JOURNAL OF MOLECULAR MEDICINE-JMM
·
2025
·
IF 4.2
Wen Xin,Peng Yuefeng,Yang Wenqing,Zhu Yuzhong,Yu Fan,Geng Li,Wang Xianfeng,Wang Xiaoyan,Zhang Xiaodong,Tang Yi,Feng Lei,Zhou Tingting,Jia Hongliang,Yang Liu
Transient receptor potential canonical 1 (TRPC1) channel, a Ca 2+ -permeable ion channel widely expressed in vasculature, has been reported to be involved in various cardiovascular disorders. However, the pathophysiological function of vascular smooth muscle cell (VSMC)-derived TRPC1 in hypertension and hypertensive cardiovascular remodeling remains to be defined. In this study, we found increased TRPC1 expression in both angiotensin II (AngII)-treated VSMCs and aortas from AngII-infused mice. VSMC-specific TRPC1 deficiency strikingly attenuated AngII-induced vasoconstriction, hypertension, vascular remodeling, and cardiac hypertrophy. Mechanistically, AngII activated enhancer of zeste homolog 2 (EZH2) to stimulate TRPC1 expression, induced calcium influx and phosphorylation of mitogen-activated protein kinase/extracellular signal-regulated kinase (MEK-ERK), which in turn triggered VSMC proliferation and migration and exacerbated hypertension and cardiovascular remodeling. Treatment with EZH2 inhibitor reduced VSMC proliferation and migration and alleviated vasoconstriction and hypertension in AngII-infused mice. Together, we revealed the pathogenic role of the EZH2-TRPC1-MEK/ERK pathway in AngII-induced hypertension and cardiovascular damage. TRPC1 or EZH2 inhibition may represent a desirable therapeutic target for the treatment of hypertension. Key messages
AngII activates AT1R-EZH2-TRPC1 pathway in VSMCs and aortas of hypertensive mice.
TRPC1 promotes VSMC proliferation and migration via MEK/ERK signaling.
Inhibition of TRPC1 or EZH2 alleviates hypertension and cardiovascular remodeling.
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高影响因子
JOURNAL OF POLYMERS AND THE ENVIRONMENT
·
2025
·
IF 5
Xie Shuangyu,Xie Yuhui,Lan Lugui,Wu Feng,Feng Dong,Meng Yang,Mei Yi,Xie Delong
In recent years, the development of bio-based flame retardants for flexible polyurethane foam (FPUF) has become essential, particularly due to environmental and health concerns associated with traditional petrochemical-derived additives. This study reports the successful synthesis of a novel bio-based flame retardant, NMDPLA, using renewable precursors: meglumine, phytic acid, and phenylboronic acid. NMDPLA was combined with protonated chitosan and ammonium polyphosphate through electrostatic self-assembly to create an intumescent flame retardant (APCS). The incorporation of NMDPLA and APCS notably enhances both mechanical properties and flame retardancy of FPUF. The formulation containing 40 wt% NMDPLA in total polyol and 3.0 wt% APCS achieved a Limiting Oxygen Index (LOI) of 23.6, showed no droplet formation in UL-94 tests, and produced a complete carbon residue. Cone calorimetry results demonstrated reductions in total heat release (THR) and peak heat release rate (PHRR) by 22.65% and 20.48%, respectively, compared to pure foam. Balancing mechanical performance and flame retardancy poses a significant challenge, as traditional additives often compromise one for the other. This work underscores the necessity of developing bio-based alternatives that effectively address this balance, enhancing fire safety while preserving material integrity and supporting the transition to sustainable solutions.
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Journal of Food Measurement and Characterization
·
2025
·
IF 3.3
Jing Jing,Li Huizhen,Li Cheng,Xu Mengjie,Zhao Yana,Cui Jianlan,Zhang Zhijun
Hemerocallis citrina Baroni ( H. citrina Baroni) is widely used in the food and medical industries. However, a comprehensive evaluation of the physicochemical, rheological properties, and hypoglycemic activities of its various parts has not yet been reported. In this study, we extracted polysaccharides from the leaves (HCLP) and stems (HCSP) of H. citrina Baroni. The physicochemical properties were investigated using high-performance gel permeation chromatography (HPGPC), high performance liquid chromatography (HPLC), Congo red experiment, Fourier transform infrared spectroscopy (FTIR), ultraviolet-visible spectroscopy (UV-Vis), X-ray diffraction (XRD), thermogravimetric (TG), and scanning electron microscopy (SEM). HCSP exhibited higher total sugar contents, uronic acids, protein, total phenol, and flavonoid contents than HCLP. However, the HCSP extraction yield (2.54 ± 0.68%) was lower than that of HCLP (6.34 ± 0.75%). Both polysaccharides were mainly composed of Mannose (Man) and galactose (Gal), but their contents varied significantly; HCSP contained more glucosamine (GlcN), rhamnose (Rha), arabinose (Ara), gluconic acid (GlcA), galacturonic acid (GalA) and glucose (Glc) than HCLP. The results showed that both HCSP and HCLP displayed macromolecular characteristics with a triple-helix structure. The thermal stability was stronger in HCLP than in HCSP. The rheological results showed that HCLP and HCSP exhibited a pseudoplastic fluid behavior. Compared to HCSP, HCLP had a stronger ability to inhibit α -glucosidase and enhance glucose consumption in HepG2 cells, attributed to its superior thermal stability and higher molecular weight ( M w ). These results indicated that polysaccharides from the leaves and stems of H. citrina Baroni have potential as functional foods and hypoglycemic drugs.
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METABOLIC BRAIN DISEASE
·
2025
·Vol. 40
·期 5
·1-14
·
IF 3.5
Zhao Hexiang,Yang Ping,Zhang Mou,Zheng Wenshu,Qi Renli,Zhu Xiaofeng,Li Jinghui,Li Shipeng
Reperfusion, while essential for restoring blood supply, paradoxically exacerbates neuronal damage through cerebral ischemia-reperfusion injury (CIRI). This study aimed to develop a reactive oxygen species (ROS)-responsive drug delivery system (DDS) loaded with edaravone (EDA) to enhance targeted therapy for CIRI. The stimuli-responsive DDS was synthesized using dextran (DEX) as the biocompatible carrier and benzeneboronic acid pinacol ester (BAPE) as the ROS-sensitive moiety. The physicochemical characteristics of the DEX-BAPE/EDA (DB/EDA) micelles were systematically evaluated. In vitro studies assessed the anti-inflammatory, antioxidant, and anti-apoptotic effects of DB/EDA. Moreover, the neuroprotective efficacy of DB/EDA in vivo was analyzed via behavioral tests, infarct volume measurement, ELISA assays of inflammatory cytokines and OS markers, and Western blot analysis of Nrf2-related pathways. Pharmacokinetics and biosafety were analyzed through plasma profiling and H&E staining. DB/EDA exhibited high stability, efficient drug encapsulation, and ROS-responsive drug release. Cellular uptake studies confirmed enhanced internalization of DB/EDA micelles in BV2 cells. In the oxygen-glucose deprivation/reoxygenation (OGD/R) model, DB/EDA significantly suppressed TNF-α, IL-1β, IL-6, and MDA, restored SOD levels, and attenuated apoptosis. In the middle cerebral artery occlusion/reperfusion (MCAO/R) mice, DB/EDA administration effectively improves cognition and mitigates neuronal damage. Mechanistically, DB/EDA activated the Nrf2/HO-1 pathway, amplifying antioxidant and anti-inflammatory responses. Pharmacokinetic analysis revealed prolonged circulation and increased brain accumulation, and histopathological analysis demonstrated the safety profile of DB/EDA. The ROS-responsive DB/EDA nano-micelles provided targeted EDA delivery to ischemic brain regions, alleviating CIRI via Nrf2 activation, suggesting that DB/EDA is a promising strategy for CIRI treatment. Graphical abstract
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JOURNAL OF POLYMERS AND THE ENVIRONMENT
·
2025
·
IF 5
Yang Yi-jie,Dou Qiang
Two bio-based plasticizers derived from castor oil: raw butyl ricinoleate (RBR) and castor oil glycidyl ether (COGE) were used to plasticize polylactic acid (PLA) by one-step melt blending method. Compatibility analyses revealed that the generation of the branched products between epoxide groups of COGE and carboxyl groups of residual ricinoleic acid (RA) in the RBR and PLA improved the compatibility of the blends. The blends were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), rheology, thermogravimetry (TG), differential scanning calorimetry (DSC), polarized light microscopy (PLM), contact angle measurement and tensile test. The results showed that COGE could act as a compatibilizer and interact with carboxyl groups in RBR and PLA, significantly improving the interfacial compatibility, thermal stability and ductility of the blends. The generation of the entanglements among the molecular chains weakened the crystallization capacity of the ternary blends. The extension of molecular chains and entanglement of branched chains also increased the viscosity of the blends. Thanks to the synergistic application of RBR and COGE, the strain at break of PLA/RBR/COGE (85/3.75/11.25) blends reached 146%, which was 14.9 times larger than that of pure PLA. These blends presented excellent mechanical performance and processing ability, and thus have application scenarios in biodegradable packages and articles. Graphical
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Scientific Reports
·
2025
·Vol. 15
·期 1
·1-16
·
IF 3.9
Dong Yuqi,Qiao Qing-an,Wang Ruping,Yang Yueyue,Cai Honglan,Gao Hongwei
Environmental pollution caused by industrial waste is an increasing concern, and the design and development of efficient photocatalysts offer promising approaches to address this issue. A series of novel Zn 0.6 Cd 0.4 S/ZnO/g-C 3 N 4 composites were prepared through a calcination‒hydrothermal method. Under visible light, these materials demonstrated exceptional performance in the photodegradation of methylene blue (MB), rhodamine B (RhB), and tetracycline (TC). Among the composite materials, Z 0.6 C 0.4 S/ZnO/CN-35% exhibited the highest degradation efficiencies for MB (98.52%), RhB (99.45%), and TC (98.20%), demonstrating excellent reusability as well. The photocatalytic performance of the catalysts was assessed across various water sources and different pH conditions. The findings revealed that the degradation efficiency for contaminants in seawater, lake water, and tap water closely mirrored that observed in deionized water, suggesting wide adaptability among different aqueous solutions. The exceptional photocatalytic performance of the composite materials can be attributed primarily to the efficient separation and migration of holes (h + ) and electrons (e − ) via well-contacted interfaces. Free radical capture experiments confirmed that hydroxyl radicals (·OH) and superoxide ion radicals (·O 2 - ) play crucial roles in the reaction process. The photocatalytic mechanism was also revealed via density functional theory (DFT) calculations. This work introduced an innovative strategy to design and fabricate novel photocatalysts, emphasizing their extensive applications from both theoretical and experimental aspects.
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Journal of Sustainable Metallurgy
·
2024
·Vol. 10
·期 4
·2630-2645
·
IF 2.5
Wu Zi-wang,Wang Lai-xin,Li Jing-wei,Lin Yin-he,Wang Da-long,Zhang Lin,Fan Yu-hang,Tang Wen-ming,Chen Hong-sheng
Vanadium titanomagnetite (VTM) is a complex polymetallic symbiotic mineral dominated by iron, titanium, and vanadium. Iron extraction from VTM is an issue for the utilization of Vanadium titanomagnetite concentrate. In this work, flotation method combined with magnetic sorting was proposed to separate and recover the iron resources from VTM. The reverse cationic flotation of iron ore in VTM was carried out with polyetheramine (PEA, molecular weight of 2000) as collector, and the differences of iron grade and recovery in VTM with different particle sizes were studied. The magnetic separation result shows that the iron grade of the magnetic part is 28.38% with the particle size 100–74 μm, which is 10% higher than the iron grade of the raw ore. The flotation results of magnetic separation products show that PEA-2000 can effectively separate and recover iron from VTM. The iron grade of the magnetic part is increased to 47.27% after flotation process at particle size 100–74 μm, pulp pH 4, PEA-2000 concentration 1 × 10 −4 mol/L, and pulp concentration 10 g/L. The mechanism of flotation separation of Fe from VTM by PEA-2000 was analyzed by zeta potential test, FT-IR test, and XPS test. PEA is adsorbed on the surface of silicate in VTM by electrostatic adsorption and hydrogen bond adsorption, and the iron phase minerals are mutually repulsive, which realizes the separation of iron. The model of iron separation by flotation of PEA-2000 was established. Graphical
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MICROCHIMICA ACTA
·
2024
·Vol. 192
·期 1
·1-11
·
IF 5.3
Yang Kaige,Fu Wenchang,Ma Yinjie,Wu Mingyuan,Li Xinyan,Wang Yan
A straightforward capillary isoelectric focusing (cIEF) method is established with a isoelectric point (pI) range spanning from 3.5 to 7.0, exhibiting excellent linearity and repeatability, with an R 2 value of 0.9937 and migration time RSDs for all standard proteins below 0.3%. Subsequently, this method was applied to model exosomes derived from cell lines and healthy human serum, and the peak attributions of exosomes were identified using DiI labeled exosomes and lysed exosomes. The reproducibility of this method in exosome detection was also validated, as the RSDs of all pI values were less than 1%. Then, we observed a significant increase in the pI of exosomes with higher cholesterol content, irrespective of whether they originated from cell culture medium or mouse plasma. Notably, serum exosomes from healthy volunteers exhibited higher pI values compared to those from hepatocellular carcinoma patients, suggesting a potential diagnostic perspective for cancer. These findings underscore the significance of pI measurement in reflecting modifications in exosomal lipid membrane composition and their implications in biological functions mediated by exosomes. Graphical
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高影响因子
JOURNAL OF FLUORESCENCE
·
2025
·
IF 3.1
Zhao Linxiu,Shi Junli,Liu Yongzheng,Zhao Yue,Yang Siqi,Cao Duanlin,Li Shengling
In this study, a benzothiazole-based fluorescent probe, (methyl ( E )-2-(3-(benzo[ d ]thiazol-2-yl)-2-hydroxy-5-me$thylbenzylidene) hydrazine-1-carboxylate) ( DYH) , was investigated for the possibility of sensitive detection of potentially threatening explosives (2, 4, 6-trinitrophenol) TNP and its application. The probe exhibited blue-green fluorescence in methanol solution and its intensity decreased significantly with increasing TNP concentration, achieving a fluorescence quenching efficiency of up to 99.77%. The binding between the probe and TNP followed a stoichiometric ratio of 1:1, achieving a detection limit of approximately 2.1 × 10 –7 M, indicating its high detection sensitivity. Further studies have shown that the DYH was excellent in rapidly detecting TNP, and its application in natural water samples and solid phase papers validated its usefulness. In addition, this study successfully combined the probe DYH with smartphone technology, establishing a convenient smartphone sensing platform that provides a more convenient solution for rapid and real-time detection of TNP.
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Discover Oncology
·
2025
·Vol. 16
·期 1
·1-10
·
IF 2.9
Gong Qineng,Zhu Tianyu,Zhang Linlin,Wu Hui,Miao Yang,Hu Ye
Hepatocellular carcinoma (HCC) is a predominant form of liver cancer and one of the leading causes of cancer-related death globally. Therefore, there is an urgent need for innovative therapeutic strategies that target the molecular mechanisms underlying HCC progression and metastasis, aiming to improve treatment efficacy and patient survival. The natural product celastrol (Cel) has demonstrated inhibitory effects in various cancer cell lines. However, its clinical application has been hindered by high toxicity and a low safety threshold. Metal-free quantum dots (QDs), AgInS 2 (AIS QDs) not only eliminate toxic risks associated with heavy metals but also exhibit high biocompatibility in the biomedical field. By developing AIS QD@Cel, an AIS QDs nano-delivery system for Cel, the cell selectivity and inhibitory effects of Cel on HCC were enhanced. Fourier-transform infrared spectroscopy (FTIR) analysis revealed that AIS QDs can interact with Cel via amide bonds. The encapsulation rate of AIS QDs to Cel reached 27.5%. AIS QD@Cel eliminated toxicity on 293T and enhanced inhibition on HCC cells by over 10 times. Furthermore, the western blotting and flow cytometry experiments showed that AIS QD@Cel promoted apoptosis and autophagy signal pathway. Finally, transcriptome sequencing revealed that AIS QD@Cel effect on HCC by regulating gene expression involved in critical signaling pathways that are implicated in the progression of cancer. This strategy holds the potential to increase safety threshold and clinical applicability of Cel, offering significant clinical value for the treatment of HCC patients.
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-14
·
IF 15.7
Liu Yinxia,Zhao Lingyun,Long Jinshan,Huang Zhenye,Long Ying,He Jianjun,Jiang Jian-Hui
Synthetic genetic circuits that harness programmable protein modules and artificial transcription factors (ATF) to devise event-triggerable cascaded pathways represent an essential class of tools for studying cell biology. Fine-tuning the general structural functionality of ATFs is important for constructing orthogonal and composable transcriptional regulators. Here, we report the design of a protease-responsive conformationally inhibited system (PRCIS). By intramolecularly linking the free DNA-binding domains of ATF to confined dimerized regions, the transcriptional binding is conformationally inactivated. The function of DNA binding is reinstated upon proteolytic cleavage of linkages, activating the downstream gene expressions. The versatility of PRCIS design is demonstrated through its adaptability to various ATFs and proteases, showcasing high activation ratios and specificity. Furthermore, the development of PRCIS-based triple-orthogonal protease-responsive and dual-orthogonal chemical-inducible platforms and Boolean logic operations are elaborated in this paper, providing a generalizable design for synthetic biology.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2025
·Vol. 23
·期 1
·1-23
·
IF 12.6
Zuo Kangqing,Li Aonan,Si Taoning,Lei Weiyi,Liu Yusheng,Zhang Linbo,Zhang Taixing,Xiao Guiyong,Lu Yupeng,Li Ningbo
Phosphate conversion coatings on metallic implants can synergistically integrate functional components and structural regulation, offering excellent biocompatibility and osteogenic activity. However, the passive oxide layer on the titanium (Ti) surface impedes the following chemical reactivity, adversely affecting the microstructure and properties of phosphate coatings. This study proposes a strategy for achieving structural optimization and properties enhancement of strontium-zinc phosphate (SrZnP) conversion coatings on Ti via regulating interface chemical reaction between coatings and Ti substrates. The results indicated that Sr 2+ and Zn 2+ ions-preloading (IPL) treatment enhanced the interfacial reactivity, which can further achieve crystal refinement and uniform crystal size in nucleation. In contrast, microstructural modifications on Ti substrates induced by acid etching, sandblasting, and alkali etching had minimal effects on the phase composition and crystal morphology (irregular cubic) of the SrZnP coatings. The coatings on IPL-Ti exhibited better mechanical properties and corrosion resistance. Besides, the coatings with optimized structures and surface characteristics elicited bacterial growth inhibition rates of 91.09% and 84.04% against Staphylococcus aureus ( S. aureus ) and Escherichia coli ( E. coli ), respectively. Meanwhile, the crystal-refined coatings further significantly enhanced the adhesion, proliferation, and differentiation of bone marrow mesenchymal stem cells (BMSCs), proving anticipated osteogenic activity. Overall, the ions preloading strategy on variable micro/nanostructured Ti substrates facilitates the potential application of Sr/Zn-phosphate conversion coatings for repairing infected bone defects.
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