WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
·
2011
·Vol. 28
·期 3
·857-863
·
IF 1.214
Ye Li,Zhang Chunyan,Li Jiyang,Shi Xunlong,Feng Meiqing
Calcium is a known signalling molecule in eukaryotic cells and plays a central role in the regulation of many cellular processes. In the following study, we report on the effect of external calcium treatments on the biotransformation of ginsenoside Rb1 to ginsenoside Rd by Paecilomyces bainier 229-7. We observed that the intracellular calcium content of P . bainier 229-7 mycelia was increased in response to exposure to high external Ca 2+ concentrations. Both ginsenoside Rd biotransformation and β-glucosidase activity were both found to be dependent on the external calcium concentration. At an optimal Ca 2+ concentration of 45 mM, maximal ginsenoside Rd bioconversion rate of 92.44% was observed and maximal β-glucosidase activity of 0.1778 U was reached in a 72-h biotransformation. The Ca 2+ channel blocker Verapamil blocked the trans-membrane influx of calcium and decreased ginsenoside Rd biotransformatiom. In addition, β-glucosidase activity and ginsenoside Rd content decreased by 36.0 and 29.2% respectively after a 72-h incubation in the presence of 0.05 mM Calmodulin (CaM) antagonist Perphenazine. These results suggest that both Ca 2+ channels and CaM are involved in ginsenoside Rd biotransformation via regulation of β-glucosidase activity. This is the first report regarding the effects of calcium signal transduction on biotransformation and enzyme activity in fungi.
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高影响因子
Journal of Materials Chemistry B
·
2017
·Vol. 5
·期 21
·3970-3981
·
IF 4.543
Mengjun Zhang,Shasha Jing,Jie Zhang,Jiulong Zhang,Xinlong Zang,Mingxi Qiao,Xiuli Zhao,Haiyang Hu,Dawei Chen
Multidrug resistance (MDR) has been a major obstacle to tumor chemotherapy. Pluronic unimers have been reported to be promising copolymers to reverse MDR, and the intracellular delivery of Pluronic unimers is a problem worth thinking. To exert the excellent reversal effect of Pluronic unimers, DOX-loaded G4.0 PAMAM was modified with PluronicL64 via cis-aconitic acid as a pH-sensitive linkage (PCPAMAM/DOX), which could release DOX and Pluronic unimers into cytoplasm. The Pluronic-modified PAMAM (PCPAMAM) exhibited favorable biocompatibility and pH-sensitivity. PCPAMAM/DOX showed a nano-scale size and a sustained in vitro release profile. Compared with a control formulation, PCPAMAM/DOX showed a higher reversal effect on MCF-7/ADR cells and enhanced intracellular drug accumulation. The results of P-gp activity, subcellular distribution of PluronicL64, the ATP level and mitochondrial transmembrane potential all illustrated that free Pluronic unimers could be released by PCPAMAM functioning as reversal agents. In conclusion, PCPAMAM could be a promising vehicle to enhance DOX accumulation by overcoming MDR in MCF-7/ADR cells. This work also provided an effective method to deliver Pluronic unimers into MDR cells.
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CHEMISTRY & BIODIVERSITY
·
2025
·
IF 2.5
Ke Guo,Peng Zhao,Xinxin Yang,Jin Wang,Wei Chang,Simei Liu,Siqi Zhang,Tingting Zhang,Hao Yan,Yafeng Yan,Jie Wang,Xiping Chen,Churong Yu,Guoquan Wang
The present study reports the synthesis of a selenium nanocomplex (Se-TFPs) using purified polysaccharides from Tussilago farfara L (coltsfoot). It evaluates its structural characteristics, physicochemical properties, and inhibitory effects of Michigan Cancer Foundation-7 (MCF-7) breast cancer cells. The influence of processing conditions on nanoparticle size and stability at 25 °C was assessed using particle size and zeta potential measurements. The Se-TFPs were synthesized by optimizing the processing conditions via response surface methodology, yielding nanoparticles with a selenium (Se)-to-polysaccharide mass ratio of 1:13.5, a Se-to-ascorbic acid molar ratio of 1:4.5, a selenite concentration of 10.7 mM, and a reaction time of 4.4 h. The resulting Se-TFPs had an average particle size of 107.2 nm and a zeta potential of -35.1 mV. Structural and physicochemical analyses confirmed successful nanoparticle formation. Compared to TFPs, Se-TFPs exhibited significantly enhanced scavenging activity against 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), hydroxyl radicals, and superoxide anion radicals. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays, flow cytometry, and cell cycle apoptosis analysis revealed that Se-TFPs effectively inhibited MCF-7 cell proliferation at the S phase, with an IC50 value of 119.62 μg/mL.
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Science of Advanced Materials
·
2021
·Vol. 13
·期 7
·1295-1303
·
IF 1.474
Liu Guangheng,Yang Xiangfeng,Niu Qiming,Sun Wenkui
ABSTRACT
A new type of difolate targeting nano-level ultrasound contrast agent ((folate molecule, FOL)2-TUAs) was prepared, so as to investigate its targeted binding effect with human breast cancer mammary carcinoma cells (MCF-7) in vitro. L-2-aminoadipic
acid (L-2-AD) as a branch unit was inserted at the hydroxyl end of distearoyl phosphatidylethanolamine (DISP)-PEG2000-COOH to construct a tree structure. At this time, the free hydroxyl group in the distearoyl phosphatidylethanolamine (DISP)-PEG2000-COOH structure modified the FOL with the
help of N-Hydroxysuccinimide/N,N'-dicyclohexylcarbodiimide (NHS/DCC). Each 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DISP-PEG2000) connected two FOLs to generate difolate targeted nanomaterials. Nano laser particle size (PS)
and Zeta potential analyzer (ZPA) were applied to analyze the physical characteristics of the material such as PS and dispersion, and the enhanced development effect in vitro was detected by the ultrasonic diagnostic instrument. Besides, the targeted binding ability of the contrast
agent based on this material to folate receptor (FR) overexpressing MCF-7 cells was analyzed by flow cytometry (FCM) and fluorescence microscope. In the experiment, hydrogen-1 nuclear magnetic resonance (1H NMR) demonstrated that (FOL)2-TUAs was successfully synthesized.
The surface of this material was round and uniformly distributed without aggregation. According to the relative number of FOL molecules, non-targeted nano-agent (U-TUA), monofolate targeted nano-agent (FOL-TUA), and difolate targeted nano-agent ((FOL)2-TUA) were obtained. The in
vitro imaging showed that different materials exhibited enhanced imaging effects in ultrasonic diagnostic equipment. FCM and fluorescence microscopy both indicated that the difolate TUA could achieve a good binding to MCF-7 cells. Most of the nano-agents were attached to the cell membrane,
surrounded by red fluorophore, namely increasing the FOL content of DISP-PEG2000 chain could enhance the targeted binding ability of tumor cells.
研究领域:
肿瘤学
·
医学影像学
·
药剂学
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CHEMOSPHERE
·
2016
·
IF 3.698
Min-Hua Cui,Dan Cui,Lei Gao,Ai-Jie Wang,Hao-Yi Cheng
A hybrid anaerobic reactor with built-in bioelectrochemical system (BES) has been verified for efficiently treating mixed azo dye wastewater, yet still facing many challenges, such as uncertain reactor construction and insufficient electron donors. In this study, an up-flow hybrid anaerobic reactor with built-in BES was developed for acid orange 7 (AO7) containing wastewater treatment . Cathode and real domestic wastewater both served as electron donor for driving azo dye decolorization. The decolorization efficiency (DE) of AO7 (200 mg/L) in the hybrid reactor was 80.34 ± 2.11% with volume ratio between anaerobic sludge and cathode (VR slu:cat ) of 0.5:1 and hydraulic retention time (HRT) of 6 h, which was 15.79% higher than that in BES without sludge zone. DE was improved to 86.02 ± 1.49% with VR slu:cat increased to 1:1. Further increase in the VR slu:cat to 1.5:1 and 2:1, chemical oxygen demand (COD) removal efficiency was continuously improved to 28.78 ± 1.96 and 32.19 ± 0.62%, but there was no obvious DE elevation (slightly increased to 87.62 ± 2.50 and 90.13 ± 3.10%). BES presented efficient electron utilization, the electron usage ratios (EURs) in which fluctuated between 11.02 and 13.06 mol e − /mol AO7. It was less than half of that in sludge zone of 24.73–32.06 mol e − /mol AO7. The present work optimized the volume ratio between anaerobic sludge and cathode that would be meaningful for the practical application of this hybrid system.
研究领域:
环境工程
·
废水处理
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Phytochemistry Letters
·
2025
·
IF 1.4
Yan-Mei Wu,Jing-Ya Mo,Yong-Yan Mei,Quan Liu,Yuan-Yuan Huang,Bei Zhou,Yi-Hang Li,Yan-Chun Wu,Jing-Quan Yuan
Trichodones D-F ( 1 - 3 ), three previously undescribed polyketide compounds featuring conjugated α - β -unsaturated ketone moieties, were isolated from the endophytic fungus Trichoderma protrudens associated with the semi-mangrove plant Hibiscus tiliaceus Linn. The structural elucidation of these compounds was accomplished through the analysis of 1D and 2D NMR spectra, complemented by HR-ESI-MS data. The absolute configurations were determined through comparative analysis of experimental CD and calculated ECD spectra. Additionally, the cytotoxic activities of compounds 1 - 3 against the human breast cancer cell line MCF-7 and the human hepatocellular carcinoma cell line HepG2 were evaluated using the CCK8 assay. Notably, compound 3 exhibited moderate inhibitory activity against HepG2 and MCF-7 cell lines with IC 50 values of 22.7 μM and 55.1 μM, respectively.
研究领域:
药理学
·
有机化学
·
肿瘤学
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高影响因子
Small Methods
·
2020
·Vol. 5
·期 4
·2001047
·
IF 12.13
Jianqiao Chang,Yu Zhang,Yike Li,Ziwei Han,Fei Tian,Chao Liu,Qiang Feng,Yuguang Wang,Jiashu Sun,Lu Zhang
Detection of mRNA expression in live cells during treatment is a challenging task, despite its importance in tumor biology and potential therapeutic leads. Here a multilayer ratiometric fluorescent nanomachine for live-cell perturbation and imaging of mRNA at single cell resolution is reported. The nanomachines fabricated by microfluidic approaches consist of fluorescent polymeric cores and multiple lipid layers, which can efficiently deliver siRNA and molecular beacons (MBs) to cytosol and then release the cargo in a sequential way. The siRNA molecules released from the outer lipid layers lead to silencing of multidrug resistance 1 (MDR1) gene, and the MBs from the middle lipid layers detect the presence of MDR1 mRNA. The fluorescent ratio of MBs to fluorescent polymeric cores positively correlates with the expression level of MDR1 mRNA in MCF-7/ADR cells during siRNA treatment. The nanomachines provide comparable results with traditional qPCR for quantifying mRNA, showing great potential for modulation and imaging of intratumoral mRNA in vitro and in vivo.
研究领域:
生物技术
·
分子生物学
·
癌症研究
·
纳米技术
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PubMed
高影响因子
Nanoscale
·
2013
·Vol. 5
·期 5
·2107-2113
·
IF 6.233
Leyong Zeng,Wenzhi Ren,Lingchao Xiang,Jianjun Zheng,Bin Chen,Aiguo Wu
Multifunctional Fe3O4–TiO2 nanocomposites with Janus structure for magnetic resonance imaging (MRI) and potential photodynamic therapy (PDT) were synthesized, in which Fe3O4 was used as a MRI contrast agent and TiO2 as an inorganic photosensitizer for PDT. Their morphology, structure, and MRI and PDT performance were characterized, respectively. Moreover, the location of Fe3O4–TiO2 nanocomposites in MCF-7 cells was also investigated by the staining of Prussian blue and alizarin red, respectively. The results showed that the as-prepared Fe3O4–TiO2 nanocomposites had good T2-weighted MRI performance, and the MCF-7 cells incubated with nanocomposites could be killed under the irradiation of UV light. Compared with traditional organic photosensitizers, TiO2 inorganic photosensitizers could have more stable PDT performance due to their nanoscale size and anti-photodegradable stability. Therefore, the as-prepared Fe3O4–TiO2 nanocomposites could have potential applications as a new kind of multifunctional agent for both MRI and PDT.
研究领域:
生物医学工程
·
材料科学
·
医学影像
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ACS Sensors
·
2018
·
IF 5.711
Qian Han,Jianping Liu,Qingtao Meng,Yong-Lei Wang,Huan Feng,Zhiqiang Zhang,Zhi Ping Xu,Run Zhang
An effective bioanalytical method for rapid, sensitive, specific, and in situ sensing of nitric oxide (NO) is the key for further unveiling the biological functions of this gasotransmitter molecule in vitro and in vivo. In this contribution, a new fluorescence probe for sensing and imaging of NO in live systems was developed. The probe, FP-NO, was designed by exploring a novel sensing mechanism, i.e., the rotation of the N–N single bond of a coumarin derivative. FP-NO was prepared by incorporating a recognition unit, thiosemicarbazide moiety into a coumarin fluorophore. The weakly fluorescent FP-NO quickly and selectively reacts with NO to form a highly fluorescent product, FP-P. Such an enhancement of fluorescence emission allows NO detection with high sensitivity. The detection limit was 47.6 nM. The reaction mechanism was validated by HRMS titration analysis and the “OFF-ON” fluorescence response mechanism was rationalized by theoretical computation. FP-NO is biocompatible and live cell membrane permeable. The feasibility of FP-NO as the fluorescence probe for imaging and flow cytometry analysis of exogenous NO in MCF-7 cells and exogenous NO production in inflamed J774A.1 macrophage cells was then evaluated. Visualization of exogenous and endogenous NO production in live zebrafish was then achieved, implying the potential application of FP-NO in the studies of the NO roles in live organisms.
研究领域:
生物化学
·
分子生物学
·
医学影像学
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BIOSENSORS & BIOELECTRONICS
·
2014
·
IF 6.451
Xiaoru Zhang,Mingshuai Liu,Hongxia Liu,Shusheng Zhang
A new photoelectrochemical (PEC) biosensor was developed using low-toxic Ag 2 S QDs as photoelectrochemically active species. Energy levels of Ag 2 S and Ag 2 Se QD were compared to explain their differences in the PEC performance. The preparation condition of Ag 2 S QD was optimized and its structure characterization was measured. Then the developed photoelectric active interface was used to detect glucose and MCF-7 cancer cell and showed the good sensitivity and specificity. Under optimal condition, detection limits of 3.2×10 −5 M for glucose and 98 cells/mL for MCF-7 cell were achieved. Thus, the prepared Ag 2 S QD could serve as an excellent and promising photoelectric active material in the PEC biosensor.
研究领域:
生物传感技术
·
材料科学
·
医学诊断
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COLLOIDS AND SURFACES B-BIOINTERFACES
·
2012
·
IF 3.456
Qian Guo,Ping Luo,Yu Luo,Fang Du,Wei Lu,Shiyuan Liu,Jin Huang,Jiahui Yu
Biodegradable micelles with sheddable poly(ethylene glycol) shells were fabricated based on poly(ethylene glycol)- block -poly(γ-benzyl l -glutamate) (mPEG-SS-PBLG) diblock copolymer and applied as the carrier of 7-ethyl-10-hydroxy-camptothecin (SN-38) in order to enhance its solubility and stability in aqueous media. The diblock polymer was designed to have the hydrophilic PEG moiety and hydrophobic PBLG moiety linked by biodegradable disulfide bond, so in reducing environment the PEG shells can be detached. The polymer was able to form the micelles of nano-scale in aqueous media, suggesting their passive targeting potential to tumor tissue. Water-insoluble antitumor drug, SN-38, was easily encapsulated into mPEG-SS-PBLG nanomicelles by lyophilization method. When setting theoretical drug loading content at 10 wt%, the drug encapsulation efficiency (EE) was assayed as 73.5%. Owing to the disulfide bond in mPEG-SS-PBLG, intense release of SN-38 occurred in the presence of dithiothreitol (DTT) at the concentration of simulating the intracellular condition, however, micelles showed gradual release of SN-38 in the absence of DTT. Also, the mPEG-SS-PBLG micelles effectively protected the active lactone ring of SN-38 from hydrolysis under physiological condition. Compared with free SN-38, SN-38-loaded nanomicelles showed essentially decreased cytotoxicity against L929 cell line in 24 h, bare mPEG-SS-PBLG nanomicelles showed almost non-toxicity.
研究领域:
药学
·
生物医学工程
·
肿瘤学
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PubMed
高影响因子
Analytical Cellular Pathology
·
2021
·
IF 2.916
Xinwei Liu,Yue Zhou
Background. Long noncoding RNAs (lncRNAs) are dysregulated in periodontitis development and involved in osteogenesis. The current study was aimed at investigating the function of lncRNA ANRIL in periodontal ligament cells (PDLCs) and potential molecular mechanisms. Methods. Firstly, the level of ANRIL was tested by qPCR. Then, PDLCs were treated with a mineralizing solution to induce osteogenic differentiation. ALP activity was measured, and protein levels of BMP2, Osterix, and OCN were measured by Western blot. A target of ANRIL was verified using dual-luciferase reporter assay. miR-7 level was measured by qPCR, and the signals of the NF-κB pathway were tested by Western blot. Results. ANRIL expression was downregulated in PDL tissues. Next, ALP activity and protein levels of BMP2, Osterix, and OCN were increased to show that PDLCs were differentiated. ANRIL level was increased in differential PDLCs, in which knockdown inhibited osteogenic differentiation. Then, miR-7 was found as a target of ANRIL. The miR-7 level was upregulated in PDL tissues and reduced in differential PDLCs. Inhibition of miR-7 suppressed ALP activity and BMP2, Osterix, and OCN expression. Moreover, inhibition of miR-7 reversed the effects on the osteogenic differentiation induced by knockdown of ANRIL. Besides, the levels of p-P65 and p-IκBα were elevated by ANRIL downregulation and were rescued by suppressing miR-7. Conclusions. Knockdown of ANRIL inhibited osteogenic differentiation via sponging miR-7 through the NF-κB pathway, suggesting that ANRIL might be a therapeutic target for periodontitis.
研究领域:
分子生物学
·
口腔科学
·
牙周病学
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高影响因子
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