CHROMATOGRAPHIA
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2025
·
IF 1.3
Lou Doudou,Zhu Yuan,Fan Jingke,Fan Lin,Zhu Qingfu,Lu Yihong,Zhao Shuqiang
N-glycosylation heterogeneity has a significant impact on the clinical safety, efficacy, pharmacokinetics (PK), and pharmacodynamics of monoclonal antibody (mAb) drugs, thus becoming a critical quality attribute (CQA) of great concern. In the registration quality standards for bevacizumab produced by different manufacturers, either lacking supervision of N-glycosylation or the detection methods and criteria vary greatly among manufacturers, leading to compromised comparability of results. Besides, the existing quality control system pays insufficient attention to the correlation between glyco-relevant physicochemical properties and glycosylated modification. In this study, a standardized hydrophilic interaction liquid chromatography-fluorescence detection (HILIC-FLD) N-glycan profiling method was developed, which had been proven to be more time-saving and easier to operate and was used for the parallel analysis of N-glycan profiles in bevacizumab products provided by 8 different manufacturers. We also paid attention to the glycosylation-related CQAs including charge heterogeneity, isoelectric point, non-glycosylated heavy chain (NGHC) and aggregates. Results revealed obvious difference in N-glycosylation, NGHC and aggregates among manufacturers. Among all the mAbs, the abundance of major glycoforms showed similar order yet the percentage of the same glycoform varies greatly. In summary, the standardized method could offer a reliable, efficient and practical approach for the parallel analysis and comparison of glycosylation and related CQA heterogeneity among manufacturers, providing a reference for more effective quality control of mAb drugs.
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Nature Communications
·
2022
·Vol. 13
·期 1
·1-12
·
IF 17.694
Yang Nailin,Gong Fei,Liu Bo,Hao Yu,Chao Yu,Lei Huali,Yang Xiaoyuan,Gong Yuehan,Wang Xianwen,Liu Zhuang,Cheng Liang
Hydrogen can be used as an anti-cancer treatment. However, the continuous generation of H 2 molecules within the tumor is challenging. Magnesium (Mg) and its alloys have been extensively used in the clinic as implantable metals. Here we develop, by decorating platinum on the surface of Mg rods, a Mg-based galvanic cell (MgG), which allows the continuous generation of H 2 in an aqueous environment due to galvanic-cell-accelerated water etching of Mg. By implanting MgG rods into a tumor, H 2 molecules can be generated within the tumor, which induces mitochondrial dysfunction and intracellular redox homeostasis destruction. Meanwhile, the Mg(OH) 2 residue can neutralize the acidic tumor microenvironment (TME). Such MgG rods with the micro-galvanic cell structure enable hydrogen therapy to inhibit the growth of tumors, including murine tumor models, patient-derived xenografts (PDX), as well as VX 2 tumors in rabbits. Our research suggests that the galvanic cells for hydrogen therapy based on implantable metals may be a safe and effective cancer treatment.
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高影响因子
Scientific Reports
·
2018
·Vol. 8
·期 1
·1-9
·
IF 4.122
Zhang Meng,Zhang Fan,Wang Yue,Zhu Lijie,Hu Yufeng,Lou Zhidong,Hou Yanbing,Teng Feng
Photodetectors based on three dimensional organic–inorganic lead halide perovskites have recently received significant attention. As a new type of light-harvesting materials, formamidinium lead iodide (FAPbI 3 ) is known to possess excellent optoelectronic properties even exceeding those of methylammonium lead iodide (MAPbI 3 ). To date, only a few photoconductor-type photodetectors based on FAPbI 3 single crystals and polycrystalline thin films in a lateral structure have been reported. Here, we demonstrate low-voltage, high-overall-performance photodiode-type photodetectors in a sandwiched geometry based on polycrystalline α-FAPbI 3 thin films synthesized by a one-step solution processing method and post-annealing treatment. The photodetectors exhibit a broadband response from the near-ultraviolet to the near-infrared (330–800 nm), achieving a high on/off current ratio of 8.6 × 10 4 and fast response times of 7.2/19.5 μs. The devices yield a photoresponsivity of 0.95 AW −1 and a high specific detectivity of 2.8 × 10 12 Jones with an external quantum efficiency (EQE) approaching 182% at −1.0 V under 650 nm illumination. The photodiode-type photodetectors based on polycrystalline α-FAPbI 3 thin films with superior performance consequently show great promise for future optoelectronic device applications.
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JOM
·
2024
·
IF 2.1
Saleem Muhammad Farooq,Ashraf Ghulam Abbas,Javid Muhammad,Haleem Yasir A.,Haidry Azhar Ali,Farooq Sajid,Lei Ma,Bilal Muhammad,Habib Muhammad,Akhtar Muhammad Saeed,Khan Rashid,Wang Tianwu
Understanding the dielectric properties of materials in the THz frequency range is crucial for the advancement of THz devices. Despite their significance, the dielectric characteristics of zirconium carbide (ZrC) within this frequency range remain underexplored, in particular the impact of surface carbon and oxide layers typically found on carbide particles. While traditional studies mainly focus on particle size, this study emphasizes the pivotal role of particle morphology in determining dielectric properties. Milling was employed to increase the surface exposure of ZrC particles, which were initially covered with layers of carbon and oxides. The dielectric parameters, derived from experimental THz time-domain spectroscopy (THz-TDS) data, indicated that increased milling led to improved energy storage capabilities. This enhancement is attributed to increased electronic polarizability and charge disorder-induced ionic polarization due to the increased surface exposure. Furthermore, the dielectric properties displayed reduced dispersion after further milling attributed to the uniform particle sizes and shapes achieved through this process. Our findings underscore the critical influence of surface carbon and oxide layers on the dielectric properties of ZrC and similar carbide particles—an aspect often overlooked in studies that focus solely on particle size and shape, ignoring the distinct dielectric contributions of each surface layer.
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 5
·1-12
·
IF 5.3
Zhai Tong,Shen Yu-Di,Yu Miao,Liu Jing-Min,Wang Shuo
Robust detection techniques combined with functional micro-/nano-entities can be an ideal solution for food-safety risk treatment related to aflatoxin B1 (AFB1). In this work, a porous covalent architecture-involved luminescent smart hydrogel with dynamic cross-linking and target-responsive framework was prepared for specific determination of AFB1 with remarkable sensitivity. Different from the existing hydrogel probe, the introduction of covalent organic frameworks (COFs) enhanced the hydrophobicity of hydrogel, enabling the selective capture of AFB1 with improved affinity while excluding large matrix molecules, thus creating an anti-interference detection environment. Additionally, the pH-responsive carbon dots (CDs) embedded in the smart hydrogel contributed to a dual signal amplification, induced by deconstruction of hydrogel to release the CDs and urease-catalyzed hydrolysis reaction to increase the pH, when the AFB1 presented. In view of this signal amplification and trace enrichment strategy, this smart hydrogel can achieve robust determination of AFB1 with a detection limit of 0.03 µM as well as good specificity. The method was validated using real-world samples, demonstrating excellent accuracy and precision compared to standard HPLC methods. This work not only advances the field of AFB1 detection but also provides a versatile and cost-effective platform that can be adapted for the detection of other toxins and biomolecules, addressing global food safety and environmental monitoring challenges. Graphical abstract
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 5
·1-13
·
IF 5.3
Li Ruidan,Tian Li,Song Yujia,Guo Yanjia,Ma Guangping,Han Pengfei,Jiang Hanyue,Wang Wenzhuo,Lu Juan
The self-supporting material Zn3Cu2O2 was synthesized through the methods of electrospinning combined with high-temperature calcination. A novel sensitive electrochemiluminescence (ECL) sensor based on Zn3Cu2O2 and CdTe@MWNTs was constructed by the multi-step amplification strategy. First, Zn3Cu2O2 and CdTe served as the ECL groups to construct the sensor, yielding a larger signal of the sensor in the presence of the co-reactant K2S2O8. Multi-walled carbon nanotubes (MWNTs) were introduced as a carrier to increase the signal value of the sensor further. The synergistic action of CdTe@MWNTs and Zn3Cu2O2 made the system obtain the maximum initial signal. With the addition of Allure Red (AR), the ECL signal decreased. The quenching phenomenon was not only due to the large organic molecule AR occupying the active sites of Zn3Cu2O2 and CdTe, but also, more importantly, because the sensor existed as an ECL-RET mechanism between CdTe and AR. The response mechanism and experimental conditions of the system were also investigated. Under optimal conditions, the sensor showed a linear relationship between the ECL signal change and the logarithm of AR concentration in the range 1.0 × 10−14 to 1.0 × 10−6 mol L−1, the linear equation was ∆I = 15,295.54 + 956.42 log CAR, and the correlation coefficient was 0.9928. The lowest detection limit of (S/N = 3) was 1.67 × 10−15 mol L−1. Satisfactory results were obtained for the analysis of beverages.
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JOURNAL OF POLYMER RESEARCH
·
2025
·Vol. 32
·期 4
·1-11
·
IF 2.8
Liang Miaoqing,Guan Fanglan,Zhang Mei,Nie Jinmei,Bao Lihong
Self-healing materials have gained significant attention due to their ability to autonomously repair damage, extending material lifespan. In this study, bio-based self-healing polyurethanes were synthesized using modified castor oil (MCO) as the soft segment and 5-(2-hydroxyethyl)- 6-methyl- 2-aminouracil (UPY) and 2,2'-diaminodiphenyldisulfide (DTDA) as dynamic components. The synergistic effect of quadruple hydrogen bonding and disulfide bonds was investigated by varying the n(UPY)/n(DTDA) ratio. Structural and mechanical properties were characterized using IR, DSC, TGA, XRD, and tensile testing. The results revealed that the castor oil-based polyurethanes exhibited transparency and amorphous structures, making them promising bio-based self-healing materials. When the DTDA content was 9.54% and the UPY content was 1.62%, the material achieved a self-healing efficiency of 96.38% under 80 °C for 12 h. Additionally, at a DTDA content of 6.65% and a UPY content of 2.43%, the tensile strength reached 17.76 MPa. Mechanistic analysis revealed that disulfide bonds played a dominant role in self-healing, while hydrogen bonds provided additional reinforcement. This work presents a novel bio-based polyurethane system with tunable mechanical and self-healing properties, contributing to the development of sustainable smart materials.
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EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2024
·
IF 3
Wen Jinli,Sun Yining,Yan Yiping,Yuan Pengqiang,He Yanli,Sun Bowei,Zhao Zihao,Lu Wenpeng
This study used Actinidia arguta ‘Kuilv’ as raw material to produce wine by two different methods: whole pulp and clear juice fermentation. The basic physicochemical properties, nutrients, antioxidant capacity, volatile flavor compounds, and sensory evaluation of Actinidia arguta wine were detected. The results indicated that clear juice fermentation increased the ethanol content and color quality, and decreased the residual sugar content. Whole pulp fermentation reduced the total acid content and the total amount of organic acid and increased the pH value, antioxidant active ingredients, antioxidant activity, and the volatile flavor substances of the wine. High-performance liquid chromatography (HPLC) analysis showed that citric acid and quinic acid were the main organic acids in the wine. Headspace gas chromatography–ion mobility spectrometry (HS-GC–IMS) analysis detected 55 volatile flavor compounds in the wine, including 26 esters, 13 alcohols, 10 aldehydes, 3 ketones, 1 acid, 1 terpene, and 1 pyrazine. Fourteen key volatile flavor substances were identified based on P -value < 0.05 and VIP value > 1: 2-pentanone, isoamyl alcohol, propionaldehyde, ethyl isobutyrate, 4-methyl-1-pentanol, butanal, butyl acetate, 2-heptanone, ethyl isovalerate, acetal M, 3-methyl-3-buten-1-ol M, ethyl butyrate, 1-penten-3-ol, octanal.
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JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
·
2025
·
IF 1.6
He Yan,Du Wenlong,Fu Xiaolei,Yuan Dingzhong,Na Bing,Zhang Shaoze,Liu Honglai
Developing new porous polymer to separate uranium in water is an urgent task. In our work, a novel hypercross-linked porous polymer bearing phosphonate ligand (HCP-PL) was constructed by a simple Friedel–Crafts alkylation strategy. The obtained polymer HCP-PL was showed an excellent uranium adsorption capacity and outstanding selectivity in aqueous solution. The adsorption isotherm model is consistent with Langmuir monolayer isotherm. It is worth mentioned that the maximum uranium adsorption capacity ( q max ) by HCP-PL reached up to 553.4 mg/g, which was much higher than most reported porous materials. The polymer HCP-PL exhibits a pronounced selective adsorption capacity for uranium in the existence of other impurity ions, including Cu 2+ , Co 2+ , Sn 2+ , La 3+ , Ce 3+ , and Pb 2+ . This excellent selectivity is attributed to the strong affinity between uranium and phosphonate ligand, as demonstrated by experimental results and by density functional theory calculations. Moreover, the porous polymer HCP-PL remained removal uranium percentage of 82% after 5 adsorption–desorption cycles. Therefore, the novel hypercross-linked porous materials bearing phosphonate ligand have good application prospects in the removal of uranium ions in water.
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JOURNAL OF FLUORESCENCE
·
2025
·
IF 3.1
Ksenofontov Alexander A.,Berezin Michail B.,Bocharov Pavel S.,Khodov Ilya A.,Miloshevskaya Olga V.,Antina Elena V.
The search for fluorophores with intense absorption and emission in the red region of the spectrum is an important task, as such compounds can be used in fluorescence imaging, providing high image resolution due to the deep penetration of low energy photons into tissues. In this paper, we present the results of the synthesis and photophysical characterization of a novel ms -benzimidazole-4,4′,5,5′-tetramethyldipyrromethene bis (difluoroborate) ( BOIMPY ) compound, which exhibits intensive absorption and emission in the long-wavelength region while maintaining high fluorescence quantum yields (up to 60%). Using DFT analysis, we investigated the geometry of BOIMPY in both ground and excited states and described the influence of the molecular structure on its practically relevant photophysical properties.
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Nature Communications
·
2022
·Vol. 13
·期 1
·1-13
·
IF 17.694
Luo Huilong,Chen Yanmei,Kuang Xiao,Wang Xinyue,Yang Fengmin,Cao Zhenping,Wang Lu,Lin Sisi,Wu Feng,Liu Jinyao
Methods capable of manipulating bacterial colonization are of great significance for modulating host-microbiota relationships. Here, we describe a strategy of in-situ chemical reaction-mediated covalent localization of bacteria. Through a simple one-step imidoester reaction, primary amino groups on bacterial surface can be converted to free thiols under cytocompatible conditions. Surface thiolation is applicable to modify diverse strains and the number of introduced thiols per bacterium can be easily tuned by varying feed ratios. These chemically reactive bacteria are able to spontaneously bond with mucous layer by catalyst-free thiol-disulfide exchange between mucin-associated disulfides and newly converted thiols on bacterial surface and show thiolation level-dependent attachment. Bacteria optimized with 9.3 × 10 7 thiols per cell achieve 170-fold higher attachment in mucin-enriched jejunum, a challenging location for gut microbiota to colonize. As a proof-of-concept application for microbiota transplantation, covalent bonding-assisted localization of an oral probiotic in the jejunum generates an improved remission of jejunal mucositis. Our findings demonstrate that transforming bacteria with a reactive surface provides an approach to chemically control bacterial localization, which is highly desirable for developing next-generation bacterial living bioagents.
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Advanced Fiber Materials
·
2024
·Vol. 6
·期 2
·444-457
·
IF 17.2
Xiao Yuanxiang,Jiang Jingjing,Cai Rui,Fu Jiajia,Xiang Shuangfei,Zhao Shujun,Fu Feiya,Diao Hongyan,Liu Xiangdong
Unpredictable pandemics are likely to pose a significant global threat in the future, and biologically protective textiles will play critical roles in controlling the spread of pathogens during outbreaks. Herein, we present a novel metal–organic framework (MOF) composed of repeating units of a Cu(II)/(L-Cys) 2 complex formed through coordination bonds between Cu(II) and L-Cys, while being interconnected by ionic bonds involving Cu(II) and the carboxylate group of L-Cys. After covalently embedding the MOF nanofibers onto cotton fiber surfaces, the resulting fabrics exhibit remarkable virucidal and antibacterial capabilities. Remarkably, even after 200 friction or 50 laundering cycles, the high antiviral ability to inactivate all phi- × 174 within 10 min was maintained, and the bacterial reduction rate against E. coli and S. aureus remained nearly at 100%. The remarkable virucidal effect of the L-Cys@Cu MOF structure is elucidated through a series of α-amylase denaturation simulation tests, providing the first experimental demonstration of the antiviral mechanism, whereby MOF nanofibers induce protein denaturation to inactivate viruses. Moreover, cytotoxicity assessments confirm that the fabrics adorned with MOF nanofibers are safe for human skin. These advantages are promising for the development of protective textiles, highlighting the great potential of nanoscience in combating pandemics. Graphical Abstract
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