JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2023
·
IF 9.9
Yingshuang Meng,Zhibo Qi,Zhonghua Li,Yuchao Niu,Mengyuan Wu,Zhijie Yuan,Gaohong He,Mingyang Yu,Xiaobin Jiang
Herein, a facile bionic research platform with fabricated hydrogel composite membrane (HCM) is constructed to uncover the effects of the main components of coffee’s metabolites on MSUM crystallization. Tailored and biosafety polyethylene glycol diacrylate/ N -isopropyl acrylamide (PEGDA/NIPAM) HCM allows the proper mass transfer of coffee’s metabolites and can well simulate the process of coffee’s metabolites acting in the joint system. With the validations of this platform, it is shown that chlorogenic acid (CGA) can hinder the MSUM crystals formation from 45 h (control group) to 122 h (2 mM CGA), which is the most likely reason that reduces the risk of gout after long-term coffee consumption. Molecular dynamics simulation further indicates that the high interaction energy ( E int ) between CGA and MSUM crystal surface and the high electronegativity of CGA both contribute to the restraint of MSUM crystal formation. In conclusion, the fabricated HCM, as the core functional materials of the research platform, presents the understanding of the interaction between coffee consumption and gout control.
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ANALYTICA CHIMICA ACTA
·
2023
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IF 6.2
Yanping Zhao,Xu Geng,Xiaoling Zhou,Li Xu,Shuai Li,Zhengqiang Li,Yi Guo,Chen Li
Pyruvate participates in diverse metabolic pathways in the body and is normally present in human blood at 40–120 μM, with concentrations outside this range associated with various diseases. Therefore, accurate and stable blood pyruvate level tests are necessary for effective disease detection. However, traditional analytical techniques require complicated instrumentation and are time consuming and expensive, prompting researchers to develop improved methods based on biosensors and bioassays. Here, we designed a highly stable bioelectrochemical pyruvate sensor affixed to a glassy carbon electrode (GCE). To maximize biosensor stability, 0.1 U of lactate dehydrogenase was affixed to the GCE using a sol-gel process, resulting in generation of Gel/LDH/GCE. Next, 2.0 mg/mL AuNPs-rGO was added to enhance current signal strength, resulting in generation of the bioelectrochemical sensor Gel/AuNPs-rGO/LDH/GCE. AuNPs-rGO synthesized in advance was verified as correct using transmission electron microscopy and UV–Vis, Fourier-transform infrared and X-ray photoelectron spectroscopy. Pyruvate detection conducted via differential pulse voltammetry in phosphate buffer (pH 7.4, 100 mM) at 37 °C for 1–4500 μM pyruvate provided detection sensitivity as high as 254.54 μA/mM/cm 2 . The reproducibility, regenerability and storage stability were analyzed with the relative standard deviation of 5 bioeletrochemical sensors detection was 4.60% and biosensor accuracy after 9 cycles was 92%, with accuracy remaining at 86% after 7 days. In the presence of D-glucose, citric acid , dopamine, uric acid and ascorbic acid , the Gel/AuNPs-rGO/LDH/GCE sensor exhibited excellent stability, high anti-interference ability and better performance than conventional spectroscopic methods for detection of pyruvate in artificial serum.
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IET Nanobiotechnology
·
2023
·Vol. 17
·期 4
·368-375
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IF 2.3
Jianmin Chen,Zemin He,Sijin Yu,Xiaozhen Cai,Danhong Zhu,Yanhua Lin
Ellagic acid (EA), which is widely distributed in many foods, has been found to possess inhibitory activity against xanthine oxidase (XO). However, there is ongoing debate about the difference in XO inhibitory activity between EA and allopurinol. Additionally, the inhibitory kinetics and mechanism of EA on XO are still unclear. Herein, the authors systematically studied the inhibitory effects of EA on XO. The authors’ findings showed that EA is a reversible inhibitor with mixed-type inhibition, and its inhibitory activity is weaker than allopurinol. Fluorescence quenching experiments suggested that the generation of EA-XO complex was exothermic and spontaneous. In silico analysis further confirmed that EA entered the XO catalytic centre. Furthermore, the authors verified the anti-hyperuricemia effect of EA in vivo. This study elucidates the inhibition kinetics and mechanism of EA on XO, and lays a theoretical foundation for the further development of drugs and functional foods containing EA for the treatment of hyperuricemia.
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RAPID COMMUNICATIONS IN MASS SPECTROMETRY
·
2023
·Vol. 37
·期 14
·e9529
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IF 2
Xin Zhang,Xiaoling Zang,Huanhuan Yang,Peng Jiao,Jie Zhang,Ni Song,Zhihua Lv
Rationale Thymoma is a rare malignant tumor but it is the most common primary tumor of the anterior mediastinum. The current imaging methods for thymoma screening suffer from false positive rate problems, and thymoma pathogenesis remains elusive. Study of thymoma metabolic characteristics could provide clues for improving the diagnosis and understanding the pathogenesis of thymoma. Methods Metabolic profiling of plasma from thymoma and thymic hyperplasia patients was performed using ultrahigh-performance liquid chromatography combined with high-resolution mass spectrometry in both positive and negative ionization modes. After pre- and post-processing, the dataset was divided into three age groups and statistical analysis was performed to select differential metabolites of thymoma. For feature identification, experimental tandem mass spectra were matched to those of databases and available chemical standards, and also manually annotated with plausible chemical structures to ensure high identification confidence. Results A total of 47 differential metabolites were identified in thymoma. Significantly higher levels of histidine, sphinganine 1-phosphate, lactic acid dimer, phenylacetylglutamine, LPC (18:3) and LPC (16:1), and significantly lower levels of phenylalanine, indole-3-propionic acid (IPA), hippuric acid and mesobilirubinogen were associated with thymoma. Tryptophan level in thymoma-associated myasthenia gravis (TAMG) was significantly lower than that of the MG(−) group. IPA and hippuric acid abundances exhibited increasing trends from indolent to aggressive thymoma. Conclusions Our study revealed aberrant aromatic amino acid metabolism and fatty acid oxidation might be associated with thymoma. The identified unique metabolic characteristics of thymoma may provide valuable information for study of the molecular mechanism of thymoma pathogenesis, and improvement of diagnosis and discovery of new therapeutic strategies for thymoma.
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Journal of Materials Chemistry C
·
2023
·Vol. 11
·期 22
·7272-7282
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IF 6.4
Linrong Zhang,Soukaina Khayour,Guozhang Ren,Shunhao He,Junjie Wang,Liuyingzi Yu,Yaxin Song,Chengcheng Zhu,Xing Kang,Yulong Zhang,Zhongyan Gong,Kun Gao,Jin Wang,Huixiang Sheng,Gang Lu,Hai-Dong Yu
Organic electrochemical transistors (OECTs) are promising for highly sensitive chemical and biological detections, because of their signal amplification, low operating voltage, solution compatibility, and high biocompatibility. However, OECTs, particularly the semiconductor layer, still need to be optimized for improving their output performances. Herein, by introducing Nafion, a proton penetrable polymer, into typical semiconductor layers of poly(3,4-ethylenedioxythiophene):polystyrenesulfonate (PEDOT:PSS) and poly(3-hexylthiophene) (P3HT), we realize a significant enhancement (up to 10-fold and 4-fold respectively) in transconductance of OECTs. The enhancements are attributed to the introduction of Nafion domains in the semiconductor channel, which increases the surface area of the semiconductor channel and promotes the ion doping/de-doping of the channel. In addition, the Nafion-including OECTs exhibit a fast response time of 20 milliseconds. Due to the improved transconductance and response time, the obtained OECT demonstrates sensitive detections of the pH value and glucose. This work provides a simple and effective method for improving the performance of OECTs, which will be promising in the sensitive and reliable detection of metabolites.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2021
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IF 4.098
Yaxin Zhao,Yuyu Ma,Yinhuan Li
A simple and feasible strategy was developed for the preparation of fluorescent covalent organic frameworks (COFs) TpPa-1@FL. The TpPa-1–1@FL was prepared via a self-assembly strategy by soaking non-fluorescent COFs TpPa-1 into strong fluorescent fluorescein (FL) solution. A chemiluminescence resonance energy transfer (CRET) system was constructed by the combination strong fluorescent TpPa-1@FL with TCPO-hydrogen peroxide (H 2 O 2 ) reaction. The chemiluminescence (CL) signal of the system was further improved by the addition of bovine serum albumin (BSA). The CRET system can determine H 2 O 2 with a linear range response from 5.0 µmol/L to 20.0 mmol/L and a detection limit of 1.1 µmol/L. The CRET system was further exploited for indirect detection of uric acid with coupling of uricase. A good linear relationship was obtained for uric acid in the 10.0–400.0 µmol/L concentration range with a detection limit of 3.8 µmol/L. The practicability of this method was assessed by the determination of uric acid in real samples of human serum and urine.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2021
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IF 4.464
Qian Du,Yanxin Liao,Nianfeng Shi,Shupei Sun,Xiaoming Liao,Guangfu Yin,Zhongbing Huang,Ximing Pu,Juan Wang
Metal-organic frameworks (MOFs) have great potential for electrochemical biosensors. In this paper, bimetallic MOFs were successfully in-situ grown on nickel foam (NF) through a facile one-pot hydrothermal treatment. X-ray diffraction, X-ray photoelectron spectroscope, Fourier transform infrared spectroscopy, energy dispersive spectrometer, and scanning electron microscopy were used to the structural and morphological characterization of bimetallic MOFs. Electrochemical measurement indicated that Cu 1 Co 2 -MOF/NF composite electrode processed a high sensitivity of 8304.4 μA mM −1 cm −2 , a low detection limit of 0.023 mM (S/N = 3). Moreover, it also showed good anti-interference ability toward ascorbic acid (AA), uric acid (UA), dopamine hydrochloride (DA) and sodium chloride (NaCl). These results suggest that Cu 1 Co 2 -MOF/NF is a promising electrode material for high-performance glucose sensing.
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BIOSENSORS & BIOELECTRONICS
·
2021
·
IF 10.618
Xiuguang Xing,Bangben Yao,Qian Wu,Ru Zhang,Li Yao,Jianguo Xu,Guangheng Gao,Wei Chen
A convenient, fast and non-invasive portable electrochemical uricometer (PUM) assisted with the uricase-packaged nanoflowers (NFs) was constructed for continually and accurately monitoring of uric acid (UA) in urine samples at random intervals in just 20 s. Only a small amount of urine (50 μL) was needed for each test. Electrochemical deposition was adopted to modify gold nanoparticles (AuNPs) on screen-printed carbon electrodes (SPCE) and uricase-inorganic hybrid NFs (UOx-NFs) induced by calcium ions (Ca 2+ ) were introduced for UA detection with expected specificity. Cyclic voltammetry (CV) (detection limit of 8.87 μM and liner range of 0–4 mM) and amperometry (detection limit of 0.82 μM and liner range of 0–5 mM) protocols were studied for UA detection, respectively. Finally, the uric acid in urine had be successfully continually monitored from volunteers with various dietary choosing, the results of which can be adopted as the effective evidence for uric acid control.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Huaxu Zhou,Xinyu Ma,Ajabkhan Sailjoi,Yanqi Zou,Xingyu Lin,Fei Yan,Bin Su,Jiyang Liu
Herein we report the simultaneous determination of serotonin (5-HT) and melatonin (MT) by using highly ordered and vertically-oriented mesoporous silica-nanochannel films (VMSF) on highly electrochemically reduced graphene oxide-carbon nanotubes (HErGO-CNT) composite substrate. Such VMSF/HErGO-CNT with compact 2D–2D layered structure is fabricated on indium tin oxide (ITO) electrodes via a two-step electrochemical process , namely the electrodeposition of ErGO-CNT film onto the ITO surface by cyclic voltammetry and subsequently growth of VMSF on the ErGO-CNT/ITO surface by electrochemically assisted self-assembly method, during which graphene oxide (GO) is subjected to the two-time electrochemical reduction to form HErGO. The CNT encased in the GO sheets served as electronic conducting wires, which not only can promote the electrochemical reduction of GO but also contribute to a certain degree of hydrophobicity , facilitating the controllable electrodeposition of ErGO-CNT composites on ITO under its safe use potential window. In addition, doping CNT is able to enlarge the layer gap between graphene sheets and thus improves the electroactive area and mass transfer of the nanocarbon composite substrate. Furthermore, the underlying HErGO-CNT acts as an efficient electroactive layer and the outer VMSF possesses electrostatic preconcentration and anti-fouling functions, synergistically realizing the direct electrochemical analysis of 5-HT and MT in two complex biological fluids (human whole blood and artificial cerebrospinal fluid).
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JOURNAL OF HAZARDOUS MATERIALS
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2021
·
IF 10.588
Weimeng Shi,Yan Cao,Xiaolong Chai,Qun Zhao,Yingxue Geng,Dan Liu,Senlin Tian
Microplastics (MPs), as pollutants of environmental concern, are correlated with increased risk of various respiratory diseases. Nevertheless, whether or not MPs have adverse influences on the interfacial properties of lung surfactant (LS), and its effect on the generation of reactive oxygen species are poorly understood. In the present study, natural LS extracted from porcine lungs was used to investigate the interaction with polystyrene as a representative MPs. The results showed that the phase behavior, surface tension, and membrane structure of the LS were altered in the presence of polystyrene. Adsorption experiments demonstrated that in the mixed system of polystyrene and LS (the main active ingredients are phospholipids and proteins), adsorption of phospholipid components by polystyrene was notably higher than that of proteins. Moreover, polystyrene can accelerate the conversion between ascorbic acid and deoxyascorbic acid, thereby producing hydrogen peroxide (HOOH) in simulated lung fluid (containing LS) and further giving rise to an increase in the content of hydroxyl radicals (•OH). This work provides new insight into the potential hazard of MPs in human respiratory system, which is helpful for deeply understanding the unfavorable physicochemical effects of MPs exposure and the role of inhaled MPs on lung health.
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CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Caicheng Long,Sicong Liu,Xiao Li,Jing Zhu,Lei Zhang,Taiping Qing,Peng Zhang,Bo Feng
In view of the increasing threat of chlortetracycline (CTC) in water to human health and the ecological environment, how to detect and remove CTC in wastewater simply and effectively remains an important and challenging task. Herein, a multifunctional platform for the simultaneous detection and degradation of CTC is developed by in-situ synthesis of carbon dots (CDs) in the two-dimensional (2D) WS 2 interface by covalent bonding. The as-prepared 2D WS 2 /CDs nanocomposites can not only be used as an intelligent fluorescence probe for CTC detection with good sensitivity (LOD = 7.5 nM) and selectivity but also has good photodegradation ability for CTC (degradation efficiency attained 85.58%). Moreover, the possible mechanisms for the detection and photodegradation of CTC by 2D WS 2 /CDs were also investigated by theoretical calculations, time-resolved fluorescence decay curves, and electron spin resonance (ESR) analyses. The exceptional photoelectric properties of 2D WS 2 /CDs are ascribed to the CDs bound onto the 2D WS 2 interface by covalent bonding forming heterojunctions, which effectively promote strong interfacial interactions between CDs and 2D WS 2 , thereby providing more effective charge transfer and enhancing the detection efficiency and photocatalytic activity. In addition, the possible degradation pathway for CTC was speculated via identification of intermediates and frontier electron density calculation. Overall, this study not only proves that the 2D WS 2 /CDs nanocomposites have great potential for the simultaneous detection and degradation of CTC in aquatic environments but also provides a novel idea for the efficient and stable design of multifunctional nanomaterials for environmental pollution monitoring and purification, which is of great significance to both practical applications and basic research.
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Frontiers in Chemistry
·
2021
·
IF 5.221
Hu Qin,Qin Jie,Wang Xiao-Feng,Ran Guang-Ying,Wang Qiang,Liu Guang-Xiang,Ma Jian-Ping,Ge Jing-Yuan,Wang Hai-Ying
A non-enzymatic electrochemical sensor for glucose detection is executed by using a conductive metal–organic framework (MOF) Cu-MOF, which is built from the 2,3,6,7,10,11-hexahydroxytriphenylene (HHTP) ligand and copper acetate by hydrothermal reaction. The Cu-MOF demonstrates superior electrocatalytic activity for glucose oxidation under alkaline pH conditions. As an excellent non-enzymatic sensor, the Cu-MOF grown on Cu foam (Cu-MOF/CF) displays an ultra-low detection limit of 0.076 μM through a wide concentration range (0.001–0.95 mM) and a strong sensitivity of 30,030 mA μM−1 cm−2. Overall, the Cu-MOF/CF exhibits a low detection limit, high selectivity, excellent stability, fast response time, and good practical application feasibility for glucose detection and can promote the development of MOF materials in the field of electrochemical sensors.
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