Foods
·
2025
·Vol. 14
·期 9
·1648
·
IF 5.1
Feng Yang,Xin Qi,Yan Chen,Kai Tang,Mengyang Fang,Yanwei Song,Jiufen Liu,Lianming Zhang
In this study, β-cyclodextrin (β-CD) functionalized graphene quantum dots (GQDs) was employed to augment the array of chiral recognition sites, thereby enhancing the affinity of GQDs/β-CD composite for imprinting molecules and realizing heightened chiral selectivity. The incorporation of GQDs/β-CD into the synthesis of molecularly imprinted polymers (MIPs), synergizing with the host-guest inclusion properties of β-CD and the abundant carboxyl groups of GQDs, enhanced the chiral recognition capacity of MIPs materials. Consequently, a novel MIPs/(GQDs/β-CD) sensor with chiral recognition capabilities tailored for D-carnitine was successfully fabricated. The binding mechanism between GQDs/β-CD and D-carnitine was elucidated via Ultraviolet-visible spectroscopy and Fourier transform infrared spectroscopy. The variation in the response signal (ΔI) of the probe molecule exhibited a linear correlation with the logarithm of D-carnitine concentration (lgC) in the range of 1.0 × 10−12mol/L to 1.0 × 10−9mol/L, and the detection limit (3δ/S) was calculated as 2.35 × 10−13mol/L. These results underscore a 7.15-fold enhancement in the selectivity of MIPs/(GQDs/β-CD) sensor for D-carnitine recognition. Moreover, the sensor presented commendable efficacy in real-world scenarios, yielding recovery rates ranging from 98.5% to 103.0% during the determination of D-carnitine content in real samples.
研究领域:
分析化学
·
生物医学工程
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DOI
PubMed
高影响因子
Science China-Life Sciences
·
2024
·
IF 8
Zhang Yinghui,Liu Yadan,Jiang Bing,Chen Lifan,Hu Jie,Niu Buying,Chang Jie,Fan Zisheng,Zhou Jingyi,Wang Yajie,Teng Dan,Ma Ning,Wang Xiaofeng,Yang Ruirui,Zheng Mingyue,Zhang Sulin
The development of STING inhibitors for the treatment of STING-related inflammatory diseases continues to encounter significant challenges. The activation of STING is a multi-step process that includes binding with cGAMP, self-oligomerization, and translocation from the endoplasmic reticulum to the Golgi apparatus, ultimately inducing the expression of IRF3 and NF-κB-mediated interferons and inflammatory cytokines. It has been demonstrated that disruption of any of these steps can effectively inhibit STING activation. Traditional structure-based drug screening methodologies generally focus on specific binding sites. In this study, a TransformerCPI model based on protein primary sequences and independent of binding sites is employed to identify compounds capable of binding to the STING protein. The natural product Licochalcone D (LicoD) is identified as a potent and selective STING inhibitor. LicoD does not bind to the classical ligand-binding pocket; instead, it covalently modifies the Cys148 residue of STING. This modification inhibits STING oligomerization, consequently suppressing the recruitment of TBK1 and the nuclear translocation of IRF3 and NF-κB. LicoD treatment ameliorates the inflammatory phenotype in Trex1 −1− mice and inhibits the progression of DSS-induced colitis and AOM/DSS-induced colitis-associated colon cancer (CAC). In summary, this study reveals the potential of LicoD in treating STING-driven inflammatory diseases. It also demonstrates the utility of the TransformerCPI model in discovering allosteric compounds beyond the conventional binding pockets.
研究领域:
免疫学
·
药理学
·
分子生物学
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炎症
·
药物发现
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DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2024
·
IF 6.3
Mengtian Cui,He Wang,Xia Fan,Jianhao Zhang,Changrui Xing,Wenjing Yan
The widespread use of organophosphorus pesticides (OPPs) in foods, poses a serious threat to human and environmental health, it is crucial to develop methods that can efficiently degrade residual OPPs . Herein, a chiral TiO 2 photocatalyst was developed by modification TiO 2 with D-cys/Au NPs (DAT) for the simultaneous degradation of four OPPs (chlorpyrifos, chlorpyrifos-methyl, malathion, and parathion) by natural light. The influence of D-Au NPs concentration on the degradation process was optimized, along with the initial pesticide concentration. Under optimal conditions, the photocatalytic degradation efficiencies of DAT for four OPPs were 96.7 %, 56.9 %, 42.6 %, and 49.2 %, respectively, after 12 h of natural light exposure. 12 degradation products of OPPs were identified, among which three products were identified for the first time. The degradation pathway revealed that the degradation of OPPs by DAT involves the oxidation of the P═S bond and the cleavage of the P-O bond and C-O bond. This study establishes a theoretical foundation for applying chiral TiO 2 nanomaterials for the photocatalytic degradation of OPPs in foods.
研究领域:
环境科学
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食品科学
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材料科学
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DOI
高影响因子
POLYMER DEGRADATION AND STABILITY
·
2024
·
IF 6.3
Jinke Yu,Ling Sun,Lan Ding,Yufei Cao,Xiaohui Liu,Yuanlin Ren,Yuesheng Li
When confronted with a fire, the flammability of rigid polyurethane foam (RPUF) makes it extremely easy to burn violently. In this work, a green biomass derived flame retardant coating was synthesized with phytic acid as acid source, D-sorbitol as carbon source and glycine as gas source. A simple UV curing technique was used to improve the flame retardancy of RPUF . Coated RPUF exhibited a limiting oxygen index value of up to 39.7 % and successfully met UL-94 testing criteria with V-0 rating. In comparison to control RPUF, coated RPUF showed an extended ignition time of 3 s, a remarkable reduction of approximately 60 % in the peak heat release rate, and possessed commendable smoke suppression property. The flame retardant mechanism was analyzed, unveiling the emergence of compact char residue on the surface of coated RPUF after burning, accompanied by the liberation of non-combustible gases. Therefore, these results demonstrated that cost-effective UV-curable flame retardant coating forms protective char layer on the surface of RPUF, providing efficient flame retardancy while preserving its excellent properties.
研究领域:
材料科学
·
化学工程
·
环境科学
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DOI
高影响因子
Small
·
2024
·Vol. 20
·期 10
·2302943
·
IF 13
Yanfen Wan,Yingfei Hu,Hongyu Tu,Wenbo Zhuang,Xuemin Geng,Tian Zhang,Jinghao Zhang,Ming Wen,Peng Yang
Herein, an integrated solar-thermal-power protocol is presented at a micro-nanoscopic level to maximize the energy utilization efficiency involving utilization period and utilization patterns, and the nexus of freshwater production and nanogeneration is realized. This sophisticated vaporization device is constructed with the merits of thermally confined evaporation space in favor of recycling latent heat of condensation and optimizing light absorption based on the local sunlight angle of incidence. Inspired by a bird's nest, Sb 2 WO 6 /D-Fructose composites are prepared as photothermal absorbers to achieve a superior water evaporation rate of 2.78 kg m −2 h −1 in the Multi-stage evaporator. In addition, a synergistic tandem photo thermal-electric device with a combination of solar-driven water evaporation and further waterflow-driven hydrovoltaic generation, which can output a stable voltage of up to 360.8 mV with effective utilization of steam energy and a limited water source, is exploited. Such integrated configurations pave a pathway for clean water production and renewable power generation simultaneously toward energy issues.
研究领域:
能源材料科学
·
环境科学
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2018
·
IF 6.042
Xiaohua Zhu,Lin Fan,Shigong Wang,Chunyang Lei,Yan Huang,Zhou Nie,Shouzhuo Yao
As one of the emerging inorganic graphene analogues, two-dimensional titanium carbide (Ti3C2) nanosheets have attracted extensive attention in recent years because of their remarkable structural and electronic properties. Herein, a sensitive and selective nanoprobe to fluorescently probe phospholipase D activity was developed on the basis of an ultrathin Ti3C2 nanosheets-mediated fluorescence quenching effect. Ultrathin Ti3C2 nanosheets with ∼1.3 nm in thickness were synthesized from bulk Ti3AlC2 powder by a two-step exfoliation procedure and further modified by a natural phospholipid that is doped with rhodamine B-labeled phospholipid (RhB-PL-Ti3C2). The close proximity between RhB and Ti3C2 leads to efficient fluorescence quenching (>95%) of RhB by energy transfer. Phospholipase D-catalyzed lipolysis of the phosphodiester bond in RhB-PL results in RhB moving away from the surface of Ti3C2 nanosheets and subsequent fluorescence recovery of RhB, providing a fluorescent “switch-on” assay for the phospholipase D activity. The proposed nanoprobe was successfully applied to quantitatively determine phospholipase D activity with a low limit of detection (0.10 U L–1) and to measure its inhibition. Moreover, in situ monitoring and imaging the activity of phospholipase D in living cells were achieved using this biocompatible nanoprobe. These results reveal that Ti3C2 nanosheets-based probes exhibit great potential in fluorometric assay and clinical diagnostic applications.
研究领域:
生物化学
·
纳米技术
·
分析化学
·
分子生物学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2018
·
IF 2.88
Yanmei Zhao,Haiyan Yuan,Xinling Zhang,Jidong Yang
An activatable fluorescence monitoring platform based on a novel Maillard reaction product from d -glucose and L-arginine was prepared through a facile one-pot approach and applied for simultaneous detection of d -isoascorbic acid and tartaric acid . In this work, the new Maillard reaction product GLA was first obtained, and its fluorescence intensity can be effectively quenched by KMnO 4 , resulting from a new complex (GLA-KMnO 4 ) formation between GLA and KMnO 4 . Upon addition of d -isoascorbic acid or tartaric acid, an enhanced fluorescence was observed under the optimumed experimental conditions, indicating a stimuli-responsive fluorescence turn on platform for d -isoascorbic acid or tartaric acid can be developed. The corresponding experimental results showed that this turn on fluorescence sensing platform has a high sensitivity for d -isoascorbic acid or tartaric acid, because the detection limits were 5.9 μM and 21.5 μM, respectively. Additionally, this proposed sensing platform was applied to simultaneously detection of d -isoascorbic acid and tartaric acid in real tap water samples with satisfactory results.
研究领域:
分析化学
·
食品科学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Mubalake Rehemaitijiang,Gaosheng Li,Rongchao Zhu,Baiyi Zu,Xincun Dou,Zhi Su,Zhenzhen Cai
The rational design of the D-π-A chemodosimeter with a significant Stokes shift is of great importance for enhancing the visualization of optical sensing signals. Here, three D-π-A fluorescent chemodosimeters with 2-(3-cyano-4,5,5-trimethylfuran-2(5H)-ylidene) malononitrile (TCF) as the electron-withdrawing group are synthesized by precisely modulating the electron-releasing strength. By decreasing the ability of electron release, the electrophilicity of the recognition site is increased by 1.449 kcal/mol, the Stokes shift of the chemodosimeter is improved to 201 nm, and the sensing mode changes from fluorescence quenching to ratiometric fluorescence and finally to fluorescence on. Furthermore, the three D-π-A fluorescent chemodosimeters display superior sensing performance toward ClO–, including low limits of detection (LOD, 37.0, 5.1, and 1.0 nM), rapid response (<5 s), and great selectivity in the presence of 16 kinds of interferents. Moreover, the practicality of the chemodosimeters is further validated by a portable triple-standard quantitative array detection platform, which can quantitatively detect ClO– solutions. The proposed design and modulation strategy for chemodosimeters can provide a new pathway for the sensitive and visualized identification of oxidants and other hazardous chemicals.
研究领域:
分析化学
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生物医学工程
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DOI
PubMed
高影响因子
IMMUNITY
·
2025
·
IF 26.3
Qianzhou Yu,Danlu Jiang,Tianming Zhao,Yuelin Guan,Jian Zhang,Dehang Yang,Ruya Sun,Weiwei Yu,Zhen Wang,Sheng Chen,Mobai Li,Tianyi Hu,Qiqi Deng,Xiaoyang Lu,Yidong Yang,Mengfei Chang,Liheng Du,Xue Zhang,Zhexu Chi,Di Wang
The small intestine (SI) absorbs nutrients and acts as a barrier against pathogens. Diet enables the absorptive function of the SI while maintaining immune homeostasis. But how the SI transmits nutritional signals to the immune response and adapts to dietary intake remains unclear. Here, we demonstrated that epithelial gasdermin D (GSDMD) in the SI facilitated the absorptive function of enterocytes, subsequently empowering an epithelial-immune cooperation to modulate host defense. Unlike its pyroptotic function, GSDMD determines the absorptive versus the defensive zonation of enterocytes and promotes brush border assembly. This diet-induced, GSDMD-mediated adaptation promoted lipid absorption and subsequently rewired enterocyte metabolism to support intraepithelial γδ T lymphocytes (γδ T-IELs), thereby enhancing barrier function. Impairment of this GSDMD-mediated circuit exacerbated barrier-dysfunction-associated enteritis. Our results reveal how epithelial cells and lymphocytes co-adapt to nutrient signals in the SI, thereby adjusting the equilibrium between nutrient uptake and host defense in response to environmental change.
研究领域:
免疫学
·
细胞生物学
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营养学
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胃肠病学
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分子生物学
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DOI
PubMed
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 5
·1181
·
IF 4.2
Minghe Wang,Zhouyuji Liao,Dongting Zhangsun,Yong Wu,Sulan Luo
Antimicrobial peptides (AMPs), acknowledged as host defense peptides, constitute a category of predominant cationic peptides prevalent in diverse life forms. This study explored the antibacterial activity of α-conotoxin RgIA, and to enhance its stability and efficacy, D-amino acid substitution was employed, resulting in the synthesis of nine RgIA mutant analogs. Results revealed that several modified RgIA mutants displayed inhibitory efficacy against various pathogenic bacteria and fungi, includingCandida tropicalisandEscherichia coli. Mechanistic investigations elucidated that these polypeptides achieved antibacterial effects through the disruption of bacterial cell membranes. The study further assessed the designed peptides’ hemolytic activity, cytotoxicity, and safety. Mutants with antibacterial activity exhibited lower hemolytic activity and cytotoxicity, with Pep 8 demonstrating favorable safety in mice. RgIA mutants incorporating D-amino acids exhibited notable stability and adaptability, sustaining antibacterial properties across diverse environmental conditions. This research underscores the potential of the peptide to advance innovative oral antibiotics, offering a novel approach to address bacterial infections.
研究领域:
药理学
·
微生物学
·
生物化学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 11
·1-11
·
IF 5.3
Lu Feng,Li Limao,Shen Kang,Qian Yayun,Zhang Pengfei,Yang Yan,Zhu Qunshan,Huang Yong,Yan Chunxiang,Wei Wei
A Raman-active boronate modified surface-enhanced Raman scattering (SERS) microporous array chip based on the enzymatic reaction was constructed for reliable, sensitive, and quantitative monitoring of D-Proline (D-Pro) and D-Alanine (D-Ala) in saliva. Initially, 3-mercaptophenylboronic acid (3-MPBA) was bonded to Au-coated Si nanocrown arrays (Au/SiNCA) via Au–S bonding. Following this, H 2 O 2 obtained from D-amino acid oxidase (DAAO)-specific catalyzed D-amino acids (D-AAs) further reduced 3-MPBA to 3-hydroxythiophenol (3-HTP) with a new Raman peak at 882 cm −1 . Meanwhile, the original characteristic peak at 998 cm −1 remained unchanged. Therefore, the I 882 /I 998 ratio increased with increasing content of D-AAs in the sample to be tested, allowing D-AAs to be quantitatively detected. The Au/SiNCA with large-area periodic crown structure prepared provided numerous, uniform “hot spots,” and the microporous array chip with 16 detection units was employed as the platform for SERS analysis, realizing high-throughput, high sensitivity, high specificity and high-reliability quantitative detection of D-AAs (D-Pro and D-Ala). The limits of detection (LOD) were down to 10.1 µM and 13.7 µM throughout the linear range of 20–500 µM. The good results of the saliva detection suggested that this SERS sensor could rapidly differentiate between early-stage gastric cancer patients and healthy individuals. Graphical Abstract
研究领域:
生物化学
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医学诊断
·
分析化学
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DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL THERMODYNAMICS
·
2018
·
IF 2.631
Xiao Feng,Ali Farajtabar,Hu Lin,Gaoquan Chen,Zhiying Wang,Xinbao Li,Hongkun Zhao
The D-tryptophan solubility in aqueous solutions of methanol, ethanol, isopropanol and N , N -dimethylformamide (DMF) was measured by the saturation shake-flask method at temperatures ranging from 283.15 K to 333.15 K. The maximum solubility was found in water for alcohol (1) + water (2) mixtures, and in neat DMF for DMF (1) + water (2) mixture. When the same temperature and mass fraction of the water is given, the highest mole fraction solubility of D-tryptophan was found in (DMF + water). The solid phases were tested by X-ray powder diffraction (XPRD) showing no polymorphic transformation, solvate formation or crystal transition. Three solubility models, i.e. the Jouyban-Acree, van’t Hoff-Jouyban-Acree and Apelblat-Jouyban-Acree were employed to correlate the experimental solubility values so as to obtain thermodynamic parameters. The RAD and RMSD data were no more than 1.65% and 9.40 × 10 −5 , respectively. Linear solvation energy relationship analysis based on Kamlet-Taft parameters indicated that the variation of D-tryptophan solubility depended on π ∗ for methanol (ethanol, isopropanol) (1) + water (2), and cavity term for DMF (1) + water (2). The preferential solvation parameters ( δx 1,3 ) for methanol, ethanol, isopropanol and DMF derived by means of the inverse Kirkwood–Buff integrals were negative in the four solvent mixtures with intermediate and alcohol-rich compositions, which indicated that D-tryptophan was preferentially solvated by water. The higher solvation by water could be explained in terms of the higher acidic behaviour of the solvents interacting with the Lewis basic groups of the D-tryptophan. However D-tryptophan is preferentially solvated neither by water nor by DMF in DMF + water mixture.
研究领域:
化学
·
生物学
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DOI
高影响因子
危险品化学品经营许可证（带存储）