COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2021
·
IF 4.539
Peizhang Zhou,Yuanyang Yan,Jiang Cheng,Cailong Zhou
In this work, stainless steel mesh coated with stearic acid and tetradecanedioic acid modulated ZIF-67 (SA+TDA/mZIF-67@SSM) was prepared via facile electrodeposition . The composite modulators supply defect engineering on the MOFs , endowing the as-prepared meshes with pH-switchable wettability . The ratio of modulators significantly affected the crystal morphology and wetting property of SA+TDA/mZIF-67@SSM, resulting from the competitive coordination between the modulation and crystal growth process. The SA+TDA/mZIF-67@SSM exhibited highly hydrophobic, while it became amphiphilic after soaked in alkaline solution because some exposed carboxylic acid groups on the mesh were deprotonated. The SA+TDA/mZIF-67@SSM achieved efficient and adjustable organic liquids separation, with the separation efficiency both up to 98% before and after the wettability transition. In addition, the SA+TDA/mZIF-67@SSM could realize the separation of oil/water/oil mixtures, with the separation efficiency larger than 98%. This work introduces a new technique of modulated MOFs coated mesh with pH-responsivity for separating organic liquids based on defect engineering.
研究领域:
材料科学
·
化学工程
✓ 已验证
DOI
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2025
·
IF 4.9
Ahmed M. Omer,Eman M. Abd El-Monaem,Heba S. Hassan,Mahmoud A. Elmeligy,Angela Kleinová,Abdelazeem S. Eltaweil,Abolfazl Heydari
pH-sensitive carriers have emerged as an optimal choice for the oral administration of anticancer drugs, exhibiting the desired release performance. This study aims to develop biocomposite beads for the sustained release of the anticancer drug 5-fluorouracil (5-FU). Here, 5-FU was encapsulated within a ZIF-8 metal-organic framework (MOF), which was subsequently impregnated into amino-chitosan beads. The resulting Am-CS@ZIF-8 biocomposite beads were characterized for their chemical structure, surface morphology, elemental composition, crystallinity, surface charges, and mechanical properties utilizing Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), zeta potential analysis, and a texture analyzer, respectively. The developed beads exhibited a textured surface, denser composition, and a positively charged surface with a maximum zeta potential of +12.4 at pH 7.4. In comparison to Am-CS beads, the Am-CS@ZIF-8 biocomposite beads demonstrated superior mechanical properties, achieving a maximum stress value of 5.668 MPa compared to the 1.874 MPa recorded for pure Am-CS beads. pH sensitivity was assessed through the investigation of water uptake behavior at pH 1.2 and pH 7.4. The Am-CS@ZIF-8 biocomposite beads demonstrated a high encapsulation efficiency of approximately 81.6 % for 5-FU. They exhibited pH-responsive release behavior, with only 17 % of the drug released at pH 1.2 after 2 h and a sustained release of 52 % at pH 7.4 over 24 h. The release mechanism was predominantly governed by Fickian diffusion, as closely fitted by the Peppas-Sahlin kinetic model. The formulated carrier confirmed its biodegradability under enzymatic physiological conditions, suggesting its potential applicability as an effective system for the oral delivery of anticancer drugs.
研究领域:
药剂学
·
生物材料学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Qingqing Yu,Hui Hong,Yueyue Liu,Abdul Razak Monto,Ruichang Gao,Yulong Bao
The pH buffering capacity is an important functionality of muscle proteins, and muscle foods are susceptible to being oxidized during storage and processing. In order to study the effect of oxidation on the pH buffering capacity of myofibrillar proteins, myofibrils extracted from snakehead fish ( Channa argus ) were oxidized with H 2 O 2 . Results showed that increased oxidation led to loss of free sulfhydryl groups, formation of carbonyl groups, increased surface hydrophobicity , and aggregation of myofibrillar proteins. In addition, there was a significant reduction in the content of histidine in oxidized myofibrillar proteins. The pH buffering capacity of myofibrillar proteins significantly decreased from 3.14 ± 0.03 mM H + /(mL × ΔpH) down to 2.55 ± 0.03 mM H + /(mL × ΔpH) after oxidation with 50 mM H 2 O 2 . Both oxidized myofibrillar proteins and histidine showed a high pH buffering capacity at pH near 5.8, which is the histidine pKa value. Here, we hypothesize that oxidation-induced changes in the pH buffering capacity of myofibrillar proteins were driven by oxidative modification of histidine and structural changes of myofibrillar proteins. The significance of this study to food industry may be the awareness that protein oxidation may affect pH through changes in buffering capacity. And the use of antioxidants, especially those targeting at histidine will be promising in addressing this issue.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Kangming Xiong,Caixia Yin,Yongkang Yue,Fangjun Huo
The intracellular pH has a significant effect on several essential biological processes such as material transfer, enzymatic action, cell apoptosis et al. Thus, it is necessary to monitor pH fluctuation in living cells. Here, we designed a near-infrared ratiometric fluorescence probe for pH detection. The spectroscopic responses of probe to pH variations were investigated in CH 3 OH/PBS (v/v, 1:1) mixed solution at different pH values. The experimental results showed that the probe is sensitive to acidity, and the p K a of probe is calculated to be 4.85. When the pH was decreased from 9.0 to 1.0, the color of probe solution change from purple to yellow was found by naked eye. Moreover, the probe can be a practical tool for assessing cellular pH by cell imaging.
研究领域:
生物化学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2019
·Vol. 7
·期 45
·7129-7140
·
IF 5.047
Jing Qu,Rui Wang,Si Peng,Mengyao Shi,Sheng-Tao Yang,Jian-bin Luo,Juan Lin,Qing-han Zhou
The systemic toxicity, reduced cellular internalization, and uncontrollable intracellular drug release of smart nanoparticles (NPs) still need to be overcome for effective cancer therapy. Herein, a series of stepwise dual pH and redox responsive cross-linked polypeptides based on poly(L-lysine-co-N,N-bis(acryloyl)cystamine-co-γ-glutamic acid) (PLBG), were prepared for enhanced cellular uptake and effective cancer therapy. The prepared cross-linked PLBG nanoparticles (PLBG-NPs) exhibit negatively charged surfaces under physiological conditions, and the surface charge of the PLBG-NPs was observed to switch from negative to positive in a slightly acidic tumor extracellular environment at a pH level of ∼6.5. Finally, the endosome escape of the PLBG-NPs was facilitated via a “proton-sponge” effect via protonation of the polymer chain in the endo/lysosome environment of the tumor cell at a pH level of ∼5.0. Moreover, rapid drug release was triggered by a high concentration of reducing glutathione (GSH) in tumor cells via destruction of the disulfide linkages in the PLBG-NPs. Cytotoxicity assays demonstrated that no noticeable cytotoxicity was observed for the PLBG-NPs. However, DOX-loaded PLBG-NPs (PLBG-NPs-DOX) demonstrated remarkable tumor cell killing effects. In HeLa tumor-bearing mice, PLBG-NPs-DOX showed sustained tumor accumulation and enhanced inhibitory effects on tumor growth and angiogenesis. In conclusion, it is suggested that the as-designed novel charge-conversion PLBG-NPs with stepwise pH-responsivity and biodegradability could be used as a potential drug carrier for effective cancer therapy with less systemic toxicity.
研究领域:
生物医学工程
·
肿瘤学
·
药剂学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2019
·
IF 7.65
Suding Yan,Xinping Zhang,Hui Zhang
The combination of persulfate (PS) activation by iron ions with electrochemical process (electro/Fe 3+ /PS) is a promising advanced oxidation process. However, almost all these systems were performed in an unbuffered solution and actually under acidic pH condition, with the electricity being frequently supplied by external power. Considering the high buffering capacity of wastewater and energy saving, peroxydisulfate (PDS) activation by Fe(III) species with bioelectricity provided by microbial fuel cell (MFC) for bisphenol A (BPA) oxidation was investigated at fixed near-neutral pH as well as acidic pH. The results indicate that 90.8% of BPA could be removed at pH 2.5. Though the iron existed in the form of precipitate, BPA could still be efficiently removed at pH 6.0. The precipitate formed in the system at pH 6.0 was identified as the amorphous iron oxyhydroxides. Sulfate radicals in the bulk solution and that adsorbed on the precipitate were the dominant reactive species responsible for the oxidation of BPA in the homogeneous and heterogeneous MFC/Fe(III)/PDS processes, respectively. The mechanisms of BPA degradation at both pH values were proposed via EPR and quenching tests as well as XPS analysis. The effects of operating parameters, the mineralization, the mineralization current efficiency and energy consumption were also explored.
研究领域:
环境科学
·
化学工程
✓ 已验证
DOI
高影响因子
POLYMER
·
2020
·
IF 4.231
Kun Yu,Xiyao Yang,Lihua He,Rong Zheng,Jie Min,Hongying Su,Shaoyun Shan,Qingming Jia
Dual and multi-stimuli responsive polymeric nanoparticles that can respond to two or more signals have been demonstrated as prospective drug carriers with enhanced tumor accumulation and on-demand drug release profiles. In this study, dextran-based (Dex-SS) nanogels were developed via a facile method based on the disulfide containing Schiff base formation between polyaldehyde dextran and cystamine in water-in-oil inverse microemulsion . Acidic and reductive (GSH) environment sensitive degradation behaviors of the resulted nanogels were investigated by morphology analysis of the treated nanogels using SEM imaging. Doxorubicin (DOX) was covalently conjugated into the dextran nanogels via Schiff base linkages, and pH/GSH dual sensitive drug release profiles were demonstrated. Effective cell uptake of the DOX-loaded nanogels was finally identified by human cancer cell line of H1299. Take advantage of the acidic and reductive tumor microenvironment, the dual-stimuli responsive Dex-SS nanogels can serve as microenvironment-sensitive drug delivery systems for tumor therapy.
研究领域:
药剂学
·
生物材料学
·
肿瘤学
✓ 已验证
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2015
·
IF 2.752
Sha Jin,Dian Li,Peng Yang,Jia Guo,Jennifer Q. Lu,Changchun Wang
Stimuli responsive drug delivery systems have attracted considerable attention due to their ability to deliver drugs on demand to special tissues and cells (e.g. cancer cells). In this paper, we fabricated a new kind of redox/pH dual stimuli-responsive biodegradable poly(methacrylic acid) (MAA) nanohydrogels crosslinked by N , N -bis(acryloyl)cystamine (BACy) through a facile reflux-precipitation polymerization. Subsequently polyethylene glycol (PEG) was grafted onto the surface of nanohydrogels to improve their long circulation time. This kind of PEGylated nanohydrogels could be degraded into short linear polymer chains in the solution of 10 mM glutathione (GSH) due to the sensitive disulfide bonds in the cross-linked networks. Interestingly, we found that the hydrophilic PEGylated nanohydrogels could be simultaneously loaded with hydrophilic drug doxorubicin (DOX) and hydrophobic drug paclitaxel (PTX) with high drug loading efficiency because of its regional hydrophobic nature in the nanohydrogels. The cumulative release profile showed that either lower pH (5.0) or reducing environment (GSH) or a combination could significantly accelerate the drug release from the drug-loaded nanohydrogels. In addition, the experimental results on living cells indicated that the multi-drug-loaded system had synergistic effect on cancer therapy.
研究领域:
药剂学
·
生物材料
·
肿瘤学
✓ 已验证
DOI
高影响因子
ANALYST
·
2016
·Vol. 141
·期 15
·4766-4771
·
IF 4.033
Jingni Fu,Changqin Ding,Anwei Zhu,Yang Tian
Intracellular pH plays a vital role in cell biology, including signal transduction, ion transport and homeostasis. Herein, a ratiometric fluorescent silica probe was developed to detect intracellular pH values. The pH sensitive dye fluorescein isothiocyanate isomer I (FITC), emitting green fluorescence, was hybridized with reference dye rhodamine B (RB), emitting red fluorescence, as a dual-emission fluorophore, in which RB was embedded in a silica core of ∼40 nm diameter. Moreover, to prevent fluorescence resonance energy transfer between FITC and RB, FITC was grafted onto the surface of core–shell silica colloidal particles with a shell thickness of 10–12 nm. The nanoprobe exhibited dual emission bands centered at 517 and 570 nm, under single wavelength excitation of 488 nm. RB encapsulated in silica was inert to pH change and only served as reference signals for providing built-in correction to avoid environmental effects. Moreover, FITC (λem = 517 nm) showed high selectivity toward H+ against metal ions and amino acids, leading to fluorescence variation upon pH change. Consequently, variations of the two fluorescence intensities (Fgreen/Fred) resulted in a ratiometric pH fluorescent sensor. The specific nanoprobe showed good linearity with pH variation in the range of 6.0–7.8. It can be noted that the fluorescent silica probe demonstrated good water dispersibility, high stability and low cytotoxicity. Accordingly, imaging and biosensing of pH variation was successfully achieved in HeLa cells.
研究领域:
生物化学
·
细胞生物学
·
纳米材料科学
✓ 已验证
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Zihang Cheng,Kedu Wang,Dongling Qiao,Bowen Li,Siming Zhao,Binjia Zhang
The global aging of the population is accelerating, and dysphagia poses a threat to the health of the elderly. Thickened fluid foods prepared from polysaccharides and proteins could address dehydration and protein deficiency in the elderly dysphagia population. In this work, the structural characteristics, rheological properties, and swallowing characteristics of complexes formed by whey protein isolate (WPI) and polysaccharides (xanthan gum (XG), λ-carrageenan (λC), and konjac glucomannan (KGM)) at different pH values were investigated. As the pH decreased, there was a decrease in the charge of WPI, which weakened the electrostatic interactions resulting in the higher turbidity and particle size of complexes. At near pH 4, the XW complex had a moderate level of yield stress, zero-shear viscosity, and adsorption height, which resulted in moderate oral transit time, stretch length, and residual rate during swallowing, making it more potential as dysphagia foods. However, at pH near 4, λW and KW complexes had lower viscosity, adsorption height, and IDDSI levels, making them less suitable for dysphagia foods. The above results suggest that in WPI-enriched thickened fluids, the pH value could be appropriately adjusted to change the rheological properties and swallowing characteristics of the thickened fluids, thereby producing products adapted to elderly people with dysphagia.
研究领域:
食品科学
·
老年医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2015
·
IF 4.513
Yanyan Huang,Xiang Ran,Youhui Lin,Jinsong Ren,Xiaogang Qu
Based on enzymatic reactions-triggered changes of pH values and biocomputing, a novel and multistage interconnection biological network with multiple easy-detectable signal outputs has been developed. Compared with traditional chemical computing, the enzyme-based biological system could overcome the interference between reactions or the incompatibility of individual computing gates and offer a unique opportunity to assemble multicomponent/multifunctional logic circuitries. Our system included four enzyme inputs: β-galactosidase (β-gal), glucose oxidase (GOx), esterase (Est) and urease (Ur). With the assistance of two signal transducers (gold nanoparticles and acid–base indicators) or pH meter, the outputs of the biological network could be conveniently read by the naked eyes. In contrast to current methods, the approach present here could realize cost-effective, label-free and colorimetric logic operations without complicated instrument. By designing a series of Boolean logic operations, we could logically make judgment of the compositions of the samples on the basis of visual output signals. Our work offered a promising paradigm for future biological computing technology and might be highly useful in future intelligent diagnostics, prodrug activation, smart drug delivery, process control, and electronic applications.
研究领域:
生物化学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2020
·
IF 7.489
Gong Zhang,Liwei Zhang,Hengjun Rao,Yuefei Wang,Qing Li,Wei Qi,Xuejiao Yang,Rongxin Su,Zhimin He
We report the self-assembly of an ultra-pH-sensitive hydrogel from a simple dipeptide derivative, ferrocene-diphenylalanine (noted as Fc-FF). The pure Fc- L -Phe- L -Phe-OH (noted as (L)-Fc-FF) or Fc- D -Phe- D -Phe-OH (noted as (D)-Fc-FF) peptide could self-assemble into right- or left-handed nanohelices in a mixture of water and organic solvent leading to formation of stable chiral hydrogels at a very narrow pH range of 5.7–5.9. Intriguingly, if we used a racemic mixture (noted as (L,D)-Fc-FF) of (L)-Fc-FF and (D)-Fc-FF, stable hydrogels could only be formed at much lower pH values with a weak mechanical property. The results indicated that the molecular chirality has great influence on the gelation of the peptides. Moreover, we investigated the gelation behaviour of the peptides in various solvents. Here the water molecules are essential in directing the chiral self-assembly of Fc-FF into entangled chiral nanostructures, leading to the formation of stimuli-responsive hydrogels. And in the presence of water molecules, a small amount of organic co-solvents also has significant effect on the gelation of Fc-FF peptides. This work demonstrated that the gelation of such a bioorganometallic molecule is very sensitive to the molecular conformation of itself and the self-assembly environment (pH, solvents, etc.), which has potential applications in chemical sensing and biomedicine.
研究领域:
生物化学
·
材料科学
·
纳米技术
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）