Virulence
·
2025
·
IF 5.4
Hao-Jie Shen,Jun-Feng Wang,Zheng-Jie Xue,Jia-Qi Shi,Bing Chen,Dong-Min Chen,Ni-Pi Chen,Yu-Dong Li,Chao-Dong Qian
Antimicrobial resistance is one of the greatest threats to global health. A thorough comprehension of the underlying processes through which bacteria evolve the ability to withstand diverse stressors is vital for the development of efficacious strategies to address this challenge. This study aimed to elucidate the mechanisms underlying the adaptation of an acrB-deficient strain of Escherichia coli ATCC 35218 (35218m) to sanguinarine (SAN), the main component of phytobiotic derived from Macleaya cordata. Analysis of the in vitro-selected SAN-resistant clones of strain 35218m led to the identification of a functionally uncharacterized TetR regulator, EefR. The regulator represses its own expression and that of four neighboring genes forming the eefRABCD operon, encoding components of a tripartite RND-efflux pump (EefABC) and a putative MFS-type exporter (EefD). Overexpression of these pumps reduces the susceptibility of acrB-deficient E. coli to SAN and structurally diverse antibiotics. The eefR-eefABC-eefD cluster, widely distributed across Enterobacteriaceae genomes, is also found on plasmids in several E. coli isolates, indicating a significant risk for the rapid spread of this multidrug resistance mechanism.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF THERMAL SPRAY TECHNOLOGY
·
2013
·Vol. 22
·期 6
·1053-1062
·
IF 1.481
Liu Yuxin,Huang Jing,Ding Siyue,Liu Yi,Yuan Jianhui,Li Hua
Nanostructured titania has been extensively investigated as photocatalytic material and is capable of killing bacteria attached on its surface. The persistent challenge yet is how to effectively promote adhesion of bacteria on its surface for consequent extermination. The study presented here deals with liquid flame-sprayed nanostructured titania-hydroxyapatite (HA) coatings. Addition of HA alleviated phase transformation of titania from anatase to rutile during the coating deposition, reducing rutile to anatase ratio from 9.58 to 1.99%, and precluded effectively aggregation of the nano titania particles in the as-sprayed coatings. Adherence of Escherichia coli bacteria on the coatings showed significant dependence on content of HA, and the increased HA content resulted in enhanced attachment of the bacteria. Examination of the photocatalytic activity of the coatings through decomposition of methylene blue dye in water revealed that addition of HA did not markedly deteriorate the photocatalytic performances of the coatings. The coatings consisting of 10 wt.% HA showed the best photocatalytic activity, which is comparable to that exhibited by immobilized Degussa P25 coatings. The unambiguous evidence provided in this study suggests that the coatings made from combination of biocompatible HA and photocatalytic nano titania have great potential for antibacterium applications.
✓ 已验证
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2026
·
IF 7.3
Xiaoyi Wang,Chao Xie,Jiaxin Liao,Xiaoli Wang,Dirk Mayer,Yuting Zhang,Nandi Zhou
Microelectrode arrays (MEAs) hold inherent potential for the parallel analysis of foodborne pathogens; however, their miniaturized scale introduces critical challenges for multitarget detection, including cross-talk between adjacent units during functionalization and a severely limited sensing area that restricts probe immobilization and ultimate sensitivity. To overcome these limitations, a MEA-based aptasensor is constructed by integrating site-selective electrodeposition with a directed molecular allocation strategy for highly sensitive and specific simultaneous detection of Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). A graphene oxide-polypyrrole nanocomposite and an AuNPs/chitosan-gold composite were precisely deposited on different microelectrode units, enabling the spatially resolved immobilization of aptamers against S. aureus and E. coli, respectively. Fluorescence and Raman spectroscopy confirmed the selective allocation of the respective aptamers to their targeted electrode units, effectively preventing cross-talk. Using methylene blue as an electroactive probe, the binding of target pathogens to their respective aptamers was quantified by square wave voltammetry. With optimized conditions, the limit of detection for S. aureus and E. coli was as low as 25.4 CFU mL–1 and 8.8 CFU mL–1. Real-sample assays confirmed that the MEA-based aptasensor is a precise and viable tool for high-speed quantification of S. aureus and E. coli, exhibiting considerable potential for applications in environmental and food safety monitoring practices.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
VETERINARY RESEARCH
·
2026
·Vol. 57
·期 1
·175
·
IF 3.8
Jiang Luyao,Yang Fan,Wu Jiayan,Li Zhengliang,Zhu Xiemin,Pan Hao,Zhang Shaotong,Pan Haochun,Wang Hang,Yao Jingting,Yan Ping,Gao Qingqing,Huan Changchao,Gao Song,Jiao Xinan,Liu Xiufan
Host defense peptides (HDPs) possess both direct bacteriocidal and immunomodulatory activities. In this study, chicken cathelicidin-1 (cCATH-1) and chicken cathelicidin-3 (cCATH-3) were selected based on their markedly different minimum inhibitory concentrations (MICs) against avian pathogenic Escherichia coli (APEC) strains, with MICs of cCATH-1 being 4- to 16-fold lower than those of cCATH-3. However, following intraperitoneal challenge with APEC strain E058 (4 × 10⁶ CFU/mouse), 80% of cCATH-3 treated mice survived while 20% of cCATH-1 treated mice survived. Flow cytometry (FC) revealed significantly lower peripheral blood Ly6C high monocytes but higher peritoneal Ly6C high macrophages in cCATH-3-treated mice at 4 and 8 h post-treatment. Peritoneal CCL3 secretion was significantly upregulated in cCATH-3 treated mice at 8 h post-treatment. Upon knockout of macrophages, the protection of cCATH-3 in mice against APEC E058 challenge was markedly abolished. In vitro, peritoneal macrophages treated with cCATH-3 showed stronger bacteriocidal activity than those treated with cCATH-1. Mouse CCL-3 (mCCL3)-specific antibody neutralize the effect of mCCL3 on macrophages chemotaxis in vitro. mCCL3-specific antibody administration resulted in 0% survival in cCATH-3-treated mice versus 60% survival in cCATH-3-treated mice administered with mock antibody, while cCATH-1-treated mice showed 0% survival administered with either mCCL3-specific antibody or mock antibody after APEC E058 strain challenge. In summary, cCATH-3 provides robust protection against APEC infection compared with cCATH-1 in mice, partially through CCL3-mediated macrophage chemotaxis and enhanced bacteriocidal activity in activated macrophages, revealing a novel mechanism of avian cathelicidins and supporting HDPs as alternatives to antibiotics for controlling avian colibacillosis.
✓ 已验证
DOI
高影响因子
Frontiers in Veterinary Science
·
2026
·
IF 3.1
Yu Ren,Lingkang Liu,Wenya Zheng,Ben Liu,Zerui Zhao,Xunhao Zhu,Lisen He,Songlin Ding,Wei Hu,Lucheng Zheng,Qingcan Fan,Hailong Hong
IntroductionCalf diarrhea caused by Escherichia coli poses severe economic burdens to the cattle industry, and antibiotic overuse has triggered drug resistance and residue risks. This study screened 10 plant extracts for antibacterial activity against a clinically isolated calf diarrhea-causing E. coli strain in vitro, and investigated the protective effects of epigallocatechin gallate (EGCG)—the most effective extract—on E. coli-infected mice.MethodsAntimicrobial susceptibility was assessed by disk diffusion, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) assays. In vivo, mice were intraperitoneally challenged with E. coli and treated with 50 mg/kg or 100 mg/kg EGCG. Bacterial loads in liver and small intestine were quantified, intestinal pathology was evaluated by HE staining, and expression of tight junction proteins (ZO-1, Occludin, Claudin-1) was analyzed by immunohistochemistry and Western blotting.ResultsEGCG exhibited the largest inhibition zone (19.7 ± 0.3 mm) against the pathogen, with an MIC of 3.125 mg/mL and MBC of 50 mg/mL. In vivo, both 50 mg/kg and 100 mg/kg EGCG significantly reduced bacterial loads in the liver and small intestine of infected mice, alleviated small intestinal pathological damage, and the 100 mg/kg EGCG group showed markedly upregulated expression of ZO-1, Occludin, and Claudin-1 in the small intestine (p < 0.05).DiscussionOur findings confirm that EGCG exerts potent in vitro antibacterial effects against calf diarrhea-causing E. coli and protects against E. coli-induced small intestinal injury in mice, highlighting its potential as a natural antibiotic alternative for controlling colibacillary diarrhea in calves.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·
IF 6.7
Bingbing Liu,Lin Jiang,Lvyang Lin,Wulong Yang,Mian Li,Jianping Lin,Yiqi Jiang,Zehua Bao,Mianbin Wu
Xylitol is industrially synthesized by d-xylose, which is more expensive than glucose. However, de novo xylitol biosynthesis from glucose remains challenging in Escherichia coli. To address these challenges, this study first developed a biosynthetic pathway from glucose via an arabitol intermediate with arabitol phosphate dehydrogenase (APDH). Chassis optimization through knocking out key genes (rpiA/B, pgi, tktA, tktB, xylB) enhanced flux and increased arabitol yield to 1.61 g/L. Subsequently, the arabitol-to-xylitol route was established using arabitol dehydrogenase (ArDH2) and xylitol dehydrogenase (XDH1) enzymes, showing a 52.1% conversion rate in vitro. The heterologous AXA module (APDH, ArDH2, XDH1) was multicopy integrated into the optimized chassis W3P-5ΔB. The strain W3P4A produced 5.04 g/L arabitol and 1.89 g/L xylitol in shake-flasks. Fed-batch fermentation with a plasmid-carrying strain achieved 5.11 g/L xylitol and 18.5% (g/g) yield from glucose. This work establishes a new platform for xylitol bioproduction from glucose and provides a foundational framework for industrial-scale implementation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Functional Materials Letters
·
2024
·
IF 1.2
Wenge Liang,Tao Gao,Jia Yi
With the continuous exposure of major food safety issues in recent years, food safety has become the focus of people’s attention. As a widely distributed bacterium, excessive levels of Escherichia coli in food may cause gastrointestinal discomfort symptoms, as well as adverse consequences such as food poisoning, acute gastroenteritis, food allergies, and gastric ulcers. Therefore, to ensure food safety and achieve rapid detection of E. coli , this study uses CdS quantum dots (QDs) nanomarkers to detect E. coli in water. Moreover, electrochemical square wave voltammetry is used to determine the relationship between Cadmium (Cd) ion concentration and E. coli concentration in the complex after enzyme-linked immunosorbent reaction. The experiment showed that the average particle size of CdS QDs nanoparticles was 5 nm and they had good biocompatibility. The current magnitude of electrochemical square wave voltammetry was positively correlated with the concentration of Cd ions and E. coli . When the concentration of E. coli was 50-1.0 × 1 0 5 cfu/mL, its concentration was linearly positively correlated with the magnitude of the current, with a linear correlation coefficient of 0.9873. Compared to the flat plate counting method, its relative error did not exceed 10%, and in addition, the detection time was only 2 h. The above results indicate that CdS QDs nanomarkers can achieve rapid detection of E. coli , effectively improve the detection sensitivity of E. coli , and weaken non-specific adsorption.
✓ 已验证
DOI
高影响因子
GEOMICROBIOLOGY JOURNAL
·
2015
·
IF 1.44
Xing Liu,Wenqiang Zhao,Qiaoyun Huang,Peng Cai
Attachment of Escherichia coli to soil particles influences bacteria transport to water sources. Nontoxigenic E. coli are commonly used as indicators for predicting the fate of pathogens and their contamination potential in natural environments. However, few studies have been conducted to compare the attachment of indicator bacteria with pathogenic bacteria in soil environments. Therefore, the objectives of this study were to compare pathogenic E. coli O157:H7 and nonpathogenic E. coli WH09 attachment to different soils and to assess the effects of soil physicochemical properties on E. coli attachment on soil particles. E. coli O157:H7 cell surfaces in tested soil suspensions were much less negatively charged and more hydrophobic than E. coli WH09, which resulted in greater amounts of E. coli O157:H7 attachment to soil particles. Pearson correlation analysis between partition coefficient (Kd) and soil properties showed the attachment of E. coli O157:H7 to soil particles was negatively correlated with organic matter (OM) of soils (p < 0.01); however, E. coli WH09 attachment was positively correlated with soil solution electrical conductivity (EC) (p < 0.01). Partial correlation and stepwise multiple regression analysis suggested OM and clay content (CC) were the dominant factors (p < 0.01) impacting E. coli O157:H7 attachment. However, EC was the sole significant factor (p < 0.01) determining the attachment of E. coli WH09 to soil particles.
✓ 已验证
DOI
高影响因子
Frontiers in Cellular and Infection Microbiology
·
2026
·
IF 5.5
Yujuan Li,Yi Yang,Ting Zhou,Jing Zhou,Jie Hu,Shuangshuang Liang,Zhenhua Xie,Qing Cheng,Yachao Jiang,Chao Jiang,Qing Liu
Amid the growing antibiotic resistance crisis, colistin remains a last-line therapeutic option for multidrug-resistant (MDR) Gram-negative bacterial infections. However, the emergence and rapid dissemination of plasmid-mediated colistin resistance gene (mcr) have markedly reduced its clinical effectiveness. Utilizing colistin adjuvants to restore its antibacterial potency is a promising strategy to combat this threat. In this study, we aimed to investigate the synergistic effects of α-terpineol in combination with colistin against colistin-resistant MDR Escherichia coli (E. coli) and to elucidate the underlying mechanisms of this synergy. In vitro synergistic activity was evaluated using checkerboard microdilution assays, time-kill curve analyses, and resistance development studies. Mechanistic insights were obtained through transcriptomic analysis and fluorescence-probe based assays. In vivo efficacy was validated using Galleria mellonella and mouse acute peritonitis infection models. The results showed that the combination of α-terpineol and colistin exhibited synergistic bactericidal activity and suppressed the development of colistin resistance. α-Terpineol increased inner- and outer-membrane fluidity in E. coli, thereby enhancing colistin uptake. Furthermore, it inhibited superoxide dismutase (SOD) activity, preventing the conversion of O2·– to H2O2 and causing superoxide accumulation. Elevated O2·– levels damaged iron–sulfur clusters and impaired respiratory function. Together with the proton motive force (PMF) dissipation, these effects severely impaired ATP synthesis, ultimately sensitizing MDR E. coli to colistin. The combination also showed significant therapeutic efficacy in animal models. Our findings identify α-terpineol as a promising colistin adjuvant that restores antibacterial activity by disrupting membrane integrity and impairing antioxidant defenses, offering a viable strategy for treating MDR Gram-negative bacterial infections.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Electrochemical Science
·
2023
·Vol. 12
·期 4
·3443-3458
·
IF 1.5
Shichao Xu,Yiyi Zhang,Kai Dong,Junnan Wen,Chunming Zheng,Shihuai Zhao
In this article, we present a simple and sensitive electrochemical DNA biosensor based on graphene oxide (GO)/chitosan (CS) hybrid nanocomposites modified glassy carbon electrode (GCE) for detection of Escherichia coli O157:H7 (E.coli O157:H7). The morphology and composition of GO and hybrid nanocomposites were characterized by transmission electron microscope (TEM), X-ray powder diffraction (XRD), field emission scanning electron microscopy (FESEM), and Fourier transform infrared spectrum (FTIR). Cyclic voltammetry investigations indicated that the GO/CS/GCE showed excellent electron transfer ability and good linear relation. Under the optimal hybridization conditions, electrochemical impedance spectroscopy (EIS) responses of ssDNA/GO/CS/GCE biosensor were in linear with the target DNA in the concentration range from 1×10 -14 to 1×10 -8 M with the detection limit as 3.584×10 -15 M (3σ). Moreover, differential pulse voltammetry (DPV) studies revealed good specificity and excellent ability of ssDNA/GO/CS/GCE biosensor to distinguish complementary, 1-base mismatched DNA, 2-base mismatched DNA and multi-base mismatched DNA. The developed strategy in this research revealed that the GO/CS modified electrode possess excellent performance for detecting of Escherichia coli O157:H7 DNA.
研究领域:
生物传感器
·
分子诊断
·
纳米医学
·
食品安全检测
·
微生物学
✓ 已验证
DOI
高影响因子
Journal of Hazardous Materials Advances
·
2026
·
IF 9.1
Wenqi Li,Ying Cao,Quanzhen Yang,He Liu,Stefanos Giannkis,Jin Jiang
UVA-driven iron-mediated sulfite advanced oxidation processes have demonstrated high efficiency in degrading organic pollutants, yet their potential for microbial inactivation remains underexplored. This study systematically investigates the disinfection performance and mechanism of a UVA/Fe(III)/sulfite system using Escherichia coli ( E. coli ) as a model bacterium. The ternary system exhibited synergistic bactericidal activity, achieving complete inactivation of 10 6 CFU/mL E. coli within 45 min at pH 5.6, significantly outperforming individual or binary controls. Radical quenching and electron paramagnetic resonance (EPR) analyses identified sulfate radicals ( SO 4 • − ) , hydroxyl radicals ( • OH ) , superoxide ( O 2 • − ) , and peroxymonosulfate radicals ( SO 5 • − ) as the primary reactive species, generated via a sustained photochemical Fe(II)/Fe(III) redox cycle. Mechanistic investigations revealed a multi-target oxidative cascade: initial disruption of cell envelope integrity and lipid peroxidation facilitated intracellular radical influx, leading to rapid adenosine triphosphate (ATP) depletion, inactivation of antioxidant enzymes (superoxide dismutase (SOD) and catalase (CAT)), and oxidative deoxyribonucleic acid (DNA) lesions. Disinfection efficiency increased with decreasing pH, and was promoted by elevated dissolved oxygen. While chloride and nitrate exhibited moderate interference, phosphate and humic acid severely inhibited inactivation through Fe(III) precipitation and radical scavenging/light screening, respectively. These findings elucidate the inactivation pathways of the UVA/Fe(III)/sulfite system, establishing a theoretical foundation for its application as a cost-effective, solar-driven water disinfection strategy.
✓ 已验证
产品关联
DOI
高影响因子
Science Advances
·
2023
·Vol. 9
·期 7
·
IF 13.6
Yu-Jia Wang,Lian Li,Jie Yu,Hong-Yu Hu,Zi-Xu Liu,Wen-Jie Jiang,Wei Xu,Xue-Ping Guo,Feng-Shan Wang,Ju-Zheng Sheng
The introduction of unnatural chemical moieties into glycosaminoglycans (GAGs) has enormous potential to facilitate studies of the mechanism and application of these critical, widespread molecules. Unnatural N-acetylhexosamine analogs were metabolically incorporated into the capsule polysaccharides of Escherichia coli and Bacillus subtilis via bacterial metabolism. Targeted metabolic labeled hyaluronan and the precursors of heparin and chondroitin sulfate were obtained. The azido-labeled polysaccharides (purified or in capsules) were reacted with dyes, via bioorthogonal chemistry, to enable detection and imaging. Site-specific introduction of fluorophores directly onto cell surfaces affords another choice for observing and quantifying bacteria in vivo and in vitro. Furthermore, azido-polysaccharides retain similar biological properties to their natural analogs, and reliable and predictable introduction of functionalities, such as fluorophores, onto azido-N-hexosamines in the disaccharide repeat units provides chemical tools for imaging and metabolic analysis of GAGs in vivo and in vitro.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）