Asian Journal of Pharmaceutical Sciences
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2019
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IF 4.016
Shuying Li,Ying Zhang,Abdur Rauf Khan,Shuwang He,Yingxin Wang,Jiangkang Xu,Guangxi Zhai
The bitterness of a drug is a major challenge for patient acceptability and compliance, especially for children. Due to the toxicity of medication, a human taste panel test has certain limitations. Atomoxetine hydrochloride (HCl), which is used for the treatment of attention deficit/hyperactivity disorder (ADHD), has an extremely bitter taste. The aim of this work is to quantitatively predict the bitterness of atomoxetine HCl by a biosensor system. Based on the mechanism of detection of the electronic tongue (E-tongue), the bitterness of atomoxetine HCl was evaluated, and it was found that its bitterness was similar to that of quinine HCl. The bitterness threshold of atomoxetine HCl was 8.61 µg/ml based on the Change of membrane Potential caused by Adsorption (CPA) value of the BT0 sensor. In this study, the taste-masking efficiency of 2-hydroxypropyl-β-cyclodextrin (HP-β-CyD) was assessed by Euclidean distances on a principle component analysis (PCA) map with the SA402B Taste Sensing System, and the host–guest interactions were investigated by differential scanning calorimetry (DSC), powder X-ray diffraction (XRD), nuclear magnetic resonance (NMR) spectroscopy and scanning electron microscopy (SEM). Biosensor evaluation and characterization of the inclusion complex indicated that atomoxetine HCl could actively react with 2-hydroxypropyl-β-cyclodextrin.
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ENERGY & FUELS
·
2019
·
IF 3.021
Songjie Hua,Yuqi Hu,Liang Zhao,Liyuan Cao,Xiaoqin Wang,Jinsen Gao,Chunming Xu
For the effective removal of thiophene, a novel surface molecularly imprinted polymer (SMIP/MA@PCNS) was fabricated using porous carbon nanospheres (PCNS-5) as the support matrix and maleic acid (MA) as the functional monomer. Benefiting from the special amphiphilic properties of β-cyclodextrin as the carbon source, PCNS-5 exhibited good dispersion, uniform nanoscale sphere, and high specific surface area, which favored the rapid adsorption rate. Based on the intermolecular hydrogen bonds existing between carboxylic acid functional groups and thiophene, adsorption performances of different unsaturated polycarboxylic acids were investigated systematically. The results revealed that the optimal functional monomer was MA instead of the conventional methacrylic acid. Accordingly, SMIP/MA@PCNS was successfully applied to the direct removal of thiophene in the oil, reaching remarkable sulfur adsorption capacity (22.6 mg S g–1) within 120 min. Moreover, SMIP/MA@PCNS could selectively remove thiophene (16.2 mg S g–1) in the presence of structural analogues (3-methylthiophene, benzene, and cyclopentane) and exhibited an excellent regenerability with nice structural ability. These results indicated that SMIP/MA@PCNS possessed great promise in the desulfurization field.
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Analytical Methods
·
2019
·Vol. 11
·期 42
·5398-5404
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IF 2.378
Shengting Zhang,Mingrui Li,Dongbo Liu,Kunhao Qin,Yingying Xiang,Xiuling Ji,Congwen Li,Yunlin Wei
The human purinergic P2Y1 receptor (P2Y1R) belongs to the family of G-protein coupled receptors (GPCRs). Two stable cell lines, hP2Y1R-tGFP/U2OS and hP2Y1R-β-AR2-tGFP/U2OS, were generated with steady expression of fluorescent tGFP tagged P2Y1R or tGFP tagged β-arrestin2. The High Content Screening (HCS) technique was used to investigate P2Y1R mediated signal transduction processes based on the distribution of fluorescent receptors or the β-arrestin2/receptor complex. All procedures including aggregation, endolysis and resensitization that were involved in the interaction between the P2Y1 receptor and its ligand can be visualized using the tGFP labeled receptor combined with HCS technology in this study. ADP activated P2Y1R to induce a distinguished pattern of fluorescent vesicle formation inside both cell types. These two cell lines produced different peak fluorescence signals when activated by ADP at 100 μM, and the time to form the maximal vesicles was detected as 15 and 20 min respectively. The EC50 values of the concentration–response curves of ADP induced formation of fluorescent vesicles were 35.7 nM and 71.8 nM respectively. The ADP effects could be blocked by a P2Y1R specific antagonist MRS2179. The P2Y1R are coupled to Gq protein to activate PLC first, PLC cleave PIP2 into IP3 and DAG. And then DAG and Ca2+ activate PKC pathway, and finally the phospholipase D initiates β-arrestin2 mediated signal pathway. It took 30 and 45 min to complete P2Y1R re-sensitization in these two constructs respectively. Our results demonstrated that P2Y1R triggered downstream responses not only via heterotrimeric Gq proteins but also through β-arrestin2.
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Batteries & Supercaps
·
2022
·Vol. 6
·期 3
·e202200434
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IF 6.043
Han Wang,Wentao Zou,Chao Liu,Yi Sun,Yi Xu,Weiwei Sun,Yong Wang
Graphical Coordination strategy has been adopted to fabricate the Co ion intercalated two-dimensional COF composite, which delivers improved lithium-storage performances and mechanism as the anode for lithium-organic batteries. As a new kind of crystalline porous polymers, covalent organic frameworks (COFs) exhibit great potential as electrode materials for rechargeable metal-ion batteries due to their stable and adjustable structure, high porosity and abundant electrochemical active centers. In this paper, facile one-step synthesis process is proposed to obtain the cobalt ion intercalated two-dimensional β -ketoenamine-linked COF (Co−DAAQ−TFP-COF, denoted as Co-COF). The intercalation of Co ion in adjacent two layered structure of COF via coordination effects can activate the Li + storage ability of aromatic ring in the original COF, greatly improve the utilization of redox active sites, shorten the migration length of electrons and ions, and promote fast lithium reaction kinetics. Adopted as the anode for lithium-ion batteries, substantial improvement on the reversible capacity and cycling performance can be achieved for the obtained Co intercalated COF electrode (780 mAh g −1 after 200 cycles at 100 mA g −1 ) compared to original COF electrode (120 mAh g −1 under the same condition). The two-dimensional COF materials with morphology justification and/or performance improvement via metal ion intercalation would promote the application of COF related electrodes for other energy-storage fields.
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Experimental and Therapeutic Medicine
·
2023
·Vol. 27
·期 1
·1-13
·
IF 2.7
Qiankun Quan,Xinxin Ma,Ming Li,Xi Li,Haifeng Yuan
β‑Amyloid peptide (Aβ) deposition in the brain is an important pathological change in Alzheimer's disease (AD). Insulin‑degrading enzyme (IDE), which is regulated transcriptionally by peroxisome proliferator‑activated receptor γ (PPARγ), is able to proteolyze Aβ. One of the members of the MAPK family, ERK, is able to mediate the phosphorylation of PPARγ at Ser112, thereby inhibiting its transcriptional activity. Ginsenoside Rg1 is one of the active ingredients in the natural medicine ginseng and has inhibitory effects on Aβ production. The present study was designed to investigate whether ginsenoside Rg1 is able to affect the regulation of PPARγ based on the expression of its target gene, IDE, and whether it is able to promote Aβ degradation via inhibition of the ERK/PPARγ phosphorylation pathway. In the present study, primary cultured rat hippocampal neurons were treated with Aβ1‑42, ginsenoside Rg1 and the ERK inhibitor PD98059, and subsequently TUNEL staining was used to detect the level of neuronal apoptosis. ELISA was subsequently employed to detect the intra‑ and extracellular Aβ1‑42 levels, immunofluorescence staining and western blotting were used to detect the translocation of ERK from the cytoplasm to the nucleus, immunofluorescence double staining was used to detect the co‑expression of ERK and PPARγ, and finally, western blotting was used to detect the phosphorylation of PPARγ at Ser112 and IDE expression. The results demonstrated that ginsenoside Rg1 or PD98059 were able to inhibit primary cultured hippocampal neuron apoptosis induced by Aβ1‑42 treatment, reduce the levels of intra‑ and extraneuronal Aβ1‑42 and inhibit the translocation of ERK from the cytoplasm to the nucleus. Furthermore, administration of ginsenoside Rg1 or PD98059 resulted in attenuated co‑expression of ERK and PPARγ, inhibition of phosphorylation of PPARγ at Ser112 mediated by ERK and an increase in IDE expression. In addition, the effects when PD98059 to inhibit ERK followed by treatment with ginsenoside Rg1 were found to be more pronounced than those when using PD98059 alone. In conclusion, ginsenoside Rg1 was demonstrated to exert neuroprotective effects on AD via inhibition of the ERK/PPARγ phosphorylation pathway, which led to an increase in IDE expression, the promotion of Aβ degradation and the decrease of neuronal apoptosis. These results could provide a theoretical basis for the clinical application of ginsenoside Rg1 in AD.
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CERAMICS INTERNATIONAL
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2023
·
IF 5.2
Xiang Li,Hanxu Zhang,Hang Zhang
Although β-TCP bioceramics possess favorable biocompatibility and bioactivity, their inherent brittleness restricts further applications. In this study, we aimed to enhance the mechanical performance and biocompatibility of β-TCP by incorporating 15% Akermanite (AK) into the structure. The fabrication process involved the use of digital light processing (DLP) technology to fabricate ceramic green bodies, followed by high-temperature debinding and sintering to obtain the ceramic scaffold. Subsequently, we employed an in-situ growth technique to create micro/nano surface topography on the scaffolds, facilitating synergistic regulation of cell behavior in conjunction with the controlled release of bioactive agents. Scanning electron microscopy (SEM) revealed that the addition of AK enlarged the grain size and reduced the number of micropores in the composite scaffold. Furthermore, after the in-situ growth treatment, a significant increase in micro/nano surface features was observed. Mechanical testing demonstrated that the incorporation of AK effectively improved the mechanical properties of the composite scaffold, resulting in an increase in strength of approximately 20%. Cell experiments indicated that AK supplementation further enhanced the material's cell compatibility, and the presence of micro/nano structures effectively promoted cell adhesion, proliferation, and expression of osteogenic genes. Consequently, we propose a strategy for fabricating porous bioceramics that stimulate bone growth, exhibit high strength, and ameliorate the degradation microenvironment. This approach opens up promising avenues for future applications in bone tissue engineering.
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FOOD CHEMISTRY
·
2023
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IF 8.8
Zhikun Yang,Mingrui Li,Yanxiao Li,Xiaowei Huang,Zhihua Li,Xiaodong Zhai,Jiyong Shi,Xiaobo Zou,Jianbo Xiao,Yue Sun,Megan Povey,Yunyun Gong,Melvin Holmes
The poor biological, mechanical and water-resistance properties of sodium alginate/guar gum film (SG) limit its application in food preservation. To overcome this disadvantage, we added β-Cyclodextrin/persimmon pectin-stabilized baobab seed oil Pickering emulsion (BOPE) to enhance the mechanical and water resistance properties of SG film, and added green synthesized silver nanoparticles (AgNPS) and Lycium ruthenicum extract (LA) to improve the biological properties of the film. The properties of BOPE was optimized using Box–Behnken design (BBD). Scanning electron microscope and Fourier transform infrared results revealed the change of structure and molecular interaction in the SG film after the addition of AgNPS, LA, and optimized BOPE. The 2.0%BOPE-loaded film containing AgNPS/LA with the enhanced mechanical, barrier, BO retention, and biological properties not only improved the preservation effect on mushroom ( A. bisporus ), but also maintained structural stability. Thus, the 2.0%BOPE-loaded SG/LA/AgNPS film has considerable potential in active packaging applications.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2023
·
IF 9.9
Zhichun Si,Mengxi Pei,Yishui Liu,Bo Li,Feiyu Kang
Photocatalytic degradation is considered as the most energy-efficient, environmentally benign, and effective method for treating low fraction organic contaminants. However, the photocatalysts still suffer from low utilization efficiency of visible-light and severe carrier recombination. Heterojunctions can resolve these two main problems in some extent but still be restrained by the low quality of hetero-interface. In this study, homojunction was constructed of β-FeOOH quantum dots and nanorods with the same lattice by a two-step precipitation method, to avoid the heterointerface with too many defects and possess good charge separation as a consequence. The catalysts were characterized by activity test, electron spin resonance, Mott-Schottky plots, photocurrent density tests and open-circuit potential measurements, etc. The results revealed that a strong internal electric fields (IEFs) was created at the interface of catalyst. Beneficently, the electron rearrangement leads to a more rational distribution of oxygen vacancies in the catalyst, resulting in more efficient dissociation of oxygen molecules and formation of active radicals, thus facilitating the efficient degradation of toluene. This study proposes a novel strategy to boosting the photocatalytic activity of low dimensional semiconductors via forming homojunction interfaces to improve their charge transfer.
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JOURNAL OF ETHNOPHARMACOLOGY
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2023
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IF 5.4
Yiyang Du,Tingxu Yan,Bo Wu,Bosai He,Ying Jia
Ethnopharmacological relevance Increased inflammatory response and disruption of neuroplasticity are important mechanisms in the hypothesis of the pathogenesis of depression. Thus, these two aspects are conducive to the development of treatments for depression. Suanzaoren Decoction (SZRD) is a classic traditional Chinese medicine compound for the treatment of insomnia, which can clinically relieve depression symptoms, but its antidepressant pharmacological mechanism remains to be elucidated. Aim of this study Based on the hypothesis of inflammation and neuroplasticity in depression, this study aimed to investigate the antidepressant effect of SZRD and its specific molecular mechanism through chronic unpredictable mild stress (CUMS) induced SD rat model and lipopolysaccharide (LPS) induced BV2 cell neuroinflammation model. Materials and methods The body weight and behavioral indexes of CUMS model rats treated with orally or without oral SZRD for 4 weeks were detected. Hematoxylin and eosin staining was used to observe brain pathological damage. Terminal-deoxynucleoitidyl Transferase Mediated Nick End Labeling (TUNEL) staining was used to observe neuronal apoptosis. Immunofluorescence, ELISA kit and Western blotting were used to detect the inflammatory index Iba-1 and inflammatory factors, as well as the important inflammatory pathway TLR4/MyD88/NF-κB. Enzyme linked immunosorbent assay (ELISA) and western blotting were used to detect neuroplasticity indexes proteins-brain-derived neurotrophic factor (BDNF), presynaptic membrane protein-synaptophysin (SYP), and postsynaptic protein- 95(PSD95), and the key pathway Wnt/β-catenin. The possible mechanism of SZRD antidepressant was further explored in LPS-induced BV2 cells. Results In vivo and in vitro experiments showed that SZRD treatment significantly reversed the depression-like behaviors in rats, decreased the levels of inflammatory factors and increased the expression levels of BDNF, SYP, PSD95 in depression model rats. Furthermore, SZRD treatment inhibited the activation of TLR4/MyD88/NF-κB and Wnt/β-catenin pathways and reduced the massive nuclear translocation of NF-κB and β-catenin. The addition of NF-κB pathway agonists could partially offset the inhibitory effect of SZRD on the Wnt pathway, and the addition of Wnt pathway agonists could also partially offset the inhibitory effect of SZRD on the TLR4 pathway. Conclusion This study suggestted that SZRD may exert its antidepressant effect by regulating TLR4/MyD88/NF-κB pathway and Wnt/β-catenin pathway in combination.
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LWT-FOOD SCIENCE AND TECHNOLOGY
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2023
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IF 6
Ya Liu,Yueying Zhang,Feiwu Long,Jinrong Bai,Yina Huang,Hong Gao
An abundance of low-salt sausages had emerged in the market as high salt intake may pose health risks to humans while their limited shelf-life becomes an urgent issue. In this study, tea-polyphenol/β-cyclodextrin/NaCl inclusion complexes (ICs) with thermal stability and better antioxidant activity were successfully prepared, their effects on the quality of low-salt sausages were investigated. The sausages were manufactured with the addition of ICs and NaCl with or without tea-polyphenols respectively, and they were stored at 25 °C for 28 days. IC group exhibited significantly lower oxidation of lipids and protein as the tea-polyphenols in the ICs could exert better antioxidant properties because of the special encapsulation structure. Besides, ICs could inhibit the growth of pathogens preventing the off-flavor. Also, ICs promoted the growth of P. pentosaceus with a strong antioxidant capacity in the sausage model, which maintained the existing pleasant flavor by preventing the oxidation of phenol and alcohol compounds. Meanwhile, ICs also accelerated the growth of Tetragenococcus , which contributed to flavor development at the later stages of fermentation by producing more esters. Hence, it was suggested that ICs could be utilized as a promising salt replacement to improve the quality of traditional Sichuan-style sausages.
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MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Qian Tan,Chao Chen,Cuixia Lin,Jiahui Zhang,Saiwen Liu,Jin Zhang
Kaempferol (KA), which is a biologically active natural polyphenol compound with considerable medicinal value, can be used to fight various diseases. Herein, a β-cyclodextrin/fullerene-graphene oxide/Nickel-metal–organic framework (β-CD/C 60 -GO/Ni-MOF) composite-based electrochemical sensor was developed to detect KA; the composite was characterized using scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, and X-ray photoelectron (XPS) spectroscopy. The biocompatibility and host–guest recognition performance of β-cyclodextrin (β-CD); the porosity and the catalytic performance of the nickel-metal–organic framework (Ni-MOF); and the excellent electrical conductivity of fullerene-graphene oxide (C 60 -GO) work together to considerably improve the detection performance of the sensor for KA. The sensor has an extensive linear range (0.5–50 μM) and a low detection limit (58 nM) with an electrochemically active specific surface area of 0.0971 cm 2 ; furthermore, the redox mechanism of KA in the sensor is explained. For the detection of actual natural samples of broccoli containing KA, satisfactory recovery rates and accuracy were observed; the recovery rate was 96.4 %–101.1 %.
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MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 8
·1-12
·
IF 5.7
Gao Lidi,Cui Hongshou,Guo Xinyu,Dong Qing,You Xingyu,Guo Xinshu,Qin Shili,Jia Lihua
A zeolitic imidazolate framework (ZIF)-8-silica hybrid monolithic column was prepared by one-step sol–gel method. The stationary phase in the monolithic column was characterized by Fourier-transform infrared spectra, X-ray diffraction, thermogravimetric analysis, nitrogen adsorption/desorption, and zeta potential. The results showed that ZIF-8-silica hybrid monolithic materials had abundant functional groups, good crystallinity, large specific surface area, and good thermal stability. A capillary electrochromatography (CEC) chiral separation system was for the first time constructed with ZIF-8-silica hybrid monolithic column and sulfobutylether-β-cyclodextrin (SBE-β-CD) as a chiral additive and was applied to separate the selected single and mixed chiral compounds (13 natural amino acids and 5 chiral pesticides). Under the optimized CEC conditions, all the single analytes achieved baseline separation with resolution of 2.14–5.94 and selectivity factor of 1.06–1.49 in less than 6 min, and the mixed amino acids with similar properties were also simultaneously enantioseparated (Rs ≥ 1.82). Relative standard deviations (RSDs) of migration time and column efficiency were lower than 4.26% and did not change significantly after 200 runs, evidencing excellent reproducibility and stability. These results demonstrate that the application of SBE-β-CD as a chiral additive for ZIF-8-silica hybrid monolithic columns is a promising method for the separation of chiral compounds. Graphical Abstract
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危险品化学品经营许可证（带存储）