CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Shijie Yao,Yaru Hu,Jianfeng Ye,Jianhao Xie,Xuetao Zhao,Lingli Liu,Shuguang Lyu,Kuangfei Lin,Changzheng Cui
The super-resistant bacteria that produce the New Delhi metallo-β-lactamase 1 (NDM-1) enzyme are becoming a conceivably major worldwide clinical issue with serious consequences. Studying the disinfection and mechanism of super-resistant bacteria and their antibiotic resistance genes (ARGs) is crucial for controlling their spread in the water environment. In this study, the disinfection of the super-resistant bacterium Acinetobacter sp. CS-2 and its transferable ARGs (plasmid-encoded bla NDM-1 and bla OXA-58 ) by ultraviolet radiation (UV), peroxymonosulfate (PMS), and ultraviolet/peroxymonosulfate (UV/PMS) exposure were investigated. UV/PMS could completely inactivate CS-2 and control the photoreactivation risk, while bacteria that underwent UV and PMS treatment had survival ratios of 0.22% and 0.28%, respectively. The removal efficiencies of bla NDM-1 and bla OXA-58 ranged from 3.64 to 4.05 log, and the advantage of UV/PMS treatment on ARGs damage was further identified by gel electrophoresis analysis. The removal efficiency of bla NDM-1 was lower than that of bla OXA-58 , which was attributed to the low number of adjacent 5′-TT-3′ harbored by bla NDM-1 . The removal rate constants of the ARGs by UV treatment were significantly correlated with the number of adjacent base pairs (e.g., 5′-TT-3′, 5′-CT-3′, 5′-TC-3′ and 5′-CC-3′) and length, with R -values ranging from 0.9044 to 0.9512. Singlet oxygen ( 1 O 2 ) played a vital role in the reduction of bla NDM-1 and bla OXA-58 in the UV/PMS treatment. UV/PMS showed high removal efficiency under natural pH conditions and in the presence of various aqueous ions (e.g., Cl − , SO 4 2− and HCO 3 − ). Overall, this study provides new understandings and theoretical support for the risk control of super-resistant bacteria and plasmid-encoded ARGs.
研究领域:
环境科学
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微生物学
·
分子生物学
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DOI
高影响因子
INFLAMMATION RESEARCH
·
2025
·Vol. 74
·期 1
·1-19
·
IF 5.4
Weng Xiuhong,Wang Simin,Wang Qing,Wei Mingbo,Cheng Bo
Background Primary Sjögren’s syndrome (pSS) is an autoimmune disease characterized by xerostomia and autoimmune sialadenitis. Interferon-γ (IFN-γ) induces ferroptosis in salivary gland epithelial cells (SGECs), leading to salivary gland (SG) hypofunction. We previously demonstrated the beneficial effects of melatonin (MLT) in alleviating SG dysfunction and inflammation in a pSS animal model. However, the precise underlying mechanism remains unclear. Methods Female NOD/ltj and ICR mice were used as the pSS mouse model and control group, respectively. MLT was administered via intraperitoneal injection to NOD/ltj mice to detect its effect on ferroptosis in SGs. Primary human SGECs and A253 cells were treated with IFN-γand ferroptosis inducers, with or without MLT. Results Exogenous MLT alleviated pathological SG alterations and promoted saliva production through inhibiting SGEC ferroptosis. MLT inhibited SGEC ferroptosis induced by IFN-γ and ferroptosis inducers via nuclear factor erythroid 2-related factor 2/heme oxygenase-1/glutathione peroxidase 4 (NRF2/HO-1/GPX4) pathway activation. Moreover, MLT suppressed the nuclear factor-kappa B (NF-κB) pathway, which is triggered by ferroptosis in SGECs. Nevertheless, ML385-mediated NRF2 inhibition abrogated the antiferroptotic protective effects of MLT on SGECs. Conclusions MLT inhibits SGEC ferroptosis through NRF2/HO-1/GPX4 pathway activation and thus attenuates ferroptosis-triggered NF-κB activity. Melatonin represents a potential therapeutic approach for pSS owing to its capacity to regulate ferroptosis.
研究领域:
免疫学
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细胞生物学
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医学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF INFECTIOUS DISEASES
·
2008
·Vol. 197
·期 1
·117-125
·
IF 6.035
Michaëlsson Jakob,Long Brian R.,Loo Christopher P.,Lanier Lewis L.,Spotts Gerald,Hecht Frederick M.,Nixon Douglas F.
Natural killer (NK) cells are believed to play a role in human immunodeficiency virus type 1 (HIV-1) disease progression, and NK cell levels are reduced in individuals with chronic HIV-1 infection. Interleukin (IL)-2 therapy results in an expansion of CD4+ T cells as well as NK cells; however, little is known about the detailed effects of IL-2 therapy on NKcells in HIV-1 infection in general and in early infection in particular. Here, we investigated the effects of combined IL-2 therapy and antiretroviral therapy (ART) on the number, frequency, phenotype, and interferon (IFN)-γ production of NK cells in individuals with early HIV-1 infection. Patients randomized to receive combined ART and IL-2 therapy predominantly expanded CD56dim NK cells, and the expansion was greater than in patients randomized to receive ART alone. Importantly, NK cell receptor expression and IFN-γ production were maintained over time. This reconstitution ofNKcells may be useful in helping contain viremia if patients discontinue therapy or develop drug resistance.
研究领域:
免疫学
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病毒学
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传染病学
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Jinyi Wu,Shuyi Huang,Lei Tan,Yuling Li,Xiaotong Wu,Yong Liang
Dengue fever is caused by mosquito-transmitted dengue virus infection and continues to increase worldwide, threatening public health in tropical and subtropical regions. The primary difficulties in preventing a reduction of the medical burden of dengue fever lies in the lack of effective mosquito control, preventive dengue vaccines, and clinically effective antiviral drugs to treat dengue infections. Rapid and accurate diagnosis is crucial for proper patient care and effective control of epidemics. The present work proposes an alternative strategy for detecting the dengue virus nonstructural protein 1 (NS1) antigen in clinical serum samples by using ultrahigh-performance liquid chromatography tandem mass spectrometry (UHPLC–MS/MS) in combination with the molecularly imprinted polymers. Rather than the whole protein, the NS1 signature peptide is selected as a template for molecular imprinting and quantified as a stoichiometric readout of NS1. Three functional monomers with hydrophobic, positively charged, and negatively charged groups were synthesized by click reactions in terms of the signature peptide. These three functional monomers provide abundant recognition sites for the peptide, allowing the peptide template to be effectively imprinted during polymerization. The imprinting conditions were optimized, and the molecularly imprinted polymers were characterized and used for enriching the signature peptide from digested serum samples by solid-phase extraction and then detected by UHPLC–MS/MS. The proposed method is used to detect the dengue virus NS1 in clinical samples and holds significant promise for early confirmation of dengue virus infection.
研究领域:
医学诊断
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病毒学
·
分析化学
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DOI
高影响因子
Frontiers in Immunology
·
2020
·
IF 5.085
Ma Zhanchuan,Zhen Yu,Hu Cong,Yi Huanfa
Myeloid-derived suppressor cells (MDSC) play a crucial role in regulating the intestinal immune response during colitis. We previously revealed an essential role of MDSC in promoting TH17 cell polarization, which was found to be arginase-1 (Arg-1)-dependent; however, the underlying mechanism remains obscure. Here we report that percentage of MDSC decreased in ArgmyeKO mice during DSS-induced colitis. IL-17A levels reduced but IL-17F levels increased significantly in the colorectum of ArgmyeKO mice, leading to severe tissue damage and high risk of mortality rate. Activation of estrogen receptor (ESR) increased pSTAT3 level in MDSC and consequently led to elevated percentage of MDSC and more Arg-1 and inducible nitric oxide synthase expression in MDSC. Increased level of IL-17A and reduced level of IL-17F alleviated colitis in mice consequently. Together, these findings demonstrate a protective role of MDSC-derived Arg-1 during colitis after activates ESR/STAT3 signaling in MDSC. High level of Arg-1 favors accumulation of IL-17A, but reduced IL-17F expression in the colorectum of mice and ultimately leading to relief of colitis, indicating a potential clinical impact of MDSC-derived Arg-1 for controlling inflammatory bowel disease.
研究领域:
免疫学
·
分子生物学
·
消化病学
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2019
·
IF 8.456
Guanying Dong,Jingjing Zhang,Zheng Wang,Jing Wang,Peixia Zhao,Xingzhong Cao,Yatao Zhang
Polydopamine-modified additives have been thus far widely used in the mixed matrix membranes (MMMs) for gas separation. However, very few reports focus on the polydopamine alone and investigate its contribution to the gas separation performance. Herein, the polydopamine-derived submicrospheres (PDASS) were paired with polymers of intrinsic microporosity (PIM-1) to fabricate high-performance gas separation membranes, through which the effects of PDASS on gas permeability and CO2/N2 separation performance were systematically investigated. The addition of PDASS provides a 1.6-fold enhancement in CO2/N2 selectivity together with acceptable gas permeability as compared to the original polymeric membrane. Such enhanced separation behavior is supposed to stem from the densified membrane microstructure induced by the strong intermolecular interactions between PIM-1 and PDASS (i.e., charge transfer, π–π stacking, and hydrogen bonding). Importantly, the physical aging behavior, as judged by gas permeability, is retarded for PIM/PDASS membranes after 4 months of testing.
研究领域:
材料科学
·
化学工程
·
膜技术
·
气体分离
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DOI
高影响因子
Food and Bioprocess Technology
·
2026
·Vol. 19
·期 10
·496
·
IF 5.6
Gu Nixuan,Zhang Yingfan,Zhu Liang
Highly perishable fruit suffers postharvest losses from respiration, microbes, and microenvironmental shifts, which conventional packaging cannot dynamically address. This study developed dual-responsive active films via in situ hydrothermal synthesis of HKUST-1 within a chitosan (CS)/polyvinyl alcohol (PVA) matrix co-loaded with cinnamaldehyde (Cin). pH-responsiveness stems from CS amino protonation, while humidity sensitivity involves water-induced disruption of HKUST-1 carboxylate-Cu coordination. Structural properties (morphology, crystallinity, and bonding), mechanical strength, barrier function, and pH/humidity-responsive release kinetics were meticulously characterized. The acidic, high-humidity microenvironment generated during postharvest storage triggered the pH/humidity-dependent release of Cin, conferring potent antibacterial activity against Staphylococcus aureus ( S. aureus ). Preservation tests with Chilean blueberries demonstrated that the films prolonged shelf life by reducing decay, suppressing weight loss, and inhibiting microbial growth. This study establishes a novel strategy for microenvironment-responsive active packaging. The dual-release mechanism synchronizes preservative delivery with fruit spoilage signals, advancing intelligent preservation technologies for perishable foods.
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DOI
高影响因子
CATALYSIS LETTERS
·
2022
·Vol. 153
·期 9
·2813-2823
·
IF 2.936
Yang Jian,Li Xuemei,Zhang Jia,Tian Junying,Yao Xiaolan,Liu Hailong,Xia Chungu,Huang Zhiwei
Valuable 5-amino-1-pentanol (5-AP) was efficiently synthesized from biomass-derived dihydropyran over hydroxylapatite nanorod supported Ni catalysts (Ni-HAP), by coupling the in situ generation of 5-hydroxypentanal (5-HP, via the ring-opening tautomerization of 2-hydroxytetrahydropyran (2-HTHP)) and its subsequent reductive amination. The Ni-HAP catalyst with 10 wt% Ni loading exhibited the highest 5-AP yield than the other Ni-HAP catalysts with different Ni loadings and several commercial hydrogenation catalysts, including Ru/C, Pt/C and Pd/C, as well as Raney Ni. High Ni particles dispersion, high reducibility and acidic intensity were found to account for the superior catalytic performance of 10Ni-HAP. The effect of reaction parameters on the catalytic performance was investigated, and an excellent 5-AP yield of 92% was achieved under mild reaction conditions of 80 °C and 2 MPa H 2 . The stability of the Ni-HAP catalyst was studied using a continuous flow reactor, and the sintering of Ni nanoparticles was considered as the major reason for the decline in hydrogenation activity. Graphical Abstract The Ni-HAP catalyst exhibited high catalytic performance to synthesis 5-AP due to the high Ni particles dispersion, reducibility and acidic intensity.
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产品关联
DOI
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Zhengshan Gao,Xiang Liu,Chengli Zhang,Zhiyong Tang,Jun Chen,Chao Yu
A novel label-free electrochemical sensing strategy for detecting monocyte chemoattractant protein-1 (MCP-1) was developed by using single-walled carbon nanohorns (SWCNHs) functionalized with Pt nanoparticles (PtNPs-SWCNHs). After modification with the PtNPs-SWCNHs, the electrode could serve as an effective platform for antibody immobilization and electron transfer owing to the large surface area and excellent conductivity of PtNPs-SWCNHs. Moreover, the PtNPs-SWCNHs composite could provide an obvious electrochemical signal through amperometric i–t curve method, due to its high catalytic activity for the reduction of hydrogen peroxide (H 2 O 2 ). Under optimal conditions, the amperometric i-t curve signals had a strong linear relation with the logarithm of the MCP-1 concentration ranging from 0.06 pg mL -1 to 450 pg mL -1 , and the detection limit was 0.02 pg mL -1 . Furthermore, the proposed immunosensor could detect MCP-1 in serum samples with desirable results. Overall, this immunosensor may provide a new alternative strategy for rapid detection of MCP-1.
研究领域:
生物传感技术
·
医学检测
·
纳米材料科学
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DOI
高影响因子
ANALYST
·
2018
·Vol. 143
·期 11
·2501-2507
·
IF 3.864
Yong Liu,Xiaolin Xu,Haitang Yang,Ensheng Xu,Shuangshuang Wu,Wei Wei,Jin Chen
Poly(ADP-ribose) polymerase-1 (PARP-1) is a highly conserved nuclear enzyme, which binds tightly to damaged DNA and plays a key role in DNA repair, recombination, proliferation, and genomic stability. However, due to the poor electrochemical and optical activity of PARP-1 and its product PAR, only a few studies on its activity detection method have been reported. Herein, we report a simple and sensitive colorimetric strategy to monitor PARP-1 activity based on enzyme-initiated auto-PARylation-controlled aggregation of hemin-graphene nanocomposites (H-GNs). PARP, activated by dsDNA, catalyzed its substrate nicotinamide adenine dinucleotide (NAD+) to polymerize as a poly(ADP-ribose) polymer (PAR). PAR possesses several negative charges, and its charge density is twice that of a single-stranded DNA, which greatly impacts the dispersibility of H-GNs; due to their peroxidase-like catalytic activities, H-GNs can catalyze the chromogenic reaction of TMB and H2O2. As a result, in the presence of different PARP-1 activities, the supernatant of the corresponding solution contained different amounts of dispersed H-GNs and showed different colors after the chromogenic reaction that could be discerned easily by the absorbance or the color changes of the solution. The method was simple, sensitive, and reliable. The proposed method displays a linear range from 0.05 to 1 U with a detection limit of 0.03 U. In addition, this new method has been successfully applied to detect PARP-1 activity in human serum and different cancer cells and evaluate PARP-1 inhibitors.
研究领域:
生物化学
·
分子生物学
·
癌症研究
·
纳米技术
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DOI
PubMed
高影响因子
IONICS
·
2016
·Vol. 22
·期 10
·1947-1955
·
IF 2.119
Zhuo Kelei,Ma Xueli,Chen Yujuan,Wang Congyue,Li Aoqi,Yan Changling
The molecular imprinting technique is powerful to prepare functional materials with molecular recognition properties. In this work, a potentiometric sensor was fabricated by dispersing molecularly imprinted polymers (MIPs) into plasticized PVC matrix and used for the determination of 1-hexyl-3-methylimidazolium cation ([C 6 mim] + ) in aqueous solution. The MIPs were synthesized by precipitation polymerization using 1-hexyl-3-methylimidazolium chloride ([C 6 mim]Cl) as the template molecule, methacrylic acid (MAA) and ethylene glycol dimethacrylat (EGDMA) as the functional monomers, and EGDMA also as the cross-linking agent. The as-prepared electrode exhibited a Nernstian response (58.87 ± 0.3 mV per decade) to [C 6 mim] + in a concentration range from 1.0 × 10 −6 to 0.1 mol kg −1 with a low detection limit of 2.8 × 10 −7 mol kg −1 , high selectivity, and little pH influence. The as-prepared electrode was used for the detection of the [C 6 mim] + in distilled water, tap water, and river water with a good recovery. It was also successfully applied in the determination of mean activity coefficients of [C 6 mim]Br in fructose + water systems based on the potentiometric method at 298.15 K.
研究领域:
分析化学
·
材料科学
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DOI
高影响因子
JOURNAL OF CHEMICAL SCIENCES
·
2020
·Vol. 132
·期 1
·1-7
·
IF 1.406
DU ZHONGLIN,LI FENRUI,LI LI,LI RAN
Abstract Microwave-assisted one-pot quick synthesis to 1-monosubstituted 1,2,3-triazoles was achieved with good to excellent yields using the widely available arylboronic acids, sodium azide and 3-butyn-2-ols within 15 min. This method features high efficient and facile as organic azides, acetylene gas and harsh conditions were avoided. Graphic abstract Microwave-assisted one-pot quick synthesis to 1-monosubstituted 1,2,3-triazoles was achieved with good to excellent yields using the widely available arylboronic acids, sodium azide and 3-butyn-2-ols within 15 min.
研究领域:
有机化学
·
药物化学
·
合成方法学
·
绿色化学
·
杂环化学
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DOI
高影响因子
危险品化学品经营许可证（带存储）