Nature Protocols
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2023
·
IF 14.8
Li Hao,Zhu Lele,Fan Ruixiao,Li Zheng,Liu Yifan,Shaheen Aaqib,Nie Fang,Li Can,Liu Xuqin,Li Yuanyuan,Liu Wenjuan,Yang Yingying,Guo Tutu,Zhu Yu,Bu Mengchen,Li Chenglin,Liang Huihui,Bai Shenglong,Ma Feifei,Guo Guanghui,Zhang Zhen,Huang Jinling,Zhou Yun,Song Chun-Peng
Breeding new and sustainable crop cultivars of high yields and desirable traits has been a major challenge for ensuring food security for the growing global human population. For polyploid crops such as wheat, introducing genetic variation from wild relatives of its subgenomes is a key strategy to improve the quality of their breeding pools. Over the past decades, considerable progress has been made in speed breeding, genome sequencing, high-throughput phenotyping and genomics-assisted breeding, which now allows us to realize whole-genome introgression from wild relatives to modern crops. Here, we present a standardized protocol to rapidly introgress the entire genome of Aegilops tauschii , the progenitor of the D subgenome of bread wheat, into elite wheat backgrounds. This protocol integrates multiple modern high-throughput technologies and includes three major phases: development of synthetic octaploid wheat, generation of hexaploid A. tauschii –wheat introgression lines (A-WIs) and homozygosis of the generated A-WIs. Our approach readily generates stable introgression lines in 2 y, thus greatly accelerating the generation of A-WIs and the introduction of desirable genes from A. tauschii to wheat cultivars. These A-WIs are valuable for wheat-breeding programs and functional gene discovery. The current protocol can be easily modified and used for introgressing the genomes of wild relatives to other polyploid crops.
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高影响因子
Nature Communications
·
2023
·Vol. 14
·期 1
·1-14
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IF 16.6
Yang Yuxuan,Zhao Xiaodan,Wang Shuang,Zhang Yanfeng,Yang Aiming,Cheng Yilong,Chen Xuesi
Osteoarthritis is a worldwide prevalent disease that imposes a significant socioeconomic burden on individuals and healthcare systems. Achieving cartilage regeneration in patients with osteoarthritis remains challenging clinically. In this work, we construct a multiple hydrogen-bond crosslinked hydrogel loaded with tannic acid and Kartogenin by polyaddition reaction as a cell-free scaffold for in vivo cartilage regeneration, which features ultra-durable mechanical properties and stage-dependent drug release behavior. We demonstrate that the hydrogel can withstand 28000 loading-unloading mechanical cycles and exhibits fast shape memory at body temperature (30 s) with the potential for minimally invasive surgery. We find that the hydrogel can also alleviate the inflammatory reaction and regulate oxidative stress in situ to establish a microenvironment conducive to healing. We show that the sequential release of tannic acid and Kartogenin can promote the migration of bone marrow mesenchymal stem cells into the hydrogel scaffold, followed by the induction of chondrocyte differentiation, thus leading to full-thickness cartilage regeneration in vivo. This work may provide a promising solution to address the problem of cartilage regeneration.
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Chemical and Biological Technologies in Agriculture
·
2023
·Vol. 10
·期 1
·1-16
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IF 6.6
Zhong Ximing,Lan Ruopeng,Su Guofeng,Hao Li,Xu Hua,Zhou Hongjun,Zhou Xinhua
Background The growing escalation of soil salinization is tremendously threatening the global food security and the development of sustainable agriculture. To address the worldwide predicament caused by salt stress toward crops, combining nanotechnology with the merits of plant hormone may become an efficient and effective approach. Results In this work, a sustained release agent system (BR@MSN) was developed by loading brassinolide (BR) to mesoporous silica nanoparticles (MSN) to enhance the salt stress resistance of cucumber seeds and seedlings. The obtained BR@MSN agent was about 120 nm. As an endogenous plant hormone, promotion in crop growth was found at low BR concentration. Due to the sustained release property, BR@MSN avoided excessive BR exposure to seeds and seedlings to cause inhibitory effects. After the soil application of BR@MSN, the promotion effect from BR combined with the regulation enhancement from MSN nanocarrier improved the seed germination rate by 11.76% under saline environment. Compared with the same BR concentration (2.0 mg/L), BR@MSN increased the seed germination rate even by 1324.29%. In addition, remarkable wettability on foliar surfaces was found, and the foliar application of BR@MSN significantly enhanced the salt stress resistance of cucumber seedlings by alleviating the accumulation of reactive oxygen species (ROS) and increasing the cell viability along with the improvement in superoxide dismutase (SOD) activity (234.11%), the decrease in malondialdehyde (MDA) content (61.30%), and the increase in chlorophyll content (110.88%). Conclusions The newly developed BR@MSN agents could effectively enhance the salt stress resistance of crop seeds and seedlings, and their applications significantly improved the seed germination rate and seedling growth. The remarkable efficacy makes this BR@MSN agent system potential in agricultural field for enhancing the salt stress resistance of crops and facilitating the development of sustainable agriculture. Graphical Abstract
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JOURNAL OF POLYMER RESEARCH
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2023
·Vol. 30
·期 12
·1-12
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IF 2.8
Huang Baoli,Ma Meng,Wang Yueyin,Chen Si,Shi Yanqin,He Huiwen,Wang Xu
Rational design morphology of flame retardant is crucial to achieving a balance between satisfactory flame retardancy and superior mechanical properties. However, there are few studies focused on the morphology of flame retardants, and how to achieve the aim of satisfactory flame retardancy yet low mechanical properties loss is still a challenge. Herein, aluminum diethylphosphinate ( ADP) with different morphologies were obtained through hydrothermal synthesis method via adjusting the releasing rate of Al 3+ , molar ratio (OH − : Al 3+ ), and changing the type of acid. The morphology of ADP was successfully regulated from random (ADP-1), rod-like (ADP-2) to flower-like (ADP-3). The formation mechanism of different morphology was discussed in detail. The TG results indicated that all ADP have high thermal stability. Therefore, ADP as flame retardant was used for modification of TPU. Compared to neat TPU, TPU/ADP composites present lower initial decomposition temperature and higher char residual. With addition of 30% ADP-2 the LOI value increased from 22.5 to 28.0%, achieved the UL-94 V-2 rating, respectively. THR was 25.6% lower than that of virgin TPU, exhibiting higher fire safety performance. Meanwhile the tensile strength of TPU composite with ADP-2 (10.8 ± 0.4) is higher than that of the composites with ADP-1 (8.7 ± 0.9), and ADP-3 (7.9 ± 0.5) at content up to 30%. This work provides a promising strategy to tune the morphology of flame retardant and compromise the contradiction of poor mechanical properties with high fire safety of polymer composite.
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Nature Communications
·
2023
·Vol. 14
·期 1
·1-10
·
IF 16.6
Ai Liqing,Lin Weikang,Cao Chunyan,Li Pengyu,Wang Xuejiao,Lv Dong,Li Xin,Yang Zhengbao,Yao Xi
The rapid-developing soft robots and wearable devices require flexible conductive materials to maintain electric functions over a large range of deformations. Considerable efforts are made to develop stretchable conductive materials; little attention is paid to the frequent failures of integrated circuits caused by the interface mismatch of soft substrates and rigid silicon-based microelectronics. Here, we present a stretchable solder with good weldability that can strongly bond with electronic components, benefiting from the hierarchical assemblies of liquid metal particles, small-molecule modulators, and non-covalently crosslinked polymer matrix. Our self-solder shows high conductivity (>2×10 5 S m −1 ), extreme stretchability (~1000%, and >600% with chip-integrated), and high toughness (~20 MJ m −3 ). Additionally, the dynamic interactions within our solder’s surface and interior enable a range of unique features, including ease of integration, component substitution, and circuit recyclability. With all these features, we demonstrated an application as thermoforming technology for three-dimensional (3D) conformable electronics, showing potential in reducing the complexity of microchip interfacing, as well as scalable fabrication of chip-integrated stretchable circuits and 3D electronics.
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CHEMICAL PAPERS
·
2023
·
IF 2.2
Hong Ye,Yu Kanping
Cotton-based clothing fabrics are welcome because they are warm, close-fitting, hygroscopic and breathable. However, surface hydrophilic hydroxyls will make them easy to stick to stains. High-cost preparing of inorganic nanoparticles can favor a building of hydrophobic coatings for them. To cheaply attain surface self-cleaning attributes of cotton-based fabrics, here, all-organic surface coatings were established utilizing surface-fluorinated uninjured polyglycidyl methacrylate (PGMA)/polyvinylidene fluoride (PVDF) co-modifying. High PVDF dosage incurred a large water contact angle (WCA) of treated fabric, and high hydrophilic attribute of pristine fabric was altered into high hydrophobic one. Antifouling attribute was acquired on the surfaces of treated fabrics, and effective ultraviolet (UV) and wearing resistances of the optimal coating/fabric composite with feeding 10 wt% of PVDF were fulfilled. The optimal fabric surface demonstrates a superhydrophobic attribute (WCA at 153.5 ± 1.5°). After UV irradiating for 24 h or surface abrasion for 100 cycles, this surface demonstrates a WCA surpassing 140° with a water sliding angle below 16°. The fiber weaving constitution and surface F assembling are critical for performance optimizing. The current research may give an effective route for large-area producing of self-cleaning cotton-based fabrics.
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POLYMER BULLETIN
·
2023
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IF 3.2
Chen Zhengpeng,Li Jie,Guo Hongyun,Zhang Yongdong,Ma Mingzhong,Zhou Xing,Yao Wenhuan,Ma Hengchang,Lei Ziqiang,Lu Dedai
Transcatheter arterial embolization has been used clinically for the treatment of intermediate to advanced unresectable tumors, which is a complex procedure and may pose other risks. In order to achieve an efficient and simple therapeutic diagnostic method for tumors, this paper is based on the fact that the fluorescence properties of AIE fluorescent molecules are similar to those of pH-responsive polyamino acid materials undergoing a “sol–gel” phase transition in a tumor micro-acid environment, and two polyamino acid materials, PGTTC and PGCLTGCL, were linked with TPA derivatives of AIE fluorescent molecules to obtain catheter-free embolic agents, PGTTC-DCDPP-2TPA and PGCLTGCL-DCDPP-2TPA. In tumor fluorescence imaging experiments in tumor-bearing mice, both were enriched at the tumor 72 h after injection and that of the PGCLTGCL-DCDPP-2TPA was stronger, moreover, the tumor cells were suppressed and necrotic after 25 days. Therefore, this pH-responsive noninterventional embolization combination therapeutic diagnostic system has good prospects for application in the field of combined therapeutic diagnostic multifunctional platforms.
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Nanoscale Research Letters
·
2018
·Vol. 13
·期 1
·1-12
·
IF 3.125
Zhang Xueyan,Wu Fengbo,Men Ke,Huang Rong,Zhou Bailin,Zhang Rui,Zou Rui,Yang Li
As a novel toll-like receptor 9 (TLR9) agonist, synthetic unmethylated cytosine-phosphate-guanine (CpG) oligodeoxynucleotides can stimulate a Th1 immune response and potentially be used as therapeutic agents or vaccine adjuvants for the treatment of cancer. However, some drawbacks of CpG limit their applications, such as rapid elimination by nuclease-mediated degradation and poor cellular uptake. Therefore, repeat high-dose drug administration is required for treatment. In this work, a CpG delivery system based on 3-aminopropyltriethoxysilane (APTES)-modified Fe 3 O 4 nanoparticles (FeNPs) was designed and studied for the first time to achieve better bioactivity of CpG. In our results, we designed FeNP-delivered CpG particles (FeNP/CpG) with a small average size of approximately 50 nm by loading CpG into FeNPs. The FeNP/CpG particle delivery system, with enhanced cell uptake of CpG in bone marrow-derived dendritic cells (BMDCs) in vitro and through intratumoral injection, showed significant antitumor ability by stimulating better humoral and cellular immune responses in C26 colon cancer and 4T1 breast cancer xenograft models in vivo over those of free CpG. Moreover, mice treated by FeNP/CpG particles had delayed tumor growth with an inhibitory rate as high as 94.4%. In addition, approximately 50% of the tumors in the C26 model appeared to regress completely. Similarly, there were lower pulmonary metastases and a 69% tumor inhibitory rate in the 4T1 breast cancer tumor model than those in the untreated controls. In addition to their effectiveness, the easy preparation, safety, and high stability of FeNP/CpG particles also make them an attractive antitumor immunotherapy.
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BIOCHEMICAL GENETICS
·
2024
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IF 2.1
Wang Song,Bao Ning,Li Mohan,Liu Dongwei,Tao Liming
Proliferative diabetic retinopathy (PDR), the most common type of diabetic retinopathy, is a main cause of visual and impairment blindness. Abnormal neovascularization, endothelial dysfunction, and vascular inflammation are important mechanisms for the development of PDR. Ets2 regulates angiogenesis-related genes and inflammation, however, the effect of Ets2 in PDR procession has not been clarified. Thus, this study is performed to investigate whether Ets2 exerts key functions in PDR. In this study, 10-week-old mice were used for establishing STZ-induced diabetic mice, and Ets2 expression was analyzed in retina tissues. Besides, newborn mice were applied to construct oxygen-induced retinopathy (OIR) models. The Ets2 expression, oxidative stress, and inflammation were detected in retina tissues. We found that Ets2 was highly expressed in retina tissues both in diabetic mice and OIR mice. Oxidative stress and inflammatory processes are two factors contributing to the pathogenesis of PDR. In retinal tissues of OIR mice, Ets2 knockdown inhibited expression of inflammatory mediators VEGFA, IL-6, and IL-8, and biomarkers of oxidative stress MCP-1, VCAM-1, and iNOS. ROS production was also inhibited by silencing Ets2. Ets2 deficiency inhibited endothelial cell proliferation in the retina. Furthermore, Ets2 knockdown contributed to suppressing the expression of angiogenesis-related genes VEGFA, JUNB, MMP-9, Tie2, Ang-2, and EphB4. Our study highlights that Ets2 accelerates PDR procession by promoting the proliferation of endothelial cells, oxidative stress, and inflammation, which provides a novel target against PDR.
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Nature Communications
·
2021
·Vol. 12
·期 1
·1-9
·
IF 14.919
Wang Xu-Dong,Huang Yu-Hua,Liao Jin-Feng,Wei Ze-Feng,Li Wen-Guang,Xu Yang-Fan,Chen Hong-Yan,Kuang Dai-Bin
Halide perovskite single-crystals have recently been widely highlighted to possess high light harvesting capability and superior charge transport behaviour, which further enable their attractive performance in photovoltaics. However, their application in photoelectrochemical cells has not yet been reported. Here, a methylammonium lead bromide MAPbBr 3 single-crystal thin film is reported as a photoanode with potential application in photoelectrochemical organic synthesis, 2,5-dimethoxy-2,5-dihydrofuran. Depositing an ultrathin Al 2 O 3 layer is found to effectively passivate perovskite surface defects. Thus, the nearly 5-fold increase in photoelectrochemical performance with the saturated current being increased from 1.2 to 5.5 mA cm −2 is mainly attributed to suppressed trap-assisted recombination for MAPbBr 3 single-crystal thin film/Al 2 O 3 . In addition, Ti 3+ -species-rich titanium deposition has been introduced not only as a protective film but also as a catalytic layer to further advance performance and stability. As an encouraging result, the photoelectrochemical performance and stability of MAPbBr 3 single-crystal thin film/Al 2 O 3 /Ti-based photoanode have been significantly improved for 6 h continuous dimethoxydihydrofuran evolution test with a high Faraday efficiency of 93%.
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Nature Communications
·
2023
·Vol. 14
·期 1
·1-13
·
IF 16.6
Wang Xingkun,Xu Liangliang,Li Cheng,Zhang Canhui,Yao Hanxu,Xu Ren,Cui Peixin,Zheng Xusheng,Gu Meng,Lee Jinwoo,Jiang Heqing,Huang Minghua
Dual atom catalysts, bridging single atom and metal/alloy nanoparticle catalysts, offer more opportunities to enhance the kinetics and multifunctional performance of oxygen reduction/evolution and hydrogen evolution reactions. However, the rational design of efficient multifunctional dual atom catalysts remains a blind area and is challenging. In this study, we achieved controllable regulation from Co nanoparticles to CoN 4 single atoms to Co 2 N 5 dual atoms using an atomization and sintering strategy via an N-stripping and thermal-migrating process. More importantly, this strategy could be extended to the fabrication of 22 distinct dual atom catalysts. In particular, the Co 2 N 5 dual atom with tailored spin states could achieve ideally balanced adsorption/desorption of intermediates, thus realizing superior multifunctional activity. In addition, it endows Zn-air batteries with long-term stability for 800 h, allows water splitting to continuously operate for 1000 h, and can enable solar-powered water splitting systems with uninterrupted large-scale hydrogen production throughout day and night. This universal and scalable strategy provides opportunities for the controlled design of efficient multifunctional dual atom catalysts in energy conversion technologies.
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Advanced Fiber Materials
·
2023
·
IF 16.1
Cui Yifan,He Xinyang,Liu Wendi,Zhu Suiyuan,Zhou Man,Wang Qiang
Stretchable thermoelectric-based self-powered sensors have attracted widespread attention for wearable electronic devices. However, the development of thermoelectric materials with wearable comfort, green, and multimodal synergy remains challenging. In this paper, we prepared a poly(3,4-ethylenedioxythiophene)/multi-walled carbon nanotube (PEDOT/MWCNT)-based thermoelectric fabric for self-powered strain–temperature dual-parameter sensing via spraying and in situ bio-polymerization. Compared with ferric chloride (FeCl 3 ), used in chemical polymerization, the PEDOT thermoelectric fabric prepared by enzymatic polymerization is not doped with metal ions, making the thermoelectric performance of flexible wearable fabrics more stable. In addition, the energy-filtration effect of PEDOT and MWCNT efficiently enhanced the thermoelectric performance of the fabric. The fabric has over 320% elongation potential and excellent breathability while exhibiting excellent wearability. Moreover, the fabric-based sensor had a wide strain range (1–100%) and temperature detection limit (1 °C). In addition, fabric-based sensors were tested by sewing them directly onto clothing or attachment accessories, and showed a rapid response to changes in human joint bending and microenvironmental temperature differences. Moreover, the sensor could be integrated into an intelligent firefighting suit, to continuously and synergistically monitor health abnormalities in firefighter's body movement and temperature thresholds in the micro-environmental temperature of the suit. The developed self-powered dual-parameter wearable sensor shows fascinating potential for applications in human health monitoring, human–computer interaction devices, and intelligent robotics. Graphical abstract
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危险品化学品经营许可证（带存储）