Acta Biomaterialia
·
2022
·
IF 10.633
Jiulong Zhang,Xiaoyan Sun,Mengdan Xu,Xiufeng Zhao,Chunrong Yang,Kexin Li,Fan Zhao,Haiyang Hu,Mingxi Qiao,Dawei Chen,Xiuli Zhao
Circumventing immune resistance and boosting immune response is the ultimate goal of cancer immunotherapy . Herein, we reported a tumor-associated macrophage (TAM) membrane-camouflaged nanodecoy containing a self-amplifying reactive oxygen species (ROS)-sensitive prodrug nanoparticle for specifically inducing immunogenic cell death (ICD) in combination with TAM depletion. A versatile ROS-cleavable camptothecin (CPT) prodrug (DCC) was synthesized through a thioacetal linker between CPT and the ROS generator cinnamaldehyde (CA), which could self-assemble into a uniform prodrug nanoparticle to realize a positive feedback loop of “ROS-triggered CA/CPT release and CA/CPT-mediated ROS generation.” This DCC was further modified with the TAM membrane (abbreviated as DCC@M2), which could not only target both primary tumors and lung metastasis nodules through VCAM-1/α 4 β 1 integrin interaction but also absorb CSF-1 secreted by tumor cells to disturb the interaction between TAMs and cancer cells. Our nanodecoy could effectively induce ICD cascade and deplete TAMs for priming tumor-specific effector T cell infiltration for antitumor immune response activation, which represents a versatile approach for cancer immunotherapy. Statement of significance A tumor-associated macrophage (TAM) membrane-camouflaged nanodecoy containing a self-amplifying reactive oxygen species (ROS)-sensitive prodrug nanoparticle was fabricated for the first time. This ROS-cleavable camptothecin (CPT)/cinnamaldehyde (CA) prodrug (DCC) could self-assemble into a uniform nanoparticle to realize the positive feedback loop of “ROS-triggered CA/CPT release and CA/CPT-mediated ROS generation.” After TAM membrane coating, this system (DCC@M2) could not only target both primary tumors and lung metastatic nodules but also scavenge CSF-1 secreted by tumor cells for TAM depletion for sufficient chemotherapy-sensitized immunotherapy.
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Food & Function
·
2022
·Vol. 13
·期 15
·8091-8106
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IF 6.317
Yanjing Ou,Mingdong Yan,Guanglin Gao,Wenjie Wang,Qiaoqiao Lu,Jiang Chen
Cinnamaldehyde (CA), the main active ingredient in cinnamon, has been proved to be a potential candidate for controlling inflammation; however, there has been little evidence demonstrating its role in alleviating periodontitis. The aim of this study was to investigate the effect of orally administered CA on ligature-induced periodontitis in mice and the administration of CA on the Porphyromonas gingivalis (Pg) supernatant-induced inflammatory responses of murine macrophages and human periodontal ligament cells (HPDLCs). In vivo experiments showed that the oral administration of CA significantly inhibited bone resorption, the accumulation of anaerobic bacteria and host immuno-inflammatory responses in ligature-induced periodontitis in mice. In vitro, CA inhibited the expression of Pg supernatant-induced IL6, IL8, TNFA and IL1B and reactive oxygen species in RAW 264.7 and HPDLCs, involving the inactivation of the NFKB signaling pathway, which was activated by the Pg supernatant. Also, the expression of adherent and chemotactic-related cytokines was inhibited by CA, accompanied with a reduction in adherent HPDLCs. Moreover, CA ameliorated the cellular senescence of HPDLCs induced by H2O2, together with a decrease in senescence-associated-β-galactosidase positive cells and decrease in the expression of P53, P21 and P16. Furthermore, CA promoted the osteogenic differentiation of HPDLCs with an increase in alkaline phosphatase expression and activity, formation of more mineralization nodules, and increased the expression of bone sialoprotein and osteopontin. Conclusions: Daily diet-added CA may be beneficial for oral health care, especially for the control of periodontic disease by suppressing the dysbiosis of biofilms and inhibiting the immunoinflammatory responses of migrated macrophages and local resident periodontal ligament cells to specific pathogen irritations.
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RSC Advances
·
2022
·Vol. 12
·期 36
·23215-23220
·
IF 4.036
Mingxin Luo,Bo Sun,Chen Zhou,Qingqing Pan,Yue Hou,Huan Zhang,Jing Sun,Chenyang Zou
In this research, a novel selective and sensitive fluorescent sensor for detecting Fe3+ was designed and synthesized; it revealed an obvious fluorescence quenching effect upon addition of Fe3+, and possessed the quantitative analysis ability on account of the formation of a 1 : 1 metal–ligand complex. Furthermore, the density functional theory calculations were utilized to study the molecular orbitals as well as the spatial structure. Simultaneously, the cell experiments and zebra fish experiments verified the application value of the sensor in the biological field.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 19
·11087
·
IF 6.208
Zhuangzhuang Li,Baoyan Ding,Mustafa R. K. Ali,Lizhen Zhao,Xiaoling Zang,Zhihua Lv
Abnormal tryptophan metabolism is linked to cancer and neurodegenerative diseases, and tryptophan metabolites have been reported as potential prostate cancer (PCa) biomarkers. However, little is known about the bioactivities of tryptophan metabolites on PCa cell growth. In this study, MTT and transwell assays were used to study the cytotoxicities of 13 major tryptophan metabolites on PCa and normal prostate epithelial cell lines. Ultraperformance liquid chromatography–high resolution mass spectrometry (UPLC–HRMS) was used to analyze metabolic changes in cells treated with tryptamine. Flow cytometry, confocal imaging, and Western blot were used to test the apoptosis induced by tryptamine. It was shown that tryptamine had obvious inhibitory effects on PCa cell lines PC-3 and LNCaP, stronger than those on the normal prostate cell line RWPE-1. Tryptamine was further shown to induce apoptosis and inhibit PC-3 cell migration. Metabolic changes including amino acid metabolism related to cell proliferation and metastasis were found in PC-3 cells treated with tryptamine. Furthermore, a PC-3 xenograft mouse model was used to study the effect of tryptamine in vivo. The intratumoral injection of tryptamine was demonstrated to significantly reduce the tumor growth and tumor sizes in vivo; however, intraperitoneal treatment resulted in increased tumor growth. Such dual effects in vivo advanced our understanding of the bioactivity of tryptamine in regulating prostate tumor development, in addition to its major role as a neuromodulator.Keywords:tryptamine;prostate cancer (PCa);cell proliferation;apoptosis;cell migration;xenograft model antitumor assays
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Arabian Journal of Chemistry
·
2022
·
IF 6.212
Jiaxin Li,Chen Zhou,Huan Zhang,Yue Hou,Qingqing Pan,Jing Sun,Xiao Li
In this work, a new highly selective and sensitive fluorescent sensor for detecting Cu 2+ was developed based on rhodamine fluorophore. It displayed strong fluorescence “turn-on” effect upon addition of Cu 2+ , and possessed the function of naked eye recognition. The fluorescence enhancement also enabled the sensor to quantitatively analyze Cu 2+ due to the formation of a stable 1:1 metal–ligand complex in a short time, and the complex possesses relatively good pH stability. In addition, the density functional theory calculations were adopted to investigate the molecular orbitals as well as the spatial structure. Simultaneously, the cell imaging and zebra fish experiments provided a broader application prospect in biological system.
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ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Yanju Chen,Hui Wu,Siwenjie Qian,Xiaoping Yu,Huan Chen,Jian Wu
Heavy metal lead accumulation in the environment pollutes the ecology systems and further threatens the human health. It is necessary to develop a sensitive method to detect it. Here, we propose a highly sensitive lead detection method by combining DNAzyme and CRISPR system. Once the lead ion is recognized, the substrate chain of DNAzyme is cleaved to produce single strand DNA . The produced single strand DNA can be detected by Cas protein/guide RNA complex and further trigger the collateral cleavage effect of CRISPR system, which can indiscriminately cut short single strand DNA reporters. By this way, the detection signals can be greatly amplified. This method can detect lead ions as low as 0.48 nM. The sensitivity is higher than the DNAzyme method. Furthermore, the portable 3D printing device is designed to observe the fluorescent signals so the end-point detection results can be visualized by the naked eyes. The entire detection process can avoid using bulky and expensive instruments, which can promote on-site lead ion detection.
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Analytical Methods
·
2021
·Vol. 13
·期 43
·5165-5172
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IF 2.896
Chunting Zhang,Kunhao Qin,Xiaodan Zheng,Qian Luo,Qi Zhang,Xiuling Ji,Yunlin Wei
Recent studies on preservation properties in the field of materials science suggest that a newly synthesized material can retain the biological properties of the raw material. Still, further study is necessary since these features critically influence research at the intersection of materials science and biology, and could provide a unique research direction for the synthesis of new materials. Thus, caffeic acid (CA) is used as a raw material to synthesize novel carbon quantum dots (CA-CDs) using a simple and rapid hydrothermal method. CA-CDs exhibit antiphage and antibacterial activities. Using three types of phages as models, it is confirmed that CA-CDs inhibit phage proliferation. The viral titres decline by 4, 5, or 6 orders of magnitude, and CA-CDs display potential universal antiphage ability; the mechanism suggests that the CA-CDs could change the protein structure of these phages and impact the phage adsorption stage leading to loss of infectivity. Meanwhile, the antibacterial activity is retained in CA-CDs, significantly inhibiting the growth of Gram-positive bacteria; this also reveals the preservation properties of CA-CDs. CA-CDs synthesized from CA, does not only expand the range of antiviral activity but also increases its many unique optical properties as a carbon dot material. This finding is meaningful in order to promote the development of new CDs with remarkable biological activity and acts as a basis for future scientific research on novel and alternative antiviral treatments.
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CONSTRUCTION AND BUILDING MATERIALS
·
2021
·
IF 6.141
Jingxuan Cai,Chunmei Zhang,Li Zeng,Hao Xu,Jia Wang,Kaiqiang Liu,Xiaowei Cheng
Encountering natural gas hydrate (NGH) is inevitable in the process of drilling and the production of oil and gas wells in deep-sea and shallow formations. The heat released by the cement slurry during the hydration process will cause the decomposition of gas hydrates, which will lead to annular channelling. Our purpose is to improve the cementing quality and ensure the safety of oil and gas production. In this paper, temperature control phase change microcapsules (PCMCs) with dodecanol (DA)/n-decanoic acid (CA) composite phase change materials (PCMs) as the core and active silica as the shell were prepared by a chemical precipitation method to store and release heat reversibly, to reduce the hydration heat of cement and improve the early strength. Fourier infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and energy spectrometry (EDS) were used to test and analyse the chemical structure and microscopic morphology of the synthesized temperature-controlled phase change microcapsules. Differential scanning calorimetry (DSC) was used to determine the phase change characteristics of the phase change microcapsules. The thermal stability of the phase change microcapsules was measured by a thermogravimetric analyser (TG). The application of PCMCs in a low-temperature cement slurry was also investigated. The results showed that the synthesized microcapsules had good thermal stability with a phase transformation temperature of 12.4 ℃ and enthalpy of 1.65 J/g. PCMCs can effectively control the hydration heat of cement and reduce the rise of hydration temperature by 6.06% to 11.79%. In addition, PCMCs can slowly release heat to promote the hydration of cement in the later strength development, and improve the early compressive strength of cement in the range of 5 ℃ to 10 ℃.
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INDUSTRIAL CROPS AND PRODUCTS
·
2021
·
IF 5.645
Ya-zhou Xu,Pan Fu,Song-lin Dai,Hai-bo Zhang,Liang-wu Bi,Jian-xin Jiang,Yu-xiang Chen
Vegetable oil-based vitrimers are innovative renewable polymers that show promising applications. However, their applications are limited by their poor mechanical properties and the need for the use of a catalyst in their preparation. In this study, the renewable tung oil underwent the methyl esterification reaction, Diels-Alder reaction, and epoxidation reaction to obtain TOTGE with terminal epoxy groups. TOTGE was cured with citric acid (CA) to obtain catalyst-free, self-healing, high mechanical strength fully bio-based TOTGE–CA vitrimers. Due to the high reactivity of their terminal epoxy groups, the vitrimer networks have a high density of crosslinks, and thus show good thermal stability and mechanical properties. The curing agent CA introduces a large number of hydroxyl (−OH) groups into the vitrimers and these form abundant hydrogen (H) bonds. The −OH groups in the vitrimers act as a catalyst, allowing topological network rearrangement of the materials in the absence of an external catalyst. Due to their dynamic ester and H bonds, the vitrimers exhibit good self-healing, recyclability, and shape memory properties, making them promising candidates for use as repairable and recyclable adhesives.
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Chinese Physics B
·
2021
·Vol. 30
·期 9
·097802
·
IF 1.494
Guohua Cao,Zhifei Wei,Yuehong Yin,Lige Fu,Yukun Liu,Shengli Qiu,Baoqing Zhang
In citric acid-based carbon dots, molecular fluorophore contributes greatly to the fluorescence emission. In this paper, the nitrogen and sulfur co-doped carbon dots (N,S-CDs) were prepared, and an independent sulfur source is selected to achieve the doping controllability. The influence of sulfur doping on the molecular fluorophore was systematically studied. The introduction of sulfur atoms may promote the formation of molecular fluorophore due to the increased nitrogen content in CDs. The addition surface states containing sulfur were produced, and S element exists as –SO3, and –SO4 groups. Appreciate ratio of nitrogen and sulfur sources can improve the fluorescence emission. The photoluminescence quantum yields (PLQY) is increased from 56.4% of the single N-doping CDs to 63.4% of double-doping CDs, which ascribes to the synergistic effect of molecular fluorophores and surface states. The sensitivity of fluorescence to pH response and various metal ions was also explored.
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JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
·
2021
·Vol. 110
·期 1
·220-228
·
IF 3.368
Xuan Chen,Xiaoyu Sui,Shuting Lu,Yanmei Qu,Tingting Liu,Ting Wang
Carbon dots (CDs) are nanomaterials with excellent photoluminescence property, usually used in the field of bioimaging tumor cells. However, its practical applicability in cancer therapeutics is limited by CDs' insensitive surface properties to complicated tumor microenvironment in vivo. Herein, a new type of innovative biomimetic nanoparticles has been formed with HeLa cell membranes (CM) and multifunctional CDs containing antitumor and bioimaging activities. The CDs are prepared by a facile one-step microwave-assisted procedure. Gallic acid is used as carbon resource and antitumor active molecule. Gelatin is treated as the nitrogen resource. Citric acid monohydrate is used as the auxiliary carbon source and the Hela CM is used for tumor targeting. A series of fluorescence analyses has proved its homotypic targeting and ability of diagnosis. Besides, in vitro and in vivo antitumor experiments further indicate their better antitumor efficiency. The findings show the totally new nanoparticles' feasibilities of dealing with the clinical therapy problems as well as applying for the integration of diagnosis and targeting therapy.
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Journal of Materials Chemistry C
·
2021
·Vol. 9
·期 36
·12255-12262
·
IF 7.393
Yi Han,Xiujian Zhao,Alberto Vomiero,Xiao Gong,Haiguang Zhao
Luminescent solar concentrators (LSCs) are large-scale sunlight collectors, consisting of fluorophores embedded in waveguides, which can concentrate part of the absorbed sunlight at the borders of the slab through wave-guided photoluminescence. Benefiting from their low-cost and semi-transparency, they exhibit great potential for building integrated photovoltaics. Among various types of fluorophores, carbon quantum dots (C-dots) have attracted great interest due to their relatively high quantum yield (QY), low-cost, non-toxic composition and simple synthetic methods. Unfortunately, most red-emitting C-dots with high QYs were synthesized using relatively toxic and expensive precursors. The C-dots exhibiting red-emission synthesized using sustainable precursors (e.g. citric acid) have QYs less than 20%. Here we synthesized the red-emitting C-dots produced by using citric acid and urea as precursors and N,N-diethylformamide as the solvent via a solvothermal reaction. The red C-dots have a broad absorption from 300–650 nm, with a QY as high as 40% in ethanol. In addition, the C-dots exhibited good biocompatibility, even for a C-dot concentration up to 1000 μg mL−1. The LSC (LSC area 100 cm2) based on red C-dots exhibited a solar-to-electricity power conversion efficiency (PCE) of 1.9% under natural sunlight illumination (35 mW cm−2). We combined red-emitting C-dots with green-emitting C-dots prepared via a vacuum heating approach. By using a tandem structure, composed of two slabs each incorporating a different C-dot type, the obtained PCE of the LSC based on the combination of red and green C-dots further increases up to 2.3% (under the same irradiance equal to 35 mW cm−2), which is comparable to the reported PCEs for the LSCs based on C-dots or other types of fluorophores. This work indicates that the red-emitting C-dots produced by low-cost and environmentally-friendly precursors exhibit great potential as building blocks for the environmentally compatible LSCs.
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