FOOD CHEMISTRY
·
2020
·
IF 6.306
Jiaxin Wang,Xuening Lu,Jiaen Zhang,Zeheng Xiao
Lipids such as phosphatidylcholine (PC), 3- sn -phosphatidylethanolamine (PE), sphingomyelin (SM) and L-α-lysophosphatidylcholine (LPC) are the major components of biological membranes and play important roles in physiological functions. Here, PC, PE, SM, and LPC were extracted from golden apple snails (GAS, Pomacea canaliculata ) and GAS flesh (GASF) using an ethanol/hexane sequential scheme and quantified simultaneously using ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UHPLC-ESI-MS/MS) to evaluate whether the GAS could be the source of the four lipids. Our results suggest that ethanol extracts contained the most crude lipids, and the yield of dry (evaporated) lipids were 3.45 g per 100 g fresh GASF and 1.82 g per 100 g of fresh GAS. Quantification of the lipids using UHPLC-ESI-MS/MS suggested that GAS contained PE, PC, SM and LPC, with SM being the most abundant lipid (after purification: 1.71 and 1.42 mg g −1 dry weight from 100 g of GASF and GAS, respectively). The method we used is cost-effective, and the recovery rates of ethanol and hexane ranged from 80−91% and 87−91% respectively. Overall, GAS and GASF are potential raw materials for lipids such as SM and PC extraction using the ethanol/hexane method. Comparatively, lipids extraction from the GAS is more effective and timesaving. Our finding would provide a way to utilize GAS and potentially control its invasion.
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Materials Today Bio
·
2025
·
IF 10.2
Shuangying Yang,Youjia Wu,Xiaoyuan Huang,Xiaoyu Li,Yao Wang,Jianfeng Pan,Zhengjun Huang,Shaohuang Weng,Jianyong Huang
The treatment of infectious wounds is confronted with the challenges of antibiotic-resistant bacterial infections and excessive inflammatory responses, significantly impacting the healing process. A multifunctional hydrogel dressing system used as local strategy is needed to address this clinical dilemma. Herein, a kind of carbon dots (P-CDs) were synthesized using polyhexamethylene guanidine (PHMG) and citric acid (CA) through a hydrothermal method, exhibiting excellent broad-spectrum antibacterial activity, including effective inhibition of clinically isolated resistant bacteria. The antibacterial mechanism of P-CDs involved the generation of reactive oxygen species, inhibition of ATP synthesis, and disruption of bacterial membrane integrity with the leakage of protein. Additionally, P-CDs demonstrate pronounced immunomodulatory properties by inducing macrophage polarization toward the M2 phenotype, thus modulating the inflammatory microenvironment. These functional components were incorporated into an intelligent hydrogel system (P-CDs@EPCS) constructed from ε-poly-L-lysine and carboxymethyl chitosan, resulting in a wound dressing with tissue adhesion, resilient maintenance of mechanical integrity, and favorable mechanical properties. P-CDs@EPCS exhibited a sustained pH-responsive drug release profile for up to 10 days, maintaining the stability of active constituents throughout the release period. In vivo experiments confirmed that P-CDs@EPCS significantly accelerated wound healing in infections caused by Pseudomonas aeruginosa , demonstrating a synergistic effect of antibacterial, anti-inflammatory, and repair-promoting actions. This study provides a promising solution for treating infectious wounds, integrating key advancements in the field of hydrogels, including dynamic crosslinking design and smart drug delivery systems.
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PLANT CELL AND ENVIRONMENT
·
2024
·Vol. 47
·期 3
·913-927
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IF 6
Guanhua Liu,Qian Wang,Hui Chen,Yuxi Wang,Xiaogui Zhou,Demeng Bao,Nuo Wang,Juan Sun,Fuyin Huang,Mei Yang,Han Zhang,Peng Yan,Xin Li,Jiang Shi,Jianyu Fu
Insect-induced plant volatile organic compounds (VOCs) may function as either direct defence molecules to deter insects or indirect defence signals to attract the natural enemies of the invading insects. Tea ( Camellia sinensis L.), an important leaf-based beverage crop, is mainly infested by Ectropis obliqua which causes the most serious damage. Here, we report a mechanistic investigation of tea plant-derived VOCs in an indirect defence mechanism against E. obliqua . Parasitoid wasp Parapanteles hyposidrae , a natural enemy of E. obliqua , showed strong electrophysiological response and selection behaviour towards S -linalool and β-ocimene, two monoterpenes with elevated emission from E. obliqua -damaged tea plants. Larvae frass of E. obliqua , which also released S -linalool and β-ocimene, was found to attract both mated female or male Pa. hyposidrae according to gas chromatography-electroantennogram detection and Y-tube olfactometer assays. In a field setting, both S -linalool and β-ocimene were effective in recruiting both female and male Pa. hyposidrae wasps. To understand the molecular mechanism of monoterpenes-mediated indirect defence in tea plants, two novel monoterpene synthase genes, CsLIS and CsOCS-SCZ , involved in the biosynthesis of S -linalool or β-ocimene, respectively, were identified and biochemically characterised. When the expression of these two genes in tea plants was inhibited by antisense oligodeoxynucleotide, both volatile emission and attraction of wasps were reduced. Furthermore, gene expression analysis suggested that the expression of CsLIS and CsOCS-SCZ is regulated by the jasmonic acid signalling pathway in the tea plant.
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CHROMATOGRAPHIA
·
2026
·
IF 1.3
Yang Weiqi,Li Xiaofei,Wang Wenhao,Huang Qi,Fan Hui,Qian Zhengming
Bistorta vivipara L. achenes ( B. vivipara achenes), rich in phenolic acids and flavonoids, are a valuable food and medicinal resource. However, a detailed analysis of its individual components and their mechanisms remains unexplored. This study introduces solid-phase extraction (SPE) for selectively isolating and purifying total phenolic acids and flavonoids from B. vivipara achenes. The antioxidant potential of the extracts was evaluated using ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), DPPH (2,2-diphenyl-1-picrylhydrazyl), and FRAP (ferric ion reducing antioxidant potential) assays. Results showed significant antioxidant activity in the total phenolic acid extract (125.1 ± 0.7 mg GAE/g dry weight [DW]) and total flavonoid extract (1491.1 ± 19.9 mg RE/g DW). Using online FRAP–high-performance liquid chromatography (FRAP–HPLC), seven antioxidant components were identified in the phenolic acid fraction and thirteen in the flavonoid fraction. Notably, protocatechuic acid, cryptochlorogenic acid, isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C, shaftoside, isoshaftoside, isoorientin, taxifolin, and vitexin are reported for the first time as antioxidant constituents in B. vivipara achenes. These findings highlight B. vivipara achenes as a promising source of natural antioxidants and functional food ingredients. The SPE–FRAP–HPLC method enables comprehensive analysis by selectively enriching active compounds and enhancing sensitivity and accuracy compared with crude extracts. Furthermore, the application of online FRAP–HPLC facilitates real-time monitoring of the antioxidant activity of individual compounds during chromatographic separation, providing a detailed antioxidant activity profile of phenolic compounds in B. vivipara achenes. Graphical
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MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Meiling Long,Yue Liu,Jun Yang,Shaogui Wu
Biomass-derived B-CDs were synthesized from Mahonia fortunei fruits. • The B-CDs showed bright blue emission with a quantum yield of 31.1%. • Pb 2+ selectively quenched B-CD fluorescence with a low LOD of 26 nM. • L-Histidine restored fluorescence via competitive Pb 2+ coordination. • An IMPLICATION logic gate was constructed from the on–off–on response. Nitrogen-doped carbon dots (B-CDs) with deep-blue fluorescence were synthesized through a one-step hydrothermal route using the fruits of the medicinal plant Mahonia fortunei as the biomass carbon source and 1-naphthylamine as the nitrogen dopant. The resulting B-CDs showed excitation-independent emission, a high fluorescence quantum yield of 31.1%, and excellent resistance to salt, photobleaching, and long-term storage. Pb 2+ efficiently quenched B-CD fluorescence by forming non-emissive ground-state complexes through bidentate coordination with oxygen- and nitrogen-containing surface groups, switching the probe to the “off” state. By contrast, L-histidine (L-His), whose imidazole group has stronger competitive affinity for Pb 2+ , dissociated the carbon dot‑lead complexes and markedly restored fluorescence, returning the system to the “on” state. The platform afforded a detection limit of 26 nM for Pb 2+ , while the L-His-induced fluorescence recovery response was linear over 40–80 μM, with an average fluorescence recovery efficiency of 88.2%. On the basis of this reversible fluorescence response, an IMPLICATION molecular logic gate was constructed using Pb 2+ and L-His as chemical inputs and fluorescence intensity as the output, enabling logic discrimination in a two-component system. UV–vis absorption spectroscopy, time-resolved fluorescence lifetime analysis, and FT-IR spectroscopy jointly supported the reversible “on-off-on” response mechanism. This work provides a practical strategy for developing natural plant-derived carbon-dot platforms for reversible fluorescence sensing and molecular logic devices. Download: Download high-res image (155KB) Download: Download full-size image
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Frontiers in Pharmacology
·
2026
·
IF 5.4
Jie Weng,Songlian Liu,Yajun Tong,Kewei Tang,Leilan Yin,Site Bai,Ludi Ou,Qinghua Yin,Qiang Zhou
Introduction Cervical cancer ranks as the fourth most common malignancy among women worldwide. It is closely associated with the dysregulation of numerous genes and signaling pathways. Conventional treatments for cervical cancer often lead to adverse side effects and the development of drug resistance. Methods In this study, network pharmacology was employed to identify the active components and potential targets of Crocus sativus L. Molecular docking and surface plasmon resonance analyses were used to validate the interaction between lauric acid and a key target. Lauric acid‐modified gold nanoparticles (Au@LA) were synthesized and characterized. Results Twenty‐three active components and 819 potential targets of Crocus sativus L. were identified, with PTGS2 being the main target. Au@LA selectively inhibited HeLa cell proliferation, induced apoptosis, downregulated SLC7A11 and GPX4, and modulated oxidative stress markers. In macrophages, Au@LA shifted M2 polarization to the pro‐inflammatory M1 phenotype and modulated the PGE2/EP2/cAMP‐PKA signaling axis. Conclusion Our findings indicate that Au@LA has dual anti‐cervical cancer properties through inducing ferroptosis in HeLa cells and reprogramming macrophage polarization. It shows promise as a candidate for natural product‐based nanomedicine in cervical cancer therapy, potentially opening new avenues for more effective and targeted treatments in the future.
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Antioxidants
·
2026
·Vol. 15
·期 5
·564
·
IF 8.2
Wenkai Guo,Guodong Zhuang,Jin Zhang,Huaming He,Yan Jia,Yongsheng Chen
Background/Objectives: Fingered citron ( Citrus medica L. var. sarcodactylis (Siebold ex Hoola van Nooten) Swingle) is rich in phenolic constituents, yet systematic comparisons of free and bound phenolics across tissues and origins remain limited. This study compared the peel, pulp, and blend (whole fruit) of fingered citron from five Chinese regions: Zhejiang (ZJ), Yunnan (YN), Sichuan (SC), Guangdong (GD), and Guangxi (GX). Methods: Phenolic compositions were determined by colorimetric assays and HPLC. Antioxidant activity was assessed by ORAC and PSC, and hypoglycemic-related activity by α-glucosidase and α-amylase inhibition and glucose consumption in an insulin-resistant HepG2 (IR-HepG2) cell model. Results: Phenolic distribution followed the order peel > blend > pulp, and free > bound. HPLC identified 11 free and 5 bound phenolics, predominantly hesperidin, quercetin, and 5,7-dimethoxycoumarin. GX peel exhibited the highest free phenolic content (106.34 ± 0.23 mg GAE/100 g FW) and superior ORAC (30.56 ± 0.50 μmol TE/g FW), strongly correlating with total phenolics ( r = 0.98, p < 0.01). Free phenolics showed stronger α-glucosidase and α-amylase inhibition, whereas bound phenolics produced higher glucose consumption in the IR-HepG2 cell model. The GX blend bound fraction showed the highest glucose consumption (5.48 ± 0.98 mmol/L). Conclusions: Under fresh-weight-based conditions, phenolic composition and in vitro bioactivities differed by fruit part, region, and phenolic fraction. Peel, especially GX peel, tended to show higher phenolic levels and stronger antioxidant-related performance, whereas the GX blend bound fraction showed the highest glucose-consumption-promoting activity.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2022
·
IF 5.895
Fanghua Guo,Rong Tsao,Chuyao Li,Xiaoya Wang,Hua Zhang,Li Jiang,Yong Sun,Hua Xiong
Green pea hulls are a byproduct of the processing of green pea and are rich in phenolic substances. In the present study, in vitro digestion, human colonic adenocarcinoma cell line (Caco-2) monolayer, and the Caco-2/macrophage cell lines of the murine origin (Raw264.7) coculture model were established to investigate the release of polyphenols, absorption, and transport of digestive products and their effects on inflammation and intestinal barrier. During the digestive process, polyphenols were constantly released from the pea hulls, reaching the maximum amount in the small intestine (total phenolic content (TPC): 5.41 ± 0.04 mg gallic acid (GAE)/g dry weight (DW)), and the digestive products (800 μg/mL) could reduce the secretion of NO (50.9%), IL-6 (50.6%), and TNF-α (24.6%) and inhibit the mRNA expression of cyclooxygenase-2 (COX-2) (37.2%) and inducible nitric oxide synthase (iNOS) (91.1%) compared with the lipopolysaccharide (LPS) group. A total of 12 phenolic components were quantified by ultraperformance liquid chromatography-linear ion trap orbitrap tandem mass spectrometry (UHPLC-LTQ-OrbiTrap-MS) technology. Kaempferol trihexoside in digestive products could be absorbed and transported (1.25 ± 0.13 ng quercetin/mL). The digestive products could promote the expression of claudin-1 (210.8%), occludin (64.9%), and zonulin occludin-1 (ZO-1) (52.0%) compared with the LPS group and exert anti-inflammatory effects after being absorbed. The results indicated that pea hull polyphenols could be continuously released and absorbed to play a positive role in protecting the intestinal barrier and anti-inflammatory activity.
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CHROMATOGRAPHIA
·
2022
·Vol. 85
·期 9
·809-816
·
IF 2.213
Liu Yue,Lin Yuzi,Li Ping,Yang Huiwen,Huang Qingsong,Pan Yufang
Increasing numbers of studies suggest that short-chain fatty acids (SCFAs) are closely related to diabetes. However, the complexity of fecal pretreatment makes the detection of SCFAs in feces relatively difficult. In this study, we established a sample pretreatment protocol that involved no tedious steps, requiring only the grinding of appropriate amounts of feces into powder in liquid nitrogen. The SCFA content in feces was then detected using the headspace-gas chromatography (HS-GC) method. The method achieved good linearity ( R 2 ≥ 0.9940), and the limits of quantification (LOQs) and detection (LODs) of all investigated SCFAs ranged from 0.15 to 0.45 mM and 0.05 to 0.15 mM, respectively. The recoveries of the SCFAs ranged from 91.55 to 103.96% in mouse fecal samples. The HS-GC method was then applied to evaluate the effects of a neutral polysaccharide from Acanthopanax trifoliatus (ATP1-1) on the fecal SCFA content in streptozotocin (STZ)-induced diabetic mice. After 6 weeks of treatment with 140 mg/kg/d ATP1-1, the expression levels of acetic acid, propionic acid, butyric acid, isobutyric acid, isovaleric acid and valeric acid were increased by 215.4, 88.8, 95.3, 300.8, 260.7 and 158.0%, respectively, which indicated that the hypoglycemic effect of ATP1-1 may be related to its impact on SCFAs. Our simplified pretreatment of fecal samples is suitable for the analysis and detection of SCFAs in mouse feces by HS-GC.
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JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
·
2017
·
IF 1.9
Li Xu,Caifeng Wan,Jing Du,Hongli Li,Xuesong Liu,Hong Yang,Fenghua Li
Breast cancer is the leading cause of cancer-related deaths in women and earlier detection can substantially reduce deaths from breast cancer. Polymers with targeted ligands are widely used in the field of molecular ultrasound imaging and targeted tumor therapy. In our study, the nanotheranostic agent was fabricated through filling perfluoropropane (C3F8) into poly(d,l-lactic-co-glycolic acid) nanoparticles (PLGA NPs), followed by the formation of gold nanoshell on the surface, then conjugated with anti p53 antibody which has high specificity with the p53 protein overexpressing in breast cancer. The average diameter of the gold nanoshelled PLGA NPs carrying anti p53 antibody (p53-PLGA@Au NPs) was 247 ± 108.2 nm. The p53-PLGA@Au NPs had well-defined spherical morphology and hollow interiors observed by electron microscope, and had a good photothermal effect under the irradiation of an 808 nm laser. The results of laser scanning confocal microscope (LSCM) and flow cytometer (FCM) indicated the specific targeting of p53-PLGA@Au NPs conjugating with breast cancer MCF-7 cells overexpressing p53 protein in vitro. Also the ultrasound imaging experiments in vitro showed that p53-PLGA@Au NPs were suitable for ultrasound contrast imaging. In conclusion, the p53-PLGA@Au NPs are demonstrated to be novel targeted UCAs and may have potential applications in the early diagnosis and targeted near-infrared (NIR) photothermal therapy of breast cancer in the future.
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Antioxidants
·
2022
·Vol. 11
·期 2
·332
·
IF 7.675
Hamdoon A. Mohammed,Kamal A. Qureshi,Hussein M. Ali,Mohsen S. Al-Omar,Omar Khan,Salman A. A. Mohammed
Artemisia judaica(ArJ) is a Mediterranean aromatic plant used traditionally to treat gastrointestinal ailments, skin diseases, atherosclerosis, and as an immuno-stimulant. This study describes ArJ essential oil constituents and investigates their wound healing activity. The in vitro antioxidant and antibiofilm activities of ArJ essential oil were investigated. The in vivo pro/anti-inflammatory and oxidative/antioxidant markers were compared with standard silver sulfadiazine (SS) in a second-degree skin burn experimental rat model. The gas chromatography-equipped flame ionization detector (GC-FID) analysis of ArJ essential oil revealed the major classes of compounds as oxygenated monoterpenes (>57%) and cinnamic acid derivatives (18.03%). The antimicrobial tests of ArJ essential oil revealed thatBacillus cereus,Candida albicans, andAspergillus nigerwere the most susceptible test organisms. Two second-degree burns (each 1 inch square in diameter) were created on the dorsum of rats using an aluminum cylinder heated to 120 °C for 10 s. The wounds were treated either with ArJ or SS ointments for 21 days, while the negative control remained untreated, and biopsies were obtained for histological and biochemical analysis. The ArJ group demonstrated a significant increase in antioxidant superoxide dismutase (SOD) and catalase (CAT) enzymatic activities, while lipid peroxide (LP) levels remained insignificant compared to the negative control group. Additionally, ArJ and SS groups demonstrated a significant decrease in inflammatory levels of tumor necrosis factor α (TNF-α) compared to the negative group, while interleukin 1 beta (IL-1b) and IL-6 were comparable to the negative group. At the same time, anti-inflammatory IL-10 and transforming growth factor beta 1 (TGF-b1) markers increased significantly in the ArJ group compared to the negative control. The ArJ results demonstrated potent wound healing effects, comparable to SS, attributable to antioxidant and anti-inflammatory effects as well as a high proportion of oxygenated monoterpenes and cinnamate derivatives.Keywords:Artemisia judaica;essential oil;wound healing;inflammatory markers;antibiofilm activity;antioxidant activity
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Antioxidants
·
2025
·Vol. 14
·期 9
·1065
·
IF 6.6
Fang He,Shenglan Su,Ruihan Song,Yan Li,Luyan Zou,Zongjun Li,Yu Xiao,Aixiang Hou,Ke Li,Yuanliang Wang
Although mulberry leaf (Morus albaL., ML) andSiraitia grosvenorii(SG) individually demonstrate anti-diabetic properties, their combined efficacy against type 2 diabetes mellitus (T2DM) remains unexplored. This study systematically explored the multi-target mechanisms and synergistic potential of the MLSG combination (MLSG) for T2DM intervention. We evaluated the in vitro inhibitory activities of MLSG, ML, and SG on α-amylase and α-glucosidase, alongside antioxidant capacity assessments through DPPH/ABTS radical scavenging, reducing power, and FRAP assays. Bioactive metabolites were identified using non-targeted metabolomics, while core targets and pathways were predicted using network pharmacology and validated through molecular docking. The results reveal MLSG’s significantly enhanced inhibition of α-amylase (IC50= 14.06 mg/mL) and α-glucosidase (IC50= 0.02 mg/mL) compared to individual extracts, exhibiting 1.3–15.5-fold higher potency with synergistic effects (combination index < 1). MLSG also showed improved antioxidant capacity, outperforming SG in DPPH/ABTS+scavenging and reducing power (p< 0.05), and surpassing ML in ABTS+scavenging, reducing power, and FRAP values (p< 0.05). Metabolomics identified 26 MLSG-derived metabolites with anti-T2DM potential, and network analysis pinpointed 26 active components primarily targeting STAT3, AKT1, PIK3CA, EGFR, and MAPK1 to regulate T2DM pathways. Molecular docking confirmed strong binding affinities between these components and core targets. Collectively, MLSG exerts potent synergistic anti-T2DM effects through dual-enzyme inhibition, elevated antioxidant activity, and multi-target pathway regulation, providing a solid foundation for developing MLSG as functional food ingredients.
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