BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
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2023
·
IF 5.1
Ziyuan Tong,Yuan Shen,Quan Yuan,Honghao Yu
Despite recent advances in understanding the biological behavior of osteosarcoma (OS), OS is still the most common primary bone sarcoma that endangers the health of children and adolescents. High-temperature requirement A (HTRA) protease family plays an important regulatory role in numerous malignancies and acts as a prognostic biomarker. However, the function and underlying mechanisms of the HTRA family in OS development remain unknown. Through analyzing the GSE126209 dataset obtained from different Gene Expression Omnibus (GEO) databases, we found that HTRA3 as a member of the HTRA family was downregulated in OS tissues compared with that in normal tissues. Functional experiments indicated that HTRA3 overexpression suppressed malignant behaviors of OS cells in vitro and tumor growth in vivo . Mechanistically, we found that HTRA3 co-localized with the X-linked inhibitor of apoptosis protein (XIAP) and decreased XIAP stability. Further investigation showed that XIAP knockdown inhibited the degradation of phosphatase and tensin homolog (PTEN) and that HTRA3 caused the blockage of PTEN/phosphoinositide 3-kinase (PI3K)/AKT pathway, characterized as the reverse of cell function caused by HTRA3 overexpression after PTEN inhibitor BpV (HOpic) treatment. Detailed investigations showed that forkhead box protein 1 (FOXP1), an oncogene in OS progression, downregulated HTRA3 expression and inhibited the transcriptional activity of HTRA3 , suggesting that HTRA3 was regulated negatively by FOXP1. In conclusion, our study demonstrates that HTRA3 is a repressor involved in OS development via the PTEN/PI3K/AKT pathway under the modulation of transcription factor FOXP1, and it may provide a therapeutic direction for OS patients.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2023
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IF 9.9
Yiqing Li,Manqin Fu,Xiaoxia Zhang,Chun He,Dingsheng Chen,Ya Xiong,Liqing Guo,Shuanghong Tian
Highly reactive nanoclusters of metal oxides are extremely difficult to be synthesized due to their thermodynamic instability. For the first time, CuO x nanoclusters supported on anatase TiO 2 nanotubes (NT) with many defects as anchoring sites were successfully prepared. Although the copper loading reached as high as 2.5 %, the size of CuO x nanoclusters in the sample of 2.5 %CuO x /NT were mainly around 1.0 nm. The aggregation of copper species during the calcination process was undoubtedly hampered by the anchoring effects of the abundant defects in NT support. Due to the highly exposed undercoordinated atoms of CuO x nanoclusters, the mixed valences of copper, and the strong interface interaction between CuO x nanoclusters and NT support, 2.5 %CuO x /NT-catalyzed ozonation showed the highest pseudo-first-order reaction rate constant of 8.5 × 10 −2 min −1 , 2.2 and 4.0 times that of NT-catalyzed ozonation and ozonation alone, respectively. Finally, the catalytic mechanism was revealed by both experiments and density functional theory calculations (DFT). The results demonstrated that the undercoordinated Cu in CuO x /NT could highly promote the adsorption of ozone with a high adsorption energy of −125.16 eV and the adsorbed ozone was activated immediately, tending to dissociate into a O 2 molecule and a surface O atom. Thus, abundant reactive oxygen species , e.g., hydroxyl radical (·OH), superoxide radical (·O 2 – ) and singlet oxygen ( 1 O 2 ), could be generated via chain reactions. Especially, ·OH mainly contributed to the removal of ibuprofen pollutants. This work sheds a light on the design and preparation of highly reactive nanoclusters of metal oxide catalysts for catalytic ozonation of refractory organic pollutants .
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MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 8
·1-8
·
IF 5.7
Xu Feifei,Li Xinyang,Chen Chen,Liang Zhigang,Xu Guanhong,Wei Fangdi,Yang Jing,Hu Qin,Cen Yao
A fast, eco-friendly and accurate ratiometric fluorescent strategy is presented for the determination of organophosphorus pesticides (OPs) using intrinsic dual-emission silica nanoparticles modified with Rhodamine 6G (SiNPs-Rho6G). SiNPs-Rho6G had intrinsic dual-emission at 410 and 550 nm. The substrate acetylcholine was catalyzed by acetylcholinesterase (AChE) to produce thiocholine (TCh). TCh triggered the specific reaction of Ellman's reagent 5, 5-dithiobis (2-nitrobenzoic acid) to obtain 5-thio-2-nitrobenzoic acid, which caused the decrease in fluorescence intensity of SiNPs-Rho6G at 410 nm by the inner filter effect, while the fluorescence intensity of SiNPs-Rho6G at 550 nm was not significantly changed. OPs caused the recovery of the fluorescence at 410 nm by inhibiting the activity of AChE. Thus, the quantitative detection of OPs could be achieved through utilizing the catalytic characteristic of AChE. The linear curve from 0.010 to 0.250 μg mL -1 with a detection limit of 7 ng mL -1 was obtained for the determination of chlorpyrifos (Cpf). The ratiometric probe was used to detect the spiked Cpf in environmental and food samples with good recoveries. Therefore, combined with the dual emission characteristics of SiNPs-Rho6G and the specificity of the enzyme, the ratio fluorescence sensing platform has potential application prospects in OPs determinations. Graphical Abstract
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TOXICOLOGY AND APPLIED PHARMACOLOGY
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2023
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IF 3.8
Yalan Cui,Xuejun Chen,Jingjing Shi,Qian Jin,Ruihua Zhang,Tong Shi,Chen Wang,Liqin Li
Pre-administration of huperzine A (Hup A) was validated to prevent poisoning from exposure to nerve agents (NAs) by reversibly inhibiting acetylcholinesterase (AChE). However, like the currently commonly used reversible inhibitors, Hup A has a short half-life and is unable to produce a long-term preventative effect. To extend the protective time of Hup A against NAs, 42 derivatives with a C N bond were designed based on the structure of Hup A in this study. All designed derivatives showed good binding capability with AChE via molecular docking. Six compounds (H3, H4, H11, H14, H16, and H25) with representative structures were selected for synthesis by Schiff base reaction, and their structures were stable. The modified Ellman's method showed the six compounds concentration-dependently inhibited AChE, and the half maximal inhibitory concentration (IC 50 ) were higher than that of Hup A. Pretreatment of AChE with the derivatives significantly increased the IC 50 of soman . In vivo experiments demonstrated H3, H4, H14, H16, and H25 had longer protective capacities against 1 × LD 95 soman-induced death in mice than Hup A. The 12 h protective index showed that the protective ratios of H3, H4, H14 and H16 were 2.31, 1.85, 2.23 and 1.99 respectively, better than that of Hup A. The extended protection of the derivatives against soman may be explained by their transformation to Hup A in vivo. Furthermore, all six compounds showed lower acute oral toxicity than Hup A. Overall, our study provided an optional strategy to acquire pretreatment agents for NAs with extended action and low toxicity.
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Materials Today Bio
·
2023
·
IF 8.2
Lin Huang,Wantao Wang,Yiwen Xian,Lei Liu,Jinghao Fan,Hongmei Liu,Zhaomin Zheng,Decheng Wu
Intervertebral disc (IVD) degeneration occurred with the increasing age or accidents has puzzled peoples in daily life. To seal IVD defect by injectable hydrogels is a promising method for slowing down IVD degeneration. Herein, we reported a rapidly in situ forming injectable chitosan/PEG hydrogel (CSMA-PEGDA-L) through integrating photo-crosslink of methacrylate chitosan (CSMA) with Schiff base reaction between CSMA and aldehyde polyethylene glycol (PEGDA). The CSMA-PEGDA-L possessed a stronger compressive strength than the photo-crosslinked CSMA-L hydrogel and Schiff-base-crosslinked CSMA-PEGDA hydrogel. This chitosan/PEG hydrogel showed low cytotoxicity from incubation experiments of nucleus pulpous cells. When implanted on the punctured IVD of rat's tail, the CSMA-PEGDA-L hydrogel could well retard the progression of IVD degeneration through physical plugging, powerfully proven by radiological and histological evaluations. This work demonstrated the strategy of in situ injectable glue may be a potential solution for prevention of IVD degeneration.
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APPLIED ORGANOMETALLIC CHEMISTRY
·
2023
·Vol. 37
·期 9
·e7212
·
IF 3.9
Fuhua Wei,Meng Nie,Qinhui Ren,Xiang Yu,Hua Li,Hongliang Chen,Mengting He,Zhao Liang,Siyuan Wang,Dongxue Han
This work studies the use of Cu/Co-MOFs for the removal of doxycycline hydrochloride (DOC) in wastewater. Cu/Co-MOFs were prepared using a solvothermal method and characterized by XRD, SEM, and TG. According to the experimental results, the maximum DOC adsorption capacity of Cu/Co-MOFs within 5 h was 268.5 mg/g. The pseudo-second-order (PSO) kinetic model shows that the R 2 are all greater than 0.99, which proves that the theory DOC adsorption by Cu/Co-MOFs is consistent with practice. According to the Freundlich isotherm model, the adsorption of DOC by Cu/Co-MOFs is a multilayer adsorption. Thus, the results show that Cu/Co-MOFs have practical application value in removing DOC.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
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IF 8.6
Zhipeng Luo,Yao Xu,Diyong Tang,Li Lu,Yuanfang Li,Mengmeng Zhang,Jie Sun
For persulfate-based advanced oxidation process, the non-radical pathway is an important route due to the selective oxidation of pollutants and its strong tolerance to complex water bodies. However, the selective oxidation mechanism of organic pollutants needs further elaboration. Herein, we demonstrate a facile hydrothermal pretreatment and high-temperature carbonization route to synthesize the nitrogen-doped porous biochar (NHBC-1). Owing to the well-developed hierarchical porous structure, the created internal active defects, and tailored nitrogen dopants, the NHBC-1 catalyst exhibited excellent catalytic activity for peroxydisulfate (PDS) activation. The results show that pyridinic N facilitated the adsorption of PDS on NHBC-1 to form the highly active NHBC-PDS* complex. Meanwhile, graphitic N and the defective structures promoted the electron-transfer process, thus allowing the efficient catalytic performance of the NHBC/PDS system for phenol oxidation via an electron-transfer dominated pathway. In addition, the system exhibited versatile applicability for the remediation of various organic pollutants in different actual water matrices with wide pH adaptability and satisfactory mineralization efficiency. More importantly, the selective oxidation mechanism of organic contaminants was revealed. Specifically, only the pollutant with oxidation potential lower than that of the NHBC-PDS* complex (0.91 V) can be effectively eliminated. This study not only provides a facile route for the rational designing of a cost-affordable biochar catalyst for PDS activation, but also offers valuable insights into the understanding of selective oxidation of organic contaminants via an electron-transfer dominated pathway.
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ANALYST
·
2023
·
IF 4.2
Juan Yang,Dongmiao Qin,Na Wang,Yusheng Wu,Kanjun Fang,Biyang Deng
The construction of advanced systems capable of accurately detecting neuron-specific enolase (NSE) is essential for rapidly diagnosing small-cell lung cancer. In this study, an electrochemiluminescence (ECL) resonance energy transfer immunosensor was proposed for the ultra-sensitive detection of NSE. The co-reactants C2O42− and Ru(bpy)32+ were integrated to form a self-enhanced ECL luminophore (Ru-ZnMOF) as the ECL donor. The abundant carboxyl functional groups of Ru-ZnMOF supported antibody 1 via an amidation reaction. Polydopamine-modified zinc dioxide nanoflowers, as ECL acceptors, inhibited Ru-ZnMOF ECL signaling. The linear range of NSE was 10 fg/mL to 100 ng/mL with a detection limit of 3.3 fg/mL (S/N = 3), which is suitably low for determining NSE in real samples.
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RSC Advances
·
2023
·Vol. 13
·期 32
·22367-22374
·
IF 3.9
Datian Fu,Yan Wang,Kaiwen Lin,Liangjiu Huang,Jin Xu,Haimei Wu
Although disrupted redox homeostasis has emerged as a promising approach for tumor therapy, most existing photosensitizers are not able to simultaneously improve the reactive oxygen species level and reduce the glutathione (GSH) level. Therefore, designing photosensitizers that can achieve these two aspects of this goal is still urgent and challenging. In this work, an organic activatable near-infrared (NIR) photosensitizer, CyI-S-diCF3, is developed for GSH depletion-assisted enhanced photodynamic therapy. CyI-S-diCF3, composed of an iodinated heptamethine cyanine skeleton linked with a recognition unit of 3,5-bis(trifluoromethyl)benzenethiol, can specifically react with GSH by nucleophilic substitution, resulting in intracellular GSH depletion and redox imbalance. Moreover, the activated photosensitizer can produce abundant singlet oxygen (1O2) under NIR light irradiation, further heightening the cellular oxidative stress. By this unique nature, CyI-S-diCF3 exhibits excellent toxicity to cancer cells, followed by inducing earlier apoptosis. Thus, our study may propose a new strategy to design an activatable photosensitizer for breaking the redox homeostasis in tumor cells.
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REACTIVE & FUNCTIONAL POLYMERS
·
2023
·
IF 5.1
Zhengyue Li,Xiuying Sun,Xiuqiong Chen,Hongcai Wang,Dongze Li,Ting Shang,Linxin Qi,Huiqiong Yan,Qiang Lin
To enhance the applicability of alginate hydrogel in tissue engineering, we attempted to fabricate oxidized sodium alginate/polyacrylamide-gelatin (OSA/PAM-GT) composite hydrogels by interpenetrating network approach with the hydroxyapatite/D-glucono-δ-lactone (HAP/GDL) as the endogenous ionic cross-linking agent for oxidized sodium alginate (OSA) and N , N ′-methylenebisacrylamide (MBAA) as the chemical cross-linking agent for polyacrylamide (PAM), followed by their surface coverage with gelatin. Then TiO 2 nanoparticles as the reinforcing agent were added to the OSA/PAM-GT hydrogel matrix to construct organic/inorganic hybrid OSA/TiO 2 /PAM-GT composite hydrogels. In particular, the effects of the amount of TiO 2 nanoparticles on the microscopic morphology, mechanical properties , swellability, biodegradability , bio-mineralization and biocompatibility of the composite hydrogels were studied. Experimental results indicated that the addition of TiO 2 nanoparticles into the matrix of OSA/TiO 2 /PAM-GT composite hydrogels could effectively regulate the porosity, mechanical properties, swelling ratio, in vitro biodegradability and bio-mineralization of OSA/TiO 2 /PAM-GT composite. Moreo v er, OSA/TiO 2 /PAM-GT composite hydrogels had the best biocompatibility when the content of TiO 2 nanoparticles was 0.5% ( w / v ), indicating that an appropriate amount of TiO 2 nanoparticles could effectively promote cell adhesion, proliferation and differentiation. Consequently, the combined method of the interpenetrating network technology, the incorporation of TiO 2 nanoparticles with the surface coverage of gelatin could overcome the defects of alginate hydrogels to some extent, making OSA/TiO 2 /PAM-GT composite hydrogels suitable for tissue engineering applications.
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INFLAMMATION
·
2023
·
IF 5.1
Xing Zeyu,Zhen Yanhua,Chen Jie,Du Mingyang,Li Dongdong,Liu Ruyin,Zheng Jiahe
Atherosclerosis (AS), characterized by a maladaptive inflammatory response, is one of the most common causes of death among the elderly. Karyopherin subunit alpha 2 (KPNA2), a member of the nuclear transport protein family, has been reported to play a pro-inflammatory role in various pathological processes by regulating the nuclear translocation of pro-inflammatory transcription factors. However, the function of KPNA2 in AS remains unknown. ApoE −/− mice were fed high-fat diets for 12 weeks to establish an AS mice model. Human umbilical vein endothelial cells (HUVECs) were treated with lipopolysaccharide (LPS) to establish an AS cell model. We found that KPNA2 was upregulated in the aortic roots of atherosclerotic mice and LPS-stimulated cells. KPNA2 knockdown inhibited LPS-induced secretion of pro-inflammatory factors and monocyte-endothelial adhesion in HUVECs, whereas KPNA2 overexpression exerted the opposite effects. p65 and interferon regulatory factor 3 (IRF3), the transcription factors known to regulate the transcription of pro-inflammatory genes, interacted with KPNA2, and their nuclear translocations were blocked following KPNA2 silencing. Furthermore, we found that KPNA2 protein level was decreased by E3 ubiquitin ligase F-box and WD repeat domain containing 7 (FBXW7), which was downregulated in the atherosclerotic mice. FBXW7 overexpression induced ubiquitination with subsequent proteasomal degradation of KPNA2. Meanwhile, the effects of KPNA2 deficiency on atherosclerotic lesions were further confirmed by in vivo experiments. Taken together, our study indicates that KPNA2 downregulation, regulated by FBXW7, may alleviate endothelial dysfunction and related inflammation in the progression of AS by suppressing the nuclear translocation of p65 and IRF3.
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Biomaterials Science
·
2023
·
IF 6.6
Weichu Zhu,Jian Xu,Xianxian Yao,Shuting Mai,Dan Shu,Wuli Yang
Pyroptosis is a proinflammatory form of cell death mediated by members of the gasdermin family, and is a powerful tool against cancer. Herein, a pH-responsive doxorubicin (DOX)-encapsulating zeolitic imidazolate framework-8 (ZIF-8) nanoparticle coated with a carboxybetaine-based zwitterionic polymer (DOX@ZIF-8@PCBMA) was prepared. Furthermore, decitabine (DAC) was loaded to obtain a pyroptosis nanotuner (DOX@ZIF-8@PCBMA-DAC). This nanotuner displayed extended blood circulation and enhanced tumor accumulation. In addition, the ZIF-8 structure and disulfide-crosslinked PCBMA coating endowed DOX@ZIF-8@PCBMA-DAC with acidic-pH- and glutathione-responsive degradation. The nanotuner could robustly activate caspase-3 to induce gasdermin E (GSDME)-dependent pyroptosis via the sustained release of DAC and DOX, contributing to excellent tumor suppression with negligible side effects, which may provide novel insights into traditional chemotherapy.
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