ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Jing Feng,Lei Feng,Shuo Sun,Xujie Wang,Huitian Zhi,Ruhui Shang,Yingchao Yang,Luyao Wang,Zhengquan Yan,Lei Hu
Background NO 2 − and S 2− are two kinds of common toxic anions widely distributed in environmental water, soil and food products. Human beings have suffered a lot of diseases from intake of excessive NO 2 − or S 2− , i.e ., infantile methemoglobin , cancer and even to death. Although tremendous efforts have been afforded to monitor NO 2 − and S 2− , most were high instrument-depended with complex processing procedures. To keep food safety and to protect human health, it will be a huge challenge to develop a convenient and efficient way to monitor S 2− and NO 2 − in practice. Results A kind of folic acid capping Bi 3+ -doped Ag quantum dots (FA@Bi 3+ -Ag QDs) was developed for the first time by one-pot homogeneous reduced self-assembly. Not only did FA@Bi 3+ -Ag QDs possess intrinsic fluorescent property, it expressed synergistic peroxidase-like activity to catalyze the redox of 3,3′,5,5′-tetramethylbenzidine (TMB) and H 2 O 2 with K m / v max of 0.087 mM/6.61 × 10 −8 M s −1 and 6.42 mM/6.25 × 10 −7 M s −1 respectively. Interestingly, trace S 2− could exclusively alter its fluorescent property and peroxidase-like activity, exhibiting significant hypochromic and “turn-on” fluorescent effects. While trace NO 2 − could make FA@Bi 3+ -Ag QDs-TMB-H 2 O 2 system hyperchromic. Under the optimized conditions, FA@Bi 3+ -Ag QDs were applied for dual-mode recognition of S 2− and visual sensing of NO 2 − in real food samples with satisfactory recoveries, i.e ., 100.7–107.9 %/95.8–104.7 % and 97.2–104.8 % respectively. The synergistic enzyme-mimic mechanism of FA@Bi 3+ -Ag QDs and its selective response mechanisms to S 2− and NO 2 − were also proposed. Significance This represents the first nanozyme-based FA@Bi 3+ -Ag QDs system for dual-mode recognition of S 2− and visual sensing of NO 2 − , well meeting the basic requirement in drinking water set by WHO. It will offer a promising way for multi-mode monitoring of different pollution using the same nanozyme-based sensor.
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Food Bioscience
·
2024
·
IF 4.8
Shanting Lin,Xiao Hu,Shengjun Chen,Hui Huang,Xiaoshan Long,Zhenxing Li
The aims of this study were to investigate the effect of precise phosphorylation of Ser in GL-Hyp-GPSGEEGKR on its Fe 2+ chelating capacity, and the effects and mechanisms of folic acid coupling to GL-Hyp-GPS (PO4) GEEGKR on its resistance against degradation by gastrointestinal enzymes. The results demonstrated that GL-Hyp-GPS (PO4) GEEGKR exhibited significantly enhanced Fe 2+ chelating capability compared to GL-Hyp-GPSGEEGKR. This was attributed to the phosphorylation of Ser introducing more Fe 2+ chelating sites. Furthermore, the results of UV–Vis, FTIR, and 1 H NMR showed that folic acid has successfully coupled with GL-Hyp-GPS (PO4) GEEGKR to generate folic acid-GL-Hyp-GPS (PO4) GEEGKR. Folic acid-GL-Hyp-GPS (PO4) GEEGKR demonstrated superior resistance against degradation by gastrointestinal enzymes. This may be attributed to the coupling of folic acid altering the original zeta potential of the peptide and the steric hindrance effect of folic acid hindering the interaction between enzymes and peptide. These findings can provide valuable insights for the production of high Fe 2+ chelating peptides that resist gastrointestinal enzyme degradation.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Shu-Hua Han,Dan-Dan Huang,Zhang-Jian Cheng,Ai-Lin Liu,Yun Lei
Most known chemiluminescence (CL) systems are flash-type that generate weak luminescence and decline quickly after dozens of seconds, while the glow-type CL systems have stable emission for an extended period to achieve accurate quantitation. In this work, a long-term CL system based on hydrazine-hydrate (N 2 H 4 ·H 2 O) modified carbon quantum dots (N-CQDs) as a luminescent probe, with K 2 S 2 O 8 and H 2 O 2 as co-reactants, was proposed. The CL emission enhanced by H 2 O 2 increased 18-fold more than that of N-CQDs and K 2 S 2 O 8 direct reaction, and decayed by 5% of the maximum intensity over 700 s. In the reaction system, K 2 S 2 O 8 and H 2 O 2 co-reactants can promote each other to continuously generate corresponding radicals (•OH, O 2 •− , 1 O 2 ), which in turn trigger the CL emission of N-CQDs. This phenomenon was identified as the primary cause for the production of persistent CL. In addition, a stable and selective CL sensor based on the N-CQDs-K 2 S 2 O 8 -H 2 O 2 CL enhancing system was developed for ascorbic acid quantitation in the linear range from 0.1 to 10.0 mM with a detection limit of 0.036 mM. The method has been applied to the analysis of tablet samples and holds potential in pharmaceutical analysis field.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Fang Xie,Nian Liu,Xiaoqing Liu,Xiaofang Feng,Zhaoxing Yang,Zhijun He,Jie Cai
Selenium nanoparticles (SeNPs) exhibit significant potential in antitumor therapy. However, challenges such as aggregation and lack of targeting capability limit their application. Herein, we developed selenium-loaded octenyl succinic anhydride starch (OSAS) micelles functionalized with folic acid (FA) for targeted tumor delivery. The FA-OSAS-SeNPs were synthesized through self-assembly, incorporating SeNPs into FA-conjugated OSAS micelles. Fourier Transform Infrared (FTIR) spectroscopy and UV–visible spectrophotometry confirmed the successful synthesis of FA-OSAS-SeNPs. The nanoparticles exhibited an average size of 131.66 ± 7.88 nm and a zeta potential of −19.54 ± 0.33 mV, with encapsulation efficiency and drug loading capacity of approximately 87.28 % and 8.96 %, respectively. FA-OSAS-SeNPs demonstrated good stability across various conditions, including different dilution ratios, temperatures, pH levels, and ionic strengths. In vitro studies showed that FA-OSAS-SeNPs exhibited significant targeted inhibitory effects on cervical cancer (HeLa) cells and markedly increased intracellular ROS levels, inducing apoptosis. This study presents a novel and effective strategy for targeted SeNPs delivery systems in tumor therapy, offering a valuable reference for future development of nanomaterials for clinical applications in cancer treatment.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xiaohan Pan,Peng Wang,Jiahui Gu,Yuxiu Wang,Qingqing Li,Wenpeng Zhao,Shu Wang,Jingxin Gou,Xinyu Fan
Bladder instillation of chemo-therapeutic agents is common for bladder cancer (BC) treatment, however, due to the poor tissue selectivity of chemotherapeutic agents, this method suffers from bladder irritation or even chemical cystitis. Here, we designed a hydroxyethyl starch-based prodrug for epirubicin (EPI) using a pH-sensitive hydrazone linker and folate as the active targeting moiety (FA-HES-hyd-EPI) to achieve delivery selectivity. Prodrug micelles decorated with FA (FA-m), with diameter of 203.6 ± 7.1 nm and EPI loading of 5.80 ± 0.129 %, were prepared by self-assemble method. FA-m was found with improved cellular uptake and stronger cytotoxicity over EPI. An orthotopic BC model was established with FA receptor over-expression, and FA-m showed significantly higher accumulation at tissue and cell levels compared with the FA-free counterpart. Besides, the systemic exposure of FA-m was significantly lower than EPI (AUC 0-t : 28.11 ± 7.23 ng/mL/h versus 11.60 ± 4.27 ng/mL/h). More importantly, FA-m showed stronger in vivo tumor growth inhibition with average tumor weight of 0.26 ± 0.21 mg (EPI group: 1.695 ± 0.62 mg), and better safety according to body weight and HE staining data. Overall, the combination of active targeting and pH responsive prodrug strategies was a feasible way to achieve selective EPI delivery to BC via instillation that improved both therapeutic effect and safety.
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ACS Nano
·
2024
·
IF 15.8
Hui-Yue Zhao,Yuan-Qiang Chen,Xing-Yu Luo,Ming-Jie Cai,Jia-Yi Li,Xin-Yu Lin,Hao Zhang,Hong-Ming Ding,Guang-Liang Jiang,Yong Hu
Neutral nanomaterials functionalized with PEG or similar molecules have been popularly employed as nanomedicines. Compared to positive counterparts that are capable of harnessing the well-known proton sponge effect to facilitate their escape from lysosomes, it is yet unclear how neutral substances got their entry into the cytosol. In this study, by taking PEGylated, neutral Au nanospheres as an example, we systematically investigated their time-dependent translocation postuptake. Specifically, we harnessed dissipative particle dynamics simulations to uncover how nanospheres bypass lysosomal entrapment, wherein a mechanism termed as “squeezing-out” mode was discovered. We next conducted a comprehensive investigation on how nanomaterials implicate lysosomes in terms of integrity and functionality. By using single-molecule imaging, specific preservation of PEG-terminated with targeting moieties in lysosomes supports the “squeezing-out” mode as the mechanism underlying the lysosomal escape of nanomaterials. All evidence points out that such a process is benign to lysosomes, wherein the escape of nanomaterials proceeds at the expense of targeting moieties loss. Furthermore, we proved that by fine-tuning of the efficacy of nanomaterials escaping from lysosomes, modulation of distinct pathways and metabolic machinery can be achieved readily, thereby offering us a simple and robust tool to implicate cells.
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MOLECULAR PHARMACEUTICS
·
2024
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IF 4.5
Shaofang Ma,Yan Zhang,Zihan Zhu,Deping Wang,Xin Zhou,Jing Wang,Wei Bian,Xinjing Tang
The low cure rate and high mortality associated with cancer pose significant threats to human health. Photodynamic and photothermal therapies have emerged as promising treatment strategies for various types of cancers. In this study, we successfully synthesized a novel type of carbon dot (CD) using 1,2,4-aminobenzene and ethylenediamine as precursors. Surprisingly, these CDs exhibited outstanding nucleolus-targeting capabilities coupled with a remarkable photothermal effect. Through the integration of these nucleolus-targeting CDs with indocyanine green (ICG) and folic acid (FA), we created CDs-ICG-FA nanocomplexes suitable for combined photodynamic and photothermal therapy. In vitro experiments demonstrated that CDs-ICG-FA maintained a robust photothermal ability, achieving a conversion efficiency of up to 34.3%. Furthermore, CDs-ICG-FA generated abundant reactive oxygen species, effectively inducing cancer cell death and demonstrating its potential for photodynamic therapy. In MCF-7 cancer cells, CDs-ICG-FA exhibited a pronounced synergistic photothermal/photodynamic anticancer effect. Subsequent in vivo experiments in mice revealed that CDs-ICG-FA could selectively accumulate at tumor sites, significantly inhibiting tumor growth upon exposure to an 808 nm laser. These findings suggest that the developed nucleolus-targeting CDs-ICG-FA hold promising potential for cancer targeting and the application of combined photothermal/photodynamic therapy.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Yuanna Ning,Yongbo Wang,Shaojie Wang,Wencai Wang
Biothiol analysis is significant to health assessment and early detection of potential diseases. Considering practical requirements, simple and rapid identification and determination of biothiols are still a great challenge due to the similar structures. Fortunately, the recently emerging colorimetric sensor array technique makes such a task possible. Herein, one-component dual-mode sensor array consisting of carbon dots (CDs) and Ag nanoparticles (AgNPs) system was designed for identification and quantification of biothiols. The identification principle is based on the inner filter effect (IFE) and different binding constants. Due to the IFE between CDs and AgNPs, the fluorescence of CDs was quenched, but recovered again after addition of biothiols because of the binding of biothiols with AgNPs. Significantly, the fluorescence recovered in varying degree due to the different binding constants of biothiols to AgNPs. Meanwhile, the absorbance of the system decreased and the color of the solution deepened. Therefore, the CDs-AgNPs system with fluorescence and absorbance response was used as the single sensing unit and generated the cross-responsive signal for different biothiols. The sensor array achieved 100 % accuracy in identifying biothiols and biothiol mixtures. Moreover, the rapid quantification of biothiols in serum samples was also achieved by RGB-based smartphone colorimetry. The way to construct one component sensor array with dual mode signal outputs tremendously saves cost and time, providing a powerful tool for the identification of different biothiols. In addition, the rapid quantification of biothiols in serum samples based on RGB-based smartphone colorimetry demonstrated its powerful application prospects in disease diagnosis.
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Photonics
·
2024
·Vol. 11
·期 9
·820
·
IF 2.1
Ningyi Wang,Bingwei Liu,Xu Wu,Yan Peng
Vitamin C (VC) and Vitamin B9 (VB9) are essential micronutrients integral to numerous biological functions and critical for maintaining human health. The rapid detection of these vitamins is important for verifying nutritional supplements and aiding in clinical diagnoses. This study combined terahertz time-domain spectroscopy (THz-TDS) with metasurface technology to develop a fast, sensitive, and non-destructive detection method for VC and VB9. Firstly, we determined the characteristic absorption peaks and molecular vibration modes of VC and VB9 within the 0.5–4.0 THz range through quantum chemical calculation and THz-TDS measurement. Then, we designed and fabricated a metasurface biosensor to match its resonance peak with the communal peak of VC and VB9, enhancing the interaction between THz waves and these vitamins. Using this biosensor, we analyzed solutions with different concentrations of VC and VB9. An increase in vitamin concentrations resulted in frequency shifts in the THz resonance peak. Quantifiable relationships between frequency shifts and the vitamin concentrations were established. The detection limits achieved were 158.82 ng/µL for VC and 353.57 ng/µL for VB9, respectively. This method not only demonstrates high sensitivity but also simplifies the operational process, offering an innovative tool for applications in food safety monitoring and clinical diagnostics.
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APPLIED ORGANOMETALLIC CHEMISTRY
·
2024
·
IF 3.7
Zixuan Zhou,Yuhan Jiang,Xin Luo,Ying Zhang,Lei Wang,Yaguang Sun,Jingwei Liu,Mingchang Zhu,Jinchao Cao,Shuangyan Wu
Milk, owing to its nutritional value, is a staple commodity in daily life. Ensuring its integrity and authenticity is paramount for researchers and the food industry. To evaluate spoilage and adulteration of milk, we proposed a robust and reliable optical method to guarantee milk quality by utilizing riboflavin as an internal biomarker. By precisely controlling the content of Eu 3+ , Tb 3+ , and Gd 3+ ions, a two-dimensional ratiometric luminescence lanthanide coordination polymer (Ln-CP) with adjustable emission centers was synthesized for the detection of riboflavin (RF), leveraging a Förster resonance energy transfer (FRET) and photoinduced electron transfer (PET) process. This method demonstrates high sensitivity and selectivity towards RF and has been successfully applied to the detection of RF in both milk and calf serum.
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Journal of Analysis and Testing
·
2025
·
IF 7
Liang Yunyan,Zhou Xiaomeng,Liu Wenfeng,Shang Li
Rapid and effective differentiation of cancerous cells is essential for early diagnosis and treatment of tumors. Here, a multichannel sensor array is developed based on four surface-engineered gold-silver nanoclusters (AuAgNCs) to discriminate multiple cancerous and normal cells. Different binding affinities between various cells and AuAgNCs lead to changes in the intrinsic fluorescence of AuAgNCs as well as their peroxide-like activity. Based on the distinct response of dual fluorescence signals of four AuAgNCs, a multichannel sensor array is established, which is capable of accurately identifying both normal and cancerous cells, even cells of different subtypes and homologous cells from various individuals with high sensitivity. Moreover, this fluorescent clusterzyme-based sensor array enables quantitative detection of cancerous cells (e.g., HeLa cells) with good performance, which holds great potential as a promising platform for further cancer diagnosis.
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TALANTA
·
2018
·
IF 4.244
Weiwei Wang,Suqin Liu,Chengjie Li,Yan Wang,Chao Yan
A novel dual-target recognition sandwich strategy for selective capture and detection of MCF-7 breast cancer cells based on core-shell magnetic mesoporous silica (Fe 3 O 4 @nSiO 2 @mSiO 2 @apt) nanoparticles was developed. Fe 3 O 4 @nSiO 2 @mSiO 2 @apt nanoparticles, which were prepared by a layer-by-layer method and were used for the first time to capture cancer cells, have large surface areas, particularly accessible mesochannels, and good biocompatibility, enabling aptamers to be compactly anchored onto the surface of the core-shell magnetic nanoparticles . A mucin 1 protein (MUC1)-targeted Fe 3 O 4 @nSiO 2 @mSiO 2 @apt nanoparticle was used as an affinity magnetic isolate material to capture target MCF-7 cells selectively and to reduce interference through affinity interaction between the anti-MUC1 aptamer and the MUC1 protein over-expressed on the surface of the MCF-7 cells. Meanwhile, a folate receptor (FR)-targeted affinity fluorescent probe (FA-BSA-FITC) was developed by coupling folic acid and FITC to the surface of BSA, enabling high sensitivity, selective fluorescent labeling of FR over-expressed MCF-7 cells. A dual-target recognition sandwich assay was developed based on the MUC1-targeted magnetic nanoparticles and the FR-targeted fluorescent probes . Under optimum conditions, a quantitative assay of MCF-7 cells was achieved with a dynamic range of 10 2 –10 5 cells/mL (R 2 = 0.9991). This assay showed high specificity and sensitivity to the target MCF-7 cells. Finally, the proposed strategy could be extended to detect MCF-7 cells in human plasma and whole blood with a recovery range of 86.1–104.0% and a RSD range of 1.2–8.4%, respectively. This indicates that the dual-target recognition method developed in this research exhibits good selectivity , anti-interference capability, and reliability even in plasma and whole blood samples and is more suitable for complex samples than previous targeted assays. Therefore, the approach proposed here may have great potential for early breast cancer diagnosis.
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