BIOMATERIALS
·
2025
·
IF 12.9
Rui Hu,Xin Jiang,Lijie Zhu,Rui Meng,Rongbo Yang,Wenjie Sun,Zhenzhou Zhao,Yuehua Lyu,Ruoyuan Huang,Fei Xue,Mengke Shi,Zaigang Zhou,Jianliang Shen,Congying Xie
Currently, certain lung cancer patients exhibit resistance to radiotherapy due to reduced DNA damage under hypoxic conditions and the cytoprotective and immune-resistance effect caused by increased programmed death ligand-1 (PD-L1) and Cyclooxygenase 2 (COX-2) expression after radiotherapy. At present, existing nanoparticles or drugs could hardly effectively, and easily address these obstacles faced by highly effective radiotherapy simultaneously, especially the simultaneous depression of PD-L1 and COX-2. In this study, it is newly proved that some typical nitric oxide (NO) gas donors could co-inhibit PD-L1 and COX-2 expression, revealing the possible not fully proven role of NO in reversing tumor immunotherapy resistance. Then, to realize selective NO generation in tumors, a simple tumor glutathione (GSH) responsive NO gas nanogenerator named SAB-NO nanoparticles was designed and prepared, which was composed of the NO donor Isoamyl Nitrite conjugated with serum albumin (SAB). By doing this, SAB-NO nanoparticles more effectively sensitized radiotherapy through breaking the cytoprotective effects faced by radiotherapy in vitro by generating more DNA damage through reversing tumor hypoxia and impairing the DNA damage repair process through decreasing PD-L1 expression. Then, the combination therapy of SAB-NO nanoparticles and radiotherapy effectively transformed cold tumors into hot ones through avoiding some potential immune-resistance effects induced by radiotherapy treatment alone through PD-L1 and COX-2 co-inhibition. In conclusion, the combined treatment of radiotherapy and SAB-NO nanoparticles finally almost completely suppressed the growth of lung tumors, revealing the novel role of NO donors in sensitizing tumor immunotherapy by avoiding the potential cytoprotective and immune-resistance effects.
研究领域:
肿瘤学
·
免疫学
·
药剂学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Xiaona Sun,Chunqing Ai,Chengrong Wen,Haoran Peng,Jingfeng Yang,Yuna Cui,Shuang Song
Fucoidan is a highly sulfated polysaccharide with a wide range of bioactivities, including anti-pathogenic activity. However, the relationship between structure and activity of fucoidan in inhibiting pathogen infections remains unclear. Here, different-molecular-weight fucoidans were prepared by photocatalytic degradation followed by membrane ultrafiltration, and their chemical structures and anti-pathogenic microbiota activity were compared. Results showed that photocatalytic degradation could effectively degrade fucoidan while its structure block and sulfate groups were not destroyed obviously. Fucoidan (90.8 kDa) of 5 mg/mL could inhibit the growth of S. aureus , S. typhimurium and E. coli , but its degradation products, Dfuc1 (19.2 kDa) and Dfuc2 (5.5 kDa), demonstrated lower inhibitory effect. In addition, compared to Dfuc1 and Dfuc2, fucoidan showed stronger capability to prevent the adhesion of S. aureus , L. monocytogenes , V. parahaemolyticus and S. typhimurium to HT-29 cells. Moreover, the inhibitory effect against SARS-CoV-2 and the binding activity to S protein were also positively correlated to molecular weight. These results indicate that natural fucoidan with higher molecular weight are more effective to inhibit these pathogenic bacteria and SARS-CoV-2, providing a better understanding of the relationship between structure and activity of fucoidan against pathogenic microbiota.
研究领域:
微生物学
·
生物化学
·
病毒学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2021
·
IF 4.464
Mingguang Ma,Jing Lu,Ling Wang,Xia Qiu,Tingting Xu,Sufan Wang,Yinggui Zhu
In this work, we first reported the electrochemiluminescence (ECL) properties of 2-hydroxynicotinic acid (2-HNA) with 0.09 M K 2 S 2 O 8 in 0.5 M H 3 BO 3 – Na 2 B 4 O 7 buffer solution. The effects of the type of co-reactant, the concentration of K 2 S 2 O 8 and the pH of the buffer solution on the ECL intensity of this system were investigated. And the possible mechanism of 2-HNA/ K 2 S 2 O 8 system has been speculated by fluorescence spectrum and ECL spectrum. Besides, we found that phloxine B (PB) can significantly enhance the ECL intensity of the 2-HNA/ K 2 S 2 O 8 system. It is speculated that an electrochemiluminescence resonance energy transfer (ECL-RET) process may occur between 2-HNA and PB, so that PB enhanced the ECL of the 2-HNA system. Combining the ultraviolet spectrum and density functional theory (DFT) calculations further proved the rationality of this speculation. Based on this, a new method of ECL detection of PB with high sensitivity and high selectivity was established.
研究领域:
分析化学
·
电化学
✓ 已验证
DOI
高影响因子
Catalysis Science & Technology
·
2020
·Vol. 10
·期 13
·4412-4423
·
IF 5.721
Qiang Liu,Zhenbo Zhao,Masahiko Arai,Chao Zhang,Ke Liu,Ruhui Shi,Peixuan Wu,Zhuangqing Wang,Weiwei Lin,Haiyang Cheng,Fengyu Zhao
The transformation of γ-valerolactone (GVL) into 1,4-pentanediol (1,4-PDO) and 2-methyltetra-hydrofuran (2-MTHF) in the presence of H2, one of the useful biomass conversion and utilization processes, was investigated with monometallic Cu/Al2O3 and bimetallic ZnCu/Al2O3 catalysts. A 10 wt% Cu-loaded monometallic catalyst produced 1,4-PDO and 2-MTHF in comparable quantities at a medium conversion (∼50%). When Zn was added in a range of Zn/Cu molar ratios of up to 2, in contrast, the catalysts yielded 1,4-PDO in a high selectivity of about 97% at low and high conversion levels. In addition, the 1,4-PDO selectivity over the ZnCu/Al2O3 catalysts remained almost unchanged during recycled runs. That is, the addition of Zn to Cu/Al2O3 switched the product selectivity and improved the catalyst stability and reusability. Furthermore, the physicochemical properties of the catalysts were characterized by several methods including XRD, TEM, TPR, XPS, FTIR of adsorbed pyridine, and so on. On the basis of those results, the relationships between the catalytic performance (activity, selectivity, and reusability) and the catalyst structural features were discussed.
研究领域:
催化化学
·
生物质转化
·
材料科学
✓ 已验证
DOI
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 14
·3396
·
IF 4.2
Zhengyang Wang,Shuting Wang,Yuexing Zhang,Mingliang Ma
A series of fluorescent molecules with 1,1-dimethylnaphthalene-2(1H)-one as the core were synthesized to overcome aggregation quenching and emit bright green fluorescence. The low molecular weight of these molecules led to them to smoothly pass through the cell membrane and penetrate deep into the nucleus to emit the corresponding fluorescence. Among them, NC-4-Br and NC-5-3O have good optical and in vitro properties and showed potential for use as fluorescent probes.
研究领域:
生物化学
·
分子生物学
·
医学成像
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2024
·
IF 5.3
Ying Yang,Jixiang Hu,Hui Wang,Lei Qiao,Yan Wang,Wenhua Tong,Liming Zhao
Eugenol (EGL) and 2-methylpyrazine (2-MTP) are common food additives that are used as flavors in a variety of foods. To study the effect of food additives on human serum albumin (HSA), it is necessary to deeply study the interaction of EGL and 2-MTP with HSA. Fluorescence, UV–vis absorption, circular dichroism (CD) and computational techniques showed EGL and 2-MTP bind statically to HSA, inducing conformational changes of HSA. We calculated the quenching constants (k sv ), binding constants (k q ), binding distances (r) and thermodynamic constants (ΔH 0 , ΔS 0 , ΔG 0 ) at four different temperatures. Based on thermodynamic analysis and molecular dynamics (MD) simulations, the main forces of 2-MTP-HSA and EGL-HSA systems are hydrophobic interaction and π-π accumulation, respectively. In addition, the interaction alters the physiological activity of HSA, esterase activity and free radical scavenging ability. This study provides valuable insights into the molecular interactions of food additive compounds EGL and 2-MTP with HSA, the findings provide theoretical reference for the development of new food additives and drugs in the future.
研究领域:
生物化学
·
食品科学
·
药物开发
✓ 已验证
DOI
高影响因子
BIOCHEMICAL SYSTEMATICS AND ECOLOGY
·
2025
·
IF 2
Li-Juan Hu,Xian-Sheng Ye,Kuan Lin,Jie Gao,De-Sen Yang,Guo-Ping Gan,Xi-Ji Shu,Wei Liu,You-Hua Yang
One previously undescribed 2-arylbenzofuran derivative ( 1 ), three stilbenes ( 2 - 4 ), three acetophenone glycosides ( 5 – 7 ), three phenylpropanoids ( 8 – 10 ), and eight flavonoids ( 11 – 18 ) were isolated from the rhizome of Smilax china L. in this study. NMR spectra elucidated their structures and were compared with previously reported data. Compounds 1 , 5 – 7 , 10 , and 18 were identified for the first time in Smilax china L. The cytotoxicity of the isolated compounds was detected on HeLa cells by the CCK-8 method, and compound 4 was found to possess the highest cytotoxic activity with an IC 50 value of 34.81 ± 1.50 μg/mL. Further studies revealed that the cytotoxic effect of compound 4 on HeLa cells was related to apoptosis and the cell cycle. In addition, the chemotaxonomic significance of the isolated compounds was discussed.
研究领域:
植物化学
·
药理学
·
细胞生物学
·
化学分类学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF COSMOLOGY AND ASTROPARTICLE PHYSICS
·
2018
·Vol. 2018
·期 09
·023
·
IF 5.126
Basabendu Barman,Subhaditya Bhattacharya,Mohammadreza Zakeri
An SU(2)N extension (N stands for neutral) of the Standard Model (SM) is proposed with an additional U(1)=S' global symmetry, which stabilizes the lightest of the vector boson (X,) as dark matter (DM) through unbroken S=T3N+S'. The field content of the model is motivated to address neutrino mass generation, a possible unification to SU(7), along with spontaneous symmetry breaking of SU(2)N resulting in massive gauge bosons. None of the SM particles are charged under SU(2)N and therefore X, do not have a direct coupling to the visible sector besides a Higgs portal, which is tiny to avoid any conflict with Higgs data. We show that, a large kinematic region of this model allows the neutral component of SU(2)N scalar triplet and heavy neutrinos introduced here to become additional DM components. In this paper we explore the viability of such multipartite DM parameter space, including non-zero DM-DM interactions, to comply with relic density and direct search constraints. We also demonstrate that the model may yield hadronically quiet single lepton and two lepton signatures with missing energy at the Large Hadron Collider (LHC) that can be accessed with high luminosity.
研究领域:
粒子物理学
·
天体物理学
✓ 已验证
DOI
高影响因子
FASEB JOURNAL
·
2025
·Vol. 39
·期 11
·e70691
·
IF 4.2
Jianfeng Dong,Wen Chen,Shujuan Chang,Nana Liu,Wei Zhu,Li Ma,Jing Qiao,Yuhang Wang,Zhiyong Mao,Jiuhong Kang
Infants born with fetal growth restriction (FGR) are at increased risk of cognitive impairment in later life, yet the underlying mechanisms remain poorly understood, hampering clinical treatment development. Utilizing a FGR mouse model induced by prenatal dexamethasone overexposure, we observed impaired hippocampal dendritic maturation, synaptic plasticity, and hippocampal‐dependent learning and memory in FGR mice. FGR induces DNA hypomethylation in the hippocampal H19/Igf2 imprinted control region, resulting in reduced expression of Igf2, a key metabolic modulator previously implicated in FGR‐related placental and fetal growth. Using a neuron‐specific knockout mouse model, we validated the necessity of Igf2 for dendritic maturation and synaptic plasticity. Importantly, overexpression of Igf2 in the hippocampus of FGR mice improved synaptic plasticity and enhanced learning and memory abilities. Furthermore, running exercise ameliorated DNA hypomethylation driving Igf2 expression levels and counteracted synaptic and cognitive deficits in FGR mice through hippocampal Igf2. These findings unveil a molecular mechanism linking exercise‐induced epigenetic changes in the imprinting gene Igf2 to enhanced synaptic plasticity, ultimately leading to improved cognition in FGR offspring. Our results identify Igf2 as a potential mediator bridging metabolic dysfunction and cognitive impairments in FGR offspring and also propose that exercise represents a promising nondrug intervention for FGR‐related cognitive disorders. Fetal growth restriction (FGR) induces hippocampal DNA hypomethylation and loss of Igf2, contributing to impaired synaptic plasticity and cognitive deficits in offspring. Exercise restores Igf2 levels and rescues these impairments, suggesting a potential therapeutic strategy for FGR‐related cognitive dysfunction.
研究领域:
神经科学
·
分子生物学
·
医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RENEWABLE ENERGY
·
2021
·
IF 8.001
Hongyou Wan,Fuzhen Wang,Yuhan Chen,Zisheng Zhao,Guangyi Zhang,Ming Dou,Binghan Xue
In this study, nano-Fe 3 O 4 supporting on granular activated carbon (NFGAC) were prepared, characterized and used as an individual additive to promote the anaerobic digestion of azo dye Reactive Red 2 (RR2) wastewater. Results showed that organics removal and methane production were effectively promoted with the addition of NFGAC. Compared with the control reactor, the RR2 removal efficiency increased by 117.9% and the methane production increased by 167.2% in the 0.18-NFGAC added reactor when sole RR2 used as the substrates. Further analysis indicated that during the RR2 anaerobic digestion process the electron transfer system (ETS) activity was effectively improved with the addition of NFGAC. One of the possible mechanism was that the loading nano-Fe 3 O 4 on the surface of GAC could increase the number of functional groups in NFGAC, which were beneficial to enhancing the electron accepting capacities (EAC) and electron donating capacities (EDC). Microbial community analysis showed that the bacteria ( Halomonas , Pseudomonas and Bacillus ) relevant to RR2 removal were enriched in NFGAC added reactors, which played an important role during RR2 removal process. In addition, the addition of NFGAC could promote potential direct interspecies electron transfer (DIET) between Pseudomonas and Methanosarcina.
研究领域:
环境科学
·
微生物学
✓ 已验证
DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2025
·
IF 3.1
Minlu Cheng,Xianjing Li,Ya Li,Yiya Wang,Wenjia Li,Shuai Wang,Chang Shu,Qinxin Song,Li Ding
Recently, the approval of Trop-2 targeted antibody drug conjugate (ADC) has changed the dilemma of patients with advanced triple-negative breast cancer who rely on chemotherapeutics to improve their survival. FDA018, an ADC consisting of an anti-Trop-2 antibody conjugated with a topoisomerase inhibitor SN-38 via an acid-cleavable linker, is currently being investigated in clinical trials. Based on the urgent demand to evaluate the clinical pharmacokinetics of FDA018, ligand binding assays (LBAs) for the determination of SN-38-conjugated antibody and total antibody and LC-MS/MS methods for the determination of the free SN-38, its metabolite SN-38G and total SN-38 were developed. The comparability and DAR sensitivity evaluation of the ELISA strategies for SN-38-conjugated antibody and total antibody were emphasized. The sensitivity of the LC-MS/MS method for the simultaneous determination of SN-38 and SN-38G reached 0.500 ng/mL and 0.250 ng/mL, respectively. An effective solution has been proposed for the optical instability of the cleavable linker of FDA018 during the pretreatment process of biological samples. The established bioanalytical methods were comprehensively validated and the results satisfied the acceptable criteria of ICH M10. The validated bioanalytical methods have been applied to the single-dose pharmacokinetic study of FDA018 in patients with Trop-2-positive malignant tumors successfully, and the pharmacokinetic profiles of FDA018 and its constituent components were investigated.
研究领域:
肿瘤学
·
药物分析
·
临床药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 4
·1-8
·
IF 6.232
Li Jingxian,Shan Xueling,Jiang Ding,Wang Wenchang,Chen Zhidong
Ultrasensitive and selective determination of bisphenol A (BPA) was achieved by a solid-state electrochemiluminescence (ECL) aptasensor. The Ti-based MOFs (MIL-125, MIL stands for Materials of Institute Lavoisier) have porous structure and large specific surface area. They were used as a carrier for Ru(bpy) 3 2+ by a hydrothermal synthesis method. The Ru(bpy) 3 2+ encapsulated MIL-125 (Ru(bpy) 3 2+ @MIL-125) was dropped on a glass carbon electrode (GCE) and used as working electrode. After that, the thiol-based aptamer of BPA was used to modify the working electrode by Ti–S bond. As a result of the specific binding affinity between aptamer and BPA, the ECL signal was significantly quenched. Thus, a selective electrochemiluminescence (ECL) aptasensor for BPA was developed. The ECL aptasensor based on Ru(bpy) 3 2+ @MIL-125 has a good ECL response toward detecting BPA. Under optimal conditions, the aptasensor can detect BPA in a wide linear range from 1.0 × 10 −12 to 1.0 × 10 −6 M with an excellent determination limit of 6.1 × 10 −13 M (S/ N = 3). The ECL aptasensor can selectively detect BPA in a mixture containing BPA interference compounds.
研究领域:
分析化学
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）