Anti-Cancer Agents in Medicinal Chemistry
·
2013
·Vol. 13
·期 8
·1280-1290
·
IF 2.61
Wen Hua Yang,Zhen Zhang,Yang Hu Sima,Jian Zhang,Jian Wen Wang
Glaucocalyxin (Gla) A-C are major ent-kauranoid diterpenoids isolated from Rabdosia japonica var. glaucocalyx, a plant used in Chinese traditional medicine as an antitumor and anti-inflammatory agent. The present investigation was carried out to observe whether cellular reduced glutathione (GSH) plays important roles in Gla -induced cytotoxicity. Among major ent-kauranoid diterpenoids isolated, Gla A and B dose-dependently decreased the growth of HL-60 cells with an IC50 of approximately 6.15 and 5.86 µM at 24 h, respectively. Both Gla A and B could induce apoptosis, G2/M-phase cycle arrest, DNA damage and the accumulation of reactive oxygen species (ROS) in HL-60 cells. Moreover, Gla A, B caused rapid decrease of the intracellular GSH content, while inhibition of cellular GSH synthesis by buthionine sulfoximine (BSO) augmented the induced cytotoxicity and apoptosis in HL-60 cells. On the other hand, the administration of GSH or GSH precursor N-acetyl-cysteine (NAC) could rescue Gla A, B-depleted cellular GSH, and abrogate the induced cytotoxicity, G2/M-phase cycle arrest, DNA damage and ROS accumulation in HL-60 cells. Furthermore, Gla A, B decreased the activity of the GSH-related enzymes including glutathione reductase (GR) and glutathione peroxidase (GPX). These data suggest that the intracellular GSH redox system plays important roles in regulating the Gla A, B-induced cytotoxicity on HL-60 cells.
研究领域:
药理学
·
生物化学
·
肿瘤学
·
细胞生物学
·
天然产物化学
✓ 已验证
DOI
高影响因子
Materials
·
2025
·Vol. 18
·期 23
·
IF 3.2
Wenjie Zhou,Tao Du,Changbai Long
High-efficiency, lead-free dielectrics are sought for pulsed-power capacitors, yet pristine Bi0.5Na0.5TiO3(BNT) suffers from large remanence, high coercivity, and limited breakdown strength. Here, we report (1 − x)Bi0.5Na0.5Ti0.97Nb0.03O3-xSr0.85Ba0.15Ta0.5+0.02xAl0.5−0.02xO3(BNTNb–SBTA, x = 0–0.15) ceramics synthesized via solid-state reaction, achieving enhanced relaxor ferroelectric behavior through multi-cation substitution at A- and B-sites. X-ray diffraction confirms a pure perovskite solid solution, while scanning electron microscopy reveals grain refinement, suppressing oxygen vacancies and boosting the breakdown strength. Raman and dielectric analyses evidence strengthened relaxor behavior, accompanied by loop slimming and a systematic rise in breakdown strength. The composition x = 0.10 achieves the best trade-off, deliveringWrec= 3.357 J cm−3andη= 90.5% atEb= 240 kV cm−1. Robust operational stability is demonstrated with small variations ofWrec/ηover 0.1–200 Hz, 25–175 °C, and 106cycles. Pulsed tests show fast discharge (∼26 ns) withWd= 0.826 J cm−3at ∼90% efficiency under moderate fields. These results indicate that synergistic A/B-site disorder (Sr/Ba on A-site; Ta/Al with Nb on B-site), combined with microstructural densification, effectively minimizesPrwhile elevatingEb, enabling high-efficiency energy storage under practical operating conditions.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2015
·
IF 4.152
Jianwei Du,Ce Tian,Yajie Liu,Jun Ling,Youxiang Wang
Polypeptide has been extensively researched in gene/drug delivery system due to the good biocompatibility. Herein, we synthesized total-polypeptide copolymers, i.e. Azo(azobenzene)-capped polysarcosine- b -polylysine (ASL) with narrow molecular weight distribution by α-amino acid N-carboxyanhydride (NCA) polymerization. Although the molecular weight of PLL segment was only about 6 kDa, ASL could condense DNA effectively and form about 150 nm spherical nanoparticles at N/P ratio of 15. The surface charge was significantly reduced due to the shielding effect of polysarcosine (PSAR) shell. ASL/DNA PeptoPlexes showed good colloidal stability under physiological salt condition and complexation competition stability in the presence of counter polyanion, which might improve the circulation time in vivo . The tip design of azobenzene provided a facile way for ligand modification via host–guest interaction, which could be flexibly optimized by changing its functional tags responding to a request. Our data showed that the introduction of CD-R8 could promote the internalization of gene into cytoplasm and even nucleus owing to the membrane penetrating effect of R8. Cell culture experiments indicated as a total-polypeptide system, ASL showed good cellular viability and comparable gene transfection level as PLL with molecular weight of 50 kDa. Overall, PSAR served as an ideal alternative of PEG and this total-polypeptide system showed us a good direction for gene carrier design.
研究领域:
材料科学
·
基因治疗
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Soft Matter
·
2018
·Vol. 14
·期 36
·7420-7428
·
IF 3.709
Dinglei Zhao,Quan Tang,Qiang Zhou,Kang Peng,Haiyang Yang,Xingyuan Zhang
As one of the most promising biomaterials, injectable self-healing hydrogels have found broad applications in a number of fields such as local drug delivery. However, controlled release of drugs in hydrogels is still difficult to realize up to now. Here, we report a novel photo-degradable injectable self-healing hydrogel based on the hydrophobic interaction of a biocompatible four-arms star polymer, poly(ethylene glycol)-b-poly(γ-o-nitrobenzyl-L-glutamate). The hydrophobic interaction between poly(γ-o-nitrobenzyl-L-glutamate) not only connects poly(ethylene glycol)-b-poly(γ-o-nitrobenzyl-L-glutamate) together with a crosslink but also provides a hydrophobic domain to encapsulate hydrophobic pharmaceuticals such as doxorubicin (DOX). Due to the dynamic character of the hydrophobic interaction, the hydrogel exhibits excellent injectable and self-healing ability. In particular, the photolabile o-nitribenzyl ester group is cleaved under UV irradiation. As a result, the hydrophobic domain transforms into the hydrophilic one and the embedded DOX is released effectively. An increasing release ratio of DOX dramatically enhances the apoptosis ratio of HeLa cells. We expect these attractive properties may be beneficial to practical applications of the hydrogel as an effective local drug delivery means in a truly physiological environment.
研究领域:
生物医学工程
·
药学
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2021
·Vol. 248
·期 2
·553-565
·
IF 2.998
Wang Liling,Qin Yuchuan,Wang Yanbin,Liu Bentong,Zhou Yifeng
In this research, the inhibition mechanism of a pairs of homoisoflavonoid analogues (6-aldehydo-isoophiopogonanone A (AIO-A) and 6-aldehydo-isoophiopogonanone B (AIO-B)) on tyrosinase (Tyr) was investigated by multi-spectroscopic techniques combined with molecular docking. The results demonstrated that AIO-A suppressed the activity of Tyr in a reversible competitive-inhibition with a half inhibitory concentration (IC50) of (12.67 ± 2.52) × 10 –5 molˑL −1 , while AIO-B was a reversible mixed-inhibition with IC50 of (4.81 ± 0.74) × 10 –5 molˑL −1 . AIO-A/AIO-B bound to Tyr driven by the intermolecular interactions, including van der Waals interaction, hydrogen bonding and hydrophobic interaction, thereby resulted in a secondary structure change of Tyr and its intrinsic fluorescence quenching. The molecular docking revealed that an aldehyde oxygen atom in AIO-A and methoxyphenyl in AIO-B formed coordination with two Cu ions in the active center of Tyr protein. It can be deduced that the coordination with two Cu ions may prevent the entrance of substrate and then inhibit the catalytic activity of Tyr. The research might provide new perspectives on the inhibition mechanism of AIO-A and AIO-B on Tyr and functional research for the prevention and treatment of pigmented skin diseases and anti-browning of homoisoflavonoids as a food supplement.
研究领域:
生物化学
·
药理学
·
植物化学
✓ 已验证
DOI
高影响因子
CERAMICS INTERNATIONAL
·
2025
·
IF 5.6
Han Xu,Gangsong Huang,Wenting Zhou,Xuan Miao,Hongjun Ji,Mingyu Li,Yufeng Li
In the realm of electronic packaging advancements, the utilization of metal to ceramic joints has garnered significant attention. This manuscript details the development of a specific glass solder with excellent wettability and a thermal expansion coefficient of 8.1 × 10 −6 °C −1 , comprising 35Bi 2 O 3 -30B 2 O 3 -15ZnO-20SiO 2 (mol.%). This design achieves reliable connection of the Al 2 O 3 ceramics and Kovar alloy in an atmospheric environment. The interface reaction between the glass solder, Al 2 O 3 ceramics, and oxidized Kovar alloy resulted in the formation of ZnAl 2 O 4 particles and Bi spheres at the joints. The enhancement in joint shear strength was primarily attributed to the nanoscale, dispersed ZnAl 2 O 4 particles. However, the presence of microscale Bi spheres, with a lower elastic modulus than the glass solder, initiated cracks during shear deformation, thereby compromising the integrity of the joint. Optimization of brazing temperatures enabled control over the ZnAl 2 O 4 and Bi sphere phases within the joints, yielding a peak shear strength of 51.7 ± 8.9 MPa at 650 °C. This study highlights the achievement of high-performance metal-ceramic bonding through tailored interface reactions and optimized joint microstructures.
研究领域:
材料科学
·
电子封装
·
连接技术
·
陶瓷-金属连接
·
玻璃焊料
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL PHYSICS
·
2020
·
IF 1.771
Lizhi Zhao,Yaxiang Zhao,Rishun Li,Dihao Wu,Xingyu Xie,Hui Ye,Yuzhong Zhang,Qingping Xin
Metal-organic frameworks (MOFs) of copper-tetrakis-(4-carboxyphenyl)-porphyrin (CuTCPP) were prepared by a solvothermal route. The two-dimensional (2D) ultrathin nanosheets with larger specific surface area show higher adsorption capacity for rhodamine B (RhB), compared with the bulk MOF of CuTCPP. The binding mechanism of the 2D nanosheets with RhB was investigated based on spectroscopic methods . The results reveal that the fluorescence of RhB is efficiently quenched by 2D CuTCPP nanosheets , and the quenching mechanism is totally the static quenching by forming a ground state RhB-CuTCPP nanosheets complex with an apparent association constant of 3.953 × 10 3 M −1 . The interaction between RhB and CuTCPP nanosheets leads to the H-aggregate of RhB in the complex. Higher-order aggregate may also be involved in the complexation. Thermodynamic studies demonstrate that RhB-CuTCPP nanosheets interaction is a spontaneous process and driven by electrostatic and hydrophobic forces . The 2D CuTCPP nanosheets can be regenerated from the complex and used repeatedly.
研究领域:
材料科学
·
光谱学
·
环境科学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Haiyang Wang,Lin Cai,Yuwei Wang,Chang Liu,Guozhen Fang,Shuo Wang
A molecularly imprinted electrochemical sensor (MIES) modified with Cu-MOF and Ti 3 C 2 Tx was successfully prepared for the detection of hygromycin B in food. According to the pH sensitive property of reversible borate ester bonds, a molecularly imprinted polymers (MIP) were constructed on the modified gold electrode (GE) surface by electropolymerization to realize the controllable elution and adsorption of template molecules. Cu-MOF with large specific surface area and Ti 3 C 2 Tx with excellent electrical conductivity cooperate to improve the overall analytical performance of MIES. The morphology and electrochemical performance of the sensor were evaluated. Under the optimal experimental conditions, the method showed superior selectivity to hygromycin B with a linear range of 5 × 10 −9 –5 × 10 −6 M and a detection limit of 1.92 × 10 −9 M (S/N = 3). The detection results of this method are in good agreement with HPLC-MS/MS, which proves that the method has reliable practicability and accuracy.
研究领域:
食品科学
·
分析化学
·
生物传感技术
✓ 已验证
DOI
高影响因子
Journal of Zhejiang University-SCIENCE A
·
2015
·Vol. 16
·期 8
·669-679
·
IF 0.882
Shan Jie,Wang Li,Yu Hao-jie,Tai Yu-lei,Akram Muhammad
Porous polymers are very suitable materials for the adsorption of organic pollutants due to their abundant pores and organic frameworks in aqueous solution. However, their recovery from treated pollutant is difficult, and thus, their application is limited. A facile strategy to synthesize reusable magnetic porous microspheres (MPMS) of Fe 3 O 4 /poly(methylmethacrylate (MMA)- co -divinylbenzene (DVB)) is described in this paper. The magnetic microspheres were synthesized by suspension copolymerization. MMA was used as a monomer, DVB was used as a crosslinker, and the magnetic fluid was added to the organic phase. The morphology of MPMS was observed by scanning electron microscope (SEM) and other properties were tested by superconducting quantum interference device, Fourier transform infrared (FT-IR) spectroscopy, thermogravimetric analysis (TGA), X-ray powder diffraction (XRD), and nitrogen sorption-desorption techniques. The synthesized MPMS possessed a high specific surface area using toluene as a porogen. It was further found that the ratio of DVB to MMA, the ratio of porogen to monomer, and the type of porogen all affected the specific surface area and the morphology of the microspheres. Furthermore, the microspheres were applied to remove Rhodamine B from its aqueous solution. The results showed that the microspheres possessed good adsorption capacity for Rhodamine B. This result was due to the porous structure, polar groups, and superparamagnetic characteristic of the synthesized microspheres.
研究领域:
材料科学
·
环境化学
·
高分子化学
·
吸附分离
·
功能材料
✓ 已验证
DOI
高影响因子
HIGH PERFORMANCE POLYMERS
·
2017
·
IF 1.179
Ying Sun,Xiaojing Ding,Xueqin Zhang,Qizan Huang,Baoping Lin,Hong Yang,Lingxiang Guo
A series of conjugated copolymers based on indeno[1,2-b]fluorene as donor unit with different acceptor units have been synthesized to explore the influences of molecular backbone planarization and acceptor electronegativity on charge transport and photovoltaic properties. Polymer incorporating 2,3-diphenylquinoxaline acceptor moiety shows poor light-harvesting capacity and inferior photovoltaic efficiency of 0.5% due to twisted geometry. By introducing the stronger acceptor thiadiazolo[3,4-c]pyridine in polymer, intramolecular charge transfer is enhanced, giving rise to improved absorption property and photovoltaic efficiency of 1.39%. However, the polymer backbone is still twisted. When thiophene-flanked diketopyrrolopyrrole (DPP) is incorporated as electron acceptor, the polymer exhibits a more planar molecular geometry, yielding a broader and red-shifted absorption spectrum as well as a significantly improved hole mobility of 1.46E-2 cm2 V−1 s−1. However, the photovoltaic device efficiency is only enhanced to be 1.69%. The low-lying lowest unoccupied molecular orbital of −3.95 eV as a result of the strong electron deficiency of the DPP unit may lead to the inefficient charge dissociation and increase the charge recombination, which may give rise to the limit photovoltaic performance.
研究领域:
材料科学
·
有机电子学
·
光伏技术
✓ 已验证
DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2019
·
IF 2.983
Baofang Zhang,Zilin Chen
A simple and valid method for rapid screening of cathepsin B inhibitors from traditional Chinese medicines (TCMs) was established by the combination of immobilized enzyme microreactor (IMER) and capillary electrophoresis. Cathepsin B was immobilized on the inner surface of the capillary by glutaraldehyde method. The separation of substrate and product could be finished by baseline within 3 min. The activity of the immobilized cathepsin B remained approximately 90% after 50 runs. The quantification and statistical analysis of the product peak area was used to evaluate the catalytic activity of cathepsin B. The value of Michaelis-Menten constant of cathepsin B was 0.85 mM. The half-maximal inhibitory concentration (IC 50 ) of L -trans-Epoxysuccinyl-leucylamido(4-guanidino)butane (E-64) was measured as 36.08 nM, which indicated that the cathepsin B reactor was successfully developed and was feasible for inhibitorscreening. The raised method was then applied to discover the inhibitory potential of 17 standard compounds from traditional Chinese medicines. Five natural products, including kaempferol, rutaecarpine, evodiamine, theophylline, lycobetaine showed potential inhibition for cathepsin B. Additionally, molecular docking study was investigated for supporting the interaction between enzyme and inhibitors.
研究领域:
药理学
·
生物化学
·
药物化学
·
酶学
✓ 已验证
DOI
PubMed
高影响因子
Environmental Technology & Innovation
·
2025
·
IF 7.1
Shuzhen Yang,Bingjing Tian,Yanfang Huang,Bingbing Liu,Shengpeng Su,Wenjuan Wang,Guihong Han
The persistent dye rhodamine B (RhB) in textile wastewater poses severe ecological threats, demanding efficient and sustainable treatment technologies. This study synthesized a novel trimetallic Cu-Fe-Co catalyst via a nitrogen-atmosphere co-reduction method. The catalyst’s unique advantage lies in the synergistic effects of Cu, Fe, and Co, which significantly enhance catalytic performance. The redox-active phases of Cu, combined with the Fenton-like reactivity of Fe, are further optimized by Co doping to improve reactive oxygen species (ROS) generation and electron transfer efficiency. Characterization revealed a dendritic structure dominated by Cu, Cu₂O, and FeO phases, with Co-induced lattice expansion (Δ d / d = +0.25 %) enhancing surface reactivity. Under optimized conditions (pH 3, 1.5 g/L catalyst, 20 mg/L RhB), the ternary Cu-Fe-Co catalyst achieved 99.8 % RhB degradation within 40 min, with high mineralization efficiency (46.5 % TOC removal, 82.1 % COD removal). Mechanistic studies demonstrated that Co incorporation promotes the conversion of superoxide radicals (·O₂⁻) into long-lived singlet oxygen (¹O₂), synergistically enhancing oxidative effects from ·OH and ¹O₂, while in situ H₂O₂ generation drives radical chain reactions. Compared to binary catalysts, the ternary system exhibits superior degradation kinetics for RhB and maintains 95.8 % degradation efficiency even after five cycles. This study provides new insights into trimetallic catalyst design, highlighting its practical potential for wastewater treatment.
研究领域:
环境科学
·
催化
·
材料科学
·
化学工程
·
水处理技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）