Horticultural Plant Journal
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2022
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IF 4.24
Jingming Wang,Mingyue Zhao,Ting Gao,Yingying Feng,Feiquan Wang,Yuting Pan,Jieyang Jin,Tingting Jing,Mengqian Lu,Mengting Zhang,Danyang Guo,Xiaochun Wan,Wilfried Schwab,Chuankui Song
The carotenoid-derived volatile β -ionone makes an important contribution to tea fragrance. Here, we qualitatively and quantitatively analysed 15 carotenoids in tea leaves of 13 cultivars by UHPLC-APCI-MS/MS. The 13 cultivars were divided into two groups by PCA (Principal Component Analysis) clustering analysis of their carotenoid content, and OPLS-DA (Orthogonal projections to latent structures) indicated that the levels of β -carotene (VIP = 2.89) and lutein (VIP = 2.30) were responsible for much of the variation between the two groups. Interestingly, the β -carotene to β -ionone conversion rates in Group 1 were higher than in Group 2, while the β -carotene content was significantly lower in Group 1 than in Group 2. The β -ionone content was significantly higher in Group 1. Pearson Correlation Coefficient calculation between the transcription level of candidate genes ( CsCCD1 and CsCCD4 ) and the accumulation of β -ionone indicated that CsCCD1 may involve in the formation of β -ionone in 13 cultivars. Prokaryotic expression and in vitro enzyme activity assays showed that ‘Chuanhuang 1’ had an amino acid mutation in carotenoid cleavage dioxygenases 1 (CsCCD1) compared with ‘Shuchazao’, resulting in a significantly higher β -ionone content in ‘Chuanhuang 1’. Sequence analysis showed that ‘Chuanhuang 1’ and ‘Huangdan’ had different CsCCD1 promoter sequences, leading to significantly higher CsCCD1 expression and β -ionone accumulation in ‘Chuanhuang 1’. These results indicated that the promoter and coding sequence diversity of CsCCD1 might contribute to the differential accumulation of β -ionone in different tea cultivars.
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JOURNAL OF PHARMACY AND PHARMACOLOGY
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2022
·Vol. 75
·期 1
·129-138
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IF 4.81
Li Shi-ying,Shi Chuan-jian,Fu Wei-ming,Zhang Jin-fang
BackgroundBerberine, a non-prescription medicine clinically applied for diarrhoea and gastroenteritis. Recent studies have demonstrated that it possesses anti-tumour properties in colorectal cancer, but the exact molecular mechanism remains obscure.ObjectivesTo elucidate the underly molecular mechanisms of berberine in colorectal cancer from a perspective of epigenetics, and tried to explore the role of lincROR-Wnt/β-catenin molecular axis in the berberine induced the anti-tumour activity in colorectal cancer.MethodsThe effects of berberine on cell growth, cell cycle and apoptosis were examined in CRC cells. The in vivo effect of berberine on tumour growth was investigated using a xenograft mice model. Moreover, lincROR and Wnt/β-catenin signalling were detected by luciferase activity, qRT-PCR and western blotting assays.Key findingsBerberine suppressed cell growth in vitro via inducing cell cycle arrest and apoptosis in CRC cell, and inhibited tumourigenesis in vivo. LincROR was significantly down-regulated by berberine, inducing the inactivation of the canonical Wnt/β-catenin signalling, meanwhile, the overexpression of lincROR partially reversed the suppressive effects on tumour growth and Wnt/β-catenin signalling induced by berberine.ConclusionsBerberine inhibits tumour growth partially via regulating the lincROR-Wnt/β-catenin regulatory axis, which provides a strategy for the design of anti-tumour drugs for CRC patients after our advanced validation.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2022
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IF 4.831
Kun Ge,Yihong Huang,Hanqiang Zhang
In this paper, we report a novel surface-enhanced Raman spectroscopy (SERS) substrate based on hierarchical β-Bi 2 O 3 /Au 2 Ag 2 microspheres for rapid, sensitive and selective detection of environment pollutants including o -dianisidine ( o -diASD) and Hg 2+ in environmental samples. The sheet-like β-Bi 2 O 3 not only provides large specific surface areas for adsorption of molecules and AuAg, but also emerges as semiconductor matrix with chemical enhancement combined with AuAg with electromagnetic enhancement, making promising SERS activity. Particularly, the β-Bi 2 O 3 /Au 2 Ag 2 shows high SERS performance for 4-mercaptobenzoic acid and TMB with minimum detectable concentration of 0.1 μg/L with enhancement factor of 3.1 × 10 7 and 6.3 × 10 7 , respectively. The density functional theory simulations were further adopted to explain the high SERS activity and selectivity for o -diASD and TMB. Finally, the β-Bi 2 O 3 /Au 2 Ag 2 was applied to direct detection of o -diASD, and indirect detection of Hg 2+ by TMB marking in environmental samples. The linearity range of 0.5–200.0 and 0.2–500.0 μg/L with limit of detection of 0.2 and 0.07 μg/L for o -diASD and Hg 2+ ions can be achieved, respectively. This method provides a novel strategy in designing and fabricating SERS substrates with high performance for rapid, sensitive and accurate analysis of environmental pollutants.
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Food Bioscience
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2022
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IF 5.318
Chen Shen,Wenqing Chen,Changzhu Li,Yong Ye,Haiying Cui,Lin Lin
As a nutritious cold chain food , cheese is susceptible to Listeria monocytogenes contamination. In order to prevent and control Listeria monocytogenes in cheese safely and efficiently, maintain the sensory quality during sale, this paper developed a novel nanofiber membrane with good mechanical strength, hydrophobicity , thermal stability , release performance and bacteriostatic performance as food active packaging. In this study, zein was introduced and electrospun to form a reliable carrier to embed and deliver citral in the cavity of HP-β-CD (Hydroxypropyl-β-cyclodextrin). We found that the zein-based nanofiber membranes showed preferable tensile properties and hydrophobicity with 4% (w:v) addition of citral/HP-β-CD inclusion complex. Meanwhile, the thermal stability and release property of citral were significantly improved by embedding in HP-β-CD and loading into zein-base nanofiber membranes. Moreover, after packaged by Zein nanofiber membrane encapsulated with citral/HP-β-CD inclusion complex under the storage conditions of 4 °C and 12 °C, the growth of Listeria monocytogenes in four kinds of cheeses was inhibited to a certain degree. The texture and sensory quality of the four cheeses at the two temperatures and the color quality under the low temperature condition of 4 °C did not change significantly. In summary, Zein nanofiber membranes encapsulated with citral/HP-β-CD inclusion complex has potential application prospects in the field of food active packaging.
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MICROCHIMICA ACTA
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2022
·Vol. 189
·期 8
·1-10
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IF 6.408
Wang Jiawei,Du Yi,Du Jianxiu
A new fluorescence method was established for sensitive detection of β-galactosidase (β-gal) activity in spiked human serum and screening of inhibitor. Nitrogen-doped carbon quantum dots (N-CQDs) were prepared by solvothermal polymerization of N-methyl-2-pyrrolidinone in an alkaline condition. The colloidal N-CQDs exhibit good water solubility, stability, and emit bright green fluorescence with a maximum emission peak at 528 nm upon excitation at 420 nm. β-gal specifically catalyzes the decomposition of its substrate P-nitrophenyl-β-D-galactopyranoside into 4-nitrophenol, whose absorption spectrum overlaps well with the excitation spectrum of the N-CQDs. As a result, the fluorescence of the N-CQDs is remarkably quenched by 4-nitrophenol via an inner filter effect. The sensing platform presents a linear response range for β-gal activity from 0.05 to 3.0 U·L −1 with a low limit of detection of 0.023 U·L −1 . An acceptable precision is obtained with a relative standard deviation (RSD) of 3.1% for 1.0 U·L −1 β-gal ( n = 11). The method was applied to determine β-gal in spiked human serums with recoveries in the range 96.3–104.7%. The method was employed to evaluate inhibitor screening with D-galactal and chloroquine diphosphate as models. Graphical abstract
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SEPARATION AND PURIFICATION TECHNOLOGY
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2021
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IF 7.312
Jian Zhang,Yahui Liu,Wensen Liu,Lina Wang,Jing Chen,Zhaowu Zhu,Tao Qi
The synergisms of β-diketone and phosphine oxide are very efficient for lithium selective extraction from alkaline systems, and have been obtained wide applications, especially for lithium recovery from salt lake brines. However, the systems are ready to form emulsification which seriously impacted their practical applications. In this study, the synergistic mechanism of β-diketone and phosphine oxide was intensively studied using a typical β-diketone of HTTA (2-Thenoyltrifluoroacetone) and TOPO (Trioctylphosphine oxide) through extraction parameter relationships, FT-IR measurements, structure computational simulations. It is found that one molar water readily presented in the extracted species forming Li·(H 2 O)·TTA·TOPO, while two molar water presented when HTTA alone was used which more readily forms emulsification or even solid. When TOPO was attended, one molar of water was replaced by it as there is an unconfirmed “boundary“ formed between them which will significantly mitigate the emulsification formation.
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DRUG DELIVERY
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2021
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IF 6.419
Jianbo Li,Tiange Feng,Weijing Yang,Yaru Xu,Shuaishuai Wang,Huijie Cai,Zhilei Liu,Hong Qiang,Jinjie Zhang
Although Fraxinellone (Frax) isolated from Dictamnus albus L. possessed excellent anti-hepatic fibrosis activity, oral administration of Frax suffered from the inefficient therapeutic outcome in vivo due to negligible oral absorption. At present, the oral formulation of Frax is rarely exploited. For rational formulation design, we evaluated preabsorption risks of Frax and found that Frax was rather stable while poorly dissolved in the gastrointestinal tract (78.88 μg/mL), which predominantly limited its oral absorption. Further solubility test revealed the outstanding capacity of cyclodextrin derivatives (CDs) to solubilize Frax (6.8–12.8 mg/mL). This led us to study the inclusion complexes of Frax with a series of CDs and holistically explore their drug delivery performance. Characterization techniques involving 1H-NMR, FT-IR, DSC, PXRD, and molecular docking confirmed the most stable binding interactions when Frax complexed with 6-O-α-D-maltosyl-β-cyclodextrin (G2-β-CD-Frax). Notably, G2-β-CD-Frax exhibited the highest solubilizing capacity, fast dissolution rate, and superior Caco-2 cell internalization with no obvious toxicity. Pharmacokinetic studies demonstrated markedly higher oral bioavailability of G2-β-CD-Frax (5.8-fold that of free drug) than other Frax-CDs. Further, long-term administration of G2-β-CD-Frax (5 mg/kg) efficiently inhibited CCl4-induced hepatic fibrosis in the mouse without inducing any toxicity. Our results will inspire the continued advancement of optimal oral Frax formulations for anti-fibrotic therapy.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2021
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IF 3.764
Zhongwen Dong,Xinyi Wang,Weifeng Chu,Chuanyu Yang,Yanan Wang,Lin Mei,Dongpu Zhao,Wenjie Xin,Shenglin Liu,Hongxia Wang,Yongchen Shang,Longya Xu
A fast synthetic methodology of β-zeolite with the assistance of γ-butyrolactone (GBL) has been developed in this paper, and the synthetic parameters are systematically investigated to obtain β-zeolite efficiently. Compared to the conventional synthetic system with a crystallization time of 120 h, GBL promotes the crystallization of β-zeolite in a much shorter time of 24 h. An accelerated nucleation mechanism of β-zeolite assisted by GBL is unraveled by X-ray diffraction, scanning electron microscopy, thermogravimetry–differential thermal analysis, Fourier transform infrared spectroscopy, and NMR techniques. The results demonstrate that GBL can adjust the pH value of the initial gels and enhance the combination of TEA+ with aluminosilicate species; in the meantime, the Hoffmann degradation of TEAOH is suppressed. In addition, the β-catalyst obtained with the assistance of GBL exhibits both a higher selectivity of tert-butylbenzene and excellent catalytic stability in the alkylation of benzene with isobutylene compared with the commercial β-catalyst with a similar SiO2/Al2O3 molar ratio.
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RSC Advances
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2021
·Vol. 11
·期 57
·35754-35764
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IF 3.361
Ning Zhang,Siyu Guo,Bolin Gong
A bridged bis(β-cyclodextrin) ligand was firstly synthesized via a thiol–ene click chemistry reaction between allyl-ureido-β-cyclodextrin and 4-4′-thiobisthiophenol, which was then bonded onto a 5 μm spherical silica gel to obtain a novel bridged bis(β-cyclodextrin) chiral stationary phase (HTCDP). The structures of HTCDP and the bridged bis(β-cyclodextrin) ligand were characterized by the 1H nuclear magnetic resonance (1H NMR), solid state 13C nuclear magnetic resonance (13C NMR) spectra spectrum, scanning electron microscope, elemental analysis, mass spectrometry, infrared spectrometry and thermogravimetric analysis. The performance of HTCDP in enantioseparation was systematically examined by separating 21 chiral compounds, including 8 flavanones, 8 triazole pesticides and 5 other common chiral drugs (benzoin, praziquantel, 1-1′-bi-2-naphthol, Tröger's base and bicalutamide) in the reversed-phase chromatographic mode. By optimizing the chromatographic conditions such as formic acid content, mobile phase composition, pH values and column temperature, 19 analytes were completely separated with high resolution (1.50–4.48), in which the enantiomeric resolution of silymarin, 4-hydroxyflavanone, 2-hydroxyflavanone and flavanone were up to 4.34, 4.48, 3.89 and 3.06 within 35 min, respectively. Compared to the native β-CD chiral stationary phase (CDCSP), HTCDP had superior enantiomer separation and chiral recognition abilities. For example, HTCDP completely separated 5 other common chiral drugs, 2 flavanones and 3 triazole pesticides that CDCSP failed to separate. Unlike CDCSP, which has a small cavity (0.65 nm), the two cavities in HTCDP joined by the aryl connector could synergistically accommodate relatively bulky chiral analytes. Thus, HTCDP may have a broader prospect in enantiomeric separation, analysis and detection.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2021
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IF 5.279
Huicui Liu,Tao Chen,Xiaoqing Xie,Xinlei Wang,Yiwen Luo,Nan Xu,Zhen Sa,Min Zhang,Zhifei Chen,Xinzhong Hu,Juxiu Li
Nonalcoholic fatty liver disease (NAFLD) is characterized by marked imbalances in lipid storage and metabolism. Because the beneficial health effects of cereal β-glucan (BG) include lowering cholesterol and regulating lipid metabolism, BG may alleviate the imbalances in lipid metabolism observed during NAFLD. The aim of our study was to investigate whether BG from highland barley has an effect on western diet-induced NAFLD in mice. Using lipidomics, we investigated the underlying mechanisms of BG intervention, and identified potential lipid biomarkers. The results reveal that BG (300 mg/kg body weight) significantly alleviated liver steatosis. Lipidomics analysis demonstrated that BG also altered lipid metabolic patterns. We were able to identify 13 differentially regulated lipid species that may be useful as lipid biomarkers. Several genes in the hepatic lipid and cholesterol metabolism pathways were also modulated. These findings provide evidence that BG ameliorates NAFLD by altering liver lipid metabolites and regulating lipid metabolism-related genes.
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Food Bioscience
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2021
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IF 4.24
Kehong Hou,Yaoyao Xu,Kaiyue Cen,Chengcheng Gao,Xiao Feng,Xiaozhi Tang
Essential oils (EOs) are gaining great interest as alternatives to synthetic food preservatives due to their antimicrobial activity . However, EOs are hydrophobic and easy to evaporate. In this research, EOs were encapsulated in nanoemulsions with the aid of hydroxypropyl-β-cyclodextrin (HPCD) to protect the ingredients and sustain the release, so as to exert long-term antibacterial effects. The influence of HPCD and its concentration on cinnamon essential oil (CEO) nanoemulsion formation, antibacterial activity , release property and stability was investigated. The results showed that HPCD reduced the particle size of nanoemulsion as the concentration of Tween 80 ranged from 2 to 4 g/L. However, HPCD addition showed adverse effect as the content of Tween 80 was 5 g/L. Antibacterial tests against Escherichia coli and Staphylococcus aureus demonstrated that the addition of HPCD contributed to the increase of antimicrobial activity of CEO nanoemulsions and showed the best effect when the concentration of HPCD was 30 g/L. The release experiment proved the slow-release ability of HPCD, and showed that the cavity of HPCD helped to reduce the loss of CEO, thus delayed the release time and enhanced the antibacterial effect. Meanwhile, the use of HPCD contributed to the storage stability of the emulsion at 4, 25, and 40 °C, and the nanoemulsion remained stable during a storage period of 60 days.
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
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2021
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IF 5.875
Wenjing Su,Nikolay E. Polyakov,Wenhao Xu,Weike Su
This research aimed to overcome the current challenges in the application of natural carotenoid antioxidants, such as their complex preparation processes, low bioavailability and poor drug stability. Herein, a mechanochemical method was used to prepare an inclusion complex (IC) that self-assembles into micelles in aqueous solution and achieves solid-phase loading of astaxanthin (AST). The NMR analysis, thermodynamics study, particle size analysis and electron microscopy image results showed that AST, hydroxypropyl β-cyclodextrin (HPβ-CD) and glyceryl monostearate (GMS) formed self-assembled micelles and maintained good stability in aqueous solution. The antioxidant performance experiments showed that the formation of IC increases free radical scavenging activity. The pharmacokinetic studies showed that the bioavailability of the astaxanthin inclusion complex increased 4-fold. The tissue distribution experiments showed that the astaxanthin inclusion complex targets the liver to exert its antioxidant effects. The proposed method uses an innovative preparation technology to produce an efficient drug delivery system without solvents, and it exerts powerful antioxidant activity against astaxanthin.
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