INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2023
·Vol. 58
·期 10
·5332-5345
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IF 3.3
Ruyi Zhu,Zhouyi Xiong,Dan Li
Summary The effect of complex organic acids (COA) treatment at different concentrations (0%, 1%, 5%, 10% and 15%) on the myofibrillar protein (MP) structure and meat quality of crayfish ( Procambarus clarkii ) was investigated. The results showed that with increasing concentrations of COA treatment, the sulfhydryl content of MP decreased and dityrosine content increased. The MP secondary structure has a reduced α-helix content and an increased β-fold content. The MP tertiary structure unfolded, exposing hydrophobic and other amino acids to a polar environment. MP aggregation was induced in the 1% and 5% COA treatment groups and MP degradation was induced in the 10% and 15% COA treatment groups. Crayfish meat in the 5% COA treatment group showed improved textural properties due to protein aggregation. It also enhanced the colour, aroma and micromorphology of the crayfish meat.
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Biotechnology for Biofuels and Bioproducts
·
2023
·Vol. 16
·期 1
·1-12
·
IF 0
Deng Hai-Qing,Lin Xiao-Hui,Fan Jun-Tao,Fu Ping-Zhang,Guan Jia-Jun,Lei Han-Lin,Liu Li-Hao,Lai Lin-Hao,Hou Xue-Dan,Lou Wen-Yong
Background Xylo-oligomers are a kind of high value-added products in biomass fractionation. Although there are several chemical methods to obtain xylo-oligomers from biomass, the reports about the deep eutectic solvents (DESs)-mediated co-production of xylo-oligomers and fermentable sugars and the related kinetic mechanism are limited. Results In this work, glycolic acid-based DESs were used to obtain xylo-oligomers from corncob. The highest xylo-oligomers yield of 65.9% was achieved at 120 °C for 20 min, of which the functional xylo-oligosaccharides (XOSs, DP 2–5) accounted for up to 31.8%. Meanwhile, the enzymatic digestion of cellulose and xylan in residues reached 81.0% and 95.5%, respectively. Moreover, the addition of metal inorganic salts significantly accelerated the hydrolysis of xylan and even the degradation of xylo-oligomers in DES, thus resulting in higher selectivity of xylan removal. AlCl 3 showed the strongest synergistic effect with DES on accelerating the processes, while FeCl 2 is best one for xylo-oligomers accumulation, affording the highest xylo-oligomers yield of 66.1% for only 10 min. Furthermore, the kinetic study indicates that the ‘potential hydrolysis degree’ model could well describe the xylan hydrolysis processes and glycolic acid/lactic acid (3:1) is a promising solvent for xylo-oligomers production, in particular, it worked well with FeCl 2 for the excellent accumulation of xylo-oligomers. Conclusions Glycolic acid-based deep eutectic solvents can be successfully applied in corncob fractionation with excellent xylo-oligomers and fermentable sugars yields on mild conditions, and the large amount of xylo-oligosaccharides accumulation could be achieved by specific process controlling. The strategies established here can be useful for developing high-valued products from biomass.
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CELLULAR SIGNALLING
·
2023
·
IF 4.8
Sijia Han,Xueying Jin,Tianyu Hu,Feng Chi
Non-SMC condensin II complex subunit G2 (NCAPG2) is one of the three non-SMC subunits in condensin II, which plays a vital role in regulating chromosome condensation and segregation. Although the tumor-promoting role of NCAPG2 has been reported in several solid malignancies, its function in breast invasive carcinoma (BRCA) remains unknown. Data both from GEPIA and GSE36295 indicated that NCAPG2 mRNA expression was abnormally upregulated in cancer tissues, which was further verified in 40 paired BRCA and para-carcinoma samples. Kaplan-Meier Plotter further illustrated that BRCA patients with higher NCAPG2 expression have a poorer prognosis. Functional experiments carried out in two BRCA cell lines (MCF-7 and T-47D) showed that NCAPG2-silenced BRCA cells acquired less aggressive behavior — weakened growth and metastasis both in vitro and in vivo. Label-free proteomics quantified the protein expression patterns in MCF-7 cells, and the results revealed 684 differentially expressed proteins (|log 2 FC| > 1 and P < 0.05) downstream to NCAPG2. Interestingly, poly(C)-binding protein 2 (PCBP2), an RNA binding protein previously known to increase RNA stability of its target genes, was found to directly bind to and protect NCAPG2 mRNA from degradation—PCBP2 knockdown accelerated the degradation half-life time of NCAPG2 mRNA from approximately 8 h to 5 h. Taken together, our study indicates that NCAPG2 acts as a novel contributor to BRCA growth and metastasis under the regulation of PCBP2, providing insights into BRCA treatment.
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Current Research in Food Science
·
2023
·
IF 6.3
Tong Jiang,Hong Wang,Peihua Xu,Yifan Yao,Yilong Ma,Zhaojun Wei,Xiangli Niu,Yafang Shang,Dong Zhao
Bread was prepared using wheat flour with grape seed proanthocyanidin (GSP) (0.4%). GSP improved the textural properties of bread including hardness, cohesiviness, gumminess and chewiness. At the last stage of fermentation, GSP reinforced the gluten microstructure with increased the disulfide bonds and hydrophobic interaction and α-helix in the secondary structures. Moreover, GSP addition could increase the total phenolics and antioxidative acitivity of the bread significantly. In addition, the degree of fermentation had a strong influence on the dough forces, and the reasonable control of bread fermentation time was beneficial to improve the bread quality, which provided a reference for the bread processing industry.
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Journal of Cleaner Production
·
2023
·
IF 11.1
Dong Wang,Yumeng Wang,Luheng Jing,Lei Wang,Tonghui Zhang,Xuefen Wang
In this work, a flower-like Fe(OH) 3 @Co 2 (OH) 2 CO 3 catalyst was prepared via the confinement effects provided by L-phenylalanine, which favored the heterostructure construction of active iron centers (Fe(OH) 3 ) anchored on the Co 2 (OH) 2 CO 3 nanosheet supports. Benefiting from the created electron-transfer interface and dual metal active sites, the limitation of redox cycling was broken and exhibited highly efficient catalytic activities , the optimal Fe(OH) 3 @Co 2 (OH) 2 CO 3 catalyst showed comparable catalytic performance for the degradation of dye (degradation degree of 99% methylene blue and orange II within 18 min). In addition, the prepared catalysts were employed for the efficient removal of antibiotics (tetracycline (98.9%), norfloxacin (92.5%) and doxycycline hyclate (90.2%). Besides, the preferred nanofibrous composite membranes prepared by combination of nanofibrous membranes (NFMs) with Fe(OH) 3 @Co 2 (OH) 2 CO 3 catalysts possessed considerable potential for large-scale MB removal (treatment capacity of 238.5 L m −2 and permeability of 191.8 L m −2 h − 1 driven by gravity) and stability (182.7 L m −2 of treatment capacity was achieved in the third run). The strategy of construction of rapid interface-redox on the heterogenous catalysts was designed to enhance catalytic performance, which provided a promising chance for the efficiently large-scale removal of dyes and antibiotics.
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MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 9
·1-14
·
IF 5.7
Mo Guichun,Qin Dongmiao,Wu Yusheng,Luo Zhi,Mo Keting,Deng Biyang
Abstract A new electrochemiluminescence (ECL) cytosensor is proposed for the simultaneous determination of phosphatidylserine (PS) and epidermal growth factor receptor (EGFR) based on the ECL signals of metal-organic framework-5 (MOF-5) loaded CdS quantum dots and N-(aminobutyl)-N-(ethylisoluminol)-polyethylenimine capped Au and Ag nanoparticles. Apoptosis promotes the exposure of PS and reduces the expression of EGFR in cell membranes. Two spatially resolved areas on dual-disk glassy carbon electrodes were designed to eliminate the interference from different ECL probes. Using HepG2 cells treated with resveratrol to induce apoptosis, the cytosensor exhibited high sensitivity, simplicity, and high reproducibility, demonstrating its potential in drug screening and rapid apoptotic cell detection. The strategy reported provides a promising platform for the highly sensitive cytosensing and convenient screening of clinically relevant anticancer drugs. Graphical Abstract
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JOURNAL OF MOLECULAR STRUCTURE
·
2023
·
IF 3.8
Mingya Wang,Shengling Li,Junli Shi,Yongzheng Liu,Duanlin Cao,Linxiu Zhao
A novel, highly sensitive “on-off-on” fluorescent probe ( E )- N ’-((1 H -imidazol-2-yl) methylene) -4-(5, 5-difluoro-1, 3, 7, 9-tetramethyl-5 H -4 λ 4 ,5 λ 4 -dipyrrolo [1, 2- c :2′, 1′- f ] [1,3,2] diazaborinin-10-yl) benzohydrazide ( BDB ) was developed in this study, and its structural and spectral properties were characterized by 13 C NMR, 1 H NMR, X-ray, ESI-MS, ultraviolet and fluorescence photometry. BDB could specifically and efficiently identify Cu 2+ among many other metal ions with a fluorescence quenching efficiency of 92.7% and a recognition limit of 0.65 μM in CH 3 CN/HEPES (7:3, v/v, pH = 7.23) buffer. The energy difference between BDB and BDB -Cu 2+ complex was calculated by DFT. The BDB -Cu 2+ complex could continuously identify GSH in the same system and show an obvious fluorescence enhancement response with a recovery efficiency of approximately 91% and a low recognition limit of 0.02 μM, which is significantly lower than the physiological concentration of GSH in living cells. The applicability of BDB is verified by the strip test. BDB can also be used to detect GSH in HeLa cells, which expand the application of fluorescent chemical sensors in biology.
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LANGMUIR
·
2023
·
IF 3.9
Qianli Sheng,Min Yuan,Dan Wang,Yuanqi Kou,Lei Liu,Yan Chen,Shiyong Song
Vitamin E derivatives are particularly effective in chemotherapy drug development because they are nontoxic, biocompatible, and selective. Among them, α-tocopheryl succinate (α-TOS) can act synergistically with some chemotherapeutic agents. However, its hydrophobicity limits its systemic administration, and localized formulations are not available. Herein, we developed an injectable hydrogel based on self-assembled micelles of a triblock amphiphilic derivative of α-TOS (PEG-2VES), in which doxorubicin (DOX) was encapsulated in the core of the micelles for combined chemotherapy. A molecule of α-TOS was grafted onto each end of poly(ethylene glycols) (PEGs) of different lengths. Hydrogels were prepared by dissolving the polymers or the DOX-loaded micelles in water at room temperature. The subcutaneously injected hydrogels kept their shape and sustainably released the payloads over 7 days without any noticeable inflammatory response. In vitro and in vivo results confirmed the synergistic antitumor effects of the hydrogel and loaded drug. Furthermore, DOX-loaded hydrogels showed greater therapeutic efficiency and fewer toxic side effects than DOX alone. Overall, this hydrogel acts as a multifunctional system that can deliver drug, improve the therapeutic effect, and minimize drug toxicity.
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Journal of Environmental Chemical Engineering
·
2023
·
IF 7.7
Yiqing Liu,Lin Tian,Zhenran Wang,Yongsheng Fu,Dandan Zhao
Citric acid (CA) can effectively complex with Fe(Ⅲ) to improve the solubility of Fe(Ⅲ) and accelerate the reduction of Fe(Ⅲ) to Fe(II), while its use in Fe(Ⅲ)-activated peracetic acid (PAA) system has not been reported so far. Therefore, CA was introduced into Fe(Ⅲ)/PAA system in this study to degrade oxytetracycline (OTC), and the degradation efficiency and mechanism of OTC in this system were systematically investigated. The results showed that the removal efficiency of OTC in Fe(Ⅲ)-CA/PAA system at pH 7.0 could reach 81.5% after 20 min, and the degradation of OTC followed pseudo-first-order kinetic. The addition of CA into Fe(Ⅲ)/PAA system could effectively reduce Fe(Ⅲ) to Fe(Ⅱ) driving the cycle of Fe(Ⅲ)/Fe(Ⅱ) and accordingly enhance the OTC removal in this system. According to the results of electron paramagnetic resonance (EPR) and scavenging experiments, hydroxyl radical (HO • ) and organic radicals (CH 3 C(O)O • and CH 3 C(O)OO • ) played major roles for the degradation of OTC in Fe(Ⅲ)-CA/PAA system. Four possible transformation paths of OTC in this system were proposed, including demethylation, hydroxylation, dehydrogenation and dehydration, based on five detected degradation products . This study provides an idea to improve PAA activation by Fe(Ⅲ) and broadens the practical application of Fe(Ⅲ) in advanced oxidation processes .
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NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 34
·15951-15962
·
IF 3.3
Qingmei Tan,Wensheng Zhang,Tianren Liu,Ying He,Yuangong Ma,Yingxin Yang,Dongxue Han,Dongdong Qin,Li Niu
Owing to the advantages of easy separation and recovery, the development of magnetic photocatalysts is essential to create a viable photocatalytic technology for the degradation of antibiotic contaminants in water. Herein, magnetic Fe3O4@TiO2–Co nanocomposites as a highly recyclable and effective photocatalyst with various Co loading percentages were successfully prepared via a facile route and evaluated for the photodegradation of model tetracycline (TC) antibiotics. As a result, the optimal Fe3O4@TiO2–Co-2.7 sample possesses a removal rate of 92.4% for TC degradation, which is 3.2 fold and 1.6 fold higher than those of single Fe3O4 (28.8%) and Fe3O4@TiO2 (58.7%), respectively. The enhanced photocatalytic activity is mainly attributed to the advantages of integrating magnetic Fe3O4 nanospheres and metal Co nanoparticles, resulting in the overall magnetic composites of Fe3O4@TiO2–Co having an ameliorative light collection ability, energy-saving recycling process, excellent photocatalytic activity, and high chemical and environmental stability. Satisfactorily, the Fe3O4@TiO2–Co photocatalyst could be easily recovered from the reaction solution by an applied magnetic field, and its photocatalytic activity shows almost no loss after five cycles of repetitive use. Moreover, the intermediates in the degradation pathway were identified via high-performance liquid chromatography–mass spectrometry (HPLC-MS) and the TC degradation pathway in the photocatalytic reaction of the as-designed system was proposed. The present work was successfully applied for the purification of tetracycline antibiotics in water pollutants, demonstrating a promising prospect for application to other pollutant degradation systems.
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Materials Today Bio
·
2023
·
IF 8.2
Zhong-Hua Chen,De-Yan Du,Yi-Fei Fu,Jun-Jie Wu,Dan-Yang Guo,Yue-Yue Li,Meng-Nan Chen,Zheng-Dong Yuan,Kai-Wen Zhang,Zhen-Yu Zhang,Xia Li,Feng-Lai Yuan
Bone targeted delivery of estrogen offers great promise for the clinical application of estrogen in the treatment of postmenopausal osteoporosis (PMOP). However, the current bone-targeted drug delivery system still has several issues that need to be solved, such as the side effects of bone-targeted modifier molecules and the failure of the delivery system to release rapidly in the bone tissue. It is important to aggressively search for new bone-targeted modifier molecules and bone microenvironment-responsive delivery vehicles. Inspired by the distribution of citric acid (CA) mainly in bone tissue and the acidic bone resorption microenvironment, we constructed a CA-modified diblock copolymer poly(2-ethyl-2-oxazoline)–poly(ε-caprolactone) (CA-PEOz) drug delivery system. In our study, we found that the CA modification significantly increased the bone targeting of this drug delivery system, and the delivery system was able to achieve rapid drug release under bone acidic conditions. The delivery system significantly reduced bone loss in postmenopausal osteoporotic mice with a significant reduction in estrogenic side effects on the uterus. In summary, our study shows that CA can act as an effective bone targeting modifier molecule and provides a new option for bone targeting modifications. Our study also provides a new approach for bone-targeted delivery of estrogen for the treatment of PMOP.
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DALTON TRANSACTIONS
·
2023
·Vol. 52
·期 29
·10231-10237
·
IF 4
Tonglei Zhang,Wanzhu Wang,Yuyao Zou,Lingnan Li,Danhua Ge,Xiaojun Chen
In this study, we have fabricated a dual-signal sensor for H2O2 determination based on 2D Cu-MOFs decorated with Ag NPs. A novel polydopamine (PDA) reduction method was utilized to reduce [Ag(NH3)2]+ to highly dispersed Ag NPs in situ without other reductive agents, and Cu-MOF@PDA-Ag was obtained. For the electrochemical sensor, the Cu-MOF@PDA-Ag modified electrode exhibits outstanding electrocatalytic properties toward H2O2 reduction with a high sensitivity of 103.7 μA mM−1 cm−2, a wide linear range from 1 μM to 35 mM and a low detection limit of 2.3 μM (S/N = 3). Moreover, the proposed sensor shows good feasibility in an orange juice sample. For the colorimetric sensor, colorless 3,3′,5,5′-tetramethylbenzidine (TMB) can be oxidized by the Cu-MOF@PDA-Ag composite in the presence of H2O2. A Cu-MOF@PDA-Ag catalysis-based colorimetric platform is further established for the quantitative analysis of H2O2 ranging from 0 to 1 mM with a lower detection limit of 0.5 nM. Importantly, such a dual-signal method for the detection of H2O2 could potentially have wide practical applications.
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