GREEN CHEMISTRY
·
2025
·
IF 9.2
Yumei Chen,Chunhui Xie,Yang You,Tonghui Xu,Yunqi Li,Jili Yuan,Haibo Xie,Yuanlong Guo
The demand for the recovery of precious metals has become increasingly prominent due to their scarcity and wide application, while the facile preparation of sustainable absorption and separation materials via molecular engineering still face great challenge by using renewable polymer resources. Herein, a water-soluble cellulose levulinate ester (CLE) with ketocarbonyl groups was utilized as 3D network scaffold to fabricate a cellulose-based aerogel adsorbent (CLE@PEI) by introducing polyethyleneimine (PEI) with multiple amine groups. The abundant functional groups and porous architecture allowed CLE@PEI to efficiently capture Au(III), Pt(IV) and Pd(II). The maximum adsorption capacity of CLE@50PEI reached a staggering 1752.0 mg/g for Au(III) at 298 K which was superior than that of Pt(IV) (1420.5 mg/g) and Pd(II) (495.1 mg/g). The adsorption process followed pseudo-second-order kinetic model and Langmuir isotherm behavior, respectively. Additionally, the positively charged CLE@50PEI exhibited excellent selectivity for precious metals in multi-metal mixtures. The outstanding adsorption performance of the CLE@50PEI may be due to the new “adsorption-in situ reduction” mechanism, in which the process primarily involved the ion exchange coupled with electrostatic interaction and complexation, and the in situ reduction of absorbed Au(III) to single crystalline flakes. Moreover, quantitative desorption of the CLE@50PEI-loaded metals was achieved in acidic thiourea solution.
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ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Xiang Li,Junyi Zhang,Bo Shi,Yawen Li,Yanan Wang,Kexiang Shuai,Yue Li,Gege Ming,Tao Song,Weihua Pei,Baoquan Sun
The electronic tattoo (e-tattoo), a cutting-edge wearable sensor technology adhered to human skin, has garnered significant attention for its potential in brain–computer interfaces (BCIs) and routine health monitoring. Conventionally, flexible substrates with adhesion force on dewy surfaces pursue seamless contact with skin, employing compact airtight substrates, hindering air circulation between skin and the surrounding environment, and compromising long-term wearing comfort. To address these challenges, we have developed a freestanding transparent e-tattoo featuring flexible serpentine mesh bridges with a unique full-breathable multilayer structure. The mesh e-tattoo demonstrates remarkable ductility and air permeability while maintaining robust electronic properties, even after significant mechanical deformation. Furthermore, it exhibits an impressive visible-light transmittance of up to 95%, coupled with a low sheet resistance of 0.268 Ω sq–1, ensuring both optical clarity and electrical efficiency. By increasing the number of menisci between the mesh e-tattoo and the skin, the total adhesion force increases due to the cumulative capillary-driven effect. We also successfully demonstrated high-quality bioelectric signal collections. In particular, the controlling virtual reality (VR) objects using electrooculogram (EOG) signals collected by mesh e-tattoos were achieved to demonstrate their potential for human–computer interactions (HCIs). This freestanding transparent e-tattoo with a fully breathable mesh structure represents a significant advancement in flexible electrodes for bioelectrical signal monitoring applications.
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APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2022
·Vol. 106
·期 23
·7805-7817
·
IF 5.56
Fu Linglong,Sun Mengning,Wen Weizhang,Dong Na,Li Defa
Abstract Small ubiquitin-like modifier (SUMO) tag is widely used to promote soluble expression of exogenous proteins, which can then be cleaved by ubiquitin-like protease 1 (Ulp1) to obtain interested protein. But the application of Ulp1 in large-scale recombinant protein production is limited by complicated purification procedures and high cost. In this study, we describe an efficient and simple method of extracellular production of Ulp1 403-621 using a leaky Escherichia coli BL21(DE3), engineered by deleting the peptidoglycan-associated outer membrane lipoprotein ( pal ) gene. Ulp1 403-621 was successfully leaked into extracellular supernatant by the BL21(DE3)-Δ pal strain after IPTG induction. The addition of 1% glycine increased the extracellular production of Ulp1 403-621 approximately four fold. Moreover, extracellular Ulp1 403-621 without purification had high activities for cleaving SUMO fusion proteins, and antimicrobial peptide pBD2 obtained after cleavage can inhibit the growth of Staphylococcus aureus . The specific activity of extracellular Ulp1 403-621 containing 1 mM EDTA and 8 mM DTT reached 2.0 × 10 6 U/L. Another commonly used protease, human rhinovirus 3C protease, was also successfully secreted by leaky E. coli strains. In conclusion, extracellular production of tool enzymes is an attractive way for producing large-scale active recombinant proteins at a lower cost for pharmaceutical, industrial, and biotechnological applications. Key points
• First report of extracellular production of Ulp1
403-621
in leaky Escherichia coli BL21(DE3) strain.
• One percent glycine addition into cultivation medium increased the extracellular production of Ulp1
403-621
approximately four fold.
• The specific activity of extracellular Ulp1
403-621
produced in this study reached 2.0 × 10
6
U/L.
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BIORESOURCE TECHNOLOGY
·
2017
·
IF 5.651
Yimin Li,Wenjie Yuan,Jiaoqi Gao,Chao Fan,Wenzhong Wu,Fengwu Bai
In this study, the optimum induction and reaction conditions, and fermentation process of producing Ala-Gln by E. coli Origami 2 overexpressing α-amino acid ester acyltransferase (OPA) were investigated. Besides, the Ala-Gln synthesis by OPA achieved the maximum molar yield of 94.7% and productivity of 1.89 g/L/min due to the extremely high enzyme activity. On this basis, repeated-cycle batch fermentation to produce Ala-Gln indicated that OPA could maintain high Ala-Gln yields and enzyme stabilities after several cell recycling. Consequently, the cost-efficient and environmentally friendly approach for Ala-Gln production by recycling OPA makes a great contribution to further industrial-scale applications.
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BRAZILIAN JOURNAL OF BIOLOGY
·
2021
·
IF 1.651
Huang Y.,Zhang N.-J.,Zhao Z.
As an important enzyme, xylanase is widely used in the food, pulp, and textile industry. Different applications of xylanase warrant specific conditions including temperature and pH. This study aimed to carry out sodium alginate beads as carrier to immobilize previous reported mutated xylanase from Neocallimastix patriciarum which expressed in E. coli, the activity of immobilization of mutated xylanase was elevated about 4% at pH 6 and 13% at 62 °C. Moreover, the immobilized mutated xylanase retained a greater proportion of its activity than the wide type in thermostability. These properties suggested that the immobilization of mutated xylanase has potential to apply in biobleaching industry.
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BIOMATERIALS
·
2019
·
IF 10.273
Yikun Yang,Xiaoyin Qiao,Ruiying Huang,Haoxiang Chen,Xuelei Shi,Jian Wang,Weihong Tan,Zhikai Tan
Drug-loaded implants have attracted considerable attention in cancer treatment due to their precise delivery of drugs into cancer tissues. Contrary to injected drug delivery, the application of drug-loaded implants remains underutilized given the requirement for a surgical operation. Nevertheless, drug-loaded implants have several advantages, including a reduction in frequency of drug administration, minimal systemic toxicity, and increased delivery efficacy. Herein, we developed a new, precise, drug delivery device for orthotopic breast cancer therapy able to suppress breast tumor growth and reduce pulmonary metastasis using combination chemotherapy. Poly-lactic-co-glycolic acid scaffolds were fabricated by 3D printing to immobilize 5-fluorouracil and NVP-BEZ235. The implantable scaffolds significantly reduced the required drug dosages and ensured curative drug levels near tumor sites for prolonged period, while drug exposure to normal tissues was minimized. Moreover, long-term drug release was achieved, potentially allowing one-off implantation and, thus, a major reduction in the frequency of drug administration. This drug-loaded scaffold has great potential in anti-tumor treatment, possibly paving the way for precise, effective, and harmless cancer therapy.
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Microbial Cell Factories
·
2021
·Vol. 20
·期 1
·1-13
·
IF 5.328
Zhang Yuyang,Chen Hongping,Zhang Yao,Yin Huifang,Zhou Chenyan,Wang Yan
Background Violaceins have attracted much attention as potential targets used in medicines, food additives, insecticides, cosmetics and textiles, but low productivity was the key factor to limit their large-scale applications. This work put forward a direct RBS engineering strategy to engineer the violacein biosynthetic gene cluster cloned from Chromobacterium violaceum ATCC 12,472 to efficiently improve the fermentation titers.Results Through four-rounds of engineering of the native RBSs within the violaceins biosynthetic operon vioABCDE, this work apparently broke through the rate-limiting steps of intermediates conversion, resulting in 2.41-fold improvement of violaceins production compared to the titers of the starting strain Escherichia coli BL21(DE3) (Vio12472). Furthermore, by optimizing the batch-fermentation parameters including temperature, concentration of IPTG inducer and fermentation time, the maximum yield of violaceins from (BCDE)m (tnaA−) reached 3269.7 µM at 2 mM tryptophan in the medium. Interestingly, rather than previous reported low temperature (20 ℃), we for the first time found the RBS engineered Escherichia coli strain (BCDE)m worked better at higher temperature (30 ℃ and 37 ℃), leading to a higher-level production of violaceins.Conclusion sTo our knowledge, this is the first time that a direct RBS engineering strategy is used for the biosynthesis of natural products, having the potential for a greater improvement of the product yields within tryptophan hyperproducers and simultaneously avoiding the costly low temperature cultivation for large-scale industrial production of violaciens. This direct RBS engineering strategy could also be easily and helpfully used in engineering the native RBSs of other larger and value-added natural product biosynthetic gene clusters by widely used site-specific mutagenesis methods represented by inverse PCR or CRISPR-Cas9 techniques to increase their fermentation titers in the future.
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Journal of Materials Chemistry C
·
2018
·Vol. 7
·期 3
·578-585
·
IF 5.976
Yibo Yang,Jize Liu,Jie Cao,Zehang Zhou,Xinxing Zhang
Despite the wide range of applications of flexible strain sensors, most of them use covalently crosslinked elastomers as a stretchable matrix, which may cause potential environmental problems via the disposal of worn-out devices. To mitigate this environmental threat, endowing sensors with self-repairability can be a simple and effective way to prolong their service lives. However, most current self-repairing sensors require complicated syntheses, in which hazardous solvents and reagents are always needed. Therefore, it remains challenging to develop an environment-friendly self-repairing sensor. Here, we report a sustainable self-repairing strain sensor based on naturally-derived supramolecular elastomer and green-synthesized silver nanofibers. This E-skin sensor features a high conductivity (≥104 S m−1), low percolation threshold (0.32%), high sensitivity (gauge factor ∼405.6) and desirable real-time (≤30 s) self-repairability after damage. This work may pave the way for the development of renewable, biocompatible and self-repairing next-generation wearable electronic devices.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Yachao Zhang,Yan Gao,Xueli Zhang,Huiming Wang,Tian Xia,Congcong Bian,Shuang Liang,Xiaozhi Tang,Xia Wang
The amplification of the electrochemical signal is crucial to improving the sensing performance of an immunosensor. In this study, a signal amplification strategy based on the co-catalysis of horseradish peroxidase (HRP) and nanoporous gold (NPG) was used to fabricate an electrochemical immunosensor for the determination of Hepatitis B e antigen (HBeAg). For the resulting immunosensor, HRP catalyzed catechol and thus served as a signal label of secondary antibody. NPG with unique properties was selected as a carrier for capture antibody immobilization. Further, NPG had an efficient electrocatalytic activity towards catechol (the substrate of HRP), which thus significantly enhanced the electrochemical signal produced by the HRP-labeled secondary antibody. Based on the co-catalysis of HRP and NPG, the immunosensor exhibited a good linear relationship between peak current density and HBeAg concentration in a wide HBeAg detection range from 1 pg mL −1 to 1 ng mL −1 with a high sensitivity of 23.51 μA mL ng −1 cm −2 as well as a low detection limit of 0.064 pg mL −1 (S/N = 3). Additionally, the immunosensor presented a strong anti-interference capability against human serum, human serum/dopamine, human serum/tryptophan, human serum/NO 2 − , human serum/D-glucose, and human serum/IgG. For the detection of HBeAg in human serum, the concentrations detected by the immunosensor were in good agreement with the standard concentrations that added in the human serum samples. These unique properties potentially make the proposed immunosensor a good choice for HBeAg detection.
研究领域:
生物医学工程
·
分析化学
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CELLULOSE
·
2022
·Vol. 29
·期 8
·4661-4671
·
IF 6.123
Li Zhen,Wang Feifei,Liu Lin,Liu Yixin,Liu Jikai,Chen Xinxin,Yao Juming
Flexible wearable electronic textiles (E-textiles) are predicted to apply in the intelligence management of physical training for automatic, intelligent, and scientific health management. Recently, printing electronic is one of the attractive processes for the large-scale manufacturing of patterned E-textiles. However, it is an enormous challenge that achieve sensing functionality without sacrificing the original performance of fabric. Herein, we proposed a new scalable aerodynamic assisted screen print technology to develop highly sensitive, breathable, and durable electronic textiles via the integration of graphene ink. Briefly, stable graphene ink was fabricated based on the exfoliation and dispersion of cellulose/PEDOT:PSS solution, and directly screen printed on spandex/cotton blend fabric (SCBF) with the assisting of airflow pressure. The as-prepared ink infiltrated and wrapped along the fiber surface by aerodynamic assisted screen printing instead of filling fabric inter-fibers, endowing the SCBF with conductivity (82.16 Ω/sq), flexibility and breathability. Consequently, the acquired E-textile exhibited superior air permeability (38.5 L·m 2 /s), washing durability (> 50 cycles), excellent sensitivity (GF = 36), strain sensing performance and stability (> 9000 cycles). Finally, a monitoring smart garment has been demonstrated to show the potential of a large-scale E-textile for physical training or health management. Graphical abstract
研究领域:
材料科学
·
电子工程
·
智能纺织品
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ANALYTICA CHIMICA ACTA
·
2026
·
IF 6.1
Yazhou Qin,Xuqing Wang,Mengze Fu,Jikai Mao,Yingsheng He,Jiye Wang
The incorporation of illegal drugs into e-cigarettes poses significant threats to both human health and societal safety. Therefore, the development of accurate and rapid detection methods for illicit drug additives in e-cigarettes is of significant importance. This study integrates deep learning with surface-enhanced Raman spectroscopy (SERS) to develop a highly sensitive detection method for nicotine and four etomidate isomers. First, Raman and SERS detection were performed on five drug standards. Furthermore, density functional theory calculations were employed to analyze the characteristic peak vibration modes of the various substances. Additionally, a liquid-liquid extraction pretreatment method for commercially available e-cigarettes was developed, facilitating the simultaneous detection of nicotine and etomidate isomers. Trace detection was successfully achieved for all five substances. Subsequently, an interpretable deep network model was constructed for the classification and identification of these substances, attaining an accuracy of 97.8%. To further enhance the model's transparency and interpretability, gradient-weighted class activation mapping (Grad-CAM) was utilized to reveal the critical SERS peaks necessary for molecular discrimination. This research provides robust technical support for the rapid on-site screening of illicit drugs in e-cigarettes.
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CARBOHYDRATE POLYMERS
·
2026
·
IF 13.2
Chenchen Shen,Weiwei Chen,Lin Guo,Yanrui Zou,Shuan Han,Ning Yu,Lin Gan,Jin Huang
Long-range piezoelectric transduction in flexible hydrogels is limited by the “trade-off” of conductive pathways between accelerating charge dissipation and suppressing dipole formation. Here, we introduce a surface-heterojunction strategy converting cellulose nanocrystals (CNCs) into conductive dipolar nanorods by deprotonation of poly(aminophenylboronic acid) (PABA) coating. Boronated ester bonding and hydrogen-bonds at CNCs/PABA interface collectively strengthen dipole moments while inducing n-type doping in PABA and thus enabling charge transport for 18.9-fold conductivity. Integrating molecular-level interfacial alignment of conjugated moieties, CNCs with higher aspect-ratio further promote percolation and reduce threshold by ~14%. The charge transport range in hydrogel is then elongated, which improves its conductivity by 7.0 times and yields robust piezo-electric/resistive dual-mode responsiveness. The hydrogen-bond network also improves adhesion, and imparts intrinsic sensitivity to temperature, salinity, and pH, for distinguishing complex motions and physiological events. This heterojunction-driven co-engineering of dipoles and conductivity offers a generalizable materials concept for advancing high-fidelity soft bioelectronic sensing.
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