Journal of Pharmaceutical Analysis
·
2023
·
IF 8.8
Si-Yu Gui,Xin-Chen Wang,Zhi-Hao Huang,Mei-Mei Li,Jia-Hao Wang,Si-Yin Gui,Gan-Hua Zhang,Yao Lu,Li-Ming Tao,Hai-Sheng Qian,Zheng-Xuan Jiang
Diabetic retinopathy (DR) is a prevalent microvascular complication of diabetes and the leading cause of blindness and severe visual impairment in adults. The high levels of glucose trigger multiple intracellular oxidative stress pathways, such as POLDIP2, resulting in excessive reactive oxygen species (ROS) production and increased expression of VCAM-1, HIF-1α, and VEGF, causing microvascular dysfunction. Dihydromyricetin (DMY) is a natural flavonoid small molecule antioxidant. However, it exhibits poor solubility in physiological environments, has a short half-life in vivo, and has low oral bioavailability. In this study, we present, for the first time, the synthesis of ultra-small Fe-DMY nano-coordinated polymer particles (Fe-DMY NCPs), formed by combining DMY with low-toxicity iron ions. In vitro and in vivo experiments confirm that Fe-DMY NCPs alleviate oxidative stress-induced damage to vascular endothelial cells by high glucose, scavenge excess ROS, and improve pathological features of DR, such as retinal vascular leakage and neovascularization. Mechanistic validation indicates that Fe-DMY NCPs can inhibit the activation of the Poldip2-Nox4-H 2 O 2 signaling pathway and downregulate vital vascular function indicators such as VCAM-1, HIF-1α, and VEGF. These findings suggest that Fe-DMY NCPs could serve as a safe and effective antioxidant and microangio-protective agent, with the potential as a novel multimeric drug for DR therapy.
研究领域:
药学
·
纳米技术
·
眼科学
·
分子生物学
·
内分泌学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2019
·
IF 3.579
Wei Xu,Nan Zhang,Zhentao Zhang,Pu Jing
Anthocyanins exist likely as complexes with flavones/metal ions in some plants. However, few studies indicate the complexation effects on bioactivities, i.e. antioxidant activities. The aim of this study was to evaluate effects of complexes of cyanidin-3-diglucoside-5-glucoside (CY3D5G) with rutin/Mg(II) on intrinsic antioxidant enzymes and mRNA expressions. All complexes exhibited cellular antioxidant activity in RAW264.7 cells, whereas half maximal effective concentration (EC 50 ) values of CY3D5G complexes were significantly lower than its alone. Combination indexes of CY3D5G-rutin, CY3D5G-Mg(II), and CY3D5G-rutin-Mg(II) complexes were < 1 ( p < .05), indicating of synergistic effects. Additionally, CY3D5G-Mg(II) rather than others significantly enhanced total antioxidant capacity, superoxide dismutase activity, and glutathione peroxidase activity while reducing malonic dialdehyde contents in H 2 O 2 -induced RAW264.7 cell model ( p < 0.05), which were further confirmed with the up-regulated mRNA expression of nuclear factor E2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1), and the down-regulated p21 ( p < 0.05). Our results demonstrated that CY3D5G and its complexes might enhance cellular antioxidant activities via the Nrf2/HO-1 signaling pathway. Additionally, a synergistic antioxidant effect of CY3D5G in the present of rutin/ Mg(II) as regular food components was evident, suggesting anthocyanin might interact with dietary components such as Mg(II) or rutin in food matrix or biological systems and exhibit a stronger antioxidant activity than it alone. The Mg (II) that exhibited no antioxidant activities alone shows potential as a co-adjuvant for the anthocyanin antioxidant activity.
研究领域:
药理学
·
生物化学
·
细胞生物学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2022
·
IF 4.598
Qiong Liu,Kaiyue Hu,Yuqi She,Yufang Hu
Cancer cells require higher ROS levels to maintain their growth state of rapid division and spread·H 2 O 2 , as a kind of usual ROS molecule, is monitored by designing a new in-situ growth G4-nanowire-driven electrochemical biosensor, which is further used for probing H 2 O 2 released by rat astroglioma cell. In this process, the electrocatalytic performance to H 2 O 2 of G4-nanowire (containing G-quadruplex/hemin nanostructures) is employed as signal output, which is constructed by terminal deoxynucleotidyl transferase (TdT)-mediated polymerization in the dNTPs pool of dTTP and dGTP (4: 6). Then, the method is applied for H 2 O 2 monitoring in the range of 0.0003 ∼ 6 mM and the detection limit is estimated to be 0.075 μM (S/N = 3). More importantly, the content of H 2 O 2 generated in rat astroglioma cell is also calculated roughly, displaying an excellent feasibility for the related physiological and pathological studies. Later, TdT activity and its inhibitors are also probed orderly, and a wide linear range is presented with a detection limit of 0.81 mU/mL. It is hoped that the designed platform will provide a reference for exploring new ways to continuous dynamic surveillance of diseases in diagnosis and treatment stage.
研究领域:
分析化学
·
生物化学
·
癌症研究
·
分子生物学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2023
·
IF 5.8
Yang Wang,Wenhui Zha,Ji Wang,Shuo Dong,Chao Liu,Yuhao Jiang,Xinsong Li
Chemodynamic therapy (CDT) has emerged as a powerful tumor treatment option by inducing the imbalance of redox homeostasis in cancer cells. Nevertheless, the therapeutic outcomes were greatly limited because of insufficient endogenous H 2 O 2 and upregulated cellular antioxidant defense in the tumor microenvironment (TME). Herein, a liposome-incorporated in-situ alginate hydrogel locoregional treatment strategy was developed, which involves using hemin-loaded artesunate dimer liposomes (HAD-LP) as redox-triggered self-amplified C-center free radical nanogenerator to enhance CDT. First, HAD-LP based on artesunate dimer glycerophosphocholine (ART-GPC) was prepared by a thin film method. Their spherical structure was manifested by dynamic light scattering (DLS) and transmission electron microscope (TEM). The generation of C-center free radicals from HAD-LP was carefully evaluated by using methylene blue (MB) degradation method. The results suggested that the hemin was reduced to heme under the action of glutathione (GSH), which could catalyze the breakage of endoperoxide of ART-GPC derived dihydroartemisinin (DHA) to generate toxic C-centered free radicals in a H 2 O 2 and pH-independent manner. Moreover, the change of intracellular GSH and free radical level was monitored through ultraviolet spectroscopy and confocal laser scanning microscope (CLSM). It was revealed that the hemin reduction induced GSH depletion and elevated free radical level, disrupting cellular redox homeostasis. After co-incubation with MDA-MB-231 or 4 T1 cells, HAD-LP was found to be highly cytotoxic. In order to prolong retention and improve antitumor efficacy, HAD-LP was mixed with alginate and injected intratumorally into 4 T1 tumor bearing mice. The injected HAD-LP and alginate mixture formed in-situ hydrogel and achieved best antitumor effect with the growth inhibition rate of 72.6%. Together, the hemin-loaded artesunate dimer liposome-incorporated alginate hydrogel possessed effective antitumor activity through redox-triggered C-center free radical generation induced apoptosis in a H 2 O 2 and pH-independent manner, which might be a promising candidate in the application of chemodynamic anti-tumor therapy.
研究领域:
药学
·
纳米技术
·
癌症治疗
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2019
·
IF 3.206
Can Chen,Shiyu Zhang,Chengwu Zhang,Lin Li,Jixin Zhu,Jinhua Liu
Detection of H 2 O 2 is significant for the applications in environmental protection, pharmaceutical industries , food production, and clinical control. Current colorimetric kit of H 2 O 2 based on enzyme or nanomaterials always needs TMB or other peroxidases substrate as coloration species. Herein, we report a simple hydrothermal method to synthesize of TMB dot as the nanosubstrate construction colorimetric kit for highly selective and sensitive detection of H 2 O 2 based on Fenton reaction . Compared with TMB as the substrate, the synthesized TMB dots (TMBDs) show higher reaction activity in the presence of the same low-level Fe 2+ and H 2 O 2 , resulting in highly sensitive sensing H 2 O 2 down to 0.8 nM. Additionally, the obtained TMBDs can well disperse in solution, while generally TMB as substrate needs to be dissolved in organic solvents such as DMF and DMSO. Furthermore, the developed colorimetric kit is applied in detection of Mao-A, due to the reaction between epinephrine and Mao-A producing H 2 O 2 . More importantly, the proposed method has been successfully applied to the analysis of Mao-A in spiked human serum Hence, the TMBDs as the nanosubstrate provide an efficient, fast and sensitive approach for colorimetric kit of H 2 O 2 and Mao-A, promoting more extensive application in early diagnosis of disease and drug discovery.
研究领域:
分析化学
·
生物化学
·
环境科学
·
药学
·
临床化学
✓ 已验证
产品关联
DOI
高影响因子
Advanced Science
·
2023
·Vol. 10
·期 9
·2206851
·
IF 15.1
Peidong Du,Yanzhe Shen,Baoli Zhang,Shan Li,Minzheng Gao,Ting Wang,Xiaokang Ding,Bingran Yu,Zhen-Gang Wang,Fu-Jian Xu
Photodynamic therapy (PDT) is a light triggered therapy by producing reactive oxygen species (ROS), but traditional PDT may suffer from the real-time illumination that reduces the compliance of treatment and cause phototoxicity. A supramolecular photoactive G-quartet based material is reported, which is self-assembled from guanosine (G) and 4-formylphenylboronic acid/1,8-diaminooctane, with incorporation of riboflavin as a photocatalyst to the G4 nanowire, for post-irradiation photodynamic antibacterial therapy. The G4-materials, which exhibit hydrogel-like properties, provide a scaffold for loading riboflavin, and the reductant guanosine for the riboflavin for phototriggered production of the therapeutic H 2 O 2 . The photocatalytic activity shows great tolerance against room temperature storage and heating/cooling treatments. The riboflavin-loaded G4 hydrogels, after photo-irradiation, are capable of killing gram-positive bacteria (e.g., Staphylococcus aureus ), gram-negative bacteria (e.g., Escherichia coli ), and multidrug resistant bacteria (methicillin-resistant Staphylococcus aureus ) with sterilization ratio over 99.999%. The post-irradiated hydrogels also exhibit great antibacterial activity in the infected wound of the rats, revealing the potential of this novel concept in the light therapy.
研究领域:
材料科学
·
生物医学工程
·
药学
·
伤口愈合
·
抗菌治疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Wei Yang,Chenyuan Weng,Xiaoyun Li,Hongliang He,Jianwen Fei,Wei Xu,Xiaoqiang Yan,Wanying Zhu,Hongsong Zhang,Xuemin Zhou
At present, artificial mimic enzyme has become a powerful substitute for natural enzyme. In particular, the biosensors based on nano-enzymes have been the focus of current research. Herein, a novel magnetic ternary nanocomposite hemin-Fe 3 O 4 @polypyrrole (h-Fe 3 O 4 @ppy) was synthesized by chemical oxidation polymerization. The results showed that the h-Fe 3 O 4 @ppy had stronger peroxidase activity than Fe 3 O 4 nanoparticles (Fe 3 O 4 NPs) and h-ppy NPs, and was indicated by Michaelis-Menten constant (K m ) and maximum initial velocity (V max ). Therefore, we used h-Fe 3 O 4 @ppy catalyzing H 2 O 2 to oxidize the chromogenic substrate TMB, and built Hydrogen peroxide (H 2 O 2 ) and Glutathione (GSH) colorimetric biosensors with the help of signal intrinsic exponential amplification and easy operation. The controllable catalysis was realized by h-Fe 3 O 4 @ppy which was prone to separation. The method of removing h-Fe 3 O 4 @ppy by magnetic separation effectively eliminated the effect of residual catalysis when terminating the reaction. The sensor was then applied to the determination of GSH in biological samples from acute coronary syndrome patients. The linear ranges of H 2 O 2 and GSH were 0.2–100 μM and 0.5–80 μM, respectively. The detection limits of H 2 O 2 and GSH are 0.18 μM and 0.15 μM, respectively. This research not only proposes a new type of nanoenzyme, but also shows the multiple advantages of magnetic nano-enzymes for the construction of biosensors.
研究领域:
分析化学
·
生物化学
·
纳米技术
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2024
·
IF 5.3
Tao Ge,Bingyi Zhou,Jingyao Xu,Yulu Wei
Ionic liquids (ILs) were selected and applied to investigate the best coal desulfurization through orthogonal tests. FTIR, XPS, SEM and contact angle tests were used to characterize the effect on the physical and chemical structures in coal for revealing the coal desulfurization mechanism by ILs-H 2 O 2 system. The orthogonal test results showed that reaction temperature and reaction time were the main influencing factors for the desulfurization rates of coal in the ILs-H 2 O 2 system, and the ILs synergy could effectively improve the desulfurization rates of H 2 O 2 . The maximum coal desulfurization rates of [BMIM][HSO 4 ], [BMIM][OTF], and [BMIM][BF 4 ] ionic liquids were 30.7%, 30%, and 33.2%, respectively. The results indicated that the alkyl groups in coal were oxidized and the contents of oxygen-containing functional groups such as hydroxyl and carbonyl increased. Coal surface underwent swelling, the internal pores increased and new pores and cracks were generated. The wettability of coal improved substantially. In addition, the physical structures changes in coal facilitated the contact between the extractant and the sulfur-containing structures. The contents of thiols and sulfides in coal significantly decreased and were oxidized to sulfoxides and sulfones, with some further oxidized to soluble sulfites or sulfates. Thus achieving removal from coal.
研究领域:
环境科学
·
化学工程
·
材料科学
✓ 已验证
DOI
高影响因子
Nanoscale
·
2021
·Vol. 13
·期 29
·12553-12564
·
IF 7.79
Hai-Yan Wang,Zheng-Chen Su,Xi-Wen He,Wen-You Li,Yu-Kui Zhang
Chemodynamic therapy (CDT), the ability to transform H2O2 into a highly toxic hydroxyl radical (˙OH) through a Fenton or Fenton like reaction to kill cancer cells, enables selective tumor therapy. However, the effect is seriously limited by the insufficiency of endogenous H2O2 in cancer cells. Additionally, the specific recognition of epitope imprinting plays an important role in targeting cancer cell markers. In this work, we prepared H2O2 self-supplying degradable epitope molecularly imprinted polymers (MIP) for effective CDT, employing fluorescent calcium peroxide (FCaO2) as an imaging probe and a source of H2O2, the exposed peptide in the CD47 extracellular region as the template, copper acrylate as one of the functional monomers and N,N‘-bisacrylylcystamine (BAC) as a cross-linker. MIP with recognition sites can specifically target CD47-positive cancer cells to achieve fluorescence imaging. Under the reduction of glutathione (GSH), the MIP were degraded and the exposed FCaO2 reacted with water to continuously produce H2O2 in the slightly acidic environment in cancer cells. The self-supplied H2O2 produced ˙OH through a Fenton like catalytic reaction mediated by copper ions in the MIP framework, inducing cancer cell apoptosis. Therefore, the MIP nano-platform, which was capable of specific recognition of the cancer cell marker, H2O2 self-supply and controlled treatment, was successfully used for targeted CDT.
研究领域:
癌症治疗
·
材料科学
·
生物医学工程
·
化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2025
·
IF 4.7
Yun Deng,Shukui Shi,Di Zhao,Mohan Zu,Lu Pang,Xin Li,Zhihao Du,Xiaoli Jin,Zhengdao Li,Fengyun Su,Haiquan Xie
Photocatalytic H 2 O 2 production suffers from severe charge recombination and sacrificial agent dependency. Hydrangea-like Co-doped CdIn 2 S 4 superstructures (Co/CIS (HD)) synthesized via hydrothermal method exhibit hierarchical porous architecture that enhances light harvesting and reactant diffusion. Atomic Co doping introduces mixed-valence Co 2+ /Co 3+ species, reducing the work function from 6.287 to 5.896 eV and extending carrier lifetime. The dual-valent system establishes a self-regenerative redox cycle where Co 3+ mediates photogenerated electron transfer for O 2 activation while Co 2+ scavenges holes to suppress recombination and photocorrosion. Without sacrificial agents, the optimized 6.5-Co/CIS (HD) achieves an exceptional H 2 O 2 production rate of 1952 μmol g −1 h −1 under visible light, 6.33 times higher than pristine CdIn 2 S 4 . The catalyst maintains structural stability over five cycles due to dynamic Co 2+ /Co 3+ regeneration. This work provides a strategic design for efficient photocatalytic H 2 O 2 synthesis via synergistic morphological and electronic engineering.
研究领域:
材料科学
·
光催化
·
环境化学
·
能量转换
·
纳米材料
✓ 已验证
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2023
·
IF 7.7
Yu Wang,Yuxin He,Yujing Chi,Peiji Yin,Lishan Wei,Wenwen Liu,Xinyao Wang,Han Zhang,Haiyan Song
Photocatalytic H 2 O 2 production is a green and sustainable technology, which still exposes the issues of excessive dependence on organic electron donors and pure O 2 , and lacking effective use. It is necessary to develop more efficient and more economical photocatalysis system for H 2 O 2 production. Here, we construct a S-scheme p-n heterojunction from the p-type MnS and the n-type protonated g-C 3 N 4 (PCN) semiconductors for photocatalytic H 2 O 2 production and achieve the in situ use of H 2 O 2 for oxidative degradation of oxytetracycline (OTC). The PCN/MnS composite synthesized by a one-step method well maintains a nanosphere structure of α-MnS and load the PCN on the sphere surface to form a heterojunction with strong interaction. PCN/MnS exhibits the improved photogenerated charge separation and electron transfer efficiency. An optimal photocatalyst can produce 669.6 μM of H 2 O 2 for 6 h without using the electron donors and the pure O 2 gas. The mechanism proposed the contribution of a S-scheme p-n heterojunction forms between PCN and MnS. The electrons in conduction band (CB) of MnS are responsible for the major single-electron reduction of •O 2 - radicals and the auxiliary two-electron reduction of O 2 for H 2 O 2 production, while the holes in valence band (VB) of PCN further promote the separation of photocarriers. The photogenerated H 2 O 2 can be effectively used as an in-situ oxidant to achieve 82.2% degradation of OTC in water within 80 min. The •O 2 - radicals which originate from the in situ decomposition of H 2 O 2 over PCN/MnS mainly contribute to the OTC oxidation .
研究领域:
材料科学
·
环境科学
·
光化学
·
催化
✓ 已验证
产品关联
DOI
高影响因子
Advanced Healthcare Materials
·
2024
·
IF 10
Wei Li,Daomei Chen,Chungang Min,Xiaoqian Ma,Xikun Yang,Jiaqiang Wang
The intervention of endogenous H 2 O 2 via nanozymes provides a potential antitumor-specific therapy; however, the role of the nanozyme structure in relation to the selective decomposition of H 2 O 2 to hydroxyl radicals (•OH) is yet to be fully understood, which limits the development of this therapeutic approaches. Herein, an iron single-atom nanozyme (Fe─N 2 Cl 2 ─C SAzyme) is reported, which is prepared through precise Fe─Cl coordination based on the construction of a characteristic Fe-containing molecule. Fe─N 2 Cl 2 ─C exhibits efficient catalytic H 2 O 2 decomposition (2.19 × 10 6 m m −1 s −1 ), which is the highest among reported SAzymes. More importantly, it is found that H 2 O 2 selectively decomposed into •OH on the Fe─N 2 Cl 2 ─C surface, which is attributable to the d orbitals of the Fe active center matching the O-2 p electrons of the adsorbed hydroxide (*OH) intermediate. Fe─N 2 Cl 2 ─C is strongly cytotoxic toward a variety of cancer-cell lines in vitro but not to normal cells. Furthermore, Fe─N 2 Cl 2 ─C shows an outstanding specific therapeutic effect in vivo; it efficiently destroys solid malignant tumors without injuring normal tissue. Altogether, these findings highlight the selective catalytic decomposition of H 2 O 2 to •OH, which is achieved by engineering the active center on the atomic level, thereby providing an avenue for the development of specific nanomedicines with efficient antitumor activities.
研究领域:
材料科学
·
纳米技术
·
生物医学工程
·
癌症研究
·
化学生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）