ONCOGENE
·
2022
·Vol. 42
·期 7
·530-540
·
IF 8.756
Shi Feiyu,Liu Gaixia,Lin Yufeng,Guo Cosmos liutao,Han Jing,Chu Eagle S. H.,Shi Chengxin,Li Yaguang,Zhang Haowei,Hu Chenhao,Liu Ruihan,He Shuixiang,Guo Gang,Chen Yinnan,Zhang Xiang,Coker Olabisi Oluwabukola,Wong Sunny Hei,Yu Jun,She Junjun
Appendectomy impacts the homeostasis of gut microbiome in patients. We aimed to study the role of appendectomy in colorectal cancer (CRC) risk through causing gut microbial dysbiosis. Population-based longitudinal study (cohort 1, n = 129,155) showed a 73.0% increase in CRC risk among appendectomy cases throughout 20 years follow-up (Adjusted sub-distribution hazard ratio (SHR) 1.73, 95% CI 1.49–2.01, P < 0.001). Shotgun metagenomic sequencing was performed on fecal samples from cohort 2 ( n = 314). Gut microbial dysbiosis in appendectomy subjects was observed with significant enrichment of 7 CRC-promoting bacteria ( Bacteroides vulgatus, Bacteroides fragilis, Veillonella dispar, Prevotella ruminicola, Prevotella fucsa, Prevotella dentalis, Prevotella denticola ) and depletion of 5 beneficial commensals ( Blautia sp YL58, Enterococcus hirae, Lachnospiraceae bacterium Choco86, Collinsella aerofaciens, Blautia sp SC05B48 ). Microbial network analysis showed increased correlation strengths among enriched bacteria and their enriched oncogenic pathways in appendectomy subjects compared to controls. Of which, B. fragilis was the centrality in the network of the enriched bacteria. We further confirmed that appendectomy promoted colorectal tumorigenesis in mice by causing gut microbial dysbiosis and impaired intestinal barrier function. Collectively, this study revealed appendectomy-induced microbial dysbiosis characterized by enriched CRC-promoting bacteria and depleted beneficial commensals, signifying that the gut microbiome may play a crucial role in CRC development induced by appendectomy.
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Advanced Composites and Hybrid Materials
·
2022
·Vol. 6
·期 1
·1-14
·
IF 11.806
Xiao Lin,Xu Weichang,Huang Lixia,Liu Jili,Yang Guang
Effective postoperative therapy is crucial for preventing tumor recurrence and metastasis. Nanocomposite (NC) pastes of Gel/CS-g-CD NP with unique viscoelastic properties and pH-responsive drug delivery ability were prepared from gelatin (Gel) and β-cyclodextrin-grafted chitosan nanoparticles (CS-g-CD NPs) through host–guest interactions between the aromatic amino acid residues of gelatin and β-cyclodextrin. The dynamic rheological properties, shear strength, and shear viscosity were modulated through variations in the concentration of CS-g-CD NPs in the NC pastes. Due to the reversibility of the host–guest interactions, the NC pastes exhibited shear-thinning and self-healing properties, endowing them with good injectability. Loading of doxorubicin (DOX) in the nanoparticles (DOX-CSCD NPs) forming the NC pastes of Gel/DOX-CSCD NP allowed pH-triggered DOX release in a simulated tumoral microenvironment, because of the labile nature of the CS-g-CD NPs under acidic conditions. In vitro experiments showed that the NC pastes of Gel/DOX-CSCD NP induced sustained tumor cell inhibition. Moreover, in vivo studies with tumor-bearing mice indicated that the NC pastes of Gel/DOX-CSCD NP can effectively inhibit tumor recurrence after surgical resection. Therefore, the NC pastes described in this work may act as an effective injectable drug delivery system for postoperative tumoral treatment.
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Nature Communications
·
2022
·Vol. 13
·期 1
·1-22
·
IF 17.694
Zhou Tian,Li Yuxin,Li Xiaoyu,Zeng Fanzhuo,Rao Yanxia,He Yang,Wang Yafei,Liu Meizhen,Li Dali,Xu Zhen,Zhou Xin,Du Siling,Niu Fugui,Peng Jiyun,Mei Xifan,Ji Sheng-Jian,Shu Yousheng,Lu Wei,Guo Feifan,Wu Tianzhun,Yuan Ti-Fei,Mao Ying,Peng Bo
Microglia are important immune cells in the central nervous system (CNS) that undergo turnover throughout the lifespan. If microglial debris is not removed in a timely manner, accumulated debris may influence CNS function. Clearance of microglial debris is crucial for CNS homeostasis. However, underlying mechanisms remain obscure. We here investigate how dead microglia are removed. We find that although microglia can phagocytose microglial debris in vitro, the territory-dependent competition hinders the microglia-to-microglial debris engulfment in vivo. In contrast, microglial debris is mainly phagocytosed by astrocytes in the brain, facilitated by C4b opsonization. The engulfed microglial fragments are then degraded in astrocytes via RUBICON-dependent LC3-associated phagocytosis (LAP), a form of noncanonical autophagy. Interference with C4b-mediated engulfment and subsequent LAP disrupt the removal and degradation of microglial debris, respectively. Together, we elucidate the cellular and molecular mechanisms of microglial debris removal in mice, extending the knowledge on the maintenance of CNS homeostasis.
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Progress in Biomaterials
·
2023
·Vol. 12
·期 2
·75-86
·
IF 4.9
Jafarimanesh Mohammad Amin,Ai Jafar,Shojaei Shahrokh,Khonakdar Hossein Ali,Darbemamieh Goldis,Shirian Sadegh
Hydrogels have been increasingly applied in tissue regeneration and drug delivery systems (DDS). In this study, the capacity of valproic acid (Val) encapsulated within hybrid of alginate (Alg)-chitosan (Cs) (Alg-Cs) hydrogel containing Cs nanoparticle (Npch) with/without human endometrial stem cells (hEnSC) was initially examined for regeneration of spinal cord injury (SCI). To evaluate the stability of the synthesized hydrogels zeta potential necessary measurements were made. Physicochemically, the developed hydrogels were evaluated using Fourier-transform infrared (FTIR) spectroscopy. The physical properties including degradation rate, swelling ability, and tunability of the synthesized hydrogels were studied. To evaluate the nerve regeneration ability of the synthesized hydrogels, 35 Sprague–Dawley rats were undergone SCI. The spinal cords were exposed using laminectomy in T9-T10 area and the hemi-section SCI model was made. The rats were then randomly divided into 5 groups ( n = 7) including, Alg-Cs/Npch, Alg-Cs/Npch/hEnSCs, Alg-Cs/Npch/Val, and Alg-Cs/Npch/hEnScs/Val, and the control groups without any intervention. The FTIR spectra showed band frequencies and assignments of Val, Alg-Cs, and alginate. Nanoparticles were formulated with a mean diameter of 187 and 210 nm, for Val/Alg-Cs and Alg-Cs, respectively. The loading of Val into Alg-Cs led to its reduced size by about 40 nm. The Cs-Npch/Val hydrogels degraded faster than the Alg-Cs-/Npch/Val hydrogel specifically in extended time of incubation. A higher swelling capacity of Alg-Cs/Npch hydrogel, compared to Cs/Npch/Val and Alg-Cs/Npch/Val hydrogels, was found. The Cs-Npch/Val hydrogels degraded faster than Alg-Cs-/Npch/Val hydrogel. The Alg-Cs/Npch/hEnSCs/Val could regenerate the damaged nerve fibers and histologically prevent the SCI-induced vacuolization spaces. The prepared Alg-Cs/Npch/Val could be a suitable polymeric carrier for taurine drugs as bioactive substrate in nerve tissue engineering (NTE) and DDS.
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JOURNAL OF MATERIALS SCIENCE
·
2023
·Vol. 58
·期 5
·2140-2155
·
IF 4.5
Zhang Yeguang,Li Yingchao,Liu Wenxin,Li Feihu,Zhan Zili
CH 4 sensors based on metal oxide semiconductors (MOS) provide high sensitivity and fast response, but the sensitivity will be lost when they are poisoned by silicone. Therefore, there is an urgent need to address the poisoning deactivation of MOS gas sensors caused by silicone. Here, the unique strategy of coating a reactive anti-poisoning material onto the surface of the sensing material is proposed to effectively improve the anti-poisoning ability without degrading the gas sensing performance. As an experimental verification, SnO 2 /α-Al 2 O 3 is designed as the anti-poisoning layer and SnO 2 loaded with precious metals (Pt and Pd) as the sensing layer. Owing to the protection of the anti-poisoning layer, the response ( R a / R g = 24.69) and the response/recovery time (1/24 s) of the sensor to CH 4 remained undiminished at 364 °C after being poisoned under an air/hexamethyldisiloxane (HMDSO) (10 ppm) environment for 40 min, which meets the requirements of the Chinese National Standard (GB 15322.2-2019). The mechanism analysis shows that HMDSO can be decomposed with the adsorbed oxygen on the surface of SnO 2 in the anti-poisoning layer, and CH 4 can diffuse into the sensing layer to be detected. The unique anti-poisoning strategies can provide a promising insight for improving the poisoning tolerance of MOS gas sensors. Graphical abstract
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WATER AIR AND SOIL POLLUTION
·
2023
·Vol. 234
·期 2
·1-13
·
IF 2.9
Wei Yaru,Zhang Kaili,Cheng Tiexin,Zhou Guangdong
In this paper, FeS m /BC composites were prepared using coconut shell biochar (BC) as the carrier. FeS m on FeS m /BC is preferentially oriented along the (001) crystal plane and exhibits a uniform honeycomb morphology. It has a high specific surface area and a small pore size distribution, which is more suitable as a heavy metal ion removal material. The results show that Cr(VI) can be almost completely removed within 120 min under acidic conditions with an initial concentration of 10 mg/L of Cr(VI), a temperature of 20 °C, and a dosage of 0.6 g/L of adsorbent. Where pH was the main factor influencing the removal of Cr(VI):pH affects Cr(VI) removal by changing the form of Cr(VI) in the solution, adsorption free energy, redox potential, charged species on FeS m /BC surface, and the reduction properties of Fe 2+ and S 2− , low pH value is more favorable for Cr(VI) removal. Coexisting anions in solution inhibit Cr(VI) removal due to competitive adsorption, but coexisting cations have no effect. The adsorption process of Cr(VI) by FeS m /BC follows the pseudo-second-order kinetics and Langmuir adsorption isotherm model, indicating that the adsorption process is a monolayer surface adsorption controlled by the chemical adsorption mechanism and involves electron sharing or exchange between adsorbent and adsorbate. Thermodynamic study shows that the adsorption process is a spontaneous entropy-increasing process, and raise the temperature is beneficial to the adsorption. The removal of Cr(VI) by FeS m /BC is the synergistic effect of adsorption, reduction, and co-precipitation.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2023
·Vol. 34
·期 2
·1-10
·
IF 2.8
Gao Lingxiang,Zhang Hao,Liu Wen,Zhang Shulong,Xie Zunyuan
In this study, electro and magneto dual response of TiO 2 @Fe 3 O 4 composite nanoparticles is realized by situ-generating Fe 3 O 4 , which was synthesized by FeCl 3 and FeSO 4 , on rose-like TiO 2 pore in virtue of facile hydrothermal reaction. Morphology, structure and physical properties, such as dielectric constant and magnetic conductivity, of the TiO 2 @Fe 3 O 4 composite particles were characterized. The particles were dispersed in hydrous elastomer to measure their electro and magneto responsive property by the scanning electron microscopy of micro structure of hydrous elastomer and dynamic mechanical analysis that reflected by the sensitivity of the increase in storage modulus ( f = ∆ G / G ) of the hydrous elastomer cured in the absence/presence of external field. The one of the TiO 2 @Fe 3 O 4 particles consisting of the ratio of TiO 2 :Fe 3 O 4 to 2:1 has the highest electro and magneto dual responsive effect that reaches 62%, which is far higher than TiO 2 with only 15%. This work provides a new substance shows the excellent performance of electro and magneto dual response to applied in smart material field.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2023
·Vol. 34
·期 2
·1-10
·
IF 2.8
Guan Yeqing,Zhang Jinxian,Wang Fenglin,Li Wei,Mao Haijun,Liu Zhuofeng,Zhang Weijun,Chen Xingyu
The Ca 0.245 Li 0.325 Nd 0.395 Ti 1− x Sn x O 3 ( x = 0–0.12) ceramics were prepared by the traditional solid-state reaction method. The effects of Sn 4+ substitution for Ti 4+ on the phase composition, microstructure, and microwave dielectric properties of Ca 0.245 Li 0.325 Nd 0.395 Ti 1− x Sn x O 3 (CLNTS x ) were investigated in this work. The X-ray diffraction results showed that the ceramics had a single orthorhombic perovskite structure when x = 0–0.09. However, a secondary phase SnO 2 was observed with x > 0.09. The SEM micrographs indicated that the proper Sn 4+ substitution for Ti 4+ promoted the grain growth and homogenization. The analysis results of Raman spectroscopy revealed the correlation between vibration modes and microwave dielectric properties of CLNTS x ceramics with different Sn 4+ contents. The degree of cation order was related to the full width at half maximum (FWHM) of the Raman spectrum, and a lower FWHM value indicates higher cationic order and Q × f 0 value. The excellent microwave dielectric properties with ε r = 98, Q × f 0 = 3320 GHz, and τ f = 2.6 ppm/°C were obtained in the ceramics with x = 0.09 sintered at 1350 °C for 4 h.
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INFLAMMATION
·
2023
·
IF 5.1
Feng Yong,Hu Jiapeng,Liu Fen,Shang Yunxiao
Asthma is a chronic inflammatory disease characterized by airway remodeling and lung inflammation. Collagen triple helix repeat containing 1 (CTHRC1), a glycoprotein, is involved in multiple pathological processes, including inflammation and fibrosis. However, the function of CTHRC1 in asthma remains unclear. In the present study, the mouse asthma model was successfully generated by sensitizing and challenging mice with ovalbumin (OVA). CTHRC1 expression at both RNA and protein levels was significantly upregulated in lung tissues of asthmatic mice. Asthmatic mice exhibited significant airway remodeling as evidenced by increased bronchial wall and smooth muscle cell layer thickness, goblet cell hyperplasia and collagen deposition, and epithelial-mesenchymal transition (EMT), but those characteristics were reversed by CTHRC1 silencing. The cell model with transforming growth factor-β1 (TGF-β1) induction in bronchial epithelial cells (BEAS-2B) was conducted to verify the effects of CTHRC1 on EMT, a classic mechanism that mediates airway remodeling. The results showed that TGF-β1 stimulation increased CTHRC1 expression, and CTHRC1 knockdown inhibited TGF-β1-induced EMT. OVA-treated mice also showed increased inflammatory cell infiltration and the production of OVA-specific immunoglobulin E (IgE), interleukin (IL)-4, IL-5, and IL-13, which were decreased by CTHRC1 downregulation. The effects of CTHRC1 on OVA-induced airway inflammation were further determined by treating BEAS-2B cells with IL-13, in which CTHRC1 knockdown reduced the IL-13-induced secretion of pro-inflammatory factors, including IL-4 and IL-5. In conclusion, these results indicate that CTHRC1 silencing attenuates asthmatic airway remodeling and inflammation in vivo and in vitro, suggesting that CTHRC1 may be a potential target for asthma treatment. Graphical Abstract
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Nature Communications
·
2023
·Vol. 14
·期 1
·1-10
·
IF 16.6
Jiang Zhao,He Liangrui,Yang Zhiwen,Qiu Huibin,Chen Xiaoyuan,Yu Xujiang,Li Wanwan
Distinctive upconversion or downshifting of lanthanide nanocrystals holds promise for biomedical and photonic applications. However, either process requires high-energy lasers at discrete wavelengths for excitation. Here we demonstrate that co-sensitization can break this limitation with ultrawide excitation bands. We achieve co-sensitization by employing Nd 3+ and Ho 3+ as the co-sensitizers with complementary absorptions from the ultraviolet to infrared region. Symmetric penta-layer core-shell nanostructure enables tunable fluorescence in the visible and the second near-infrared window when incorporating different activators (Er 3+ , Ho 3+ , Pr 3+ , and Tm 3+ ). Transient spectra confirm the directional energy transfer from sensitizers to activators through the bridge of Yb 3+ . We validate the features of the nanocrystals for low-powered white light-emitting diode-mediated whole-body angiography of mice with a signal-to-noise ratio of 12.3 and excitation-regulated encryption. This co-sensitization strategy paves a new way in lanthanide nanocrystals for multidirectional photon conversion manipulation and excitation-bandwidth-regulated fluorescence applications.
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MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 12
·1-10
·
IF 6.408
Sun Min,Wang Xiuqin,Ding Yali,Feng Juanjuan
In order to improve the selectivity and stability of melamine–formaldehyde (MF) aerogel, it was composited with TiO 2 aerogel. A TiO 2 -MF hybrid aerogel was in situ prepared on the surface of carbon fibers for in-tube solid-phase microextraction (SPME). The extraction performance of TiO 2 -MF aerogel was regulated by changing the ratio of TiO 2 sol and MF sol during the material preparation. Coupled with high-performance liquid chromatography-diode array detection (HPLC–DAD), the extraction tube filled by TiO 2 -MF aerogel-coated carbon fibers was evaluated with several types of environmental pollutants including polycyclic aromatic hydrocarbons (PAHs), estrogens, and ultraviolet filters. Because of favourable extraction performance of PAHs they were selected as model analytes, and some important influence factors were optimized for satisfactory sensitivity. The detection limits were in the range 0.05–0.10 μg L −1 , owing to high enrichment factors (653–1007). The online in-tube SPME-HPLC–DAD method was verified for the determination of trace PAHs in environmental water samples, and acceptable recovery (70–118%) was achieved. The analytical methods also displayed some advantages in comparison with other reports. Moreover, the extraction tube exhibited satisfactory chemical stability. Graphical abstract
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PHARMACEUTICAL RESEARCH
·
2022
·Vol. 40
·期 2
·567-577
·
IF 4.58
Chen An,Cai Peishan,Luo Minqian,Guo Minshan,Cai Ting
Purpose The fixed-dose combination drug products have been increasingly used to treat some complex diseases. A cocrystal containing two therapeutic components, named as a drug-drug cocrystal, is an ideal solid form to formulate as a fixed-dose combination product. The aim of the study is to prepare celecoxib-carbamazepine (CEL-CBZ) cocrystals by melt crystallization to achieve the synchronized release of drugs. Method The crystal structure of the CEL-CBZ cocrystal was determined from the cocrystals harvested from melt by single crystal X-ray diffraction. The binary phase diagram and crystal growth kinetics of the CEL-CBZ cocrystal from melt were studied to optimize the process parameters of hot-melt extrusion for manufacturing large-scale cocrystals. The intrinsic dissolution rate studies were conducted to compare the dissolution profiles of drugs in the cocrystal and their individual forms. Result The CEL-CBZ cocrystal crystallized in the triclinic space group with one CEL and one CBZ molecule in the asymmetric unit. The crystallization of CEL-CBZ cocrystals were observed both in the supercooled liquid and glassy state. The formation of drug-drug cocrystals significantly alter the intrinsic dissolution rates of the parent drugs to favor the synchronized release. Conclusion Melt crystallization is an alternative, efficient and eco-friendly approach for preparing drug-drug cocrystals on a large scale. The synchronized drug release by drug-drug cocrystals can be used to modulate the release profiles of parent drugs in the fixed-dose combination products.
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