Cell Division
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2026
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IF 4.9
Zhang Yichi,Li Nijina,Zhang Hao,Hu Dehua
Objective This study aimed to investigate the biological function and the molecular mechanism of ENDOD1 in colorectal cancer (CRC) progression. Methods CRC-related differentially expressed genes (DEGs) were screened by analyzing GEO datasets (GSE41657, GSE8671, GSE41328, and GSE75548). The differential expression of ENDOD1 within human CRC cell lines (HCT116, HT-29, SW620, and SW480) versus human normal colonic epithelial cells (NCM460) was validated using RT-qPCR and Western blot. ENDOD1 overexpression vector and siRNA were constructed and transfected into HT29 cells. CCK-8, flow cytometry, scratch test, and Transwell assays were performed to assess HT29 cell viability, apoptosis, and migratory and invasive abilities. The regulatory role of ENDOD1 in tumor growth in vivo was investigated using a xenograft mouse model. Moreover, a Western blot was performed to determine Wnt/β-catenin signaling activation. Results ENDOD1 was down-regulated in CRC tissues of GSE41657, GSE8671, GSE41328, and GSE75548 datasets, as well as in CRC cell lines. ENDOD1 overexpression promoted HT29 cell apoptosis and suppressed cell viability, migration, and invasion; ENDOD1 downregulation suppressed HT29 cell apoptosis and enhanced cell viability, migration, and invasion. In vivo, ENDOD1 overexpression inhibited tumor growth, suppressed Ki67 expression, and promoted apoptosis in transplanted tumors in mice. Moreover, EMDOD1 overexpression was blocked, while ENDOD1 inhibition promoted the activation of the Wnt/β-catenin pathway. Pharmacological activation of the Wnt/β‑catenin pathway partially attenuated ENDOD1-mediated suppression in CRC cells. Conclusion ENDOD1 expression level is decreased in CRC, and overexpression of ENDOD1 induces apoptosis, represses cell proliferation, migratory and invasive ability, and inhibits the Wnt/β-catenin signaling activation within CRC cells.
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高影响因子
Systems Microbiology and Biomanufacturing
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2026
·Vol. 6
·期 3
·80
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IF 3.5
Sun Ziqi,Hu Haonan,Wu Kexin,Zhang Yuting,Wang Xiaoli,Zhou Nandi
This study developed a highly selective and sensitive surface-enhanced Raman scattering (SERS) aptasensor for detecting enrofloxacin (ENR), a persistent fluoroquinolone antibiotic posing environmental concerns. This SERS aptasensor is constructed using flexible carbon cloth (CC) as the substrate, which is oxidized and then modified with titanium dioxide (TiO 2 ), followed by loading with gold nanoparticles (AuNPs) and functionalization with ENR-specific aptamer (Apt). The flexible nature of CC enables the substrate to adapt to irregular surfaces, providing structural support for potential portable on-site detection applications. It leverages a synergistic signal enhancement effect from localized surface plasmon resonance (LSPR) of AuNPs and the likely charge-transfer contribution of TiO 2 . A partial least squares (PLS) regression model correlated full spectral profiles with ENR concentrations, enabling accurate quantification while effectively mitigating spectral complexity and matrix interference. Under optimized conditions, the aptasensor exhibits a wide linear detection range (1–10 4 nmol/L) with a low detection limit of 0.37 nmol/L. It demonstrates good selectivity against interferents, high reproducibility, with relative standard deviation (RSD) at 1.87%, and excellent stability over 14 days (RSD below 2.83%), and accurate spiked recoveries in real water samples (96.94%–109.47%). These results show the great potential of the proposed SERS aptasensor for reliable and practical monitoring of ENR in environmental applications.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
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2026
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IF 4.7
He Yujiao,Xie Rongxiang
Background Stroke is a class of acute cerebrovascular diseases, among which ischaemic stroke is characterized by high morbidity and high recurrence rate. Due to multiple pathological mechanisms, it is very easy to cause cerebral ischaemia-reperfusion injury (RI). To quickly and effectively regulate the complex and variable cerebral immune microenvironment (IM) and reduce RI, the study aims to use a versatile immunosuppressive nanosystem (VIN) to efficiently remodel the overactivated brain IM and alleviate RI, thereby enhancing the effects of ischemic stroke (IS). Methods: The study first induced high expression of CXCR4 receptor in rat bone marrow mesenchymal stem cell membranes using Fe 3 O 4 nanoparticles, then extracted the cell membranes by hypotonic lysis and repeated freeze-thawing and reconstituted using ultrasound to form engineered bionic decoy vesicles. Next, polydopamine nanoparticles were prepared by oxidative self-polymerization, using Zn 2+ as a bridge to obtain A151-loaded polydopamine nanoparticles. Finally, it was reconstituted with engineered bionic decoy vesicles using ultrasonic cavitation to prepare a VIN integrated with engineered bionic decoys. Results: It was shown that Fe 3 O 4 nanoparticles (Fe 3 O 4 NPs) could significantly increase the expression level of CXCR4 receptor. In rats treated by the VIN group, the body weight had increased to near the normal level by day 7, and the infarct area was reduced by 89.4%. The results showed that VIN was able to effectively reduce the volume of cerebral ischemic infarction in rats in long-term treatment, and had a good therapeutic effect on IS. Conclusion: The study achieved internal and external synergistic immunosuppressive effects to effectively and safely regulate the excessive IM after reperfusion, which provides a new idea to alleviate ischaemia-RI. The alternative text for this image may have been generated using AI.
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JOURNAL OF MATERIALS SCIENCE
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2026
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IF 4.4
Cao Xingwang,Zeng Rui,Yao Mingyue,Li Bo,Miao Wenjun,Feng Hao,Li Shan,Pu Qingrong,Yuan Yuan,Dong Li,Huang Rui
Myocardial fibrosis is a pivotal pathological process driving the progression of cardiovascular disease, with myofibroblasts originating from endothelial-to-mesenchymal transition (EndMT) playing a central role in fibrotic remodeling. However, current therapeutic strategies remain suboptimal, exhibiting significant limitations in clinical application and failing to adequatelyaddress the treatment demands of myocardial fibrosis. In this study, we developed an innovative injectable hydrogel, Scu@PUE, endowed with EndMT-modulating properties. This hydrogel was precisely engineered through the spontaneous supramolecular assembly of Puerarin (PUE) and Scutellarin (Scu) under strictly regulated thermodynamic conditions, ensuring structural stability and biological functionality. To comprehensively assess its therapeutic potential, we conducted systematic in vivo and in vitro evaluations to elucidate the mechanistic role of Scu@PUE in suppressing EndMT and ameliorating myocardial fibrosis. Experimental findings demonstrated that Scu@PUE exhibits an optimized spatial architecture, while molecular dynamics simulations further revealed a stable and efficient intermolecular interaction between PUE and Scu. Pharmacodynamic analyses confirmed the significant therapeutic efficacy of Scu@PUE in the treatment of myocardial fibrosis, particularly in an isoproterenol (ISO)-induced murine model of cardiac dysfunction. Scu@PUE administration effectively enhanced cardiac ejection fraction improved ventricular wall thickness, and markedly alleviated fibrosis-associated pathological manifestations. Furthermore, mechanistic investigations indicated that Scu@PUE treatment significantly attenuated EndMT during myocardial fibrosis progression. This regulatory effect was found to be mediated, at least in part, via suppression of the IFN-γ-STAT1 signaling axis, thereby mitigating EndMT-driven pathological remodeling. In conclusion, this study introduces a novel EndMT-targeted therapeutic strategy based on the precisely designed injectable hydrogel Scu@PUE, offering a promising approach for the treatment of myocardial fibrosis with potential translational implications in clinical applications.
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MICROCHIMICA ACTA
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2026
·Vol. 193
·期 5
·341
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IF 5.4
Gao Songbing,Li Qianfeng,Zhai Haifa,Wang Gongke
Biological thiols (biothiols), such as cysteine (Cys), glutathione (GSH), and homocysteine (Hcy) are essential for maintaining life activities, and their abnormal concentrations are closely associated with various diseases. We developed a dual-mode sensor using synergistically passivated CsPbBr₃ quantum dots (QDs). By introducing the ligand sodium dodecyl sulfate (SDS) into CsPbBr₃ QDs and combining the specific recognition ability of 5,5’-dithiobis-(2-nitrobenzoic acid) (DTNB) toward thiol groups (–SH), a dual-mode detection system was constructed. In the presence of biological thiols, the –SH group reacts with DTNB to generate 2-nitro-5-thiobenzoic acid (TNB). A discernible color change of the solution from light green to yellow, along with fluorescence quenching of the CsPbBr₃@SDS QDs, was observed. This is attributed to the inner-filter effect (IFE), which is initiated by the overlap of the TNB absorption spectrum with the excitation spectrum of the QDs. This enables the dual-mode detection of target analytes via fluorescence and colorimetric signals. The sensor demonstrates high sensitivity toward Cys, GSH, and Hcy, with detection limits as low as 2.63 µM, 3.09 µM, and 2.15 µM, respectively, along with a wide linear range. The sensor also exhibits excellent selectivity and anti-interference capabilities. This work provides an effective strategy for biothiol detection, showing promising application prospects in biomedical diagnosis and environmental monitoring.
Section
Graphical abstract
The alternative text for this image may have been generated using AI.
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高影响因子
JOURNAL OF MATERIALS SCIENCE
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2026
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IF 4.4
Gu Zhanbin,Lv Shuwei,Sun Xirui,Mao Chunyu,Zhai Ying,Zhao Meng,Zhang Lipeng
The problem of surface icing affects the stable operation of equipment and facilities. In order to avoid icing problems, many measures have been tried. However, how to achieve efficient and lasting anti-icing and de-icing is still an urgent problem to be solved. Here, nanomaterials with photothermal response and modified microstructure particles were added to the polymer, and the photothermal response superhydrophobic (PRSH) coating with photothermal response characteristics was obtained by spraying. The droplet contact angle on the aluminum surface with PRSH coating can reach 161.2°, and the rolling angle is only 3.3°, showing excellent superhydrophobicity. Under near-infrared irradiation, the surface temperature of aluminum with PRSH coating can reach more than 130 °C. Even at a low temperature of − 20 °C, the surface temperature can still reach more than 80 °C. The surface can achieve passive anti-icing and active de-icing, the complete freezing time of the droplets can be delayed by more than 230%, and the frozen droplets can completely melt within 40 s. Mechanical friction, chemical corrosion and other tests show that the PRSH coating has good durability and wide applicability. Graphical abstract
The alternative text for this image may have been generated using AI.
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JOURNAL OF MATERIALS SCIENCE
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2026
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IF 4.4
Li Yifan,Li Zhen,Zhang Yuyuan,Wang Menghe,Ye Shuwen,Wu Bailu,Lv Peijie,Pang Pengfei,Li Xin,Zhan Pengchao
Tirapazamine (TPZ) is a bioreductive agent with selective antitumor activity under hypoxic conditions; however, its systemic toxicity and delivery efficiency limit its clinical application. This study aimed to develop a TPZ delivery system based on nanobubbles (NBs), combining ultrasound-targeted microbubble destruction (UTMD) and transcatheter arterial chemoembolization (TACE) for treating hepatocellular carcinoma (HCC) to enhance therapeutic efficacy and reduce adverse effects. TPZ-NBs were fabricated using a high-shear dispersion method and characterized for their physicochemical properties, encapsulation efficiency, and drug release profiles. In vitro studies evaluated the effects of TPZ-NBs on hepatoma cell proliferation and migration under hypoxic conditions. Using a rabbit VX2 liver cancer model, we compared the control group, TACE group, TPZ-NBs + TACE group, and TPZ-NBs + UTMD + TACE group in terms of tumor necrosis, drug delivery efficiency, and safety profiles. TPZ-NBs demonstrated favorable encapsulation efficiency (35.81%) and sustained-release characteristics (58% cumulative release over 48 h). In vitro experiments showed that TPZ-NBs significantly inhibited hepatoma cell proliferation and migration under hypoxic conditions. In animal studies, the TPZ-NBs + UTMD + TACE group significantly enhanced intratumoral drug delivery efficiency, induced tumor cell apoptosis, reduced microvessel density (MVD), and decreased tumor recurrence. Compared to other groups, this combination therapy exhibited higher tumor necrosis rates and deeper drug penetration while maintaining favorable safety profiles. The combination of TPZ-NBs with UTMD and TACE represents an efficient and safe therapeutic strategy that overcomes the limitations of conventional TACE, offering new possibilities for hepatocellular carcinoma treatment.
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PHARMACEUTICAL RESEARCH
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2026
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IF 4.1
Sun Xiaoyan,Qin Yaxin,Ben Zhiqing,Zhang Zhicheng,Bao Xiaoyan,Yang Yufan,Shui Yongjie,Chen Haiyan,Wu Dang,Wei Qichun,Han Min
Objective Boron neutron capture therapy (BNCT) is limited by inadequate boron delivery to tumor cells and the inability to visualize boron agents for personalized treatment. This study introduces DOTA-BPA-Gd, a dual-function boron agent integrating BNCT efficacy with drug tracing. Methods DOTA-BPA-Gd was designed with four phenylboronic acid groups to target sialic acid residues on cancer cell surfaces. This strategy aims to enhance tumor boron accumulation while avoiding LAT1-mediated exocytosis and transporter saturation associated with BPA-F. With a molecular weight of ~ 1200 Da, DOTA-BPA-Gd allows efficient spatial binding to sialic acid, improving targeting specificity. Cellular uptake, cytotoxicity, and boron distribution were evaluated, followed by in vivo BNCT experiments with MRI monitoring. Results DOTA-BPA-Gd effectively delivered boron to cancer cells without detectable toxicity. Under neutron irradiation, the DOTA-BPA-Gd + N group showed significant tumor inhibition, with tumor volumes reduced to about one-third of those in the BPA-F + N group. Furthermore, gadolinium enabled real-time MRI-based tracking of boron biodistribution, confirming the agent’s dual functionality. Conclusions DOTA-BPA-Gd offers a promising strategy for BNCT by combining targeted boron delivery with non-invasive imaging. This integrated approach improves treatment precision, enhances therapeutic efficacy, and may help overcome key clinical challenges in BNCT. Graphical
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Journal of Biological Engineering
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2026
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IF 6.3
Zhu Longchuan,Yan Hongye,Li Hongru,Pan Su,Yang Hanwen,Zhu Zhe,Qi Zhiping
Nerve regeneration and axonal growth can be significantly enhanced by electrical stimulation (ES) using conductive polymers. But in addition to ES, nerve cell function and how they react to substrate conductivity are also essential factors in nerve repair. As a result, ES, in conjunction with appropriate materials (such as tissue scaffolds), has been used to treat nerve damage and has demonstrated significant promise for peripheral nerve regeneration. In this work, electrospinning was used to create conductive composite scaffolds made of polyglycolic acid@polycaprolactone/aniline pentamer (PGCL/AP). We later verified that PC12 cells cultured on these scaffolds exhibited improved migration, proliferation, and vitality upon electrical stimulation. Furthermore, In the established 10-mm peripheral nerve injury model, the PAP + ES group achieved outcomes comparable to the autograft group in the assessment of functional and morphological recovery, encompassing SFI, myelination rate, and gastrocnemius muscle evaluation. Graphical
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Journal of Food Measurement and Characterization
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2026
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IF 3.6
Shen Yemeng,Fei Siyuan,Bai Yihua,El-Aty A. M. Abd,Tan Mingqian
Cognitive impairment is considered as a neurological deficit, which affects millions of people worldwide. Docosahexaenoic acid (DHA), an essential part of brain cell membranes, is crucial for supporting neural communication and preventing cognitive decline. To address this concern, emulsion fillers comprising salmon myofibrillar protein (SMP), Konjac glucomannan (KGM), and octenyl succinic anhydride (OSA)-starch were used. An DHA-loaded emulsion gel stabilized by OSA-satrch through the modulation of the oil volume fraction was developed to strengthen the structure of SMP gels. The stabilization by the SMP-KGM gel and OSA-starch SMP–KGM (OSA-SMPG) promoted the formation of a compact three-dimensional network structure in the emulsion gel, which resulted in an increased storage modulus, enhanced viscoelastic properties, and good thixotropic behavior. DHA-loaded OSA-SMPG emulsion gel alleviated organ atrophy in mice and reduced escape latency by 48% while improving learning and memory abilities. The total cholesterol level in mouse serum decreased by 32%. In addition, OSA-SMPG alleviated the oxidative stress induced by D-galactose in mice, increased the body’s antioxidant defenses, and increased superoxide dismutase levels in brain tissue by 43%. These findings suggest that promising prospects and potential for nutritional intervention in individuals with cognitive impairment are offered by OSA-SMPG.
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Science China-Chemistry
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2026
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IF 9.8
Liu Zhaozhuang,Wang Mingxi,Wang Li,Liu Jiaqi,Li Jinping,Yang Jiangfeng
The efficient separation and enrichment of methane (CH 4 ) from low-concentration coalbed methane (CBM) are of great significance for enhancing CBM utilization efficiency and promoting the development of low-carbon clean energy. To address this practical need, this study synthesized a tailored chlorinated metal-organic framework (MOF), PCP-IPA-Cl, and systematically evaluated its CH 4 /N 2 separation performance through a combination of static adsorption measurements, density functional theory (DFT) calculations, dynamic breakthrough experiments, and multiple adsorption-desorption cycling tests. Endowed with its optimized pore structure and chlorinated channel environment, PCP-IPA-Cl exhibits excellent CH 4 /N 2 separation performance and regeneration stability: at 298 K and 1 bar, it achieves a high CH 4 adsorption capacity of 21.27 cm 3 /g with a low N 2 uptake of only 5.29 cm 3 /g, and compared with its unchlorinated analog PCP-IPA, the CH 4 /N 2 selectivity is markedly improved from 5.4 to 9.0. DFT calculations reveal that the introduction of–Cl groups creates additional preferential adsorption sites for CH 4 molecules, strengthening host-guest interactions and thus enhancing separation efficiency. Furthermore, breakthrough experiments confirm its excellent dynamic separation ability, and cyclic tests demonstrate that PCP-IPA-Cl can be fully regenerated via helium purging within just 5 min. These findings collectively demonstrate that PCP-IPA-Cl is a promising and regenerable adsorbent for the efficient enrichment and utilization of low-concentration CBM.
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Discover Oncology
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2026
·Vol. 17
·期 1
·720
·
IF 2.8
Duan He,Li Ning,Song Dingming,Li Yongzhuo,Liang Xin,Ding Yongxue,Tong Ming
Introduction Clear cell renal cell carcinoma (ccRCC) is a prevalent malignancy of the kidney, known for its aggressive growth and tendency to metastasize. Cellular senescence (CS), regarded as a characteristic feature of the natural aging process, is strongly linked to the initiation and advancement of several diseases. This research seeks to develop an integrated prognostic model utilizing senescence markers in ccRCC, with the goal of evaluating clinical outcomes and clarifying the tumor’s immune microenvironment. Methods The transcriptome profiles from ccRCC patients were acquired from TCGA database, and the senescence-related genetic model (SRGM) was developed through Cox proportional hazards analysis integrated with multiple machine learning approaches. In addition, immune microenvironment and drug sensitivity analysis were performed. EIF4EBP1 expression and its role in epithelial cell senescence were analyzed using single-cell RNA sequencing, spatial transcriptomics, and trajectory analysis. Finally, cellular experiments were conducted to confirm the role of silencing EIF4EBP1 in the biological behavior of ccRCC cells. Results Through a comprehensive framework incorporating 117 machine learning algorithm combinations, we established an SRGM consisting of 9 genes. The optimal model was constructed using CoxBoost + StepCox[forward] and achieved a concordance index (C-index) of 0.743, demonstrating robust prognostic predictive capacity across independent datasets. EIF4EBP1 is a central gene in the model. Silencing EIF4EBP1 reduced cell migration, proliferation, and invasion, while inducing senescence-associated phenotypes and apoptosis in ccRCC cells. Additionally, it significantly enhanced the responsiveness of ccRCC cells to sunitinib treatment. Conclusions In summary, we developed a novel SRGM that effectively stratifies prognosis in ccRCC. Furthermore, EIF4EBP1 has been validated at the cellular level as a promising therapeutic target, providing innovative insights into the personalized treatment of ccRCC.
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