-PEG-PLL was a favorable multifunctional
material for drug target delivery and polymer–polymer conjugation was a promising method to fabricate novel multi-block polymers.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Jie Cao,Jingge Niu,Yizhuo Pang,Tianshu Luo,Jiayi Peng,Xiaohong Hou
Nitrite is a kind of frequently detected inorganic pollutants in water. In order to sensitively and conveniently monitor the nitrite anytime and anywhere, colorimetric sensing method-based nanoenzymes and portably immediate detection devices become urgent. Herein, we utilized L-arginine as a catalyst and a linker to mediate the synthesis of CeO 2 on bimetallic MOFs (CeO 2 @Mn/ZIF-67), with Co and Mn were set as the central metals. Its enzyme-activated performance ( K m : 0.054 mM and V max : 64.00 μMs −1 ) was superior to most reported materials. Nitrite can diazotize with oxTMB, causing a change in the ratio of absorbance at 652 nm to absorbance at 450 nm (A 652 /A 450 ). Thus, a rapid and sensitive platform for the colourimetric detection of nitrite was established. A portable glass tube hydrogel sensor was designed inspired by the principle of disposable alcohol swabs for the first time to achieve the nitrite monitoring. When the glass tube was cut to introduce TMB and nitrite into the nanoenzymes, a noticeable colour change occurred. Attributed to the high activity of the nanoenzymes and the clever design of the detection device, the proposed method overcame the shortcomings of the traditional sensing methods such as long detection time and poor portability.
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Biochemistry and Biophysics Reports
·
2026
·
IF 3.3
Wenyuan Wan,Yuntao Zhang,Xiaotong Yang,Jinyao Li,Jun Lu,Yu Zhao
This study investigated the reparative effects of Brassica rapa L. ethanolic extract (BREE) on H 2 O 2 -induced oxidative injury in PC-12 cells. Brassica rapa L. , a cruciferous plant rich in bioactive flavonoids, exhibits potent antioxidant and neuroprotective properties against oxidative stress, a key pathological driver in neurodegenerative diseases. This study investigated the reparative effects of Brassica rapa L. ethanolic extract (BREE) on H 2 O 2 -induced oxidative injury in PC-12 cells, employing a multi-omics approach integrating LC-MS/MS metabolite profiling, molecular docking, and transcriptomic validation. BREE demonstrated robust radical-scavenging activity. Molecular docking identified direct interactions among flavonoids within BREE, suggesting a role in redox modulation. Subsequent experiments confirmed the activation of the PI3K/Akt, Keap1-Nrf2, and cell cycle pathways. Functionally, BREE significantly restored cell viability to 162.3% ± 6.9% of that in the control group. These findings validate BREE's dual action in scavenging ROS and reprogramming redox signaling networks, positioning it as a candidate for combating oxidative stress-associated neurodegeneration and as a potential functional food for age-related cognitive decline.
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JOURNAL OF APPLIED POLYMER SCIENCE
·
2026
·Vol. 143
·期 15
·e70470
·
IF 3.1
Biqin Chen,Yuxia Li,Jinghao Cao,Shenghui Liu,Ke Fan,Haixin Li
The existing TUF membranes often face challenges such as declining permeation flux and insufficient separation selectivity. This study proposes to improve the above problems by the synergistic effect of Mg–Al layered double hydroxide (LDH) and tannic acid (TA) etched metal–organic frameworks (MOFs): (1) TUF membranes with adjustable interlayer distances and multi-level mass transfer channels were constructed by doping LDH two-dimensional (2D) nanosheets; (2) TAZIF-L (Co) etched by TA was introduced. TA inhibited the swelling of LDH nanosheets through coordination and enhanced the interface binding between ZIF-L (Co) and the substrate, thereby suppressing its shedding. The results showed that TA can etch ZIF-L (Co), and the membrane prepared after etching has better selectivity and stability of dye/salt separation. Taking MTAZIF-0.02 as an example, the pure water flux was 88.23 L m −2 h −1 , which was 1.91 times that of MLDH. The removal rate of congo red (CR) solution was 92.56%. The NaCl rejection in methyl blue (MB)/NaCl solution was 10.88% and in CR/NaCl solution was 10.57%. This study provides an experimental basis for the construction of highly selective ultrafiltration membrane for the treatment of high concentration dye/salt wastewater.
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JOURNAL OF HAZARDOUS MATERIALS
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2025
·
IF 11.3
Jiaqi Hu,Mingyue Wang,Min Wang,Dandan Wang,Yuqiang Xiao,Yue Lan,Lingling Li,Meiling Yang,Minmin Wang,Leijiao Peng,Famin Ke,Xiurong Guo,Die Gao
Considering the efficient utilization of resources and the simultaneous detection of multiple analytes, achieving selective detection of diverse analytes on one fluorescent covalent organic framework (COF) is of great significance. Herein, a carboxyl-carbazole-rich fluorescent COF (COF-DABT) was strategically synthesized using 3,6-diamino carbazole, capable of forming halogen bonds with analytes, and 2,5-bis(4′-formylphenyl) terephthalic acid, rich in carboxyl groups, which interacts with various analytes and adjusts fluorescence performance via its electron-withdrawing properties. The introduction of carbazole imparts fluorescence to COF-DABT through the intramolecular charge transfer (ICT) effect, while enabling selective fluorescence quenching. Specifically, the ICT of carbazole can be disrupted via linear halogen bond interactions with TBBPS, resulting in selectively detection of TBBPS in a fluorescence-off mode. Furthermore, efficient enrichment of TBBPS is facilitated by halogen bonds with carbazole and hydrogen bonds with carboxyl groups on COF-DABT. Selective fluorescence quenching of COF-DABT by Fe 3 + is achieved through chelating effect around carboxyl group, as well as cation-π interaction with carbazole benzene ring. This enables high selectivity detection of Fe 3+ via a fluorescence-off mode. Additionally, Fe 3+ can be reduced by ascorbic acid through REDOX reaction, mitigating interference with TBBPS detection and facilitating fluorescence-off-on mode detection of AA. Besides, rich carboxyl groups on COF-DABT exert a negative regulatory effect on the carbazole ICT mechanism. To counter this, D/L-Arg, with a size complementary to the adjacent carboxyl groups on COF-DABT, is introduced. Hydrogen bond interactions between D/L-Arg and carboxyl groups reduce the negative regulatory effect, allowing fluorescence enhancement for highly selective detection of D/L-Arg. Through rational design and functional optimization of fluorescent COF structures, multi-mode detection methods enable simultaneous detection of different analytes, achieving the goal of "one stone, several birds."
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JOURNAL OF CHROMATOGRAPHIC SCIENCE
·
2023
·
IF 1.3
Xia Chenjie,Zhu Hongliu,Hu Changhui,Liu Jie,Hua Haibing,Yang Jianqiu,Chen Zhipeng,Gao Yihong
Pinus massoniana needles, a traditional herb, were applied to prevent hair loss in China. Studies available mainly focused on pine needle flavonoids with various biological activities. However, there has been no pharmacokinetics study of the flavonoids from Pinus needles extract. A selective and sensitive ultra-high performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS) method was developed to simultaneously quantify taxifolin, quercetin and catechin in rat plasma. To separate the three constituents, an Agilent Extend-C18 column (2.1 mm × 100 mm, 1.8 μm) was used with a mobile phrase of (A) 0.1% formic acid and (B) acetonitrile. The analytes were measured by multiple reaction monitoring in the negative ionization mode. There was good linearity in the established UHPLC-MS/MS method, with a coefficient of determination (r2) of >0.99. The accuracy, intra-day and inter-day precision and recovery were all satisfactory and these 3 compounds were stable under the tested conditions. The validated method in this study was successfully applied to pharmacokinetic study in healthy rats after oral and transdermal administration of Pinus needles extract. The results could provide further research foundation for pine needle extract as external preparations.
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JOURNAL OF CHROMATOGRAPHY A
·
2022
·
IF 4.601
Meilin Xu,Lunzhao Yi,Dabing Ren,Boyan Li
Citrus flavonoids are attracting great interest due to their well-known beneficial effects, but many of them have not been characterized. In this work, ultra-high liquid chromatography coupled to high resolution mass spectrometry (UHPLC HRMS) was used for profiling flavonoids in citrus fruit. We proposed a strategy combining mass defect filtering (MDF) and MS/MS–based molecular networking (MMN) to handle complex UHPLC–HRMS data. The proposed strategy was explained and validated in the fruit of Citrus sinensis ( L. ) Osbeck, and when specific mass and mass defect windows were pre-defined, MDF enable removal of considerable un-related and/or interference MS 1 peaks. In citrus fruit, the number of MS 1 peaks in positive and negative modes were reduced by 70.80% (from 15,113 to 4413) and 55.30% (from 5617 to 2511), respectively, and thus the potential MS features of flavonoids were retained and exposed. After MDF, an MS/MS similarity-based MMN map was constructed to cluster flavonoids with similar chemical structures. MMN facilitated the annotation of 65 unknown citrus flavonoids by using only 21 pre-identified flavonoids as references. The compounds comprised 42 polymethoxylated flavonoids, 17 flavones, 24 flavanones, and 3 flavonols. Eleven of them had not been previously reported in Citrus sinensis ( L. ) Osbeck to our knowledge. Results of the current work indicated that the combination of MDF and MMN is a useful strategy for removing interference MS 1 peaks and performing the structural annotation of unkonwn compounds in complex samples.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Daiyan Wu,Ziwei Liu,Yangyang Feng,Feng Tang,Sheng Li,Xinnan Zhang,Hangyu Li,Qianqian Liu,Linzi Zhang,Qun Liu,Xiaonong Yang,Haibo Feng
Potentilla anserina L polysaccharide (PAP) is known to regulate immunity. Poly(lactic- co -glycolicacid) (PLGA) is a type of drug carrier with biocompatibility and biodegradable USFDA approved polymer, which possesses the advantages of high safety and good sustained-release effect. The DEC205 receptor, a type I membrane protein, is widely distributed on the surface of macrophages and dendritic cells (DCs) and plays a key role in antigen recognition and presentation. In this study, we prepared Potentilla anserina L polysaccharide PLGA nanoparticles targeting DEC205 receptor (DEC205-PAPP) and characterized the nanoparticles with regards to their effects on immune activation in vitro and in vivo . In vitro , DEC205-PAPP promoted the uptake activity of macrophages and increased the secretion of NO and cytokines (IFN-γ, IL-4, IL-6, and GM-CSF), up-regulated the expression of CD80 + , CD86 + . In vivo , DEC205-PAPP elevated the immune organ index, induced DC maturation, promoted T lymphocyte proliferation and differentiation, and increased the levels of antigen-specific IgG antibody and cytokines (IFN-γ, IL-4), which prolonged the residence time of the OVA antigen in the immune organs and the lymph nodes. In conclusion, DEC205-PAPP had a slow-release effect, induced humoral and cellular immune responses, and could potentially be used as an effective antigen-targeted delivery system.
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Drug Design Development and Therapy
·
2023
·
IF 4.8
Mukaram Amatjan,Na Li,Pengke He,Boheng Zhang,Xianyan Mai,Qianle Jiang,Haochen Xie,Xiaoni Shao
Background Cichorium intybus L. formula (CILF) is a traditional Chinese medicine (TCM) widely used in the treatment of gout and hyperuricemic nephropathy (HN). The aim of this research was to investigate the potential protective effect of CILF against HN and elucidated the underlying mechanism.Methods CILF water extract was administered to an HN rat model established by adenine combined with ethambutol. The levels of uric acid (UA), serum urea nitrogen (UREA), and creatinine (CREA) were detected. Changes in the pathology and histology of the kidney were observed by hematoxylin-eosin staining. The 16S rRNA of the gut microbiota was sequenced. The binding ability of the main ingredients of CILF to key targets was analyzed by network pharmacology and molecular docking. The expression levels of the related mRNAs and proteins in the kidney were evaluated by RT-qPCR and immunohistochemistry analysis.Results CILF administration significantly alleviated increases in UA, UREA, and CREA, structural damage, and kidney dysfunction. Gut microbiota analysis was applied to explore the pharmacological mechanism of the effects of CILF on bacterial diversity and microbiota structure in HN. CILF decreased the abundance of Bacteroides. In addition, it increased the abundance of Lactobacillaceae, Erysipelotrichaceae, Lachnospiraceae, Ruminococcaceae, and Bifidobacterium. Based on network pharmacology and molecular docking analysis, CILF profoundly influenced the IL17, TNF and AGE-RAGE signaling pathway. Additionally, CILF inhibited the expression of STAT3, VEGFA and SIRT1 to improve the symptoms of nephropathy. Our research suggested that CILF protects against kidney dysfunction in rats with HN induced by adenine combined with ethambutol.Conclusion Our findings on the anti-HN effects of CILF and its mechanism of action, from the viewpoint of systems biology, and elaborated that CILF can alter the diversity and community structure of the gut microbiota in HN, providing new approaches for the prevention and treatment of HN.
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PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2020
·
IF 2.751
Mei-Mei Wang,Ming He,Hong Wang,Yun-Feng Ma,Youssef Dewer,Fan Zhang,Peng He
Insect antennae play a fundamental role in perceiving and recognizing a broad spectrum of conventional semiochemicals and host plant-derived odors. As such, genes that are tightly associated with the antennae are thought to have olfactory-related roles related to signal transduction mechanisms. Several mechanisms suggest that enzymatic inactivation could contribute to the signal termination process, such as odorant-degrading enzymes (ODEs). To date, a few ODEs have been identified and characterized in detail in insect herbivores, but little is known about aldehyde oxidases (AOXs); moreover, direct in vivo experimental evidence is needed. AOXs are a major family of metabolic enzymes that oxidize a variety of aromatic aldehydes, and they may also play a significant role in detoxification and degradation of environmental chemical cues. Here, we report on the identification and characterization of a novel cDNA encoding the putative odorant-degrading enzyme, PxylAOX3, from the antennae of the diamondback moth , (DBM), Plutella xylostella (L.) (Lepidoptera: Plutellidae). The purified recombinant protein showed a wide-range of substrate zymography oxidizing both sex pheromone compounds as well as plant-derived aldehydes with distinct activities. Our data suggest PxylAOX3 might be involved in the degradation of many structurally diverse aldehyde odorants. Furthermore, PxylAOX3 could participate in olfactory neuron protection by inactivation of redundant odorants and xenobiotic detoxification, making it a potential target for pesticide development as well.
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危险品化学品经营许可证（带存储）