Stem Cell Reviews and Reports
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2026
·
IF 4.2
Yue Wei,Yang Yue,Miao Yan,Li Jinqi,Li Shanshan,Yang Yi,Zhang Yingwen,Qian Baohua,Li Yanxin,Gu Haihui
Objective The global shortage of platelets presents a significant challenge in healthcare. Although human induced pluripotent stem cells (hiPSCs) offer a renewable source for ex vivo platelet production, the current approach remains constrained by heterogeneity, low yield, and high costs. This study introduces an optimized differentiation scheme (ODS) to improve ex vivo platelet differentiation from hiPSCs. Methods A systematically optimized culture protocol was developed, incorporating: (1) a higher initial dose of embryoid body (EB) cells, (2) refining culture medium, (3) substitution of cytokines with small molecules, and (4) enhancement of megakaryocyte (MK) polyploidization via small-molecule supplementation. Feasibility and effectiveness were evaluated using microscopy, cell counting, flow cytometry, Wright-Giemsa staining, immunofluorescence (IF), and transmission electron microscopy (TEM). Results Increasing the initial EB cell count significantly promoted megakaryocyte production and accelerated the process. A serum-free medium supplemented with human platelet lysate (HPL) was favorable for megakaryocyte generation. Small molecules 740Y-P and butyzamide effectively substituted SCF and TPO for differentiation, while the combination of blebbistatin and 616452 enhanced megakaryocyte maturation. Mature megakaryocytes continuously generated functional platelets that, upon thrombin activation, facilitated fibrin clot formation and contraction in vitro. This method shortened differentiation to 19 days, enhanced output to 1.42 CD41⁺ megakaryocytes and 14.9 platelets per iPSC, and reduced costs by 58.3%. Conclusion We have established a cost-effective strategy for platelet production via hiPSC differentiation, with potential applications in cell therapy and gene editing. Graphical
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Nature Communications
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2026
·
IF 15.7
Xu Qingyang,Luo Kangwei,Wang Yipu,Wu Jiajie,Lu Hongwei,Chen Manyu,Xiong Zuping,Sun Jing Zhi,Zhang Jianyu,Huang Feihe,Tang Ben Zhong,Zhang Haoke
Multi-axis atropisomers are ubiquitous in nature, and their intricate stereoconformations not only deepen stereochemical understanding but also elucidate fundamental principles of molecular atropisomeric dynamics. Nevertheless, insights into multi-axis atropisomerism remain limited. This work presents a systematic investigation of three classes of LaPlente-type biaxial atropisomers based on naphthalene rotors and phenyl linkers. Thermodynamically and kinetically controlled interconversion of molecular conformations is achieved in 22-NB, 11-NB, and 11-NB-8DMe. Concurrently, a direct correlation between molecular dynamics and electronic through-space conjugation (TSC) is elucidated. Furthermore, absolute separation of syn and anti conformers is accomplished in 11-NB-2DMe, whose distinct TSC-induced fluorescence enables direct visualization of dynamic conformer populations and kinetics during temporally sequential crystallization. This work provides fundamental mechanistic insights into multi-axis atropisomer stereochemistry and establishes a fluorescence-based approach as an in-situ platform for real-time analysis of molecular dynamics across life and materials sciences.
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MOLECULAR AND CELLULAR BIOCHEMISTRY
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2026
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IF 3.7
Shi Chenjun,Lu Conghua,Lin Caiyu,Lian Shaopan,Luo Huanyu,You Ziyin,Li Li
Defective alveolar re-epithelialization following acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) represents a critical barrier to clinical recovery. Intrinsic signals that could be harnessed to speed this repair remain poorly defined. Endogenous electric fields (EFs) arise immediately after epithelial injury and are recognized as mediators of cell migration and morphogenesis, but their impact on lung epithelial proliferation is unknown. Here we demonstrate that physiological-strength direct-current EFs (100–200 mV/mm) are potent pro-mitogenic signals for both human bronchial (BEAS-2B) and murine alveolar (MLE-12) epithelial cells. Within a custom live-cell electrotactic chamber, EFs exposure for 4 h doubled EdU incorporation, increased Ki-67 expression, and elevated real-time mitotic events without altering spindle orientation. RNA-seq analysis of BEAS-2B cells with EFs exposure identified 1,447 differentially expressed genes, with significant enrichment of the PI3K/AKT signaling pathway and immunoblotting further confirmed rapid phosphorylation of PI3K p85 (Tyr458)/p55 (Tyr199) and AKT (Ser473). Selective inhibition with Alpelisib or LY294002 abolished pathway activation and fully suppressed the pro-proliferative effect of EFs. Furthermore, EFs triggered AKT-dependent phosphorylation of glycogen synthase kinase-3β (GSK3β) at Ser9, while blockade with the GSK3β inhibitor Tideglusib or upstream PI3K inhibitors suppressed this event and attenuated EF-induced proliferation. Taken together, our findings delineate the PI3K/AKT/GSK3β signaling axis through which EFs enhance pulmonary epithelial proliferation. This work identifies physiological EFs as previously unrecognized regenerative cues in the lung and underscores bioelectric modulation as a promising therapeutic strategy to accelerate alveolar repair in ALI/ARDS.
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Scientific Reports
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2026
·
IF 3.9
Zhang Fangxia,Luo Kunshun,Xuan Shaoyan,Chen Teng,Chen Zhiyong,Yang Jing,Zhou Ying,Feng Tingting,Zhu Yue,Wang Zuhua
Pancreatic cancer has a dismal prognosis due to the rapid progression and inefficiency of chemotherapy. To ameliorate this inefficiency of chemotherapy drug, we developed a long-circulating PEGylated nanoliposome (ORI@PD-Lipo) with 1,2-distearoylphosphoethanolamine- polyethyleneglycol-amine (DSPE-PEG 2000 -NH 2 ) to deliver oridonin, one of the active ingredients in Rabdosia rubescens ( R. rubescens ) in this study. For improving tumor-targeting delivery of oridonin, a pancreatic cancer targeting CKAAKN peptide was used to modify the nanoliposome (ORI@CPD-Lipo). Our results showed that ORI@CPD-Lipo had good stability and biosafety with average particle size of about 100 nm, which could selectively internalize into pancreatic cancer cells BxPC-3 during the short incubation time (1–4 h) in vitro under the mediation of CKAAKN peptide, compared with HPDE6-C7 cells. Furtherly, significantly higher uptake of ORI@CPD-Lipo nanoparticles by BxPC-3 tumors (positive-CKAAKN) than normal pancreatic tissue was observed in vivo during the whole experiment process (1–48 h), with increased ratio on experiment time. Our data also indicated the treatment of BxPC-3 cells with ORI@CPD-Lipo nanoparticles showed significantly enhanced antitumor effect and therapeutic efficacy than the treatment with the free ORI, non-targeting ORI@Lipo and CKAAKN-blocking ORI@CPD-Lipo. The enhanced antitumor activity to BxPC-3 cells should be attributed to the enhanced drug cellular uptake mediated by ORI@CPD-Lipo nanoparticles, chemical toxicity of the released ORI from the nanoparticles. Our study provided a valuable reference and strategy of CKAAKN-mediated targeting cancer chemotherapy based on ORI@CPD-Lipo against the pancreatic tumor.
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npj Computational Materials
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2026
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IF 11.9
Huang Lin,Zhang Xinyu,Li Shuxing,Xie Rongjun
The data-driven discovery of luminescent materials is hindered by a critical paradox: while decades of research have generated extensive data, the unstructured textual format of such data in literature precludes its systematic reuse. While text mining enables conversion to structured datasets, general natural language processing (NLP) tools fail at domain-specific challenges, such as fragmented host-dopant syntax and incomplete representations of property relationships. To address these limitations, we developed a novel NLP pipeline specifically tailored to the phosphor domain, integrating three key algorithms: chemically-aware parsing, frequency-driven subject recovery, and context-aware disambiguation algorithms. Using this approach, we successfully extracted 6,400 material-emission relationships from 16,659 scientific papers, with F1 scores of 0.956 for phosphor formula extraction and 0.851 for relation extraction. Additionally, a machine learning model trained on the extracted dataset predicted Eu 2+ emission wavelengths, with a coefficient of determination (R 2 ) of 0.91. Guided by predictions, we synthesized novel phosphors (e.g., CaGd 2 S 4 : Eu 2+ ), the experimental emission spectra of these materials deviated by around 10 nm from predictions, verifying the robustness of the “data extraction-model prediction-experimental validation” workflow. This study bridges computational discovery and experimental validation, offering an open framework and tools to accelerate luminescent materials innovation.
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APOPTOSIS
·
2026
·Vol. 31
·期 2
·52
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IF 8.1
Mao Dong,Sun Jufeng,Zhang Xiaowei,Wang Zhaoping,Zhang Yun
Colorectal cancer (CRC) is a malignant tumor originating from the epithelial cells of the colon and rectum. Interleukin enhancer binding factor 2 (ILF2), an emerging RNA-binding protein, has been implicated in the regulation of multiple cancer-related processes. However, its specific role in the pathogenesis and progression of CRC remains largely unexplored. In this study, we systematically investigated the functional role of ILF2 in CRC through multi-level experiments. ILF2 expression was first assessed in clinical CRC tissues via quantitative real-time PCR and western blot. Functional implications were further examined through gain-of-function and loss-of-function approaches. Cellular assays included CCK-8 proliferation, colony formation, Transwell migration/invasion, macrophage coculture, and flow cytometry for apoptosis, complemented by immunofluorescence staining. In vivo relevance was confirmed using xenograft models for tumor growth and metastasis. Mechanistically, Co-IP revealed a robust interaction between ILF2 and ILF3, while RIP, RNA pull-down and dual-luciferase reporter assays demonstrated direct binding of ILF3 to KLF16 mRNA, suggesting a novel regulatory axis in CRC progression. Our results demonstrated that both ILF2 mRNA and protein were significantly upregulated in colorectal cancer (CRC) tissues compared to matched peritumoral tissues. Functional assays revealed that ILF2 overexpression enhanced, whereas ILF2 knockdown suppressed, the proliferation, migration, and invasion capabilities of CRC cells in vitro. Additionally, ILF2 overexpression induced M0 macrophages to polarize toward an M2-like phenotype. Consistent with these findings, in vivo experiments indicated that ILF2 facilitated tumor growth and promoted liver metastasis in CRC. Our work suggests that ILF2 formed a complex with ILF3 to enhance the stability of KLF16 mRNA, thereby contributing to CRC progression through the regulation of KLF16. Graphical abstract A proposed working model of the ILF2/ILF3/KLF16/β-catenin axis in colorectal cancer progression. Hypothetical model illustrating that ILF2 binding to ILF3 stabilizes KLF16 mRNA, which in turn activates β-catenin signaling. This pathway ultimately promotes CRC cell proliferation, invasion, liver metastasis, and M2-type macrophage polarization.
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Journal of Central South University
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2026
·Vol. 32
·期 11
·4248-4259
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IF 4.4
Wang Jia-yi,Liu Xin-li,Wu Zhuang-zhi,Wang De-zhi
Eco-friendly electrocatalytic nitrogen reduction reaction (NRR) is aimed to replace the traditional polluting industrial process, but NRR needs electrocatalysts with high selectivity and activity to boost desired NH 3 yield rate and Faradic efficiency (FE). In this work, high-entropy sulfides (HES) (FeCoNiMoM)S x (M=Cr, Cu, Mn) were synthesized via a two-step solvothermal method. The optimized composition for HES is (FeCoNiMoCr)S x , with promising NRR performance that NH 3 yield rate reached 47.97 µg/(h·mg cat ) at −0.7 V vs RHE and FE was 26.1% at −0.4 V vs RHE. Comprehensive characterization and electrochemical testing were performed to investigate the effects of the metal component on NRR performance. It reveals that (FeCoNiMoCr)S x shows more intense charge transport, more electrocatalytic active sites, higher selectivity, etc, resulting from the electron transport and element synergy of HES. Also, it is proved to have targeted NRR selectivity and limiting competitive hydrogen evolution reaction. The results offer promising guidance for further improving the NRR electrocatalysts based on transition elements.
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JOURNAL OF ELECTRONIC MATERIALS
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2015
·Vol. 45
·期 3
·1290-1295
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IF 1.798
Gao Jie,Liu Chengyan,Miao Lei,Wang Xiaoyang,Chen Yu
With high carrier mobility and low cost, reduced graphene oxide (RGO) shows bright prospects for use in the field of thermoelectric materials. To investigate the intrinsic thermoelectric properties of RGO sheets, we prepared RGO papers which were reduced by HBr solution for 5 min, 20 min, and 60 min, respectively. Thermogravimetry analysis (TGA) and Raman analysis showed that the conjugated carbon network of graphene oxide (GO) was restored during the reduction process and the thermal stability of the RGO papers was much better than that of GO paper. The RGO paper that was reduced for 60 min and then annealed in Ar/H 2 atmosphere exhibited the highest electrical conductivity of 3.22 × 10 5 S/m at 160°C. As the reduction degree of the RGO paper deepens, the Seebeck coefficient gradually transforms from positive to negative, indicating that the conduction type of RGO paper can be controlled by regulating the reduction degree.
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MICROCHIMICA ACTA
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2018
·Vol. 185
·期 12
·530
·
IF 5.705
Ding Chuanmin,Song Kaijing,Meng Hongyun,Zhang Bing,Zhao Zhihuan,Chang Honghong,Wei Wenlong
The authors describe an amplified photoelectrochemical immunoassay for the tumor marker carbohydrate antigen 724 (CA724). The method employs a C 3 N 4 -MoS 2 semiconductor as the photoelectric conversion layer. The nanocomposite was characterized by transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray powder diffraction, and UV-vis diffuse reflectometry. The dye eosin Y was encapsulated into CaCO 3 nanospheres which then were used as labels for antibody against CA724. In addition, Fe 3 O 4 nanospheres were employed as magnetic platform for constructing photoelectrochemical sandwich immunoassay. The CaCO 3 nanospheres can be dissolved with aid of ethylene diamine tetraacetic acid (EDTA) and the carried eosin Y in CaCO 3 is released. The released dyes sensitizes the C 3 N 4 -MoS 2 semiconductor, which induces photocurrent amplification. Under optimal conditions and at a typical working voltage of 0 V (vs. SCE), the photocurrent increases linearly in the range of 0.05 mU mL −1 to 500 mU mL −1 of CA724, with a 0.02 mU mL −1 detection limit.
The C 3 N 4 -MoS 2 complex, with high efficiency of electron transport, was synthesized to construct a photoelectrochemical analytical platform. A sandwich-type immunoassay was established on the surface of magnetic beads. Carbohydrate antigen 724 in sample was detected sensitively by using sensitization of released eosin Y as signal amplifiery.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
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2020
·Vol. 31
·期 20
·18209-18220
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IF 2.22
Zhang Hongshuai,Meng Yanshuang,Hu Qianru,Zhao Guixiang,Zhu Fuliang,Zhang Yue
MoS 2 is a promising anode with high theoretical capacity in lithium-/sodium-ion batteries. Nevertheless, the unstable structure and poor conductivity of MoS 2 limit its practical applications. Herein, a carbon layer-coated MoS 2 composite (MoS 2 /C) is synthesized by a pyrolysis strategy, in which ionic liquid-derived carbon improves the conductivity and structural stability of composites by wrapping MoS 2 . When MoS 2 /C composite is used as anode in lithium-/sodium-ion batteries, it exhibits excellent stability, high capacity and long cycle life. After 50 cycles at 100 mA g −1 , the lithium storage capacity of the MoS 2 /C composite is more than 760 mAh g −1 , and the sodium storage capacity reaches 480 mAh g −1 . When the current density is 2 A g −1 , the sodium storage capacity maintains at 355 mAh g −1 after 500 cycles. Even after 1000 cycles, it still exhibits a stable lithium storage capacity of 350 mAh g −1 at the current density of 4 A g −1 . The improved performance of MoS 2 /C composite can be explained by its stable structure and high charge mobility.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
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2017
·Vol. 29
·期 5
·4266-4275
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IF 2.019
Zhou Tao,Fu Shenna,Ma Li,Gan Mengyu,Wang Huihui,Hao Chenxia
Metal-free 1D polyaniline nanotubes/carbon nitride (PNCN) hollow composites are fabricated by self-assembly chemisorption and solvent evaporation method for the first time. The results show that carbon nitride (g-C 3 N 4 ) is successfully covered onto polyaniline (PANI) nanotubes. Due to similar electronic structures of nitrogen-rich materials of PANI and g-C 3 N 4 , the combination of two π-conjugated system not only benefits the separation of photogenerated charge carriers but also increases the light absorption range of g-C 3 N 4 . The compact contact of the PANI nanotubes and g-C 3 N 4 nanosheets as well as their matched energy level is the determinant factor for interfacial heterojunction. Specially, the hollow 1D “Janus” heterojunction impels photogenerated charge pairs to separate and transfer to opposite directions in a short diffusion path, with the additional benefits of exposure of more active sites and transmission of reactants for catalytic surface reactions. As expected, PNCN composites are photoactive and exhibit enhanced photocatalytic performance under visible-light irradiation over pure g-C 3 N 4 and PANI nanotubes. In addition, PNCN composites possess strong adsorption capacity. This versatile 1D PNCN hollow composites prepared from a cost-effective and green process have potential applications in catalysis, drug delivery system, sensors, and energy storage and conversion.
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APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
·
2023
·Vol. 129
·期 12
·877
·
IF 2.7
Ding Feng,Qiu Guobin,Tan Fengliang,Wu Tengyan,Ding Longzhen,Liu Xin,Zhong Hongbin
Characterization results of Fourier transformed infrared spectrometer(FT-IR), thermogravimetry–differential thermal analysis TG–DTA, dynamic stability control (DSC), transmission electron microscopy (TEM), and vibrating sample magnetometer (VSM) indicated that DOX loaded onto PEGylated superparamagnetic Mn 0.6 Zn 0.4 Gd 0.04 Fe 1.96 O 4 (MNPs) was prepared successfully. DOX loading ration and release mechanism was analyzed; the result indicated that the DOX loading ration increased with initial charge DOX increasing, and DOX release was a Fickian diffusion mechanism, with 34.5% of DOX released in PBS solution at 43 °C within 168 h. Consequently, the MNPs@PEG may be a good targeted drug delivery system for tumor targeted and hyperthermia MNPs as therapeutic applications.
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