Biomedical Materials
·
2023
·Vol. 18
·期 4
·045007
·
IF 4
Yanwei Li,Susu Wang,Yurong Li,Guozheng Zhang,Tangfeng Wu,Yongkang Wei,Xinyu Cao,Hui Yan,Peisheng Liang,Zihan Yan,Yanan Guo,Yeshun Zhang
Wound repair is challenging for traditional wound dressings. New bioactive dressings need to be developed urgently. Herein, we reported a highly bioactive silk protein wound dressing (SPD) with natural silk fiber-sericin hydrogel interpenetrating double network structure, which combines the dual characteristics of natural silk and sericin hydrogel. Silk fiber scaffolds were secreted directly from silkworms bred by regulating their spinning behaviors. Sericin in SPD is obtained by dissolving silkworm cocoons at high temperature and high pressure, while it remains intact activities to self-assemble a hydrogel. To explore the effect of SPD, we first systematically evaluated its physicochemical properties and biological activities in vitro. The SPD exhibits high porosity, prominent mechanical strength, pH-responsive degradability, and excellent anti-oxidation and cell compatibility. Besides, SPD can load and maintain long-term drug release. Based on the satisfactory performance of SPD in vitro, effective in vivo treatment was achieved in a mouse full-thickness wound model, as demonstrated by a significantly accelerated wound healing process, promote the regeneration of hair follicles and sebaceous glands, increased expression of vascular endothelial growth factor, and reduced inflammation. Further, resveratrol was loaded into SPD to enhance the effects of anti-oxidation and anti-inflammation for wound healing. Our investigation shows that SPD with excellent physicochemical and biological properties applied in a murine full-thickness skin wound model resulted in remarkable and efficient acceleration of healing process, which may inspire the design of new, effective, and safer medical materials for tissue regeneration.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·
IF 6.7
Na Li,Zisheng Zhai,Yao Qin,Xusong Wang,Hai Chi,Fawen Yin,Dayong Zhou,Deyang Li
Abstract Antarctic krill protein (AKP) is a high-value marine protein limited by aggregation, low solubility, and poor compatibility with hydrophobic bioactives. Here, we applied ultrasound-assisted inulin glycosylation to prepare AKP–inulin conjugates and AKP–inulin–resveratrol ternary complexes, systematically examining their structure–function relationships. Ultrasound enhanced glycosylation, reaching the highest grafting degree at 480 W, and improved colloidal stability, as indicated by reduced particle size and increased absolute zeta potential. Structural analyses revealed α-helix reduction, increased β-sheet and disordered structures, molecular unfolding, and enhanced cross-linking. The 480 W ternary complex exhibited superior emulsifying activity, high interfacial protein adsorption, improved 10-day storage stability, and enhanced antioxidant capacity, with DPPH scavenging reaching 63.41%. These results demonstrate that ultrasound-assisted glycosylation reconfigures AKP into a resveratrol-containing, interfacially active matrix with improved colloidal and emulsion stability, providing mechanistic insight into protein–polysaccharide interactions and supporting the development of AKP-based ternary complexes for functional food applications.
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JOURNAL OF LIPOSOME RESEARCH
·
2023
·
IF 4.4
Yang Yu,Shutong Li,Liang Kong,Yumeng Du,Yang Liu,Juan Zang,Ruibo Guo,Lu Zhang,Ziyue Zhao,Ruijun Ju,Xuetao Li
As the aging population continues to increase, aging-related inflammation, oxidative stress, and neurodegenerative diseases have become serious global health threats. Resveratrol, a star molecule in natural polyphenols, has been widely reported to have physiological activities such as anti-aging, anti-inflammatory, antioxidant, and neuroprotection. However, its poor water solubility, rapid metabolism, low bioavailability and poor targeting ability, which limits its application. Accordingly, a brain-targeted resveratrol liposome (ANG-RES-LIP) was developed to solve these issues. Experimental results showed that ANG-RES-LIP has a uniform size distribution, good biocompatibility, and a drug encapsulation rate of over 90%. Furthermore, in vitro cell experiments showed that the modification of the targeting ligand ANG significantly increased the capability of RES to cross the BBB and neuronal uptake. Compared with free RES, ANG-RES-LIP demonstrated stronger antioxidant activity and the ability to rescue oxidatively damaged cells from apoptosis. Additionally, ANG-RES-LIP showed the ability to repair damaged neuronal mitochondrial membrane potential. In vivo experiments further demonstrated that ANG-RES-LIP improved cognitive function by reducing oxidative stress and inflammation levels in the brains of aging model mice, repairing damaged neurons and glial cells, and increasing brain-derived neurotrophic factor. In summary, this study not only provides a new method for further development and application of resveratrol but also a promising strategy for preventing and treating age-related neurodegenerative diseases.
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CARBOHYDRATE POLYMERS
·
2021
·
IF 9.381
Peng Wang,Zhi-gang Luo,Zhi-gang Xiao
Hydrogel beads composed of oxidized gellan gum (OGG) and resistant starch (RS) were successfully fabricated by ionic cross-linking and used as delivery carriers for resveratrol. Firstly, OGG with different degrees of oxidation were prepared through 2, 2, 6, 6-tetramethylpiperidine-1-oxyl radical (TEMPO)-mediated oxidation, and characterized by Fourier transform infrared spectroscopy and carbon-13 nuclear magnetic resonance to prove that carboxyl groups were successfully introduced into the gellan gum molecules. Molecular weight, thermal stability, zeta potential and gelation temperature of OGG were also investigated. Subsequently, resveratrol was encapsulated into OGG/RS hydrogel beads in the form of resveratrol/β-cyclodextrins inclusion complexes. The addition of RS significantly influenced the morphological structure and swelling capacity of OGG/RS hydrogel beads. The OGG/RS hydrogel beads exhibited a pH-sensitivity and high encapsulation efficiency of resveratrol (84.95 %–90.73 %). Furthermore, the in-vitro release behaviors demonstrated that OGG/RS hydrogel beads showed good stability in simulated gastric fluids and sustained release of resveratrol in simulated intestinal fluids. The obtained results indicate that OGG/RS hydrogel beads show a potential as delivery system for resveratrol in the food industry.
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FOOD HYDROCOLLOIDS
·
2021
·
IF 9.147
Yizhou Xu,Jiulin Wu,Shaoyun Wang
To overcome hydrophobicity and chemical instability of resveratrol (Rev), modified whey protein isolate (WPI) and gelatin (Gel) were used to load Rev. Three treatments including pH-shifting at pH 12 (S), pH-shifting at pH 12 combined with ultrasonication at pH 7 (SU7), and pH-shifting at pH 12 combined with ultrasonication at pH 12 (SU12) were conducted to obtain a series of modified proteins (WPI-S, WPI-SU7, WPI-SU12, Gel-S, Gel-SU7, Gel-SU12). Among them, WPI-SU12 and Gel-SU12 had the highest encapsulation efficiency (EE) and loading capacity (LC) for Rev. The EE and LC were 92.69 ± 0.25% and 17.37 ± 0.067 g/100 g protein in WPI-SU12-Rev nanocomposite , respectively, and 83.85 ± 0.43% and 15.68 ± 0.070 g/100 g protein in Gel-SU12-Rev nanocomposite, respectively. This might be related to the highest surface hydrophobicity of modified proteins in SU12 groups. The particle sizes of all nanocomposites were below 200 nm, while the higher ζ-potential absolute value of WPI-SU12-Rev nanocomposite than that of Gel-SU12-Rev nanocomposite indicated higher stability. Molecular interaction and secondary structure analysis verified that the proteins and Rev bound to each other. Moreover, protein nanocomposites could greatly improve the water solubility and antioxidant capacity of Rev. In summary, the WPI modified by ultrasonication at pH 12 showed the best loading with Rev.
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APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2020
·Vol. 191
·期 3
·1340-1352
·
IF 2.277
Su Yanbin,Sun Chengyu,Sun Xuwei,Wu Ruixue,Zhang Xing,Tu Yunzhou
The aim of this study was to explore whether or not acetylresveratrol as a potential substitute for resveratrol dragged the toxic aldehyde to inhibit the mutation of mitochondrial DNA. The results revealed that the acetylresveratrol shifted ultraviolet peak of trans -crotonaldehyde from 316 to 311 nm. In mitochondria, the acetylresveratrol split the ultraviolet peak at 311 nm of trans -crotonaldehyde into 311 nm and 309 nm; the aldehyde Raman band of trans -crotonaldehyde was red shifted by the acetylresveratrol from 1689 to 1686 cm −1 with obvious band decline; Raman bands at 1149 cm −1 , 1168 cm −1 , and 1325 cm −1 of acetylresveratrol disappeared. In aldehyde dehydrogenase, the aldehyde Raman band of trans- crotonaldehyde was red shifted by the acetylresveratrol from 1689 to 1684 cm −1 with band decline; Raman bands at 1150 cm −1 , 1168 cm −1 , and 1324 cm −1 of acetylresveratrol declined. The weak acidic microenvironment was the best, for the acetylresveratrol dragged the toxic aldehyde of trans -crotonaldehyde. Compared with the resveratrol, the effect of the acetylresveratrol on the toxic aldehyde of trans -crotonaldehyde was very similar to that of the resveratrol. The acetylresveratrol is very suitable as a potential substitute for resveratrol dragged the toxic aldehyde to inhibit the mutation of mitochondrial DNA.
In mitochondria, the Raman band of the toxic –CH=O of trans- crotonaldehyde (TCA) dragged by the Acetyl-Res from 1689 to 1686 cm −1 with obvious band decline, while the Raman bands at 1149 cm −1 , 1168 cm −1 , and 1325 cm −1 of the Acetyl-Res disappeared, respectively. The Acetyl-Res is very suitable as a potential substitute, for the Res dragged the toxic –CH=O of TCA to inhibit the mutation of mitochondrial DNA for anticancer.
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JOURNAL OF ANALYTICAL CHEMISTRY
·
2020
·Vol. 75
·期 1
·111-118
·
IF 0.84
Congming Li,Zhou Yanli,Ye Baoxian,Xu Maotian
Abstract A novel, economic, and highly sensitive electrochemical sensor for the determination of trans -resveratrol ( RES ) was fabricated by electropolymerization of poly(L-lysine) films onto glassy carbon electrode ( GCE ) surface. The electrochemical behavior of RES in Britton–Robinson buffer solution (pH 2.0) on the poly(L-lysine)/GCE was investigated by cyclic voltammetry and linear sweep voltammetry for the first time. Results showed that the detection range of the proposed sensor to RES was from 0.20 to 12.0 μM with the minimum detectable concentration of 0.06 μM. The reported electrochemical sensor also exhibited high selectivity, good reproducibility, and long-term stability for RES detection. In addition, the proposed method can be employed for the determination of RES in red wines with satisfactory results. The proposed electrochemical strategy presents valuable information related to evaluation of RES levels in real samples.
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JOURNAL OF ETHNOPHARMACOLOGY
·
2020
·
IF 3.69
Shuangshuang Liu,Yue Li,Fan Yi,Qing Liu,Naihong Chen,Xiaoli He,Chunnian He,Peigen Xiao
Ethnopharmacological relevance Paeonia suffruticosa Andr. has been widely used in traditional Chinese medicine as an anti-tumour, anti-oxidant, anti-inflammatory and neuroprotective agent . Resveratrol oligomers are the main components of the seed coat extracts of Paeonia suffruticosa (PSCE) and have DPPH free radical scavenging and β-secretase inhibitory activity. However, studies of its effect on ameliorating cognitive deficits are limited, and analyses of the underlying mechanisms are insufficient. Aim of study This study aimed to investigate the cholinesterase inhibitory activities of resveratrol oligomers from P. suffruticosa in vitro and their effects on diminishing the oxygen-glucose deprivation/reoxygenation (OGD/R) -induced cytotoxicity in PC12 cells and scopolamine-induced cognitive deficits in mice. Moreover, the underlying mechanisms were further explored. Materials and methods In vitro , the inhibitory effects of PSCE and its 10 stilbenes on acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) were evaluated using the Ellman's assay, and its protective effects on normal and OGD/R-injured PC12 cells were evaluated using the MTT assay. For the in vivo assay, C57BL/6 mice were orally administered with PSCE at doses of 150 and 600 mg/kg for 28 days, and injected with scopolamine (1.5 mg/kg) to induce cognitive deficits. The memory behaviours were evaluated using the novel object recognition, Morris water maze and inhibitory avoidance test. Levels of various biochemical markers were also examined, including AChE, choline acetyltransferase (ChAT), acetylcholine (ACh), superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) in the mouse brain and interleukin-1β (IL-1β), interleukin-6 (IL-6), tumour necrosis factor-α (TNF-α), interleukin-4 (IL-4) in serum. Results PSCE and its 10 stilbenes display good inhibition of AChE and BuChE activities and significantly increase the viability of normal and OGD/R-injured PC12 cells. PSCE improves the cognitive performance of scopolamine-treated mice in behavioural tests. Meanwhile, PSCE increases AChE, ChAT, SOD, and CAT activities and ACh, GSH, IL-4 levels, and decreases IL-1β, IL-6, TNF-α levels in the model animals. Conclusions Resveratrol oligomers from P. suffruticosa show neuroprotective effect in vitro and in vivo by regulating cholinergic , antioxidant and anti-inflammatory pathways, may have promising application in the treatment of Alzheimer’s disease.
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IUBMB LIFE
·
2017
·Vol. 69
·期 7
·500-509
·
IF 3.141
Yanbin Su,Lei Chen,Yanwen Su,Zhengqiang Li,Chuang Zhang,Tongxing Mu
The purpose of this study is to probe the spectroscopic evidences of toxic trans-crotonaldehyde (TCA) trapped and transformed by resveratrol (Res) to prevent the damage of mitochondrial DNA. In aldehyde dehydrogenase (ALDH) at the different pH or mitochondria, the spectroscopic characteristics of TCA trapped and transformed by Res were observed by means of both UV-vis and Raman spectra. When Res interacted with TCA, TCA peak at 316 nm immediately disappeared while Res main peak at 305 nm and shoulder peak at 320 nm were dramatically changed, Raman peaks of TCA at 1,688 cm −1 assigned to CHO and 1,641 cm −1 affiliated to CC were strikingly shifted, Raman peaks of Res itself were significantly displaced or disappeared, especially in mitochondria and ALDH at different pH. The active groups of Res were the OH at C 5 and C 10 . The results of theoretical calculations are in agreement on the whole with the experimental data. The Res plays undoubtedly an important role via the structural change in TCA. The toxic CHO of TCA was effectively trapped and transformed by Res by means of itself OH at C 5 and C 10 . The mitochondrial alkaline microenvironment and ALDH promoted the elimination of toxic TCA. © 2017 IUBMB Life, 69(7):500–509, 2017
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International Journal of Nanomedicine
·
2025
·
IF 6.5
Chenchen Geng,Liuyang Yan,Yunhao Li,Houcong Li,Yuxin Ji,Yuhan Xiao,Zhifa Wang,Xiaoqi Chen,Changjie Chen,Qingling Yang,Baoding Tang,Wenrui Wang
Background Breast cancer is one of the most common cancers among women. Tumor cell proliferation is highly dependent on aerobic glycolysis, so regulating aerobic glycolysis in breast cancer cells is a promising therapeutic strategy. Resveratrol (Res), as a potential new anti-breast cancer drug, has been shown to regulate the glycolysis of cancer cells and inhibit the metastasis and recurrence of breast cancer. The nano drug delivery system can regulate the aerobic glycolysis metabolism by targeting the signaling factors and reaction products of the tumor aerobic glycolysis process to enhance the anti-tumor effect.Methods A new albumin-modified layered double hydroxide resveratrol dosage form (BSA@LDHs-Res) was synthesized by hydrothermal co-precipitation. Characterization was carried out to determine the successful synthesis of the nanocarrier system. The bioactivity, glycolytic activity and biocompatibility were examined by in vitro cellular assays; in vivo experiments were performed to further evaluate the anti-tumor effects of the BSA@LDHs-Res dosage form for breast cancer.Results In this study, we obtained for the first time a bovine serum albumin-modified BSA@LDHs-Res loaded dosage form, which was able to enter breast cancer cells SKBR3 and MDA-MB-231 via endocytosis and successfully escaped from lysosomal capture. BSA@LDHs-Res inhibited the proliferation, migration, and invasion of two types of breast cancer cells, induced apoptosis, and promoted the reduction of mitochondrial membrane potential and ROS. BSA@LDHs-Res inhibited the expression and viability of the key enzymes of glycolysis, hexokinase 2 (HK2), pyruvate kinase (PK), and lactate dehydrogenase, resulting in decreased glucose consumption, decreased lactate accumulation, and decreased intracellular ATP levels. BSA@LDHs-Res was examined in the mouse model with good anti-tumor effects.Conclusion BSA@LDHs-Res is an efficient nanoreagent for the treatment of breast cancer. The albumin-modified resveratrol layered double hydroxide delivery system developed in this study will provide some theoretical references for further research and clinical application of tumor aerobic glycolysis.
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ACS Sustainable Chemistry & Engineering
·
2024
·
IF 7.1
Xingwei Xun,Dongping Chen,Xi-Cun Wang,Xiaofeng Wu,Zheng-Jun Quan
Organic polysulfides have garnered significant attention recently as functional materials due to their abundant S–S bonds and strong chemical bonding structures, with potential applications in heavy metal adsorption, antimicrobials, and lithium–sulfur batteries. However, the environmental concerns associated with petroleum-based polymers and the flammability of sulfur pose challenges for their applications. This study describes the successful preparation of an inverse vulcanization sulfur-rich polymer using biobased resveratrol allyl ether as a copolymerization monomer. The polymer exhibits an ultrahigh glass transition temperature (Tg = 148 °C) and thermal stability, excellent flame retardancy, highly efficient silver absorption (removal rate >99.9%), outstanding silver-regenerative antimicrobial properties (complete inhibition of the growth and propagation of Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli)), as well as excellent specific discharge capacity (1198 mAh g–1), cycling performance, and rate capability. In situ battery infrared and DFT studies indicate that during the electrochemical redox process, the structure of the polymer undergoes a reorganization. The high active sulfur loading electrodes (∼5 mg cm–2) are also prepared to exhibit a certain practical potential with good discharge capacity and capacity retention.
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ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Xiaobang Liu,Jiaqun Du,Junpeng Sun,Han Wang,Jinyu An,Yingqiao Li,Yu Hu,Shuai Cheng,Huicong Feng,He Tian,Xifan Mei,Yang Qiu,Chao Wu
Spinal cord injury (SCI) is a serious neurological disease that can result in paralysis. After SCI occurs, the blood–spinal cord barrier (BSCB) is disrupted, and permeability is transiently elevated. However, the permeability of the BSCB returns to normal over time, which prevents many drugs from being used in subsequent treatments. In this study, we designed a borneol-functionalized macrophage membrane encapsulating mesoporous selenium nanoparticles loaded with resveratrol (MSe-Res-BMMs) for SCI treatment. In vivo animal experiments and in vitro cell experiments demonstrated that MSe-Res-BMMs were able to protect neurons from ferroptosis by reducing ROS levels and increasing glutathione peroxidase-4 (GPx-4) activity. In addition, this treatment also reduced ROS-induced inflammation and apoptosis by decreasing the expression of inflammatory factor IL-1β and apoptotic factor Cleaved Caspase-3 at the site of injury. Therefore, MSe-Res-BMMs are expected to provide new therapeutic options for SCI treatment.
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危险品化学品经营许可证（带存储）