Cell Communication and Signaling
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2022
·Vol. 20
·期 1
·1-11
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IF 7.525
Yang Jing,Tang Xujiao,Li Baiqiang,Shi Jinsong
In acute pancreatitis, activation of inflammatory signaling, including the nuclear factor-kappa B (NF-κB) pathway, within acinar cells is known to be an early intracellular event occurring in parallel with pathologic trypsinogen activation. Sphingosine 1-phosphate receptor 2 (S1PR2) plays a critical role in endothelial inflammation, and our previous studies reported that S1PR2 deficiency significantly reduced the inflammatory response in liver injury under cholestasis conditions. However, the role of S1PR2 in inflammatory signaling activation within acinar cells and inflammatory responses during acute pancreatitis has not been elucidated. Here we report that S1PR2 was upregulated in the whole pancreas during acute pancreatitis. Blockade of S1PR2 by pharmacologic inhibition of S1PR2 by JTE-013 or AAV-mediated knockdown of S1PR2 improved the severity of pancreatic injury, as indicated by a significant reduction in inflammation and acinar cells death in acute pancreatitis mice. Moreover, S1PR2 is the predominant S1PRs expressed in pancreatic acinar cells and mediates NF-κB activation and the early inflammatory response within acinar cells under acute pancreatitis conditions via ROCK signaling pathways, not extracellular signal-regulated kinase pathways or p38 mitogen-activated protein kinase pathways. In addition, S1PR2 mediated macrophage NF-κB activation, migration and polarization toward the M1 phenotype. Therefore, these results demonstrated that the S1PR2-mediated early inflammatory response in acinar cells promotes the progression of acute pancreatitis, successfully linking local events to the systematic inflammatory response and leading to a novel therapeutic target for acute pancreatitis aimed at halting the progression of the inflammatory response.
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JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
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2022
·Vol. 332
·期 7
·2473-2485
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IF 1.754
Wang Junlian,Liu Hui,Zhao Hongru,Wang Huajun
A new Zr/Hf separation system was developed with a novel unsymmetrical dialkylphosphinic acid extractant ( n -octyl)(2,4,4'-trimethylpentyl)phosphinic acid (INET-1). It selectively extracts Hf over Zr, and shows much better Zr/Hf separation efficiency than organophosphorous acid Cyanex 272. The Zr and Hf extraction reactions at acidities of < 2 mol/L H 2 SO 4 and > 5.5 mol/L H 2 SO 4 are revealed, respectively. An organic phase with 10 mmol/L INET-1 can load > 290 mg/L of Hf at 1 mol/L H 2 SO 4 . Acidic H 2 SO 4 solutions with concentration of 3.0–4.5 mol/L is a good Zr scrubbing agent.
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Frontiers in Pharmacology
·
2022
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IF 5.988
Nana Li,Lei Dong,Yuanyuan Shen,Yongling Wang,Liansheng Chang,Hongwei Wu,Yuqiao Chang,Menghao Li,Dan Li,Zhaoyi Li,Mei He,Cheng Li,Yao Wei,Haiqin Xie,Feng Wang
In recent years, studies have shown a close relationship between cardiomyocyte death and ferroptosis. Clioquinol (CQ) can inhibit ferroptosis. Porous lipid-poly (lactic-co-glycolic acid) (PLGA) microbubbles (MBs) were prepared by double emulsification (W 1 /O/W 2 ) using 1,2-dioctadecanoyl-sn-glycero-3-phophocholine and PLGA as raw materials. Porous lipid-PLGA MBs were used as carriers to prepare CQ/PLGA MBs containing CQ. CQ/PLGA had the advantages of high drug loading, good biocompatibility, and sustained release. Our results showed that CQ/PLGA improved the effect of CQ and reduced its cytotoxicity. Under low-frequency ultrasound with certain parameters, CQ/PLGA showed steady-state cavitation, which increased the membrane permeability of mouse cardiomyocyte HL-1 to a certain extent and further prevented the process of ferroptosis in mouse cardiomyocyte HL-1.
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JOURNAL OF CATALYSIS
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2022
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IF 8.047
Xin-Xin Fu,Jian-Peng Li,Zhuo-Qian Li,Yan Liu,Chen-Xiao Feng,Hai-Yang Wang,Zhi-Peng Zhao,Qiao-Yun Liu,Zhong-Yi Liu,Zhi-Kun Peng
Biomass is a promising renewable carbon resource, and bio-based platform molecules such as furan derivatives play an important role in the green manufacturing of high value-added chemicals. Of these, the conversion of 2-methylfuran (2-MF) to 3-acetyl-1-propanol (3-AP) over a bifunctional catalyst has attracted significant interest. However, bifunctional catalysts prepared by traditional method suffer from poor catalytic performance and limited stability due to the agglomeration of metal NPs. Here, Pd@HZSM-5, in which Pd NPs were encapsulated in HZSM-5 in situ showed improved stability and 3-AP yield. The highest yield of 3-AP (90.6%) to date was obtained with a nearly 100% conversion of 2-MF. The catalytic performance over a series of Pd@Zeolite confirms the superiority of the encapsulation effect, which is ascribed to the optimal adjustment of metal/acid balance. The Pd@HZSM-5 catalyst displayed excellent stability due to the encapsulation structure, which restricts the Pd NPs agglomeration. The highest yield of 3-AP was obtained at a high concentration of 2-MF (60%), which implies a promising strategy for the green industrial manufacture of 3-AP. This work reports a new bifunctional catalytic system for the green synthesis of 3-AP from 2-MF, thus stimulating the downstream biomass market.
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FUEL PROCESSING TECHNOLOGY
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2022
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IF 8.129
Xin Ning,Junwen Chen,Qi Zuo,Wenlin Li,Jiajun Zheng,Ruifeng Li
Light alkenes oligomerization , performed in the presence of solid acid catalysts , is an important alternative for the production of clean jet fuels. For 1-hexene oligomerization, the current catalyst often suffers from low activity and selectivity. The developed process is upgraded by zeolite catalyst, targeting high-concentration of jet-range hydrocarbons. An efficient and selective IM-5 zeolite-derived catalytic system has been developed for the oligomerization of 1-hexene. The treatment in an aqueous NH 4 F solution led to dealumination without structure degradation. The IM-5 zeolite with a unique two-dimensional IMF structure and 2.5 nm nanoslab showed rapid diffusion ability than ZSM-5 zeolite. Enhance acidities were observed in the NH 4 F-treated IM-5 zeolites owing to fluoride residue and the removal of Al atoms. The strong Brønsted acid sites exerted a positive effect on both the cracking and the oligomerization reactions with the increasing of the yields of dimers. The contact time and partial pressure between the 1-hexene and the IM-5 zeolites were used to analyze the competition of cracking reactions. High 1-hexene conversion (62%), optimal oligomerization to C 3 -C 5 ratio (O/C = 32), and reasonable stability were obtained over the etched IM-5 catalyst at 240 °C, 4 MPa, and the flow rate of N 2 was 50 mL/min.
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Frontiers in Immunology
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2022
·
IF 8.786
Qin Sheng,Huang Xianzhang,Qu Shaogang
Respiratory syncytial virus (RSV) infection is the most frequent cause of hospitalization in pediatric patients. Current systemic treatment and vaccines are not curative and re-infection is often associated with a more drastic incidence of the disease. Baicalin is a flavonoid isolated from Scutellaria baicalensis with potent anti-viral characteristics, namely against RSV. However, its precise mechanism of action remains unclear. Here, using in vitro methods and an in vivo murine model of RSV infection, we showed that baicalin inhibits RSV replication induces translational upregulation of type I interferons (IFNs), IFN-α and IFN-β, and reverses epithelial thickening in lung tissues. Moreover, baicalin inhibits transcription of the RSV non-structural proteins NS1 and NS2. Molecular docking and surface plasmon resonance-based affinity analysis showed that baicalin also binds to the α3 helix of the NS1 protein with an affinity constant of 1.119 × 10−5 M. Polysome profiling showed that baicalin inhibits translation of the RSV matrix protein (M) RNA. Baicalin mediates increased release of the ribosomal protein L13a from the large ribosomal subunit, where the extra ribosomal subunit L13a inhibits M RNA translation. These results comprehensively establish the multiple mechanisms by which baicalin induces a potent innate immune response against RSV infection.
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APPLIED SURFACE SCIENCE
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2022
·
IF 7.392
Mei-Ling Xu,Jia-Ran Li,Xiu-Mei Wu,Ting Yu,Guan-Ying Qin,Fu-Ji Wang,Li-Na Zhang,Kui Li,Xin Cheng
Covalent organic frameworks (COFs) as an emerging porous material have exhibited excellent application prospects in the field of photocatalysis. Nevertheless, the overall water splitting using solar energy by COF-based photocatalysts remains a huge challenge due to the rapid charge recombination and insufficient redox catalytic capacity. Herein, for the first time, the novel Fe-MOF-derived α-Fe 2 O 3 and FeP-PC was designed and adopted as dual co-catalysts to facilitate the separation and utilization efficiency of charge carriers and activate the overall water splitting performance of the ketoenamine based TpPa-1-COF, with both the porous α-Fe 2 O 3 and FeP-PC acting as the framework supporting to effectively prevent the agglomeration of TpPa-1-COF during the fabrication and photocatalytic process. Meanwhile, the construction of Z-scheme heterostructures and the connection of covalent bonds in the interface of α-Fe 2 O 3 /TpPa-1-COF/FeP-PC effectively facilitate the separation and transport of charge. Consequently, the optimal α-Fe 2 O 3 /TpPa-1-COF/FeP-PC excites very competitive photocatalytic overall water splitting performance.
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Journal of Environmental Chemical Engineering
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2022
·
IF 7.968
Xian Liu,Like Chen,Yibo Gao,Jiebin Li,Junyong Sun,Tian Gan
In this context, an innovative design of a molecularly imprinted polymer (MIP) capped Au@HKUST− 1 nanocapsules−based electrode was described for highly sensitive and selective detection of isoproturon herbicide. The Au@HKUST− 1 nanocapsules were constructed by in−situ growth of nanoscaled HKUST− 1 crystals on Cu 2 O core materials and subsequent encapsulation of Au nanoparticles inside the hollow HKUST− 1 walls via galvanic replacement reaction between AuCl 4 − and Cu 2 O. After a further surface imprinting−directing polymerization process in the presence of hydrophobic Au@HKUST− 1, a high−performance electrochemical sensing platform was obtained and used for recognition and quantification of isoproturon. Under the optimal experimental conditions, a linear relationship was observed covering the linear range of 0.0010 − 45 μM with an ultra−low detection limit of 0.45 nM. In addition, the Au@HKUST− 1 @MIP modified electrode had higher sensing specificity for isoproturon in the application of water samples with recoveries in the range of 99.07 − 105.8% and a relative standard deviation of < 4%.
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Theranostics
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2022
·
IF 11.6
Yue Zhou,Lei Xu,Zhangding Wang,Hongwen Liu,Xiang Zhang,Chuanjun Shu,Meng Zhang,Ting Wang,Xinyun Xu,Xiaohong Pu,Jian He,Pin Wang,Yudong Qiu,Guifang Xu,Xiaoping Zou,Yun Zhu,Lei Wang
Rationale : Synergistic treatment strategies for two or more drugs have gradually developed as the main options in clinics for cholangiocarcinoma (CCA) owing to the complicated crosstalk between the tumor and stroma. However, the different synergetic mechanisms pose great challenges to the dosages and order of administration of drugs. Thus, a strategy for exploring and intervening in mutual targets derived from stromal cells and cholangiocarcinoma cells was proposed. Methods : Genes with overexpression patterns in tumors and displaying a significant association with overall survival were identified from RNA-seq data of human CCA patients and CCA mouse models. Western blotting, qRT-PCR, immunofluorescence (IF), colony formation and flow cytometry assays were conducted to determine the biological roles of the key oncogene in cholangiocarcinoma and stromal cells respectively. Additionally, a dual-targeting drug delivery system (AA-HA-ODA) for cancer-associated fibroblasts (CAFs) and tumor cells was constructed to verify the effectiveness of intervening the screened genes in vivo . Results : Polo-like kinase 1 (PLK1) was verified to play vital role in the malignant proliferation of CCA by regulating the cell cycle pathway. PLK1 also decreased stromal production by regulating the CAF phenotype. In addition, a PLK1 inhibitor (Ro3280) loaded dual-targeting drug delivery system (AA-HA-ODA) was prepared and exhibited high affinity for CAFs and cholangiocarcinoma cells. The in vivo distribution pattern and antitumor efficacy of AA-HA-ODA/Ro also verify the effectiveness of inhibiting PLK1 in CCA in vivo . Conclusion : In summary, PLK1 is a mutual target derived from tumor cells and stroma due to its crucial role in the proliferation of tumor cells and stroma regulation in CAFs, which might provide enlightenment for multitarget treatment strategies and guidance for clinical cholangiocarcinoma treatment.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2022
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IF 9.136
Dongli Li,Zhaoqiang Zhang,Liqin Zhou,Youquan Zhang,Zhenxia Zhao,Fang Shen,Xingzhen Qin,Kungang Chai,Hongbing Ji
To efficiently separate acetophenone (AP) and 1-phenyethanol (PE) from petrochemical by-product, we herein report a novel feasible strategy to fabricate β-cyclodextrin (β-CD) based core–shell microsphere, which retains the cage-type crystal structure of β-CD and superior affinity to AP over PE. Using triphenylmethane-4,4′,4′'-triisocyanate (TTI) as monomer, the polymeric TTI shell can grow in-situ on the surface of β-CD particles, giving the core–shell structured CD@PTTI. The unique β-CD core and polymeric TTI shell structure was verified by a series of characterization methods. CD@PTTI-3 exhibits both high AP uptake (ca. 2.07 mmol·g −1 ) and AP/PE selectivity (ca. 7.2), outperforming the conventional TTI cross-linked amorphous β-CD polymer (TTI-CDP). The adsorption behavior of CD@PTTI-3 on AP and PE single-component solutions was investigated by batch adsorption experiments. Based on the experimental and theoretical results, a mechanism of competitive adsorption of AP and PE on CD@PTTI-3 involving host–guest inclusion, hydrogen bonding and π-π stacking is proposed. Furthermore, intense competition between AP and PE on CD@PTTI-3 was observed in column breakthrough experiments, and CD@PTTI-3 could purify AP from 77.16% to 93.6% by a single adsorption–desorption process.
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ORGANIC PROCESS RESEARCH & DEVELOPMENT
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2022
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IF 3.858
Xiangxiang Wang,Youyu Xie,Zhicai Wang,Ke Zhang,Hualei Wang,Dongzhi Wei
We developed a continuous flow system using ω-transaminase covalently immobilized on an epoxy resin modified with ethylenediamine (EDA) to synthesize (S)-1-Boc-3-aminopiperidine, which is a key intermediate for several novel inhibitors. A second functional group (−NH3+) was introduced to rapidly adsorb ω-transaminase, which was further rigidified by multipoint covalent attachment to the modified resin with high binding efficiency (>95%) and activity recovery (75%). The resulting immobilized ω-transaminase was stable with 90% residual activity after 15 reuse cycles. We synthesized (S)-1-Boc-3-aminopiperidine using this continuous flow system, and 95% conversion was obtained within 10 min of residence. The system was continuously operated for 24 h and achieved a space–time yield of 930.73 g·L–1·day–1, indicating outstanding reaction efficiency. This efficient continuous bioprocess has potential for large-scale production of (S)-1-Boc-3-aminopiperidine.
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FUEL
·
2022
·
IF 8.035
Lili Gao,Langlang Qin,Haonan Wu,Xuelian Li,Kai Qi,Qun Yi,Jiandong Zhang,Lijuan Shi
Electrochemical reduction of CO 2 (CO 2 ER) to high value-added multicarbon (C 2+ ) is a viable way to promote carbon–neutral cycle. To date, copper (Cu) is reported as the most effective metal catalyst toward C 2+ , but it remains a challenge to achieve high selectivity and large current density. Incorporating ionic liquid in Cu-based catalysis has been proved an effective strategy, however, most reported methods are either involved too much ionic liquid (IL) as electrolyte or too complex & unstable. Herein, an ionic liquid-embedded Cu electrocatalyst (Cu-IL/GDL) was innovatively constructed through one-step electro-codeposition method, in which a kind of amino-ionic liquid, AFIL, was anchored to the gas diffusion layer (GDL) and embedded by the deposited Cu. Under the excellent synergy between Cu and AFIL, Cu-IL/GDL exhibits higher faradaic efficiency toward ethylene (C 2 H 4, 40.67%) and C 2 (C 2 H 4 and C 2 H 5 OH, 61.39%) when directing CO 2 to carbon-fiber side of electrode in H-cell. To be precise, more Cu (1 0 0) facet is stabilized by the capping effect of AFIL, being in favor for C–C coupling; meanwhile more AFIL + is embedded by the deposited Cu, making for the initiation of CO 2 ER. Thus, this paper proposes a facial and effective strategy to reinforce Cu (1 0 0) facet, and couple and heighten the virtues of ionic liquid and Cu during CO 2 ER toward C 2 .
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