Cell Death Discovery
·
2024
·Vol. 10
·期 1
·1-14
·
IF 6.1
Sun Bao-Ye,Wang Zhu-Tao,Chen Ke-Zhu,Song Yang,Wu Jing-Fang,Zhang Dai,Sun Guo-Qiang,Zhou Jian,Fan Jia,Hu Bo,Yi Yong,Qiu Shuang-Jian
Lymph node metastasis (LNM) facilitates distant tumor colonization and leads to the high mortality in patients with intrahepatic cholangiocarcinoma (ICC). However, it remains elusive how ICC cells subvert immune surveillance within the primary tumor immune microenvironment (TIME) and subsequently metastasize to lymph nodes (LNs). In this study, scRNA-seq and bulk RNA-seq analyses identified decreased infiltration of dendritic cells (DCs) into primary tumor sites of ICC with LNM, which was further validated via dual-color immunofluorescence staining of 219 surgically resected ICC samples. Tumor-infiltrating DCs correlated with increased CD8 + T cell infiltration and better prognoses in ICC patients. Mechanistically, β-catenin-mediated CXCL12 suppression accounted for the impaired DC recruitment in ICC with LNM. Two mouse ICC cell lines MuCCA1 and mIC-23 cells were established from AKT/NICD or AKT/YAP-induced murine ICCs respectively and were utilized to construct the footpad tumor LNM model. We found that expansion and activation of conventional DCs (cDCs) by combined Flt3L and poly(I:C) (FL-pIC) therapy markedly suppressed the metastasis of mIC-23 cells to popliteal LNs. Moreover, β-catenin inhibition restored the defective DC infiltration into primary tumor sites and reduced the incidence of LNM in ICC. Collectively, our findings identify tumor cell intrinsic β-catenin activation as a key mechanism for subverting DC-mediated anti-tumor immunity in ICC with LNM. FL-pIC therapy or β-catenin inhibitor could merit exploration as a potential regimen for mitigating ICC cell metastasis to LNs and achieving effective tumor immune control.
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高影响因子
SN Applied Sciences
·
2020
·Vol. 2
·期 7
·1-10
·
IF 0
Wang Yating,Liu Bin,Zhang Wei,Shao Changhong,Lan Daoyun,Qu Xiaofeng,Chen Rongfeng,Zhang Weiqing,Zhao Weimin,Liu Jun,Zhang Yinghe,Shi Zhicong
Lithium–sulfur battery with high theoretical capacity becomes the subject of recent attention. Its commercial progress is impeded by its poor electrical conductivity and high dissolubility of intermediate products in organic electrolyte. In the present work, we report a novel three-dimensional graphene-wrapped porous carbon (3D-G) to accommodate sulfur, which consists of outside highly stable interconnected graphene-like sheets and inner activated porous carbon. The 3D-G was synthesized from cheap polyacrylic acid cation-exchange resin, which introduces nickel ions as catalysts in the carbonization process, as a cost effective, facile and simple method. This 3D-G showed hierarchical pores: 0.7 nm micropores and macropores, providing a large specific surface area of 1375.08 m 2 g −1 . Benefiting from this unique structure, the 3D-G performed well as host materials to achieve a high sulfur content (75.4 wt%). Such a 3D-G@S composite exhibits capacity-fading rate as low as 0.28% per cycle over 100 cycles at 0.1 C, and good cyclability at various cycling rates (0.1–1 C).
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JOURNAL OF NANOBIOTECHNOLOGY
·
2020
·Vol. 18
·期 1
·1-16
·
IF 6.518
Zhou Xia,Wu Hongwei,Long Ruimin,Wang Shibin,Huang Haiwang,Xia Yanhua,Wang Pei,Lei Yifeng,Cai Yuanyuan,Cai Duanhua,Liu Yuangang
Background The traditional treatment for diabetes usually requires frequent insulin injections to maintain normoglycemia, which is painful and difficult to achieve blood glucose control. Results To solve these problems, a non-invasive and painless oral delivery nanoparticle system with bioadhesive ability was developed by amphipathic 2-nitroimidazole– l -cysteine–alginate (NI–CYS–ALG) conjugates. Moreover, in order to enhance blood glucose regulation, an intelligent glucose-responsive switch in this nanoparticle system was achieved by loading with insulin and glucose oxidase (GOx) which could supply a stimulus-sensitive turnover strategy. In vitro tests illustrated that the insulin release behavior was switched “ ON ” in response to hyperglycemic state by GOx catalysis and “ OFF ” by normal glucose levels. Moreover, in vivo tests on type I diabetic rats, this system displayed a significant hypoglycemic effect, avoiding hyperglycemia and maintaining a normal range for up to 14 h after oral administration. Conclusion The stimulus-sensitive turnover strategy with bioadhesive oral delivery mode indicates a potential for the development of synthetic GR-NPs for diabetes therapy, which may provide a rational design of proteins, low molecular drugs, as well as nucleic acids, for intelligent releasing via the oral route.
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高影响因子
MEDICINAL CHEMISTRY RESEARCH
·
2017
·Vol. 26
·期 3
·672-679
·
IF 1.277
Liu Zhenbao,Wang Xiaohong,Zhang Hang,Zhang Shanshan,Li Yiqian,Liu Yanfei,Peng Dongming
Berberine owns multiple pharmacological activities, especially anti-inflammatory activity. To enhance the anti-inflammatory therapeutic efficiency of berberine, a series of novel 9- O -substituted berberine derivatives ( 5a – 5d ) were synthesized and their anti-inflammatory activities were evaluated. 13 C-NMR, 1 H-NMR, IR and high resolution mass spectrum (HRMS) results indicated that these derivatives were successfully synthesized. The xylene-induced inflammatory mice model demonstrated that these derivatives showed dose-dependent inhibition of ear inflammatory swelling. Comparing to berberine, ibuprofen and naproxen-modified berberine derivatives enhanced anti-inflammatory activities, while aspirin and nicotinic acid-modified berberine derivatives showed lower anti-inflammatory effects at the same dosages. This could be attributed to the enhanced inhibition of secretion of cytokines, including interleukin-6 and tumor necrosis factor-α, by ibuprofen and naproxen berberine derivatives, while aspirin and nicotinic acid berberine derivatives showed lower inhibition of tumor necrosis factor-α than berberine. These results suggest that ibuprofen and naproxen-modified berberine derivatives are promising new anti-inflammatory drug candidates.
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高影响因子
AMERICAN JOURNAL OF POTATO RESEARCH
·
2020
·Vol. 97
·期 4
·367-375
·
IF 0.858
Daurov Dias,Daurova Ainash,Karimov Aibek,Tolegenova Dana,Volkov Dmitriy,Raimbek Daniyar,Zhambakin Kabyl,Shamekova Malika
Potato ( Solanum tuberosum L.) is the fourth most common food crop worldwide and one of the main food products in Kazakhstan. One of the factors leading to crop loss in potato is viral disease. There are more than 30 viruses known to cause diseases in potato. Therefore, using virus-free planting material is an important aspect of growing potato. It is relatively difficult to eliminate viruses from plants, despite the application of isolated apical meristems. In this study, we used the most effective methods to eliminate viral infections, such as electrotherapy, chemotherapy (40 and 50 mg/l), cryotherapy, and thermotherapy. Complete elimination of certain viral infections, such as PVM, PVS, PVX, and PVY, was achieved after chemotherapy with 40 mg/l of ribavirin. A multiplex RT-PCR method was used for efficient monitoring of viral infections, which is more precise than the enzyme-linked immunosorbent assay (DAS-ELISA). The obtained virus-free planting material will subsequently be used for industrial virus-free potato seed production in Kazakhstan.
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高影响因子
MOLECULAR BIOLOGY REPORTS
·
2024
·Vol. 51
·期 1
·1-12
·
IF 2.6
Zheng Ruzhen,He Yibo,Yang Lingrong,Chen Yidan,Wang Rui,Xie Shangnao
Background Our previous study has demonstrated that Nischarin (NISCH) exerts its antitumor effects in breast cancer (BC) by suppressing cell migration and invasion. This study aims to explore the underlying mechanism through which NISCH functions in BC. Methods and results The relevance between EGF Like Repeats and Discoidin Domains 3 (EDIL3) mRNA expression and the overall survival of tumor patients was depicted by the Kaplan-Meier curve. The findings revealed that overexpressed NISCH attenuated cell motility and colony-forming capacities of Hs578T cells, yet silenced NISCH in MDA-MB-231 cells led to contrasting results. Western blot (WB) analysis indicated that overexpression of NISCH significantly down-regulated the Vimentin and Slug expression, and inactivated the FAK/ERK signaling pathway. RNA sequencing (RNA-seq) was performed in NISCH-overexpressed Hs578T cells and the control cells to analyze differentially expressed genes (DeGs), and the results showed a significant down-regulation of EDIL3 mRNA level upon overexpression of NISCH. Subsequent functional analyses demonstrated that overexpression of EDIL3 attenuated the inhibitory effect of NISCH on cell migration, invasion, colony formation, and tube formation. Conclusion In summary, our finding preliminarily revealed that NISCH inhibits the epithelial-mesenchymal transition (EMT) process and angiogenesis in BC cells by down-regulating EDIL3 to inactivate the FAK/ERK signaling pathway, thereby suppressing the progression of BC. Our results hold promise for contributing to the deep understanding of BC pathogenesis and identifying new therapeutic strategies for clinical application.
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高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2021
·Vol. 28
·期 13
·16655-16662
·
IF 4.223
Xiao Jinjing,Ma Jinjuan,Wang Fan,Xu Xing,Liao Min,Shi Yanhong,Cao Haiqun
Numerous natural preparations in traditional Chinese medicine are prepared as decoctions. Processing factors (PFs) comparing the levels of pesticide residues in decoctions to those in the corresponding unprocessed products should be considered in exposure assessments. Thus, this study determined the residue levels of six pesticides (chlorpyrifos, phoxim, imidacloprid, thiamethoxam, fenpropathrin, and emamectin benzoate), as well as 3,5,6-trichloropyridinol, the primary metabolite of chlorpyrifos, and clothianidin, the main metabolite of thiamethoxam in Baishao, Paeoniae radix lactiflora (Fam. Ranunculaceae). The results showed that significant time-response effects were present for the release of pesticides from P. radix. The PFs calculated were < 1, indicating a significant reduction in pesticide residues after TCM processing. The water solubility and partition coefficient values of the pesticides may have played a basic role in the dissipation of the residues during the TCM decocting process. A risk assessment based on the hazard quotient with PFs revealed that exposure to pesticide residues in P. radix was far below the levels that might pose a health risk. In conclusion, the results presented here are of theoretical and practical value for the safety evaluation of TCMs.
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高影响因子
MACROMOLECULAR RESEARCH
·
2021
·Vol. 29
·期 7
·494-504
·
IF 2.227
Li Zhenghan,Chen Junjie,Zhou JinYue,Nie YiWen,Shen Chunhui,Gao Shanjun
The main chain of polyolefin was synthesized by copolymerization of 4 — vinylbenzyl chloride (VBC) and acrylonitrile (AN), and trimethylamine is used for quaternization to prepare heterogeneous benzyl trimethyl-ammonium anion exchange membranes (Heter-X) and homogeneous benzyl trimethyl-ammonium anion exchange membrane (Homo-X). The results of Fourier-transform infrared (FT-IR), gel permeation chromatography (GPC), and 1 H nuclear magnetic resonance (NMR) showed that VBC and AN were successfully copolymerized to form a polymer with a certain molecular weight, and trimethylamine was successfully quaternized. The prepared membranes exhibited good thermal stability and mechanical properties. The theoretical ion exchange capacity (IEC) values of Homo-3 and Heter-3 are the same, but the conductivity at 80 °C were 0.0572 S cm −1 and 0.0505 S cm −1 . The results showed that the homogeneous method has a higher degree of quaternization and a more uniform distribution of quaternary ammonium groups, forming a more obvious microphase separation structure, which can also be seen in the atomic force microscopy (AFM) diagram. After being soaked in 1 M KOH solution for 480 h, the ionic conductivity of Homo-6 and Heter-6 can still remain 91.4% and 85.5%, and the IEC loss rates were 17.21% and 24.34%. These results indicate that the prepared membranes are promising materials for application in fuel cells.
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高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2021
·Vol. 32
·期 11
·14368-14384
·
IF 2.478
Hu Yongcai,Du Zhaofu,Yao Yingbang,Ma Yue,Li Yi,Xu Yiwen,Zhao Xiaobo,Tao Tao,Liang Bo,Lu Shengguo
Silver powders were prepared by a chemical reduction method. Effects of different surfactants during this preparation process were examined. Using these silver powders, silver pastes were prepared for low temperature co-fired ceramics (LTCC) applications. Another batch of different surfactants were added into the silver paste and evaluated. Moreover, various organic thixotropic agents were used to modify the thixotropy behavior of the silver paste. It was found that the surfactants of PVP (Polyvinyl pyrrolidone, for preparation of the silver powder) and Span-85 (for the preparation of silver paste) exhibited the best performance, with an electrical resistivity of 0.11 mΩ mm. The silver paste with the thixotropic agent of hydrogenated castor oil shows the largest thixotropic index of 1.56. Using different organic additives, the microstructural, rheological and electrical properties of the silver paste can be considerably improved, and our results shed light on the optimization of the silver paste for LTCC applications.
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高影响因子
Communications Materials
·
2020
·Vol. 1
·期 1
·1-10
·
IF 0
Jin Zhengyuan,Jiang Xiantao,Zhang Qitao,Huang Shaolong,Zhang Luhong,Huang Lili,He Tingchao,Zhang Han,Ohno Teruhisa,Ruan Shuangchen,Zeng Yu-Jia
Broad-spectrum light activation in photocatalytic materials is considered vital for effective solar-to-hydrogen energy conversion. Here, we propose an upconversion process in oxygen-doped polymeric carbon nitride (C 3 N 4 ) nanosheets, resulting in high photocatalytic activity for hydrogen generation from water splitting under infrared light irradiation. Due to the upconversion, the photoexcited electrons are transferred to the conduction band, and a σ* carbon-oxygen orbital localized on the triazine matrix ring resulting from oxygen doping extends the lifetime of photogenerated electrons, which increases the photocatalytic activity under both infrared and visible light. Our material exhibits high apparent quantum efficiency of 0.014% and 23% at 850 nm and 420 nm, respectively. Under infrared light (λ ≥ 800 nm, 24 mW cm −2 ), the hydrogen production rate of our material is 22.84 μmol h −1 g −1 , which can reach a yield similar to that of pristine C 3 N 4 under visible light.
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RARE METALS
·
2024
·
IF 9.6
Feng Yu-Shi,Shen Yu,Wang Fang,Tian Chuan,Hu Qin-Yi,Duan Qian,Li Jing-Wei,Bao Di
Perovskite oxides with diverse composition and structure have exhibited grand advances in boosting the oxygen reduction and evolution reaction (ORR/OER), which are essential for the reversible protonic ceramic electrochemical cell (R-PCEC) toward the sustainable hydrogen production and utilization. However, enhancement of their activity and stability remains challenging. Herein, we develop the Ta-regulated BaCo 0.7 Fe 0.3 O 3− δ perovskite oxygen electrode (Ba(Co 0.7 Fe 0.3 ) 1− x Ta x O 3− δ ) with abundant oxygen defects and achieve the simultaneous enhancement in the electrocatalytic activity and stability toward ORR and OER. As-fabricated R-PCEC with Ba(Co 0.7 Fe 0.3 ) 0.9 Ta 0.1 O 3− δ (BCFT10) oxygen electrode performs high power density of 1.47 W·cm −2 at 650 °C in fuel cell mode, and the current density is up to − 2.11 A·cm −2 at 1.4 V at 650 °C in electrolysis mode, as well as the good stability in both the fuel cell and electrolysis modes. Importantly, the cell also demonstrates a stable cycling operation between fuel cell and electrolysis mode, suggesting a great potential of BCFT10 as oxygen electrode material for R-PCECs. Graphical abstract
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高影响因子
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2024
·
IF 2.6
Li Xiang,Pan Zhihu,Ji Xiaohong
MXenes are promising two-dimensional materials for energy storage applications, but the stacking and aggregation of MXene nanosheets degrade their electrochemical performance. In this work, three-dimensional (3D) porous Ti 3 C 2 T x was successfully prepared on carbon cloth with controllable mass loading, porosity, and pore size with an electrophoretic co-deposition (EPCD) strategy by introducing polystyrene (PS) microspheres as soft sacrificial templates. The resulting 3D porous structure exhibits a significant enhancement in electrochemical performance due to effectively suppressing the stacking and aggregation of Ti 3 C 2 T x nanosheets, which provides additional electron transport channels and electrochemically active sites. The modified 3D porous Ti 3 C 2 T x @CC shows a specific capacitance as high as 299 F g −1 (about 1.6 times that of pristine Ti 3 C 2 T x ) at a current density of 1 A g −1 , a satisfactory capacitance of 234 F g −1 (approximately 78.3% of the initial capacitance) under a high current density of 10 A g −1 , and excellent cycling stability. Furthermore, a porous Ti 3 C 2 T x @CC-based symmetric supercapacitor displays an energy density of 6.6 µWh cm −2 (3.9 Wh kg −1 ) at a power density of 0.2 mW cm −2 (125 W kg −1 ). This work facilitates an effective EPCD strategy for producing 3D porous Ti 3 C 2 T x for energy storage, electrocatalysis, and environmental applications.
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高影响因子
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