ACTA BIOCHIMICA ET BIOPHYSICA SINICA
·
2018
·Vol. 50
·期 6
·567-578
·
IF 2.224
Zhang Yingying,Cong Linhai,He Jiayan,Wang Yu,Zou Yingying,Yang Zhihong,Hu Yuexin,Zhang Shiwen,He Xiaoguang
Hypopharyngeal carcinoma (HC) is one of the most malignant tumors in the upper aerodigestive tract. Currently, there are no effective treatments for HC. Gold nanoparticles (AuNPs) are a promising tool that can be used for plasmonic photothermal therapy (PPTT), which refers to the use of electromagnetic radiation, most often in near infrared (NIR) region, for the treatment of various medical conditions including cancer. AuNPs have been proved to be a promising tool for NIR spectroscopy-mediated photothermal therapies. In this study, we chemically conjugated AuNPs with a monoclonal antibody (mAb) targeting the epidermal growth factor receptor (EGFR), a cell-surface receptor that is overexpressed in many cancers. We then assessed the effect of NIR photothermal treatment with the EGFRmAb–AuNPs in FaDu HC cells. Our data showed that nanoparticle conjugation with the EGFRmAb improved the specific targeting towards FaDu cells and reduced cytotoxicity towards normal (293 T) cells which do not overexpress the EGFR. A significant amount of our EGFRmAb-conjugated AuNPs could enter the nucleus. Moreover, the expression levels of double strand DNA break repair proteins, including p-ATR, p-CHK1, and p-CHK2 were increased following AuNPs treatment, indicating the presence of DNA damage. These findings suggest that the AuNPs can potentially disrupt genome integrity and induce apoptosis. In addition, EGFRmAb–AuNPs+NIR could induce FaDu cell apoptosis, accompanied by the inhibition of the PI3K/AKT/mTOR pathway and stimulation of DNA damage response. Based on these data, PPTT using the EGFRmAb–AuNPs could be a new promising treatment for HC.
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高影响因子
BIOSENSORS & BIOELECTRONICS
·
2018
·
IF 8.173
Xiaolun Peng,Jinlin Zhu,Wei Wen,Ting Bao,Xiuhua Zhang,Hanping He,Shengfu Wang
In the paper, a triple-amplified biosensor was built for the purpose of ultrasensitive methyltransferase (Dam MTase) activity detection and inhibitor screening based on in-situ synthesized silver nanoclusters (AgNCs) as signal probes to provide amplified current signal coupling with gold nanoparticles/electrochemical reduced graphene oxide (AuNPs/ERGO) hybrids and hybridization chain reactions (HCR) amplification strategy. For biosensor preparation, the AuNPs/ERGO hybrids were firstly generated on the electrode surface by electrochemical co-reduction method, then the ds-DNA structures which comprised the specific recognition sequences (5′-G-A-T-C-3′) of the Dam MTase and the restrictive endonuclease DpnI were formed on the electrode by the hybridization of the assistant DNA with the capture DNA. The presence of Dam MTase methylated partial ds-DNA structures on the electrode which could be digested by DpnI and could not undergo HCR process. The unmethylated ds-DNA structures underwent HCR process to further hybridize with DNA1 and DNA2 to form ds-DNA superstructures as effective template for AgNCs in-situ synthesis. Oxidation peak current of silver was obtained as signal output for Dam MTase detection. Integrated synthesis and detection in one as well as combined metal nanocluster with DNA superstructures, this biosensor showed a good performance for Dam MTase activity detection with a detection limit of 0.0073 U/mL. Compared with reported biosensor, the designed biosensor showed high sensitivity and had potential in clinical diagnosis as well as inhibitor screening.
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ACS Applied Materials & Interfaces
·
2015
·
IF 6.723
Guanru Chang,Yunlong Wang,Baoyou Gong,Yazhong Xiao,Yan Chen,Shaohua Wang,Shikuo Li,Fangzhi Huang,Yuhua Shen,Anjian Xie
Integration of multimodal treatment strategies combined with localized therapy to enhance antitumor efficacy and reduce side effects is still a challenge. Herein, a novel composite hydrogel containing rGO, amaranth extract (AE) and gold nanoparticles (AuNPs) was prepared by using AE as both reductant and cross-linking agent. The chlorophyll derivatives in AE were also employed as a photodynamic therapy drug. Meanwhile, AuNPs and rGO both have obvious photothermal effects and can accelerate the generation of cytotoxic singlet oxygen (1O2). The temperature increase of rGO/AE/AuNPs precursor is up to 6.3 °C under 808 nm laser irradiation at a power density of 200 mW·cm–2. The hydrogel shell on in situ tumor cells was easily formed and regulated by near-infrared irradiation within 10 min, which could both retain a high concentration of drugs on the lesion site and prevent them from migrating to normal tissue, thus reducing the side effects. Compared with rGO/AE and AE, rGO/AE/AuNPs showed a remarkably improved and synergistic antitumor effect. The hydrogel possesses good biocompatibility and high hydrophilicity and could be used for loading chemotherapeutics, which provides a new approach for located and multiple antitumor therapies.
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ELECTROCHEMISTRY COMMUNICATIONS
·
2017
·
IF 4.396
Jie Zhang,Wensheng Tan,Yongxin Tao,Linhong Deng,Yong Qin,Yong Kong
SiO 2 nanospheres were synthesized by the sol–gel method using tetraethoxysilane (TEOS) as the precursor, which was then modified with 3-aminopropyltriethoxysilane (APTES) for the grafting of NH 2 to the SiO 2 surface. Au nanoparticles (AuNPs) were anchored to the NH 2 grafted SiO 2 , and then l -cysteine (L-Cys) templates were introduced to the SiO 2 /AuNPs through forming Au S bond. Another layer of SiO 2 was then coated on the L-Cys doped SiO 2 /AuNPs as the coating layer, and then the templates were removed by calcination to form a sandwich-structured molecularly imprinted SiO 2 /AuNPs/SiO 2 . Finally, the molecularly imprinted chiral interface was applied for electrochemical enantioselective recognition of Cys isomers, and discernible differences in the current signals could be observed on the differential pulse voltammograms ( I D-cys / I L-cys , 2.36).
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BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Su Liu,Yu Wang,Wei Xu,Xueqi Leng,Hongzhi Wang,Yuna Guo,Jiadong Huang
In this paper, a novel sandwich-type electrochemical aptasensor has been fabricated and applied for sensitive and selective detection of antibiotic oxytetracycline (OTC). This sensor was based on graphene-three dimensional nanostructure gold nanocomposite (GR-3D Au) and aptamer-AuNPs-horseradish peroxidase (aptamer-AuNPs-HRP) nanoprobes as signal amplification . Firstly, GR-3D Au film was modified on glassy carbon electrode only by one-step electrochemical coreduction with graphite oxide (GO) and HAuCl 4 at cathodic potentials, which enhanced the electron transfer and loading capacity of biomolecules . Then the aptamer and HRP modified Au nanoparticles provide high affinity and ultrasensitive electrochemical probe with excellent specificity for OTC. Under the optimized conditions, the peak current was linearly proportional to the concentration of OTC in the range of 5×10 −10 −2×10 −3 g L −1 , with a detection limit of 4.98×10 −10 g L −1 . Additionally, this aptasensor had the advantages in high sensitivity, superb specificity and showed good recovery in synthetic samples. Hence, the developed sandwich-type electrochemical aptasensor might provide a useful and practical tool for OTC determination and related food safety analysis and clinical diagnosis.
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高影响因子
ELECTROANALYSIS
·
2017
·Vol. 30
·期 2
·320-327
·
IF 2.851
Shasha Feng,Xiaoping Wei,Li Zhong,Jianping Li
A novel molecularly imprinted polymer (MIP) photoelectrochemical sensor was fabricated for the highly sensitive and selective detection of triclosan. The MIP photoelectrochemical sensor was fabricated using graphite-like carbon nitride (g-C 3 N 4 ) and gold nanoparticles (AuNPs) as photoelectric materials. The MIP/g-C 3 N 4 -AuNPs sensor used photocurrent as the detection signal and was triggered by ultraviolet light (UV-Light 365 nm). g-C 3 N 4 -AuNPs was immobilized on indium tin oxide electrodes to produce the photoelectrochemically responsive electrode of the MIP/g-C 3 N 4 -AuNPs sensor. A MIP layer of poly- o -phenylenediamine was electropolymerized on the g-C 3 N 4 -AuNPs-modified electrode to act as the recognition element of the MIP/g-C 3 N 4 -AuNPs sensor and to enable the selective adsorption of triclosan to the sensor through specific binding. Under optimal experimental conditions, the designed MIP/g-C 3 N 4 -AuNPs sensor presented high sensitivity for triclosan with a linear range of 2×10 −12 to 8×10 −10 M and a limit of detection of 6.01×10 −13 M. Moreover, the MIP/g-C 3 N 4 -AuNPs sensor showed excellent selectivity. The sensor had been successfully applied in the analysis of toothpaste samples.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Jing Liu,Yan Ping Chen,Penglin He,Ziyu Ding,Yun Guo,Songhe Cui,Chao Ma,Zhiping Xie,Sun Xia,Yin Zhang,Ye Liu,Yuan Liu
Hexanal is considered as an important volatile compound indicator for the assessment of freshness and maturity of foods. Therefore, sensitive and stable monitoring of hexanal is highly desired. Herein, an efficient receptor immobilization strategy based on ZIF-8@ Single-walled carbon nanotube (SWCNT) and nanosomes-AuNPs/Prussian blue (PB) was proposed for the development of olfactory biosensors. ZIF-8@SWCNT as dual support materials provided a high density of active sites for nanosomes loading. Moreover, the co-electrodeposition of nanosomes-AuNPs and PB on the sensor interface effectively amplified the electrochemical signal and maintained the activity of the receptor. The combination of ZIF-8@SWCNT with AuNPs/PB imparts excellent sensing performance of the biosensor with a wide detection range of 10 −16 –10 −9 M, a low detection limit of 10 −16 M for hexanal, and a long storage stability of 15 days. These results indicate that our biosensor can be a powerful tool for versatile applications in food and other related industries.
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Analytical Methods
·
2024
·
IF 2.7
Zhiren Zhou,Yuting Tian,Lina Zou,Yanan Liu,Xueqing Zhang,Xiaojing Huang,Huanyu Ren,Zheng Li,Huiru Niu,Hao Liao,Xiaojing Zhang,Hongzhi Pan,Shengzhong Rong,Hongkun Ma
Nuclear matrix protein 22 (NMP22) is one of the most important tumor markers of bladder cancer and is significantly elevated in the urine of bladder cancer patients. Therefore, in this work, a highly sensitive ratiometric electrochemical immunosensor was constructed to detect NMP22 based on ZIF-8@MWCNTs@Chit@Fc@AuNPs composites. ZIF-8 had a large surface area and good adsorption ability. Multi-Walled Carbon Nanotubes (MWCNTs) can optimize the electrical conductivity of ZIF-8, so that the electrode surface of ferrocene (Fc) obtains a stable and strong electrochemical signal. In addition, AuPt-MB provided another strong detection signal methylene blue (MB) while immobilizing the secondary antibody (Ab2) through Au–N and Pt–N bonds. A ratiometric electrochemical sensor was formed based on ZIF-8@MWCNTs@Chit@Fc@AuNPs and AuPt-MB, which showed a great linear connection between IMB/IFc and the logarithmic concentration of NMP22 with a detection limit of 3.33 fg mL−1 (S/N = 3) under optimized specifications in the concentration interval of 0.01 pg mL−1 to 1000 ng mL−1. In addition, the ratiometric immunosensor showed good selectivity and stability.
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高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 6
·1-10
·
IF 5.3
Zhong Yujun,Li Zhiyang,Zhang Anlin,Peng Yuqing,Zhou Hao,Wang Bin,Xie Lianwu,Guo Yaping
A novel electrochemical sensor, MIP/Cu-MOF/rGO/AuNPs/GCE, was developed by depositing gold nanoparticles, coating Cu-MOF/GO on the surface of glassy carbon electrode (GCE) before electroreducing graphene oxide (GO) to rGO and covering molecularly imprinted membrane by electropolymerization for highly sensitive detection of electroneutral organophosphorus pesticide residues in agricultural product. Cyclic voltammetry, differential pulse voltametry, scanning electron microscopy, energy-dispersive spectroscopy, and atomic force microscopy were used to characterize the imprinted sensor. Several key factors such as chitosan concentration, suspension volume, pH of polymerization solution, and polymerization scanning rate during preparation of the imprinted sensor were optimized in detail. When electroneutral phosmet was used as a template, the linear range of MIP/Cu-MOF/rGO/AuNPs/GCE for detecting phosmet was 1.00 × 10 −14 –5.00 × 10 −7 mol/L with the limit of detection of 7.20 × 10 −15 mol/L at working potentials of − 0.2 to 0.6 V. The selectivity, reproducibility, and repeatability of MIP/Cu-MOF/rGO/AuNPs/GCE were all acceptable. The recoveries of this method for determining phosmet in real samples ranged from 94.2 to 106.5%. The MIP/Cu-MOF/rGO/AuNPs/GCE sensor could be applied to detect electroneutral pesticide residues in organisms and agricultural products. Graphical Abstract
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IEEE SENSORS JOURNAL
·
2024
·
IF 4.3
Tengfei Yang,Pengxiang Chang,Ailing Zhang,Fei Liu,Honggang Pan,Junfeng Wang,Guangxiao Cao,Mingxuan Zheng,Zhao Zhang
A PDA/AuNPs/PDANPs sandwich structure modified optical fiber surface plasmon resonance (SPR) biosensor for specific detecting of rabbit IgG were proposed in this paper. On the proposed sensor, polydopamine (PDA), gold nanoparticles (AuNPs) and polydopamine nanoparticles (PDANPs) were sequentially deposited to form a multilayer hybrid structure. The refractive index (RI) sensitivity of the sensor has been significantly enhanced through the strong coupling effects of SPR and localized surface plasmon resonance. The sensor achieves an RI sensitivity of3009.58 nm/RIU, representing a 3-fold increase compared to the original SPR sensor. In addition, due to the high surface activity and large specific surface area of PDANPs, the biosensitivity of the sensor has been enhanced. The sensor demonstrated specific detection of Rabbit IgG with a biosensitivity of 0.9157 nm/(μg/mL) and limit of detection (LOD) of 22 ng/mL. These findings indicate that the prepared sensor can specifically detect Rabbit IgG with high precision and a low LOD. Therefore, this PDA/AuNPs/PDANPs sandwich structure modified biosensor has great potential in clinical detection and other bio-detection fields.
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TALANTA
·
2019
·
IF 4.916
Xiangyu Ma,Haoran Xu,Kun Qian,Martha Kandawa-Schulz,Weimin Miao,Yihong Wang
MicroRNAs (miRNAs) are important biomarkers in early diagnosis of disease. In this work, we developed a simple and effective electrochemical biosensor based on Au nanoparticles (AuNPs)/Carbon nitride nanosheet (CNNS) nanocomposite for the miRNAs detection. Duplex-specific nuclease (DSN) and hairpin structure probe were utilized to improve the sensitivity and selectivity respectively. In the presence of miRNA-21, the signal molecule could be released from the surface of the electrode and decrease the current peak in square wave voltammetry (SWV) test. Under the optimal conditions, the reported biosensor showed that the detection range of miRNA-21 is from 10 fM to 1 nM and detection limit is as low as 2.9 fM. Furthermore, the detection of miRNA-21 added in serum samples indicates the developed biosensor with good selectivity, stability and reproducibility which verify its potential to be used in the early diagnosis of diseases.
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ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Zongmei Zheng,Mingyang Wang,Jinlong Yuan,Junchong Liu,Haipeng Yu,Zhonghuang Yang,Wanjian Liu,Aihua Liu
It is crucial to timely and accurately identify the causative virus for early treatment and urgent prevention. Viral antigen detection can identify those people who are most likely at risk of spreading the disease, but most based on antibodies with limited stability and sensitivity. Peptides offer several advantages over antibodies, such as low cost, smaller size and good stability. The development of electrochemical immunoassay using specific peptide probes have the merits of good sensitivity and selectivity as well as good stability. Herein we report an amperometric immunosensor using peptides as capture probe and recognition probe. The molecular docking suggests that the two peptides of Pi (sequence: NFWISPKLAFALGGGKKKSC) and FK11 (sequence: WFLNDSELISML), bioscreened from phage display, bind to N-terminal domain of SARS-CoV-2 spike protein (SP). The peptide Pi is assembled on MoS 2 @AuNPs modified electrode to capture SARS-CoV-2 SP, which is recognized by peptide FK11-displayed phage to form Pi/SARS-CoV-2 SP/FK11-phage sandwich. Then anti-M13 phage conjugated horseradish peroxidase (HRP) (anti M13-HRP) was introduced to recognize the phage capsid protein pVIII to form M13 phage/anti M13-HRP to enrich thousands of HRP, which can further electrochemically catalyze H 2 O 2 reduction at highly conductive MoS 2 @AuNPs at − 0.35 V. Then amperometric immunosensor was constructed with linear range of 0.1−5000 pg/mL SARS-CoV-2 SP and detection limit of 0.074 pg/mL. The sensor also has good selectivity, batch reproducibility and stability, capable of detecting down to 10 transducing units/mL SARS-CoV-2 pseudoviruses. This work represents the first example of dual-peptide probes based sandwich-type electrochemical immunosensor integrated with dual signal amplification, which may provide a cost-effective assay platform in detecting real SARS-CoV-2 viruses for early diagnosis. The flexible and modular strategy can be extended to develop other type biosensors for a wide range of applications.
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危险品化学品经营许可证（带存储）