npj Clean Water
·
2025
·
IF 11.4
Lv Shiyu,Yuan Tian,Zhang Xueyan,Yang Pu,Fu Kerong,Yao Xia,Fu Weilin,Zhang Qiuxian,Luo Yanli,Wang Feng
Herein, LaFeO 3 photocatalysts (HX-LFO and SX-LFO) were synthesized using hydrothermal and sol–gel methods, respectively, for removing β-E2 from dairy cattle breeding wastewater. A combination of flocculation pretreatment and photocatalytic Fenton oxidation was used to achieve efficient β-E2 degradation. Results revealed that HX-LFO had high defect concentrations and achieved better photocatalytic Fenton oxidation efficiency for β-E2 than SX-LFO. The H0.7-LFO/H 2 O 2 /visible (Vis) system completely degraded 2 mg/L of β-E2 within 2 h and achieved a total organic carbon (TOC) mineralization rate of 99.7%. The intermediate products, degradation pathways, and ecological toxicity of β-E2 degradation were analyzed. The H0.7-LFO/H 2 O 2 /Vis system exhibited stable degradation performance when humic acid and multiple anions coexisted in the reaction solution. The developed system increased the β-E2 degradation rate to 83.38%–100%. Characterizations and density functional theory calculations revealed that the La-defect formation energy in La/O dual-defect LaFeO 3 was lower than that in LaFeO 3 containing only La defects. Moreover, O defects facilitated the formation of La defects that lowered the energy barrier for free-radical desorption, making it easier for free radicals to form and diffuse into the reaction solution. La/O dual-defect LaFeO 3 exhibited lower binding energy for β-E2, accelerating charge transfer within itself and from itself to β-E2.
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Cell Death Discovery
·
2025
·Vol. 11
·期 1
·555
·
IF 7
Deng Zhilin,Huang Shuying,Pei Yu,Li Hanxiang,Dong Yong,Li Yuanyuan,Ran Qin,Liu Xindong,Feng Yi,Wang Qiang,Guo Zhenhua,Huang Sizhou
The proliferation of early erythrocyte and the subsequent maturation are critical events during erythropoiesis, while how these two independent but interconnected processes are efficiently orchestrated during erythropoiesis is largely unknown. Prpf4 expression is enriched from Pre-Colony Forming Unit-Erythroid (PreCFU-E) to Nucleated Erythrocytes, especially in the CFU-E cells, implying that Prpf4 plays a critical role in erythropoiesis. Here, we demonstrate that prpf4 sequentially regulates erythrocyte proliferation and maturation during zebrafish definitive hematopoiesis. The data show that prpf4 mutation results in severe defects in erythropoiesis, characterized by a substantial reduction in erythroid cell numbers and impaired erythrocyte maturation. Further analysis indicates that prpf4 mutation leads to cell cycle arrest of erythrocytes at the S and G2/M phases, as well as a significant increase in erythrocyte apoptosis. Mechanistically, prpf4 mutation leads to DNA damage and the subsequent activation of the DNA damage response, triggering the ATM/CHK2-p53 signaling pathway. This process inhibits the proliferation of early erythrocyte and induces erythrocyte apoptosis. On the other hand, the data reveal that prpf4 mutation causes significant defects in skipped-exon during pre-mRNA splicing, accompanied by severe splicing defect in slc25a39 pre-mRNA. This results in a significant downregulation of slc25a39 mRNA, which partially impairs erythrocyte maturation during late erythropoiesis. In conclusion, we identify that prpf4 sequentially regulates early erythrocyte proliferation and subsequent erythrocyte maturation. This dual function of prpf4 partially explains how early erythrocyte proliferation and late maturation are efficiently coordinated during erythropoiesis.
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AQUACULTURE INTERNATIONAL
·
2025
·Vol. 33
·期 7
·1-22
·
IF 2.4
Chen Zhengxu,Jing Futao,Zhang Tengwen,Yu Xin,Liao Liwen,Li Yaobing,Wen Yuxin,Pan Luqing
This study integrates network pharmacology, molecular docking, and a 56-day feeding trial to evaluate the effects of dietary pterostilbene (PTE) on growth, physiological status, and underlying regulatory pathways in Litopenaeus vannamei . The results indicate that dietary supplementation with a specific dose of PTE enhances shrimp growth, improves metabolic capacity, boosts antioxidant defenses, strengthens immune responses, and reduces inflammation. The regulatory mechanism of PTE involves targeting and binding to key proteins in lipid metabolism-related signaling pathways, including AKT , INSR , and SIRT1 within the insulin and SIRT1 - AMPK pathways. This binding significantly increases the gene expression levels of these targets ( P < 0.05), thereby activating the respective signaling pathways and enhancing glycolipid metabolism capacity. In terms of immune function, PTE targets and binds to key antioxidants and immune proteins, including Nrf2 , Relish , and Dorsal , resulting in a significant increase in the expression levels of these genes ( P < 0.05). Consequently, the expression of downstream antioxidant enzymes associated with Nrf2 ( SOD , CAT , GPX ) was significantly elevated ( P < 0.05), enhancing antioxidant capacity. Additionally, the expression of antibacterial lysosomal factors ( PEN3 , CRU , ALF , and LYC ) also increased ( P < 0.05), thereby strengthening immune function. Moreover, dietary PTE reshapes the structure of gut microbiota and increases the abundance of beneficial bacteria. This study elucidates the molecular mechanisms and optimal dosage of dietary PTE in improving the physiological function of shrimp by combining molecular docking with in vivo findings, providing a theoretical basis for the development of efficient and safe aquaculture feed additives.
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CELLULOSE
·
2025
·
IF 4.8
Zheng Mengjie,Guo Qingfeng,Yue Yizhi,Lu Chaoyang,Ran Jianhua,Bi Shuguang
A multifunctional cotton fabric was fabricated by in-situ synthesis of zeolitic imidazolate framework-8 (ZIF-8) on a polydopamine (PDA)-modified cotton substrate. Leveraging the reducing capability of PDA, silver nanoparticles (Ag NPs) were simultaneously incorporated into and around the ZIF-8 nanocrystals. The successful fabrication of the Ag/ZIF-8 functional cotton fabric was confirmed by characterization techniques including SEM, XRD, XPS, and TG. The modified fabric exhibits exceptional photocatalytic performance and cycling stability, achieving 93.4% degradation of methylene blue (MB) under visible light irradiation within 170 min and retaining 90.2% efficiency after three degradation cycles. Furthermore, the synergistic effect between Ag and ZIF-8 endows the fabric with significant antibacterial activity and efficient heavy metal ion adsorption capacity. Therefore, this study provides a novel strategy for designing multifunctional materials, holding significant promise for advancing the development of multifunctional textiles.
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JOURNAL OF WUHAN UNIVERSITY OF TECHNOLOGY-MATERIALS SCIENCE EDITION
·
2025
·Vol. 40
·期 6
·1782-1789
·
IF 1.1
Cai Yao,Wu Hongmei
This article provided a preparation protocol for poly(lactic acid) (PLA)/modified epoxidized soybean oil (ECP)/nano-magnesium oxide(n-MgO) ternary composites and studied their mechanical and antibacterial properties. By means of an organic synthesis technique, epoxidized soybean oil (ESO) is chemically grafted to PLA to synthesize ESO chemically plastically modified PLA, abbreviated ECP. To fabricate PLA/ECP/n-MgO composite materials, ECP acts as a plasticizer and a compatibilizer simultaneously, and n-MgO acts as an enhancer. Then scanning electron microscopy, X-ray diffraction, differential scanning calorimetry, universal tester, and antibacterial research were exploited to characterize the morphology, thermal resistance, mechanical properties, and antibacterial performance of PLA/ECP/n-MgO composites. The experimental results show that ECP acts as a plasticizer by causing heterogeneous nucleation, which increases PLA’s crystallinity. Evenly distributed n-MgO can greatly improve PLA’s antibacterial qualities. Furthermore, ECP and n-MgO work together to improve the positive aspects of PLA/ECP/n-MgO composites, with PLA/ECP/n-MgO 100/1/0.5 composites having the best overall properties. While improving the mechanical performance and toughness of PLA, this work offers a prospective approach and foundational database for the creation of multifunctional biodegradable composites.
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Nature Communications
·
2025
·Vol. 16
·期 1
·10644
·
IF 15.7
Zhao Shanshan,Zhang Mingjiang,Li Anqi,Tong Zhi,Li Guangen,Guo Qi,Zhou Yajie,Wang Yaxin,Cai Junjie,Zhuang Taotao
Visualization in complex, extreme environments necessitates that the imaging material/system to deliver more comprehensive information and endures external mechanical deformation. Endowing polarization, for example, helicity, in adaptable optical materials—to construct circular-polarization-enabled imaging—offers the chance to address the issue. However, retaining considerable luminescence asymmetry during processing while behaving like an elastic rubber remains challenging, resulting from unstable circularly polarized luminescence when deforming existing polarization materials. Here, we propose a covalent cross-linking strategy, combining a sustainable light generator with an optical helicity modulator, to prepare chiroptical network structures exhibiting superior mechanical-chiroptical coupling. The obtained structures provide satisfying circularly polarized luminescence with an asymmetry factor up to 1.31 alongside exceptional tensile strain tolerance, and more importantly, the asymmetry maintains a magnitude of 10 −1 even with 80% deformation. We further advance our materials into wearable polarization optical sensors, practicing our developed circular polarization differential imaging in a fire scenario, showing an advancement in imaging resolution compared to other technologies such as thermal, intensity and fluorescence imaging. This covalent-bonding chiroptical network structures enable deformable optical helicity, paving a way for achieving portable imaging identification under extreme conditions.
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BRAZILIAN JOURNAL OF CHEMICAL ENGINEERING
·
2025
·
IF 1.5
Jiang Yu-cheng,Hai Jun-qi,Zhu Di,Long Xiang-li
The commercial production of benzaldehyde through the toluene chlorination and hydrolysis method not only releases chloride-laden effluents into the environment but also excludes its application into the field of food and medicine due to the residual chlorine. To develop a green process that produces chlorine-free product efficiently and cheaply, a keggin-type polyoxometalate designed with Co as a heteroatom, Mo, W and Mn as ligand atoms and La as a counter cation has been loaded on activated carbon to catalyze the production of benzaldehyde from the oxidation of toluene in liquid-phase by oxygen. The optimum catalyst preparation conditions are as follows: polyoxometalate loading 35%(wt), pH 1 = 7.1, pH 2 = 3.8, T 1 = T 2 = 85 °C and activated in N 2 at 220 ℃ for 4 h. Characterization has been done with ICP-OES, FT-IR, XRD and TG. The polyoxometalate synthesized is proved to be Keggin-type structure. The catalyst Na 4.2 LaCoMn 0.3 W 1.5 Mo 10.2 O 40 ·18.53H 2 O@AC manifests the best performance in catalyzing the production of benzaldehyde from the partial oxidation of toluene with a toluene conversion of 18.55%, benzaldehyde selectivity of 33.61% and benzaldehyde yield of 6.23%.
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Scientific Reports
·
2025
·Vol. 15
·期 1
·39418
·
IF 3.9
Liu Huan,Zou Qin,Xiang Tao,Zhu Rui,Chen Zigui,Chen Rui
Subarachnoid hemorrhage (SAH) is a common cerebrovascular disease that can lead to cognitive impairment. Although tetrandrine (Tet) has been proposed as a potential therapeutic agent, its efficacy in the treatment of SAH has not been fully explored. To investigate Tet effects on SAH, a rat model was established and divided into an SAH + vehicle and an SAH + Tet group. Cognitive function and behavioral performance were assessed using the Morris water maze and open field tests. Inflammatory cytokine levels were measured by ELISA, and hippocampal injury was evaluated by hematoxylin-eosin (HE) staining. Neuronal loss was quantified using Nissl staining, while neuronal density was assessed via immunofluorescence. The expression of the TLR4/NF-κB signaling pathway related-proteins was examined by Western blotting, and 16 S rRNA sequencing was conducted to determine differences in gut microbiota composition across the groups. Tet treatment significantly reduced mNSS and SAH assessment scores in SAH rats, suggesting an improvement in neurological and cognitive function. Behavioral analysis demonstrated that Tet increased escape latency, movement speed, and total distance in the Morris water maze. Histological staining revealed attenuated hippocampal damage and decreased neuronal death in the Tet-treated group. These neuroprotective effects were accompanied by reduced expression of TLR4 and NF-κB pathway components, as well as decreased secretion of LPS, TNF-α, IL-1β, and IL-6. Notably, Tet modulated the gut microbiota, restoring microbial diversity and abundance, and this modulation was associated with changes in the CYP51 metabolic pathway. Tet improves cognitive function and reduces neuronal injury in SAH rats by regulating the gut microbiota and its associated CYP51 metabolic pathway, thereby suppressing activation of the TLR4/NF-κB signaling cascade.
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Probiotics and Antimicrobial Proteins
·
2025
·
IF 4.4
Huang Fang,Lu Yun-hui,Xiong Jie,Zeng Xin-an,Huang Yan-yan,Wang Lang-hong
The escalating global burden of hyperuricemia, driven by modern dietary patterns and sedentary lifestyles, has prompted urgent exploration of novel therapeutic strategies, with particular emphasis on developing targeted probiotic interventions as a potential alternative to conventional pharmacological approaches. This study successfully isolated Limosilactobacillus fermentum FOSU YHD19 from fermented foods, demonstrating remarkable in vitro uric acid reduction of 12.90% along with high-level degradation of nucleosides. A comprehensive safety evaluation confirmed the absence of harmful enzymatic activities (including nitroreductase, decarboxylase and tryptophanase), hemolytic properties, or antibiotic resistance. The strain exhibited strong stress tolerance, surviving in acidic conditions (pH 2.0), with exposure to gastric juice, and when in the presence of 0.1% bile salts. Additionally, YHD19 demonstrated potent antibacterial activity against multiple pathogenic strains, including E coli , S. aureus , L. monocytogenes , and S. enteritidis . Genomic analysis revealed three nucleoside hydrolases and six transporter systems that collectively facilitate the hypouricemic activity. By ruling out the presence of key uric acid-synthesizing enzymes (xanthine dehydrogenase/oxidase and guanine deaminase), we can attribute the hypouricemic effect primarily to a nucleoside-scavenging mechanism. In addition, the genome harbors 91 stress-responsive genes implicated in acid/base adaptation, thermal shock response, and oxidative defense mechanisms, underscoring the strain’s resilience under gastrointestinal stresses. These findings position YHD19 as a safe and effective probiotic candidate for functional foods aimed at managing hyperuricemia, supporting its safety profile for potential probiotic applications.
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Journal of Translational Medicine
·
2025
·Vol. 23
·期 1
·1349
·
IF 7.5
Zhu Ganggui,Li Qiaxuan,Yao Qifeng,Zhao Jin,Ding Shuo,Mai Shixiong,Yue Bingqin,Chen Jingyu,Huang Man
Background Lung ischemia-reperfusion injury (LIRI) is associated with a variety of postoperative complications and affects patient prognosis. β-Nicotinamide mononucleotide (NMN) has attracted much attention due to its potential role in cellular energy metabolism and antioxidant properties. Exploring the mitigating role of NMN in ischemia-reperfusion injury after lung transplantation is crucial for improving lung transplantation success and patient quality of life. Methods A LIRI model was established using the mouse left lung portal clamp method, and the cold ischemia-reperfusion (CI/R) model with BEAS-2B cells in vitro was used to simulate the lung transplantation environment. The protective effect of NMN against lung ischemia-reperfusion injury was analyzed by histopathology, ELISA, immunofluorescence, Western blot and RT-PCR. Combined multi-omics profiling were used to study the mechanisms by which NMN attenuated lung ischemia-reperfusion injury. Results NMN significantly attenuated ischemia-reperfusion-induced structural abnormalities, pulmonary edema, inflammatory infiltration and oxidative stress injury in mouse lung tissue. Similarly, NMN attenuated CI/R-induced oxidative stress and inflammatory responses in BEAS-2B cells. Based on multi-omics analysis, we found that NMN ameliorated the apoptosis after ischemia/reperfusion through down-regulation of cleaved Caspase 3, BAX and up-regulation of BCL-2. Moreover, NMN also affected mitochondrial metabolic processes, including promoting mitochondrial oxidative phosphorylation, increasing ATP synthesis, enhancing mitochondrial complex activity, decreasing ROS generation, slowing down the decrease of mitochondrial membrane potential, and inhibiting the opening of the mitochondrial membrane permeability transition pore. Mechanistically, we found that NMN attenuates inflammation and oxidative damage through activation of the SIRT1 and NF-κB signaling pathways, and that SIRT1-specific inhibition of EX527 reversed these protective effects of NMN against inflammation and oxidative damage in vivo and in vitro. Conclusion NMN has a protective effect on inflammation and oxidative damage induced by ischemia-reperfusion injury after lung transplantation, and the SIRT1/NF-κB pathway may be involved in one of the mechanisms of the protective effect.
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Nature Communications
·
2025
·Vol. 16
·期 1
·10551
·
IF 15.7
Meng Qingcai,Li Chengxi,Cai Yuping,Chen Ying,Chen Xiaoqing,Wang Xin,Zhang Biling,Zhang Yue,Liu Feng,Chen Meixin
Itaconate accumulates in macrophages upon bacterial infection, and manifests antibacterial activity. Convincing evidence substantiates that itaconate is transported across the plasma membrane and vacuolar membrane, but the molecular bases underlying bidirectional transport of itaconate across membranes and its effects on intracellular bacterial replication are less known. Here, we identify MCT1 and MCT4 as bidirectional transporters of itaconate. In addition to modulating itaconate concentration as transporters at the plasma membrane, MCT1 and MCT4 function as itaconate transporters at Salmonella -containing vacuole (SCV). Upon Salmonella infection, MCT1 and MCT4 transport itaconate into SCV facilitated by RAB32. Itaconate is also secreted out of cells through MCT1 and MCT4 as the infection persists. The suppression of MCT1 and MCT4-dependent itaconate secretion increases the overall concentration of itaconate and the proportion of itaconate-targeted Salmonella intracellularly, consequently inhibiting Salmonella replication. Our study thus offers valuable insights into itaconate transport during bacterial infection and provides proof of principle for the development of itaconate-dependent therapeutic strategies.
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BMC Pulmonary Medicine
·
2025
·Vol. 25
·期 1
·1-11
·
IF 2.8
Luo Jun,Liu Min,Ai Wen,Zheng Xiaoling,Han Lu,Huang Kuo,Zhang Changlin,Wang Peng,Fan Jinhong,Li Qianyuan,Luo Chunhua
Background Timely detection of viable Staphylococcus aureus (MSSA)/ methicillin-resistant Staphylococcus aureus (MRSA) and antimicrobial resistance are crucial for the treatment of patients with pulmonary infection. Methods In the current study, we have developed and evaluated the potential clinical applicability of the PMA TaqMan-based real-time PCR (PMA-TaqMan qPCR) assay for detection of viable MSSA/MRSA and antimicrobial susceptibility directly from bronchoalveolar lavage fluid. Results The PMA-TaqMan qPCR assay demonstrated high sensitivity with a detection limit of 800 CFU/mL within 115 min. Furthermore, the assay required only 45 min of antibiotic exposure and completed antimicrobial susceptibility testing within 160 min. Upon detection of 10 spiked bronchoalveolar lavage fluid samples treated by clindamycin after 24 h, the PMA-TaqMan qPCR assay showed better agreement with culture-based methods in detecting viable bacteria. Subsequent antimicrobial susceptibility results of bronchoalveolar lavage fluid samples spiked by 16 MRSA isolates and 11 MSSA isolates based on the current assay, compared with the standard broth microdilution method, showed an overall agreement of 100% (95% confidence intervals [CI]: 80.64 to 100) for MRSA and 90.91% (95% CI: 62.27 to 98.38) for MSSA. Conclusions Our findings demonstrate that the PMA-TaqMan qPCR assay is a highly sensitive and specific method for the direct detection of viable MSSA/MRSA and antimicrobial susceptibility testing from bronchoalveolar lavage fluid.
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