MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Zhe Wang,Jiamin Wang,Yu He,Ming Yu,Junping Wang
We present the first electrochemiluminescence (ECL) biosensor integrating N-Acetyl-L-cysteine (NALC)-functionalized upconversion nanoparticles (UCNPs) with reduced graphene oxide (COOH-rGO) for the covalently immobilization of tyrosinase (TYR), and ultrasensitive detection of catechol, a highly toxic and recalcitrant aquatic pollutant. The NALC layer establishes a dense array of enzyme-anchoring sites on the COOH-rGO electrode while preserving TYR activity and extending its operational lifetime. The ECL signal originates from TYR-mediated redox cycling between catechol and o-quinone, generating electrons that amplify UCNPs luminescence. Under optimized conditions, the limit of detection of this biosensor for catechol was 2.7 × 10 −9 M (S/N = 3), detection range of 0.1 × 10 −6 –100 × 10 −6 M. The platform demonstrates high sensitivity, stability, and selectivity, and has been validated in water samples, offering a robust strategy for enzymatic ECL-based environmental monitoring.
研究领域:
分析化学
·
环境科学
·
生物化学
·
纳米技术
·
材料科学
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产品关联
DOI
高影响因子
ELECTROCHIMICA ACTA
·
2025
·
IF 5.6
Xiangrong Zeng,Hongyu Xiao,Yaxuan Lei,Sifei Ye,Peng Liu,Zhuohua Kong,Yangping Wen,Guixia Tan,Xia Gong,Mingfang Li
The simultaneous electrochemical detection of catechol (CC) and hydroquinone (HQ) isomers is hindered by overlapping voltammetric signals, creating a persistent sensitivity-selectivity-accuracy paradox. Herein, we present a synergistic sensing platform integrating BC-Co@NC nanoarchitectures (engineered via topotactic carbonization to form 3D hierarchical pores with atomically dispersed Co-N 4 sites) and a first- and second-derivative voltammetry cascade (FS-DVC) optimized by Savitzky-Golay algorithm. This dual-engine strategy achieves 152 mV interpeak separation, ultra-low limits of detection (0.09 nM for CC, 0.12 nM for HQ), a linear range spanning four orders of magnitude (0.3-500 μM), and robust performance in environmental matrices (95.2-99.1% recoveries in agricultural water/soil). This work establishes a new paradigm for electrochemical isomer discrimination through the synergistic fusion of tailored material design and advanced signal mathematics.
研究领域:
电化学
·
纳米材料
·
分析化学
·
信号处理
·
环境监测
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Hongping Wan,Yunhan Yang,Zhiwen Tu,Mingrun Tang,Bo Jing,Yumei Feng,Jiulong Xie,Huile Gao,Xu Song,Xinghong Zhao
In mucosal vaccination, the targeted delivery of antigens through M (microfold) cells is essential for initiating a robust antigen-specific immune response. In the present study, we devised a nano-delivery platform to target M cells. This platform involved coating mesoporous silica nanoparticles (MSN) with a mucoadhesive chitosan-catechol (Chic) layer, incorporating a recombinant antigen to form nanoparticles that enhance the immune response. The collagenase equivalent domain (COE) of porcine epidemic diarrhea virus (PEDV) terminated with the M cell-targeting sequence RGD (COER), was initially expressed by Escherichia coli ( E. coli ) and subsequently conjugated to the surface of MSN-Chic, forming the MSN-Chic-COER nanoparticles. MSN-Chic-COER with strong mucoadhesive properties and a propensity for M cell targeting, demonstrated enhanced uptake by dendritic cells (DCs) and trafficking to lymph nodes, compared to COE/COER after intranasal administration. MSN-Chic-COER recruited more dendritic cells to the antigen-located site via stimulating chemokine CCL20 secretion was evidenced by cell co-culture model. Additionally, it enhanced antigen permeability by disrupting the distribution of the ZO-1 protein in epithelial cells. Notably, MSN-Chic-COER elicited a higher level of cellular immunity, humoral immunity, and PEDV neutralizing antibody production. These findings underscore the potential of MSN-Chic-COER as a promising intranasal vaccine delivery system.
研究领域:
免疫学
·
疫苗学
·
生物材料科学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 25
·期 18
·9829
·
IF 4.9
Tingfei Chen,Chao Liu,Xiaojun Liu,Chunnan Zhu,Dongyun Zheng
Effectively detecting catechol (CC) and hydroquinone (HQ) simultaneously is crucial for environmental protection and human health monitoring. In the study presented herein, a novel electrochemical sensor for the sensitive simultaneous detection of CC and HQ was constructed based on an electrochemically reduced graphene oxide (ERGO)-modified multi-walled carbon nanotube paste electrode (MWCNTPE). Scanning electron microscopy, X-ray photoelectron spectroscopy, Raman spectroscopy and electrochemical techniques were utilized to characterize the sensing interface and investigate the sensing mechanism. Under the optimal detection conditions, the oxidation peak currents of CC and HQ show a good linear relationship with their concentrations in the range of 0.4–400 μM with a detection limit of 0.083 μM for CC and 0.028 μM for HQ (S/N = 3). Moreover, the sensor exhibits good performance and can be applied successfully in the simultaneous detection of CC and HQ in tap water samples and urine samples with satisfactory results, indicating its promising application prospects.
研究领域:
环境科学
·
分析化学
·
材料科学
·
电化学
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2012
·Vol. 138
·期 4
·1141-1148
·
IF 4.23
Pingping Wang,Yongnian Ni,Serge Kokot
A novel biosensor consisting of a glassy carbon electrode (GCE) modified by a polydiphenylamine-4-sulfonic acid (PDPASA, conjugated polymer) film and double-stranded DNA (dsDNA), i.e. dsDNA/PDPASA/GCE, was researched and developed for the analysis of catechol – a potentially toxic substance for humans and the environment. The surface properties of the PDPASA film, particularly after dsDNA was immobilized on it, were characterized with the use of atomic force microscopy (AFM), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The surfaces of the novel DNA/PDPASA/GCE biosensor changed during the fabrication process and displayed high sensitivity for catechol. The oxidation potential of catechol decreased significantly and the corresponding current increased substantially as compared with the values obtained at the GCE alone and at the dsDNA/GCE. Also, with the addition of hydroquinone, two well discriminated CV peaks were obtained, and it was demonstrated that hydroquinone did not interfere with catechol. DPV analysis produced a linear catechol calibration (range: 0.750 to 8.25 × 10−6 mol L−1; detection limit: 6.48 × 10−7 mol L−1), and thus, various water samples were analysed successfully by this novel method. In addition, the DNA/PDPASA/GCE was used to study DNA damage in the presence of catechol with the use of the Co(phen)33+ electroactive probe. Results indicated that the potentially toxic catechol and its metabolites were all responsible for DNA damage.
研究领域:
生物化学
·
环境科学
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医学
✓ 已验证
DOI
PubMed
高影响因子
COMPOSITES COMMUNICATIONS
·
2020
·
IF 4.915
Dan Yang,Liyuan Yu,Jia Ai,Qungui Wei,Yufeng Ni,Liqun Zhang
With the miniaturization, integration, and functionalization of the electronic components, heat dissipation in the electronic devices has become a challenging issue. Owing to high polarity, carboxyl nitrile butadiene rubber (XNBR) is usually employed as a dielectric material in the electronic devices. However, low thermal conductivity of XNBR hardly satisfies the increasingly stricter demands for heat dissipation. Herein, we used low-cost poly(catechol/polyamine) (PCPA) to modify Al 2 O 3 nanoparticles via biomimetic method for enhancing the interfacial interaction between the Al 2 O 3 filler and XNBR. The ortho-polyphenols in catechol were oxidized into ortho-quinone groups in the alkaline solution. Then, the ortho-quinone groups formed a cross-linked structure to obtain PCPA via the Michael addition or Schiff base reaction with amine. The process decreased the interfacial thermal resistance and phonon scattering, resulting in an enhanced thermal conductivity. When the Al 2 O 3 -PCPA nanoparticles content was 30 vol%, the thermal conductivity of XNBR based composites reached up to 0.51 W/mK, which was 3.19 times that of pure XNBR (0.16 W/mK). The proposed method has potential applications in large scale production of thermally conductive composites in industries, such as electronic packaging materials and thermal management materials.
研究领域:
材料科学
·
高分子化学
·
热管理材料
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DOI
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2019
·Vol. 166
·期 14
·B1293
·
IF 3.12
Yu Zhang,Qiang Xie,Zhi Xia,Guofeng Gui,Feng Deng
In this paper, a novel nanocomposite fulvic acid reduced graphene oxide (FA-rGO) and Co-phthalocyanine nanorods (CoPcNRs) was prepared and used to construct an electrochemical sensor for simultaneous detection of catechol (CC), hydroquinone (HQ), phenol (PN) and p-nitrophenol (p-NP). FA as reductant used to prepare FA-rGO nanocomposite, which fabricated by hydrothermal method and freeze-drying process. The method is environmentally friendly and realizes the gentle reduction of GO. CoPcNRs were synthesized by surfactant-assisted ultrasonic method. The microstructure and morphologies of the as-prepared materials were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and UV-Vis spectrophotometer. The electrochemical oxidation of CC, HQ, PN and p-NP was investigated using differential pulse voltammetry (DPV). The results show that the FA-rGO/CoPcNRs modified electrode exhibits excellent electrochemical activity toward the simultaneous redox of CC, HQ, PN and p-NP. Under optimal conditions, the linear ranges of CC, HQ, PN and p-NP were 1 μM–650 μM, 2 μM–450 μM, 2 μM–160 μM, 2 μM–140 μM, with 0.3 μM, 0.65 μM, 0.65 μM and 0.65 μM, respectively. In addition, the modified electrode showed good selectivity, sensitivity and stability. The application in real samples was also evaluated, and the recoveries suggesting that FA-rGO/CoPcNRs nanocomposites could be used as an excellent potential reaction platform for the simultaneous determination of CC, HQ, PN and p-NP in environmental samples.
研究领域:
环境科学
·
材料科学
·
分析化学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Zhang Man,Li Sidi,Yuan Xubo,Zhao Jin,Hou Xin
In situ hydrogel has attracted widely attention in hemostasis due to its ability to match irregular defects, but its application is limited by insufficient mechanical strength and long gelation time. Although some specifical in situ chemically cross-linked hydrogels could be fast formed and exhibit high mechanical strength, they unable to absorb blood. Hence their applications were further limited in emergency hemostasis usage. In this study, a robust hydrogel formed by hydration of powders was developed using multiple hydrogen bonds crosslinking. Here, catechol groups modified ε-polylysine (PL-CAT) and polyacrylamide (PAAM) were used to construct the PL-CAT/PAAM hydrogel. This hydrogel could be formed within 7 s to adhere and seal bleeding sites. The catechol groups endowed the hydrogel outstanding adhesive strength, which was 3.5 times of fibrin glue. Besides, the mechanical performance of in-situ PL-CAT/PAAM hydrogel was explored and the results showed that the hydrogel exhibited high compressive strength (0.47 MPa at 85% strain). Most importantly, the blood loss of wound treated with PL-CAT/PAAM hydrogel powders was 1/7 of untreated group, indicating the hydrogel's excellent hemostatic effect. And the cytotoxicity studies indicated that the PL-CAT/PAAM hydrogel had low toxicity. To summarize, this hydrogel could be a potential hemostatic material in emergency situations.
研究领域:
材料科学
·
生物医学工程
·
止血
·
组织工程
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DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2021
·
IF 7.312
Hou-Ying Ma,Yi-Ning Hu,Hao Yang,Li-Jing Zhu,Gang Wang,Zhi-Xiang Zeng,Ling-Hui Wang
Janus membranes (JMs) with asymmetrical wettability have been fabricated by (co)deposition of dopamine and its catechol derivatives on the hydrophobic substrates for oil/water emulsions separation, while it remains to be desired to simplify the preparation processes. Here, poly(vinylidene fluoride) membranes using catechol and polyethyleneimine as the additives were fabricated by non-solvent phase inversion on the non-woven fabric. Hydrophilic polycatechol and polyethyleneimine (PCCh-PEI) aggregates migrated on the top side of the fabricated membranes, improving their hydrophilicity. Furthermore, the hydrophobic back surface with irregular nano/micropores and lots of PVDF spherulites was gained after peeling off the supporting fabric. As a result, the as-prepared Janus membranes showed excellent emulsion separation, stability, and durability, which can potentially be used for unidirectional liquid collection with lossless transportation. This work opens a new and facile way for the fabrication of JMs with asymmetric wettability toward the versatile separation of oil/water emulsions.
研究领域:
材料科学
·
界面化学
·
环境工程
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2021
·Vol. 413
·期 13
·3541-3550
·
IF 4.157
Li Zimu,Xi Yachao,Zhao Anqi,Jiang Jianming,Li Bing,Yang Xinming,He Jianbo,Li Fang
Chemiluminescence (CL) reagent luminol was loaded into the porous structure of cobalt-imidazole metal-organic framework (MOF) ZIF-67 to obtain luminol-functionalized ZIF-67 (luminol@ZIF-67) with CL property. The morphology, composition, CL property, and CL mechanism of luminol@ZIF-67 were carefully investigated. The obtained luminol@ZIF-67 exhibited strong, stable, and visible CL emission that reacted with H 2 O 2 , attributed to the strong catalytic effect of ZIF-67 combined with the shortened diffusion distance between luminol and the catalytic center. The CL intensity of luminol@ZIF-67 was more than 550 times higher than that of luminol. Catechol can effectively quench the CL emission of luminol@ZIF-67 that reacted with H 2 O 2 . Then, a simple paper-based CL imaging detection method was developed for the detection of catechol by using a smartphone as a portable detector. The linear calibration curve of the developed CL assay for catechol ranged from 5 to 100 mg/L with detection limit of 1.1 mg/L (S/N = 3δ). The strong CL emission of luminol@ZIF-67 combined with the effective quench ability of catechol guaranteed high sensitivity of the detection method. The practical application ability of the developed CL assay was tested by the determination of catechol in tea and tap water samples, resulting in acceptable results. This work provides an effective paper-based CL detection method for catechol and enriches the species of the chemiluminescent MOF material. Graphical abstract
研究领域:
分析化学
·
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Polymers
·
2019
·Vol. 11
·期 11
·1832
·
IF 3.164
Weili Peng,Zeping Zhang,Minzhi Rong,Mingqiu Zhang
A kind of core-shell hybrid nanoparticle comprised of a hollow mesoporous silica nanoparticles (HMS) core and a copolymer shell bearing N-(3,4-dihydroxyphenethyl) methacrylamide (DMA) and N-isopropylacrylamide (NIPAM) as responsive moieties was prepared. Moreover, the factors that could impact the surface morphology and hierarchical porous structure were discussed. In the presence of Fe3+, catechol-Fe3+complexes were formed to achieve pH-responsive polymer shell, combining with thermal-sensitiveness of poly(N-isopropylacrylamide). Doxorubicin (DOX) was applied as a model drug and the behaviors of its loading/release behaviors were investigated to prove the idea. The results exhibited a significant drug loading capacity of 8.6% and embed efficiency of 94.6% under 1 mg ml–1DOX/PBS solution. In fact, the loading capacity of drug can be easily improved to as high as 28.0% by increasing the DOX concentration. The vitro cytotoxicity assay also indicated that the as-prepared nanoparticles have no significant cytotoxicity on RAW 264.7 cells. The in vitro experiment showed that the cumulative release of DOX was obviously dependent on the temperature and pH values. This pH/temperature-sensitive hollow mesoporous silica nanosphere is expected to have potential applications in controlled drug release.Keywords:hollow mesoporous silica microspheres;thermo-responsive;pH-responsive;catechol-Fe3+;controlled release
研究领域:
材料科学
·
生物医学工程
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POLYMERS FOR ADVANCED TECHNOLOGIES
·
2022
·Vol. 33
·期 12
·4072-4083
·
IF 3.348
Zhonghang Fang,Qunzhang Tu,Zhiyuan Chen,Xinmin Shen,Ming Pan,Kang Liang,Xuan Yang
To improve interfacial adhesion to rubber matrix, a lower cost surface modification strategy of ultrahigh molecular weight polyethylene (UHMWPE) fiber was proposed by replacing expensive dopamine with the two-component system of catechol/tetraethylenepentamine (CAT/TEPA) and then depositing nano zinc oxide (ZnO NPs). By Michael addition reaction or Schiff base reaction between CAT and TEPA, the CAT/TEPA layer was coated on the fiber surface, and further served as a secondary functional platform to realize ZnO NPs deposition through coordination. The surface morphology, chemical structure and composition, wettability and thermal property of the UHMWPE fibers were investigated to evaluate the process and effect of the surface modification. The results of the single-filament tensile test indicated that the tensile strength of the UHMWPE fibers has no deterioration after the modification treatment. In comparison with the original fiber, the pull-out test showed that the highest interface adhesion between the modified UHMWPE fibers and rubber matrix increased by 80.4%. Additionally, it was found that the appropriate zinc ion concentration played an important role in the modification effect.
研究领域:
材料科学
·
表面化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）